SAES-422 Multistate Research Activity Accomplishments Report

Sections

Status: Approved

Basic Information

Participants

Coleman, Jeff (jjcoleman@auburn.edu) – Auburn University; Richards, Jon (JRichards@agcenter.lsu.edu) – Louisiana State University – AgCenter; Antoniel, Everton Paschoal (evepan@dtu.dk) – Technical University of Denmark (DTU); Brown, Daren (daren.brown@ars.usda.gov) - USDA-ARS Peoria, Illinois; DeGenring, Liza (degen059@umn.edu) - USDA-ARS, St. Paul, Minnesota; Drott, Milton (Milton.drott@usda.gov) – USDA-ARS, Cereal Disease Lab, St. Paul, Minnesota; Eagan, Justin (jeagan@wisc.edu) – University of Wisconsin-Madison; Ebert, Malaika (malaika.ebert@ndsu.edu) – North Dakota State University; Elmore, Mitch (mitch.elmore@usda.gov) – USDA-ARS, Cereal Disease Lab, St. Paul, Minnesota; Forster, Heather (hdforester@ksu.edu) – Kansas State University; Friesen, Tim (Timothy.Friesen@ars.uada.gov) - USDA-ARS, Fargo, North Dakota; Gill, Upinder (upinder.gill@ndsu.edu) – North Dakota State University; Hatmaker, Anne (ehatmake@umn.edu) – USDA-ARS, St. Paul, Minnesota; Infate, Rubyhyn (rubyhyn.infante@ndsu.edu) – North Dakota State University; Kariyawasam, Gayan (gkariyaw@utk.edu) – University of Tennessee; Keller, Nancy (npkeller@wisc.edu) – University of Wisconsin-Madison; Kim, Da-woon (kimdawol@msu.edu) – Michigan State University; Liu, Zhaohui (zhu.liu@ndsu.edu ) – North Dakota State University; Lofton, Lily (lily.lofton@uga.edu) – USDA-ARS, Athens, Georgia; Luciano Rodario, Dianiris (dianirisluciano@gmail.com) – USDA-ARS; Malvestiti, Michele (Mike) – michele.malvestiti@usda.gov) – USDA-ARS, Fargo, North Dakota; Martinez Soto, Domingo (dmartinez@cicese.edu.mx) – CICESE; McVey, Aidan (asbmcvey@ksu.edu) – Kansas State University; Reubec, Lynne (lynne.reubec@enko.as) – ENKO; Richter Michael (mrichter4@huskers.unl.edu) – University of Nebraska-Lincoln; Rollins, Jeff (rollinsj@ufl.edu) - University of Florida; Seo, Hye-Won (hseo45@ wisc.edu) – University of Wisconsin-Madison; Todd, Richard (rbtodd@ksu.edu) - Kansas State University; Trail, Frances (trail@msu.edu) - Michigan State University; Wilson, Richard (rwilson10@unl.edu) – University of Nebraska-Lincoln; Wassano, Natalia Seywi (wassano@wisc.edu) – University of Wisconsin-Madison; Wise, Roger (rodger.wise@usda.gov) – USDA-ARS, Ames, Iowa; Xu, Jin-Rong (jinrong@purdue.edu) - Purdue University

NCCC-307 Meeting Minutes – March 14, 2024

7:05 pm – 7:08 pm – Welcome and opening comments by Jeff Coleman

7:08 am – 8:30 pm – Research presentations (~30 minutes each followed by brief period for questions)

  1. Justin Eagan, Keller Lab, University of Wisconsin-Madison. “The apple pathogen Penicillium expansum and its interactions with host microbiota”
  2. Michele Malvestiti, Friesen Lab, USDA-ARS, North Dakota State University. “Ducks in a row: multiple Pyrenophora teres teres effectors target a single host susceptibility locus in barley”
  3. Annie Hatmaker, Drott Lab, USDA-ARS, Cereal Disease Laboratory, University of Minnesota. “Pathogenicity is associated with population structure in a fungal pathogen of humans”

 8:30 pm – 8:40 pm – Break

 8:40 pm – 9:10 pm – Discussion

             There were two main points of discussion during the meeting: 1) the organization of the comprehensive review of known/characterized fungal effectors, and 2) the next annual meeting.

            One of the main points of discussion concerning the effector review was how to focus/limit the effectors that would be covered in the manuscript. Options discussed included: 1) all known effectors, 2) those with a known gene-for-gene interaction, or 3) effectors within a defined number of fungal pathogens or model systems. This discussion will continue through email in an effort to establish the scope of the review. Additionally, it was mentioned to encourage graduate students to partake in composing the manuscript.

            As for the next meeting, it was agreed upon to hold the next meeting during the fall of 2025, although the location was not determined.

Accomplishments

NCCC307 committee members collectively increased our understanding of biochemistry and genetics of plant-fungal interactions this past year by: 1) increasing our understanding of the fungal effectors and modulation of host plant immune response, 2) better understanding the function of mycotoxins and their regulation, 3) understanding of RNA editing in phytopathogenic fungi, and 4) increased understanding of plant resistance against fungal pathogens.  These efforts have produced more than 15 peer-reviewed journal publications from October 2023 through the March 2024 reporting period.

Impacts

  1. NCCC307 members aim to use their research findings to increased understanding of how plant-fungi interactions impact economically important diseases of crops and food production and threats to humans and animals through the consumption of mycotoxin contaminated foods.
  2. There are several ongoing collaborations between member’s research groups (Caplan and Vaillancourt; Friesen, Liu, Richards, and Gill; Keller and Drott). The collaboration between the Caplan and Vaillancourt laboratories was initiated after the last annual meeting and involves investigating the role of extracellular vesicles being released by Colletotrichum spp. during infection.
  3. A joint review article that will collect the details of characterized fungal effectors for submission to an appropriate journal. This topic was further refined during the discussion at the annual meeting (see summary of previous meeting above). Ideally over the next year this collaborative effort will yield a working draft of this compendium for fungal effectors.
  4. The research generated from members of this NCCC307 multistate project is of use to plant pathologists, plant breeders, and other scientists involved in the development of control strategies aimed at reducing crop diseases and mycotoxin contamination problems caused by fungi. The research will also benefit academic, government, and private-sector organizations that assess the risks that fungi pose to human and animal health.

Publications

  1. Aboukhaddour R, Hafez M, McDonald M, Moffat CS, Navathe S, Friesen TL, Strelkov SE, Oliver RP, Tan K-C, Liu ZH, Moolhuijzen PM, Phan H, See P-T, Solomon PS (2023) A revised nomenclature for ToxA haplotypes across multiple fungal species. Phytopathology 113:1180-1184.
  2. Bian ZY, Wang ZY, Wang DW, Xu JR (2024) Sexual stage-specific A-to-I mRNA editing is mediated by tRNA editing enzymes in fungi. Proceedings of the National Academy of Sciences USA  121: e2319235121. doi.org/10.1073/pnas.2319235121.
  3. Brabham HJ, De La Cruz DG, Were V, Shimizu M, Saitoh H, Hernandez-Pinzon I, Green P, Lorang J, Fujisaki K, Sato K, Molnar I, Simkova H, Dolezel J, Russell J, Taylor J, Smoker M, Gupta YK, Wolpert T, Talbot NJ, Terauchi R, Moscou MJ (2024) Barley MLA3 recognizes the host-specificity effector Pwl2 from Magnaporthe oryzae. Plant Cell 36: 447-470. org/10.1093/plcell/koad266.
  4. Ding MY, Cao SL, Xu D, Xia A, Wang Z, Wang W, Duan K, Wu C, Wang Q, Liang J, Wang D, Liu H, Xu JR, Jiang C (2023) A non-pheromone GPCR specifically regulates meiosis and ascosporogenesis via a conserved MAP kinase pathway in Fusarium graminearum. Proceedings of the National Academy of Sciences USA  120 (42): e2313034120. doi.org/10.1073/pnas.2313034120.
  5. Huang PP, Yu X, Liu H, Ding MY, Wang ZY, Xu JR, Jiang C (2024) Regulation of TRI5 expression and DON biosynthesis involves a lncRNA in Fusarium graminearum. Nature Communications 15: 1216. doi: 10.1038/s41467-024-45502-w.
  6. Li J, Wyatt NA, Skiba RM, Kariyawasam GK, Richards JK, Effertz K, Rehman S, Brueggeman RS, Friesen TL (2023) Pathogen genetics identifies avirulence/virulence loci associated with barley chromosome 6H resistance in the Pyrenophora teres teres – barley interaction. BioRxiv https://doi.org/10.1101/2023.02.10.527674
  7. Manan F, Shi G, Gong H, Hou H, Khan H, Leng Y, Castell-Miller C, Ali S, Faris JD, Zhong S, Steffenson B, Liu ZH (2023) Prevalence and importance of the necrotrophic effector gene ToxA in Bipolaris sorokiniana populations collected from spring wheat and barley. Plant Disease 107: 2424-2430.
  8. Otero M, Pokhrel A, Seo S, Wendell L, Luangkhot AS, Lawrence KS, Coleman JJ (2024) Evaluation of genetic diversity, haplotype, and virulence of Fusarium oxysporumsp. vasinfectum field isolates from Alabama. Phytopathology. doi.org/10.1094/PHYTO-11-23-0438-R.
  9. Nelson AC, Kariyawasam G, Wyatt NA, Li J, Haueisen J, Stukenbrock EH, Borowicz P, Liu Z, Friesen TL (2023) Unleashing the secrets of plant-fungal interactions using a transformation-free confocal staining technique that supports AI-assisted quantitative analysis. BioRxiv doi: https://doi.org/10.1101/2023.10.04.560942.
  10. Qi Z, Lu P, Long X, Cao X, Wu M, Xin K, Xue T, Gao X, Huang Y, Wang Q, Jiang C, Xu JR, Liu H. (2024) Adaptive advantages of restorative RNA editing in fungi for resolving survival-reproduction tradeoffs. Science Advances 10 (1): eadk6130. doi.org/10.1126/sciadv.adk6130.
  11. Richards JK, Li J, Koladia V, Wyatt NA, Rehman S, Brueggeman RS, Friesen TL (2024) A Moroccan population of Pyrenophora teres teres defeats the barley chromosome 6H broad-spectrum resistance. Phytopathology 114:193-199.
  12. Rodriguez-Herrera KD, Vargas A, Amie J, Price PP, Salgado LD, Doyle VP, Richards JK, Moseley D, Rojas A, Thomas-Sharma S (2024) Development of a greenhouse assay to screen soybean varieties for resistance to aerial blight caused by Rhizoctonia solani AG1-IA. Phytopathology org/10.1094/PHYTO-10-23-0390-KC
  13. Searight J, Famoso AN, Zhou X, Doyle VP, Richards JK (2023) A high-quality genome assembly for Cercospora janseana, causal agent of narrow brown leaf spot of rice. Molecular Plant-Microbe Interactions 36(10):666-669
  14. Wang YY, Li R, Wang DW, Qian B, Bian Z, Wei J, Wei XL, Xu JR (2023) Regulation of symbiotic interactions and primitive lichen differentiation by UMP1 MAP kinase in Umbilicaria muhlenbergii. Nature Communications. 14: article number 6972. www.nature.com/articles/s41467-023-42675-8.
  15. Xin KY, Zhang Y, Fan L, Qi ZM, Feng CJ, Xu JR, Liu HQ (2023) Experimental evidence for the functional importance and adaptive advantage of A-to-I RNA editing in fungi. Proceedings of the National Academy of Sciences USA 120 (12): e2219029120. doi.org/10.1073/pnas.221902912.
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