Brief Summary of Minutes of Annual Meeting

Accomplishments

 Objective 1: Identify Mechanisms that Regulate Ovarian Function and Oocyte Quality during the Estrous Cycle

• Explored the impact of altered insulin and glucose observed in dairy cattle experiencing heat stress (impact on the reproductive tract and elsewhere throughout the body). 
• Investigated the contribution of the preovulatory follicular environment to oocyte quality and competency for embryonic development.  Studies have described the relative contributions of steroid hormones as well as undefined components of the follicular fluid.
• Evaluated the effect of FSH treatment duration during MOET regimen in cattle with low AFC phenotype.
• Explored mechanisms responsible for the improvement in fertility that is observed following displayed estrus in a fixed-time artificial insemination protocol.  This work determined that the follicular fluid contributes to fertility under these circumstances as blastocyst development was greater when oocytes were exposed to follicular fluid from animals that did display estrus (compared to those that did not).
• Determined the effects of thrombin and calcium ionophore, alone and in combination, on CCN1 expression, in steroidogenic cells obtained from the midcycle bovine corpus luteum.
• Determined the effects of thrombin and calcium ionophore, alone and in combination, on VEGF expression, in steroidogenic cells obtained from the midcycle bovine corpus luteum.
• Determined the effects of the phytoestrogen, equol, on progesterone (P4) production in bovine corpora lutea.
• Explored metabolomic signatures of ovarian development in cattle
• Determining the role of melatonin on oocyte development in-vivo and the potential for melatonin to improve quality of oocytes in-vitro
• Generated preliminary evidence that melatonin is present in follicular fluid and plans to publish this novel finding. The next study will examine follicular fluid melatonin concentrations and oocyte quality in lactating dairy cattle.
• Described orphan nuclear receptor SF-1 as a potential novel regulator of ovarian reserve establishment in cattle.
• Examined the modulation of apoptotic mechanisms in developing bovine corpora lutea as regulating susceptibility to luteolysis in response to PGF2A.
• Described the role of potassium ions in oocyte maturation.
• Evaluated the dose-dependent effects of follicle-stimulating hormone (FSH) on ovarian response and in vitro embryo production (IVP) in prepubertal Holstein heifers.
• We have determined how chemical exposures negatively impacts ovarian function in pre- and post-pubertal female pigs and have identified targets of heat stress in the ovary that are altered to impair fertility.
• Identification of factors associated with higher estrous associated temperatures in beef cattle.
• Identification of advanced follicular progression and differential metabolite profiles in beef heifers with varied levels of higher estrous associated temperatures and sexually active behavior.
• Identification of differential transcript profiles in bovine embryos matured in the presence of greater vs lesser maturity that indicate superior metabolism, oxidative balance, and WNT signaling in embryos resulting from oocytes matured in the presence of greater maturity follicular fluid that may explain some of the difference in embryo quality and pregnancy establishment between follicles of greater and lesser maturity.
• Identification of differential metabolome profiles in preovulatory follicles of greater vs lesser maturity at the time of GnRH administration that may contribute to superior pregnancy outcome in cows induced to ovulate a follicle of greater maturity.
• Examined combinatorial exposures (i.e. chemicals with heat stress) to determine additive impacts.
• Identified novel ovarian causes of chemical dysfunction. The impacts of this research are making discoveries that benefit scientific, clinical and production animal communities.
• We have disseminated our findings through manuscript publication, and through presentations at national and international conferences. 
• Benefit to the broader public is that improved reproductive function in female animals is needed for a healthy and sustainable protein food source supply and animal welfare is improved by enhanced female reproductive health.
• Determined effects of luteinizing hormone/protein kinase (PKA) signaling on metabolic pathways driving function of luteal cells and progesterone production.
• Determined signaling and metabolic pathways regulating the function of granulosa cells. We determined the PKA-induced metabolic changes and content of related proteins as well as function of glucose metabolism branches on granulosa cell function.
• Determined the role of metabolites as signaling molecules in the somatic ovarian cells. We found that main kinase triggered by gonadotropins, such as protein kinase A (PKA), enhances histone acetylation and increases chromatin availability in the granulosa cells. 
• Explored the regulation of chemokine expression from luteal cells after prostaglandin F_2a.

  Objective 2: Determine Factors Associated with Fertilization, Embryo Development, and Conceptus-Endometrial Interactions that Dictate Pregnancy Success

• Explored how various members of the interleukin-6 (IL6) cytokine family influences development of the embryonic disk to begin to decipher the mechanisms at play that produce beneficial effects of IL6 supplementation on early conceptus development.
• Used gene editing technologies to show that embryo-derived IL6 family members, and likely IL6 itself, is required for normal embryogenesis in cattle.
• Developed a bovine model to increase the number of small antral follicles during the recruitment phase of a follicular wave in cattle with low AFC phenotype.
• The use of antioxidants to improve in vitro bovine embryo production was explored.
• Evaluating melatonin concentrations in follicular fluid and blood circulation with pregnancy success and early pregnancy loss in lactating dairy cattle.
• Investigated the role of uterine perfusion in corpus luteum maintenance during pregnancy in cattle.
• Characterizing adenomyosis (ectopic endometrial glands and stroma in the myometrium) in postpartum dairy cows and is investigating its effect on fertility. Our studies have found an association between postpartum metritis and the severity of adenomyosis, including increased fibrosis, which may impact the receptivity of the uterus to an embryo.
• Investigating the impact of metritis/endometritis on postpartum endometrial epithelial regeneration and the effects on fertility. Our studies have identified a putative endometrial epithelial stem/progenitor cell population that is expanded in diseased endometrium in an attempt to repair the luminal epithelium which is required for pregnancy establishment.
• Developing an animal model that will investigate the effects of preovulatory estradiol concentrations in the DNA methylation profile and cellular subpopulations in the uterus. So far, we have validated a protocol to harvest luminal epithelial cells and submit them to single cell RNAseq. The next step is to validate the animal model with high and low estradiol concentrations, and after that collect the uterine samples.
• Determined that elevated progesterone (P4) for a limited time (D14-19) increases fertility in heifers that are recipients of IVF embryos.
• Associated fertility traits with markers of oocyte competence in dairy cattle.
• Investigating the role of methyl donors during the periconceptional period and their influence on embryonic development, pregnancy establishment, offspring development, and epigenetic modifications such as DNA methylation.
• Exploring the mechanisms governing bovine conceptus elongation. Our overarching long-term goal is to recapitulate this phenomenon in vitro.
• Expanded on previous studies demonstrating vitamin A and D injectable supplementation increases success at first breeding post-parturition in first and second calf Angus and Angus cross-bred heifers to evaluate effects of vitamin E alone or in combination with A and D on breeding success following first cycle artificial insemination.
• Identification of endometrial biological responses specific to IVP cattle embryos and IVP cattle embryo specific molecules that elicit these responses that may explain why IVP cattle embryos suffer from a high rate of embryonic loss after transfer.
• Establishment of CRISPR-Cas9 technology to genetically edit bovine embryos and test the function of two embryo proteins (galectin-1 and -3) thought to be necessary for pregnancy and that could be utilized as a technology to improve pregnancy success.
• Investigating the application of Sperm Energy-restriction and Recovery (SER) prior to in vitro fertilization (IVF) as a strategy to improve bovine embryo production. Preliminary results indicate that SER treatment enhances sperm motility without affecting fertilization rates and improves embryo development to the blastocyst stage.
• Ongoing studies are evaluating SER as a modulator of the sperm-specific phospholipase C zeta 1 (PLCζ1), the primary sperm-derived oocyte activation factor, as well as the potential contribution of secondary sperm factors such as phospholipase C delta 4 (PLCδ4) to successful fertilization in cattle.
• Developing and implementing novel approaches to assess sperm function and to investigate how sperm quality and physiology influence post-fertilization events and early embryonic development.

 Objective 3: Develop and Evaluate Novel Reproductive Management Strategies and Technologies to Improve Reproductive Performance of Ruminants

• Determined the effect of a targeted reproductive management programs based on automated detection of estrus during the voluntary waiting period on reproductive performance of lactating dairy cows
• Provided evidence that combining reproductive outcomes predictors and automated estrus alerts recorded during the voluntary waiting period identified subgroups of cows with different reproductive performance potential.
• Evaluated a rapid resynchronization program allowing timed embryo transfer every 21d.
• Explored the effect of timing of AI with conventional or sex-sorted semen on fertility of dairy cows.
• Demonstrated that delayed induction of ovulation affects the ovarian function of lactating dairy cows synchronized with the Double-Ovsynch protocol and increases insemination after detected estrus.
• Developed novel reproductive management programs aimed at increasing fertility of first service through promotion of expression of estrus at the end of synchronization of ovulation protocols.
• Exploring the use of circulating melatonin concentrations before artificial insemination as a predictor of pregnancy success in lactating dairy cattle. 
• Identified associations between milk production potential and the profitability of primiparous Holstein cows submitted to insemination with programs that prioritized insemination at detected estrus or timed artificial insemination
• Refined lateral flow-based electronic system for determination of the pregnancy and ovarian status of cattle.
• Explored ways to optimize synchronization protocols to maximize fertility and reproductive efficiency.
• In collaboration with ST Genetics, submitted a provisional patent application entitled “Amniotic fluid metabolic biomarkers and methods of use”.
• Identification of sperm metabolites that differ in abundance between Angus bulls of high and low field fertility that may be used as biomarkers of ejaculate quality.
• Continued efforts for the development and evaluation of innovative reproductive technologies aimed at improving fertilization outcomes and embryo production efficiency in cattle.
• Validating accelerometers devices for use in beef cattle. The next step is to define what is the best time to perform artificial insemination in beef cattle when accelerometer devices are used for estrus detection.
• Performed one experiment testing how many times intravaginal progesterone devices can be reused in beef cattle submitted to a 7-day synchronization protocol.
• Studied the use of anogenital distance (distance from the center of the anus to the clitoris) as a marker of puberty attainment and probability of pregnancy by artificial insemination in beef heifers.
• Working towards optimization of whole ovary cryopreservation in ewes.
• Understand the relationship between feed efficiency and seminal quality in beef bulls.
• Evaluated a novel recombinant hormone protocol for embryo production in Holstein heifers using a single injection of long-acting human follicle-stimulating hormone (FSH).
• Assessing the use of low-dose human chorionic gonadotropin (hCG) at the time of progesterone device withdrawal to support follicular development.

In addition to Hatch Multistate Funds, these studies were supported by:

• Biotracking LLC
• FFAR Seeding Solutions Grant: 24-001130
• Illinois Beef Checkoff
• Merck Animal Health
• NAAB Doak Graduate Fellowship
• NIH-NICHD
• NIH-NIEHS
• NIH/NIGMS-NRSA-T32
• NSF
• Texas A&M AgriLife Research
• University of Missouri CAFNR Equipment Grant
• University of Tennessee AgResearch Instrumentation Grants Program
• University of Tennessee Genomics Center for the Advancement of Agriculture Seed Grant
• USDA-NIFA-AFRI
• USDA-NIFA-OREI
• Virginia Agricultural Council Research Funding
• Virginia Tech John L. Pratt Animal Nutrition Program
• WTAMU Killgore Research Grant
• Zoetis

Peer-reviewed journal articles (2025)

• Absalon-Medina VA, Sala RV, Pereira DC, Fricke VC, Devkota I, Bonomo ZL, Fuego DM, McDonald MM, Sánchez JM, Rabaglino MB, Matsakas A, Vourekas A, Fu X, Rivera RM, Lonergan P, Ross PJ and Simintiras CA. Amniotic fluid metabolic biomarkers of fetal physiology and pregnancy success. Biology of Reproduction. doi: 10.1093/biolre/ioaf236.
• Alward KJ, Duncan AJ, Ealy AD, Dahl GE, Petersson-Wolfe CS, Cockrum RR. Changes in photoperiod during the dry period impact colostrum production in Holstein and Jersey cows. J Dairy Sci. 2025 Feb;108(2):1672-1685. doi: 10.3168/jds.2024-25415.
• Alward, KJ, Graves, W M., Palomares, RA and JF Bohlen. 2025. Evaluating the relationships between anti-Müllerian hormone, milk production, estrous expression and first service conception rate in lactating dairy cows. Therio. doi: 10.1016/j.theriogenology.2025.117598.
• Andrade JPN, Domingues RR, Gomez-León VE, Madureira G, Sartori LC, Grillo GF, Fosado M, Sala RV, Wiltbank MC. Delayed embryonic attachment, circulating pregnancy-associated glycoproteins, and pregnancy loss in heifer embryo recipients. Theriogenology 2025; 247. ARTN 117561 10.1016/j.theriogenology.2025.117561
• Andrade JPN, Gomez-León VE, Madureira G, Sartori LC, Grillo GF, Domingues RR, Fosado M, Sala R, Wiltbank MC. ReBreed21-ET: Evaluation of a rapid resynchronization program that allows timed embryo transfer every 21 days. Theriogenology 2025; 235:145-151. 10.1016/j.theriogenology.2024.12.031
• Bennett, A., Rocha, CC., Waheed, A., Mesquita, FS., Maia, TS., Haimon, ML.-J., Hoorn, QA., Sagheer, M., Cuellar, CJ., Sultana, H., Ojeda-Rojas, OA., Krisher, RL., Rubessa, M., Pohler, KG., Hansen, PJ., Moriel, P., Chebel, RC., and Binelli, M (2025). Assessment and Prediction of Embryo Survival in Fertility-Classified Beef Cows. Theriogenology, 117560. doi: 10.1016/j.theriogenology.2025.117560
• Binelli M, Lopez-Duarte MC, Gonella-Diaza A, Silva FACC, Pugliesi G, Martins T, Rocha CC. Effectors and predictors of conceptus survival in cattle: What is next? Domest Anim Endocrinol. 2025 Jul;92:106939. doi: 10.1016/j.domaniend.2025.106939. PMID: 40064052.
• Buol, BM, Antwi-Boasiako, C, Mayorga, EJ, Gonzalez-Alvarez, MS, Baumgard, LH, Keating, AF 2025. Effects of perfluorooctanoic acid exposure and heat stress on performance and liver health biomarkers in post-pubertal gilts. Journal of Animal Science. doi: 10.1093/jas/skaf348.
• Chasi B, Andrade JP, Monteiro PLJ, Castro B, Moreno E, Anta E, Ali NN, Cole JB, Wiltbank MC, Balboula AZ, Ortega MS. Association of fertility traits with markers of oocyte competence in dairy cattle. Journal of Dairy Science 2025; 108:12814-12823. 10.3168/jds.2025-26726
• Consentini CEC, Abadia T, Galindez JPA, Lopes ALM, Ferro PPC, Pazini YE, Faria N, Machado F, Capella T, dos Santos TN, Duarte M, Ferreira PP, Matos LMF, Ferreira DR, Campos E, Prata A, Melo LF, Wiltbank MC, Sartori R Fertility programs for lactating dairy cows: A novel presynch plus timed artificial insemination program (Double E-Synch) produces similar ovarian dynamics, synchronization, and fertility as Double-Ovsynch. Journal of Dairy Science 2025; 108:4435-4447. 10.3168/jds.2024-25221
• de Castro Lourenço V, de Oliveira L, Maldonado MBC, Rolniche LCM, de Oliveira Bezerra L, Feltrin IR, Morelli KG, Mendes AF, Rocha CC, Castilho C, Bridi A, Pugliesi G, Ealy AD, Nogueira MFG, Membrive CMB. Oleic acid supplementation increases the PGE2:PGF2α ratio and alters expression of prostaglandin biosynthesis and maternal pregnancy recognition genes in bovine trophoblast cells in vitro. Theriogenology. 2025 Oct 1;245:117503. doi: 10.1016/j.theriogenology.2025.117503.
• Gabel A and Diaz FJ. Zinc deficiency in bovine mural granulosa cells results in hippo pathway-mediated apoptosis, Biology of Reproduction, 2025. ioaf222, doi: 10.1093/biolre/ioaf222
• Garcia-Guerra A, Motta J C L , Sala R V., Hayden C B , Ponte E., Absalon-Medina V A., Ross P J 2025. Optimizing In Vitro Embryo Production in Cattle: Strategies for Donor Management and Selection. Animal Reproduction, 22(3):e20250079. doi: 10.1590/1984-3143-AR2025-0079.
• Giordano J O 2025. Data-driven technologies and management practices for improving the sustainability of reproductive management. Reproduction, Fertility and Development. Reproduction, Fertility and Development 37, RD24148 doi: 10.1071/RD24148
• González-Brusi L, Devkota I, Sánchez JM, Lamas-Toranzo I, Lonergan P, Bermejo-Álvarez P and Simintiras CA. Bovine uterine fluid lipidome parallels embryo and conceptus developmental milestones. Biology of Reproduction. doi: 10.1093/biolre/ioaf194.
• Good, SL., Antwi-Boasiako, C., González-Alvarez, ME., Buol, BM., Baumgard, LH., Keating, AF., Charbonnet, JA 2025. Distribution of perfluorooctanoic acid in exposed female post-pubertal pigs in thermal neutral or heat stressed conditions. Toxicological Sciences. 205(1):143-153. doi: 10.1093/toxsci/kfaf013.
• Harl AW, Negrón-Pérez VM, Stewart JW, Perry GA, Ealy AD, Rhoads ML. Follicular fluid from cows that express estrus during a fixed-time artificial insemination protocol promotes blastocyst development. J Dev Biol. 2025 Apr 25;13(2):14. doi: 10.3390/jdb13020014.
• Harl AW, Negrón-Pérez VM, Stewart JW, Perry GA, Ealy AD, Rhoads ML. Maturation of bovine cumulus oocyte complexes in follicular fluid with or without estradiol, progesterone or the combination affects cumulus cell expansion and blastocyst development. PLoS One. 2025 Jun 2;20(6):e0321266. doi: 10.1371/journal.pone.0321266.
• Hayden C B, Motta J C L , Sala R V., Bello N., Coutinho da Silva M., García-Guerra A 2025. Efficacy of methods to synchronize follicular wave emergence in pregnant heifers. JDS Communications, 6(1): 154-159. doi: 10.3168/jdsc.2024-0629.
• Heredia D, Tarnonsky F, Venturini M, Lopez-Duarte MC, Fernandez-Marenchino I, García-Guerra A, Luchini D, Hansen PJ, Pringle D, Dilorenzo N, Gonella-Diaza AM. Impact of dietary supplementation of beef cows with rumen-protected methionine during the periconceptional period on post-weaning performance of female offspring. J Anim Sci. 2025 Jan 4;103:skaf380. doi: 10.1093/jas/skaf380. PMID: 41165602.
• Hester JM, and Diaz FJ. DTPA Disrupts Development of Preantral Ovarian Follicles in vitro. Reproduction and Fertility 6.4 (2025), e240125. doi: 10.1530/RAF-24-0125.
• Hoorn QA, Kenny DA, Fair S, Lonergan P and Simintiras CA. Metabolic profiling identifies fertility markers in bull sperm. Biology of Reproduction. doi: 10.1093/biolre/ioaf234.
• Hughes CHK, Hellmers AC, daSilva MI, Ott TL, Pate JL. IFNT-dependent and -independent changes in the CL of early pregnancy. Biol Reprod 2025; ioaf044. doi: 10.1093/biolre/ioaf044.
• Izquierdo VS, Vendramini JMB, Ashrafi N, Mimi RA, Graham SF, Gonella-Diaza A, Moriel P Heat stress mitigation during prepartum and postpartum altered the plasma metabolome in Bos indicus-influenced cow-calf pairs. J Anim Sci. 2025 Jan 4;103:skaf195. doi: 10.1093/jas/skaf195. PMID: 40506825;
• Keane JA, Wooldridge LK, Oliver MA, Speckhart SL, Alward KJ, Nix JL, Biase FH, Ealy AD. Modifications in media composition and antioxidant support influences in vitro produced bovine blastocyst inner cell mass and trophectoderm numbers. Theriogenology. 2025 Oct 15;246:117532. doi: 10.1016/j.theriogenology.2025.117532.
• Klabnik JL, Beever JE, Payton RR, Lamour KH, Schrick FN, Edwards JL. A step toward understanding direct impacts of a higher estrus associated temperature (HEAT): Transcript level changes in cumulus-oocyte complexes directly exposed to acute elevated temperature. Animals. 2025. 15(4), 517 doi: 10.3390/ani15040517.
• Laplacette, A L., Stangaferro, M L., Thomas, M J., and Giordano, J O. 2025. Effect on first service outcomes of a modified Double-Ovsynch protocol designed to increase fertility through insemination of cows in estrus and a targeted intervention for non-estrus cows. J Dairy Sci. (In press). https://doi: 10.3168/jds.2025-27396
• Laplacette A L., Rial C., Sitko E M., Perez M M., Tompkins S., Stangaferro M L., Thomas M J., and Giordano J O. 2025. Delaying induction of ovulation and timed artificial insemination in a Double-Ovsynch protocol increased expression of estrus and altered first-service reproductive outcomes of lactating dairy cows. J Dairy Sci. 108(1), 1103–1124. https://doi: 10.3168/jds.2024-24994
• Leavitt ML, Patterson AL, Wagle MD, Warren WC, Spencer TE, Davenport KM. Cross-species integration of single-cell transcriptomics reveals conserved mechanisms driving post-implantation placental development in ruminants. Scientific Reports. 2025 Nov 26;15(1):45435. doi: 10.1038/s41598-025-29895-2.
• Luo WX, Wiltbank-Chau LB, Shrestha HK, Wiltbank MC. Differential responsiveness and signaling pathways for prostaglandin F2a on chemokine mRNA in bovine corpus luteum and luteinized granulosa cells. Domestic Animal Endocrinology 2025; 93. ARTN 106962 10.1016/j.domaniend.2025.106962
• Maldonado, MBC., Cabrini, DC., Bezerra, LO., Rolniche, LCM., Lourenço, VC., Feltrin, IR., Morelli, KG., Mendes, AF., Rocha, CC., Bridi, A., Pugliesi, A., Nogueira, MFG., Membrive, CMB (2025). Conjugated linoleic acid supplementation: Insights into prostaglandin synthesis and vitro embryonic development. Animal Reproduction Science, 107990. doi: 10.1016/j.anireprosci.2025.107990.
• McDonald KS, Drum JN, Moraes JGN, Melton C, Ojeda-Rojas OA, Venturini M, Gonella-Diaza AM, Prather RS, Ortega MS. Effect of FGF2, LIF, IGF1 supplementation on pregnancy success following embryo transfer of in vitro derived embryos. Theriogenology. 2025 Oct 1;245:117533. doi: 10.1016/j.theriogenology.2025.117533. PMID: 40513373.
• Molins Colomer M, Hughes CHK, Casella E Non-Invasive Thermal Monitoring of Dairy Cows' Core Body Temperature with Multi-Modal Sensing. IEEE MetroAgriFor 2025. doi: 10.1109/MetroAgriFor66923.2025.11512516.
• Morin F, Hughes CHK, Roussel V, Gevry N, Murphy BD. Double knockout of steroidogenic factor 1 (SF-1; NR5A1) and liver receptor homolog 1 (LRH-1; NR5A2) in the mouse ovary results in infertility due to disruption of follicle development and ovulation. Biol Reprod 2025; 113: 182-198. doi: 10.1093/biolre/ioaf079
• Motta JCL., Sala RV., Hayden CB., Pereira DC., Fricke VC.; Absalon-Medina VA., Ross PJ., García-Guerra A Ovarian superstimulation for in vitro embryo production based on Anti-Müllerian hormone phenotype: Effect of FSH dose schedule. Animal Reproduction Science. 2025. 281: 107980. doi: 10.1016/j.anireprosci.2025.107980
• Motta JCL., Hayden C B., Sala R V., Ross P J., Garcia-Guerra A 2024 Advances in synchronization and superstimulation for OPU/IVEP: Optimizing oocyte quantity and quality. Reproduction, Fertility and Development, 37: RD24143. doi: 10.1071/RD24143.
• Mörig, F., Drillich, M., Burnett, T A., Bruinjé, T C., Giordano, J O., Madureira, A M L., and Borchardt, S 2025. Evaluation of the association between automated estrus alerts from activity monitoring systems in early lactation with reproductive performance in lactating Holstein cows: A meta-analysis. J Dairy Sci. (In press). https://www.journalofdairyscience.org/article/S0022-0302(25)00966-X/fulltext
• Negrón-Pérez VM, Al Naib A, Zezeski AL, McCracken-Harlow VL, Perry GA, Ealy AD, Rhoads ML. Cumulus cell expansion, nuclear maturation and embryonic development of bovine cumulus-oocyte complexes matured in varying concentrations of follicular fluid. PLoS One. 2025 Feb 7;20(2):e0318376. doi: 10.1371/journal.pone.0318376.
• Newman, ML., Primo, VAB., Ugarte Marin, MB., Peixoto, PMG., Gonzalez, TD., Bennett, A., Rocha, CC Binelli, M., Bishop, JV., Guzeloglu, A., Hansen, TR., Thatcher, WW., Bisinotto, RS (2025) Interferon-tau concentration at the vaginal fornix and cervical ostium predicted early pregnancy outcomes in lactating dairy cows, Biology of Reproduction, ioaf251, doi: 10.1093/biolre/ioaf251
• Oliver MA, Alward KJ, Rhoads ML, Ealy AD. Human Recombinant Interleukin-6 and Leukemia Inhibitory Factor Improve Inner Cell Mass Cell Number but Lack Cryoprotective Activities on In Vitro-Produced Bovine Blastocysts. Animals (Basel). 2025 Feb 25;15(5):668. doi: 10.3390/ani15050668.
• Peterson KD, Oliver MA, Freeman TF, Poudel SP, Behura SK, Kakhniashvili D, Johnson DL, Payton RR, Prado TM, Strickland LG, Edwards JL, Shepherd EA, Beever JE, Spencer TE, Mathew DJ. Bovine endometrium drives and responds to divergence of in vitro produced conceptus biochemistry. The FASEB Journal. 2025. doi: 10.1096/fj.202501962R.
• Peterson KD, Freeman TF, Poudel SP, Behura SK, Kakhniashvili D, Johnson DL, Prado TM, Strickland LG, Beever JE, Spencer TE, Mathew DJ. 2025. Unraveling the elongating bovine conceptus microenvironment: Identification of gene transcripts and proteins along the conceptus-maternal interface in cattle. Biology of Reproduction. 2025. doi: 10.1093/biolre/ioaf002. Featured in the
• Przygrodzka E., Bhinderwala F., Powers R., McFee RM., Cupp AS., Wood JR., Davis JS Metabolic control of Luteinizing Hormone-responsive ovarian steroidogenesis. J Biol Chem. J Biol Chem. 2025 Jan;301(1):108042.
• Rishi, JK., Montes, C., Walley, JW., Keating, AF 2025. Ovarian SUMO-2/3 targets and their differential response to genotoxic stress induced by 7,12-dimethylbenz(a)anthracene exposure in lean and obese female mice. Biology of Reproduction. 113(4):962-976. doi: 10.1093/biolre/ioaf101.
• Rocha, CC., Silva, FACC., Cavani, L., Cordeiro, ALL., Maldonado, MBC., Bennett, A., Waheed, A., Campbell, M., Pohler, KG., Peñagaricano, F and Binelli, M (2025), Endometrial Gene Expression Predicts Pregnancy Outcome in Brahman Cows. Molecular Reproduction and Development, 92: e70047. doi: 10.1002/mrd.70047
• Rocha, CC., Montevecchio, AB., Bennett, A., Waheed, A., Mazziotta, M., Maia, TS., Haimon, MLJ., Hoorn, QA., Sagheer, M., Cuellar, CJ., Ojeda-Rojas, OA., Krisher, RL., Rubessa, M., Pohler, KG., Hansen, PJ., Moriel, P., Chebel, RC., and Binelli, M (2025). Relationships between activity monitoring device data and ovarian, uterine, hormonal, and pregnancy variables in beef cows. (2-25). Theriogenology. doi: 10.1016/j.theriogenology.2025.01.001
• Sagheer M, Haimon MLJ, Montoya SH, Heredia D, Tarnonsky F, Venturini ME, Gonella-Diaza A, DiLorenzo N, McFadden JW, Dalmaso de Melo G, Pohler KG, Hansen PJ. Feeding rumen-protected choline during the periconceptional period programs postnatal phenotype of suckled beef calves. J Anim Sci Biotechnol. 2025 Apr 2;16(1):48. doi: 10.1186/s40104-025-01188-8. PMID: 40170061.
• Santos VG, Carvalho PD, Souza AH, Priskas S, Castro JAL, Pereira AMF, Ross PJ, Moreno J, Wiltbank MC, Fricke PM. Effect of timing of artificial insemination with conventional or sex-sorted semen on fertility of lactating dairy cows. Journal of Dairy Science 2025; 108:8945-8958. 10.3168/jds.2025-26428.
• Schultz, RB., Adur, MA., Kiefer, ZE., Studer, JM., Bidne, KL, Romoser, MR., Baumgard, LH., Keating, AF., Ross, JW 2025. The effect of pregnancy, heat stress and altrenogest supplementation on the porcine mid-luteal phase corpus luteum proteome. Theriogenology. 246:117536. doi: 10.1016/j.theriogenology.2025.117536.
• Sellmer Ramos I, Caldeira MO, Poock SE, Moraes JGN, Lucy MC, Patterson AL. Adenomyosis and fibrosis define the morphological memory of the postpartum uterus of dairy cows previously exposed to metritis. Journal of Dairy Science Communications. 2025 Mar;6(2):250-255. doi: 10.3168/jdsc.2024-0633.
• Shawky AEM, Scarboro A, Avanzino K, Simintiras CA, Hatzoglou M and Vourekas A. DDX3X as a selective dual switch regulator of mRNA translation in early response to stress. Cell Reports. doi: 10.1016/j.celrep.2025.116493.
• Silva GM, Podversich F, Silva AEM, Macias Franco A, Gonella-Diaza A, Mateescu RG, DiLorenzo N Short communication: thermotolerance and residual feed intake in Bos-indicus crossbred beef heifers. Transl Anim Sci. 2025 Apr 15;9:txaf051. doi: 10.1093/tas/txaf051. PMID: 40463911;
• Soto-Moreno EJ, Ali NN, Küllmer F, Nasufovic V, Frolikova M, Tepla O, Masata J, Trauner D, Patterson AL, Arndt HD, Komrskova K, Zernicka-Goetz M, Glover DM, Balboula AZ. Spindle-localized F-actin regulates polar MTOC organization and the fidelity of meiotic spindle formation. Nature Communications. 2025 Sep 19;16(1):8323. doi: 10.1038/s41467-025-63586-w.
• Speckhart SL, Pollock AB, Alward KJ, Farrell K, Oliver MA, Lee K, Biase FH, Ealy AD. The interleukin-6 signal transducer receptor subunit is required for optimal in vitro bovine embryo development. Biol Reprod. 2025 Mar 16;112(3):434-446. doi: 10.1093/biolre/ioaf006.
• Stokes, AE, Clark HM, Edwards JL, Payton RR, Beever JE, Freeman TF, Schrick FN, Hessock EA, Moorey SE. Transcriptome profiles of blastocysts originating from oocytes matured in follicular fluid from preovulatory follicles of greater or lesser maturity. BMC Genomics. 2025. 26: 339. doi: 10.1186/s12864-025-11521-0.
• Timme, K., Inyang, I., White, HE., Keating, AF 2025. Diet-induced obesity alters the ovarian chemical biotransformation and oxidative stress response proteins both basally and in response to 7,12-dimethylbenz[a]anthracene. Toxicological Science. 204(1):9-19. doi: 10.1093/toxsci/kfae150.
• Venturini M, de Agostini Losano J, Heredia D, López Duarte MC, Infante J, Podversich F, McGraw M, Siddique MS, Guertin J, Poliakiwski B, Smith DJ, Pohler K, DiLorenzo N, Daigneault BW, Gonella Diaza A Residual feed intake and reproductive-related parameters in yearling Brangus bulls. J Anim Sci. 2025 Jan 4;103:skaf055. doi: 10.1093/jas/skaf055. PMID: 39989134;
• Walsh AA, Morozyuk M, Schettini GP, Mercadante VRG, Ealy AD, Biase FH. Frozen media preserves blastocyst development and quality in cattle embryos produced in vitro: a six-month evaluation. Theriogenology. 2025 Nov;247:117595. doi: 10.1016/j.theriogenology.2025.117595.

Book Chapters (2025)

• Giordano O. Reproductive strategies for dairy cattle in Encyclopedia of Livestock Medicine for Large Animal and Poultry Production. 2025. First Edition. Editor: João Simões. Publisher: Springer Cham. ISBN 978-3-031-52133-1 https://doi.org/10.1007/978-3-031-52133-1

Conference Proceedings (2025)

• Giordano J. O. Enhancing reproductive performance and management of cows with different reproductive potential through data-driven technology. Israeli Cattle Breeders Association Annual Conference. Jerusalem, Israel. December 2^nd, 2025.
• Giordano J. O. Challenges and opportunities in reinsemination programs: combining biology and management to improve reproductive success. ANEMBE (National Association of Spanish Bovine Practitioners). May 29^th, 2025. Vittoria, Spain.
• Giordano J. O. Genomics and reproduction: biology, management, and profitability of cows with different genetic potential for fertility. ANEMBE (National Association of Spanish Bovine Practitioners). May 29^th, 2025. Vittoria, Spain.
• Giordano J. O. Improving dairy herd health monitoring and management using automated monitoring technologies. Western Canadian Dairy Seminar. Red Deer, Alberta, Canada. March 4^th, 2025.
• Giordano J. O. Monitoring reproductive programs using herd records. Delivered in English and Spanish. Dairy Manager School, Alta Genetics. Garden City, Kansas. November 18^th, 2025.
• Giordano J. O. Reproductive management of lactating dairy cows. Delivered in English and Spanish. Dairy Manager School, Alta Genetics. Garden City, Kansas. November 18^th, 2025.
• Giordano J. O. Automated monitoring technology for dairy cows. Automated Dairy Herd Health Monitoring Outreach Program for NY Dairy Farms. Sponsored by New York Farm Viability Institute. Harford, New York. October 24^th, 2025.
• Giordano J. O. The intersection of biology and data-driven technology for (cow) precision reproductive management. CoRe - Cornell Reproductive Biology Seminar. Ithaca, New York. October 28^th, 2025.
• Giordano J. O. Targeted reproductive management of dairy cattle. Danone Milk Academy. Celine, Ohio. October 28^th, 2025.
• Giordano J. O. Targeted reproductive management. Merck Workshop for Technical Services Team. Kansas City, Missouri. September 23^rd, 2025.
• Giordano J. O. Dairy tech research at Cornell University. Precision Dairy Management Conference. Bloomington, Minnesota. June 18^th, 2025.
• Giordano J. O. and M. M. Perez. The present and future of technology for automated herd monitoring and management at CAST for the Farm of the Future. Western New York Dairy Day. Perry, NY. February 4^th, 2025.
• Giordano J. O. Data-driven technologies and management practices for improving the sustainability of reproductive management. International embryo technology society. Forth Worth, TX. January 21^st
• Giordano J. O., C. Rial, A. L. Laplacette, M. M. Perez, and E. M. Sitko. Enhancing reproductive performance and management of cows with different reproductive potential through data-driven technology. Ohio Dairy Veterinarians annual meeting. Columbus, OH. January 10^th
• Patterson AL. Unraveling Cellular Plasticity in Endometrial Regeneration. The Society for Reproductive Investigation 72^nd Annual Meeting, Charlotte, NC, March 25-29, 2025.
• Rocha, C.C. Branco, L.CS. Binelli, M. Using automated monitoring devices for estrus detection in embryo transfer programs. (2025) American Embryo Transfer Association, October 16-18, Reno- Nevada, USA.

Abstracts (2025)

• Adeyanju O, González-Alvarez ME, Antwi-Boasiako C, Keating AF. 2025. Obesity amplifies the reproductive toxicity of perfluorooctanoic acid (PFOA): disruptions in non-gravid uterine protein abundance and apoptotic pathways. Society of Toxicology Annual Meeting.
• Alward KJ, Pollock AB, Ealy AD. 2025. Antral follicle count and dominant follicle size in dairy cattle. American Dairy Science Association Annual Meeting, June 22–25, 2025; Louisville, KY, USA.
• Antwi-Boasiako C, González-Alvarez ME, Buol BM, Adeyanju OZ, Baumgard LH, Keating AF. 2025. Investigating ovarian effects of perfluorooctanoic acid in post-pubertal pigs during thermal neutral and heat stress conditions. Society of Toxicology Annual Meeting.
• Arroyo-Salvo CA, Río S, Boghetti ME, Turner R, Gambini A, Visconti P, Perez-Martinez S, Gervasi MG. 2025. Comparative study of the effects of sperm energy restriction and recovery (SER) treatment on bovine and equine cryopreserved sperm. Gordon Research Conference and Gordon Research Seminar, Fertilization & Activation of Development; July 2025; Holderness, NH, USA. Poster presentation.
• Batey IE, Fioravanti P, Hill KS, Payton RR, Gan H, Zhao Y, Schrick FN, O’Neil MA, Edwards JL. 2025. Higher estrous associated temperatures (HEAT): relationship with different estrous active behaviors. 58th Annual Meeting of the Society for the Study of Reproduction; July 29–August 1, 2025; Washington, DC, USA.
• Bishop CV, Bowne S, Weiss T, Hanna C, Estill C, Ting A. 2025. Ovine model of whole ovary vitrification for cryopreservation: ovarian function post-transplantation. 58th Annual Meeting of the Society for the Study of Reproduction; July 31, 2025; Washington, DC, USA. Invited presentation.
• Bishop CV, Stormshak F. 2025. Mechanisms of luteal formation and maintenance. Advances in Reproductive Physiology: Unraveling the High Fertility Cycle in Cattle Symposium, ASAS-CSAS Annual Meeting; July 9, 2025; Hollywood, FL, USA. Invited presentation.
• Bonomo ZL, Devkota I, Fuego DM, Allon MA, Dozortsev D, Tralik B, Marbrey MW, Fu X, Simintiras CA. 2025. Cannabinoid exposure duration differentially alters human endometrial epithelial organoid behavior. Society for the Study of Reproduction Annual Meeting; July 2025; Washington, DC, USA. Oral and poster presentation.
• Branco LCS, Henning J, VanWye G, Ricardo K, Duit C, Thomas JM, Bilby TR, Rocha CC. 2025. Using automated monitoring devices to predict pregnancy by artificial insemination in grazing beef cows. American Society of Animal Science Annual Meeting; Hollywood, FL, USA.
• Brockman M, Rishi JK, Timme K, Keating AF. 2025. Impact of obesity on basal hepatic abundance of TLR4 and AOAH and the response to dimethylbenz[a]anthracene exposure in female mice. Society for the Study of Reproduction Annual Meeting.
• Chitwood BM, Hessock EA, Edwards JL, Payton RR, Campagna SR, Schrick FN, Moorey SE. 2025. Follicular fluid insulin, glucose, and their correlation with TCA cycle and purine metabolites are positively related to bovine preovulatory follicle maturity. 58th Annual Meeting of the Society for the Study of Reproduction; July 29–August 1, 2025; Washington, DC, USA.
• DeCastro LD, Stenhouse C, Hughes CHK. 2025. Metabolomic signatures of ovarian development in cattle. 58th Annual Meeting of the Society for the Study of Reproduction; July 29–August 1, 2025; Washington, DC, USA. Poster presentation. USDA NIFA Merit Award winner.
• Devkota I, Bonomo ZL, Fuego DM, Loux SC, Simintiras CA. 2025. Hormonal modulation of bovine endometrial epithelial organoids. Society for the Study of Reproduction Annual Meeting; July 2025; Washington, DC, USA. Poster presentation.
• Fuego DM, Devkota I, Bonomo ZL, Dondeti MF, Vourekas AV, Simintiras CA. 2025. Cannabinoid exposure alters oviduct fluid composition in vitro with potential implications for embryo epigenetic reprogramming. Society for the Study of Reproduction Annual Meeting; July 2025; Washington, DC, USA. Poster presentation.
• Folchini NP, Saad M, Kidwell EG, Bonacim PM, Ehresmann LX, Domingues RR, Kleinhenz M, Pohler K, Garcia-Guerra A. 2025. Functional role of prostaglandin F2α in luteal regression after induced pregnancy loss in cattle. Animal Reproduction 22(3).
• Ferst JG, da Silveira JC, Simintiras CA, Lonergan P. 2025. Increased negative energy balance alters the metabolic signature of uterine fluid extracellular vesicles and influences embryo metabolism in vitro. Brazilian Embryo Technology Society Annual Meeting; August 2025; Costa do Sauípe, Bahia, Brazil. Poster presentation.
• Fioravanti P, Moorey SE, Batey IE, Mills MD, Pollock AB, Hill KS, O’Neil MA, Payton RR, Hessock EA, Schrick FN, Edwards JL. 2025. Advanced preovulatory follicle progression associated with higher estrous-associated temperatures (HEAT) and impacted by estrous active behaviors. 58th Annual Meeting of the Society for the Study of Reproduction; July 29–August 1, 2025; Washington, DC, USA.
• Fioravanti P, Moorey SE, Batey IE, Mills M, Hill KS, Pollock AB, Payton RR, Hessock EA, Schrick FN, Campagna SR, O’Neil MA, Edwards JL. 2025. Impact of higher estrus-associated temperatures (H.E.A.T.) and mounting activity on the periovulatory follicular fluid metabolome in beef heifers. International Embryo Technology Society Annual Meeting; January 18–22, 2025; Fort Worth, TX, USA.
• Grote CE, Shofner I, Keller A, Timme K, Keating AF. 2025. Green eggs and ham – Development of a novel system for primordial follicle evaluation in the porcine ovary. Society for the Study of Reproduction Annual Meeting.
• González-Alvarez ME, Timme K, Keating AF. 2025. [No title provided]. Society for the Study of Reproduction Annual Meeting.
• M. Koppelman, J. P. Fulton, S. Khanal, A. Garcia-Guerra, T. Berger-Wolf. 2025. Utilizing image-based artificial intelligence for grading bovine cumulus-oocyte complexes. Animal – Science Proceedings 16(4):722–723. doi:10.1016/j.anscip.2025.08.279. Peer-reviewed.
• Haffner CJ, González-Alvarez ME, Timme K, Keating AF. 2025. Altered hepatic chemical biotransformation mRNA and protein abundance in prepubescent lean and obese female mice during exposure to dimethylbenz[a] Society of Toxicology Annual Meeting.
• Haine S, Glasper C, Do L, Weismann M, Murphy E, Przygrodzka E. 2025. Protein kinase A (PKA)-stimulated glucose metabolism is essential for granulosa cell function. ANSC Annual Symposium; May 2025; College Park, MD, USA.
• Henning J, Branco LCS, Diggs A, Allegro L, Griffin P, Silva AG, Bilby TR, Nickell J, Thomas JM, Rocha CC. 2025. Defining the interval from estrus to ovulation when using SenseHub accelerometers for estrus detection in grazing beef heifers. Applied Reproductive Strategies in Beef Cattle Conference; North Platte, NE, USA.
• Henning J, Branco LCS, VanWye G, Brown M, Duit C, Thomas JM, Martins T, Rocha CC. 2025. Using anogenital distance, a prenatal marker of androgen exposure, to predict pregnancy by artificial insemination in yearling beef heifers. Perinatal Biology Symposium, American Society of Animal Science; Snowmass, CO, USA.
• Henning J, Branco LCS, VanWye G, Brown M, Duit C, Thomas JM, Martins T, Rocha CC. 2025. Using anogenital distance at pre-breeding examination to predict pregnancy by artificial insemination in yearling beef heifers. American Society of Animal Science Annual Meeting; Hollywood, FL, USA.
• Henning J, Branco LCS, VanWye G, Ricardo K, Duit C, Thomas JM, Bilby TR, Rocha CC. 2025. Using automated monitoring devices to predict pregnancy by artificial insemination in grazing beef cows. DAREC Symposium; Columbia, MO, USA.
• Heredia D, Dias Zucoloto JC, Teixeira Zucoloto W, Dias Zucoloto T, Infante J, Luchini D, Gonella-Diaza A, Bertam C. 2025. Rumen-protected methionine modulates plasma and follicular fluid metabolites in beef cows. International Embryo Technology Society Annual Meeting. Reproduction, Fertility, and Development
• Hessock EA, Stokes AE, Clark HM, Edwards JL, Payton RR, Beever JE, Freeman TF, Schrick FN, Moorey SE. 2025. Transcript profiles of blastocysts produced from oocytes matured in fluid from preovulatory follicles of varied maturity. International Embryo Technology Society Annual Meeting; January 18–22, 2025; Fort Worth, TX, USA.
• Hill KS, Fioravanti P, Batey IE, Payton RR, O’Neil MA, Schrick FN, Menegatti Zoca S, Moorey SE, Edwards JL. 2025. Relationship between standing estrus at fixed-time artificial insemination with day 11 progesterone levels in suckled Bos taurus beef cattle. 58th Annual Meeting of the Society for the Study of Reproduction; July 29–August 1, 2025; Washington, DC, USA.
• Hill KS, Edwards JL, Payton RR, Schrick FN, Campagna SR, Hessock EA, Moorey SE. 2025. Relationships of circulating and preovulatory follicular fluid hydrogen peroxide level with body condition score and metabolome profiles in lactating beef cows. International Embryo Technology Society Annual Meeting; January 18–22, 2025; Fort Worth, TX, USA.
• Hoorn QA, Simintiras CA, Kenny DA, Fair S, Lonergan P. 2025. Sperm metabolomic profiles reflect bull fertility status. Association of Embryo Technology in Europe Annual Meeting; September 2025; Cork, Ireland. Poster presentation.
• Hughes CHK. 2025. Luteolysis at single cell resolution: luteal cell–immune cell interactions. Society for the Study of Reproduction Annual Meeting; July 28–August 1, 2025; Washington, DC, USA.
• Hughes CHK. 2025. SF-1 regulates ovarian follicle formation and development. ENDO 2025; July 12–15, 2025; San Francisco, CA, USA.
• Hughes CHK. 2025. Novel mechanisms conferring resistance to regression during luteal development and pregnancy. American Dairy Science Association Annual Meeting; June 22–25, 2025; Louisville, KY, USA.
• Hughes-Brown JM, Pate JL, Hughes CHK. 2025. Modulation of apoptotic mechanisms in developing bovine corpora lutea may regulate susceptibility to luteolysis in response to PGF2α. 58th Annual Meeting of the Society for the Study of Reproduction; July 29–August 1, 2025; Washington, DC, USA. Oral presentation. Trainee travel award winner.
• Hutchings D, Elder D, Tsang PC. 2026. The phytoestrogen equol dysregulates the function of the bovine corpus luteum. Society for the Study of Reproduction Annual Meeting; July 2026; Indianapolis, IN, USA. Poster presentation.
• Ibadin NO, Diaz FJ. 2025. Differential effects of potassium channel modulators on mouse sperm motility and function. Society for the Study of Reproduction Annual Meeting; July 29–August 5, 2025; Washington, DC, USA.
• Infante J, Wang J, Heredia D, Venturini D, Lopez-Duarte MC, Yu H, Gonella A. 2025. Predicting active and non-active corpus luteum in early pregnancy using computer vision and machine learning on Doppler ultrasound images in beef cattle. Journal of Animal Science 103(Suppl 3):366.
• Kennedy J, Peterson KD, Chitwood BM, Lonas AB, Young MA, Payton RR, Poudel SP, Behura SK, Oliver MA, Strickland LG, Prado TM, Edwards JL, Charpigny G, Spencer TE, Mathew DJ. 2025. Impact of early pregnancy on the maternal anterior pituitary transcriptome in cattle. 58th Annual Meeting of the Society for the Study of Reproduction; July 29–August 1, 2025; Washington, DC, USA.
• Klabnik JL, Beever JE, Payton RR, Lamour KH, Schrick FN, Edwards JL. 2025. A step toward understanding direct impacts of a higher estrus-associated temperature (HEAT): transcript level changes in cumulus-oocyte complexes directly exposed to acute elevated temperature. International Embryo Technology Society Annual Meeting; January 18–22, 2025; Fort Worth, TX, USA.
• Koppelman GM, Fulton JP, Khanal S, Garcia-Guerra A, Berger-Wolf T. 2025. Utilizing image-based artificial intelligence for grading bovine cumulus-oocyte complexes. Animal – Science Proceedings 16(4):722–723. doi:10.1016/j.anscip.2025.08.279. Peer-reviewed.
• Kuzniar MA, Edwards JL, Payton RR, Campagna SR, Sarumi Q, Hessock EA, Schrick FN, Moorey SE. 2025. Relationship between preovulatory follicle maturity and the follicular fluid metabolome. International Embryo Technology Society Annual Meeting; January 18–22, 2025; Fort Worth, TX, USA.
• Laplacette AL, Giordano JO. 2025. Combining early lactation automated estrus alerts and predictors of reproductive performance for grouping cows based on first service reproductive potential. Journal of Dairy Science 108(E-Suppl 1).
• Laplacette AL, Giordano JO. 2025. Patterns of behavioral parameters and performance outcomes in lactating cows with automated health alerts and no clinical signs of disease. Journal of Dairy Science 108(E-Suppl 1).
• Laplacette AL, Giordano JO. 2025. Predicting first service pregnancy outcome in lactating dairy cows with machine learning algorithms trained on behavioral, performance, and cow feature data. Journal of Dairy Science 108(E-Suppl 1).
• Lopez-Duarte MC, Heredia D, Venturini M, Infante J, Catussi B, Maderal A, Tarnonsky F, Gonella-Diaza A. 2025. Characterization of plasmatic extracellular vesicles during pregnancy in beef cattle. International Embryo Technology Society Annual Meeting. Reproduction, Fertility, and Development
• Moorey SE. 2025. Role of the preovulatory follicle in oocyte metabolism and competence for embryo development in cattle. American Dairy Science Association Annual Meeting; June 22–25, 2025; Louisville, KY, USA.
• Mostafa M, Tyree MF, Stenhouse C, Hughes CHK. 2025. Orphan nuclear receptor SF-1 as a potential novel regulator of ovarian reserve establishment in cattle. 58th Annual Meeting of the Society for the Study of Reproduction; July 29–August 1, 2025; Washington, DC, USA. Accepted presentation; withdrawn due to health of first author.
• Motta JCL, Carranza-Martin AC, Saad M, Rykaczewski C, Crist A, Folchini NP, Sala RV, Bond R, Ross PJ, Garcia-Guerra A. 2025. Association between circulating anti-Müllerian hormone at birth and puberty onset in Holstein heifers. Proceedings of the 58th Annual Meeting of the Society for the Study of Reproduction; July 29–August 1, 2025; Washington, DC, USA. P110.
• Motta JCL, Sala RV, Absalón-Medina VA, Fricke VC, Ross PJ, Moreno JF, Garcia-Guerra A. 2025. Ovarian stimulation with FSH in low AMH heifers increases ovarian response and oocyte developmental competence in a dose-limited manner. Reproduction, Fertility and Development 37(1). doi:10.1071/RDv37n1Ab209.
• Motta JCL, Garcia-Guerra A. 2025. Ovarian reserve and fertility: bridging basic science and reproductive management. Journal of Animal Science 103(Suppl 3):224–225. doi:10.1093/jas/skaf300.265.
• Saad M, Rykaczewski C, Edwards A, Ponte E, da Silva Junior FPJ, Domingues R, Garcia-Guerra A. 2025. Accessory corpora lutea in ewes: influence on fertility and CL maintenance. Proceedings of the 58th Annual Meeting of the Society for the Study of Reproduction; July 29–August 1, 2025; Washington, DC, USA. P246. Peer-reviewed.
• Oliver MA, Ealy AD. 2025. Interleukin-6 and leukemia inhibitory factor influence epiblast cell number and hypoblast migration in cultured, post-hatched bovine blastocysts. Society for the Study of Reproduction Annual Meeting; July 29–August 1, 2025; Washington, DC, USA.
• Payton RR, Moorey SE, Campagna SR, Schrick FN, Pohler KG, Edwards JL. 2025. Heat-induced hyperthermia after a GnRH-induced LH surge impacts circulating and periovulatory intrafollicular metabolites in lactating dairy cows. 58th Annual Meeting of the Society for the Study of Reproduction; July 29–August 1, 2025; Washington, DC, USA.
• Payton RR, Moorey SE, Campagna SR, Schrick FN, Pohler KG, Edwards JL. 2025. Heat-induced hyperthermia impacts the periovulatory follicular fluid metabolome in lactating dairy cows. International Embryo Technology Society Annual Meeting; January 18–22, 2025; Fort Worth, TX, USA.
• Peterson KD, Kolape J, Mathew DJ. 2025. Evidence of endometrial binucleate cells in cattle: a product of monoculture or a new endometrial epithelial phenotype? 58th Annual Meeting of the Society for the Study of Reproduction; July 29–August 1, 2025; Washington, DC, USA.
• Przygrodzka E, Haine S, Do L, Weisman M, Glasper C, Murphy E. 2025. Protein kinase A (PKA)-mediated chromatin accessibility and transcriptomic alterations in granulosa cells. Society for the Study of Reproduction Annual Meeting; July 2025; Washington, DC, USA.
• Roa-Vidal P, Markway M, Moraes JGN, Poock S, Matthew LC, Patterson AL. 2025. Beyond the lesions: adenomyosis alters uterine gene expression at a global scale in the bovine model. Gilbert S. Greenwald 22nd Annual Symposium on Reproductive and Developmental Sciences; October 16–17, 2025; Kansas City, KS, USA. Poster presentation.
• Roa-Vidal P, Markway M, Moraes JGN, Matthew LC, Patterson AL. 2025. Implementing the dairy cow as a novel study model to uncover cell migration pathways dysregulated in adenomyosis. Society for Reproductive Investigation 72nd Annual Meeting; March 25–29, 2025; Charlotte, NC, USA. Poster presentation.
• Roberts SR, Hessock EA, Lonas AB, Campagna SR, Moorey SE, Zoca SM. 2025. Association between sperm metabolites and field fertility in beef bulls. International Embryo Technology Society Annual Meeting; January 18–22, 2025; Fort Worth, TX, USA.
• Roth E, Pearl S, Sand J, Bello T, Przygrodzka E. 2025. PKA signaling drives metabolic and epigenetic reprogramming during granulosa cell luteinization. Honors Program, University of Maryland.
• Sellmer Ramos I, Caldeira MO, Moraes JGN, Poock SE, Lucy MC, Patterson AL. 2025. Investigating the phenotype of putative stem/progenitor epithelia established at the endometrial stratum basalis of the bovine uterus. Gilbert S. Greenwald 22nd Annual Symposium on Reproductive and Developmental Sciences; October 16–17, 2025; Kansas City, KS, USA. Poster presentation.
• Sellmer Ramos I, Caldeira MO, Poock SE, Moraes JGN, Lucy MC, Patterson AL. 2025. Characterization of the endometrial epithelium of postpartum dairy cows using a multimodal transcriptomic approach to investigate disease programming of fertility. American Society of Animal Science Midwest Section Annual Meeting; March 9–12, 2025; Omaha, NE, USA. Poster presentation.
• Timme K, González-Alvarez ME, Keating AF. 2025. Inhibition of PI3K altered the ovarian response to DMBA exposure. Society of Toxicology Annual Meeting.
• Tourzani DA, Guo S, Visconti P, Gervasi MG. 2025. Sperm energy restriction and recovery (SER) prior to IVF alters early embryo development and implantation competence. Gordon Research Conference and Gordon Research Seminar, Fertilization & Activation of Development; July 2025; Holderness, NH, USA. Poster presentation.
• Venturini M, de Agostini Losano J, Heredia D, Lopez-Duarte MC, Infante J, Podversich F, Tarnonsky F, Gonella A, McGraw M, Muhammad S, Guertin J, Poliakiwski B, Smith D, Pohler K, DiLorenzo N, Daigneault B. 2025. Relationship between reproductive parameters and residual feed intake in yearling beef bulls. American Society of Animal Science Southern Section Annual Meeting; 2025. Journal of Animal Science 103(Suppl 2):138.

Theses/Dissertations (2025)

• Batey IE. Estrous active behaviors and other factors related to higher estrous-associated temperatures in beef cattle. MS thesis.
• Christian H. Obesity modulates basal and 7,12-dimethylbenz[a]anthracene-induced hepatic inflammatory signaling and xenobiotic biotransformation pathways. MS thesis.
• Crist A. Enhancing ovulatory response and fertility in beef cows: presynchronization approaches using progesterone and prostaglandin F2α. MS thesis, The Ohio State University.
• Fuego DM. Oviduct fluid metabolic regulation of embryonic genome methylation. MS thesis.
• Gabel A. Zinc-dependent signaling and ZIP9 function in mammalian mural granulosa cells. PhD dissertation.
• Heredia D. Rumen-protected methionine supplementation during the periconceptional period in beef cattle: impacts on postnatal development and offspring performance. MS thesis.
• Hughes-Brown J. A multiomics approach to characterizing the acquisition of luteolytic capacity in the bovine corpus luteum. MS thesis.
• Keane JA. Enhancing bovine in vitro embryo production: effects of immunomodulatory and antioxidant supplements during oocyte maturation. PhD dissertation.
• Lopez Duarte MC. Cell-free DNA and extracellular vesicles as indicators of reproductive status in beef cows. MS thesis.
• Oliver MA. Investigating the actions of interleukin-6 family members on post-hatching blastocyst development in cattle. PhD dissertation.
• Roberts SR. Association between sperm metabolites and field fertility in Angus bulls. MS thesis.
• Venturini M. Residual feed intake in beef cattle: omics technologies to identify biomarkers and reproductive-related traits. MS thesis.