SAES-422 Multistate Research Activity Accomplishments Report
Sections
Status: Approved
Basic Information
- Project No. and Title: W102 : Integrated Methods of Parasite Control for Improved Livestock Production
- Period Covered: 07/01/2001 to 06/01/2002
- Date of Report: 09/25/2003
- Annual Meeting Dates: 07/18/2002 to 07/19/2002
Participants
Lou Gasbarre, ARS-USDA, Beltsville, MD; John Stuedemann, ARS-USDA,Watkinsville, GA; Bert Stromberg, University of Minnesota; Ed Platzer, University of California, Riverside; Ray Kaplan, University of Georgia, Athens; Milt McAllister, University of Illinois, Urbana; Bob Ridley, Kansas State University, Manhattan; Tom Klei, Jim Miller, Louisiana State University, Baton Rouge; Anne Zajac, Virginia Polytechnic Institute and State University, Blacksburg; Bonner Stewart (former member, Baton Rouge, LA); Guests; Mark Spire, Kansas State University, Manhattan; Dwight Bowman, Cornell University; Cliff Monahan, Ohio State University; Mike Jenkins, ARS-USDA Watkinsville GA.
[Minutes]
Accomplishments
Evaluation of the anthelmintic activity of naturally occurring fungi in laboratory and field experiments. Work is still being done in several species. Ray Kaplan (GA) and Jim Miller (LA) reported that a commercial producta bolus or feed additive-- is still under development. Jim Miller indicated that this product would probably not be effective alone for parasite control but would be useful in an integrated parasite control program. The product appears to have considerable potential. Additional stations may evaluate this product.
Numerous stations have collaborated on the use of diatomaceous earth as an anthelmintic, a compound that is very popular among organic producers. Zajac (VA), Miller (LA) and Stromberg (MN) have found that there is little if any activity against helminths in sheep or cattle. A joint publication should be developed.
Studies that identify parasitic antigens that can serve as targets for control of parasitic infections are progressing. These studies are collaborative between (Gasbarre, Zarlenga; ARS MD, Lindsey; VA)
Pasture management as a means of controlling parasitic infections is also an ongoing project between (Kaplan, GA; Studeman, ARS GA; Stromberg, MN)
A collaborative study on the development of resistance to equine anthelmintics was discussed. The protocols have been developed and the study will be conducted next year, collaborating stations are (GA, LA, FL).
Numerous stations have collaborated on the use of diatomaceous earth as an anthelmintic, a compound that is very popular among organic producers. Zajac (VA), Miller (LA) and Stromberg (MN) have found that there is little if any activity against helminths in sheep or cattle. A joint publication should be developed.
Studies that identify parasitic antigens that can serve as targets for control of parasitic infections are progressing. These studies are collaborative between (Gasbarre, Zarlenga; ARS MD, Lindsey; VA)
Pasture management as a means of controlling parasitic infections is also an ongoing project between (Kaplan, GA; Studeman, ARS GA; Stromberg, MN)
A collaborative study on the development of resistance to equine anthelmintics was discussed. The protocols have been developed and the study will be conducted next year, collaborating stations are (GA, LA, FL).
Impacts
- Diatomaceous earth does not appear to have any activity as an anthelmintic.
- Identification of antigens that can serve as targets for control of parasitic infections.
- Numerous lay publications dicussing parasite control.
Publications
Peer Reviewed Journals
Elliott, D.E., Urban, J.F., Jr., Argo, C.K., Weinstock, J.V. Does the failure to acquire helminthic parasites predispose to Crohn‘s disease? The Federation of American Societies for Experimental Biology Journal. 2000. v. 14. p. 1848-1855.
Finkelman, F.D., Urban, J.F. The other side of the coin: The protective role of the type 2 (Th2) cytokines. Journal of Allergy and Clinical Immunology. 2001. v. 107. p. 772-780.
Gasbarre, L.C., Leighton, E.A., Sonstegard, T. Role of the bovine immune system and genome in resistance to gastrointestinal nematodes. Veterinary Parasitology. 2001. v. 98. p. 51-64.
Hediger-Weithaler, B.M., Heussler, V.T., Eicher, R., Zakher, A., Zarlenga, D.S., Gasbarre, L.C., Waldvogel, A.S. Interferon- and interleukin 4 mRNA expression by PBMCs from BVDV seropositive pregnant and non-pregnant cattle. Veterinary Immunology and Immunopathology. 2000. v. 77. p. 201-212.
Johnson, P., Keen, N.T., Lunney, J.K., Sadowsky, M.J. Conference Editorial: Agricultural Microbes Genome 2. Comparative and Functional Genomics. 2001. v. 2. p. 10-13. [http://www3.interscience.wiley.com/cgi- bin/issuetoc?ID=77002017]
Kaplan, R.M. 2001. Liver Flukes in Cattle: A Review of the Economic Impact and Considerations for Control. Veterinary Therapeutics,2(1): 40-50.
La Rosa, G., Marucci, G., Zarlenga, D.S., Pozio, E. Trichinella pseudospiralis populations from the Palearctic region and their relationship with populations of Nearctic and Australian regions. International Journal of Parasitology. 2001. v. 31. p. 297-305.
Rosypal AC, Lindsay DS, Duncan R Jr., Ahmed A, Zajac A, Dubey J. 2001. Mice lacking the gene for inducible or endothelial nitric oxide are resistant to sporocyst induced Sarcocystis neurona Infections. Vet Parasit 103:315-321
Solano-Aguilar, G.I., Beshah, E., Vengroski, K., Zarlenga, D., Jauregui, L., Cosio, M., Douglass, L.W., Dubey, J.P., Lunney, J.K. Cytokine and lymphocyte profile in miniature swine after oral infection with Toxoplasma gondii oocysts. International Journal of Parasitology. 2001. v. 31. p. 187-195.
Tarigo-Martinie, J.L,, A.R. Wyatt, AND R.M. Kaplan. 2001. Prevalence And Clinical Implications of Anthelmintic Resistance In Cyathostomes of Horses JAVMA. 218(12): 1957-1960.
Terrill, T.H., Kaplan, R.M., Larsen, M., Samoles, O.M., Miller, J.E., Gelaye, S. 2001. Anthelmintic Resistance on Goat Farms in Georgia Efficacy of Anthelmintics against Gastrointestinal Nematodes in Two Selected Goat Herds. Veterinary Parasitology. 97: 261-268
Zarlenga, D.S., Chute, M.B., Gasbarre, L.C., Boyd, P. A multiplex PCR assay for differentiating economically important gastrointestinal nematodes of cattle. Veterinary Parasitology. 2001. v. 97. p. 199-209.
Lay Press Publications
Gasbarre, L. ,Agricultural Research,; "Infectious Emergencies; Creating the Tools to Study Immune Responses in Farm Animals. June 2001. p. 18-19.
Gasbarre, L.W., Beef Magazine,"The double trouble of parasites. April 2001. p. 22.
Kaplan, RM, Beware of parasite drug resistance, Sheep Connection, Vol. 3, No. 1, January 2001.
Kaplan, RM, Sheep resistant worms off of your farm Sheep Connection, Vol. 3, No. 2, April 2001.
Kaplan, R.M. "Control of Gastrointestinal Helminths in Cattle", in Proceedings of the 72nd Annual Convention of the Florida Veterinary Medical Association, Wyndham Palace Resort and Spa, Orlando Florida, September 7-9, 2001.
Kaplan, R.M. 2001. Advances in Parasite Control, Supplement to Veterinary Practice News, Equine Practice, pp. 11-13.
Kaplan, RM, Emerging Issues in Small Ruminant Parasite Control, in Proceedings of the Georgia Veterinary Medical Association Food Animal Conference, Tifton Bull Test Station, Tifton Georgia, March 9 - 10, 2002.
Kaplan,R.M., J.A. STUEDEMANN, A.J. FRANZLUEBBERS, AND D.H. SEMAN, The Salem Road Study: Restoration of Degraded Land with Pasture --The Concept of Parasite-Free Pastures. in Proceedings of the 57th Southern Pasture and Forage Crop Improvement Conference, University of Georgia, Athens, Georgia, April 23-25, 2002
Abstracts
Gasbarre, L.C., Stout, W.L., Lohr, L., Guerrero, J. Gastrointestinal nematodes on a grazing dairy operation: Effect of parasite control on production and parasite transmission. Proceedings of the American Association of Veterinary Parasitology. 2001. p. 51.
Henson, E, and Kaplan, RM, Characterization of b-Tubulin Genes from Equine Cyathostome Populations with Differing Sensitivities to Benzimidazole Anthelmintics. (poster) University of Georgia, College of Veterinary Medicine Research Day, Athens, GA, February 13, 2001.
Kaplan, RM and Howell, SB, An Improved Method for Counting and Identifying Nematode Larvae in the Drenchrite Larval Development Assay. 18th annual meeting of the Southern Conference on Animal Parasites, University of Florida, Gainesville, FL, February 10-11, 2001.
Kaplan, RM and Ware, L, Efficacy of moxidectin against an ivermectin-resistant isolate of Haemonchus contortus in goats. 46th Annual meeting of the American Association of Veterinary Parasitologists, Boston, MA, July 14-17, 2001.
Kaplan, R.M. , L.L. Mortensen, L.H. Williamson , T.H. Terrill, R. Kircher, AND M. Larsen. Prevalence of anthelmintic resistance in gastrointestinal nematodes of goats in the southern United States. Novel Approaches III Conference, Moredun Research Institute, Edinburgh, Scotland, July 2-5, 2002.
Kaplan R.M., TR KLEI, ET LYONS, DD FRENCH, GD LESTER, AND CH COURTNEY. Prevalence of Anthelmintic Resistance on Horse Farms in the Southern United States. 47th Annual meeting of the American Association of Veterinary Parasitologists, Nashville, TN, July 13-16, 2002.
Kaplan, R.M., MORTENSEN, L.H. WILLIAMSON , T.H. TERRILL, R. KIRCHER, AND M. LARSEN. Prevalence of anthelmintic resistance in gastrointestinal nematodes of goats. 47th Annual meeting of the American Association of Veterinary Parasitologists, Nashville, TN, July 13-16, 2002.
Kim, I and Kaplan, RM, Mechanisms of avermectin resistance in parasitic nematodes: PCR cloning of the UNC-9 gene homologue from Cylicocyclus nassatus (Cyathostominae). (poster) University of Georgia, Center for Undergraduate Research Opportunities Symposium, Athens, GA, February 26-28, 2001.
Kim, I and Kaplan, RM, Mechanisms of avermectin resistance in parasitic nematodes: PCR cloning of the UNC-9 gene homologue from Cylicocyclus nassatus (Cyathostominae). (poster) University of Georgia, College of Veterinary Medicine Research Day, Athens, GA, February 13, 2001.
Kringel, H., Dubey, J.P., Beshah, E., Hecker, R., Urban, J.F. Effect of CpG oligodeoxynucleotide (ODN)-induced immune activation of porcine immunity to Toxoplasma gondii. American Association of Veterinary Parasitology. 2001. v. 46. p. 15.
Morimoto, M., Zarlenga, D., Beard, H., Mat thews, B., Urban, J.F. cDNA microarray analysis of 4th stage larvae (L4) of Ascaris suum. The Federation of American Societies for Experimental Biology Journal. 2001. v. 15 .p. A6 91 .
Rosypal, AC, Lindsay DS, Duncan RB, Ahmed, A, Zajac, AM, Dubey, JP. 2001. Sporocyst induced Sarcocystis neurona infections inmice lacking the gene for inducible nitric oxide. 46th Annual meetting of the American Society of Veterinary Parasitologists. Boston, Massachusetts.
Solano-Aguilar, G.I., Beshah, E., Vengroski, K., Zarlenga, D., Jauregui, L., Cosio, M., Douglass, L.W., Dubey, J.P., Lunney, J.K. Cytokine and lymphocyte profile after oral infection with Toxoplasma gondii oocysts. Scandinavian Journal of Immunology. 2001. v. 54. p. 93.
Tandon, V., R.M. KAPLAN. In vitro larval development assay (DrenchRite) for the detection of anthelmintic resistance in horses. 47th Annual meeting of the American Association of Veterinary Parasitologists, Nashville, TN, July 13-16, 2002.
Urban, J.F., Schopf, L., Madden, K.B., Nobern-Trauth, N., Finkelman, F.D. Nematode biology determines the requirements for expression of IL-4 receptor alpha chain on lymphoid and/or non-lymphoid cells that regulate intestinal expulsion. The Federation of American Societies for Experimental Biology Journal. 2001. v. 15. p. A690.
Vanimisetti, H. B., S. P. Greiner, A. M. Zajac, and D. R. Notter. 2002.
Between-breed variation in response to Haemonchus contortus infection in sheep. J. Anim. Sci. 80(Suppl. 1)
Ware, L and Kaplan, RM, Moxidectin Selection of an Ivermectin-Resistant Strain of Haemonchus contortus in Goats. 18th annual meeting of the Southern Conference on Animal Parasites, University of Florida, Gainesville, FL, February 10-11, 2001.
Ware, L and Kaplan, RM, Moxidectin Selection of an Ivermectin-Resistant Strain of Haemonchus contortus in Goats. (poster) University of Georgia, College of Veterinary Medicine Research Day, Athens, GA, February 13, 2001.
Zajac A, Wildeus S, Duncan R. 2001. Parasite resistance in an improved West Indian hair sheep breed. 18th International Conference of the World Association for the Advancement of Veterinary Parasitology. Stresa, Italy
Elliott, D.E., Urban, J.F., Jr., Argo, C.K., Weinstock, J.V. Does the failure to acquire helminthic parasites predispose to Crohn‘s disease? The Federation of American Societies for Experimental Biology Journal. 2000. v. 14. p. 1848-1855.
Finkelman, F.D., Urban, J.F. The other side of the coin: The protective role of the type 2 (Th2) cytokines. Journal of Allergy and Clinical Immunology. 2001. v. 107. p. 772-780.
Gasbarre, L.C., Leighton, E.A., Sonstegard, T. Role of the bovine immune system and genome in resistance to gastrointestinal nematodes. Veterinary Parasitology. 2001. v. 98. p. 51-64.
Hediger-Weithaler, B.M., Heussler, V.T., Eicher, R., Zakher, A., Zarlenga, D.S., Gasbarre, L.C., Waldvogel, A.S. Interferon- and interleukin 4 mRNA expression by PBMCs from BVDV seropositive pregnant and non-pregnant cattle. Veterinary Immunology and Immunopathology. 2000. v. 77. p. 201-212.
Johnson, P., Keen, N.T., Lunney, J.K., Sadowsky, M.J. Conference Editorial: Agricultural Microbes Genome 2. Comparative and Functional Genomics. 2001. v. 2. p. 10-13. [http://www3.interscience.wiley.com/cgi- bin/issuetoc?ID=77002017]
Kaplan, R.M. 2001. Liver Flukes in Cattle: A Review of the Economic Impact and Considerations for Control. Veterinary Therapeutics,2(1): 40-50.
La Rosa, G., Marucci, G., Zarlenga, D.S., Pozio, E. Trichinella pseudospiralis populations from the Palearctic region and their relationship with populations of Nearctic and Australian regions. International Journal of Parasitology. 2001. v. 31. p. 297-305.
Rosypal AC, Lindsay DS, Duncan R Jr., Ahmed A, Zajac A, Dubey J. 2001. Mice lacking the gene for inducible or endothelial nitric oxide are resistant to sporocyst induced Sarcocystis neurona Infections. Vet Parasit 103:315-321
Solano-Aguilar, G.I., Beshah, E., Vengroski, K., Zarlenga, D., Jauregui, L., Cosio, M., Douglass, L.W., Dubey, J.P., Lunney, J.K. Cytokine and lymphocyte profile in miniature swine after oral infection with Toxoplasma gondii oocysts. International Journal of Parasitology. 2001. v. 31. p. 187-195.
Tarigo-Martinie, J.L,, A.R. Wyatt, AND R.M. Kaplan. 2001. Prevalence And Clinical Implications of Anthelmintic Resistance In Cyathostomes of Horses JAVMA. 218(12): 1957-1960.
Terrill, T.H., Kaplan, R.M., Larsen, M., Samoles, O.M., Miller, J.E., Gelaye, S. 2001. Anthelmintic Resistance on Goat Farms in Georgia Efficacy of Anthelmintics against Gastrointestinal Nematodes in Two Selected Goat Herds. Veterinary Parasitology. 97: 261-268
Zarlenga, D.S., Chute, M.B., Gasbarre, L.C., Boyd, P. A multiplex PCR assay for differentiating economically important gastrointestinal nematodes of cattle. Veterinary Parasitology. 2001. v. 97. p. 199-209.
Lay Press Publications
Gasbarre, L. ,Agricultural Research,; "Infectious Emergencies; Creating the Tools to Study Immune Responses in Farm Animals. June 2001. p. 18-19.
Gasbarre, L.W., Beef Magazine,"The double trouble of parasites. April 2001. p. 22.
Kaplan, RM, Beware of parasite drug resistance, Sheep Connection, Vol. 3, No. 1, January 2001.
Kaplan, RM, Sheep resistant worms off of your farm Sheep Connection, Vol. 3, No. 2, April 2001.
Kaplan, R.M. "Control of Gastrointestinal Helminths in Cattle", in Proceedings of the 72nd Annual Convention of the Florida Veterinary Medical Association, Wyndham Palace Resort and Spa, Orlando Florida, September 7-9, 2001.
Kaplan, R.M. 2001. Advances in Parasite Control, Supplement to Veterinary Practice News, Equine Practice, pp. 11-13.
Kaplan, RM, Emerging Issues in Small Ruminant Parasite Control, in Proceedings of the Georgia Veterinary Medical Association Food Animal Conference, Tifton Bull Test Station, Tifton Georgia, March 9 - 10, 2002.
Kaplan,R.M., J.A. STUEDEMANN, A.J. FRANZLUEBBERS, AND D.H. SEMAN, The Salem Road Study: Restoration of Degraded Land with Pasture --The Concept of Parasite-Free Pastures. in Proceedings of the 57th Southern Pasture and Forage Crop Improvement Conference, University of Georgia, Athens, Georgia, April 23-25, 2002
Abstracts
Gasbarre, L.C., Stout, W.L., Lohr, L., Guerrero, J. Gastrointestinal nematodes on a grazing dairy operation: Effect of parasite control on production and parasite transmission. Proceedings of the American Association of Veterinary Parasitology. 2001. p. 51.
Henson, E, and Kaplan, RM, Characterization of b-Tubulin Genes from Equine Cyathostome Populations with Differing Sensitivities to Benzimidazole Anthelmintics. (poster) University of Georgia, College of Veterinary Medicine Research Day, Athens, GA, February 13, 2001.
Kaplan, RM and Howell, SB, An Improved Method for Counting and Identifying Nematode Larvae in the Drenchrite Larval Development Assay. 18th annual meeting of the Southern Conference on Animal Parasites, University of Florida, Gainesville, FL, February 10-11, 2001.
Kaplan, RM and Ware, L, Efficacy of moxidectin against an ivermectin-resistant isolate of Haemonchus contortus in goats. 46th Annual meeting of the American Association of Veterinary Parasitologists, Boston, MA, July 14-17, 2001.
Kaplan, R.M. , L.L. Mortensen, L.H. Williamson , T.H. Terrill, R. Kircher, AND M. Larsen. Prevalence of anthelmintic resistance in gastrointestinal nematodes of goats in the southern United States. Novel Approaches III Conference, Moredun Research Institute, Edinburgh, Scotland, July 2-5, 2002.
Kaplan R.M., TR KLEI, ET LYONS, DD FRENCH, GD LESTER, AND CH COURTNEY. Prevalence of Anthelmintic Resistance on Horse Farms in the Southern United States. 47th Annual meeting of the American Association of Veterinary Parasitologists, Nashville, TN, July 13-16, 2002.
Kaplan, R.M., MORTENSEN, L.H. WILLIAMSON , T.H. TERRILL, R. KIRCHER, AND M. LARSEN. Prevalence of anthelmintic resistance in gastrointestinal nematodes of goats. 47th Annual meeting of the American Association of Veterinary Parasitologists, Nashville, TN, July 13-16, 2002.
Kim, I and Kaplan, RM, Mechanisms of avermectin resistance in parasitic nematodes: PCR cloning of the UNC-9 gene homologue from Cylicocyclus nassatus (Cyathostominae). (poster) University of Georgia, Center for Undergraduate Research Opportunities Symposium, Athens, GA, February 26-28, 2001.
Kim, I and Kaplan, RM, Mechanisms of avermectin resistance in parasitic nematodes: PCR cloning of the UNC-9 gene homologue from Cylicocyclus nassatus (Cyathostominae). (poster) University of Georgia, College of Veterinary Medicine Research Day, Athens, GA, February 13, 2001.
Kringel, H., Dubey, J.P., Beshah, E., Hecker, R., Urban, J.F. Effect of CpG oligodeoxynucleotide (ODN)-induced immune activation of porcine immunity to Toxoplasma gondii. American Association of Veterinary Parasitology. 2001. v. 46. p. 15.
Morimoto, M., Zarlenga, D., Beard, H., Mat thews, B., Urban, J.F. cDNA microarray analysis of 4th stage larvae (L4) of Ascaris suum. The Federation of American Societies for Experimental Biology Journal. 2001. v. 15 .p. A6 91 .
Rosypal, AC, Lindsay DS, Duncan RB, Ahmed, A, Zajac, AM, Dubey, JP. 2001. Sporocyst induced Sarcocystis neurona infections inmice lacking the gene for inducible nitric oxide. 46th Annual meetting of the American Society of Veterinary Parasitologists. Boston, Massachusetts.
Solano-Aguilar, G.I., Beshah, E., Vengroski, K., Zarlenga, D., Jauregui, L., Cosio, M., Douglass, L.W., Dubey, J.P., Lunney, J.K. Cytokine and lymphocyte profile after oral infection with Toxoplasma gondii oocysts. Scandinavian Journal of Immunology. 2001. v. 54. p. 93.
Tandon, V., R.M. KAPLAN. In vitro larval development assay (DrenchRite) for the detection of anthelmintic resistance in horses. 47th Annual meeting of the American Association of Veterinary Parasitologists, Nashville, TN, July 13-16, 2002.
Urban, J.F., Schopf, L., Madden, K.B., Nobern-Trauth, N., Finkelman, F.D. Nematode biology determines the requirements for expression of IL-4 receptor alpha chain on lymphoid and/or non-lymphoid cells that regulate intestinal expulsion. The Federation of American Societies for Experimental Biology Journal. 2001. v. 15. p. A690.
Vanimisetti, H. B., S. P. Greiner, A. M. Zajac, and D. R. Notter. 2002.
Between-breed variation in response to Haemonchus contortus infection in sheep. J. Anim. Sci. 80(Suppl. 1)
Ware, L and Kaplan, RM, Moxidectin Selection of an Ivermectin-Resistant Strain of Haemonchus contortus in Goats. 18th annual meeting of the Southern Conference on Animal Parasites, University of Florida, Gainesville, FL, February 10-11, 2001.
Ware, L and Kaplan, RM, Moxidectin Selection of an Ivermectin-Resistant Strain of Haemonchus contortus in Goats. (poster) University of Georgia, College of Veterinary Medicine Research Day, Athens, GA, February 13, 2001.
Zajac A, Wildeus S, Duncan R. 2001. Parasite resistance in an improved West Indian hair sheep breed. 18th International Conference of the World Association for the Advancement of Veterinary Parasitology. Stresa, Italy