Brief Summary of Minutes of Annual Meeting

Accomplishments

OBJECTIVE 1: Characterize host mechanisms associated with mastitis susceptibility, and resistance to improve economic outcomes and animal welfare.

University of Minnesota

Members: S. Godden, L. Caixeta, N. Noyes, B. Crooker

Our group addressed objective 1 through two major avenues: 1) the impact of host genotype on immune response in the context of mastitis; and 2) the association between host-associated microbiomes and mastitis risk.

Michigan State University

Member: Pamela Ruegg

In 2023, we completed publications that characterized the role of oxylipids in modulating the immune response during involution.  We also collaborated on several projects characterizing biomarkers in blood and milk associated with inflammation caused by intramammary infection.

University of Vermont Agriculture Station

Members: Feng-Qi Zhao, John Barlow

Contributors: Chrissy Lalonde

Dr. Zhao’s group studied the association between serum prostaglandin PGE2 levels and the Δ9 desaturation index (DI) of fatty acids in plasma and milk triacylglycerides (TAG) during mastitis. In the first 24 hours of intramammary lipopolysaccharide injection, serum PGE2 concentrations are moderately associated with the Δ9 DI of fatty acids in plasma TAG and milk fatty acids of > 16 carbons. This indicates that systemic PGE2 may be implicated in milk lipid changes seen during mastitis.

OBJECTIVE 2:  Characterize agents associated with intramammary infections and assess their impact on milk quality and animal welfare

University of Minnesota

Members: S. Godden, L. Caixeta, N. Noyes, B. Crooker

Our station has been contributing to objective 2 by assessing phenotypic and genotypic characteristics of non-aureus Staphylococci and Mammaliicocci (NASM) isolated from the teat apices of dairy cows. Specifically, we are investigating NASM isolates that exhibit inhibitory activity against S. aureus and S. uberis.

University of Missouri

Members: Pamela Adkins, John Middleton                                                                       

Contributors: Alyssa Novo, Paige Isensee

Our group focused on the evaluation of intramammary infection (IMI) status in primigravid and nulligravid dairy heifers using cisternal and teat end sampling techniques. Overall, we identified the prevalence of IMI was higher in primigravid compared to nulligravid heifers.  Additionally, in both groups, non-aureus staphylococcal species were the most common bacteria identified among all sample types. Our group also continued to work on characterization of antibacterial activity and strain variability among Staphylococcus chromogenes isolates originating from dairy cattle.  Overall, in vitro phenotypic growth inhibition of Staphylococcus aureus isolates by S. chromogenes isolates was rarely identified.

Michigan State University

Member: Pamela Ruegg

MSU researchers completed a clinical trial evaluating etiologies and bacteriological clearance of Gram-positive pathogens that were recovered from milk samples of cows with non-severe clinical mastitis.  Lactococci were the most commonly recovered genus. Spontaneous bacteriological clearance and therapeutic clearance were estimated and minimum inhibitory values for commonly used intramammary antimicrobials were determined for Lactococci, Enterococci, Staphylococci and Streptococci. Additional projects have been focused on determining the impact of intramammary and systemic treatments on the microbiota and abundance of resistant bacteria and resistance genes found in manure of treated cows.

Hill Farm Research Station, LSU AgCenter

Member: William E. Owens

Identify and evaluate bacteria causing bovine mastitis in Louisiana.  Test bacteria for antimicrobial resistance to mastitis therapeutics. Stock mastitis isolates and share with other members for further testing and evaluation. Advise dairy producers and fieldmen on mastitis prevention and control methods.

University of Vermont Agriculture Station

Members: Feng-Qi Zhao, John Barlow

In this year the Barlow lab finalized the development of a long-read first hybrid whole genome sequencing pipeline that assembles Oxford Nanopore long-read and Illumina short-read sequences to generate high-quality “closed” genomes of mastitis isolates. We applied this to sequencing S. aureus isolates from cases of clinical and subclinical mastitis. The Barlow lab completed a longitudinal field study of mastitis epidemiology on 10 organic dairy farms, with a focus on describing the diversity and frequency of Staphylococcus mastitis on these farms.

OBJECTIVE 3: Assess and apply new technologies and preventative strategies that advance mastitis control, milk quality and/or reduce antimicrobial usage

University of Minnesota

Members: S. Godden, L. Caixeta, N. Noyes, B. Crooker

Our group worked on several technologies and preventative strategies to advance mastitis control and reduce antimicrobial usage, including randomized clinical trials of acidogenic boluses and several studies of selective dry cow therapy. Additionally, our group contributed to the advancement of applied next-generation sequencing for mastitis research by more fully characterizing the microbiome of milk obtained from dairy cows, including the contaminant profile.

Rutgers University; New Jersey Agricultural Experiment

Member: Jeffrey Boyd

Contributors: Kylie Ryan Kaler, Taylor Andrews, Javiara Norambuena

Most of this year we have focused our efforts in examining new mechanisms to prevent the growth of Staphylococcus aureus. We discovered that when S. aureus is treated with copper ions, they are imported using a manganese and zinc ion import systems. We also found that we can trick S. aureus into bringing in excess copper by starving the organism for zinc or manganese. Once inside the cell, they poison the pentose phosphate pathway leading to S. aureus death. We also discovered that S. aureus with defective pentose phosphate pathway is defective in pathogenesis.

Michigan State University

Member: Pamela Ruegg

MSU researchers had several projects under this objective.  One project was focused on determining risk factors for methicillin resistance in non-aureus Staph recovered from bulk tank milk.  While the prevalence of MR-NAS in bulk tank milk was low, we did determine that the presence of swine on smaller dairy farms increased the risk. 

Several of our projects were related to quantifying antimicrobial usage on dairy farms and developing mechanisms to impact antimicrobial use (AMU) on dairy farms.  Our team created and validated software that uses farm records to quantify AMU and compare to peer farmers.  We have been working on identifying the economic impact of treatment decisions with the goal of determining how to better motivate dairy farmers to reduce duration of antimicrobial treatments and to adopt selective treatment strategies for dry cow treatments and for treatment of non-severe clinical mastitis.

WORK PLANNED FOR THE COMING YEAR, LISTED BY OBJECTIVE:

OBJECTIVE 1

Cornell University

Members: Quinn Kolar, Matthias Wieland

Collaboration on “Solutions for controlling mastitis and improving milk quality in organic dairy farms: An Integrated Approach” (OREI/USDA) to identify host factors that contribute to mastitis susceptibility on organic dairy farms and how to broaden the uptake of preventative strategies. Collaboration with Colorado.

University of Minnesota

Members: S. Godden, L. Caixeta, N. Noyes, B. Crooker

We plan to tackle this objective through continued analysis of a host-microbiome interaction challenge study in which two groups of cows with different genetic backgrounds were challenged with intramammary E. coli (study 1) and S. uberis (study 2). We obtained longitudinal host transcriptomic and microbiome data from the challenged cows, both before and after the challenge. Results will be compared by genotype to elucidate interactions between genotype, immune response and host microbiomes during mastitis challenge.

Additionally, we will investigate the effect of mucosal immune stimulation on mammary gland immune responses during the dry period and early lactation period in dairy cows. The two objectives of this project are to determine the effect of MucosImmune on (1) the innate immune response in the mammary gland during the period immediately before and after parturition, and (2) the resistance to bacterial infection of the mammary gland. Ongoing experiments will be continue until summer 2025.

South Dakota State University

Member: Turner Swartz

In 2024, we received funding from USDA-NIFA-AFRI to explore the role of systemic inflammation during the peripartum period on mammary gland immune responses during an intramammary challenge in dairy cattle. We are currently conducting the studies outlined in this proposal and will begin reporting results in 2025.                         

University of Vermont Agriculture Station

Members: Feng-Qi Zhao, John Barlow

Zhao’s group will continue to focus on the effects of mastitis on lipid metabolism in mammary epithelial cells.

OBJECTIVE 2

University of Minnesota

Members: S. Godden, L. Caixeta, N. Noyes, B. Crooker

We will continue our work on objective 2 by assessing phenotypic and genotypic characteristics of additional non-aureus Staphlococci and Mammalococci (NASM) isolated from the teat apices of dairy cows; and we will expand this to Bacilli. Our main goal is to understand the mechanisms of inhibitory activity.

University of Missouri

Members: Pamela Adkins, John Middleton

We plan to continue to work on the molecular characterization of Staphylococcus chromogenes isolates using various strain typing techniques. Our goal is to identify a simple and fast method that can distinguish isolate with various phenotypic characteristics.

Michigan State University

Member: Pamela Ruegg

We are performing a field trial on dairy farms to establish spontaneous bacteriological cure and impact on performance and productivity for cows with non—treated clinical and subclinical mastitis

Hill Farm Research Station, LSU AgCenter

Member: William E. Owens

We will continue to identify and evaluate bacteria causing bovine mastitis in Louisiana.  Test bacteria for antimicrobial resistance to mastitis therapeutics. Stock mastitis isolates and share with other members for further testing and evaluation. Advise dairy producers and fieldmen on mastitis prevention and control methods.

Utah State University

Member: David Wilson

Contributors: Gregory Goodell, Tessa Kelly, Rick Dumm, Michaela Bethard, John Wallentine, Jon Schumann, Shane Summers, Justin Jenson, Eneli Jenson, Kyson Jenson, Kortnee Selin                                        

Continue the evaluation of a preservative for milk, colostrum and milk replacer fed to calves.  A calf feeding trial is nearly completed comparing multiple concentrations of the preservative that has been associated with significantly reduced bacteria counts over time in comparison to those in untreated post-pasteurized milk.  Other applications in dairy and other food industries have found these concentrations safe including with no withdrawal time for human consumption.  This is a safety and palatability trial, with preliminary evaluation of pre-weaned calf disease or mortality outcomes.  We will soon begin another study of usage of the preservative.

University of Vermont Agriculture Station

Members: Feng-Qi Zhao, John Barlow

The Barlow lab will complete whole genome sequencing of Staphylococcus aureus isolates to identify potential virulence factors and antimicrobial resistance genes, and explore the phylogeny and ecology of Staphylococcus species and mobile genetic elements among these species. The Barlow lab will continue to explore the epidemiology of Staphylococcus aureus and non-aureus staphylococci in small to medium sized dairy farms.

OBJECTIVE 3

Cornell University

Members: Quinn Kolar, Matthias Wieland

Collaboration on “Solutions for controlling mastitis and improving milk quality in organic dairy farms: An Integrated Approach” (OREI/USDA) to identify host factors that contribute to mastitis susceptibility on organic dairy farms and how to broaden the uptake of preventative strategies. Collaboration with Colorado.

University of Minnesota

Members: S. Godden, L. Caixeta, N. Noyes, B. Crooker

Our plans for objective 3 in the next year are as follows:

2025. Knauer L.S. Caixeta, S. Godden, D. Nydam. Randomized clinical trial evaluating the effect of administering Bovikalc® Dry the day before dry-off on behavior during the dry period and clinical mastitis in the subsequent lactation in herd using selective dry cow therapy. Randomized clinical trial investigating the effects of pre dry-off administration of acidogenic bolus on udder health during the dry period and in behavior in the early dry period in herd using selective dry cow therapy. Enrollment started in the summer of 2023 and will go until summer of 2025.
2026. Caixeta, D. Nydam. Comparison of the effectiveness of three commercially available vaccines labeled against coliform mastitis. Randomized controlled trial comparing three commercially available vaccines against coliform mastitis. Cows will be enrolled in the Upper Midwest and the Northeast.
2027. Caixeta, D. Nydam. Milk yield before dry-off: implications for postpartum udder health and dry period behavior in dairy cows. In this multistate observational study, the association between milk production before dry-off and udder health in the subsequent lactation will be investigated in order to re-establish recommendations on milk production at dry-off. Cows started to be enrolled in the Spring of 2024.

Rutgers University; New Jersey Agricultural Experiment

Member: Jeffrey Boyd

This year we will continue to focus on using copper or silver ions to kill or prevent the growth of S. aureus.

University of Missouri

Members: Pamela Adkins, John Middleton

We plan to evaluate a model to induce Staphylococcus chromogenes teat end colonization in heifers.  This will be done to determine if induced S. chromogenes colonization can have protective effects on the mammary gland.

Michigan State University

Member: Pamela Ruegg

We will be using qualitative methods to identify veterinarian and farmer identified barriers to enhanced antimicrobial stewardship programs.

University of Vermont Agriculture Station

Members: Feng-Qi Zhao, John Barlow

Barlow lab will continue to explore the potential role of endogenous inhibitor bacteria and bacteriocins in the epidemiology of Staphylococcus mastitis.

Peer-Reviewed Literature

University of Minnesota

Peña-Mosca F; Dean CJ; Fernandes LM, Doster E, Sharpe K, Tui R, Feijoo V, Antunes A, Baumann C, Wehri T, Heins B, Pinedo P, Machado V, Noyes NR, Caixeta L. Associations between early lactation intramammary infections and udder health and performance during the first 180 days in milk in first-lactation organic dairy cows. Journal of Dairy Science. 2023. https://doi.org/10.3168/jds.2023-23924.

Mosca F, ^§Dean C, Machado V, Fernandes L, Pinedo P, Doster E, Heins B, Sharpe K, Baumann C, Ray T, Feijoo V, Antunes A, Wehri T, Noyes NR, Caixeta L. Investigation of intramammary infections in primiparous cows during early lactation on organic dairy farms. In press at Journal of Dairy Science. https://doi.org/10.3168/jds.2022-23036

Dean, C. , Y. Deng, T. Wehri, T. Ray, F. Peña-Mosca, B. Crooker, S. Godden, L. Caixeta, and N. Noyes. 2023. The impact of kit, environment and sampling contamination on the observed microbiome of bovine milk. mSystems https://doi.org/10.1101/2023.11.07.566052

Brink, A.A. , W.J. Weber, J.D. Lippolis, J.B. Cole, A.K. Rendahl, L. Caixeta, S.M. Godden, A.J. Seykora, and B.A. Crooker. 2023. Effect of Holstein genotype on ex-vivo interleukin-1β response to lipopolysaccharide (LPS), lipoteichoic acid (LTA) and heat-killed Gram-negative and Gram-positive
bacteria. Vet Imm & Immunopathology. 258:110573 https://doi.org/10.1016/j.vetimm.2023.110573

Florentino, C.C. , E. Shepley, M. Ruch, Mahmoud, M.M., L. Tikofsky, W. Knauer, G. Cramer, S. Godden, L. Caixeta. 2023. A randomized clinical trial evaluating the effects of acidogenic boluses administration at dry-off on rumination and activity behavior in the 14 subsequent days. Submitted to JDS Communications 4:293-297. https:// doi.org/10.3168/jdsc.2022-0366

Dean, C.J. , F. Peña-Mosca, T. Ray, T. J. Wehri, K. Sharpe, A.M. Antunes Jr., E. Doster, L. Fernandes, V. F. Calles, C. Bauman, S. Godden, B. Heins, P. Pinedo, V. S. Machado, L. S. Caixeta, N. R. Noyes. 2023. Exploring associations between the teat apex metagenome and Staphylococcus aureus intramammary infection risk in primiparous cows under organic directives. BioRxiv https://doi.org/10.1101/2023.09.12.557435

Daneshi, M., Caton, J.S., Caixeta, L.S., Eftekhari, Z., Ward, A.K. “Expression, regulation, and function of β-defensins in the bovine mammary glands: current knowledge and future perspectives.” Animals. 2023, 13(21), 3372. https://doi.org/10.3390/ani13213372

Dziuba, M., L.S. Caixeta, B. Boyum, S. Godden, E. Royster, S. Rowe. 2023. Negatively controlled trial investigating the effect of dry cow therapy on clinical mastitis and culling in multiparous cows. J. Dairy Sci.  106:5687-5695

Rowe, S., F. Kabera, S. Dufour, S. Godden, J.P. Roy. 2023. Selective dry cow therapy can be implemented successfully in cows of all milk production levels. J. Dairy Sci. 106:1953-1967  https://doi.org/10.3168/jds.2022-22547

Godden, S., E. Royster, B.A. Crooker, J. Timmerman, F. Peña Mosca. 2023. Methods of processing recycled manure solids bedding on Midwest dairy farms I: Associations with bedding bacteria counts, milk quality, udder health and milk production. Bov. Pract. 57(1):10-20.

Brink, A.A., W.J. Weber, J.D. Lippolis, J.B. Cole, S.M. Godden, A. Seykora, B.A. Crooker. 2022. Effect of Holstein genotype on ex-vivo innate immune response to lipopolysaccharide (LPS) and lipoteichoic acid (LTA) during the periparturient period. J. Vet Immunology and Immunopathol. 251:110463.  https://doi.org/10.1016/j.vetimm.2022.11046

Ray, T., T. Gaire, C. Dean, S.M. Rowe, S.M. Godden, N.R. Noyes. 2022. The microbiome of common bedding materials before and after use on commercial dairy farms.  Animal Microbiome (2022) 4: 18 https://doi.org/10.1186/s42523-022-00171-2

Florentino, C.C. ^&; Pena Mosca, F.; Ruch, M.; Shepley, E.; Barbosa Arias, M.; Moreira, D.M.; Mahmoud, M.M.; Tikofsky, L.; Knauer, W.; Cramer, G.; Godden, S.; Caixeta, L.S. “Randomized clinical trial evaluating the effect of administering acidogenic boluses at dry-off on udder health and cow performance.” Journal of Dairy Science. 2024 . https://doi.org/10.3168/jds.2023-23757

Rutgers University; New Jersey Agricultural Experiment

Andrews T.P., Hoyer J.S., Fahrenfeld N.L., Boyd J.M.*, Duffy S.* Complete genome sequences of five Phietaviruses infecting Staphylococcus aureus. Microbiology Resource Announcements 10/2022 PMID: 36173192

Hudspeth, J.D., Boncella A.E., Sabo E.T., Andrews T., Boyd J.M., Morrison C.N., Structural and Biochemical Characterization of Staphylococcus aureus Cysteine Desulfurase Complex SufSU. American Chemical Society Omega. 11/2022 PMID: 36506149

Andrews, T., Ficken K., Fey P.D., Duffy S., Boyd J.M., Novel Transducing Bacteriophage Infecting Staphylococcus epidermidis Contributes to the Expansion of a Novel Siphovirus Genus and Implies Genus is Inappropriate for Phage Therapy. mSphere. 2/2023 PMID: 37017574

Ramírez-Hernández M., Norambuena J., Hu H., Thomas B., Tang C., Boyd J.M.*, Asefa T.* Repurposing Anthelmintics: Effects of Rafoxanide and Copper-Functionalized SBA-15 Carriers Against Methicillin-Resistant Staphylococcus aureus (MRSA). American Chemical Society Applied Materials & Interfaces 3/2023 PMID: 36975176

Norambuena J., Al-Tameemi H., Bovermann H., Kim J., Beavers W.N., Skaar E.P., Parker D., Boyd J.M. Copper ions inhibit pentose phosphate pathway function in Staphylococcus aureus. PLoS Pathogens. 5/2023 PMID: 37235600

Kim G.L., Kim J., Norambuena J., Boyd J.M., Parker D., Impact of the pentose phosphate pathway on metabolism and pathogenesis of Staphylococcus aureus. PLoS Pathogens. 6/2023 PMID: 37440594

Hossain S., Morey J.R., Neville S.L., Ganio K., Radin J.N., Norambuena J., Boyd J.M., McDevitt C.A., Kehl-Fie T.E., Host subversion of bacterial metallophore usage drives copper intoxication. mBio 8/2023 PMID: 37737591

Leanse L.G., dos Anjos C., Ryan Kaler K., Hui J., Boyd J.M., Hooper D.C., Anderson R.R., Dai T., Blue light potentiates antibiotic activity in bacteria via parallel pathways of hydroxyl radical production and enhanced antibiotic uptake. 8/2023 Advanced Science PMID: 37946633

University of Missouri

Kurban D, Roy JP, Kabera F, Fréchette A, Um MM, Albaaj A, Rowe S, Godden S, Adkins PRF, Middleton JR, Gauthier ML, Keefe GP, DeVries TJ, Kelton DF, Moroni P, dos Santos MV, Barkema HW, and Dufour S. 2022. Diagnosing intramammary infection: meta-analysis and mapping review on frequency and udder health relevance of microorganism species isolated from bovine milk samples. Animals. Nov 25; 12(23): 3288. https://doi.org/10.3390/ani12233288

de Jong EC, Creytens, L, De Vliegher S, McCubbin KD, Baptiste M, Leung AA, Speksnijder D, Dufour S, Middleton JR, Ruegg PL, Lam TJGM, Kelton DF, McDougall S, Godden SM, Lago A, Rajala-Schultz PJ, Orsel K, Kromker V, Kastelic JP, Barkema HW.  2023.  Selective treatment of nonsevere clinical mastitis does not adversely affect cure, somatic cell count, milk yield, recurrence, or culling: a systematic review and meta-analysis.  J Dairy Sci.  106(2):1267-1286.  https://doi.org/10.3168/jds.2022-22271

Loy JD, Clawson ML, Adkins PRF, Middleton JR.  2023.  Current and Emerging Diagnostic Approaches to Bacterial Diseases of Ruminants.  Vet Clin North Am Food Anim Pract.  39(1):93-114.  Invited Article.  https://doi.org/10.1016/j.cvfa.2022.10.006

de Jong E, McCubbin KD, Speksnijder D, Dufour S, Middleton JR, Ruegg PL, Lam TJGM, McDougall S, Godden SM, Lago A, Rajala-Schultz PJ, Orsel K, De Vliegher S, Kromker V, Kastelic JP, Barkema HW.  2023.  Invited Review: Selective treatment of clinical mastitis in dairy cattle.  J Dairy Sci.  106(6):3761-3778.  https://doi.org/10.3168/jds.2022-22826

Michigan State University

Kolar, Q., S. Godden, R. Erskine, and P.L. Ruegg.  2024.  SHORT COMMUNICATION:  Confirmed bacteriological diagnosis and clearance of non-severe Gram-positive clinical mastitis cases enrolled in a randomized clinical trial using an on-farm culture program.  J Dairy Sci Comm.  Uncorrected proof:  https://www.jdscommun.org/action/showPdf?pii=S2666-9102%2824%2900086-3

Strickland, J.M., J.L. de Campos, J. Gandy, V. Mavangira, P.L. Ruegg and L. Sordillo. 2024.  A randomized control trial to test the effect of pegbovigrastim treatment at dry off on plasma and milk oxylipid profiles during early mammary gland involution and post-parturient period.  J Dairy Science, available online:  https://doi.org/10.3168/jds.2023-23879

Vasco, K., S.Carbone, R.Mosci, B.Bowcutt, R. Colwell, K.Graubics, R.Erskine, B. Norby, P.L. Ruegg*, L. Zhang*, S.Manning*. 2023. Persistent effects of intramammary ceftiofur treatment on the gut microbiome and antibiotic resistance in dairy cattle.  Animal Microbiome.  5:56 (2023). https://doi.org/10.1186/s42523-023-00274-4  *co-corresponding authors

Pegolo, S., D. Giannuzzi, F. Piccioli-Cappelli, L. Cattaneo, M. Gianesella, P.L. Ruegg, E. Trevisi, and A. Cecchinato.  2023. Blood biochemical changes upon subclinical intramammary infection and inflammation in Holstein cattle.  J Dairy Sci.  106:6539-6550. https://doi.org/10.3168/jds.2022-23155

Pegolo, S., A. Toscanoa, V. Bisutti, A. Vanzin, D. Giannuzzi, L. Gallo, F. Tagliapietra,  P. Ruegg, M. Gianesella, M. Mele, S. Schiavon, and A. Cecchinato.  2023.  Associations between subclinical intramammary infection and milk fatty acid profile at the quarter level in Holstein cattle.  Animal 17(10), 100978.   https://doi.org/10.1016/j.animal.2023.100978

Goncalves, J.L., R. Mani, S. Sreevatsan, and P.L. Ruegg.  Apparent prevalence and selected risk factors of methicillin resistant and susceptible Staphylococcus aureus and non-aureus Staphylococci and Mammaliicocci in bulk tank milk of dairy herds in Indiana, Ohio, and Michigan.  2023.  J. Dairy Sci.Comm. available online:  https://doi.org/10.3168/jdsc.2023-0386

Leite de Campos, J., Goncalves, J.I., J., A. Kates, A. Steinberger, A. Sethi, G. Suen, J. Shutske, N. Safdar, T. Goldberg, and P.L. Ruegg.   2023.  Variation in Partial Direct Costs of Treating Clinical Mastitis among 37 Wisconsin Dairy Farms.  J Dairy Sci 106:9726-9286 https://doi.org/10.3168/jds.2023-23388 

South Dakota State University

Swartz, T. H., and C. S. Petersson-Wolfe. 2023. Short communication: Associations between pre-weaned calf feeding behaviors with age at first calving and lactational performance using an automatic calf feeder. J. Dairy Sci. Comm. 4:75-79. https://doi.org/10.3168/jdsc.2022-0255

Rodriguez, Z., Q. Kolar., K. Krogstad, T. H. Swartz, I. Yoon, B. J. Bradford, and P. Ruegg. 2023. Evaluation of a reticuloruminal temperature for the prediction of clinical mastitis in dairy cows challenged with Streptococcus uberis. J. Dairy Sci. 106:1360-1369. https://doi.org/10.3168/jds.2022-22421

Swartz, T. H., D. M. Bryant, H. H. Schramm, A. J. Duncan, R. R. White, C. M. Wood, and C. S. Petersson-Wolfe. 2023. The effects of dexamethasone administration on physiological, behavioral, and production responses in dairy cows after a difficult calving. J. Dairy Sci. 106:653-663. https://doi.org/10.3168/jds.2022-22029

Swartz, T. H., B. J. Bradford, M. Lemke, L. K. Mamedova, R. Agnew, J. Fehn, E. Owczarzak, J. L. McGill, and K. A. Estes. 2022. Effects of prenatal dietary rumen-protected choline supplementation during late gestation on calf growth, metabolism, and vaccine response. J. Dairy Sci. 105:9639-9651. https://doi.org/10.3168/jds.2022-22239

University of Vermont Agriculture Station

Pengfei Hou; Wenting Dai; Yanshan Jin; Fengqi Zhao; Jianxin Liu, Hongyun Liu. (2022). Maternal exposure to di-2-ethylhexyl phthalate (DEHP) depresses lactation capacity in mice. Science of The Total Environment 837, 1 September 2022, 155813. doi: 10.1016/j.scitotenv.2022.155813

Caihong Wang, Feng-Qi Zhao, Jianxin Liu, and Hongyun Liu. (2022). The ubiquitin ligase Nedd4-2 mediates the regulation of PepT2 by mTORC1 in bovine mammary epithelial cells. Animal Nutrition 10:12-18. doi: 10.1016/j.aninu.2021.11.00

Abstracts

University of Minnesota

Pena Mosca, F., Ruch, M., Florentino, C.C., Mahmoud, M., Godden, S.M., Royster, E., Boyum, B., Rowe, S., Caixeta, L.S. “Negatively controlled trial investigating the effect of dry cow therapy on prevalence of intramammary infections in first lactation dairy cows.” 62nd Annual Meeting of the National Mastitis Council, Atlanta, GA.  January 2023.

Pena Mosca, F.*, Dean, C., Machado, V.S., Fernandes, L., Pinedo, P., Doster, E., Manriquez, D., Heins, B., Sharpe, K., Ray, T., Noyes, N., Caixeta, L.S. “The association between the presence of intramammary infections in the first month of lactation and high somatic cell count during the first six months of lactation in first-lactation organic dairy cows.” 62nd Annual Meeting of the National Mastitis Council, Atlanta, GA. January 2023

Godden, S., E. Royster, E. Leonard, D. Albrecht, J. Timmerman. Pilot Study: Refining a Culture-Guided Selective Dry Cow Therapy Program to Enhance Antimicrobial Stewardship on Dairy Farms. Mastitis Research Workers Conference. Chicago, IL Nov. 2-4, 2022.

Leonard, E., Crooker, B.A., Dow, S., Caixeta, L.S. “Intramammry liposome-TLR agonist (LTC) dose titration: effect on differential somatic cell count.” ADSA Conference, Ottawa, Canada, June 2023.

University of Missouri

Kurban D, Roy JP, DeVries TJ, Adkins PRF, Middleton JR, Keefe GP, France A, and Dufour S.  2023.  Staphylococcal intramammary infections in dairy cows: prevalence at calving in first lactation cows and prevalence, incidence and persistence over the dry period.   American Dairy Science Association.

Kurban D, Roy JP, DeVries TJ, Adkins PRF, Middleton JR, Keefe GP, France A, and Dufour S.  2023.  Staphylococcal intramammary infections in dairy cows: prevalence, incidence and persistence over the dry period.  Canadian Association of Veterinary Epidemiology and Preventive Medicine.  University of Guelph, Ontario.  31 May 23 – 1 Jun 23.

Isensee PK and Adkins PRF. Characterization of antibacterial activity of Staphylococcus chromogenes isolates originating from dairy cattle. MU CVM Research Day. May 5, 2023.

Novo A and Adkins PRF. Intramammary infections in primiparous dairy heifers. MU CVM Research Day. May 5, 2023.

Michigan State University

Kolar, Q.K., K.C. Krogstad, V. Mavangira, T.H. Swarz, I. Yoon, B. J. Bradford and P.L. Ruegg. 2023.  Effects of feeding with Saccharomyces cerevisiae fermentation product on milk oxylipids prior to and during an intramammary S. uberis challenge.  Presented at Ann. Meeting Am. Dairy Sci Assoc., July 2023, Montreal, Canada.

Bacigalupo-Sanguesa, P.L. Ruegg, R. Thomson, B. Norby, A. Contreras and R. Erskine.  2023.  Long Term Impact of Delayed Milk Ejection on Milk Production.  Pp 129-130 in Proceedings of the 62nd Annual M  eeting of the NMC, Jan 30-Feb 2, Atlanta GA.

Bacigalupo-Sanguesa, P, P.L. Ruegg, R. Thomson, B. Norby, A. Contreras and R. Erskine.  2023.  Identificaion of delayed milk ejection episodes using milk flow recordings. Pp 141-142 in Proceedings of the 62nd Annual Meeting of the NMC, Jan 30-Feb 2, Atlanta GA.

Goncalves, J., R. Mani, Q. Kolar, S. Sreevatsan, and P.L. Ruegg.  2023.  Distribution of minimum inhibitory concentrations of selected antibiotics for mecA Positive and negative Non-aureus Staphylococci that exhibit phenotypic resistance to methicillin.   Pp 169-170 in Proceedings of the 62nd Annual Meeting of the NMC, Jan 30-Feb 2, Atlanta GA.

Kolar, Q.K, J. L. Goncalves, S.M. Godden,  R. J. Erskine, and P.L. Ruegg.  Comparison of in-vitro resistance of Non-aureus Staphylococci, Enterococci, Lactococci, and other Strep-like organism to approved intramammary antimicrobials.  Pp 161-162 in Proceedings of the 62nd Annual Meeting of the NMC, Jan 30-Feb 2, Atlanta GA.

Rodriguez, Z., V.E. Cabrera, H. Hogeveen, and and P. L. Ruegg.  2023.  Economic simulation to evaluate the cost-effectiveness of subclinical mastitis treatment using nisin during early lactdation – preliminary results.  Pp 133-134 in Proceedings of the 62nd Annual Meeting of the NMC, Jan 30-Feb 2, Atlanta GA.

Pinzon-Sanchez, C., P. Bacigalupo, M. Buza, P. Durst, M. Mangual, V. Malacco, and P.L. Ruegg.  2023.  Benchmarking Antibiotic Usage:   A Practical Online Tool. Pp 167-168 in Proceedings of the 62nd Annual Meeting of the NMC, Jan 30-Feb 2, Atlanta GA.

South Dakota State University

Kolar, Q. K., K. C. Krogstad, V. Mavangira, T. H. Swartz, I. Yoon, B. J. Bradford, and P. L. Ruegg. Effects of supplementation with Saccharomyces cerevisiae fermentation product on milk oxylipids prior to and during an intramammary challenge with S. uberis. American Dairy Science Association. Ottawa, Ontario. June 25-28, 2023.

Swartz, T. H., B. J. Bradford, L. K. Mamedova, and K. A. Estes. Effects of dietary rumen-protected choline supplementation on choline metabolites and inflammatory markers in mammary tissue during an intramammary lipopolysaccharide challenge. American Dairy Science Association. Ottawa, Ontario. June 25-28, 2023.

Swartz, T. H., B. J. Bradford, L. K. Mamedova, and K. A. Estes. Effects of dietary rumen-protected choline supplementation on the plasma metabolome during an intramammary lipopolysaccharide challenge. American Dairy Science Association. Ottawa, Ontario. June 25-28, 2023.

Kolar, Q. K., K. C. Krogstad, T. H. Swartz, I. Yoon, P. Coussens, M. Bernard, V. Mavangira, B. J. Bradford, and P. L. Ruegg. Effect of supplementation with Saccharomyces cerevisiae fermentation product on mammary immune cell populations. Conference of Research Workers in Animal Diseases. Chicago, IL. Jan 20-24, 2023.

Rodriguez, Z., Q. Kolar, K. Krogstad, T. H. Swartz, I. Yoon, B. J. Bradford, and P. L. Ruegg. Evaluation of reticuloruminal temperature for prediction of clinical mastitis in dairy cows challenged with Streptococcus uberis. Conference of Research Workers in Animal Diseases. Chicago, IL. Jan 20-24, 2023.

Conference Proceedings

University of Missouri

Lopez-Benavides M, Haw S, Britt L, Partridge J, Leibowitz J, Middleton J.  2023.  Teat canal measurements around milking using ultrasound.  Proceedings of the 62nd Annual Meeting of the National Mastitis Council.  Atlanta, Georgia.  30 Jan 2023 – 2 Feb 2023.

Middleton JR, Adkins PRF.  2023. The What, Why, When and How of Mastitis Treatment.  Short course #7 presented at 62nd Annual Meeting of the National Mastitis Council.  Atlanta, Georgia.  31 Jan 2023.  Electronic Note Set.

Adkins PRF.  Antimicrobial use for the treatment of bovine mastitis. American College of Veterinary Internal Medicine Annual Forum (Live Virtual Day) June 28, 2023.

Isensee PK and Adkins PRF.  Characterization of antibacterial activity of Staphylococcus chromogenes isolates originating from dairy cattle. Proceedings of the 61st Annual Meeting of the National Mastitis Council, Atlanta, Georgia. Jan 30- Feb 2, 2023.

Michigan State University

Ruegg, P.L.  Clinical mastitis treatment decisions.   2023. In Proc. 6th Am. Assoc. Bov. Pract. Recent Graduate Conf. Knoxville TN, Feb 10-13, 2023

Rodriguez, Z., V.E. Cabrera, H. Hogeveen, and and P. L. Ruegg.  2023.  Economic simulation to evaluate the cost-effectiveness of subclinical mastitis treatment using nisin during early lactdation – preliminary results.  Pp 133-134 in Proceedings of the 62nd Annual Meeting of the NMC, Jan 30-Feb 2, Atlanta GA.

Pinzon-Sanchez, C., P. Bacigalupo, M. Buza, P. Durst, M. Mangual, V. Malacco, and P.L. Ruegg.  2023.  Benchmarking Antibiotic Usage:   A Practical Online Tool. Pp 167-168 in Proceedings of the 62nd Annual Meeting of the NMC, Jan 30-Feb 2, Atlanta GA.

Steinberger, A., Leite de Campos, J., T. Goldberg, P. Ruegg, N. Safdar, J. Sutske, A. Sethi, and G. Suen.  Investigating the role of dairy farm antimicrobial use on the bovine fecal resistome.  Presented at Conf. Research Workers Animal Disease, Chicago IL, Jan 2023

Rodriguez, Z., Q.K. Kolar, K.C. Krogstad,T. H. Swartz, I.Yoon, B. J. Bradford, and P. L. Ruegg.  2023.  Evaluation of reticuloruminal temperature for prediction of clinical mastitis in dairy cows challenged with Streptococcus uberis. Presented at Conf. Research Workers Animal Disease, Chicago IL, Jan 2023

Goncalves, J., Mani, R., Kolar, Q., Sreevatsan, S. and P.L. Ruegg.  2023.  Prevalence of methicillin-resistant and susceptible Staphylococci spp., in bulk tank milk from dairy herds in Michigan. Presented at Conf. Research Workers Animal Disease, Chicago IL, Jan 2023

Utah State University

Wilson DJ, Goodell GM, Dumm R, Kelly T, Bethard M: Sodium percarbonate as a preservative in waste milk fed to dairy calves. Proc 74th Ann Conf European Federation of Animal Science (EAAP): 534, 2023.

Wilson DJ, Goodell GM, Dumm R, Kelly T, Bethard M: A preservative for waste/diverted milk fed to dairy calves.  Mast Res Workers, Ghent, Belgium, 2024

Poster Presentations

Rutgers University; New Jersey Agricultural Experiment

Esquilín-Lebrón K., Foley M., Carabetta V., Beavers W., Skaar E., and  Boyd J.M.  YlaN functions in iron homeostasis in Staphylococcus aureus. Society for Advancement of Chicanos/Hispanics and Native Americans in Science Annual Meeting. Virtual 10/2021.

Ficken K. and Boyd J.M.  Investigating Bacteriophages as a Novel Therapeutic Approach against Common Skin-Infecting Bacteria. Aresty research symposium, Rutgers University, New Brunswick, NJ 3/2022.

McGinley C.M. and Boyd J.M.  Investigation into the roles of conserved amino acids for the function of the metal ion homeostasis protein YlaN. Aresty research symposium, Rutgers University, New Brunswick, NJ 3/2022.

Rios-Delgado G., Boyd J.M.  The role of the small RNA tsr25 in Staphylococcus aureus iron homeostasis Rutgers Initiative for Maximizing Student Development (IMSD) symposium, New Brunswick, NJ 5/2022.

Rios-Delgado G., Boyd J.M.  The role of the small RNA tsr25 in Staphylococcus aureus iron homeostasis New Jersey American Society of Microbiology Meeting in Miniature (Theobald Smith Society). New Brunswick, NJ 5/2022.

Román-Rodríguez F. and Boyd J. M.  Examining the stimuli of the SrrAB regulatory system in Staphylococcus aureus. New Jersey American Society of Microbiology Meeting in Miniature (Theobald Smith Society). New Brunswick, NJ 5/2022.

Rios-Delgado G., Boyd J.M.  The role of the small RNA tsr25 in Staphylococcus aureus iron homeostasis New Jersey American Society of Micrbiology Meeting in Miniature (Theobald Smith Society). New Brunswick, NJ 5/2022

Norambuna J. and Boyd J.M.  Cu ion homeostasis in Staphylococcus aureus New York Bacillus Interest Group (NYBIG) annual conference, New York, NY. 6/2022.

Ranainghe, L., Ryan Kaler K., Boyd J.M. Functional Assessment of the Regulon of the Ferric Uptake Regulator (Fur) in Staphylococcus aureus Aresty research symposium, Rutgers University, New Brunswick, NJ 3/2023.

Ranainghe, L., Ryan Kaler K., J.M. Boyd Functional Assessment of the Regulon of the Ferric Uptake Regulator (Fur) in Staphylococcus aureus Rutgers Microbiology symposium, Rutgers University, New Brunswick, NJ 4/2023.

Rios-Delgado G., Norambuena J., Ryan Kaler K., Briaud P., Mustor E., Shaw L., Parker D., Carrol R., J.M. Boyd. The role of Tsr25 in iron homeostasis in Staphylococcus aureus. Rutgers Microbiology symposium, Rutgers University, New Brunswick, NJ 4/2023.

Roman Rodriguez F. and Boyd J.M. Examining the effects of Pseudomonas aeruginosa secondary metabolites on Staphylococcus aureus transcriptional networks. Rutgers Microbiology symposium, Rutgers University, New Brunswick, NJ 4/2023.

Roman Rodriguez F. J.M. Boyd. Examining the effects of Pseudomonas aeruginosa secondary metabolites on Staphylococcus aureus transcriptional networks. New York Bacillus Interest Group (NYBIG) annual conference, New York, NY. 6/2023.

Rios-Delgado G., Norambuena J., Ryan Kaler K., Briaud P., Mustor E., Shaw L., Parker D., Carrol R., J.M. Boyd. Genetic regulation of iron homeostasis in Staphylococcus aureus. Gordon Research Conference on Staphylococcal Diseases. Waterville Valley, NH 7/2023.

Paola Garay Maris, Gustavo Rios, J.M. Boyd. Staphylococcus aureus iron usage: characterization of a novel antibiotic target. RISE at Rutgers annual conference, New Brunswick, NJ. 8/2023

Paola Garay Maris, Gustavo Rios, J.M. Boyd. Staphylococcus aureus iron usage: characterization of a novel antibiotic target. Intra-College Experience in Creative Research in Experimental and Applied Microbiomics, Rutgers University, New Brunswick, NJ. 8/2023.

University of Missouri

Adkins PRF and Novo A. Evaluation of intramammary infection status in dairy heifers using two different sample collection techniques. Conference of Research Workers in Animal Diseases. Chicago, IL. Jan 20-24, 2023.

Outreach Presentations

Rutgers University; New Jersey Agricultural Experiment

Copper ion homeostasis in Staphylococcus aureus. Rutgers University Dept. of Biochemistry and Microbiology. Virtual 10/2021.

Studying Staphylococcus aureus physiology to decrease disease burden. Rutgers University Molecular Biology and Biochemistry club.  Virtual 3/2022.

Metal ion homeostasis in Staphylococcus aureus. Chemistry Department, Fordham University. 10/2022.

Genetic regulation of iron homeostasis in Staphylococcus aureus. International conference on iron-sulfur proteins—Biogenesis, Regulation, and Function. Saint Tropez, FR 8/2022.

Mechanisms of copper ion intoxication and detoxification in Staphylococcus aureus. Louisiana State University, Dept of Pathobiological Sciences 11/2022.

Metal ion homeostasis in Staphylococcus aureus. Rutgers School of Dental Medicine. Newark, NJ 2/2023.

Copper ions and the Staphylococcus aureus host interface. International Conference of Staphylococcus aureus and Staphylococcal Infections. Virtual 2/2023.

Copper ions and the Staphylococcus aureus host interface. International Conference of Staphylococcus aureus and Staphylococcal Infections. Southern Illinois Medical School, Springfield, IL 2/2023.

Iron homeostasis in Staphylococcus aureus. University of Illinois, Urbana, IL 9/2023.