SAES-422 Multistate Research Activity Accomplishments Report

Sections

Status: Approved

Basic Information

Participants

Amy Desaulniers, University of Nebraska Jean Feugang, Mississippi State University Jay Johnson, University of Missouri Karl Kerns, Iowa State University Rob Knox, University of Illinois Jeremy Miles, USDA-USMARC Dave Miller, University of Illinois Kayla Mills, USDA-BARC Lea Rempel, USDA-USMARC Tim Safranski, University of Missouri Taw Scaff, North Dakota State University Claire Stenhouse, Pennsylvania State University Brett White, University of Nebraska Michal Zigo, University of Missouri

The 2025 meeting was held virtually.  It was called to order by Chair Brett White of the University of Nebraska at 10:00 am on May 20. The first order of business was a USDA-NIFA report from our representatives, Drs. Mark Mirando and Kamilah Grant. They provided updated information about USDA-NIFA and Capacity Funding (Formula Funds). After this update, station reports were provided from USDA-BARC, Missouri, Illinois, and Nebraska (Brett White). The meeting continued on May 21 with the Business meeting (minutes below), followed by station reports from USDA-MARC, Mississippi State, and Iowa State.

The Business meeting was called to order by Brett White at 9:00 AM on May 21. The first order of business was to decide on next year’s meeting. 2026 would be a year for the symposium that is associated with the annual NCERA57 meeting. There was discussion about having the symposium and meeting at World Pork Expo in Des Moines, IA, scheduling the symposium in the afternoon (June 3rd or 4th) or perhaps just before or just after Pork Expo. But there was some concern that many attending Pork Expo would spend only one day there and may not attend the symposium. Another possibility was to have the symposium as part of Iowa Swine Day at the Iowa Pork Industry Center on June 26 in Ames, IA. It was decided to inquire if there was interest by the potential hosts. The potential topics for the symposium that were mentioned were New Technologies in Swine Reproduction such as Gene Editing, Robotics, Artificial Intelligence, Precision Reproductive Management, and new developments in Piglet Survival. A committee of Karl Kerns, Brett White, Tim Safranski, and Jeremy Miles was appointed. Karl Kerns was elected unanimously as the new secretary.

Accomplishments

  • In support of Objectives 1 and 4, investigators characterized the lipidome and metabolome of boar sperm cells before and after in-vitro capacitation (USDA-ARS-BARC and Iowa State).
  • In support of Objectives 1 and 4, researchers developed and validated a new sample processing technique for lipidomic analysis better suited for commercial boar studs (USDA-ARS-BARC and Purdue).
  • In support of Objectives 1 and 4, researchers evaluated the lipidomic profile of the ejaculate at collection from boars of different ages (USDA-ARS-BARC and Purdue).
  • In support of Objectives 1, 3, and 4, investigators have carried out an experiment to test the effects on the sperm proteome of a mild acute increase in scrotal temperature in pigs. Over 800 proteins were identified from porcine sperm. The abundance of 4-20 proteins was altered at various times following mild scrotal hyperthermia, including proteins with a potential function in the recovery from insult. Some were produced in the testis and others in the epididymis, an unexpected result. Four proteins were studied in more detail. All four are found in the mitochondria as well as other locations in sperm collected from the testis, caput and cauda epididymis and are also found in extracellular vesicles from the caput and cauda epididymis. The extracellular vesicles from the epididymis fuse with caput epididymal sperm in vitro and could supplement the amount of these 4 proteins during sperm passage. Therefore, an increase in scrotal temperature appears to increase the secretion of extracellular vesicles from the epididymis that can fuse with sperm and increase the abundance of several consensus mitochondrial proteins (Illinois and Wisconsin).
  • In support of Objectives 1, 3, and 4, using metabolomics, researchers discovered that binding to oviduct glycans extends sperm lifespan by slowing the production of a ubiquinone precursor, reducing the activity of the electron transport chain, reducing fumarate, an intermediate in the Krebs cycle, and reducing oxidative phosphorylation. This resulted in reduced production of toxic reactive oxygen species (Illinois and Missouri).
  • In support of Objectives 1, 3, and 4, it was discovered that a protein called PKDREJ has affinity for Lewis X, an oviduct trisaccharide. Pigs engineered to have sperm deficient in PKDREJ were infertile, although their sperm had normal viability, motility and morphology. When tested using a glycan array, PKDREJ-deficient sperm were less able to bind specific glycans, some of which are found in the oviduct. Additional experiments showed that the overall protein content of PKDREJ-deficient sperm was unchanged. If sperm were surgically inseminated into the oviduct, or in vitro fertilization was performed, PKDREJ-deficient sperm produced no embryos. Thus, PKDREJ deficiency resulted in sperm that were unable to fertilize oocytes in vivo or in vitro (Illinois and Missouri).
  • In support of Objectives 1, 3, and 4, investigators discovered that the porcine oviduct epithelium expresses 5 of the immunosuppressive Siglec proteins and only 2 immunostimulatory Siglec proteins, more immunosuppressive Siglec proteins than found in the uterus. This corresponds to the strong innate immune response against sperm in the uterus but the minimal immune response in the oviduct. Sperm also contain sialoglycans that are high-affinity Siglec ligands. By modulating the abundance of sialic acid on sperm, and, correspondingly, the affinity for Siglecs, the immune response of oviduct cell spheroids to sperm was perturbed, suggesting that Siglecs on the oviduct can regulate the response of the oviduct to sperm (Illinois and Missouri).
  • In support of Objective 1 and 2, investigators reported on development and function of GnRH-II and its receptor in both male and female swine using knockdown technology (USMARC and University of Nebraska Lincoln).
  • In support of Objectives 1, 3 and 4, investigators identified target candidates of the ubiquitin proteasome during porcine sperm capacitation that can be modulated to improve semen extension for the improvement of artificial insemination outcomes (Missouri and Iowa).
  • In support of Objective 1 and 3, investigators identified porcine sperm fertility biomarkers based on sperm phenotyping (Missouri and Iowa).
  • In support of Objective 3, investigators revised in vitro sperm capacitation approaches (Missouri and Iowa).
  • In support of Objective 3 and 4, investigators clarified the paternal contribution to the mammalian oocyte and its potential for sperm fertility biomarkers (Missouri and Iowa).
  • In support of Objective 1, 2 and 4, investigators reported on development and function of GnRH-II and its receptor in both male and female swine using knockdown technology (USMARC and University of Nebraska Lincoln).
  • In support of Objective 2, researchers indicated that GnRH-II receptor knockdown preovulatory follicles possessed fewer, hypertrophic theca cells and a similar number of hypotrophic granulosa cells than littermate control follicles (University of Nebraska-Lincoln and USMARC).
  • In support of Objective 4, investigators reported that the proteome of theca cells from preovulatory follicles of GnRHR-II KD gilts was significantly altered compared to littermate control gilts, whereas the proteome of granulosa cells did not differ between swine lines (University of Nebraska-Lincoln and USMARC).
  • In support of Objective 2 and 4, researchers have utilized precision technology tools and genomics to improve piglet and sow performance (USMARC and University of Nebraska-Lincoln).
  • In support of Objective 1 and 4, investigators determined that neonatal intrauterine growth restricted boars (IUGR) boars have distinct cellular and molecular alterations within the testis that may drive poor reproductive function in adulthood. (University of Nebraska-Lincoln and University of Missouri).
  • In support of Objective 1 and 4, researchers reported that arginine supplementation of the diet improved semen volume and total sperm counts, as well as significantly altered seminal plasma proteins. (Mississippi State University and University of Nebraska-Lincoln).
  • In support of Objective 4, investigators revealed that maternal atrazine exposure in the water throughout gestation disrupted progesterone concentrations in umbilical cord blood of exposed piglet offspring (University of Illinois and University of Nebraska-Lincoln, University of Illinois, and Iowa State University).
  • In support of Objective 4, researchers indicated that testosterone concentrations were greater in neonatal males provided sow milk depleted of bioactive extracellular vesicles compared with those given milk replacer + bioactive extracellular vesicles or normal sow milk (University of Nebraska-Lincoln and USMARC).
  • In support of Objective 5- member of the Planning Committee for the SowBridge program, an extension program targeted to translating science into usable information for swine farms (North Dakota and Iowa).
  • In support of Objective 6- Provided an Extension webinar on semen processing, collection, and use in artificial insemination.
  • In support of Objective 4, we have discovered that larger embryos secreted more estradiol and IL1beta and male embryos secreted more estradiol than female embryos
  • In support of Objective 6, a survey of swine producers was administered to identify current health, reproductive, feed source and management problems. Four producers also volunteered to allow the collection of serum samples for vitamin A and D analysis (Pennsylvania and Texas).

Impacts

  1. • Characterizing the lipidome and metabolome of boar sperm cells following capacitation revealed novel insights into metabolic shifts and regulatory effects of zinc, underscoring the importance of metabolic changes and could improve artificial insemination techniques, breeding strategies, or fertility treatment modalities (Objectives 1 and 4).
  2. • The alternative lipidomic sample processing technique allows ejaculates to be processed at room temperature rather than needing dry ice or liquid nitrogen, increasing the feasibility of lipidomic screening intended for boar fertility phenotyping (Objectives 1 and 4)..
  3. • Despite older chronological age some boars appear less mature according to lipidome signatures and suggests not all boars mature or peak in their semen production at the same rate, identifying another potential area for selection improvement (Objective 1).
  4. • Because elevated scrotal temperature also affects the epididymis in addition to the testis, the epididymis should also be considered a target for cooling to maintain fertility (Objectives 1 and 4).
  5. • An understanding of how sperm lifespan is prolonged in the sperm reservoir before fertilization, both by changes in sperm metabolism and possible suppression of the innate immune response was developed. This knowledge may lead to increased sperm fertile life in the female reproductive tract (Objectives 1 and 4).
  6. • A potential oviduct glycan receptor may be used as a biomarker for sperm fertility (Objectives 1 and 4).
  7. • Precision technology tools and genomics that can be used to improve piglet and sow performance (Objectives 2 and 4).
  8. • The improved understanding of porcine testis biology may be used to improve boar reproductive performance (Objectives 1 and 4).
  9. • Fertility outcomes in gilts may be increased by modulating GnRH-II and its receptor (Objective 2 and 4).
  10. • Integration of kisspeptin knockout swine into production systems could reduce the cost associated with castration or castration alternatives, as well as help ensure a quality product is supplied to the consumer (Objective 1).
  11. • Milk extracellular vesicles (and their microRNA cargo) are unexploited mediators of testis development (Objective 4).
  12. • Environmentally relevant maternal atrazine exposure disrupts progesterone levels in neonatal piglets. Minimizing exposure may improve boar performance (Objective 4).
  13. • Even low-dose perinatal atrazine exposure of female piglets may impair ovarian function and long-term reproductive health. Minimizing exposure may improve gilt/sow performance (Objective 4).
  14. • By improving the understanding of the management practices, especially in relation to nutrient availability and reproductive management, it may be possible to improve pregnancy outcomes in small ruminant production systems (Objective 1).
  15. NIH, R01HD095841, Accumulation, Storage, and Release of Sperm in the Oviduct, $1,982,500, 7/1/2023 - 6/30/2028, Primary Investigator, David Miller, Collaborator, Randy Prather and Kiho Lee, University of Missouri.
  16. USDA-AFRI-NIFA Grant #2021-67015-34416, $500,000, Jul 1, 2021 – June 30, 2026, “Influence of Maternal and Embryonic Extracellular Vesicles on the Initiation of Porcine Conceptus Elongation” Pannier1, A.K. (PD), Miles2, J.R. (Co-PD), Rempel2, L.A. (1University of Nebraska, 2USDA, ARS, USMARC).
  17. USDA-AFRI-NIFA Grant #2023-67015-39451, $650,000, May 1, 2023 – May 1, 2026, “Enhancing boar fertility in the face of climate change through the mitigation of in utero heat stress” Desaulniers1, A.T. (PD), Lents2, C., White1, B., Brito3, L, Johnson4, J. (1University of Nebraska; 2USDA, ARS, USMARC, 3Purdue University; 4University of Missouri).
  18. USDA-AFRI-NIFA Grant #2022-08916, $ 999,618, May 1, 2023 – May 1, 2027, “Identifying AMR gene reservoirs and bacterial host-AMR gene associations to identify bacterial host range of AMR genes in swine production systems” Fernando1, S., Burkey1, T., Miller1, P., Schmidt2, A., Anderson2, C., Oliver3, W., Rempel3, L., Wells3, J. (1University of Nebraska; 2Iowa State University; 3USDA, ARS, USMARC).
  19. USDA-AFRI-NIFA Grant #2023-67015-39451, $650,000, May 1, 2023 – May 1, 2026, “Enhancing boar fertility in the face of climate change through the mitigation of in utero heat stress” Desaulniers1, A.T. (PD), Lents2, C., White1, B., Brito3, L, Johnson4, J. (1University of Nebraska; 2USDA, ARS, USMARC, 3Purdue University; 4University of Missouri).

Publications

Peer-reviewed Journals

  1. Ahern, D.F., Martins, K., Florez, J.M., Ross, C.E., Huisman, A., Cushman, R.A., Shuping, S.L., Nestor, C.C., Desaulniers, A.T., White, B.R., Sonstegard, T.S., Lents, C.A. 2024. Development of KISS1 knockout pigs is characterized by hypogonadotropic hypogonadism, normal growth, and reduced skatole. Biology of Reproduction. 111(5):1082-1096. https://doi.org/10.1093/biolre/ioae140.
  2. Ahern, D.F., Martins, K., Florez, J.M., Ross, C.E., Huisman, A., Cushman, R.A., Shuping, S.L., Nestor, C.C., Desaulniers, A.T., White, B.R., Sonstegard, T.S., Lents, C.A. 2024. Development of KISS1 knockout pigs is characterized by hypogonadotropic hypogonadism, normal growth, and reduced skatole. Biology of Reproduction. 111(5):1082-1096. https://doi.org/10.1093/biolre/ioae140.
  3. Bery, S., Brown-Brandl, T.M., Rohrer, G.A., Sharma, S., Leonard, S.M. 2024. Impact of crate design, number of heat lamps, and lying posture on the occurrence of shoulder lesions in sows. Biosystems Engineering. 247:249-256. https://doi.org/10.1016/j.biosystemseng.2024.09.017.
  4. Desaulniers, A.T., Cederberg, R.A., Lents, C.A., White, B.R. 2024. Knockdown of gonadotropin-releasing hormone II receptor impairs ovulation rate, corpus luteum development, and progesterone production in gilts. Animals. 14. Article 2350. https://doi.org/10.3390/ani14162350.
  5. Desaulniers, A.T., Cederberg, R.A., Lents, C.A., White, B.R. 2024. Knockdown of gonadotropin-releasing hormone II receptor impairs ovulation rate, corpus luteum development, and progesterone production in gilts. Animals (Basel). 14(16):2350. https://doi.org/10.3390/ani14162350.
  6. Desaulniers, A.T., Ross, C.E., Cederberg, R.A., Lovercamp, K.W., Lents, C.A., White, B.R. 2024. Gonadotropin-releasing hormone II and its receptor regulate motility, morphology, and kinematics of porcine spermatozoa in vitro. General and Comparative Endocrinology. 361. Article 114653. https://doi.org/10.1016/j.ygcen.2024.114653.
  7. Desaulniers, A.T., Ross, C.E., Cederberg, R.A., Lovercamp, K.W., Lents, C.A., White, B.R. 2024. Gonadotropin-releasing hormone II and its receptor regulate motility, morphology, and kinematics of porcine spermatozoa in vitro. General and Comparative Endocrinology. 361:114653.https://doi.org/10.1016/j.ygcen.2024.114653.
  8. Lopez AN, Olivarez MA, Stenhouse C, Moses RM, Newton MG, Sah N, Seo H, Cain J, Lefevre C, Ross A, Ryan P, Wiegert JG, et al. Effects of dietary supplementation of creatine on fetal development in gilts at d 60 and d 90 of gestation. J Anim Sci Biotechnol 2025; 16.
  9. Mills K.M., Minton A.M., Ferreira C.R. (2024). Adapting lipidomic sample processing methods for boars housed in commercial settings. Translational Animal Science. txae139, https://doi.org/10.1093/tas/txae139.
  10. Newton MG, Lopez AN, Stenhouse C, Hissen KL, Connolly ED, Li X, Zhou L, Wu G, Foxworth WB, Bazer FW. Impact of dietary supplementation of L-citrulline to meat goats during gestation on reproductive performance. J Anim Sci Biotechnol 2025; 16.
  11. Oladejo, E.O., Gruhot, T.R., Park, S., Ishak, G.M., Mote, B.E., Liao, S.F., Feugang, J.M. 2025. Dietary arginine supplementation modulates the proteome of boar seminal plasma. Animals (Basel). 15(4):555. https://www.mdpi.com/2076-2615/15/4/555.
  12. Ostrand, L.M., Rempel, L.A., Keel, B.N., Snelling, W.M., Schmidt, T.B., Psota, E.T., Mote, B.E., Rohrer, G.A. 2025. Genomic analysis of mobility measures on 5-month-old gilts associated with structural soundness. Journal of Animal Science. 103. Article skaf001. https://doi.org/10.1093/jas/skaf001.
  13. Ostrand, L.M., Rempel, L.A., Keel, B.N., Snelling, W.M., Schmidt, T.B., Psota, E.T., Mote, B.E., Rohrer, G.A. 2025. Genomic analysis of mobility measures on 5-month-old gilts associated with structural soundness. Journal of Animal Science. 103:skaf001. https://doi.org/10.1093/jas/skaf001.
  14. Paudel, S., Brown-Brandl, T.M., Rohrer, G., Sharma, S.R. 2025. Deep learning algorithms to identify individual finishing pigs using 3D data. Biosystems Engineering. 255. Article 104143. https://doi.org/10.1016/j.biosystemseng.2025.104143.
  15. Rahman, Md T., Brown-Brandl, T.M., Rohrer, G.A., Sharma, S.R., Shi, Y. 2024. Classification of sow postures using convolutional neural network and depth images. In: Proceedings of the American Society of Agricultural and Biological Engineers International (ASABE), July 28-31, 2024, Anaheim, CA. Paper 2401533. https://doi.org/10.13031/aim.202401533.
  16. Reis TRL, Lopes-Ferreira JV, Guerra KB, da Silva LAB, Stenhouse C, Ashworth CJ, Bloise E, Chiarini-Garcia H, Almeida FRCL de. The structure of the porcine uterine-conceptus interface is associated with gestational day, fetal size and sex. Domest Anim Endocrinol 2025; 90.
  17. Rivera-Colon, I., Harkow, K., Cole, R., O'Meally, R., Garrett, W.M., Xiong, W., Oliver, W.T., Wells, J., Summers, K.L., Chhetri, N., Postnikova, O.A., Rempel, L.A., Crouse, M.S., Neville, B.W., Davies, C.L. 2025. A metaproteomic analysis of the piglet fecal microbiome during the weaning transition. Frontiers in Microbiology. 16. Article 1504433. https://doi.org/10.3389/fmicb.2025.1504433.
  18. Sah N, Stenhouse C, Halloran KM, Moses RM, Newton MG, Seo H, Cain JW, Lefevre CM, Johnson GA, Wu G, Bazer FW. Effect of gestational age and fetal sex on metabolism of creatine by uteri, placentae, and fetuses of pigs. Biol Reprod
  19. Stenhouse C, Donadeu FX, Almeida FRCL. Editorial: Intrauterine growth restriction: screening and outcomes. Front Physiol 2024; 15.
  20. Stenhouse C, Halloran KM, Hoskins EC, Moses RM, Wu G, Seo H, Johnson GA, Suva LJ, Gaddy D, Bazer FW. Progesterone regulates tissue non-specific alkaline phosphatase (TNSALP) expression and activity in ovine utero-placental tissues. J Anim Sci Biotechnol 2024; 15:1–14.
  21. Stenhouse, C, Halloran, KM, Hoskins, EC, Moses, RM, Newton, MG, Sah, N, Wolpo, YE, Tyree, MF, Seo, H, Johnson, GA, Wu, G, Bazer, FW. Characterization of the Expression of XPR1 in Ovine Utero-Placental Tissues. (2025) Reproduction. In Press. doi: 10.1530/REP-25-0072
  22. Tyree MF, Stenhouse C. Exogenous Progesterone Supplementation: A Strategy to Enhance Conceptus Development in Sheep and Pigs? Reproduction & Fertility 2025; 6.
  23. Weide T., Mills K.M., Shofner I., Breitzman M., Kerns K. (2024). Metabolic Shift in Porcine Spermatozoa during Sperm Capacitation-Induced Zinc Flux. International Journal of Molecular Sciences. 25(14):7919. https://doi.org/10.3390/ijms25147919.
  24. Zigo M, Kerns K, Sutovsky P.Methods Mol Biol. 2025;2954:229-240. doi: 10.1007/978-1-0716-4698-4_13.PMID: 40601279
  25. Zigo M, Netherton J, Zelenková N, Kerns K, Kraus V, Postlerová P, Baker M, Sutovsky P (2024) Bottom-up Approach to Deciphering the Targets of the Ubiquitin-Proteasome System in Porcine Sperm Capacitation. Sci Rep 14(1):200159.

 

Abstracts/Posters/Professional Presentations

  1. Mills K.M., A.M. Minton, C.R. Ferreira. (2024). Oral Communication 14 - Adapting sample processing methods for lipidomic analysis of ejaculates from commercial boars. Animal Reproduction Science. 278:107904.
  2. Doungkamchan K, Lucas C, Wells K, Molina L, Whitworth K, Prather R, Miller DJ. 2024. Knockout of PKDREJ, a candidate oviduct receptor on porcine sperm, reduces fertility assessed by competitive insemination. Annual Meeting of the Society for the Study of Reproduction. (selected for oral presentation).
  3. Doungkamchan K, Molina L, Lucas C, Govindasamy K, Wells K, Whitworth K, Prather R, Miller DJ. 2024. Knockout of PKDREJ, a candidate oviduct receptor on porcine sperm, results in male infertility. Symposium of the Upper Midwest Summit for Reproductive Sciences.
  4. Lents, C.A., Ahern, D.F., Wilson, K.E., Ross, C.E., Mills, G., Elsken, D.H., Florez, J.M., Martins, K., Beltramo, M., Sonstegard, T.S., Cushman, R.A., White, B.R. 2024. Serum concentrations of luteinizing hormone (LH) and follicle stimulating hormone (FSH) in KISS1 knockout gilts treated with neurokinin B, kisspeptin, and GnRH hormone analogs [abstract]. Society for the Study of Reproduction Annual Meeting. July 15-19, 2024, Dublin, Ireland. Focus Session 31.
  5. Rahman, Md T., Brown-Brandl, T.M., Rohrer, G.A., Sharma, S.R., Shi, Y. 2024. Classification of sow postures using convolutional neural network and depth images. In: Proceedings of the American Society of Agricultural and Biological Engineers International (ASABE), July 28-31, 2024, Anaheim, CA. Paper 2401533. https://doi.org/10.13031/aim.202401533.
  6. Rohrer, G.A., Ostrand, L., Rempel, L.A., Keel-Mercer, B.N., Schmidt, T.B., Mote, B.E. 2024. Genomic regions associated with electronic measures indicative of structural soundness in pigs [abstract]. American Society of Animal Science Annual Meeting. 102(3):24-25. https://doi.org/10.1093/jas/skae234.027.
  7. Sutovsky, P, Hamilton LE, Zigo M, Assumpção MEOA, Jones A, Tirpak F, Agca Y, Kerns K, Sutovsky M (2024) Biomarker-based human and animal sperm phenotyping: the good, the bad and the ugly. Biol Reprod 110(6):1135.
  8. Zigo M, Kerns K, Ahlering P, Sutovsky P. Proteins of Huntington’s Disease Pathway Participate in Pig Sperm Capacitation. Presented at the 57th Annual Meeting of the Society for Study of Reproduction, 16-19 July 2024 in Dublin, Ireland.
  9. Adeyanju, O., González-Alvarez, M.E., Antwi-Boasiako, C., Polivanov, L.J., Shelton, J., Nordell, N.E., Sillman, S.J., White, B.R., Laws, M.J., Raetzman, L., Flaws, J.A., Desaulniers, A.T., Keating, A.F. 2024. Female offspring ovarian chemical metabolism protein abundance is altered by in utero atrazine exposure [abstract]. Society for the Study of Reproduction Annual Meeting. July 15-19, 2024, Dublin, Ireland.
  10. Laws, M.J., Kramer, S., Weis, K., Polivanov, L.J., Shelton, J., Nordell, N.E., White, B.R., Sillman, S.J., Raetzman, L., Keating, A., Flaws, J.A., Desaulniers, A.T. 2024. Environmentally relevant maternal atrazine exposure leads to aberrant endocrine effects in piglets [abstract]. Society for the Study of Reproduction Annual Meeting. July 15-19, 2024, Dublin, Ireland.
  11. Lents, C.A., Ahern, D.F., Wilson, K.E., Ross, C.E., Mills, G., Elsken, D.H., Florez, J.M., Martins, K., Beltramo, M., Sonstegard, T.S., Cushman, R.A., White, B.R. 2024. Serum concentrations of luteinizing hormone (LH) and follicle stimulating hormone (FSH) in KISS1 knockout gilts treated with neurokinin B, kisspeptin, and GnRH hormone analogs [abstract]. Society for the Study of Reproduction Annual Meeting. July 15-19, 2024, Dublin, Ireland. Focus Session 31.
  12. McAnally, B., Nordell, N., Bernhard, C., Lagundzin, D., Sharma, K., Safranski, T., Lucy, M., Desaulniers, A.T. Elucidating the cellular and molecular mechanisms driving testicular dysfunction within in utero growth restricted (IUGR) boars [abstract]. Society for the Study of Reproduction Annual Meeting. July 15-19, 2024, Dublin, Ireland.
  13. Nordell, N.E., Lents, C.A., White, B.R., Desaulniers, A.T. 2024. A porcine model to study lactocrine programming within the testis [abstract]. Swine in Biomedical Research 2024, June 14-18, 2024, Madison, Wisconsin.
  14. Rohrer, G.A., Ostrand, L., Rempel, L.A., Keel-Mercer, B.N., Schmidt, T.B., Mote, B.E. 2024. Genomic regions associated with electronic measures indicative of structural soundness in pigs [abstract]. American Society of Animal Science Annual Meeting. 102(3):24-25. https://doi.org/10.1093/jas/skae234.027.
  15. Ross, C.E., Ahern, D.F., Cederberg, R.A., Choat, F.H., Elsken, D.H., Kurz, S.G., Mills, G.A., Desaulniers, A.T., Lents, C.A., White, B.R. 2024. Preovulatory follicles of GnRH-II receptor knockdown gilts possess fewer hypertrophic theca cells, but similar numbers of hypotrophic granulosa cells compared with littermate controls [abstract]. Society for the Study of Reproduction Annual Meeting. July 15-19, 2024, Dublin, Ireland.
  16. Ross, C.E., Desaulniers, A.T., Lents, C.A., White, B.R. 2025. Gonadotropin-releasing hormone II and its receptor act through theca cells of porcine preovulatory follicles to influence follicular development [abstract]. Midwestern Section of American Society of Animal Science Annual Meeting. 103(1):55-56. https://doi.org/10.1093/jas/skaf102.059.
  17. Ross, C.E., Nordell, N.E., Shelton, J.N., Ahern, D.F., Choat, F.H., Mills, G.A., White, B.R., Desaulniers, A.T. 2024. Elucidating the proteome of seminal plasma-derived extracellular vesicles from GnRH-II knockdown boars with poor semen quality [abstract]. Society for the Study of Reproduction Annual Meeting. July 15-19, 2024, Dublin, Ireland.
  18. Shelton, J., Polivanov, L.J., Nordell, N.E., Qiu, H., Rogan, E.G., Bartelt-Hunt, S.E., Zahid, M., White, B.R., Sillman, S.J., Desaulniers, A.T. 2024. A swine model to investigate the effects of environmentally relevant atrazine exposure on maternal physiology [abstract]. Swine in Biomedical Research 2024, June 14-18, 2024, Madison, Wisconsin.
  19. Shelton, J., Polivanov, L.J., Nordell, N.E., Qiu, H., Rogan, E.G., Bartelt-Hunt, S.E., Zahid, M., White, B.R., Sillman, S.J., Desaulniers, A.T. 2024. Maternal consumption of atrazine impairs offspring birth weight, growth, and organ function in a swine model [abstract]. Swine in Biomedical Research 2024, June 14-18, 2024, Madison, Wisconsin.
  20. White, B.R. 2024. Utilizing GnRH-II receptor knockdown pigs to further explore primary hypogonadism [invited presentation]. Swine in Biomedical Research 2024, June 14-18, 2024, Madison, Wisconsin.
  21. Tyree, M.F., Wolpo, Y.E., Helbus. A.M., Clemens, M.S., Hughes, C.H.K., Stenhouse, C. Characterization of temporal changes in tissue non-specific alkaline phosphatase activity and phosphate concentration in utero-placental tissues and fetal fluids. Society for the Study of Reproduction Annual Meeting. July 2025. Oral Presentation.
  22. Clemens, M.S., Stenhouse, C. Sexual Dimorphism in Porcine Pre-Implantation Embryos. Society for the Study of Reproduction Annual Meeting. July 2025. Poster Presentation.
  23. Mostafa, M.A., Stenhouse, C., Tyree, M.F., Hughes, C.H.K. Orphan Nuclear Receptor SF-1 as a Novel Regulator of Ovarian Reserve Establishment in Cattle. July 2025. Poster Presentation.
  24. DeCastro, L.N., Stenhouse, C., Hughes, C.H.K. Metabolomic signatures of ovarian development in cattle. July 2025. Poster Presentation.
  25. Tyree, M.F., Wolpo, Y.E., Helbus. A.M., Clemens, M.S., Hughes, C.H.K., Stenhouse, C. Characterization of temporal changes in tissue non-specific alkaline phosphatase activity and phosphate concentration in utero-placental tissues and fetal fluids. Huck Life Sciences Symposium 2025. May 23rd Oral Presentation.
  26. Stenhouse, C. Unraveling the mechanisms of utero-placental mineral transport. University of Antwerp, Belgium. March 27th Oral Presentation.
  27. Yankocy, C., Stenhouse, C., Hines, E.A. Assessment of the Variation in Growth Efficiency within Berkshire Pigs. American Society of Animal Science Midwest Section Annual Meeting. March 9-12 2025.
  28. Tyree, M.F., Wolpo, Y.E., Helbus. A.M., Clemens, M.S., Hughes, C.H.K., Stenhouse, C. Characterization of temporal changes in tissue non-specific alkaline phosphatase activity and phosphate concentration in utero-placental tissues and fetal fluids. Gamma Sigma Delta Research Expo. March 27th Poster Presentation.
  29. Stenhouse, C., Alkaline Phosphatase: An Important Regulator of Utero-Placental Phosphate Availability and Conceptus Development. Fertility 2025 – Society for Reproduction and Fertility Emerging Investigator Invited Oral Presentation. January 2025. Liverpool, United Kingdom.
  30. Olivarez, M.O., Lopez, A.N., Stenhouse, C., Moses, R.M., Newton, M.G., Sah, N., Seo, H., Cain, J., Lefevre, C., Ross, A., Wiegert, J., Wu, G., Johnson, G.A., Bazer, F.W. Effects of Dietary Supplementation of Creatine on Placental Development in Mid-and-Late Gestating Gilts. Society for the Study of Reproduction Annual Meeting. July 2024. Poster Presentation. Dublin, Ireland.
  31. Effects of Dietary Supplementation of Monohydrate-Creatine on Fetal Muscle Development in Mid- and Late-Gestating Gilts. Society for the Study of Reproduction Annual Meeting. July 2024. Poster Presentation. Dublin, Ireland.
  32. Stenhouse, C., Halloran, K.M., Moses, R.M., Sah, N., Newton, M.G., Huggins, S., Bertels, J., Suva, L.J., Gaddy, G., Bazer, F.W. Loss of Function Mutations in ALPL Alter Nutrient Abundance in Fetal Plasma, and Allantoic and Amniotic Fluids in Sheep. Mammalian Reproduction Gordon Research Conference. July 2024. Poster Presentation. Barcelona, Spain.
  33. Stenhouse, C., Halloran, K.M., Moses, R.M., Sah, N., Newton, M.G., Huggins, S., Bertels, J., Suva, L.J., Gaddy, G., Bazer, F.W. Loss of Function Mutations in ALPL Alter Nutrient Abundance in Fetal Plasma, and Allantoic and Amniotic Fluids in Sheep. Society for the Study of Reproduction Annual Meeting. July 2024. Poster Presentation. Dublin, Ireland.
Back
Log Out ?

Are you sure you want to log out?

Press No if you want to continue work. Press Yes to logout current user.

Yes
Report a Bug
Report a Bug

Describe your bug clearly, including the steps you used to create it.