Brief Summary of Minutes of Annual Meeting

W4112 Multistate Meeting 2024 Minutes

Madison, Wisconsin

May 21-22, 2024

Chairman - Becky Poole (TAMU)

Secretary - David Grieger (K-State)

Member-at-Large - Jessica Drum (SDSU)

Tuesday, May 21

In attendance virtually:

1. Anna Denicol – University of California-Davis
2. Karl Kerns - Iowa State University
3. Rocio Rivera - University of Missouri
4. Jennifer Hernandez Gifford - New Mexico State University
5. Claire Stenhouse - Penn State University
6. Ky Pohler - Texas A&M University
7. Becky Poole - Texas A&M University

In attendance, In -Person:

1. Rick McCosh, Colorado State University
2. Kristen Govoni, University of Connecticut
3. Mario Binelli, University of Florida
4. David Grieger, Kansas State University
5. Andrea Cupp, University of Nebraska
6. Ryan Ashley, New Mexico State University
7. Michelle Kutzler, Oregon State University
8. Wansheng Liu, Penn State University
9. Jessica Nora Drum, South Dakota State University
10. Sofia Ortega, University of Wisconsin
11. Brenda Alexander, University of Wyoming
12. Jeremy Block, University of Wyoming

8:20 am – Business meeting called to order by Becky Poole

• Round table introductions for all present (virtual and in-person)
• 2023 meeting minutes approval - Brenda Alexander moved to approve, Andrea Cupp second.
• Location for 2024 meeting - David Grieger nominated Connecticut, Andrea nominated South Dakota (Jessica declined). Vote for location was tabled until tomorrow.
• New officer election- Andrea moved for Jeremy Block to be Member-at-Large and Brenda second. Vote for Jeremy was unanimous and motion carried. Officers for 2025 are David Grieger-Chair, Jessica Drum-Secretary, and Jeremy Block Member-at-Large.

8:30 am - Began STATION Updates

1. Ky Pohler - Texas A&M University
2. Karl Kerns - Iowa State University
3. Mario Binelli, University of Florida
4. Sofia Ortega, University of Wisconsin
5. Kristen Govoni, University of Connecticut
6. Michelle Kutzler, Oregon State University
7. Wansheng Liu, Penn State University
8. Jeremy Block, University of Wyoming
9. Ryan Ashley, New Mexico State University
10. Rick McCosh, Colorado State University
11. Jessica Nora Drum, South Dakota State University

10:00 am - USDA update, Dr. Mark Mirando and Dr. Kamilah Grant

10:40pm – Station Updates continued:

12. Andrea Cupp, University of Nebraska

11:10 am - Began STATION RESEARCH Reports

1 – Nebraska (Andrea Cupp)

2 - New Mexico State (Ryan Ashley, Jennifer Hernandez Gifford)

3 – Connecticut (Kristen Govoni)

12:00 pm – Break for working lunch

4 – Texas (Becky Poole)

5 – South Dakota (Jessica Drum)

1:30 pm Tour of Dairy and Meats Lab, U of WI

2:30 pm Resume Station Reports

6 - Missouri (Rocio Rivera)

7 - Oregon (Michelle Kutzler)

8 - Penn State (Claire Stenhouse, Wansheng Liu)

9 - California (Anna Denicol)

10 - Florida (Mario Binelli)

11 - Colorado (Rick McCosh)

12 - Iowa (Karl Kerns)

13 - Wisconsin (Sofia Ortega)

14 - Kansas (David Grieger)

5:30 pm – Adjourn Meeting

6:00 pm - Dinner at Union Terrace on the lake.

Wednesday, May 22

7:00am – Depart Hotel for tour of Arlington Dairy and Swine units, U. of WI.

10:30am - Station Reports Continued:

15 - Ky Pohler - Texas A&M University

16 - Jeremy Block, University of Wyoming, Brenda Alexander

11:30 am - Business Meeting:

• Voted on Location for 2025 meeting: Connecticut for 2025.
• Date for meeting to be determined – A poll will be sent out in early fall to find best dates for attendance for all.
• Storrs airport - BDL. Boston and NY airports are 2 hours away.

Brenda offered to put together the publication list for this report to make it compatible for the next re-write. 

12:05pm - Meeting adjourned

Accomplishments

Outputs form the group during this reporting period

1. 83 peer-reviewed manuscripts
2. 79 published abstracts
3. 8 invited oral presentations
4. 10 thesis/dissertation

Activities

Objective 1: Elucidate fundamental cellular, physiological, endocrine, and behavioral mechanisms that regulate gamete development and quality and enhance the management of reproductive function leading to the development of translational reproductive biotechnologies.

1. We showed that bovine sperm capacitation biomarkers such as zinc signatures can predict embryo development rates better than traditional sperm motility traits alone. This can aid IVF practitioners in selecting sires to enhance reproductive success. Iowa
2. Understanding the roles that FSH may play in the development of preantral follicles is of critical importance as many ovarian stimulation protocols used in assisted reproduction use FSH to promote antral follicle growth. California
3. Knowledge gained from studying the PRAME/PRAMEY gene family will improve our understanding of the molecular mechanisms underlying spermatogenesis, oogenesis, sperm-egg binding and fertilization, as well as early embryo development, which in turn, will help us address issues related to male subfertility and infertility. Pennsylvania
4. We are now able to identify open cows with 95% accuracy as early as day 17 following artificial insemination in dairy and beef cows. The benefit to both dairy and beef cow producers’ centers on the savings of 2-3 weeks to maintain the open cow prior to re-breeding following first insemination. Colorado
5. The corpus luteum is a dynamic endocrine gland that produces the progesterone that is critical for a successful pregnancy. We have shown that a 3-D corpus luteum organoid culture produces more progesterone than luteal cell primary cultures and may be a better overall system to understand mechanisms that occur in the corpus luteum during luteal regression and in developing vasculature than our 2D models. Nebraska
6. We have shown that bacterial endotoxin (LPS) within the ovarian follicle may contribute to decreased reproductive efficiency that may impact oocyte quality and subsequent embryo development. New Mexico

Objective 2: Identify impacts of reproductive management, animal management, and stress on follicle recruitment, ovulation, corpus luteum function, and pregnancy.

7. We are working to investigate flax seed-based supplement effects on reproductive physiology in beef cattle including follicular dynamics, embryo production, super ovulatory response, and circulating progesterone. South Dakota
8. We are working to develop a CRISPR/SaCas9 approach to better understand deletion of ESR1 gene in adult neural tissue which will enable us to conduct experiments to understand the neural regulation of reproduction. Colorado
9. Sex steroid concentrations modulate endometrial function and fertility in cattle. We have identified that contrasting concentrations of progesterone before luteolysis alters the post-estrus uterine luminal transcriptome in beef cattle. Estrus synchronization strategies considering concentrations of progesterone may impact the pregnancy outcome. Florida
10. We have determined that our pubertal classifications are moderately to highly heritable and associated with candidate genes involved in puberty, cyclicity and hormone secretion. This may allow for future investigation into genomic tools that may be used to cull or retain heifers in the herd. Nebraska
11. We initiated a study of the vaginal microbiome during a long estrus synchronization protocol such as the 7&7 and its effects on fertility in beef cattle. South Dakota
12. There is a potential role of the reproductive microbiome in impacting pregnancy status because of hormone concentrations and breeding strategies. Vaginal bacterial diversity appears to shift between the day of artificial insemination and day of maternal recognition of pregnancy in lactating dairy cattle. Texas
13. Altering the bovine vaginal microbiome via betadine lavages near parturition increased maternal immune responses without altering neonatal microbial communities. Mississippi
14. Dysregulation of the estrogen receptor 1 (ESR1) expression during the establishment of pregnancy could contribute to reduced fertility reported in ewes treated with PG-600. Although progesterone was higher on day 7, ESR1 did not differ from controls suggesting that treatment with PG600 is unlikely to impair reproductive potential. Oregon
15. Rumen protected omega-6 (ω-6) fatty acids may improve health and reproductive success in beef cattle. Free choice ω-6 fatty acids supplementation during late gestation and post-artificial insemination did not markedly alter reproductive measures in mature beef cows. Idaho
16. Using heterospermic insemination comparisons may indicate which artificial insemination sires are more fertile for timed-insemination protocols. Kansas

Objective 3: Determine mechanisms regulating normal embryo development, pregnancy establishment, and maintenance by exploring maternal and paternal factors; including genomics, immune responses, fetal programming, and conceptus/uterine signaling.

17. Across mammalian species, phosphate is transported from the mother to the conceptus to allow appropriate regulation of conceptus growth and development. We are working to better understand the mechanisms regulating utero-placental phosphate availability. Pennsylvania
18. We are working to understand the impacts of undernutrition on conceptus development in beef cattle and placental cotyledon development and function in ewes. Montana
19. Current culture systems do not provide a dynamic in vitro environment that mimics the uterine environment during early gestation, thus producing lower quality embryos compared to their in vivo-produced counterparts. We are working to develop bovine uterine organoids that may be used to improve in vitro embryo production in cattle. Montana
20. Disrupting the CXCL12/CXCR4 axis at the fetal-maternal interface during implantation results in an altered hematological profile in circulation indicative of an inflammatory response. Also, the addition of the chemokine CXCL12 at the time of ET influences pregnancy establishment and fetal growth in sheep. New Mexico
21. Treating embryos with FGF2, LIF, and IGF1 improved development to the blastocyst stage, increased the expression of genes associated with placental development, and overall increased the number of embryos eligible for transfer. Wisconsin
22. The functional ablation of PAG7 does not affect blastocyst formation but does delay the time of attachment of the embryo to the extracellular matrix and hinders trophectoderm growth in vitro. Wisconsin
23. Blastocyst development following culture of bovine embryos in the presence of oviductal cell culture supernatant treated with IL-1β was evaluated. While blastocyst development from total oocytes was not affected, culture of embryos with IL-1β resulted in a greater proportion of cleaved embryos developing to the blastocyst stage. Wyoming
24. Intrauterine administration of recombinant bovine interleukin-1 beta during the peri-ovulatory period does not affect subsequent development of the corpus luteum, nor conceptus survival following transfer of bovine embryos produced in vitro. Wyoming
25. Better understanding of the roles of polyamines in implantation, placentation, and conceptus development in beef cattle. Texas
26. We have observed decreased LGALS14 transcript in day 16 conceptuses of low fertility bulls; however, the function of this gene is unknown in cattle. The downregulation of LGALS14 is not caused by differential DNA methylation in the proximal promoter region between low and high fertility bulls. Missouri
27. We characterized the methylome, transcriptome and chromatin configuration of Beckwith-Wiedemann Syndrome (BWS) individuals together with the animal model of the condition, the bovine large offspring syndrome (LOS). Altered chromosome compartments in BWS and LOS were positively correlated with gene expression changes. Missouri
28. Treating embryo recipient cows with pooled seminal plasma negatively impacts early gestation conceptus growth and mid- to late-gestation uterine artery resistance suggesting downstream vascular anomalies; however, these in utero conditions do not appear to impact offspring in their postnatal lives. Mississippi
29. Decrease antioxidant activities and increased products of protein and lipid oxidation in skeletal muscle of offspring from poorly nourished dams suggests dysregulation of oxidative status which may contribute to the negative consequences observed in lamb offspring. Connecticut
30. Poor maternal nutrition during gestation in ewes alters oxidative status in F1 and F2 offspring. Connecticut

1. Abedal-Majed M.A., Abuajamieh M., Al-Qaisi M., Sargent K.M., Titi H.H., Alnimer M.A., Abdelqader A., Shamoun A.I. & Cupp A.S. (2023) Sheep with ovarian androgen excess have fibrosis and follicular arrest with increased mRNA abundance for steroidogenic enzymes and gonadotropin receptors. J Anim Sci 101.
2. Ashley R.L., Trigo E.M. & Ervin J.M. (2023) Placental insufficiency and heavier placentas in sheep after suppressing CXCL12/CXCR4 signaling during implantationdagger. Biol Reprod 109, 982-93.
3. Biase F.H., Moorey S.E., Schnuelle J.G., Rodning S., Ortega M.S. & Spencer T.E. (2023) Extensive rewiring of the gene regulatory interactions between in vitro-produced conceptuses and endometrium during attachment. PNAS Nexus 2, pgad284.
4. Brown W., Oliveira M., Silva R.R., Demetrio D. & Block J. (2024a) Effects of administration of mycobacterium cell wall fraction during the periovulatory period on embryo development following superovulation in virgin dairy heifers. JDS Communications.
5. Brown W., Oliveira M., Silva R.R., Woodruff K., Bisha B., Demetrio D. & Block J. (2024b) Effects of mycobacterium cell wall fraction on embryo development following in vitro embryo production and pregnancy rates following embryo transfer in virgin dairy heifers. Theriogenology 215, 334-42.
6. Cain J.W., Seo H., Bumgardner K., Lefevre C., Burghardt R.C., Bazer F.W. & Johnson G.A. (2024) Pig conceptuses utilize extracellular vesicles for IFNG-mediated paracrine communication with the endometrium. Biol Reprod.
7. Castro B., Candelaria J.I., Austin M.M., Shuster C.B., Gifford C.A., Denicol A.C. & Hernandez Gifford J.A. (2024) Low-dose lipopolysaccharide exposure during oocyte maturation disrupts early bovine embryonic development. Theriogenology 214, 57-65.
8. Cavalcante de Souza D., Gonella-Diaza A.M., de Carvalho N.A.T., Elliff F.M., de Carvalho J.G.S., Vieira L.M., Bonfim-Neto A.P., de Carvalho Papa P., Ghuman S.S., Madureira E.H., Pugliesi G., Binelli M. & Baruselli P.S. (2024) Supplementation with long-acting injectable progesterone 3 days after TAI impaired luteal function in buffaloes. Trop Anim Health Prod 56, 76.
9. Contreras-Correa Z.E., Sánchez-Rodríguez H.L., Arick II M.A., Muñiz-Colón G. & Lemley C.O. (2024) Thermotolerance capabilities, blood metabolomics and mammary gland hemodynamics and transcriptomic profiles of slick-haired Holstein cattle during mid-lactation in Puerto Rico. Journal of Dairy Science.
10. Cortes-Araya Y., Cheung S., Ho W., Stenhouse C., Ashworth C.J., Esteves C.L. & Donadeu F.X. (2024) Effects of foetal size, sex and developmental stage on adaptive transcriptional responses of skeletal muscle to intrauterine growth restriction in pigs. Sci Rep 14, 8500.
11. Davenport K.M., O’Neil E.V., Ortega M.S., Patterson A., Kelleher A.M., Warren W.C. & Spencer T.E. (2023a) Single-cell insights into development of the bovine placenta†. Biology of Reproduction 110, 169-84.
12. Davenport K.M., Ortega M.S., Johnson G.A., Seo H. & Spencer T.E. (2023b) Review: Implantation and placentation in ruminants. Animal 17 Suppl 1, 100796.
13. Denicol A.C. & Siqueira L.G.B. (2023) Maternal contributions to pregnancy success: from gamete quality to uterine environment. Anim Reprod 20, e20230085.
14. Diaz-Miranda E.A., Hamilton L.E., Zigo M., Fallon L., Ortega M.S., Assumpção M.E.O.D.A., Guimarães J.D. & Sutovsky P. (2024) Regional abundances of binder of sperm (BSP) proteins are negatively associated with the quality of frozen-thawed bovine spermatozoa. Reproduction 167.
15. Fallon L., Diaz-Miranda E., Hamilton L., Sutovsky P., Zigo M., Spencer T.E. & Ortega M.S. (2023) The development of new biomarkers of spermatozoa quality in cattle. Frontiers in Veterinary Science 10, 1258295.
16. Geisert R.D., Bazer F.W., Lucas C.G., Pfeiffer C.A., Meyer A.E., Sullivan R., Johns D.N., Sponchiado M. & Prather R.S. (2024) Maternal recognition of pregnancy in the pig: A servomechanism involving sex steroids, cytokines and prostaglandins. Anim Reprod Sci 264, 107452.
17. Griffin C.K., Lemley C.O., Pohler K.G., Sun X. & Lear A.S. (2024) Characterization of placentome vascular perfusion in relation to pregnancy associated glycoproteins throughout gestation in pregnant beef heifers. Theriogenology 219, 94-102.
18. Habeeb H.M.H., Kleditz L., Hazzard T., Bishop C., Stormshak F. & Kutzler M.A. (2023) Ovine endometrial estrogen receptor expression is altered following PG-600 administration. Vet Med Sci 9, 1379-84.
19. Halloran K.M., Stenhouse C., Moses R.M., Kramer A.C., Sah N., Seo H., Lamarre S.G., Johnson G.A., Wu G. & Bazer F.W. (2023) The ovine conceptus utilizes extracellular serine, glucose, and fructose to generate formate via the one carbon metabolism pathway. Amino Acids 55, 125-37.
20. He W., Posey E.A., Steele C.C., Savell J.W., Bazer F.W. & Wu G. (2023) Dietary glycine supplementation enhances postweaning growth and meat quality of pigs with intrauterine growth restriction. J Anim Sci 101.
21. He W., Posey E.A., Steele C.C., Savell J.W., Bazer F.W. & Wu G. (2024) Dietary glycine supplementation activates mTOR signaling pathway in tissues of pigs with intrauterine growth restriction. J Anim Sci.
22. Heredia D., Ojeda-Rojas O.A., Londoño M.C., Lasso S.D., Bisinotto R.S., Binelli M. & Gonella-Diaza A.M. (2023) A single dose of FSH or hCG during a split-time AI program did not enhance follicular growth or pregnancy per artificial insemination in beef heifers. Journal of Applied Animal Research 51, 434-40.
23. Hong T., Park S., An G., Bazer F.W., Song G. & Lim W. (2024) Norflurazon causes cell death and inhibits implantation-related genes in porcine trophectoderm and uterine luminal epithelial cells. Food Chem Toxicol 186, 114559.
24. Hu S., He W., Bazer F.W., Johnson G.A. & Wu G. (2023a) Synthesis of glycine from 4-hydroxyproline in tissues of neonatal pigs. Exp Biol Med (Maywood) 248, 1206-20.
25. Hu S., He W., Bazer F.W., Johnson G.A. & Wu G. (2023b) Synthesis of glycine from 4-hydroxyproline in tissues of neonatal pigs with intrauterine growth restriction. Exp Biol Med (Maywood) 248, 1446-58.
26. Husnain A., Arshad U., Zimpel R., Schmitt E., Dickson M.J., Perdomo M.C., Marinho M.N., Ashrafi N., Graham S.F., Bishop J.V., Hansen T.R., Jeong K.C., Gonella-Diaza A.M., Chebel R.C., Sheldon I.M., Bromfield J.J. & Santos J.E.P. (2023) Induced endometrial inflammation compromises conceptus development in dairy cattledagger. Biol Reprod 109, 415-31.
27. Jara T.C., Park K., Vahmani P., Van Eenennaam A.L., Smith L.R. & Denicol A.C. (2023) Stem cell-based strategies and challenges for production of cultivated meat. Nat Food 4, 841-53.
28. Johnson G.A., Bazer F.W., Seo H., Burghardt R.C., Wu G., Pohler K.G. & Cain J.W. (2023a) Understanding placentation in ruminants: a review focusing on cows and sheep. Reprod Fertil Dev 36, 93-111.
29. Johnson G.A., Burghardt R.C., Bazer F.W., Seo H. & Cain J.W. (2023b) Integrins and their potential roles in mammalian pregnancy. J Anim Sci Biotechnol 14, 115.
30. Johnson G.A., Seo H., Bazer F.W., Wu G., Kramer A.C., McLendon B.A. & Cain J.W. (2023c) Metabolic pathways utilized by the porcine conceptus, uterus, and placenta. Mol Reprod Dev 90, 673-83.
31. Keller A. & Kerns K. (2023) Sperm capacitation as a predictor of boar fertility. Molecular Reproduction and Development 90, 594-600.
32. Kern C., Wu W., Lu C., Zhang J., Zhao Y., Ocon-Grove O.M., Sutovsky P., Diaz F. & Liu W.S. (2023) Role of the bovine PRAMEY protein in sperm function during in vitro fertilization (IVF). Cell Tissue Res 391, 577-94.
33. Ketchum J.N., Perry G.A., Quail L.K., Epperson K.M., Ogg M.A., Zezeski A.L., Rich J.J.J., Zoca S.M., Kline A.C., Andrews T.N., Ortega M.S., Smith M.F. & Geary T.W. (2023) Influence of preovulatory estradiol treatment on the maintenance of pregnancy in beef cattle receiving in vivo produced embryos. Anim Reprod Sci 255, 107274.
34. Li X., Bazer F.W., Johnson G.A., Burghardt R.C. & Wu G. (2023a) Dietary supplementation with L-citrulline improves placental angiogenesis and embryonic survival in gilts. Exp Biol Med (Maywood) 248, 702-11.
35. Li Y., Xiao P., Boadu F., Goldkamp A.K., Nirgude S., Cheng J., Hagen D.E., Kalish J.M. & Rivera R.M. (2023b) The counterpart congenital overgrowth syndromes Beckwith-Wiedemann Syndrome in human and large offspring syndrome in bovine involve alterations in DNA methylation, transcription, and chromatin configuration. medRxiv.
36. Lockhart K.N., Drum J.N., Balboula A.Z., Spinka C.M., Spencer T.E. & Ortega M.S. (2023) Sire modulates developmental kinetics and transcriptome of the bovine embryo. Reproduction 166, 337-48.
37. Lockhart K.N., Fallon L.C. & Ortega M.S. (2024) Paternal determinants of early embryo development. Reproduction, Fertility and Development 36, 43-50.
38. Martinez R.E., Leatherwood J.L., Bradbery A.N., Paris B.L., Hammer C.J., Kelley D., Bazer F.W. & Wu G. (2023) Evaluation of dietary arginine supplementation to increase placental nutrient transporters in aged mares. Transl Anim Sci 7, txad058.
39. Messman R.D. & Lemley C.O. (2023) Bovine neonatal microbiome origins: a review of proposed microbial community presence from conception to colostrum. Transl Anim Sci 7, txad057.
40. Monaco C.F., Plewes M.R., Przygrodzka E., George J.W., Qiu F., Xiao P., Wood J.R., Cupp A.S. & Davis J.S. (2023) Basic fibroblast growth factor induces proliferation and collagen production by fibroblasts derived from the bovine corpus luteumdagger. Biol Reprod 109, 367-80.
41. Moses R.M., Stenhouse C., Halloran K.M., Sah N., Hoskins E.C., Washburn S.E., Johnson G.A., Wu G. & Bazer F.W. (2024a) Metabolic pathways for glucose and fructose: I synthesis and metabolism of fructose by ovine conceptuses. Biol Reprod.
42. Moses R.M., Stenhouse C., Halloran K.M., Sah N., Newton M.G., Hoskins E.C., Washburn S.E., Johnson G.A., Wu G. & Bazer F.W. (2024b) Metabolic pathways of glucose and fructose: II spatiotemporal expression of genes involved in synthesis and transport of lactate in ovine conceptuses. Biol Reprod.
43. Nava-Trujillo H. & Rivera R.M. (2023) Large offspring syndrome in ruminants: current status and prediction during pregnancy. Animal 17, 100740.
44. Newton M.G., Stenhouse C., Halloran K.M., Sah N., Moses R.M., He W., Wu G. & Bazer F.W. (2023) Regulation of synthesis of polyamines by progesterone, estradiol, and their receptors in uteri of cyclic ewesdagger. Biol Reprod 109, 309-18.
45. Ortega M.S., Lockhart K.N. & Spencer T.E. (2024) Impact of Sire on Embryo Development and Pregnancy. Vet Clin North Am Food Anim Pract 40, 131-40.
46. Plewes M.R., Przygrodzka E., Monaco C.F., Snider A.P., Keane J.A., Burns P.D., Wood J.R., Cupp A.S. & Davis J.S. (2023) Prostaglandin F2alpha regulates mitochondrial dynamics and mitophagy in the bovine corpus luteum. Life Sci Alliance 6.
47. Prezotto L.D., Keane J.A., Cupp A.S. & Thorson J.F. (2023) Fibroblast Growth Factor 21 Has a Diverse Role in Energetic and Reproductive Physiological Functions of Female Beef Cattle. Animals (Basel) 13.
48. Rio Feltrin I., Guimaraes da Silva A., Rocha C.C., Ferraz P.A., da Silva Rosa P.M., Martins T., Coelho da Silveira J., Oliveira M.L., Binelli M., Pugliesi G. & Membrive C.M.B. (2024) Effects of 17beta-estradiol on the uterine luteolytic cascade in bovine females at the end of diestrus. Theriogenology 213, 1-10.
49. Rishi J.K., Timme K., White H.E., Kerns K.C. & Keating A.F. (2023) Obesity partially potentiates dimethylbenz[a]anthracene-exposed ovotoxicity by altering the DNA damage repair response in micedagger. Biol Reprod 108, 694-707.
50. Rishi J.K., Timme K., White H.E., Kerns K.C. & Keating A.F. (2024a) Altered histone abundance as a mode of ovotoxicity during 7,12-dimethylbenz[a]anthracene exposure with additive influence of obesitydagger. Biol Reprod 110, 419-29.
51. Rishi J.K., Timme K., White H.E., Kerns K.C. & Keating A.F. (2024b) Trajectory of primordial follicle depletion is accelerated in obese mice in response to 7,12-dimethylbenz[a]anthracene exposure. Biol Reprod.
52. Rocha C.C., Martins T., Silva F.A.C.C., Sponchiado M., Pohler K.G. & Binelli M. (2023) Viperin (RSAD2) gene expression in peripheral blood mononuclear cells of pregnant crossbred beef cows is altered by Bos indicus genetics. Theriogenology 209, 226-33.
53. Sah N., Stenhouse C., Halloran K.M., Moses R.M., Seo H., Burghardt R.C., Johnson G.A., Wu G. & Bazer F.W. (2023) Creatine metabolism at the uterine-placental interface throughout gestation in sheepdagger. Biol Reprod 109, 107-18.
54. Seo H., Bazer F.W. & Johnson G.A. (2024) Early Syncytialization of the Ovine Placenta Revisited. Results Probl Cell Differ 71, 127-42.
55. Seo H., Melo G.D., Oliveira R.V., Franco-Johannsen G., Bazer F.W., Pohler K. & Johnson G. (2023) Immunohistochemical examination of the utero-placental interface of cows on days 21, 31, 40, and 67 of gestation. Reproduction 167.
56. Sharawy H.A., Hegab A.O., Mostagir A., Adlan F., Bazer F.W. & Elmetwally M.A. (2023) Expression of genes for transport of water and angiogenesis, as well as biochemical biomarkers in Holstein dairy cows during the ovsynch program. Theriogenology 208, 52-9.
57. Silva C.S., Vianna da Costa E.S.E., Nunes Dode M.A., Mendes Cunha A.T., Garcia W.R., Sampaio B.F.B., Borges Silva J.C., Vaz F.E.M., Kerns K., Sutovsky P. & Nogueira E. (2023a) Semen quality of Nellore and Angus bulls classified by fertility indices and relations with field fertility in fixed-time artificial insemination. Theriogenology 212, 148-56.
58. Silva F., Martins T., Sponchiado M., Rocha C.C., Pohler K.G., Penagaricano F. & Binelli M. (2023b) Hormonal profile prior to luteolysis modulates the uterine luminal transcriptome in the subsequent cycle in beef cross-bred cowsdagger. Biol Reprod 108, 922-35.
59. Silva F.A., Martins T., Sponchiado M., Rocha C.C., Ashrafi N., Graham S.F., Pohler K.G., Peñagaricano F., Gonella-Diaza A.M. & Binelli M. (2023c) Pre-estrus progesterone does not affect post-estrus luminal metabolome in cross-bred beef cows. Reproduction 166, 99-116.
60. Smith M.S., Hickman-Brown K.J., McAnally B.E., Oliveira Filho R.V., de Melo G.D., Pohler K.G. & Poole R.K. (2023) Reproductive microbiome and cytokine profiles associated with fertility outcomes of postpartum beef cows. J Anim Sci 101.
61. Snider A.P., Gomes R.S., Summers A.F., Tenley S.C., Abedal-Majed M.A., McFee R.M., Wood J.R., Davis J.S. & Cupp A.S. (2023) Identification of Lipids and Cytokines in Plasma and Follicular Fluid before and after Follicle-Stimulating Hormone Stimulation as Potential Markers for Follicular Maturation in Cattle. Animals (Basel) 13.
62. Stenhouse C., Bazer F.W. & Ashworth C.J. (2023a) Sexual dimorphism in placental development and function: Comparative physiology with an emphasis on the pig. Mol Reprod Dev 90, 684-96.
63. Stenhouse C., Halloran K.M., Newton M.G., Moses R.M., Sah N., Suva L.J., Gaddy D. & Bazer F.W. (2023b) Characterization of TNSALP expression, localization, and activity in ovine utero-placental tissuesdagger. Biol Reprod 109, 954-64.
64. Stenhouse C., Newton M.G., Halloran K.M., Moses R.M., Sah N., Suva L.J. & Bazer F.W. (2023c) Phosphate, calcium, and vitamin D signaling, transport, and metabolism in the endometria of cyclic ewes. J Anim Sci Biotechnol 14, 13.
65. Stevens A.V., Myers C.A., Hall J.B. & Chibisa G.E. (2024) The Effects of Harvest Maturity of Eragrostis tef ‘Moxie’Hay and Supplemental Energy Source on Forage Utilization in Beef Heifers. Animals 14, 254.
66. Sung E., Park W., Park J., Bazer F.W., Song G. & Lim W. (2024) Meptyldinocap induces implantation failure by forcing cell cycle arrest, mitochondrial dysfunction, and endoplasmic reticulum stress in porcine trophectoderm and endometrial luminal epithelial cells. Sci Total Environ 924, 171524.
67. Sutovsky P., Hamilton L.E., Zigo M., Ortiz D'Avila Assumpcao M.E., Jones A., Tirpak F., Agca Y., Kerns K. & Sutovsky M. (2024) Biomarker-based human and animal sperm phenotyping: the good, the bad and the ugly. Biol Reprod.
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