NCR57: Reproductive Physiology

(Multistate Research Coordinating Committee and Information Exchange Group)

Status: Inactive/Terminating

Project Menu

SAES-422 Reports

Annual/Termination Reports:

[07/22/2003] [07/15/2004]

Date of Annual Report: 07/22/2003

Report Information

Annual Meeting Dates: 05/21/2003 - 05/22/2003
Period the Report Covers: 05/01/2002 - 05/01/2003

Participants

Mark Diekman Indiana, Tim Safranski Missouri, Lloyd Anderson Iowa, Dave Guthrie USDA-ARS-Beltsville, Joe Ford USDA-ARS-MARC, Duane Davis Kansas, Rod Geisert Oklahoma, Tom Welsh Texas (substituting for Dr. Fuller Bazer and Dr. Tom Spencer), John Parrish Wisconsin, Brett White Nebraska, Deb Hamernik USDA-CSREES-PAS - John Baker - Administrative Advisor - Michigan State University

Brief Summary of Minutes

Minutes Attachment:

Attachment

Accomplishments

1) Scientists involved with the committee continue to identify mechanisms controlling pubertal development, resumption of cycles after parturition, folliculogenesis, gametogenesis, fertilization, pregnancy establishment, and survival of embryos and fetuses. Further, the committee is identifying genes and their products that regulate these processes and developing technologies for in vitro embryo production, enhanced embryo and fetal survival, and regulation of the estrous cycle. Please see Appendix A for summaries of individual reports presented at the 2003 NCR-57 Committee Meeting. 2) Following the success of last years meeting, a compact disc containing all of the presentations from the committees first biennial symposium, the Seasonal Infertility in Swine Symposium, was released to participants. It is anticipated that information on this compact disc will be disseminated to scientists, extension educators and producers alike. 3) The research stations participating in the NCR-57 project published 117 refereed journal articles, 7 technical reports and 27 abstracts during the last reporting period. Many of these publications were based on collaborations established through the North Central Regional Committee on Reproductive Physiology. Plans for the Coming Year: Of utmost priority for the committee is preparation of the proposal for the 5-year renewal of the NCR-57 Committee Research Project (Due December 1, 2003). The committee was overwhelmingly supportive of pursuing the renewal and continuing the highly productive collaborative efforts of the committee. In addition, the committee is planning its second biennial symposium to be held at next years annual meeting on the campus of Iowa State University in Ames, IA. The Symposium on Boar Stud Management and A.I. in Swine will focus on data from boar studs that typically are not reported in refereed journal articles. A poll of industry representatives performed by the organizing committee indicated that this topic needed to be addressed and would be beneficial to producers. Consistent with its objectives, the committee will also continue to explore mechanisms underlying reproductive biology of swine and other domestic food animals through collaborative efforts of its representatives.

Publications

Journal Articles: Alexenko, A.P., C.L. Franklin, A.D. Ealy, and R.M. Roberts. 2003. Effect of three distinct IFN-tau variants on progression and severity of experimental allergic encephalomyelitis (EAE) in mice. J. Immunol. (In Press). Anderson, L.L. 2003. Invited book review of Biology of the Domestic Pig Edited by Wilson G. Pond and Harry J. Mersmann, Cornell University Press, Ithaca, New York, pp 1-745. 2001, hardcover ISBN 0-8014-3468-8, for The Quarterly Review of Biology 78:105-106. Anderson, L.L. and B.P. Jena. 2003. Invited chapter entitled, Fusion pores in growth hormone secreting cells of the pituitary gland: An AFM study. In: AFM in Biology and Medicine. J. Wiley & Sons, Inc., New York. Austin, E.J., M. Mihm, A.C. Evans, J.L. Ireland, J.J. Ireland and J.F. Roche. 2002. Effects of oestradiol and progesterone on secretion of gonadotrophins and health of first wave follicles during the oestrous cycle of beef heifers. Reproduction 124:531-541. Bahr, J.M., A. Rivera, M. Nakai, J. Walsh, G.L.Evans, S. Lotinum, R. T. Turner, M. Black, and E.H. Jeffery. 2003. Effects of dietary soy protein and isoflavones on bone and the reproductive tract of sexually mature ovariectomized Sprague-Dawley rat. Endocrinology (Submitted). Bakke, L.J., M.P. Dow, C.A. Cassar, M.W. Peters, J.R. Pursley and G.W. Smith. 2002. Effect of the preovulatory gonadotropin surge on matrix metalloproteinase (MMP)-14, MMP-2, and tissue inhibitor of metalloproteinase-2 expression within bovine periovulatory follicular and luteal tissue. Biol. Reprod. 66:1627-1634. Belstra, B.A., M.A. Diekman, W.L. Singleton and B.T. Richert. 2002. Effects of lactation length and exogenous progesterone and estradiol 17b regimen during embryo attachment on endogenous steroid concentrations and embryo survival in sows. Theriogenology 57:2063-2081. Bonk, A.J., J.E. Anderson, L.R. Abeydeera, B.N. Day and R.S. Prather. 2002. Cyclin B1 levels in the porcine 4-cell stage embryo. Zygote 10:79-84. Brad, A.M., C.L. Bormann, J.E. Swain, R.E. Durkin, A.E. Johnson, A.L. Clifford and R.L. Krisher. 2003. Glutathione and adenosine triphosphate content of in vivo and in vitro matured porcine oocytes. Mol. Reprod. Dev. 64:492-498. Braundmeier, A.G., J.M. Demers, R.D. Shanks, and D.J. Miller. 2003. The ability of porcine sperm to bind the zona pellucida is related to litter size after insemination. J. Anim. Sci. (Submitted). Braundmeier, A.G., J.M. Demers, R.D. Shanks, R.G. Saacke, and D.J. Miller. 2002. Examination of the binding ability of bovine spermatozoa to the zona pellucida as indicator of fertility. J. Androl. 23:645-651. Burkin, H.R., A.P. Alves-Vieira, and D.J. Miller. 2003. Localization of zona pellucida receptors on live sperm by fluorophore-conjugated solubilized zona pellucida proteins. Meth Molecular Medic. (In Press). Caamaqo, G.M., M.A. Wu, G. Corbalan, T.C. McCauley, A.R. Rieke, T.C. Cantley, B.A. Didion, and B.N. Day. 2002. Transvaginal ultrasound-guided oocyte aspiration in sows: Preliminary results and recent improvement. Theriogenology 57:788. Cassar, C.A., M.P. Dow, J.R. Pursley and G.W. Smith. 2002. Effect of the preovulatory LH surge on bovine follicular progesterone receptor mRNA expression. Dom. Anim. Endocrinol. 22:179-187. Cheong, H.T., K.W. Park, G.S, Im, L.Lai, Q.Y Sun, B.N. Day, R.S. Prather. 2002. Effect of elevated Ca2+ concentration in fusion/activation medium on the fusion and development of porcine fetal fibroblast nuclear transfer embryos. Mol. Reprod. Dev. 61:488-492. Childs, K.D., D.W. Goad, M.F. Allan, D. Pomp, C. Krehbiel, R.D. Geisert, J.B. Morgan and J.R. Malayer. 2002. Differential expression of NAT 1 translational repressor during development of bovine intramuscular adipocytes. Physiol. Genomics 10:49-56. Choi, Y, G.A. Johnson, T.E. Spencer and F.W. Bazer . 2003. Pregnancy and interferon tau regulate major histocompatibility complex class I and beta-2-microglobulin expression in the ovine uterus. Biol Reprod 68:1703-1710. Clamon, L.A., R.A. Nelson, L.M. Pyter and J.M. Bahr. 2003. Induction of pseudopregnancy in the American Black Bear (Ursus americanus). J. Zoo Biol. (In Press). Crichton, E.G., E. Bedows, A.K. Miller-Lindholm, D.M. Baldwin, D.L. Armstrong, L.H. Graham, J.J. Ford, J.O. Gjorret, P. Hyttel, C.E. Pope, G. Vajta, and N.M. Loskutoff. 2003. Efficacy of porcine gonadotropins for repeated stimulation of ovarian activity for oocyte retrieval and in vitro embryo production and cryopreservation in Siberian tigers (Panthera tigris altaica). Biol. Reprod. 68:105-113. De Rensis, F., M. Sottocorona and R.N. Kirkwood. 2002. Effect of injecting prostaglandin and dexamethasone on farrowing and on piglet neonatal growth. Vet. Rec. 151:330-331. De Rensis, F., S. Benedetti, P. Silva and R.N. Kirkwood RN. 2003. Fertility of sows following artificial insemination at a gonadotrophin-induced estrus coincident with weaning. Anim. Reprod. Sci. 76:245-250. DeSousa, P.A., J.R. Dobrinsky, J. Zhu, A.L. Archibald, A. Ainslie, W. Bosma, J. Bowering, J. Bracken, P.M. Ferrier, J. Fletcher, B. Gasparrini, L. Harkness, P. Johnston, M. Ritchie, W.A. Ritchie, A. Travers, D. Albertini, A. Dinnyes, T.J. King, and I. Wilmut. 2002. Somatic cell nuclear transfer in the pig: control of pronuclear formation and integration with improved methods for activation and maintenance of pregnancy. Biol. Reprod. 66:642-650. De Young, R., L.I. Muller, S. Demarais, H.D. Guthrie, G.R. Welch, and T. Engelken. 2003. Offspring sex ratio in deer male reproductive physiology and offspring sex ratio in Odocoileus virginianus. J. Mammalogy (Submitted). Dow, M.P., L.J. Bakke, C.A. Cassar, M.W. Peters, J.R. Pursley and G.W. Smith. 2002. Gonadotropin surge-induced upregulation of the plasminogen activators (tissue plasminogen activator and urokinase plasminogen activator) and the urokinase plasminogen activator receptor within bovine periovulatory follicular and luteal tissue. Biol. Reprod. 66:1413-1421. Echternkamp, S.E. and K.E. Gregory. 2002. Reproductive, growth, feedlot and carcass traits of twin versus single births in cattle. J. Anim. Sci. 80 (E. Suppl 2) E64-E73. Ferrell A.D., J.R. Malayer, K.L. Carraway and R.D. Geisert. 2003. Sialomucin complex (Muc4) expression in porcine endometrium during the oestrous cycle and early pregnancy. Reprod Dom Anim 38:1-3. Fischer-Brown, A., R.L. Monson, J.J. Parrish and J.J. Rutledge. 2002. Cell allocation in bovine embryos cultured in two media under two oxygen concentrations. Zygote 10:341-348. Ford, S.P. 2002. Placental development, Efficiency and Embryonic Mortality. In: Perspectives in Pig Science. Edited by J. Wiseman, M. Varley and B. Kemp. Nottingham University Press, Loughborough (In Press). Geisert, R.D., J.W. Ross, M.D. Ashworth and J.R. Malayer. 2002. Endometrial expression of inter-a-trypsin inhibitor heavy chains in cyclic and pregnant gilts. Reproduction (Submitted). Gray, C.A., R.C. Burghardt, G.A. Johnson, F.W. Bazer, and T.E. Spencer. 2002. Evidence that absence of endometrial gland secretions in uterine gland knockout ewes compromises conceptus survival and elongation. Reproduction 124:289-300. Groothuis, P.G., W.J. McGuire, J.L. Vallet, D.M. Grieger and D.L. Davis. Retinol and estradiol regulation of retinol binding protein and prostaglandin production by porcine uterine epithelial cells in vitro. J. Anim. Sci. 80:2688-2694. Guthrie, H.D., J. Liu, and J.K. Critser. 2002. Osmotic tolerance limits and effects of cryoprotectants on motility of bovine spermatozoa. Biol. Reprod. 67:1811-1816. Guthrie, H.D., and G.R. Welch. 2003. Effects of semen processing and cryopreservation on plasma membrane phospholipid scrambling in boar spermatozoa. Biol. Reprod. (Submitted). Guthrie, H.D., and G.R. Welch. 2003. Increasing the holding time of boar spermatozoa t 15o C before freezing decreases fertility 23 days after insemination. J. Anim. Sci. (Submitted). Henning, H.J., and D.L. Davis. Evaluation of the uterine environment and embryos of prepubertal gilts. J. Anim. Sci. (Submitted). Hernandez, A., and J.M. Bahr. 2003. Role of FSH and EGF in the initiation of steroidogenesis in granulosa cells associated with follicular selection in the chicken ovary. Reproduction 125:683-691. Hernandez, A., H.-C. Yao, and J.M. Bahr. 2002. Extraovarian and intraovarian control of ovarian function in the chicken. In: Perspectives in Avian Endocrinology. A. Dawson (ed). J. Endocrinology, Ltd. Bristol, UK. Hernandez, A.G., and J.M. Bahr. 2002. Culture of chicken granulosa cells from small yellow follicles: a suitable culture system. J. Poultry Sci. 39:14-21. Herrick, J.R., A.M. Brad, R.L. Krisher, and W.F. Pope. 2003. Relationship between intracellular adenosine triphosphate or glutathione concentration and in vivo developmental potential of porcine oocytes. Anim. Reprod. Sci. (In Press). Herrick, J.R., M.L. Conover-Sparman and R.L. Krisher. 2003. Reduced polyspermic fertilization of porcine oocytes utilizing elevated bicarbonate and reduced calcium concentrations in a single medium system. Reprod. Fertil. Dev. (Submitted). Imakawa, K., K. Tamura, R.S.F. Lee, Y. Ji, H. Kogo, S. Sakai, and R.K. Christenson. 2002. Temporal expression of type I interferon receptor in the peri-implantation ovine extra-embryonic membranes: Demonstration that human IFN can bind to this receptor. Endocr. J. 49:195-205. Janzen, F.J., M.E. Wilson, J.K. Tucker and S.P. Ford 2002. Experimental manipulation of steroid concentrations in circulation and in egg yolks of turtles. J. Exp. Zool. 293:58-66. Jimenez-Krassel, F. and J.J. Ireland. 2002. Development and validation of a short-term, serum-free culture system for bovine granulose cells: evaluation of the effects of somatotropin and growth hormone-releasing factor on estradiol production. J. Dairy Sci. 85:68-78. Jimenez-Krassel, F. and J.J. Ireland. 2003. Evidence for a negative intrfollicular role for inhibin in regulation of estradiol production by granulose cells. Endocrinology 144:1876-1886. Jo, M., Komar, C.M. and Fortune, J.E. (2002) Gonadotropin surge induces two separate increases in messenger RNA for progesterone receptor in bovine periovulatory follicles. Biol. Reprod. 67:1981-1988. Kim, K.S., J.M. Reecy, W.H. Hsu, L.L. Anderson, and M.F. Rothschild. 2003. Missense mutations of melanocortin-4 receptor alters adenylyl cyclase activity without affecting ligand-binding and is associated with muscle characteristics in pigs. Endocrinology (Submitted). Killian, G. 2002. Estrogen-associated glycoproteins in oviduct secretions: structure and evidence for a role in fertilization. pp. 187-201. In Introduction to Mammalian Reproduction D. Tulsiani, ed. Kluwer Acad. Pub. Boston. King, T.J., J.R. Dobrinsky, J. Zhu, H.A. Finlayson, W. Bosma, L. Harkness, W.A. Ritchie, A. Travers, C. McCorquodale, B.N. Day, A. Dinnyes, P.A. DeSousa and I. Wilmut. 2002. Embryo development and establishment of pregnancy after embryo transfer in pigs: coping with limitations in the availability of viable embryos. Reproduction. 123:507-515. Knox, R.V., G. Vatzias, C.H. Naber, and D.R. Zimmerman. 2003. Plasma gonadotropins and ovarian hormones during the estrous cycle in high compared to low ovulation rate gilts. J. Anim. Sci. 81:249-260. Knox, R.V., G. Miller, K. Willenburg and S.L. Rodriguez-Zas. 2002. Effect of frequency of boar exposure and adjusted mating times on measures of reproductive performance in weaned sows. J. Anim. Sci. 80:892-899. Lai, L., D. Kolber-Simonds, K.W. Park, H.T. Cheong, J. L. Greenstein, G.S. Im, M. Samuel, A. Bonk, A. Rieke, B.N. Day, C.N. Murphy, D.B. Carter, R.J. Hawley, and R.S. Prather. 2002. Production of alpha1,3-galactosyltransferase knockout pigs by nuclear transfer cloning. Science 295:1089-1092. Lai, L., K.W. Park, H.T. Cheong, B.Kuhholzer, M. Samuel, A. Bonk, G.S. Im, A.R. Rieke, B.N. Day, C.N. Murphy, D. Carter and R.S. Prather. 2002. Transgenic pig expressing the enhanced green fluorescent protein produced by nuclear transfer using colchicine-treated fibroblasts as donor cells. Mol. Reprod. Dev. 62:300-306. Langendijk, P., E.G. Bouwman, A. Kidson, R.N. Kirkwood, N.M. Soede and B. Kemp. 2002. Functions of myometrial activity in sperm transport through the genital tract and fertilisation in sows. Reproduction 123:683-690. Lavery, K., A. Way, and G.J. Killian. 2003. Identification of haptoglobin in oviduct fluid and tissues of the cow reproductive tract. Reproduction (In Press). Lazarus, M. , C.J. Mundy, N. Eguchi, S. Matsumoto, G.J. Killian, B.K. Kubata, and Y. Urade. 2002. Immunohistochemical localization of microsomal PGE synthase-1 and cyclooxygenases in male mouse reproductive organs. Endocrinology 143:2410-2419. Looper, M.L., J.A. Vizcarra, R.P. Wettemann, J.R. Malayer, T.D. Braden, R.D. Geisert, and G.L. Morgan. 2003. Influence of estradiol, progesterone, and nutrition on concentrations of gonadotropins and GnRH receptors, and gene expression for GnRH receptors and gonadotropin subunits in pituitaries of beef cows. J. Anim. Sci. 81:269-278. Lunstra, D.D., T.H. Wise, and J.J. Ford. 2003. Sertoli cells in the boar testis: Changes during development and compensatory hypertrophy after hemicastration at different ages. Biol. Reprod. 68:140-150. Madan, P., P.J. Bridges, C.M. Komar, A.G. Beristain, R. Rajamahendran, J.E. Fortune, and C.D. MacCalman. 2003. Expression of messenger ribonucleic acid for ADAMTS subtypes changes in the periovulatory follicle after the gonadotropin surge and during luteal development and regression in cattle. Biol. Reprod. (Accepted). Mao, J., G. Wu, M.F. Smith, T.C. McCauley, T.C. Cantley, R.S. Prather, B.A. Didion, B.N. Day. 2002. Effects of culture medium, serum type, and various concentrations of follicle-stimulating hormone on porcine preantral follicular development and antrum formation in vitro. Biol. Reprod. 67:1197-1203. Mapes, A., F. Tarantal, C.R. Parker, F.M. Moron, J.M. Bahr, L. Pyter, and A.J. Conley. 2002. Adrenocortical cytochrome b5 expression during fetal development of the Rhesus Macaque. Endocrinology 143:1451-1458. Matlock, B., B. Heffernan, R. Edwards, J.M. Bahr, and S. Maloy. 2003. The role of SefA fimbriae in the pathogenesis of salmonella enteritidis in chicks. J. Anim. Sci. (Submitted). McCoard, S.A., S.C. Fahrenkrug, L.J. Alexander, B.A. Freking, G.A. Rohrer, T.H. Wise, and J.J. Ford. 2002. An integrated comparative map of the porcine X chromosome. Anim. Genet. 33:178-185. McCoard, S.A., T.H. Wise, and J.J. Ford. 2003. Germ cell development in Meishan and White Composite gilts. Anim. Reprod. Sci. 77:85-105. McCoard, S.A., T.H. Wise, and J.J. Ford. 2002. Expression levels of Mullerian-inhibiting substance, GATA4 and 17-hydroxylase/17,20-lyase cytochrome P450 during embryonic gonadal development in two diverse breeds of swine. J. Endocrinol. 175:365-374. McGuire, W.J., K. Imakawa, K. Tamura, C.S. Meka, and R.K. Christenson. 2002. Regulation of endometrial granulocyte macrophage-colony stimulating factor (GM-CSF) in the ewe. Domest. Anim. Endocrinol. 23:383-396. Men, H., J.J.Parrish and J.J.Rutledge. 2003. Detection of DNA damage in bovine metaphase II oocytes resulting from cryopreservation. Mol. Reprod. Dev. 64:245-250. Miller D.J., X. Shi, and H.R. Burkin. 2002. Molecular basis of mammalian gamete binding. Rec. Prog Hormone Res. 57:37-73. Miller, L.A., and G.J. Killian. 2002. In search of the active PZP epitope in white-tailed deer immunocontraception. Vaccine 20:2735-2742. Mitchell, D.C., Ing NH. 2003. Estradiol stabilizes estrogen receptor mRNA in sheep endometrium via discrete sequence elements in its 3‘ untranslated region. Mol. Endocrinol. 17:562-574. Mitchell, K.E., M.L. Weiss, B.M. Mitchell, P. Martin, D.L.Davis, L. Morales, B. Helwig, M. Beerenstrauch, K. Abou-Easa, T. Hildreth, and D. Troyer. 2003. Matrix cells from Whartons jelly form neurons and glia. Stem Cells 21:50-60. Mohan, M., S. Ryder, P.L. Claypool, R.D. Geiesert and J.R. Malayer. 2002. Analysis of gene expression in the bovine blastocyst produced in vitro using suppression-subtractive hybridization. Biol. Reprod. 67:447-453. Moran, F.M., J.J. Ford, C.J. Corbin, S.M. Mapes, V.C. Njar, A.M. Brodie, and A.J. Conley. Regulation of microsomal P450, redox partner proteins and steroidogenesis in the developing testes of the neonatal pig. Endocrinology (Submitted). Murdoch, W.J. 2003. Ovarian responses to undernutrition in pregnant ewes. Reproductive Biology and Endocrinology 143:3361-3369. Musser, R.E., D.L. Davis, M.D. Tokach, J.L. Nelssen, S.S. Dritz, and R.D. Goodband. Fetal and maternal responses to ad libitum feed intake during early gestation. J. Anim. Sci. (Submitted). Musser, R.E., S.S.Dritz, D.L. Davis, M. D. Tokach, J.L. Nelssen, R.D. Goodband, and K.Q. Owen. Additional L-carnitine in the gestating sow diet alters muscle development and improves carcass characteristics of the offspring. J. Anim. Sci. (Submitted). Nakai, M., L. Cook, L. Pyter, M. Black, J. Sibonga, R.T. Turner, E.H. Jeffrey, and J.M. Bahr. 2003. Dietary soy protein and isoflavones: effects on bone and reproductive tract of sexually mature intact Sprague Dawley rats. Endocrinology (Submitted). Parent J., C. Villeneuve, A.P. Alexenko, A.D. Ealy, and M.A. Fortier. 2003. Influence of different isoforms of recombinant trophoblast interferons on prostaglandin production in cultured bovine endometrial cells. Biol. Reprod. 68:1035-1043. Patterson, J.L., H.J. Willis, R.N. Kirkwood and G.R. Foxcroft. 2002. Impact of boar exposure on puberty attainment and breeding outcomes in gilts. Theriogenology 57:2015-2025. Peters, M.W. and J.R. Pursley. 2002. Fertility of lactating dairy cows treated with Ovsynch after presynchronization injections of PGF2 alpha and GnRH. J. Dairy Sci. 85:2403-2406. Ramachandran, A., M. Zhang, D. Goad, G. Olah, J.R. Malayer and Z. El Rassi. 2003. Capillary electrophoresis and fluorescence studies on molecular beacon-based variable length oligonucleotide target discrimination. Electrophoresis 24:70-77. Rawe, V.Y., S. Brugo Olmedo, F.N. Nodar, R. Ponzio, P. Sutovsky. 2003. Abnormal assembly of annulate lamellae and nuclear pore complexes coincides with fertilization arrest at pronuclear stage of human zygotic development. Human Reprod. 18:576-582. Roberts, R.M., T. Ezashi, C.S. Rosenfeld, A.D. Ealy, and H.M. Kubisch. 2003. The interferon-tau: Evolution of the genes and their promoters, and maternal-trophoblast I nteractions in control of their expression. Reproduction (In Press). Ross J.W., J.R. Malayer, J.W. Ritchey and R.D. Geisert. 2003. Involvement of the interleukin-1b system in porcine trophoblastic elongation and at the fetal-maternal interface during peri-implantation development. Biol. Reprod. (Submitted). Ross J.W., M.D. Ashworth, A.G. Hurst, J.R. Malayer and R.D. Geisert. 2003. Analysis and characterization of differential gene expression during rapid trophoblastic elongation in the pig using suppression subtractive hybridization. Reproductive Biology and Endocrinology 1:23. Sartori, R., R. Sartor-Bergfelt, S. A. Mertens, J.N. Guenther, J.J. Parrish and M.C. Wiltbank. 2002. Fertilization and early embryonic development in heifers and lactating cows in summer and lactating dry cows in winter. J. Dairy Sci. 85:2803-2812. Scanes, C.G., and McNabb, A.F. 2003. Avian models for toxicological research. Avian and Poultry Biology Reviews 14:21-52. Schulz, L.C., and J.M. Bahr. 2003. Glucose-6-phosphate isomerase stimulates embryo implantation in the domestic ferret. J. Zoo Biol. (Submitted). Sherrer, E.S., T.J. Rathbun, and D.L. Davis. 2003. Fertilization and blastocyst development in oocytes obtained from prepubertal and adult pigs. J. Anim. Sci. (Submitted). Shipka, M P., J.E. Rowell and S.P. Ford. 2002. Reindeer bull introduction affects the onset of the breeding season. Anim. Reprod. Sci. 72:27-35. Simpson, K.S., C.M. Komar, T.E. Curry, Jr. 2003. Localization and Expression of Tissue Inhibitor of Metalloproteinase-4 (TIMP-4) in the Immature Gonadotropin-Stimulated and Adult Rat Ovary. Biol. Reprod. 68:214-221. Sun, H.S., L.L. Anderson, T.-P. Yu, K.-S. Kim, J. Klindt and C.K. Tuggle. 2002. Neonatal Meishan pigs show POU1F1 genotype effects on plasma GH and PRL concentration. Anim. Reprod. Sci. 69:223-237. Sutovsky, P., T.C. McCauley, M. Sutovsky, and B.N. Day. 2003. Early degradation of paternal mitochondria in domestic pig (Sus scrofa) is prevented by selective proteasomal inhibitors lactacystin and MG 132. Biol. Reprod., 68:1793-1800. Sutovsky, P. 2003. Ubiquitin-dependent proteolysis in mammalian spermatogenesis, fertilization, and sperm quality control: killing three birds with one stone. Micros. Res. Tech., 61:88-102. Sutovsky, P., R.M. Turner, S. Hameed, M. Sutovsky. 2003. Differential ubiquitination of stallion sperm surface proteins: possible implications for infertility and reproductive seasonality and infertility. Biol. Reprod. 68:688-698. Sutovsky, P., E. Neuber, and G. Schatten. 2002. Ubiquitin-dependent, sperm quality control mechanism recognizes spermatozoa with DNA defects, as revealed by dual ubiquitin-TUNEL assay. Mol. Reprod. Dev. 61:406-413. Swain, J.E., C.L. Bormann, S.G. Clark, E.M. Walters, M.B. Wheeler and R.L. Krisher. 2002. Use of energy substrates by various stage preimplantation pig embryos produced in vivo and in vitro. Reproduction 123:253-260. Swain, J.E., C.L. Bormann and R.L. Krisher. Metabolism of glucose, pyruvate and glutamine by porcine oocytes matured in vitro and in vivo. Reproduction (In Press). Taitzoglou, I.A., D.A. Chapman, and G.J. Killian. 2003. Induction of the acrosome reaction in bull spermatozoa with plasmin. Andrologia (In Press). Talbot, N.C., T.J. Caperna, and K.D. Wells. 2002. The PICM-19 cell line as an in vitro model of liver bile ductules: effects of cAMP inducers, biopeptides, and pH. Cell Tissue Organs 171:99-116. Talbot, N.C., A.M. Powell, and W.M. Garrett. 2002. Spontaneous differentiation of porcine and bovine embryonic stem cells into astrocytes and neurons. In Vitro Cellular and Dev Biol. 38:191-197. Tarocco, C. and R.N. Kirkwood. 2002. Vaginal length is not related to subsequent litter size of gilts. J. Swine Health Prod. 10:125-126. Ten Haff, W., P.A. Thacker and R.N. Kirkwood. 2002. Effect of injecting gonadotrophins during the luteal phase of the estrous cycle on the inter-estrus interval of gilts. Can. J. Anim. Sci. 82:457-459. Thompson, W.E., J. Ramalho-Santo, and P. Sutovsky. 2003. Ubiquitination of prohibitin in mammalian sperm mitochondria: Possible roles in the regulation of mitochondrial inheritance and sperm quality control. Biol. Reprod. (In Press). Troyer, D.L., M.L. Weiss, K.E. Mitchell, P.L. Martin, and D.L. Davis. 2003. Incorporation of bovine bone marrow stromal cells into porcine fetal tissues after xenotransplantation. Anatomia Histologia Embryologia 32:98-101. Vallet, J.L., K.A. Leymaster, and R.K. Christenson. 2002. The influence of uterine function on embryonic and fetal survival. J. Anim. Sci. 80 (E. Suppl. 2):E115-E125. Vonnahme K.A., S.C. Ferando, J.W. Ross, U. DeSilva, J.R. Malayer and R.D. Geisert. 2003. Porcine endometrial and conceptus expression of kininogen, plasma kallikrein and factor XII in cyclic and pregnant gilts. Reproduction (Submitted). Wang, W., B.N. Day. 2002. Development of porcine embryos produced by IVM/IVF in a medium with or without protein supplementation: effects of extracellular glutathione. Zygote 10:109-115. Way, A.L., and G.J. Killian. 2002. Capacitation and induction of the acrosome reaction in bull spermatozoa with norepinephrine. J. Androl. 23:352-357. Wijayagunawardane, M.P.B, C. Gabler, G.J. Killian, and A. Miyamoto. 2003. Tumor Necrosis Factor -alpha in the Bovine Oviduct during the Estrous Cycle: mRNA Expression and Affect on Secretion of Prostaglandins, Endothelin-1 and Angiotensin II. Biol. Reprod. (In Press). Willenburg, K.L., G.M. Miller, S.L. Rodriuguez-Zas, and R.V. Knox. 2003. Effect of boar exposure at time of insemination on factors influencing fertility in gilts. J. Anim. Sci. 81:9-15. Willenburg, K.L., G.M. Miller, S.L. Rodriuguez-Zas, and R.V. Knox. 2003. Influence of hormone supplementation to extended semen on AI, uterine contractions, establishment of a sperm reservoir, and fertility in swine. J. Anim. Sci. 81:821-829. Wise, T., D.D. Lunstra, G.A. Rohrer, and J.J. Ford. 2003. Relationships of testicular iron and ferritin concentrations with testicular weight and sperm production in boars. J. Anim. Sci. 81:503-511. Wu, G., J. Mao, L. Lai, T.C. McCauley, J. N. Caamaqo, K.W. Park, A.R. Rieke, T.C. Cantley, M.F. Smith, R.S. Prather, B.A. Didion, and B.N. Day. 2002. Magnesium inhibits in vitro fertilization of pig oocytes. Biol. Reprod. 66:311. Wu, G., Q.Y. Sun, J. Mao, L. Lai, T.C. McCauley, K.W. Park, R.S. Prather, B.A. Didion, B.N. Day. 2002. High developmental competence of pig oocytes after meiotic inhibition with a specific M-phase promoting factor kinase inhibitor, butyrolactone 1. Biol. Reprod. 67:170-177. Yang, W., J.R. Diehl, M. Yerle, J.J. Ford, R.K. Christenson, W.E. Roudebush, and W.E. Plummer. 2003. Chromosomal location, structure, and temporal expression of the platelet-activating factor (PAFr) gene in porcine endometrium and embryos relative to estrogen receptor  gene expression. Mol. Reprod. Dev. 64:4-12. Youngs, C. 2002. 2002 IETS foundation student research competition. Embryo Transfer Newsletter 20(1):4. Zhu, J., E. E. Telfer, J. Fletcher, A. Springbett, J. Dobrinsky, P. DeSousa, and I. Wilmut. 2002. Improvement of electrical activation protocol for porcine oocytes. Biol. Reprod. 66:635-41. Technical Reports: Anderson, L.L. 2002. Brain neuropeptides that control prolactin secretion cattle. Beef Research Report 2002. A.S. Leaflet R-1786. Anderson, L.L. 2002. Central nervous system regulation of pregnancy, parturition and lactation in cattle. Beef Research Report 2002. A.S. Leaflet R-1787. Anderson, L. L. and J. S. Lee. 2002. Nanoscale dynamics of growth hormone secretion in the pig. Swine Research Report - 2002. A.S. Leaflet R-1805. Guthrie, H.D. Handling affects fertility of frozen semen. National Hog Farmer. December 2002 Research Issue. Kim, K.S., N.T. Nguyen, J.M. Reecy, L.L. Anderson, and M.F. Rothschild. 2002. Molecular genetic studies of porcine genes for obesity. Swine Research Report - 2002. A.S. Leaflet R-1803. Youngs, C.R. 2002. The magnificent seven hormones controlling reproduction in the cow (Part II). Iowa Jersey Today 8(1):5-6. Youngs, C.R. 2002. Synchronization of estrus in dairy cattle using prostaglandin F2a (Part I). Iowa Jersey Today 8(2):10. Youngs, C.R. 2002. Synchronization of estrus in dairy cattle using prostaglandin F2a (Part II). Iowa Jersey Today 8(3):9-10. Zuelke, K. A. and L. Blomberg. Genetic Links to Embryonic Mortality Sought. National Hog Farmer. December 2002 Research Issue. Abstracts: Anderson.L., A. Glavaski, K. Jeftinija, C.G. Scanes and S. Jeftinija. 2002. Effect of ghrelin on calcium transient in isolated porcine pituitary cells. 32nd Annual Meeting of the Society for Neuroscience, Proceedings of Society for Neuroscience p. 27. Belton, R., and D.J. Miller. 2003. Cholesterol-dependent regulation of signaling in sperm. 6th Annual Meeting of the Society for the Study of Reproduction. Blomberg L., E.L. Long, T.S. Sonstegard, C.P. Van Tassel, J.R. Dobrinsky, and K.A. Zuelke. 2003. Serial analysis of gene expression (SAGE) during elongation of peri-implantation porcine embryos. 2003 Annual Conference of the Experimental Biology Society, San Diego, CA. LB151. Brad, A.M., C.L. Bormann, J.E. Swain, R.E. Durkin, A.E. Johnson, A.L. Clifford and R.L. Krisher. 2002. Glutathione content of in vivo- and in vitro- matured porcine oocytes. Theriogenology 57:715. Braundmeier, A.G., R.D. Shanks, D.J. Miller. 2002. The relationship of porcine sperm zona binding ability to fertility. Annual Meeting of the Society for the Study of Reproduction. Baltimore, MD. Burkin, H., and D.J. Miller. 2002. Expression of CASK in the mammalian sperm acrosome and processing during epididymal maturation. 42nd Annual Mtg of the Amer Soc of Cell Biol. Cederberg, R.A., B.E. Bass, C.F. Toombs, and B.R. White. 2002. Transcriptional regulation of the porcine GnRH receptor (GnRHR) gene. Biol. Reprod. 66(Suppl. 1):243. Cederberg, R.A., B.E. Bass, C.F. Toombs, and B.R. White. 2002. Transcriptional regulation of the porcine GnRH receptor (GnRHR) gene. Proceedings of the Nebraska-Iowa Physiological Society Joint Meeting, Creighton University, Omaha, Nebraska. Clapper, J.A. 2003. Anesthetic suppression of LH concentrations in barrows. J. Anim. Sci. 81 (Suppl. 1):246. Diekman, M.A., B. Delks and R. Allrich. 2003. Retention of non-traditional agriculture students in animal sciences. Midwest Animal Science Meetings, Des Moines, IA. Abstract 352, pg. 87. Durkin, R.E., J.E. Swain, A.L. Clifford and R.L. Krisher. 2002. Development of a sequential medium for in vitro culture of porcine embryos. Theriogenology 57:517. Echternkamp, S.E., R.M. Thallman, S.M. Kappes, and K.E. Gregory. Increased twinning and cow productivity in beef cattle selected for ovulation and twinning rate. 2002. Proceedings of the 7th World Congress on Genetics Applied to Livestock Production, Montpellier, France. Session 08, Reproduction; Communication No. 08-12. Enwall, L.E., G.C. Ostermeier, R.S. Bergfelt, L.N. Laplante, A. Kaya and J.J. Parrish. 2002. The effects of scrotal insulation on sperm morphology and in vitro fertilization. Biol. Reprod. 66(Suppl 1):164. Guthrie, H.D., G.R. Welch, and B.S. Cooper. 2002. Effects of cryopreservation and cooling on plasma membrane lipid disorder and integrity in boar spermatozoa. Biol Reprod. 66 (Suppl. 1):169. Guthrie, H.D., G.R. Welch, and J.R. Critser. 2002. Effects of osmotic stress and bovine serum albumin on sperm motion characteristics and plasma membrane integrity in boars. J. Anim Sci. 80 (Suppl. 1):202. Hilleson-Gayne, C., and J.A. Clapper. 2003. Use of an aromatase inhibitor to decrease serum concentrations of estradiol in boars. Accepted for presentation at The Society for Study of Reproduction Meetings, Cincinnati, OH., July 18-22. Kayser, J.P., P.J. Chenoweth, D.L. Davis, D.M. Grieger, J.C. Samper, and M.J. Arns. 2002. The effects of uterine cell-spermatozoa co-culture on the interactions between de-novo secreted and synthesized uterine proteins and stallion spermatozoa. Theriogenology 58:363-366. Kim, K.-S., L.L. Anderson, J.M. Reecy, N.T. Nguyen, G.S. Plastow, and M.F. Rothschild. 2002. Molecular genetic studies of porcine genes for obesity. Proceedings of International Symposium on Animal Genetics, Sao Palo, Brazil (In Press). Kim, K.-S., D.C. Ciobanu, L.L. Anderson, J.A. Reecy, W.H. Hsu, G.S. Plastow and M.F. Rothschild. 2002. Molecular genetics of porcine genes for obesity and diabetes. 84th Annual Meeting of The Endocrine Society. Endocrinology 143(Suppl. 1):400. Kim, K.-S., L.L. Anderson, C.K. Tuggle and M.F. Rothschild. 2002. Mapping and functional analysis of the porcine ghrelin gene A candidate for growth and appetite traits. Proceedings of the Plant & Animal Genome X (San Diego). Koch, J.W., K.N Livingston, S.R. Tatman, D. Alberts, D.M. Stocco, C.C. Chase, Jr., R.D. Randel, and T.H. Welsh, Jr. 2001. Comparison of adrenal and testis content of the steroidogenic acute regulatory (StAR) and P450 side-chain cleavage enzyme proteins in Angus, Brahman and Romosinuano bulls. J. Anim. Sci. 79(Suppl.1):229-230 (Abstract). Medicetty, S., A.R. Bledsoe, C.B. Fahrenholtz, D.L. Troyer, K.E. Mitchell, D.L. Davis, and M.L. Weiss. 2003. Transplantation of Pig Stem Cells into Rat Brain. Proceedings KCALSI Conference, p. 110. Parrish, J.J. and J.L. Susko-Parrish. 2002. A web-based reproduction laboratory: effect of web design on student use and learning. Biol. Reprod. 66(Suppl 1):180. Strauch, T.A., C.A. Abbey, J.W. Koch, S.R. Tatman, C.C. Chase, Jr., R.D. Randel, T.H. and Welsh, Jr. 2002. Expression of mRNA for growth hormone receptor, IGF-I, FSH-receptor, and LH-receptor in testis tissue of Angus, Brahman and Romosinuano bulls. J. Anim. Sci. 80(Suppl. 2):19. Stroble, K.A., J.R. Herrick, M.L. Conover and R.L. Krisher. 2002. Assessment of a novel media system for in vitro porcine embryo production. Biol. Reprod. 66 (Suppl. 1):145. Stroble, K.A., T.S. Stewart and R.L. Krisher. 2003. Length of cyropreservation has no effect on fertilizing ability of boar spermatozoa. Theriogenology 59:212. Zuelke, K.A. 2002. Serial analysis of gene expression (SAGE) in preimplantation stage swine embryos. Proceedings of the First International Meeting on Mammalian Embryo Genomics, Quebec City, Canada, July 20. Zuelke, K. A., L. Blomberg, E. L. Long, and T. S. Sonstegard. 2003. Serial analysis of gene expression (SAGE) between day 11 and day 12 porcine embryos. Theriogenology 59:435.

Impact Statements

Research investigations under the objectives of the committee will result in identification of complex biological processes involved in reproduction and lead to novel approaches in production agriculture.
The Seasonal Infertility in Swine Symposium has promoted collaborative efforts among research scientists, extension educators and pork industry representatives.
Dissemination of information from the Seasonal Infertility in Swine Symposium will foster communication between scientists and producers, resulting in improved reproductive management practices and increased profitability.
Publications representing collaborations among committee members will help advance our understanding of reproductive mechanisms in livestock, promoting increased reproductive efficiency and enhanced sustainability of domestic food animal production.

Date of Annual Report: 07/15/2004

Report Information

Annual Meeting Dates: 05/26/2004 - 05/27/2004
Period the Report Covers: 05/01/2003 - 05/01/2004

Participants

Miller, David - djmiller@uiuc.edu - U. of Illinois
;Diekman, Mark - mdiekman@purdue.edu - Purdue University
;Anderson, Lloyd - llanders@iastate.edu - Iowa State University
;Davis, Duane - ddavis@oznet.ksu.edu - Kansas State University
;Kirkwood, Roy - kirkwood@cvm.msu.edu - Michigan State University
;Tim Safranski, Tim - safranskit@missouri.edu - University of Missouri
;White, Brett - bwhite2@unl.edu - University of Nebraska
;Levis, Don - levis.7@osu.edu - Ohio State University
;Welsh, Tom - twelsh@cvm.tamu.edu - Texas A&M University
;Guthrie, Dave - dave@anri.barc.usda.gov - USDA/ARS/BARC/ Beltsville, MD
;Ford, Joe - ford@email.marc.usda.gov - USDA/ARS/USMARC, Clay Center, NE
;Parrish, John - parrish@ansci.wisc.edu - University of Wisconsin
;John Baker - baker@cvm.msu.edu - Michigan State University
;Hamernik, Deb - dhamerik@csrees.usda.gov - USDA/CSREES/PAS

Brief Summary of Minutes

Guest: Steve Kerns - International Boar Semen, Eldora, IA; Dr. Ron Christenson - USDA/ARS/USMARC; Dr. Harold Hodson, Gary Kuper, Michelle Wearmouth, Heidi Timmer and Kotaro Akachi, Swine Genetics, Inc., Cambridge, IA; Dr. Maynard Hogberg, Dr. Curt Youngs, Dr. Carolyn Komar, Dr. Collin Scanes, Kelly Wilhelms, Jin-Sook Lee and Marianna Moreira - Iowa State University; Tom Gall - M2 P2, Oakville, IA; Ben Bass - University of Nebraska; Kyle Lovercamp - University of Missouri

The annual meeting of the NCR-57 Regional Committee on Swine Reproduction was held in Room 1204, the Ensminger Room, of Kildee Hall on the Iowa State University campus. Associate Dean Hoiberg welcomed the group to Iowa State University and provided a summary of programs in the Agricultural Experiment Station.

Administrative advisor, Dr. Baker, reported that the renewal submission for NCR-57 went well and the renewal was approved for 2004-2009. The primary request was to change the name of the committee to Swine Reproductive Physiology.

Dr. Deb Hamernik, USDA/CSREES/PAS advisor, distributed a handout that summarized pertinent issues including recently hired CSREES staff, openings and upcoming retirements.

Station reports were presented in the mornings of May 26 and 27.

On the afternoon of May 26, a workshop on Boar Physiology began with a welcome from Dr. Maynard Hogberg, Chair of the Department of Animal Science who provided a brief summary of the four research sections within the department, meats, animal breeding, physiology and nutrition. Steve Kerns of International Boar Semen of Eldora, IA, identified eight issues in which additional information would aid the use of artificial insemination by U.S. swine producers. Dr. Ron Christenson presented a summary prepared from material provided by Dr. Billy Flowers of North Carolina State University on the effect of stressors on sperm morphology. Dr. Don Levis provided a challenging overview of the status of information on nutritional requirements of boars. Little current information exists that was obtained in today‘‘s boars with reduced fatness. This void evolved in an environment where great emphasis was and continues to be placed on genetic change associated with growth rate, backfat thickness and muscling. Dr. John Parrish summarized the status of information relating to fertility of sperm produced by bull studs and how this should impact research relating to boar studs. Dr. David Guthrie reviewed the current status of use of frozen boar sperm. Cryo-preserved boar sperm remain viable for a shorter time within the uterus than extended sperm. Optimal time for insemination with cryo-perserved boar sperm is four hours before to the time of ovulation.

During the business meeting, an invitation from John Parrish for the committee to meet in Madison, WI in 2005, tentatively May 25 and 26, 2005, was accepted.

Discussion of the boar physiology workshop addressed the desire to achieve greater interaction with extension educators. In an effort to accomplished this goal, the 2006 workshop of NCR-57 is tentatively planned for North Carolina to be in closer proximity to extension educators in that region of the U.S. Incoming chair, Brett White, was requested to discuss this option with Billy Flowers. Dave Guthrie agreed to contact Billy Flowers to develop a topic for the workshop.

Members decided that NCR-57 should communicate to Deb Hamernik and Mark Mirando, via letter, a priority for an NRI integrated program that relates more to the interest of NCR-57 committee members. David Miller, Duane Davis and Tim Safranski volunteered to take the lead on this effort. It is vital to have this information to Mark Mirando by May 15th.

Minutes Attachment:

Attachment

Accomplishments

1. The proposal to renew NCR-57 was completed, submitted and approved with the name changed to Swine Reproductive Physiology. 2. A second workshop was organized and conducted with the goal of developing a CD for distribution to extension educators who have responsibility for swine reproduction programs. This workshop addressed Boar Physiology to compliment the first workshop that addressed Seasonal Infertility followed by distribution of a CD of these presentations. 3. Scientists who participate in NCR-57 exchange research findings and develop collaborative projects to identify mechanisms and genes that control pubertal development, resumption of estrous cycles after parturition, folliculogenesis, gametogenesis, fertilization, pregnancy recognition and maintenance, and cryopreservation of gametes. 4. Publications produced by members of the committee and their associates during this past year are listed on the web page under Publications. 5. Members continued to examine teaching and advising procedures that are practiced at each participating university to enhance the quality of these services to students. Plans for the coming year: 1. Plan for a third workshop to be held in 2006 with the goal of developing greater interactions with extension educators. A committee was established to select the topic and to present a proposal at the 2005 committee meeting. 2. During 2004, expand development of the NCR-57 web page. A committee was appointed to capture the history of the committee. Review of the web page will be a component of the 2005 meeting. 3. Committee members will continue collaborative efforts to investigate mechanisms that control reproduction efficiency of livestock species with emphasis on swine.

Publications

Manuscripts: Albin DM, J.E. Wubben, J.M. Rowlett, K.A. Tappenden, R.A. Nowak. 2004. Small intestinal nutrient transport and barrier function in two porcine breeds respond differently to acute immune challenge. Submitted to J of Physiology Anderson, L.L. 2004. Invited MINIREVIEW titled: Discovery of a new cellular structure: The Porosome, Elucidation of the molecular mechanism of secretion. CELL BIOLOGY INTERNATIONAL 28:3-5. Anderson, L.L. and B.P. Jena. 2004. Invited chapter entitled, Fusion pores in growth hormone secreting cells of the pituitary gland: An AFM study. In: AFM IN BIOLOGY AND MEDICINE. J. Wiley & Sons, Inc., New York. (In press). Anderson, L.L., S. Jeftinija and C.G. Scanes. 2004. Invited MINIREVIEW titled: Growth hormone secretion: Molecular and cellular mechanisms and in vivo approaches. EXPERIMENTAL BIOLOGY AND MEDICINE 229:291-302. Bahr J.M., M. Nakai, A. Rivera, J. Walsh, G.L. Evans, S. Lotinum, R.T. Turner, M. Black, E.H. Jeffery. 2004. Dietary soy protein and isoflavones: Minimal beneficial effects on bone and no effect on the reproduction tract of sexually mature ovariectomized Sprague-Dawley rats. Menopause (in press) Bazer F.W., T.S. Spencer and R.D. Geisert. 2004. Anatomy and Physiology of the Female Reproductive Tract. In: Encylopedia of Animal Science, W.G. Pond and A.W. Bell, Eds, Marcel Dekker, Inc., New York. (In Press). Belstra, B.A., Flowers, W.L., and See, M.T. 2004. Factors affecting temporal relationships between estrus and ovulation in commercial sow farms. Ani. Reprod. Sci. (in press). Blomberg, L. and K. A. Zuelke. 2004. Serial gene expression (SAGE) during porcine embryo development. Reprod. Fert. Dev. 16:87-92. Boltz D., N. Nakai, J.M. Bahr. 2004. Avian infectious bronchitis virus: A possible cause of reduced fertility in the rooster. Avian Diseases (submitted) Boone W.R., B.B. Keck, J.C. Catlin, K.J. Casey, E.T. Boone, P.S. Dye, R.J. Schuett, T. Tsubota, J.M. Bahr. 2004. Evidence that bears are induced ovulators. Theriogenology 61:1163-1169 Braundmeier A.G., J.M. Demers, R.D. Shanks, and D.J. Miller. 2004. The ability of porcine sperm to bind the zona pellucida is related to litter size after insemination. J. Anim. Sci. 82:452-458 Braundmeier A.G., W.G. Breed, and D.J. Miller. 2004. 1,4-Galactosyltransferase on sperm from a marsupial, the brushtail possum, Trichosurus vulpecula. Reproduction (Submitted) Burke, C. R., M.L. Mussard, C.L. Gasser, D.E. Grum, M.L. Day. 2003. Estradiol benzoate delays new follicular wave emergence in a dose dependent manner after ablation of the dominant ovarian follicle in cattle. Theriogenology, 60:647-658. Burkin H.R., A.P. Alves-Vieira, and D.J. Miller. 2004. Localization of zona pellucida receptors on live sperm by fluorophore-conjugated solubilized zona pellucida proteins. Meth. Molecular Biol.: Germ Cell Protocols 253:79-93 Burkin H.R., L. Zhao, and D.J. Miller. 2004. CASK is in the mammalian sperm head and is processed during epididymal maturation. Mol. Reprod. Dev. (In Press) Burkin H.R., L. Zhao, K. Reim, N. Brose, and D.J. Miller. 2004. Complexin 1 is required for mammalian sperm acrosomal exocytosis. Dev. Biol. (Submitted) Chen L, S. Brown, M. Nakai, R.A. Nowak. 2004. Expression and hormonal regulation of extracellular matrix metalloproteinase inducer (EMMPRIN) during mouse embryo implantation. Submitted to Biol. Reprod. Clamon L.A., R.A. Nelson, L.M. Pyter, J.M. Bahr. 2003. Induction of pseudopregnancy in the American Black Bear (Ursus americanus). J. Exp. Zool. Part A Comp. Exp. Biol. 298:162-166 Clark, S. and R. Knox. 2003. Efficiency of boar semen production. Embryo Transfer Newsletter. 21:4-10 Corbin, C.J., F.M. Moran, J.D. Vidal, J.J. Ford, T. Wise, S.M. Mapes, V.C. Njar, A.M. Brodie, and A.J. Conley. 2003. Biochemical assessment of limits to estrogen synthesis in porcine follicles. Biol. Reprod. 69:390-397. Corbin, C.J., S.M. Mapes, J. Marcos, C.H. Shackleton, D. Morrow, S. Safe, T Wise, J.J. Ford, and A.J. Conley. 2004. Paralogues of porcine aromatase cytochrome P450: A novel hydroxylase activity is associated with the survival of a duplicated gene. Endocrinology 145:2157-2164. Creech, B.L., Spears, J.W., Flowers, W.L., Hill, G.M., Lloyd, K.E., and Armstrong, T.A. 2004. Effect of trace mineral level and source (inorganic vs. chelated) on performance, mineral status and fecal mineral excretion in pigs from weaning through finishing. J. Anim. Sci. (in press) Crisp, S.E.R.H., J.B. Griffin, B.R. White, C.F. Toombs, G. Camporeale, H.M. Said, and J. Zempleni. 2004. Biotin supply affects rates of cell proliferation, biotinylation of carboxylases and histones, and expression of the gene encoding the sodium-dependent multivitamin transporter in JAr choriocarcinoma cells. Eur. J. Nutr. 43:23-31. Daberkow, R.L., B.R. White, R.A. Cederberg, J.B. Griffin and J. Zempleni. 2003. Monocarboxylate transporter 1 mediates biotin uptake in human peripheral blood mononuclear cells. J. Nutr. 133:2703-2706. Davis, D.L. and Stevenson, J.S. 2003. Synchronization of Estrus: Horses, Pigs, Sheep, and Goats. A. W. Bell and W. G. Pond (Ed.) Encyclopedia of Animal Science, Marcel Dekker Inc., New York. Day, M. L. 2004. Hormonal induction of estrous cycles in anestrous, Bos taurus beef cows. ICAR Meetings, In Press. Day, M. L. and D. E. Grum. 2003. Estrous synchronization in heifers. Ann. Conf. of the Soc. for Theriogenology: 145-154. Day, M. L. 2004. Hormonal induction of estrous cycles in anestrous, Bos taurus beef cows. Anim. Reprod. Sci. (In Press). Dobrinsky, J. R. 2004. Advancements in cryopreservation of domestic animal embryos. Theriogenology. (In Press). Dunn, J.D., B.J. Johnson, J.P. Kayser, A.T. Waylan, E.K. Sissom, and J.S. Drouillard. 2003. Effects of flax supplementation and a combined trenbolone acetate and estradiol implant on circulating insulin-like growth factor-I (IGF-I) and muscle IGF-I messenger RNA levels in beef cattle. J. Anim. Sci. 81:3028-3034. Echternkamp, S.E., A.J. Roberts, D.D. Lunstra, T. Wise, and L.J. Spicer. 2004. Ovarian follicular development in cattle selected for twin ovulations and births. J. Anim. Sci. 82:459-471. Ellsworth, B.S.*, B.R. White*, A.T. Burns, B.D. Cherrington, A.M. Otis, and C.M. Clay. 2003. c-Jun N-terminal kinase (JNK) activation of activator protein-1 underlies homologous regulation of the gonadotropin-releasing hormone receptor gene in T3-1 cells. Endocrinology 144:839-849. *Both authors contributed equally to this work. Ferrell A.D., J.R. Malayer, K.L. Carraway and R.D. Geisert. 2003. Sialomucin complex (Muc4) expression in porcine endometrium during the oestrous cycle and early pregnancy. Reprod. Dom. Anim. 38:1-3. Flowers, W.L. 2004. Biotechnology: Artificial Insemination. 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Impact Statements

Disseminated information from the Boar Physiology Workshop to extension educators to foster communication between scientists and swine producers.
Published collaborative efforts among committee members to advance understanding of reproductive mechanisms in livestock, to promote greater reproductive efficiency and to enhance sustainability of domestic food animal production.
Conducted research within the objectives of the committee to identify complex biological processes involved in reproduction that will lead to improved technology for animal production.
The workshop on Seasonal Infertility in Swine enhanced debate among committee members and fostered a recommendation to USDA, CSREES, NRI to list seasonal infertility in swine as a priority area for Integrated Programs within the NRI, Animal Reproduction funding program.