Brief Summary of Minutes of Annual Meeting

Brief Summary of Minutes of Annual Meeting

Host: Dr. Alvaro Garcia-Guerra, The Ohio State University

 Monday, May 12^th, 2025

Arrive at the Deer Creek Lodge

Dinner on own

 Tuesday May 13^th, 2024

09:00    Call to order; Alan Ealy, Virginia Tech

            Introductions

            Group business

            Meeting plan

9:15      Short presentations by new members:

                        Maria Gracia Gervasi (Connecticut)

                        Cecilia Rocha (Missouri)

                        Jessica Motto (Maine)

9:30      Alvaro Garcia-Guerra (Ohio)

9:45      Aileen Keating (Iowa)

10:00    Rodolfo Cardoso (Texas)

10:15    Break

10:30    Kamilah Grant and Mark Mirando

            USDA-NIFA Updates

11:00    Jen Wood (Nebraska)  

11:15    Angela Gonella (Florida)

11:30    Julio Giordono (Cornell) 

11:45    Milo Wiltbank (Wisconsin)

12:00    Lunch

1:00      Amanda Patterson (Missouri)

1:15      Shelly Rhoads (Virginia)

1:30      Dan Mathews (Tennessee)

1:45     Camilla Hughes (Pennsylvania)

2:00     Leave for Tour of ST Genetics

            11000 Huntington Rd

            South Charleston, OH 

Group Dinner-to be determined

Wednesday May 14^th, 2025

9:00      Cecily Bishop (Oregon)

9:15     Kosta Siminitras (Louisiana)

9:30      Alan Ealy (Virginia) 

9:45     Graduate Reproductive Biology Course Update

                        Alan Ealy and others 

10:00    New Collaborative Teaching Opportunites

                        Dave Townson and Alan Ealy 

10:30    Break out groups to discuss collaborative research and teaching opportunities

11:15    Reports of collaborative efforts that were discussed

12:00    Adjourn

Accomplishments

Objective 1: Identify Mechanisms that Regulate Ovarian Function and Oocyte Quality during the Estrous Cycle

Determined the effect of Ang II on CCN1 expression, and the activity of gelatinase MMPs (MMP2 and MMP9), in steroidogenic cells obtained from the mid-cycle bovine corpus luteum. Determined the effect of calcium ionophore on CCN1 expression, and the activity of gelatinase MMPs (MMP2 and MMP9) by steroidogenic cells obtained from the mid-cycle bovine corpus luteum. Determined the effects of phytoestrogens in red clover on progesterone (P4) and prostaglandin F2alpha (PGF2α) production in bovine corpora lutea.

Proteomic profiling revealed potential mechanisms regulating prostaglandin F2α responsiveness in bovine corpora lutea

A role of Zinc Insufficiency has been identified in Bovine Granulosa Cell Viability.

A recent set of studies suggests that most of the temporal changes in the CL during early pregnancy that we previously reported are not regulated directly by IFNT, drawing into question identities of other luteal regulators during early pregnancy or the effect of age of CL independent of embryonic signaling.

Recent work described the effect of FSH dose on ovarian response and in vitro embryo production in pregnant cattle with low AMH.

Generated new knowledge related to the impact of preovulatory follicle maturity on oocyte metabolism and competence for embryo development based on metabolome data of maturing oocytes, in vitro embryo development, and RNA-sequencing results of blastocysts. 

Demonstrated that circulating and follicular fluid metabolites are differentially regulated in lactating beef cows of thin, obese, and moderate body composition, and that systemic regulation of reactive oxygen species may be dependent on body condition score.

Explored the extent to which higher estrus associated body temperature advanced preovulatory follicle progression and the metabolome of the preovulatory follicle.  New knowledge was also generated pertaining to the impact of elevated body temperature induced by an acute episode of heat stress after the LH surge impacts preovulatory follicle metabolome. 

Recent work showed that increased DNA damage in oocytes is correlated with diminished autophagy activation.

Heat stress impacts insulin and glucose in dairy cattle experiencing heat stress. This adversely influences preovulatory follicular environment to oocyte quality and competency for embryonic development.  Studies have described the relative contributions of steroid hormones as well as undefined components of the follicular fluid.

An improvement in fertility is observed following displayed estrus in a fixed-time artificial insemination protocol.  Follicular fluid contributes to fertility under these circumstances as blastocyst development was greater when oocytes were exposed to follicular fluid from animals that did display estrus.

Determined that KGN cells, an immortal granulosa cell tumor line, express proteins that are post translationally modified by O-GlcNAcylation and that manipulation of O-GlcNAcylation in vitro impairs KGN proliferation and oxidative phosphorylation.

Demonstrated that manipulation of O-GlcNAcylation in primary cultures of bovine granulosa cells impairs transcript expression of STAR but otherwise has no effect on expression of FSHR and CYP19A1 transcripts, or estradiol production.

Provided preliminary evidence that induction of hypoxia in primary cultures of bovine granulosa cells augments estradiol production; however, manipulation of O-GlcNAcylation does not impact this. This work also identified 354 proteins of the granulosa cell proteome are putatively modified by O-GlcNAcylation, and these proteins encompass a wide range of functional categories including transcription factors, metabolic enzymes, and cytoskeletal proteins.

Identified the signaling pathways and epigenomic factors that regulate RNA binding protein-mRNA interactions during oocyte growth and maturation. We hypothesize that changes in oocyte signaling pathways, i.e., increased ERK1/2, AMPK, and CDK1, that occur after the LH surge, regulate the phosphorylation of BTG4, ELAVL1 ZFP36L2, and MSY2 and subsequently the binding affinity of each RBP to a target 3’UTR.

Determined the effects of luteinizing hormone/protein kinase (PKA) signaling on metabolic pathways driving function of luteal cells and progesterone production.

Explored the signaling and metabolic pathways regulating the function of granulosa cells. We determined the PKA-induced metabolic changes and content of related proteins as well as function of glucose metabolism branches on granulosa cell function.

Examined the roles of metabolites as signaling molecules in the somatic ovarian cells. We found that main kinase triggered by gonadotropins, such as protein kinase A (PKA), enhances histone acetylation and increases chromatin availability in the granulosa cells. 

Objective 2: Determine Factors Associated with Fertilization, Embryo Development, and Conceptus-Endometrial Interactions that Dictate Pregnancy Success

Investigated the role of methyl donors during the periconceptional period and their influence on embryonic development, pregnancy establishment, offspring development, and epigenetic modifications such as DNA methylation. Ongoing research is evaluating how rumen-protected methionine (RPM) supplementation during the periconceptional period (7 days before to 7 days after timed artificial insemination) affects postnatal development of offspring. Additional studies are exploring the impact of RPM supplementation in oocyte donor cows on the composition of follicular fluid and its potential to enhance in vitro bovine embryo production.

Described impacts of environmental exposures on the function of the ovary. Protein profiling of those changes within the ovary are now being associated with loss of ovarian function, changes to hormone production, and impairments to the female gamete.

Explored the functional role of endometrial prostaglandin F₂α in luteal regression after conceptus demise in cattle.

Uncovered the “uterine secretory cycle” as a physiological determinant of preimplantation success. Through temporal transcriptomic profiling of endometrial secretions across the estrous cycle, the work established a foundational framework which governs the cyclical preparedness of the uterus for sperm transit and early embryo development. This highlights how hormone-driven endometrial outputs align precisely with fertilization and preimplantation requirements.

It was revealed that there is a limited responsiveness of the endometrium to embryos during the cycle. The uterine environment is pre-programmed rather than reactively modulated in response to embryos, refining the understanding of when conceptus-endometrial interactions are most critical and sensitive for successful implantation.

Linked uterine luminal conditions to metabolic support of embryo elongation. The identification of a quasi-anoxic uterine environment that favors anaerobic glycolysis provides mechanistic insight into how the uterus metabolically supports rapid conceptus expansion, a critical feature for implantation in Ruminantia.

Completed oral dosing pilot study to determine if gestating beef cows consume enough vitamin A and D precursors via free choice mineral supplement to raise serum levels of vitamin A and D above threshold previously associated with achievement of pregnancy after first artificial insemination cycle post-calving. 

Explored how the interleukin-6 (IL6) cytokine family embryonic disk development and conceptus elongation. Recent discoveries include that supplementing IL6 improves bovine conceptus elongation, that IL6 controls epiblast development, and that LIF preferentially controls hypoblast development.

Antioxidant supplementation can improve in vitro bovine embryo production.

Identified a conditioned media with the capacity to increase day 7 IVP embryo development by 60%. An increase in embryo quality was also observed.

Described endometrial biological responses specific to IVP cattle embryos and IVP cattle embryo specific molecules that elicit these responses.

Expanded vitamin A and D analyses to begin to evaluate effects of vitamin E alone or in combination with A and D on breeding success following first cycle artificial insemination. Future studies will evaluate A, D and E impacts on embryo development and chromosomal integrity. 

Characterized adenomyosis (ectopic endometrial glands and stroma in the myometrium) in postpartum dairy cows and is investigating its effect on fertility. An association between postpartum metritis and the severity of adenomyosis was uncovered, including increased fibrosis, which may impact the receptivity of the uterus to an embryo.

Investigated the impact of metritis/endometritis on postpartum endometrial epithelial regeneration and the effects on fertility. A putative endometrial epithelial stem/progenitor cell population has been identified, and this stem cell is being expanded in diseased endometrium in an attempt to repair the epithelial barrier.

Investigated the feasibility of using endometrial cytology 14 days after artificial insemination without harming the pregnancy outcome. Collecting cytobrush samples on day 14 does not harm the outcome of the pregnancy, and harvested cells are luminal epithelial and glandular epithelial in nature. 

Determined post-transcriptional mechanisms that regulate steroidogenesis by ovarian somatic cells. It is our hypothesis that regulation of steroidogenesis in ovarian granulosa and theca cells during the estrous cycle as well as the change in steroidogenesis during transition of granulosa and theca cells to luteal cells is dependent on changes in RNA methylation and the translation of the proteins that liberate and convert cholesterol to progesterone, androgen, and/or estrogen.

Objective 3: Develop and Evaluate Novel Reproductive Management Strategies and Technologies to Improve Reproductive Performance of Ruminants

Evaluated a novel recombinant hormone protocol for embryo production in Holstein heifers using a single injection of long-acting human follicle-stimulating hormone (FSH). Current investigations are assessing the use of low-dose human chorionic gonadotropin (hCG) at the time of progesterone device withdrawal to support follicular development.

Optimization of synchronization protocols were pursued in an attempt to maximize fertility and reproductive efficiency.

Determined the effect of a targeted reproductive management programs based on automated detection of estrus during the voluntary waiting period on reproductive performance of lactating dairy cows. 

Continued to experiment with estrous synchronization protocols to define how many times progesterone devices can be reused without harming the fertility of the cow.

Provided evidence that combining reproductive outcomes predictors and automated estrus alerts recorded during the voluntary waiting period identified subgroups of cows with different reproductive performance potential.

Demonstrated that delayed induction of ovulation affects the ovarian function of lactating dairy cows synchronized with the Double-Ovsynch protocol and increases insemination after detected estrus.

Developed novel reproductive management programs aimed at increasing fertility of first service through promotion of expression of estrus at the end of synchronization of ovulation protocols.

Identified associations between milk production potential and the profitability of primiparous Holstein cows submitted to insemination with programs that prioritized insemination at detected estrus or timed artificial insemination

Defined a lateral flow-based electronic system for determination of the pregnancy and ovarian status of cattle.

Validated antibody detecting von Willebrand Factor to detect vascularization in sheep ovaries as part of ongoing efforts to study a sheep model of ovarian vitrification and re-transplantation in collaboration with 21^st Century Medicine and Expanse Bio, LLC.

Completed fertility analyses of an additional cohort of ewes in this ovarian vitrification model including performing in vitro fertilization of recovered oocytes from vitrified, warmed and re-transplanted ovaries.

Identified novel candidate genes and proteins that may regulate luteal responsiveness to PGF2A

Evaluated regulation of luteal gene expression by the maternal recognition of pregnancy signal, IFNT, and determined that the luteal gene expression changes that occur in early pregnancy are mostly not regulated by IFNT

Peer-reviewed journal articles (2024

Arneson AG, Stewart JW, Byrd MH, Perry GA, Rhoads ML. 2024. Plasma GABA concentrations in lactating Holstein cows during thermoneutral and heat stress conditions and their relationships with circulating glucose, insulin, and progesterone levels. Vet. Sci. 11:137. doi: 10.3390/vetsci11030137. 

Azari-Dolatabad N, Stokes AE, Hessock EA, Edwards JL, Payton RR, Clark HM, Schrick FN, Moorey SE. 2024. Proestrus, follicle dynamics, and hormone profiles in Jersey cows during synchronization and re-synchronization after preovulatory follicle aspiration. Journal of Animal Science and Research. 8(1). doi:10.16966/2576-6457.167.

Brochado C, Duran B J, Kieffer JD, Pinczak A, Menchaca A, Garcia-Guerra A. 2024. Reduced embryo yield obtained from superstimulated ewes with low ovarian follicular reserve is improved by lengthening the FSH treatment. Theriogenology, 218: 79-88. DOI: 10.1016/j.theriogenology.2024.01.024.

Cavalcante de Souza, D, Gonella-Diaza A, Tonizza de Carvalho NA, Elliff FM, Soares de Carvalho JG, Mendes Vieira L, Bonfim Neto AP, de Carvalho Papa P, Ghuman SS, Madureira EH, Pugliesi G, Binelli M, Baruselli PS 2024. Supplementation with Long-Acting Injectable Progesterone Three Days after TAI Impaired Luteal Function in Buffaloes. Tropical Animal Health and Production 56: article number 76. DOI: 10.1007/s11250-024-03913-3.

Chang WJ, Baker MS, Laritsky E, Gunasekara CJ, Maduranga U, Galliou J, McFadden JW, Waltemyer JR, Berggren-Thomas B, Tate BN, Zhang HH, Rosen BD, Van Tassell CP, Liu GE, Coarfa C, Ren YA, Waterland RA 2024. Systemic interindividual epigenetic variants in cattle share major hallmarks with those in humans. uly 15th. Genome Biology. https://genomebiology. biomedcentral.com/articles/10.1186/s13059-024-03307-6

Clark HM, Stokes AE, Edwards JL, Payton RR, Schrick FN, Campagna SR, Sarumi Q, Hessock EA, Roberts SR, Azaridolatabad N, Moorey SE. 2024. Impact of preovulatory follicle maturity on oocyte metabolism and embryo development. PNAS Nexus. 3(5):pgae181. doi:10.1093/pnasnexus/pgae181.

Ermisch AF and Wood JR. 2024. Regulation of Oocyte mRNA Metabolism: A Key Determinant of Oocyte Developmental Competence. Adv Anat Embryol Cell Biol, 238: 23-46, PMID: 39030353.

Gonella-Diaza A, Sponchiado M, Rodrigues França M, Liu L, Pugliesi G, Guimarães Lo Turco E, Peñagaricano F, Binelli M. 2024. The metabolomic composition of the oviductal fluid is controlled by the periovulatory hormonal context in Bos indicus cows. Biology of Reproduction 111(6): 1188-1201. DOI: 10.1093/biolre/ioae153.

González-Alvarez ME, Antwi-Boasiako C, Keating AF. 2024. Effects of per- and polyfluoroalkylated substances on female reproduction. Toxics. 12(7):455. doi: 10.3390/toxics12070455.

González-Alvarez ME, Inyang I, Keating AF. 2024. Exposure to 7,12-dimethylbenz[a]anthracene impacts ovarian DNA damage sensing and repair proteins differently in lean and obese female mice and weight loss may mitigate obesity-induced ovarian dysfunction. Toxicology and Applied Pharmacology. 486:116930. doi: 10.1016/j.taap.2024.116930

Hayden C B, Motta JCL, Sala RV, Bello N, Coutinho da Silva M, García-Guerra A. 2024. Efficacy of methods to synchronize follicular wave emergence in pregnant heifers. JDS Communications, JDSC.2024-0629.R1, In press. DOI: 10.3168/jdsc.2024-0629.

Hill KS, Edwards JL, Payton RR, Schrick FN, Campagna SR, Hessock EA, Moorey SE. 2024. Relationships of circulating and preovulatory follicular fluid hydrogen peroxide level with body condition score and metabolome profiles of lactating beef cows. Agriculture. 14(8):1406. doi: 10.3390/agriculture14081406.

Inyang I, White HE, Timme K, Keating AF. 2024. Biological sex differences in hepatic response to in utero dimethylbenz[a]anthracene exposure. Reproductive Toxicology. 124:108553. doi: 10.1016/j.reprotox.2024.108553. PMID: 38307155.

Joyce K, Gad A, Menjivar NG, Gebremedhn S, Heredia D, Dubeux G, Lopez-Duarte MC, Bittar J, Gonella-Diaza A, Tesfaye D. 2024. Seasonal environmental fluctuations alter the transcriptome dynamics of oocytes and granulosa cells in beef cows. Journal of Ovarian Research 17(1): article number 201. DOI: 10.1186/s13048-024-01530-0.

Keane JA, Ealy AD. 2024. An Overview of Reactive Oxygen Species Damage Occurring during In Vitro Bovine Oocyte and Embryo Development and the Efficacy of Antioxidant Use to Limit These Adverse Effects. Animals (Basel). Jan 21;14(2):330. doi: 10.3390/ani14020330.

Keating AF, Ross JW, Baumgard LH. 2024. Systemic and Ovarian Impacts of Heat Stress in the Porcine Model. Reproduction. Nov 11;168(6):e240217. doi: 10.1530/REP-24-0217.

Maucieri AM, Townson DH 2024. Influence of O-GlcNAcylation on KGN cell function. Endocrine and Metabolic Science 16:100192.

Mirzaei A, Londoño-Mendez MC, Lasso-Ramirez S, Adams PE, Seekford ZK, Bromfield JJ, Heredia D, Di Lorenzo N, Chebel RC, Gonella-Diaza AM. 2024. Embryo production by Holstein heifers superovulated with a recombinant long-acting follicle stimulating hormone analog. Journal of Animal Sciences 102: skae326. DOI: 10.1093/jas/skae326.

Mills MD, Pollock AB, Batey IE, O’Neil MA, Schrick FN, Payton RR, Moorey SE, Fioravanti P, Hipsher W, Zoca SM, Edwards JL. 2024. Magnitude and persistence of higher estrus associated temperatures in beef heifers and suckled cows. Journal of Animal Science. 102. Skae079. doi: 10.1093/jas/skae079.

Novbatova G., Timme K, Keating AF. 2024. High fat diet-induced obesity and gestational DMBA exposure alter folliculogenesis and the proteome of the maternal ovary. Biology of Reproduction. 111(2):496-511. doi: 10.1093/biolre/ioae070.

Laplacette AL, Rial C, Sitko EM, Perez MM, Tompkins S, Stangaferro ML, Thomas MJ, Giordano JO. 2024. Delaying induction of ovulation and timed artificial insemination in a Double-Ovsynch protocol increased expression of estrus and altered first-service reproductive outcomes of lactating dairy cows. J. Dairy Sci. 2024 Oct 14:S0022-0302(24)01216-5. https://doi:10.3168/jds.2024-24994.

Oliver MA, Speckhart SL, Edwards JL, Rhoads ML, Ealy AD. 2024. Human recombinant interleukin-6 improves the morphological quality of cryopreserved in vitro produced bovine blastocysts. Theriogenology. 226:173-180. doi: 10.1016/j.theriogenology.2024.06.014. 

Ott TL, Tibary A, Waqas M, Geisert R, Giordano JO. 2024. Pregnancy establishment and diagnosis in livestock. Annual Review of Animal Biosciences, 13. https://doi.org/10.1146/annurev-animal-021022-032214

Plewes MR, Talbott HA, Schott MB, Wood JR, Cupp AS, Davis JS. 2024. Unraveling the role of lipid droplets and perilipin 2 in bovine luteal cells. FASEB J 38:e23710, PMID: 38822676.

Przygrokzka E, Binderwala F, Powers R, McFee RM, Cupp AS, Wood JR, Davis JS. 2024. Metabolic control of luteinizing hormone-responsive ovarian steroidogenesis. J. Biol Chem 301:108042 PMID: 39615688, PMCID: PMC11732475.

Rial C, Giordano JO. 2024. Combining reproductive outcomes predictors and automated estrus alerts recorded during the voluntary waiting period identified subgroups of cows with different reproductive performance potential. J. Dairy Sci. 107:7299-7316. https://10.3168/jds.2023-24309

Roach CM, Mayorga EJ, Baumgard LH, Ross JW, Keating AF. 2024. Heat stress alters the ovarian proteome in pre-pubertal gilts. Journal of Animal Science. doi: 10.1093/jas/skae053. PMID: 38605681; PMCID: PMC11025630.

Roach CM, Mayorga EJ, Baumgard LH, Ross JW, Keating AF. 2024. Phenotypic, endocrinological, and metabolic effects of zearalenone exposure and additive effect of heat stress in prepubertal female pigs. Journal of Thermal Biology. 119:103742. doi: 10.1016/j.jtherbio.2023.103742. 

Roach CM, Mayorga EJ, Baumgard LH, Ross JW, Keating AF. 2024. Zearalenone exposure differentially affects the ovarian proteome in prepubertal gilts during thermal neutral and heat stress conditions. Journal of Animal Science. 102:skae115. doi: 10.1093/jas/skae115.

Rishi JK, Timme K, White HE, Kerns KC, Keating AF. 2024. Altered histone abundance as a mode of ovotoxicity during 7,12-dimethylbenz[a]anthracene exposure with additive influence of obesity. Biology of Reproduction. 110(2):419-429. PMID: 37856498. DOI: 10.1093/biolre/ioad140.

Rishi JK, Timme K, White HE, Kerns KC, Keating AF. 2024. Trajectory of primordial follicle depletion is accelerated in obese mice in response to 7,12-dimethylbenz[a]anthracene exposure. Biology of Reproduction. 111(2):483-495. doi: 10.1093/biolre/ioae059.

Sahoo PK, Krishnamoorthy C, Wood JR, Hanson C, Anderson-Berry A, Mott JL, Natarajan SK. 2024. Palmitate induces integrated stress response and lipoapoptosis in trophoblasts. Cell Death and Disease, 15:31, PMID: 38212315 

Schalich KM, Koganti PP, Castillo JM, Reiff OM, Cheong SH and Selvaraj V. 2024. The uterine secretory cycle: recurring physiology of endometrial outputs that setup the uterine luminal microenvironment. Physiological Genomics. 56(1):74-97. https://doi.org/10.1152/physiolgenomics.00035.2023

Senn, L. K., K. D. Peterson, J. L. Edwards, R. R. Payton, and D. J. Mathew. Oviduct and endometrial epithelium improve IVP bovine embryo developmental kinetics. Reproduction. 2024. 167 e240008.  doi:10.1530/REP-24-0008.

Speckhart SL, Oliver MA, Keane JA, Dias NW, Mercadante VRG, Biase FH, Ealy AD. 2024. Interleukin-6 supplementation improves bovine conceptus elongation and transcriptomic indicators of developmental competence. Biol Reprod. Jul 12;111(1):43-53. doi: 10.1093/biolre/ioae045.

Studer JM, Kiefer ZE, Koester LR, Johnson EM, Schmitz-Esser S, Farkas A, Galina Pantoja L, Vonnahme KA, Greiner LL, Keating AF, Baumgard LH, Ross JW. 2024. Evaluation of circulating immune cells, analytes, and inflammatory markers in sows affected with postpartum dysgalactia syndrome. Journal of Animal Science. doi: 10.1093/jas/skae270. 

Sun F Ali NN, Londoño-Vásquez D, Simintiras CA, Qiao H, Ortega MS, Agca Y, Takahashi M, Rivera RM, Kelleher AM, Sutovsky P, Patterson AL, Balboula AZ. Increased DNA damage in full-grown oocytes is correlated with diminished autophagy activation. Nature Communications. 2024. 19: 9463.

Timme K, González-Alvarez, ME, Keating AF. Pre-pubertal obesity compromises ovarian oxidative stress, DNA repair and chemical biotransformation. Toxicology and Applied Pharmacology. 2024. 489:116981. doi: 10.1016/j.taap.2024.116981.

Book Chapters (2024)

Bishop CV. Corpus Luteum (Encyclopedia of Reproduction, 3^rd Edition). 2024. Elsevier.

González-Alvarez ME, Kiefer ZE, Studer JM, Ross JW, Keating AF. 2024. Understanding ovarian function in pigs. Advances in pig breeding and reproduction. In Press. BDS Publishing. J.W. Ross (Ed.).

Conference Proceedings (2024)

Garcia Guerra A, Motta JCL, Hayden CB, Sala RV, Pereira DC, Absalon-Medina A, Moreno JF, Ross. J. Manejo reproductivo de la donante para la producción exitosa de embriones in vitro. (Translation: Reproductive management of the donor for successful in vitro embryo production). Proceedings of the 9° Simposio Internacional Avances en Reproducción Bovina (9^th International Symposium Advances in Bovine Reproduction), August 1-2^nd, 2024, Guadalajara, Jalisco, México, Pages 1-17.

Garcia Guerra A, Sala RV, Motta JCL, Wiltbank MC. Manejo de receptoras de embriones para una reproduccion exitosa: fertilidad y eficiencia. (Translation: Management of Embryo Recipients for Successful Reproduction: Fertility and Efficiency). Proceedings of the 9° Simposio Internacional Avances en Reproducción Bovina (9^th International Symposium Advances in Bovine Reproduction), August 1-2^nd, 2024, Guadalajara, Jalisco, México, Pages 119-137.

Garcia Guerra A, Rojas Cañadas E, Crist AE, Folchini NP, Mussard ML. Avances en programas de sincronizacion para IATF en ganado de carne: estrategias de presincronización. (Translation: Advances in synchronization programs for fixed time AI in beef cattle: presynchronization strategies). Proceedings of the XXVI Congreso Internacional Anembe de Medicina Bovina (XXVI International Congress of Bovine Medicine Anembe) 2024, April 24 - 26, Córdoba, Spain, Pages 200-212.

Garcia Guerra A, Duran B, Motta JCL, Sala RV, Wiltbank MC. Perdidas gestacionales: bases fisiologicas y estrategias para reducirlas. (Translation: Pregnancy loss: physiological bases and mitigating strategies). Proceedings of the XXVI Congreso Internacional Anembe de Medicina Bovina (XXVI International Congress of Bovine Medicine Anembe) 2024, April 24 - 26, Córdoba, Spain, Pages 184-199.

Ting A, Weegman B, Bishop CV, Wowk B, Chow L, Pagotan R, Stricklin OE, Fox AK, Harrington SS, Weiss T, Estill C, Fahy G, Zelinski M. (2024). Functional preservation after ovarian tissue and whole ovary vitrification and auto-transplantation in large animal models (vol. 117, pp. 105023). Cryobiology https://www.sciencedirect.com/science/article/pii/S0011224024001780.

Abstracts (2024)

Adeyanju O, González-Alvarez ME, Antwi-Boasiako, C, Polivanov LJ, Shelton J, Nordell NE, Sillman SJ, Laws MJ, Raetzman L, Flaws JA, Desaulniers AT, Keating AF. 2024. Female offspring ovarian chemical metabolism protein abundance is altered by in utero atrazine exposure. Society for Study of Reproduction annual meeting.

Alward KJ, Oliver MA, Johnson SE, Ealy AD. In Vitro Embryo Production Can Be Achieved After Delayed Harvest of Bovine Oocytes from Abattoir-derived Ovaries. 2024. Society for the Study of Reproduction Annual Meeting, Dublin Ireland.

Antwi-Boasiako C, González-Alvarez ME, Romoser MR, Ross JW, Baumgard LH, Keating AF. 2024.  The effects of heat stress on the abundance of proteins involved in chemical biotransformation in the corpora lutea of post-pubertal gilts. Society for Study of Reproduction annual meeting.

Baber T, Newman S, Joyce K, Rhoads ML. 2024. Relationships between circulating glucose concentrations and productivity in heat-stressed dairy cattle. J. Dairy Sci. 107(suppl 1): 320.

Bishop CV, RancHER. 2024. Strategies for Preparing Low Parity Heifers and Cows for Their Next Breeding Season, Online. National, Invited..

Bowne S, Ting A, Bishop CV. 2024. Northwest Reproductive Sciences Symposium, "Use of Anti-Mullerian Hormone to Determine Oocyte Aspiration Success in Synchronized Sheep.," Bend, OR. Regional, Accepted.

Branco L. Henning J, Rocha, CC. 2024. Rescuing the weight loss caused by toxic calves in calves. CAFNR Research Symposium, Columbia MO.

Carothers A , Diaz FJ. 2024. Role of Zinc Insufficiency in Bovine Granulosa Cell Viability. 57^th annual meeting of the Society for the Study of Reproduction, July 15-19, 2024, Dublin, Ireland (poster presentation).

Crist AE, Motta JCL, Hayden CB, Rykaczewski C, Mussard MM, Garcia-Guerra A. 2024. Characterization of anovular phenotypes in postpartum beef cows. Reproduction Fertility and Development 36(2):230-231. DOI: 10.1071/RDv36n2Ab154

Davis JS, Przygrodzka E, Plewes MR. 2024. Control of Luteal Function by Luteinizing Hormone: Insights into multi-organelle control of steroidogenesis. The 6th International Conference on Uterine Disorders and Corpus Luteum Function. September 9–12, 2024, Wroclaw, Poland.

Ermisch AF, Shao J, Beede K, Schmaltz R, Ramer-Tait A, Wood JR. 2024. Luteinizing hormone stimulation of germ-free and conventionalized female mice reveals microbiome-dependent effect on ovarian steroidogenesis and ovulation. 57^th Annual Meeting of the Society for the Study of Reproduction, Dublin, Ireland. 

Ermisch AF and Wood JR. 2024. Comparison of N⁶-methyladenosine modification of mRNA during the bovine and murine oocyte to embryo transition. 50^th Annual Meeting of the International Embryo Transfer Society, Denver, CO.  

Folchini NP, Crist AE, Motta JCL, Wellert S, Rykaczewski C, Saad M, Carranza Martin AC, Garcia-Guerra A. 2024. Expression of estrus and fertility of different presynchronization strategies for fixed-time artificial insemination in suckled beef cows. Reproduction Fertility and Development 36(2):244-245. DOI: 10.1071/RDv36n2Ab181

Fox AK, Hammerich MS, Bishop CV. 2024. Northwest Reproductive Sciences Symposium, Vitamin A and D Pre-exposure to Prime Reproductive Success in Breeding Beef Cows. Bend, OR. Regional, Accepted.  

Garcia-Guerra A, Motta JCL, Crist AE, Folchini NP, Rykaczewski C, Wellert S. 2024. Optimization of the time of insemination and presynchronization treatment in beef heifers submitted to fixed-time artificial insemination. Reproduction Fertility and Development 36(2):243. DOI: 10.1071/RDv36n2Ab178

Giordano JO. 2024. Ovarian physiology of previously inseminated cows: Considerations for rebreeding. J. Dairy Sci. 107 (E-Suppl. 1). https://www.adsa.org/Meetings/Past-Meetings/2024

González-Alvarez ME, Timme K, Keating AF. 2024. Effect of DMBA exposure on ovarian chemical metabolism, DNA damage sensing and repair, and oxidative stress proteins in prepubertal mice. Society for Study of Reproduction annual meeting.

Haffner C, Rishi JK, Keating AF. 2024. Altered abundance of proteins involved in chemical biotransformation in inguinal adipose tissue in lean and obese female mice during exposure to dimethylbenz[a]anthracene. Society for Study of Reproduction annual meeting.

Henning J, Branco L, Rocha CC. 2024. Using anogenital distance to predict probability of pregnancy to artificial insemination in beef heifers. CAFNR Research Symposium, Columbia MO.

Heredia DC, Tarnonsky F, Lopez-Duarte MC, Venturini M, Ojeda OA., Luchini D., Hansen PJ, Pringle D, DiLorenzo N, Gonella-Diaza AM. 2024. Impact of supplementing rumen-protected methionine during the periconceptional period in the post-weaning period in female calves, Journal of Animal Science, 102 (S1): 69–70, DOI: 10.1093/jas/skae019.081

Hughes CHK, Hellmers AC, daSilva MI, Ott TL, Pate JL. 2024. IFNT-dependent and IFNT-independent changes in the CL of early pregnancy. 57^th annual meeting of the Society for the Study of Reproduction, July 15-19, 2024, Dublin, Ireland (poster presentation).

Hughes J, Hughes CHK. 2024. Proteomic profiling reveals potential mechanisms regulating prostaglandin F2A responsiveness in bovine corpora lutea. Annual meeting of the American Dairy Science Association, June 16-19, 2024, West Palm Beach, FL (poster presentation).

Keane JA, Oliver MA, Pollock AB, Alward KJ, McGehee JF, Kesler ML, Snider AP, Ealy AD.

Impacts of OmniGen-AF^® Feed Supplementation on Ovum Pick-Up Collections in Beef Cattle. Society for the Study of Reproduction Annual Meeting, Dublin Ireland.

Laplacette AL,  Stangaferro ML, Thomas MJ, Giordano JO. 2024. Reproductive performance of lactating dairy cows managed with a targeted reproductive program aimed at increasing AI in estrus after synchronization of ovulation. J. Dairy Sci. 107 (E-Suppl. 1). https://www.adsa.org/Meetings/Past-Meetings/2024

Laplacette AL,  Stangaferro ML, Thomas MJ, Giordano JO. 2024. A reproductive management program aimed at maximizing insemination of cows at detected estrus after synchronization of ovulation improved first service outcomes. J. Dairy Sci. 107 (E-Suppl. 1). https://www.adsa.org/Meetings/Past-Meetings/2024

Laws MJ, Kramer S, Weis K, Polivanov LJ, Shelton J, Nordell NE, White BR, Sillman SJ, Raetzman L, Keating AF, Flaws JA, Desaulniers AT. 2024. Environmentally Relevant Maternal Atrazine Exposure Leads to Aberrant Endocrine Effects in Piglets. Society for Study of Reproduction annual meeting.

Mathew DJ, Peterson KD, Freeman TF, Oliver MA, Kakhniashvili D, Johnson DL, Payton RR, Prado TM. Strickland LG, Edwards JL, Beever JE. 2024. Processes and pathways associated with in vivo derived and in vitro produced male and female bovine conceptus proteins. 57^th Annual Society for the Study of Reproduction (SSR), Dublin, Ireland. Abstract and poster presentation.

Moorey SE. Role of the preovulatory follicle in oocyte metabolic and developmental competence for pregnancy. 2024. American Society of Animal Science / Canadian Society of Animal Science / Western Section American Society of Animal Science Joint Annual Meeting; Biennial Reproduction Symposium. Calgary, Alberta, Canada. Invited abstract and oral presentation.

Motta JCL, Carranza-Martin AC, Rykaczewski C, Folchini NP, Saad M, Hayden C, Sala RV, Bond R, Pereira DC, Ross PJ, Garcia-Guerra A. 2024. Anti-Müllerian hormone: how early can it be used as a biomarker for future in vitro embryo production in Bos taurus cattle? Reproduction Fertility and Development 36(2):250-251. DOI: 10.1071/RDv36n2Ab191

Nikolaou TA, Rial C, Hussain I, Giordano JO, Erickson D. 2024. ReproPhone: A lateral flow based portable platform for the determination of cattle reproductive status. Biomedical Engineering Society. 2024. Baltimore, MD., October 2024.

Oliver MA, Johnson SE, Ealy AD. 2024. Supplementation of Leukemia Inhibitory Factor or Interleukin 6 Modifies the Cellular Lineage Composition within the Embryonic Disc of Extended Cultured Bovine Blastocysts. Society for the Study of Reproduction Annual Meeting, Dublin Ireland. 

Pollock AB, Oliver MA, Johnson SE, Ealy AD. 2024. Chemical Inhibition of IL6ST Compromises the In Vitro Production of Bovine Embryos. Society for the Study of Reproduction Annual Meeting, Dublin Ireland. 

Reamsnyder T, Sala RV, Pinete-Gonzalez A, Maldonado E, Robles-Morado I, Martinez LC, Absalon-Medina VA, Fricke VC, Juarez-Dorantes RI, Ross PJ, Moreno JF, Motta JCL, Garcia-Guerra A. 2024. “Effect of length of progesterone device administration during a modified CO-Synch for fixed-time embryo transfer in heifers” Reproduction Fertility and Development 36(2):209-210. DOI: 10.1071/RDv36n2Ab114

Rishi JK, Montes C, Walley JW, Keating AF. 2024. Obesity alters sumoylation-dependent proteomic response in ovaries of mice exposed to 7,12-dimethylbenz[a]anthracene. Society for Study of Reproduction annual meeting.

Roa-Vidal P, Markway M, Moraes JGN, Lucy MC, Patterson AL. 2024. Uncovering the spatial gene expression of the bovine uterus: a novel study model for adenomyosis. Poster presented at the Gilbert S. Greenwald Symposium Annual Meeting, Kansas City Kansas.

Roa-Vidal P, Medeiros de Almedia J, Wang Z, Patterson AL. 2024. Establishing a bovine in vitro model to investigate the mechanisms of adenomyosis development. Poster presented at the Translational Bioscience PhD Program Symposium, MU School of Medicine, Columbia MO.

Rose PA, Bidne KL, McCain AR, Shankar K, Wood JR. 2024. Maternal obesity is associated with global reductions in gene expression including mRNAs that contribute to cell migration and glucocorticoid metabolism in the mid-gestation mouse placenta. 57^th Annual Meeting of the Society for the Study of Reproduction, Dublin, Ireland.

Saad M, Duran BJ, Rykaczewski C, Kieffer JD, Menchaca A, Garcia-Guerra A. 2024. Effect of P.G. 600 dose on expression of estrus, fertility, and prolificacy in ewes synchronized out-of-season using a short-term progesterone-based protocol. Reproduction Fertility and Development 36(2):245. DOI: 10.1071/RDv36n2Ab182

Sala RV, Motta JCL, Absalon-Medina VA, Fricke VC, Crist AE, Reamsnyder T, Ross PJ, Moreno JF, Garcia-Guerra A. 2024. Assessment of porcine follicle-stimulating hormone delivery mode before ovum pickup and in vitro embryo production in pregnant heifers. Reproduction Fertility and Development 36(2):250. DOI: 10.1071/RDv36n2Ab190

Sellmer Ramos I, Caldeira MO, Patterson AL, Lucy MC. 2024. Spatial transcriptomics of the neonatal bovine uterus reveals potential markers of epithelial differentiation driving endometrial adenogenesis. Poster presented at the Society for the Study of Reproduction Annual Meeting, Dublin Ireland.

Sitko EM, Giordano JO. 2024. Effect of prioritizing AI in estrus or timed AI and milk yield on cash flow of primiparous cows. J. Dairy Sci. 107 (E-Suppl. 1). https://www.adsa.org/Meetings/Past-Meetings/2024

Sitko EM, Laplacette AL, Duhatschek D, Rial C, Perez MM, Tompkins S, Kerwin AL, Domingues RR, Wiltbank MC, Giordano JO. 2024. The ovarian function and endocrine phenotypes of lactating dairy cows were associated with genomic merit for fertility. J. Dairy Sci. 107 (E-Suppl. 1). https://www.adsa.org/Meetings/Past-Meetings/2024

Timme K, González-Alvarez ME, Keating AF. 2024. Both obesity and DMBA exposure alter ovarian follicle number, ZP3 and PPP2CA in prepubertal females. Society for Study of Reproduction annual meeting. 

Venturini M, Heredia DC, Lopez MC, Joice K, Ruiz M, Dubeux JCB,  Dilorenzo N, Gonella-Diaza AM. 2024. Plasma metabolomics and feed behavior patterns in feed efficient or inefficient beef bulls. Journal of Animal Science, 102 (S1): 1–2, DOI: 10.1093/jas/skae019.001

Weiss T, Ting A, Bishop CV. 2024. Northwest Reproductive Sciences Symposium, Optimization of Immunohistochemistry Techniques for the Use of Polyclonal Rabbit Anti-Human von Willebrand Factor to Detect Ovarian Vascularization in Ovine Cryopreserved Ovaries, Bend, OR. Regional, Accepted.

Wiltbank MC, Andrade JP, Gomez-Leon V, Garcia-Guerra A, Monteiro PLJ, Domingues RR, Fricke PM, Sartori R. 2024. The dichotomy between pregnancy survival and loss: Challenging post-conceived notions. Journal of Dairy Science 107(Supp 1):170.