SAES-422 Multistate Research Activity Accomplishments Report

Sections

Status: Approved

Basic Information

Participants

Barefoot, Susan (sbrft@clemson.edu) - Clemson University; Blanton, John (john.blanton@ttu.edu)- Texas Tech University; Brashears, Mindy (mindy.brashears@ttu.edu) - Texas Tech University; Dickson, James S. (jdickson@iastate.edu)- Iowa State University; Goodridge, Lawrence (lgoodrid@uwyo.edu) - University of Wyoming; Janes, Marlene (mjanes@agctr.lsu.edu) - Louisiana State University; Jiang, Xiuping (xiuping@clemson.edu)- Clemson University; Johnson, Michael (mjohnson@uark.edu) - University of Arkansas; Karunesena, Enusha (enusha.karunesena@ttu.edu)- Texas Tech University; Kniel, Kali (kniel@udel.edu)- University of Deleware; LeJeune, Jeffrey (lejeune.3@osu.edu)- Ohio State University; Marshall, Douglas L. (microman@ra.msstate.edu)- Mississippi State University; Newman, Melissa (mnewman@uky.edu)- University of Kentucky; Price, Stuart B. (pricesb@vetmed.auburn.edu)- Auburn University; Rao, Ramkishan(rrao@CSREES.USDA.GOV)- USDA CSREES; Siebert, Karl (kjs3@cornell.edu)- Cornell University; Subbiah, Jeyamkondan (jsubbiah2@unlnotes.unl.edu) - University of Nebraska; Williams, Rob (rcwillia@vt.edu) - Virginia Tech; Worobo, Randy (rww8@cornell.edu) - Cornell University

I. Self Introductions II. Administrative Advisor Comments - Susan Barefoot Emphasized the importance of completing annual report. Emphasized the importance of completing our updated plan/proposal. Overview of the success of the "Food Safety from the Surface Up" Conference II. USDA CSREES Representative comments - Ram Rao " Suggests Nomination for Secretarys Honors Award " Handout on RFAs  Current " Epi grants are both pre and post harvest " Special emphasis area is retail to consumer in NIFSI- letter of intent for special emphasis grants only III. Group Presentations were given by each of the participants. An overview is listed below and detailed reports are at the end of the minutes. Jim Dickson  Iowa State HACCP validation studies Developed gram negative indicator organisms for O157 Must confirm that they are not pathogenic Antibiotic Resistance in Antibiotic free swine Rob Williams - Virginia Tech UV light and chemical preservatives  apple cider High pressure processing in combination with chemical preservatives for reduction of E. coli and Salmonella in apple juice and orange juice Quantitative recovery of LM and Salmonella from environmental sampling media Prediction of surface area from weight measurement Optimization of recovery and detection for cryptosporidium in foods Dairy and Juice tanker sanitation UV irradiation on reduction of pathogens and chemical indicators of mild Mindy Brashears-Texas Tech University Pre-Harvest Methods Developed Pre-harvest Interventions Post-Harvest Interventions Antibiotic Resistance Studies JM Subbiah  University of Nebraska Modeling temperature changes/loss/cooling. Creating a Web interface Marlene Janes  Louisiana State University Acidified sodium chloride effects on LM in Hams and cheese Copper and brass reductions of LM both inhibited at various temperatures Drain replacement  total counts. 1-3 log reduction on copper surfaces in processing plants Randy Worobo  Cornell Antimicrobial proteins Glocose oxidase system and gluconic acid as a side product Honey microflora  Manuka honey Range of isolates Doug Marshall  Mississippi State Pre and post interventions Aquaculture Growth of Vibrio over season  10 h hold Phage for human therapy Streptococcus iniae  Antibiotic use in aquaculture Stress adaptation of microbiology Salmonella survival in the gut of a shrimp Stewart Price-Auburn BVDV virus and E. coli O157 4 calves  inoculated and mid-week O157 shed at more Jeff Lejune- Ohio State University STEC E. coli Q alleles  human isolates vs bovine isolates Q933 and Q21 isolates less susceptible to processing treatments Toxin negative isolated from humans  still cause illness STEC negative O157  phages can carry toxins to negative and cause them to be pathogenic Use of antibiotics in humans can cause more toxin production. No difference in toxin production after exposure to antibiotics in vitro. Phages that infect O157 are different from O26 and O11 in the US. Melissa Newman- Kentucky Use of decanol as an antimicrobial Use of yeast cell extract as a curing agent for antimicrobial drug resistance Lawrence Goodridge-University of Wyoming Use of bacteriophage in reducing E. coli O157 in cattle HPP in almonds E. coli group present in bovine, but not pathogenic Xiuping Jiang  Clemson (South Carolina) Manure Composting IV. Sections of the Renewal Document were completed to be put together by executive group. Final version will be distributed by January 30, 2006 V. Location of 2006 Meeting Possibilities  Orlando, Nashville, New York See attachment for complete minutes.
[Minutes]

Accomplishments

Objective #1. Pre-harvest reduction of food-borne pathogens in animals and the environment. (Texas Tech) Developed methods to quantify E. coli O157 in cattle feces and hides Validated use of rapid methods for detection of E. coli O157 Mapped the prevalence patterns of E. coli and Salmonella on hides of cattle Determined optimum direct-fed microbial doses for effective inhibition of E. coli O157 in cattle Determined antibiotic resistance patterns in cattle Determined the impact of transportation on cross-contamination Determined the impact of dust on cross-contamination (Auburn) BVDV-infected calves showed anticipated signs of immune suppression, including a marked leucopenia and neutropenia, compared to sham-infected calves. BVDV-infected calves showed higher shedding of O157 compared to sham-infected calves. Most dramatic difference occurred on days 13-15, when BVDV-infected calves were severely leukopenic and neutropenic. (Wyoming) Bacteriophage cocktail can eliminate E. coli O157:H7 in fecal material and may be effective in eliminating E. coli O157:H7 in the live animal. (Louisiana State University) Every summer the oyster industry is threatened by closure of water ways and recall of oysters due to V. vulnificus and V. parahaemolyticus contamination. Our results indicate that bacteriophages active against the virulent Vibrio vulnificus and V. parahaemolyticus are naturally found in oysters and could possibly be used as a processing aid to control these pathogens in live oysters. Bacteriophage treatments alone or in combination with UV-light exposure were effective in reducing V. vulnificus and V. parahaemolyticus population in raw oysters. Objective #2. Chemical and physical decontamination in food processing plant environments. (Texas Tech) Investigated various interventions on beef trim Determined the impact of various interventions on poultry Validated various methods for rapid detection of pathogens in trim, on carcasses and ground beef (Wyoming) Phast Swab is feasible for detection of E. coli O157:H7 on beef carcasses. If a different sampling device is employed, liquid samples can be tested for the presence of E. coli O157:H7 using the Phast Swab. High pressure processing technique is rapid, very effective at reducing Salmonella on the surface of raw almonds, and is amenable to industry use as a method to process raw almonds to reduce microbial load immediately before packaging. (Louisiana State University) Acidified sodium chlorite can be effective as a processing aid for inhibiting the growth of L. monocytogenes counts on ready-to-eat meat products.

Impacts

  1. Approximately $3.7 million in funded projects. List follows:
  2. Impact of Primary Bovine Viral Diarrhea Virus Infection on the Duration and Level of Shedding of Escherichia coli O157:H7. Animal Health and Disease Research Program, College of Veterinary Medicine, Auburn University. PI: Paul H. Walz; Co-investigators: Stuart B. Price, Julie G. Waldrop, James C. Wright, and M. Daniel Givens. 10/1/04 - 9/30/06. $40,000.
  3. Novel Approaches for Reducing Shedding of Salmonella from Chickens. Animal Health and Disease Research Program, College of Veterinary Medicine, Auburn University. PI: Stuart B. Price; Co-investigator, Haroldo Toro. 10/1/05-9/30/07. $36,319.
  4. Pathogen Reduction in Calves Colonized with Salmonella Species. Animal Health and Disease Research Program, College of Veterinary Medicine, Auburn University. PI: James C. Wright. Co-Investigators, Stuart B. Price, Paul H. Walz. 10/1/05-9/30/06. $19,992
  5. Identification and Characterization of Novel Virulence Determinants from Brucella abortus Using In Vivo Induced Antigen Technology (IVIAT). University of Wyoming Agricultural Experiment Station Grants Program. $60,000. January, 2005 to January, 2008. PI. G Andrews, L. D. Goodridge (Co-PI), W. Edwards (Co-PI)
  6. Evaluation of a Bacteriophage Cocktail to Reduce Escherichia coli O157:H7 Shedding in Beef Cattle. National Cattlemens Beef Association, $75,000. February 2005, to May, 2006. PI: L. D. Goodridge, J. Scanga, J. LeJuene
  7. Development of a Luminescent Biosensor for Rapid Detection of Escherichia coli O157:H7.Wyoming National Aeronautics and Space Agency EPSCoR Program, $41,745. March 2005 to March, 2006. L. D. Goodridge
  8. Development of a One-Tube Colorimetric Assay for Detection of Escherichia coli O157:H7 in Food Animals. United States Department of Agriculture National Research Initiative Program. $100,000. September, 2005 to August, 2008. PI: L. D. Goodridge
  9. Development of a Lateral Flow Device for Rapid Chute Side Detection of Brucella abortus infection in Elk. United States Department of Agriculture Special Grants Program. $80,000. September, 2005 to August, 2007. (PI) L. D. Goodridge, G. Andrews (Co-PI), W. Edwards (Co-PI)
  10. Ecology and control of pathogenic strains of Vibrio vulnificus and Vibrio arahaemolyticus in U.S. Gulf Coast oysters. Lee-Ann Jaykus, Andy De Paola, Marlene Janes, Jon Bell, John Supan; Oct. 2004/ Sept. 2007, $1.4 million (LSU Ag Center portion $415,504), Funded.
  11. Control of Listeria monocytogenes in crawfish and shrimp processing facilities using copper, copper-based alloys or coatings containing copper ions. PI as M. E. Janes, Co- PI J. Bell, USDA Aquaculture Special Grants Program; 09/05 to 09/06, $24,000.
  12. Detection of Vibrio vulnificus by direct colony immunoblot PI as M. E. Janes, Co-PI J. Simonson and J. Bell. National Louisiana Sea Grant College Program 06/05 to 07/07, $94,537
  13. Increasing the survival and shelf-life of Gulf Coast Oyster by controlling air exposure: Potential role of stress proteins. Gulf Oyster Industry PI as J La Peyer and Co-PI M. E. Janes National Louisiana Sea Grant College Program 06/05 to 07/07 $192,126
  14. Efficacy of Post-Processing Interventions for the Reduction of Listeria monocytogenes on Frankfurters. Robert C. Williams, David A. Golden (University of Tennessee), Susan S. Sumner, Svetlana Zivanovic (UT), P. Michael Davidson (UT). USDA- IREE-CGP-NIFSI. $599,895
  15. Norwalk Virus Inactivation by High Hydrostatic Pressure Processing: A Comprehensive and Integrated Program for Research and Outreach. George Flick, Daniel Holliman, Angela Correa. USDA- IREE-CGP-NIFSI. $600,000.
  16. Food Safety from the Surface Up: A Conference. PI: Susan F. Barefoot, Co-I: P.L. Dawson, E.H. Hoyle. USDA CSREES NIFSI. October 2004- September 2006. $31,500

Publications

See minutes attachment
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