SAES-422 Multistate Research Activity Accomplishments Report

Status: Approved

Basic Information

Participants

Kenneth G.D. Allen (Colorado State University); Jennifer Anderson (Colorado State University); Jay Whelan (University of Tennessee); Nancy K. Lewis (University of Nebraska); Audrey Adler (Rutgers University); Debra Palmer Keenan (Rutgers University); Kevin Fritsche (University of Missouri); Barbara Lohse (Kansas State University); Richard Baybutt (Kansas State University); Kate Claycombe (Michigan State University); Robert Chapkin (Texas A & M); K. Shane Broughton (University of Wyoming); Doreen Woodward (Administrative Adviser; Michigan State University), Susan Welch (CSREES Representative, Washington, DC). Members Absent: Rosemary Wander (University of North Carolina, Greensboro); Daniel Gallaher (University of Minnesota)

The meeting was called to order by the chair, K. Shane Broughton, at 1:15 pm, November 4, 2004 at the Kellogg Hotel & Conference Center, East Lansing, MI. Kevin Fritsche served as secretary for this meeting. Introductions & Opening Remarks: The meeting opened with a welcome from John Baker, Acting Director of Michigan Ag. Expt. Station. He gave a brief overview of the areas of focus for the MAES: Food & Health, Environmental Stewardship, Food, Family & Fiber, Microbial Food Safety, Food Security. We were introduced to our new USDA rep., Susan Welch. She told about her background as Natl. Program Leader for Nutrition Education Leadership. She informed us that the USDA has two open positions and the staff has been working hard over the past few months with finalizing the Dietary Guidelines Advising Report. Also, she told us that the NRI competitive grants program may get a $20M boost from Congress next fiscal year. How much of that will go into either of the nutrition-related programs (i.e., 31.0 and 31.5) is unclear. This is the first year of the new policy at the NRI regarding funding larger grants (~$500,000) and for longer (4 yrrs). As a result, in the 31.0 program only 10 of 104 applications were funded with a budget that has remained at ~$4M for many years. The new program (31.5) area on nutrition and obesity (31.5) the budget is ~$8.5M. These projects are supposed to integrate basic science with education, outreach, and outcomes. Ten out of 89 applications were funded in this program area. Susan encouraged us to consider applying for a conference grant to support the activities of this Multi-state project. Doreen Woodward (Ad. Advisor, MSU) introduced herself and shared several helpful handouts regarding the NIMS reporting system and how to fill out the SAES-422 Annual Reports. She also reminded us that our mid-project report would be reviewed this Spring. Research Seminar: A research seminar entitled, Hepatic metabolism and its impact on fatty acid-regulated transcription factors was presented by Donald Jump (Prof. Physiol., Biochem. & Mol. Biol., MSU). The presentation was followed by many questions and a lively discussion. Announcements: 1. The FDA allowed Health Claims for food rich in EPA and DHA were reviewed and discussed. 2. IFT Symposium: Members were asked if there was interest in organizing another symposium on n-3 PUFA nutrition for the annual IFT meeting. Several members had previously (July 2003) participated in such an activity at the invitation of Bruce Watkins, an IFT and NC1167 member. No one expressed an interest in organizing another IFT symposium, in part, because no one present was an IFT member. 3. EB05 Symposium: N-3 PUFA, Transitioning Research to Education will be on April 5, 2005 in San Diego. The deadline for submitting symposia proposals for EB06 is January. It was decided that we should wait until after our upcoming n-3 PUFA symposium before we submit another proposal. However, there was a consensus that we should sponsor a mini-symposium topic that could bring together all the abstracts in the n-3 PUFA area. Kevin Fritsche agreed to pursue this with Mary Banks at the ASNS office and report back to the committee within a few weeks. 4. Society for Nutrition Education (SNE): Members of the NC1167 committee will be organizing a symposium at SNEs upcoming meeting in Orlando, FL (July 24-27). Jay Whelan was nominated and agreed to be the opening speaker. 5. Evidence Report (#89) from HHS on N-3 PUFA impact on a number of human health conditions including: type II diabetes, metabolic syndrome, IBD, arthritis, SLE, and osteoporosis. Copies of the Summary were distributed and discussed. All committee members were encouraged to go to the Web site indicated in the Summary (i.e., www.ahrq.gov) and read the full document. Nutrition Education Subcommittee Report: (Drs. Anderson, Lewis, Adler, Palmer-Keenan, Lohse) (Note: Dr. Lohse, assigned to NC1167 from the Kansas Agricultural Experiment Station, moved to Pennsylvania State University in March, 2004, and will continue to be active in this committee.) From October 1, 2003 to September 30, 2004, the Nutrition Education unit of NC 1167 developed a survey for registered dietitians to assess n-3 fat knowledge, practices regarding n-3 fat advice with patients, and n-3 fat continuing education venue preferences. Surveys were administered using two procedures: self-report web-based and telephone interview with interviewers recording responses on a web survey modified from the self-report version. Kansas (Pennsylvania) participated in pilot testing the process, interviewee training, and database development. Kansas (Pennsylvania) took the lead in database development and management and developing and administering the web-based survey. For the web-based survey 350 RDs from four states were invited to participate, and 74 RDs completed the survey; for the telephone format, 116 from a subject pool of 346 RDs from the four states completed surveys. Findings from190 participating registered dietitians are being analyzed and manuscript preparation is underway. Steps to develop an n-3 fatty acid knowledge intervention have been discussed in conference calls and sponsorship as well as grant submission are under consideration. Findings revealed limited knowledge by registered dietitians, yet a strong interest in learning more and in providing information to consumers and other clients, especially using a web-based format or handouts and printed materials. For 2005, Pennsylvania will take the lead on data analysis of survey outcomes while New Jersey will lead analysis comparing survey administration modes. Colorado and Nebraska will take the lead on intervention development. Following this report the group as a whole discussed the strength of the evidence to support benefits for a long list of health conditions. We attempted to categorize each health condition * EPA&DHA linkage as either: strong (A), modest (B), and weak or inconclusive (C), none (D). The subcommittee agreed to email this listing for further consideration by the entire committee. The first day meeting was adjourned at 5:35 p.m. The second day meeting was called to order at 8:40 by the chair. Announcements: 1. Chair for next year will be Kevin Fritsche; secretary will be Kate Claycombe. (Kate understands that she will be the incoming chair for 06 as per our newly agreed upon line of succession.) 2. Next years meeting will held in Fort Collins, CO and be hosted by Ken Allen and Jennifer Anderson, reps. from Colorado State University. 3. NC1167 Listserv: Debra Palmer Keenan wanted to know if she should maintain the listserv for this group. She did so last year, yet no one seemed to use it. We all agreed that we should keep it and USE IT. All committee members are to send her an email from our email address that we want on the listserv. Station Reports: The following member states gave brief research reports: Tennessee, Missouri, Colorado (2), Michigan, Nebraska, and Texas. These reports elicited many questions and some lively discussion. More complete reports are attached to these minutes. Lunch & Guest Speaker: We worked through lunch and had an informative discussion with Gale Strasburg, acting chair of the Food Science & Human Nutr. Dept. at MSU, regarding the recent reorganization of Colleges at MSU and the ongoing search for a new chair. The meeting was adjourned at 1:45 pm.

Accomplishments

Collaborative efforts of the basic science groups and nutrition education component were discussed. The collaboration has continued to strengthened as the project progresses; the group is developing into a cohesive core of n3 fatty acid researchers. For the basic researchers most of the collaborative efforts to date have been conceptual (i.e., at the level of experimental design). Specifically, each investigator has incorporated two common elements into there currently funded research programs: to address both n-3 form and amount relative to potency in their research models. Investigations being done include a number of different experimental conditions with various biological outcomes, unique to each researcher. Plans were made to enhance the collaboration between the basic researchers. On behalf of several participants, Dr. Whelan (Tenn) is preparing an application to the USDA to underwrite that cost of generating common basal and experimental animal diets containing human-equivalent amounts of various n-3 fatty acids. Each researcher will conduct studies with these common research diets. Such an approach is novel and will greatly facilitate direct comparisons of the relative potency of various n-3 fatty acids using numerous biological read-outs related to human health issues. The nutrition education researchers participating in this regional project generated novel baseline data regarding n-3 fatty acid knowledge of dieticians in several member states. They are prepared to impliment an educational outreach program that they have designed to increase awareness of and knowledge in the nutritional benefits associated with increased n-3 fatty acid consumption by the general public and by specific target populations. Impacts to be measured in the future will include: (1) enhanced knowledge of n-3 fatty acids by dieticians; (2) Increased frequency that the dieticians talk to patients about n-3 fatty acids; (3) Evidence of increase intake of n-3 fatty acids in the populations served by these health professionals within the participating states The impact of this project will be to provide essential primary data in a number of different systems to answer two fundamental questions regarding the recent recommendations to increase dietary n-3 fatty acid intake for health maintenance and disease prevention: (1) Which form of n-3 fatty acid are most potent? (2) How much is needed to provide health benefits? In addition, the nutrition education component will inform nutrition professionals on these results for education of RDs and their clients.

Impacts

  1. Our data demonstrate that within human equivalent doses using long-chain n-3 fatty acids (DHA, EPA, SDA) is superior to a-linolenic acid.
  2. We discovered that most nutrition health professionals as a general rule do not know enough about n-3 fatty acids to feel comfortable giving advice to their clients.

Publications

Allen, K.G.D., Bristow, S.J. and Yu, L. Hypolipidemic effects of modified psyllium preparations. J. Agric. Food Chem. 52: 4998-5003, 2004. Irons, R., Anderson, M.J., Zhang, M. and Fritsche K.L. (2003) Dietary fish oil impairs primary host resistance against L. monocytogenes more than the immunological memory response. J. Nutr. 133: 1163-1169. Zhang, M. and Fritsche K.L. (2004) Fatty acid-mediated inhibition of interleukin-12 production by murine macrophages is independent of peroxisome proliferator-activated receptor-g. Brit. J. Nutr. 91: 733-739. Anderson M and Fritsche KL (2004) Dietary polyunsaturated fatty acids modulate in vivo, antigen-driven CD4+ T-cell proliferation in mice. J. Nutr. 134: 1978-1983. Anderson, M. (2004) Dietary omega-3 polyunsaturated fatty acids and Th1 immunity. Ph.D. Dissertation, University of Missouri, Columbia, MO. Irons, R. (2004) Fish oil severely impairs immunity to Listeria monocytogenes without affecting the adaptive immune response. Ph.D. Dissertation, University of Missouri, Columbia, MO. Harkins, JM, Moustaid-Moussa, N, Penner, KM, Pestka, JJ, Chung, YJ, North, CM, and Claycombe, KJ. Expression of interleukin-6 is greater in preadipocytes than in adipocytes of 3T3-L1 cells and C57BL/6J and ob/ob mice. J Nutr. 134:2673-2677, 2004. Harkins, JM, Whelan J, Jones L, Ken Allen, and Claycombe, KJ. Inhibitory effect of SDA and EPA on 3T3-L1 preadipocyte secretion of interleukin-6 (IL-6). In preparation, 2004. Sindelar, Carrie A., Lewis, Nancy M., Scheerger, Sarah B., Plugge, Sheri L., Eskridge, Kent M., and Wander, Rosemary C. 2004. Serum lipids of physically active adults consuming omega-3 fatty acid-enriched eggs or standard eggs. Nutrition Research 24:731-739. Heidal, Kimberly, Lewis, Nancy, and Evans, Sharon. 2004. Survey of omega-3 food selections in heart patients living in the Midwest. Nutrition Research 24:741-747. Heidal, Kimberly, Lewis, Nancy M. 2004. Omega-3 fatty acid nutrition education resources. Journal of Nutrition Education and Behavior 36 (4):209-210. Al-Numair, Khalid and Lewis, Nancy M. 2004. Omega-3 fatty acid intake and incidence of nonfatal myocardial infarction differ between coastal and internal regions of Saudi Arabia. Ecology of Food and Nutrition 43:93-106. Ciubotaru I, Lee YS, Wander. Dietary fish oil decrease C-reaction protein, interleukin-6, and triacylglycerol to HDL-cholesterol ratio in postmenopausal women on HRT. J Nutr Biochem. 2003 Sep;14(9):513-21. Toobert DJ, Glasgow RE, Strycker LA, Barrera M Jr, Radcliffe JL, Wander RC, Bagdade JD. Biologic and quality-of-life outcomes from the Mediterranean Lifestyle Program: a randomized clinical trial. Diabetes Care. 2003 Aug;26(8):2288-93. Hall JA, Tooley KA, Gradin JL, Jewell DE, Wander RC. Effects of dietary n-6 and n-3 fatty acids and vitamin E on the immune response of healthy geriatric dogs. Am J Vet Res. 2003 Jun;64(6):762-72. Manns PJ, Williams DP, Snow CM, Wander RC. Physical activity, body fat, and serum C-reactive protein in postmenopausal women with and without hormone replacement. Am J Hum Biol. 2003 Jan-Feb;15(1):91-100. Whelan, J. and McEntee, M.F. 2003. NSAIDs, Prostaglandins and APC-Driven Intestinal Tumorigenesis. In: Cyclooxygenase-2 Blockade in Cancer Prevention and Therapy (Ed., Harris, R.E.) Humana Press, Inc.. Totowa, NJ D.W.L. Ma, J. Seo, L.A. Davidson, E.S. Callaway, Y.Y. Fan, J.R. Lupton and R.S. Chapkin. n-3 PUFA alter caveolae lipid composition and resident protein localization in mouse colon. Faseb Journal, April 2004, 10.1096/fj.03-0604fje, 18:1040-1042, 2004. L.M. Sanders, C.E. Henderson, M.Y. Hong, R. Barhoumi, R.C. Burghardt, R.J. Carroll, N.D. Turner, R.S. Chapkin and J.R. Lupton. Pro-oxidant environment of the colon compared to the small intestine may contribute to greater cancer susceptibility. Cancer Letters 208:155-161, 2004. K.C. Switzer, Y-Y. Fan, N. Wang, D.M. McMurray and R.S. Chapkin. Dietary n-3 polyunsaturated fatty acids promote activation-induced cell death in the Th1-polarized murine CD4+ T-cells. Journal of Lipid Research, 45:1482-1492, 2004. L.A. Davidson, D.V. Nyuyen, R.M. Hokanson, E.S. Callaway, R.B. Isett, N.D. Turner, E.R. Dougherty, J.R. Lupton, R.J. Carroll and R.S. Chapkin. Chemopreventive n-3 polyunsaturated fatty acids reprogram genetic signatures during colon cancer initiation and progression in the rat. Cancer Research 64:6797-6804, 2004.
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