Annual/Termination Reports:

[11/25/2008] [09/20/2010] [03/20/2011] [07/03/2012]

Report Information

Participants

Regina Redman University of Washington, Seattle;
Rusty Rodriguez US Geological Survey & University of Washington, Seattle;
Jenny Lorang Oregon State University;
Jeff Rollins University of Florida;
Marilyn J. Roossinck, Noble Foundation, Oklahoma;
Nancy Keller University of Wisconsin;
JinWoo Bok University of Wisconsin;
Saori Amaike University of Wisconsin;
Graeme Garvey University of Wisconsin;
Clarissa Booth University of Wisconsin;
Mona Shabaan University of Wisconsin;
Tika Adhikari North Dakota State University;
Tim Friesen USDA-ARS Red River Agricultural Research Center;
Frances Trail Michigan State University;
Jim Kerns University of Wisconsin;
Moyi Li University of Florida;
Brett Williams Texas A&M University;
Mehdi Kabbage Texas A&M University;
Tom Mitchell Ohio State University;

Brief Summary of Minutes

Please see attached file for NCCC207's complete report.

Accomplishments

Publications

Impact Statements

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Report Information

Participants

Brief Summary of Minutes

Please see attached file for meeting minutes.

Accomplishments

Publications

Impact Statements

Back to top

Report Information

Participants

Brief Summary of Minutes

Accomplishments

Publications

Impact Statements

Back to top

Report Information

Participants

Regina Redman; University of Washington; redmanr@uw.edu
Rusty Rodriguez University of Washington; rustyrod@u.washington.edu
Dan Ebbole; Texas A&M University; d-ebbole@tamu.edu
Heather Wilkinson; Texas A&M University;h-wilkinson@tamu.edu
Martin Dickman; Texas A&M University; mbdickman@tamu.edu
Tim Friesen; USDA-ARS North Dakota State University;timothy.friesen@ars.usda.gov
Zhaohui Liu; North Dakota State University; zhh.lin@ndsu.edu
Jeffrey Rollins; University of Florida; rollinsj@ufl.edu
Michael Kolomiets; Texas A&M University; kolomiets@tamu.edu
Mehdi Kabbage; Texas A&M University; pcd@tamu.edu
Marilyn J. Roossinck; Pennsylvania State University; mjr25@psu.edu
Ben Doughan; University of Florida; bdoughan@ufl.edu
Xiaofei Liang; University of Florida; xiaofeiliang@ufl.edu
Jessie Fernandez; University of Nebraska; jfernandez99@huskers.unl.edu
Richard A Wilson; University of Nebraska; rwilson10@unl.edu

Brief Summary of Minutes

Accomplishments

1.Research projects were coordinated among NCCC-207 participants during the annual meeting resulting in new collaborations and standardization of protocols.<br /> <br /> 2.Postdoctoral and PhD graduate students were mentored and professional development enhanced through the scientific exchange provided by this meeting.<br /> <br /> 3.Research results were published in peer-reviewed journals by members of the committee including several joint, collaborative publications.<br />

Publications

Abeysekara, N.S., J.D. Faris, S. Chao, P.E. McClean, and T.L. Friesen. 2012. Whole-Genome QTL Analysis of Stagonospora nodorum Blotch Resistance and Validation of the SnTox4-Snn4 Interaction in Hexaploid Wheat. Phytopathology. 102:94-104. <br /> <br /> Adhikari, T.B., E.W. Jackson, S. Gurung, J.M. Hansen, and J.M. Bonman. 2011. Association Mapping of Quantitative Resistance to Phaeosphaeria nodorum in Spring Wheat Landraces from the USDA National Small Grains Collection. Phytopathology. 101:1301-1310. <br /> <br /> Adhikari, T.B., S. Gurung, J.M. Hansen, and J.M. Bonman. 2012. Pathogenic and Genetic Diversity of Xanthomonas translucens pv. undulosa in North Dakota. Phytopathology. 102:390-402. <br /> <br /> Allen, A., E. Islamovic, J. Kaur, S. Gold, D. Shah, and T.J. Smith. 2011. Transgenic maize plants expressing the Totivirus antifungal protein, KP4, are highly resistant to corn smut. Plant Biotechnol. J. 9:857-864. <br /> <br /> Amselem, J., C.A. Cuomo, J.A.L. van Kan, M. Viaud, E.P. Benito, A. Couloux, P.M. Coutinho, R.P. de Vries, P.S. Dyer, S. Fillinger, E. Fournier, L. Gout, M. Hahn, L. Kohn, N. Lapalu, K.M. Plummer, J.-M. Pradier, E. Quevillon, A. Sharon, A. Simon, A. ten Have, B. Tudzynski, P. Tudzynski, P. Wincker, M. Andrew, V. Anthouard, R.E. Beever, R. Beffa, I. Benoit, O. Bouzid, B. Brault, Z. Chen, M. Choquer, J. Collemare, P. Cotton, E.G. Danchin, C. Da Silva, A. Gautier, C. Giraud, T. Giraud, C. Gonzalez, S. Grossetete, U. Gueldener, B. Henrissat, B.J. Howlett, C. Kodira, M. Kretschmer, A. Lappartient, M. Leroch, C. Levis, E. Mauceli, C. Neuveglise, B. Oeser, M. Pearson, J. Poulain, N. Poussereau, H. Quesneville, C. Rascle, J. Schumacher, B. Segurens, A. Sexton, E. Silva, C. Sirven, D.M. Soanes, N.J. Talbot, M. Templeton, C. Yandava, O. Yarden, Q. Zeng, J.A. Rollins, M.-H. Lebrun, and M. Dickman. 2011. Genomic Analysis of the Necrotrophic Fungal Pathogens Sclerotinia sclerotiorum and Botrytis cinerea. PLoS Genet. 7. <br /> <br /> <br /> Bar-Dror, T., M. Dermastia, A. Kladnik, M.T. Znidaric, M.P. Novak, S. Meir, S. Burd, S. Philosoph-Hadas, N. Ori, L. Sonego, M.B. Dickman, and A. Lers. 2011. Programmed Cell Death Occurs Asymmetrically during Abscission in Tomato. Plant Cell. 23:4146-4163. <br /> <br /> Chacko, N., and S. Gold. 2012. Deletion of the Ustilago maydis ortholog of the Aspergillus sporulation regulator medA affects mating and virulence through pheromone response. Fungal genetics and biology/: FG & B. 49. <br /> <br /> Chu, C., Z. Niu, S. Zhong, S. Chao, T.L. Friesen, S. Halley, E.M. Elias, Y. Dong, J.D. Faris, and S.S. Xu. 2011. Identification and molecular mapping of two QTLs with major effects for resistance to Fusarium head blight in wheat. Theor. Appl. Genet. 123:11071119. <br /> <br /> Dickman, M.B., and P. de Figueiredo. 2011. Comparative Pathobiology of Fungal Pathogens of Plants and Animals. PLoS Pathog. 7. <br /> <br /> Faris, J.D., Z. Zhang, J.B. Rasmussen, and T.L. Friesen. 2011. Variable Expression of the Stagonospora nodorum Effector SnToxA Among Isolates Is Correlated with Levels of Disease in Wheat. Mol. Plant-Microbe Interact. 24:1419-1426. <br /> <br /> Feldman, T.S., M.R. Morsy, and M.J. Roossinck. 2012. Are communities of microbial symbionts more diverse than communities of macrobial hosts? Fungal Biol. 116:465-477. <br /> <br /> Fernandez, J., and R.A. Wilson. 2011. The sugar sensor, trehalose-6-phosphate synthase (Tps1), regulates primary and secondary metabolism during infection by the rice blast fungus: will Magnaporthe oryzae's sweet tooth become its Achilles' heel? Mycology - An International Journal on Fungal Biology. 2. <br /> <br /> Fernandez, J., J.D. Wright, D. Hartline, C.F. Quispe, N. Madayiputhiya, and R.A. Wilson. 2012. Principles of carbon catabolite repression in the rice blast fungus: Tps1, Nmr1-3, and a MATE-family pump regulate glucose metabolism during infection. PLoS genetics. 8. <br /> <br /> Franceschetti, M., E. Bueno, R.A. Wilson, S.L. Tucker, C. Gomez-Mena, G. Calder, and A. Sesma. 2011. Fungal Virulence and Development Is Regulated by Alternative Pre-mRNA 3 End Processing in Magnaporthe oryzae. PLoS Pathog. 7. <br /> <br /> Friesen, T.L., and J.D. Faris. 2012. Characterization of plant-fungal interactions involving necrotrophic effector-producing plant pathogens. Methods in molecular biology (Clifton, N.J.). 835. <br /> <br /> Ghaffary, S.M.T., J.D. Faris, T.L. Friesen, R.G.F. Visser, T.A.J. van der Lee, O. Robert, and G.H.J. Kema. 2012. New broad-spectrum resistance to septoria tritici blotch derived from synthetic hexaploid wheat. Theor. Appl. Genet. 124:125-142. <br /> <br /> Giordano, P.R., T.A. Nikolai, R. Hammerschmidt, and J.M. Vargas. 2012. Timing and Frequency Effects of Lightweight Rolling on Dollar Spot Disease in Creeping Bentgrass Putting Greens. Crop Sci. 52:1371-1378. <br /> <br /> Gurung, S., S.B. Goodwin, M. Kabbage, W.W. Bockus, and T.B. Adhikari. 2011. Genetic Differentiation at Microsatellite Loci Among Populations of Mycosphaerella graminicola from California, Indiana, Kansas, and North Dakota. Phytopathology. 101:1251-1259. <br /> <br /> Henry, A., S. Chopra, D.G. Clark, and J.P. Lynch. 2012. Responses to low phosphorus in high and low foliar anthocyanin coleus (Solenostemon scutellarioides) and maize (Zea mays). Funct. Plant Biol. 39:255-265. <br /> <br /> Hu, J.N., C.X. Chen, T. Peever, H. Dang, C. Lawrence, and T. Mitchell. 2012. Genomic characterization of the conditionally dispensable chromosome in Alternaria arborescens provides evidence for horizontal gene transfer. BMC Genomics. 13. <br /> <br /> Jonkers, W., A.E. Rodriguez Estrada, K. Lee, A. Breakspear, G. May, and H.C. Kistler. 2012. Metabolome and Transcriptome of the Interaction between Ustilago maydis and Fusarium verticillioides In Vitro. Appl. Environ. Microbiol. 78:3656-3667. <br /> <br /> Kim, H., C. Chen, M. Kabbage, and M.B. Dickman. 2011. Identification and Characterization of Sclerotinia sclerotiorum NADPH Oxidases. Appl. Environ. Microbiol. 77:7721-7729. <br /> <br /> Klindworth, D.L., Z. Niu, S. Chao, T.L. Friesen, Y. Jin, J.D. Faris, X. Cai, and S.S. Xu. 2012. Introgression and characterization of a goatgrass gene for a high level of resistance to ug99 stem rust in tetraploid wheat. G3 (Bethesda, Md.). 2. <br /> <br /> Kong, L.-A., J. Yang, G.-T. Li, L.-L. Qi, Y.-J. Zhang, C.-F. Wang, W.-S. Zhao, J.-R. Xu, and Y.-L. Peng. 2012. Different Chitin Synthase Genes Are Required for Various Developmental and Plant Infection Processes in the Rice Blast Fungus Magnaporthe oryzae. PLoS Pathog. 8. <br /> <br /> Kour, A., K. Greer, B. Valent, M.J. Orbach, and C. Soderlund. 2012. MGOS: Development of a Community Annotation Database for Magnaporthe oryzae. Mol. Plant-Microbe Interact. 25:271-278. <br /> <br /> Li, M., X. Liang, and J.A. Rollins. 2012. Sclerotinia sclerotiorum gamma-Glutamyl Transpeptidase (Ss-Ggt1) Is Required for Regulating Glutathione Accumulation and Development of Sclerotia and Compound Appressoria. Mol. Plant-Microbe Interact. 25:412-420. <br /> <br /> Liberti, D., J.A. Rollins, and P.F. Harmon. 2012. Evidence for Morphological, Vegetative, Genetic, and Mating-Type Diversity in Sclerotinia homoeocarpa. Phytopathology. 102:506-518. <br /> <br /> Lim, F.Y., Y. Hou, Y. Chen, J.-H. Oh, I. Lee, T.S. Bugni, and N.P. Keller. 2012. Genome-Based Cluster Deletion Reveals an Endocrocin Biosynthetic Pathway in Aspergillus fumigatus. Appl. Environ. Microbiol. 78:4117-4125. <br /> <br /> Liu, Z., and T.L. Friesen. 2012. Polyethylene glycol (PEG)-mediated transformation in filamentous fungal pathogens. Methods in molecular biology (Clifton, N.J.). 835. <br /> <br /> Liu, Z., Z. Zhang, J.D. Faris, R.P. Oliver, R. Syme, M.C. McDonald, B.A. McDonald, P.S. Solomon, S. Lu, W.L. Shelver, S. Xu, and T.L. Friesen. 2012. The Cysteine Rich Necrotrophic Effector SnTox1 Produced by Stagonospora nodorum Triggers Susceptibility of Wheat Lines Harboring Snn1. PLoS Pathog. 8. <br /> <br /> Mahto, B.N., S. Gurung, A. Nepal, and T.B. Adhikari. 2012. Morphological, pathological and genetic variations among isolates of Cochliobolus sativus from Nepal. Eur. J. Plant Pathol. 133:405-417. <br /> <br /> Mahto, B.N., S. Gurung, and T.B. Adhikari. 2011. Assessing genetic resistance to spot blotch, Stagonospora nodorum blotch and tan spot in wheat from Nepal. Eur. J. Plant Pathol. 131:249260. <br /> <br /> Okada, R., E. Kiyota, S. Sabanadzovic, H. Moriyama, T. Fukuhara, P. Saha, M.J. Roossinck, A. Severin, and R.A. Valverde. 2011. Bell pepper endornavirus: molecular and biological properties, and occurrence in the genus Capsicum. J. Gen. Virol. 92:2664-2673. <br /> <br /> Patel, J.S., N.C. Gudmestad, S. Meinhardt, and T.B. Adhikari. 2012. Pyraclostrobin sensitivity of baseline and fungicide exposed isolates of Pyrenophora tritici-repentis. Crop Prot. 34:37-41. <br /> <br /> Patel, J.S., S.W. Meinhardt, H. Sierotzki, G. Stammler, N.C. Gudmestad, and T.B. Adhikari. 2011. A Two-Step Molecular Detection Method for Pyrenophora tritici-repentis Isolates Insensitive to Qol Fungicides. PLANT DIS. 95:1558-1564. <br /> <br /> Paul, J.-Y., D.K. Becker, M.B. Dickman, R.M. Harding, H.K. Khanna, and J.L. Dale. 2011. Apoptosis-related genes confer resistance to Fusarium wilt in transgenic Lady Finger bananas. Plant Biotechnol. J. 9:1141-1148. <br /> <br /> Rodriguez-Kessler, M., L. Baeza-Montanez, M.D. Garcia-Pedrajas, A. Tapia-Moreno, S. Gold, J.F. Jimenez-Bremont, and J. Ruiz-Herrera. 2012. Isolation of UmRrm75, a gene involved in dimorphism and virulence of Ustilago maydis. Microbiol. Res. 167:270-282. <br /> <br /> Roossinck, M.J., S. Sabanadzovic, R. Okada, and R.A. Valverde. 2011. The remarkable evolutionary history of endornaviruses. J. Gen. Virol. 92:2674-2678. <br /> <br /> Sanchez, J.F., A.D. Somoza, N.P. Keller, and C.C.C. Wang. 2012. Advances in Aspergillus secondary metabolite research in the post-genomic era. Nat. Prod. Rep. 29:351371. <br /> <br /> Scherm, B., M. Orru, V. Balmas, F. Spanu, E. Azara, G. Delogu, T.M. Hammond, N.P. Keller, and Q. Migheli. 2011. Altered trichothecene biosynthesis in TRI6-silenced transformants of Fusarium culmorum influences the severity of crown and foot rot on durum wheat seedlings. Mol. Plant Pathol. 12:759-771. <br /> <br /> Soukup, A.A., J. Strauss, and N.P. Keller. 2012. Regulation of Aspergillus nidulans secondary metabolite gene clusters through histone acetylation. Biochem. Cell Biol. 90:112-112. <br /> <br /> Stobbe, A.H., U. Melcher, M.W. Palmer, M.J. Roossinck, and G. Shen. 2012. Co-divergence and host-switching in the evolution of tobamoviruses. J. Gen. Virol. 93:408-418. <br /> <br /> Tan, K.-C., M. Ferguson-Hunt, K. Rybak, O.D.C. Waters, W.A. Stanley, C.S. Bond, E.H. Stukenbrock, T.L. Friesen, J.D. Faris, B.A. McDonald, and R.P. Oliver. 2012. Quantitative Variation in Effector Activity of ToxA Isoforms from Stagonospora nodorum and Pyrenophora tritici-repentis. Mol. Plant-Microbe Interact. 25:515-522. <br /> <br /> Thapa, V., U. Melcher, G.B. Wiley, A. Doust, M.W. Palmer, K. Roewe, B.A. Roe, G. Shen, M.J. Roossinck, Y.M. Wang, and N. Kamath. 2012. Detection of members of the Secoviridae in the Tallgrass Prairie Preserve, Osage County, Oklahoma, USA. Virus research. 167. <br /> <br /> van Doorn, W.G., E.P. Beers, J.L. Dangl, V.E. Franklin-Tong, P. Gallois, I. Hara-Nishimura, A.M. Jones, M. Kawai-Yamada, E. Lam, J. Mundy, L. a. J. Mur, M. Petersen, A. Smertenko, M. Taliansky, F. Van Breusegem, T. Wolpert, E. Woltering, B. Zhivotovsky, and P.V. Bozhkov. 2011. Morphological classification of plant cell deaths. Cell Death Differ. 18:12411246.<br /> <br /> <br /> Varbanova, M., K. Porter, F. Lu, J. Ralph, R. Hammerschmidt, A.D. Jones, and B. Day. 2011. Molecular and Biochemical Basis for Stress-Induced Accumulation of Free and Bound p-Coumaraldehyde in Cucumber. Plant Physiol. 157:1056-1066. <br /> <br /> Vartapetian, A.B., A.I. Tuzhikov, N.V. Chichkova, M. Taliansky, and T.J. Wolpert. 2011. A plant alternative to animal caspases: subtilisin-like proteases. Cell Death Differ. 18:1289-1297. <br /> <br /> Vartapetian, A.B., A.I. Tuzhikov, N.V. Chichkova, M. Taliansky, and T.J. Wolpert. 2011. A plant alternative to animal caspases: subtilisin-like proteases. Cell Death Differ. 18:1289-1297. <br /> <br /> Veluchamy, S., B. Williams, K. Kim, and M.B. Dickman. 2012. The CuZn superoxide dismutase from Sclerotinia sclerotiorum is involved with oxidative stress tolerance, virulence, and oxalate production. Physiol. Mol. Plant Pathol. 78:1423. <br /> <br /> Vidigal Filho, P.S., M.C. Goncalves-Vidigal, A.B. da Rocha, R. Hammerschmidt, W.W. Kirk, J.P. Poletine, and J.D. Kelly. 2011. Characterization and content of total soluble protein and amino acids of traditional common bean cultivars collected in Parana state, Brazil. J. Food Agric. Environ. 9:143147. <br /> <br /> Vincent, D., L.A. Du Fall, A. Livk, U. Mathesius, R.J. Lipscombe, R.P. Oliver, T.L. Friesen, and P.S. Solomon. 2012. A functional genomics approach to dissect the mode of action of the Stagonospora nodorum effector protein SnToxA in wheat. Mol. Plant Pathol. 13:467-482. <br /> <br /> Wang, C., S. Zhang, R. Hou, Z. Zhao, Q. Zheng, Q. Xu, D. Zheng, G. Wang, H. Liu, X. Gao, J.-W. Ma, H.C. Kistler, Z. Kang, and J.-R. Xu. 2011. Functional Analysis of the Kinome of the Wheat Scab Fungus Fusarium graminearum. PLoS Pathog. 7. <br /> <br /> Wang, G., C. Wang, R. Hou, X. Zhou, G. Li, S. Zhang, and J.-R. Xu. 2012. The AMT1 Arginine Methyltransferase Gene Is Important for Plant Infection and Normal Hyphal Growth in Fusarium graminearum. PloS one. 7. <br /> <br /> Yin, W.-B., S. Amaike, D.J. Wohlbach, A.P. Gasch, Y.-M. Chiang, C.C.C. Wang, J.W. Bok, M. Rohlfs, and N.P. Keller. 2012. An Aspergillus nidulans bZIP response pathway hardwired for defensive secondary metabolism operates through aflR. Mol. Microbiol. 83:1024-1034. <br /> <br /> <br />

Impact Statements

1. Members have been instrumental in sequencing and analyzing genomes of more than ten important fungal plant pathogens. Most recently, a high impact publication concerning genome annotation and comparative genomic was published for Sclerotinina sclerotiorum. The comparative analysis of this genome has revealed novel determinants of fungal virulence. Targeted approaches to interfere with fungal infection and colonization of hosts have been suggested and have the potential for deducing multi-millions of dollars in crop loss if successfully implemented.
2. NCCC207 members have generated understandings of plant-fungal interactions in terms of host susceptibility, pathogen virulence mechanisms and fungal-plant interactions on multiple parasitic (mutualism to pathogenic) levels. The scale of these investigations range from the single organism to the ecosystem and from single genes to genomes. These activities have altered strategies of disease management to increase productivity and lower environmental impact.
3. Significant advancements in the genetic mapping of quantitative disease resistance traits have been made for two major diseases of wheat, Fusarium Head Blight and Stagonospora nodorum Blotch. In addition transgenic crop engineering has demonstrated the effectiveness of anti-fungal protein expression in resistance to corn smut disease. These findings are being applied for crop improvement to improve food security and mitigate production losses.

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