Brief Summary of Minutes of Annual Meeting

Brief Summary of Annual NC1173 Multi-State Project Meeting

Minutes were taken by Priya Basu (Mississippi State University)

• List of attendees in person: Priya Chakrabarti Basu, Margarita López-Uribe, Judy Wu-Smart, Kate Anton (for Christina Grozinger), Ana Heck, Robyn Underwood, Jamie Ellis, Declan Schroeder, David Wick, Cameron Jack, Lewis Bartlett, Brock Harpur, Juliana Rangel, David R. Tarpy, Zachary Huang, Reed Johnson, Esmaeil Amiri, Hongmei Li-Byarlay and Michelle Flenniken
• List of attendees via Zoom: Christine Hamilton, Jeffrey Harris, Brian Spiesman, Geoffrey Williams, Rachel Vannette, Hollis Woodard, Tania Kim, Ramesh Sagili and Rachael Winfree
• The meeting minutes: Margarita presided over the meeting as chair. She rotated off and vice-chair Priya is now the new Chair. Brock is the new vice-chair. NC1173 currently has 41 members from 24 states. With the project renewal, we currently have 31 members from 17 states.
• The director’s report gave a brief overview of NC1173 as one of the five regional associations. NC1173 focuses on multistate efforts for the project and not on individual efforts. All land grant universities receive hatch funds of which 25% must be spent for multistate activities. This year was the renewal year for NC1173. Margarita worked with NC1173 administrators and the members in submitting the project renewal. Brian already reviewed the proposal. The advisory review is still pending. By the end of March we will have a better idea about the revisions and have another three months for final submission and approval. The renewed proposal name will continue to be NC1173. The deadline to submit project report is January 29, 2024.
• Judy asked whether reporting by region rather than institution was possible or not. Margarita indicated it will be hard as we will need regional representatives. Margarita will create a working spreadsheet to catalog all participants’ grants and publications. A consensus was to use the AAPA website for hosting multistate effort outcomes.
• It was also suggested to alternate the NC1173 meeting location between ABRC and the International Pollinator Conference. So next year we will meet at Reno with ABF—the year after we will meet with the Internal Pollinator Conference in Montana.
• There is now a new objective 3 on bee monitoring. It was also suggested that each year of the project, there can be a focus on each objective of the renewed NC1173. Suggestions were also included about having a mid-year retreat or zoom call to catch up on the progress of the project between participants from various universities.

Objective 1a: (Biotic Stressors: Pests & Pathogens)

 Honey bees are attacked by a large number of parasites and pathogens. Varroa destructor—an ectoparasitic mite that feeds on honey bees—is one of the deadliest pests currently facing the US beekeeping industry. Varroa mites transmit viruses within and between colonies, including deformed wing virus (DWV), which are often associated with overwinter losses of honey bee colonies. In addition, managed honey bees are threatened by bacterial (e.g., American and European Foulbrood) and eukaryotic pathogens (e.g., Vairimorpha) and other pests (e.g., small hive beetles). Growing evidence has demonstrated that some of these honey bee pathogens can be picked up by wild bees visiting shared floral resources with honey bees. This potential for pathogen transmission has raised concerns about the consequences that poor pest and disease management in honey bees may have on wild bee fauna. 

Short-term Outcomes:  

• Based on research on the benefits of propolis to honey bee health, a bee supply company (Premiere Bee Products LLC) is now selling deep Langstroth-style boxes with rough, grooved interior walls that stimulate colonies to construct a propolis envelope. The propolis envelope provides stability to the immune system and microbiome and helps lower disease loads.
• Offering queen producers training on knowledge and improved techniques about diagnostic tools that have allowed them to produce and commercialize healthier queens.
• Demonstration that increasing floral diversity reduces pathogen loads in honey bees and wild bees.

Outputs:

• Experimental work demonstrating that the presence of honey bee colonies in soybean fields and prairies has no detectable impact on wild bee communities. Instead, wild bee communities are influenced by the composition of the surrounding landscape, with most species showing negative relationships with cropland cover (Toth, ISU).
• Discovery and characterization of pathogens in honey bees and wild bees (Ellis, UF; Hines, PSU; Rangel, TAMU).
• Characterization of the phenology of pests across different regions of the US. This information serves as a foundation for Varroa treatment models, aiding beekeepers in the future as a part of a holistic approach to control this pest (Jack, UF).
• Efficacy of treatments for nosemosis found that the available treatments failed to reduce spore prevalence and intensity in treated bees. These studies have identified an area in need of future research (Ellis and Jack, UF; Sagili R).
• Evidence that increasing floral diversity reduces pathogen loads in wild bees (Hines and Grozinger, PSU).

Activities:

• Team of scientists described honey bee infection with amoebic disease leading to an amplified effort to screen honey bees for this poorly described pathogen and conduct in vitro tests to determine its pathogenicity to honey bees (Ellis and Jack, UF).
• Investigations of the role of thymol as an immune stimulator that can reduce viral loads (Flenniken, MSU).  
• Investigations to characterize honey bee host-virus interactions through experiments that evaluated the impact of sublethal virus infections on honey bee health, using flight distance as a quantitative proxy for overall health (Flenniken, MSU). 
• Workshops and training opportunities for professional queen producers titled (Amiri, MSU).
• Ongoing research efforts to develop new chemical options for small hive beetle control (Bartlett, UGA).
• Continued collaborations with industry (Dalan Animal Health) to combine bee vaccines with supplemental feeding for honey bee pathogen control (Delaplane and Bartlett, UGA).
• Research on a new potential pest Tropilaelaps mite to better understand how they disperse within and between colonies (Williams, Auburn).
• Novel data on pathogen dynamics between managed and wild bees in agroecosystems indicating that pathogen spillover is highly context-dependent (López-Uribe, PSU).

Milestones:

• If our renewal project is approved, our goal is to publish shared protocols for pathogen quantification in honey bees and wild bees to increase opportunities for meta-analyses of data collected across the United States. This is the main goal for our shared multi-state efforts for year 2025.

Objective 1b: (Abiotic Stressors: Pesticides, Forage Availability, Nutrition)

Major abiotic stressors contributing to honey bee health decline include pesticide exposure, malnutrition, and climatic instability. NC1173 members are assessing the effects of these interacting factors on bees and their pollination services through laboratory assays, field experiments and landscape-level data. 

Short-term Outcomes:  

• Launched Penn State Honey and Pollen Diagnostics Lab which allows beekeepers, growers, researchers, and the public to submit samples for DNA metabarcoding analysis (Grozinger, PSU). 
• Training citizen scientists, 4H students, and USDA and USGS scientists on pollen hand collection and bee nutrition (Basu, MSU).

Outputs:

• Demonstrated antagonistic effects of neonicotinoid insecticides and ectoparasitic mites on Apis mellifera health (Williams, Auburn).
• Studies demonstrating that bumble bee species vary in heat resilience with queens being especially susceptible, and humidity levels need to be considered in the impacts of heat waves on bees (Hines, PSU). 
• Improved lists of optimal plants for bumble bees and our understanding of the macronutrient requirements across bees enable a predictive framework for selecting optimal foraging plants (Hines and Grozinger, PSU).
• Student training on data collection to understand how land management and climate change affect wild bee communities (Kim and Spiesman, KSU). 
• Development of a mobile app for land owners and the general public for identifying bee pollinators (Kim and Spiesman, KSU).
• Demonstrated that land use and weather conditions have led to a decline in honey production over the last 50 years, which facilitated the identification of ecoregions, land use, and weather conditions associated with improved nutrition and honey production (Grozinger, PSU). 

Activities:

• Development of AI-based classifiers for bee species identification of wild and museum species. These tools will accelerate wild bee research by overcoming the taxonomic bottleneck for this type of research (Kim and Spiesman, KSU).
• Comparison of the thermal physiology of queen bumble bees between a species experiencing local declines (Bombus auricomus) and a species exhibiting continent-wide increases (B. impatiens) found limited ability to acclimate to temperature (Hines, PSU). 
• Research project to test the effect of common insecticides on the reproductive quality of queens and their intergenerational effects. In addition, we investigated the effect of plastic queen cups on the physiology and growth of honey bee queens (Amiri, MSU).
• New studies on the effect of simultaneous exposure of bees to immune system stimulating thyme oil and the neonicotinoid insecticide, clothianidin (Fleinniken, MSU).
• Combination of DNA metabarcoding and biochemical methods to analyze the source and nutritional content of bee-collected pollen from diverse bee species (Grozinger, PSU).
• Investigations to determine how attractive nutritional supplements are to honey bees. Studies use caged worker bees with commercially available pollen substitutes (AP23, MegaBee, UltraBee) and wildflower pollen (Ellis and Jack, UF).
• Assessment of floral visitation in bumble bees across the season and years through DNA barcoding in pollen combined with macronutrient ratios of pollen were used to better understand optimal forage for supporting diverse bumble bee communities (Hines and Grozinger, PSU).
• Implementation of transcriptomic approaches to obtain molecular signatures of bee stressors across different landscapes (Hines and Grozinger, PSU).
• Investigated using a juvenile hormone analog to reduce varroa mite reproduction in worker brood (Huang, MSU).

Milestones:

• If our renewal project is approved, our goal is to develop a database of pollen lipids, proteins, phytosterols and amino acids that is publicly available for researchers, policymakers, citizens, and stakeholders. This is the main goal for our shared multi-state efforts for year 2028.

Objective 2: (Genetics, Breeding, & Diversity)

Breeding mite and disease resistant traits in honey bee stock and diversifying honey bee genetics and selection efforts are more sustainable solutions to address the pest and pathogen issues in honey bees and is a long-term goal for NC1173 members. 

 Short-term Outcomes:

• Breeding-focused training events with beekeepers. Most attendees report an increase in knowledge of basic breeding skills that is likely to change their management practice as a result of training (Harpur, Purdue).

 Outputs:

• Developed a new standardized test for AHB and a centralized testing facility. We have already sequenced 2,000 honey bees from 38 states using this platform (Harpur, Purdue; López-Uribe, PSU).
• Combined transcriptomics, genomics, and selective breeding using instrumental insemination for assisted breeding programs (Harpur, Purdue).
• Using controlled reciprocal crosses, genome sequencing, and transcriptomics, new data demonstrates that genes inherited from the mothers and fathers have differential effects on bee behavior and physiology (Grozinger, PSU).

 Activities:

• Active selection programs of breeds that resist the Varroa mites have resulted in over 100 colonies that have survived at least one winter without treatments (Spivak, UMN). 
• Testing whether colonies that express hygienic behavior show resistance to Deformed Wing Virus (Spivak, and Schroeder, UMN).

 Milestones:

• If our renewal project is approved, the main milestone for this objective is to review the available information on honey bee stock in the United States and their phenotypic traits. This information will be shared via a peer-reviewed publication and extension materials. This is the main goal for our shared multi-state efforts for year 2026. 

Objective 3: (Management)

 Management practices to maintain healthy honey bees and landscapes that support pollinators are in high demand and recommendations continue to evolve with new research. Therefore, NC1173 members strive to engage in research activities that are relevant to stakeholder needs to better provide the most up-to-date, science-based recommendations to beekeepers, pesticide applicators, farmers, homeowners, and policymakers. Efforts for this objective include recommendations on how to better manage pests and pathogens in honey bees, enhancing landscapes for pollinators, and options to reduce exposure or mitigate the effects of pesticides.  

Short-term Outcomes:  

• Major revision of the Beekeeping in Northern Climates short course manual and online course. This material is freely available online (Spivak, UMN).
• Education of beekeepers on how to induce a summer brood break as a tool for Varroa control (Bartlett, UGA).
• Expansion of Beescape decision support tool to help beekeepers, growers, conservationists, and the public manage landscapes to improve bee health (Grozinger, PSU).
• Learning of beekeeping basic techniques to control pests, 80% of participating beekeepers expressed that they intend to implement learned knowledge, and 58% thought the information they learned would save them more than $50 (Williams, Auburn).

 Outputs:

• Honey bees experience positive health effects (increased bee population and colony weight) when they live near prairie strips integrated into crop fields compared to crop fields without these landscape enhancements (Toth, ISU).
• Increased flower resources in natural habitats produced a nested network supporting higher bee richness and a greater chance for bee-mediated plant pollination. Some bee groups showed improved health indicators at prairie strip sites and these may be influenced by evolutionary family, foraging preferences, and body size (Toth, ISU). 
• Statistics reporting national losses and drivers of honey bee losses in the United States (Williams, Auburn).
• Cass et al. conducted surveys of farmers, landowners, and beekeepers and summarized the findings in an article recently accepted at the Journal of Integrated Pest Management. Overall, we document strong support and enthusiasm for pollinator-friendly practices in Iowa, but note gaps in knowledge about which practices are most effective and what tools are available to implement them. These results suggest a way for pollinator extension programs to address these knowledge gaps in a receptive group of beekeepers, farmers, and landowners.
• Hosted practical workshops with a focus on IPM tools for beekeeping. This was a coordinated online beekeeping webinar called “At Home Beekeeping” with participation from 12 institutions (Auburn, UF, UGA, U Tennessee, NCSU, KSU, MSU, LSU, TAMU, USDA-ARS, USDA-ARS Stoneville, USDA-ARS Baton Rouge, USDA-ARS Poplarville).
• Development of optimal organic beekeeping management practices for stationary beekeeping operations in the northeast (López-Uribe, PSU)
• Quarterly column in American Bee Journal demystifying misunderstandings about organic beekeeping management (López-Uribe, PSU)

 Activities:

• Coordinated efforts to assess winter capped brood monitoring (https://aub.ie/winterbrood) (UF, UGA, MSU, U Tennessee, TAMU, USDA-ARS, OSU, Cornell, Central State, PSU)
• Updated the Beescape decision support tool based on survey responses from beekeepers, growers, conservationists, and scientists. The fool now allows for more flexible analyses and provides the economic value of pollination services to crops (Grozinger, PSU).
• Development of unique beekeeping support systems for hobby and commercial beekeepers in MN and the North Central region (Spivak, UMN).
• Collected a large data set on the native bee species found in forests of different successional ages. In addition to the bee species, pollen collected from hundreds of bee specimens was also identified (Winfree, Rutgers).

Milestones:

• If our renewal project is approved, the main milestone for this objective is to two-fold. First, we aim to develop a centralized repository with documents about best management practices for honey bees and landscapes for different regions across the United States; and (2) make publicly available general recommendations for plant species (e.g., seed mixes) and their nutritional value to wild and managed bees. These are the main goals for our shared multi-state efforts for year 2029.

Summary table and list of publications by topic reported by NC1173 committee members for 2023. NC1173 authors are indicated in bold.

Objective 1a: Biotic Stressors (Pests & pathogens)

Clair ALS, Zhang G, Dolezal AG, O’Neal ME, Toth AL (2023) Agroecosystem landscape diversity shapes wild bee communities independent of managed honey bee presence Agriculture, Ecosystems & Environment 327, 107826

Dickey M, Whilden M, Twombly Ellis J, Rangel J (2023) A preliminary survey reveals that common viruses are found at low levels in a wild population of honey bees (Apis mellifera). Journal of Insect Science. 23(6): 26; 1–11.

Gratton EM, McNeil DJ, Grozinger CM, Hines HM. (2023) Local habitat type influences bumble bee pathogen loads and bee species distributions” Environmental Entomology 52 (3), 491-501 https://doi.org/10.1093/ee/nvad027

Iredale ME, Viadanna PHO, Subramaniam K, Tardif E, Bonning BC, Ellis JD. Report of amoebic disease in a colony of Western honey bees (Apis mellifera). Veterinary Pathology, 2023;60(5): 709-713. https://doi.org/10.1177/03009858231179956. 

Jack CJ, Oliveria IdB, Kimmel CB, Ellis JD. Seasonal differences in Varroa destructor population growth in western honey bee (Apis mellifera) colonies. Frontiers in Ecology and Evolution, 2023;11:1102457. https://doi.org/10.3389/fevo.2023.1102457. 

Liu J, Zhang R, Tang R, Zhang Y, Guo R, Xu G, Chen D, Huang ZY, Chen Y, Han R, Li, W. The role of honey bee derived aliphatic esters in the host-finding behavior of Varroa destructor. Insects 2023, 14, no. 1: 24. https://doi.org/10.3390/insects14010024

Orlova M., Porter M, Hines HM, Amsalem E. Symptomatic infection with Vairimorpha bombi decreases diapause survival in wild bumble bee species (Bombus griseocollis). Animals 2023;13 (10):1656

Powell JE, Lau P, Rangel J, Arnott R, DeJong T, Moran NA (2023) The microbiome and gene expression of honey bee workers are affected by a diet containing pollen substitutes. PLoS ONE. 18(5): e0286070. https://doi.org/10.1371/journal.pone.0286070 

Prouty C, Jack C, Sagili R, Ellis JD. Evaluating the efficacy of common treatments for Vairimorpha (Nosema) spp. control. Applied Sciences, 2023;13(3): 1303. https://doi.org/10.3390/app13031303.

Ray AM, Gordon EC, Seeley TD, Rasgon JL, Grozinger CM. (2023) Signatures of adaptive decreased virulence of deformed wing virus in an isolated population of wild honey bees (Apis mellifera). Proceedings of the Royal Society B 290(2009). https://doi.org/10.1098/rspb.2023.196. 

Wen P, Chen J, Huang ZY. (2023). Death recognition by undertaker honey bees based on reduced cuticular hydrocarbon emissions. Entomologia Gereralis. DOI: 10.1127/entomologia/2023/1607

Objective 1b: Abiotic Stressors (Pesticides, nutrition, landscapes)

Bruckner S, Straub L, Neumann P, Williams GR. (2023) Negative but antagonistic effects of neonicotinoid insecticides and ectoparasitic mites Varroa destructor on Apis mellifera honey bee food glands. Chemosphere 313:137535. https://doi.org/10.1016/j.chemosphere.2022.137535

Chakrabarti P, Sagili RR. Managed foraging for honey and crop pollination – Honey bees as livestock. Chapter 8 in the book The Foraging Behavior of the Honey Bee (Apis mellifera L.). 2023:175–193. Edited by John Purdy, Elsevier Academic Press, ON, Canada. 

Crone M, Boyle N, Bresnahan S, Biddinger D, Richardson R, Grozinger CM. (2023) More than mesolectic: Characterizing the nutrition niche of Osmia cornifrons” Ecology and Evolution 13(10), e10640. https://doi.org/10.1002/ece3.10640.

Feuerborn, C., Quinlan, G., Shippee, R., Strausser, T.L., Terranova, T., Grozinger CM, Hines HM. (2023) Variance in heat tolerance in bumble bees correlates with species geographic range and is associated with several environmental and biological factors” Ecology and Evolution 13, e10730. https://doi.org/10.1002/ece3.10730. 

Lau P, Sgolastra F, Williams GR, Straub L. (2023) Editorial: Insect pollinators in the Anthropocene: How multiple environmental stressors are shaping pollinator health. Front Ecol Evol. 2023;11:1279774 https://doi.org/10.3389/fevo.2023.1279774.

Lau PW, Esquivel IL, Parys KA, Hung K-LJ, Chakrabarti P. (2023) The nutritional landscape in agroecosystems: a review on how resources and management practices can shape pollinator health in agricultural environments. Annals of the Entomological Society of America:1-15.

Manaswi A, Noordyke E, Prouty C, Ellis JD. (2023) Western honey bee (Apis mellifera L.) attraction to commercial pollen substitutes and wildflower pollen in vitro. Journal of Applied Research 147: 244-247. http://dx.doi.org/10.1111/jen.13102. 

Quinlan GM, Miller DAW, Grozinger CM (2023) Examining spatial and temporal drivers of pollinator nutritional resources: Evidence from five decades of honey bee colony productivity data. Environmental Research Letters 18(11): 114018 DOI 10.1088/1748-9326/acff0c 

Quinlan GM, Grozinger CM (2023) Honey bee nutritional ecology: From physiology to landscapes. Advances in Insect Physiology https://doi.org/10.1016/bs.aiip.2023.01.003. 

Quinlan GM, Feuerborn C, Hines HM, Grozinger CM (2023)Beat the heat: Thermal respites and access to food associated with increased bumble bee heat tolerance Journal of Experimental Biology 226 (17): jeb245924. doi: https://doi.org/10.1242/jeb.245924. 

Objective 2: Genetics, Breeding, Diversity

Bresnahan ST, Lee E, Clark L, Ma R, Rangel J, Grozinger CM, Li-Byarlay H (2023) Examining parent-of-origin effects on transcription and RNA methylation in mediating aggressive behavior in honey bees (Apis mellifera). BMC Genomics. https://doi.org/10.1186/s12864-023-09411-4

Cambron-Kopco L, Underwood RM, Given JK, Harpur BA, López-Uribe MM. (2023) Honey bee stocks exhibit high levels of intra-colony variation in viral loads. J. Apic. Res. 1–4 doi:10.1080/00218839.2023.2285153 

Gmel AI, Guichard M, Dainat B, Williams GR, Eynard S, Vignal A, Servin B, the Beestrong Consortium, and Neuditschko M. Identification of runs of homozygosity in western honey bees (Apis mellifera) using whole-genome sequencing data. Ecol Evol. 2023;13:e9723. https://doi.org/10.1002/ece3.9723

Karlikow M, Amalfitano E, Yang X, Doucet J, Chapman A, Mousavi PS, Homme P, Sutyrina, P, Chan W, Lemak S, Yakunin, AF, Dolezal AG, Kelley S, Foster LJ, Harpur BA, Pardee K. CRISPR-induced DNA reorganization for multiplexed nucleic acid detection. Nature Communications. 2023; doi:10.1038/s41467-023-36874-6 

Morfin N, Harpur BA, De la Mora A, Guzman-Novoa E. Breeding honey bees (Apis mellifera L.) for low and high Varroa destructor population growth: gene expression of bees performing grooming behavior. Frontiers in Insect Science. 2023; doi:10.3389/finsc.2023.951447

Objective 3: Management

 Abou-Shaara HF, Jack CJ, Ellis JD. The impact of smoke and fogged/vaporized thyme oil on honey bee (Apis mellifera) survival and behavior in vitro. Journal of Apicultural Research, 2023;62(3): 643-645. https://doi.org/10.1080/00218839.2022.2135809.

Bartlett LJ, Baker C, Bruckner S, Delaplane KS, Hackmeyer EJ, Phankaew C, Williams GR, Berry JA. (2023) No evidence to support the use of glycerol–oxalic acid mixtures delivered via paper towel for controlling Varroa destructor (Mesostigmata: Varroidae) mites in the Southeast United States. Journal of Insect Science 23(6):18.

Berry JA, Braman SK, Delaplane KS, Bartlett LJ. Inducing a summer brood break increases the efficacy of oxalic acid vaporization for Varroa destructor (Mesostigmata: Varroidae) control in Apis mellifera (Hymenoptera: Apidae) colonies. Journal of Insect Science. 2023 Nov 1;23(6):14.

Borchardt KE, Kadelka C, Schulte LA, Toth AL (2023) An ecological networks approach reveals restored native vegetation benefits wild bees in agroecosystems. Biological Conservation 287, 110300.

Browning AD,  Smitley D,  Studyvin J, Runkle ES, Huang ZY,  Hotchkiss E (2023). Variation in pollinator visitation among garden cultivars of marigold, portulaca, and bidens. Journal of Economic Entomology 116: 872–881

Cass RP, Hodgson EW, O’Neal ME, Toth AL, Dolezal AG (2023) Attitudes about honey bees and pollinator-friendly practices: a survey of Iowan beekeepers, farmers, and landowners. Journal of Integrated Pest Management 13 (1), 30. 

Cruz SM, Grozinger CM. (2023). Mapping student understanding of bees: Implications for pollinator conservation. Conservation Science and Practice, 5( 3), e12902. https://doi.org/10.1111/csp2.12902 (2023).

Hackmeyer EJ, Washburn TJ, Delaplane KS, Bartlett LJ. (2023) Successful application of anthranilic diamides in preventing small hive beetle (Coleoptera: Nitidulidae) infestation in honey bee (Hymenoptera: Apidae) colonies. Journal of Insect Science 23(6):12.

Genung MA,  Reilly J, Williams NM, Buderi A, Gardner J, Winfree R (2023) Rare and declining bee species are key to consistent pollination of wildflowers and crops across large spatial scales. Ecology 104: e3899 https://doi.org/10.1002/ecy.3899

Petitta IR, López-Uribe MM, Sabo AE. (2023) Biology and management of wild lupine (Lupinus perennis L.): a case study for conserving rare plants in edge habitat. Plant Ecology  [link]

Prestby TJ, Robinson AC, McLaughlin D, Dudas PM, Grozinger CM. (2023)“Characterizing user needs for Beescape: A spatial decision support tool focused on pollinator health” Journal of Environmental Management 325: 116416 (2023). https://doi.org/10.1016/j.jenvman.2022.116416

Prouty C, Abou-Shaara HF, Stanford B, Ellis JD, Jack C. (2023) Oxalic acid application method and treatment intervals for reduction of Varroa destructor populations in western honey bee (Apis mellifera) colonies. Journal of Insect Science. 23(6): 13. 

Prouty C, Jack C, Sagili R, Ellis JD. (2023) Evaluating the efficacy of common treatments used for Vairimorpha (Nosema) spp. Control. Applied Sciences. 13(3): 1303.

Underwood RM, Lawrence B, Turley NE, Cambron-Kopco L, Kietzman P, Traver BE, López-Uribe MM. (2023) A longitudinal experiment demonstrates that organic beekeeping management systems support healthy and productive honey bee colonies. Scientific Reports 13(1): 6072

Weinman LR, Ress T, Gardner J, Winfree R. (2023). Individual bee foragers are less efficient transporters of pollen for the plants from which they collect the most pollen into their scopae. American Journal of Botany DOI: 10.1002/ajb2.16178 

Zhang G, Murray CJ, Clair AL, Cass RP, Dolezal AG, LA Schulte, O’Neal ME, Toth AL (2023) Native vegetation embedded in landscapes dominated by corn and soybean improves honey bee health and productivity. Journal of Applied Ecology 60 (6), 1032-1043

Other peer-reviewed publications

Bresnahan ST, Galbraith D, Ma R, Anton K, Rangel J, Grozinger CM (2023) Beyond conflict: Kinship theory of intragenomic conflict predicts individual variation in altruistic behaviour. Molecular Ecology. https://doi.org/10.1111/mec.17145 

Freitas F, Branstetter M, Franceschini-Santos V, Dorchin A, Wright K, López-Uribe MM, Griswold T, Silveira F, Almeida E. (2023). UCE phylogenomics, biogeography, and classification of long-horned bees (Hymenoptera: Apidae: Euceerini), with insights on using specimens with extremely degraded DNA. Insect Systematics and Diversity 7(4):3

Gutierrez GM, LeCroy LA, Roulston TH, Biddinger DJ, López-Uribe MM. (2023) Osmia taurus (Hymenoptera: Megachilidae): A new non-native bee species with invasiveness potential in North America. Environmental Entomology 52(2):149–156

Han B, Amiri E, Wei Q, Tarpy DR, Strand MK, Xu S, Rueppell O. Group size influences maternal provisioning and compensatory larval growth in honeybees. Iscience. 2023 Dec 15;26(12).

Miles GP, Liu XF, Amiri E, Grodowitz MJ, Allen ML, Chen J. Co-Occurrence of wing deformity and impaired mobility of alates with Deformed Wing Virus in Solenopsis invicta Buren (Hymenoptera: Formicidae). Insects. 2023 Sep 27;14(10):788.

Mortensen AN, Ellis JD. (2023) Honey bees reared in isolation adhere to normal age-related division of labor when introduced into a colony. Applied Animal Behavior Science 258: 105824. https://doi.org/10.1016/j.applanim.2022.105824.

Pope N, Singh A, Childers A, Kapheim K, Evans J, López-Uribe MM. (2023) Expansion of agriculture drives adaptive evolution in a specialized squash pollinator. PNAS 120(15): e2208116120

Prouty C, Bartlett LJ, Krischik V, Altizer S. Adult monarch butterflies show high tolerance to neonicotinoid insecticides. Ecological Entomology. 2023 Apr 13.

Rangel J, Lau P, Strauss B, Hildinger E, Hernandez B, Rodriguez S, Bryant V, Tarone AM (2023) A Texas population of blow flies (Diptera: Calliphoridae) highlights underappreciated aspects of their biology. Ecological Entomology. 1–10. https://doi.org/10.1111/een.13298 

Sandoval-Arango S, Branstetter M, Cardoso C, López-Uribe MM. (2023). Phylogenomics reveals within species diversification but incongruence with color phenotypes in widespread orchid bees (Hymenoptera: Apidae: Euglossini). Insect Systematics and Diversity 7(2):1-13

Non peer-reveiwed publications

Anton K, Darnell C, Grozinger CM, Underwood R. “An Introduction to Honey Bee Breeding Program Design”. Penn State Extension. 2023 https://extension.psu.edu/an-introduction-to-honey-bee-breeding-program-design https://pollinators.psu.edu/news/honey-bees-may-inherit-altruistic-behavior-from-their-moth

Bruckner S, López-Uribe MM, Underwood RM (2023) Organic and Treatment-free Colony Management – They are not the same. American Bee Journal 163(10):1123-1125.

Chakrabarti, P. Young Zoologist - Honey Bees. neon Squid Books, McMillan Publishers, London.

Delaplane K, Hudson W, Johnson A. Yellow-Legged Hornet. UGA Extension bees.caes.uga.edu

Dickey M*, Rangel J (2023) Comparative quantification of honey bee (Apis mellifera) associated viruses in wild and managed colonies. In: López-Uribe MM, Chakrabarti P, Harpur BA (eds.) Proceedings of the 2023 American Bee Research Conference. Bee Culture. A https://doi.org/10.55406/ABRC.23

Lee K, Reuter GS, Spivak M. Beekeeping in Northern Climates. Third Edition. University of Minnesota Extension. 2024 https://drive.google.com/file/d/1VCZSD89XPYtWmNn3vh9IypfGpNUMYics/view

López-Uribe MM, Chakrabarti P, Harpur BA, Rangel J, Goblirsch M, Underwood R (2023) Introduction to the Proceedings. In: López-Uribe MM, Chakrabarti,P., Harpur, BA (eds.) Proceedings of the 2023 American Bee Research Conference. Bee Culture. A https://doi.org/10.55406/ABRC.23

López-Uribe MM, Underwood RM, Bruckner S (2023) The benefits of organic beekeeping and how management affects honey bee colony health, survival, and productivity. American Bee Journal 163(7):753-755.

Rangel J, Fei C, Chen Y, Woodward R (2023) Market implications of changes in climate, land coverage and annual colony loss rates for U.S. commercial beekeeping operations. In: López-Uribe MM, Chakrabarti P, Harpur BA (eds.) Proceedings of the 2023 American Bee Research Conference. Bee Culture. A https://doi.org/10.55406/ABRC.23 

Rangel J, Pino M (2023) Food 4 Farmers: A nonprofit organization working with coffee growers in Latin America on beekeeping and alternative farming activities to overcome food insecurity during the “thin” months. American Bee Journal. 163(11): 1227-1230. 

Smith D, Rangel J, Bouga M, Parejo M (2022 and 2023) Special issue on stingless bees. Journal of Apicultural Research. 61(5): 577-577, https://doi.org/10.1080/00218839.2022.2122307 

Toth, A. 2023. Honey bees: Good guys or bad guys? Beeline (Newsletter of the Central Iowa Beekeepers' Association), Spring 2023 Issue.

Twombly Ellis J*, Rangel J (2023) Pesticide stress drives premature self-removal behavior in honey bee (Apis mellifera) workers. In: López-Uribe MM, Chakrabarti,P., Harpur, BA (eds.) Proceedings of the 2023 American Bee Research Conference. Bee Culture. A https://doi.org/10.55406/ABRC.23 [25] 

Vu AT, Ellis JD. 2023. Episode 130: Controlling Varroa Through Genetic Technology. Two Bees in a Podcast. https://podcasters.spotify.com/pod/show/ufhbrel 

Vu, A.T., Ellis, J.D. 2023. Episode 127: Varroa Control Methods in Ontario. Two Bees in a Podcast. https://podcasters.spotify.com/pod/show/ufhbrel

Vu, A.T., Ellis, J.D. 2023. Episode 131: Follow-up on Microplastics. Two Bees in a Podcast. https://podcasters.spotify.com/pod/show/ufhbrel 

Vu AT, Ellis JD. 2023. Episode 135: Shipping Queen Cells. Two Bees in a Podcast. https://podcasters.spotify.com/pod/show/ufhbrel 

Vu AT, Ellis JD. 2023. Episode 147: Generational Beekeeping and Queen Breeding with Ted Miksa. Two Bees in a Podcast. https://podcasters.spotify.com/pod/show/ufhbrel 

Vu AT, Ellis JD. 2023. Episode 149: Drones Galore! Two Bees in a Podcast. https://podcasters.spotify.com/pod/show/ufhbrel