Brief Summary of Minutes of Annual Meeting

January 24, 2023: 27 members in attendance, 7 online

8:00 A.M. Introduction by Katelyn Kesheimer

8:10 A.M. Administrative update by John Ruberson

- Project rewrite submitted by September 2024; final proposal due December 1, 2024

- Excellence in Multistate Research Award due February 28. Award is $15,000 ($5K for travel; $10K to be used towards project)

- Next meeting tentatively Jan 23-25^th, 2024

 Individual state reports

• Alabama: low overall pest pressure - high temp, high drought early
• Florida: armigera, no detections. Resistance monitoring, Bt and insecticides. Resistance to diamide in Spodoptera exigua
• Georgia: 10-15% increase in acreage. Stink bugs number one pest; high numbers early, shut down by hot weather. Older Cry traits failed completely for CEW, Cry2 traits with some suppression (20-30%), only VIP performing well
• Illinois: CRW population update. Bt bioassays on SmartStax Pro for NCR and WCR, NCR very susceptible, some survival by WCR in Champaign. Populations very resistant to Cry3 traits
• Indiana: no/few issues with NCRW. very low numbers of WCR, some growers moving away from below-ground traits. no issues with FAW in 2022. study comparing robot vs human scouting, low defoliation compromised study.
• Iowa: NCR & WCR, fairly high pressure around the state; Numerous projects on CRW. Identified virus integrations in SCRW Work on impact of habitat manipulation on monarch butterfly populations. Good CEW pressure in sweet corn sentinel monitoring, VIP still providing full control, other traits compromised. Working with a resistant FAW population on genetic control.
• Kansas: Looking at EPN efficacy in western KS. CEW one of bigger pests. Field in eastern Kansas 25% infested with ECB. Isolated issues with CRW; some chinch bug in corn.
• Kentucky: Low insect pressure. Brown marmorated stink bugs, started to see in soybean and corn since 2020. Lots of Japanese beetle silk feeding. No issues with FAW in 2022. Some non-GMO acreage with ECB.
• Louisiana: No change in resistance status of CEW, still very resistant to Cry1/Cry2 traits. Low populations of FAW in 2022.
• Maryland: network of pheromone traps, testing different pheromones, cost/benefit of seed treatments study. Llot of use of Capture in-furrow on top of seed treatments, looking at impact of these practices on slug predation/populations.
• Michigan: Asiatic garden beetle, variable stunting of plants due to AGB. Redesign of Bt trait table, draft is available, up to 1.5 pages, available for review.
• Minnesota: traits not working well for WCRW. expansion of NCRW range, lot of work needed. no yield difference in insecticide on top of Bt traits. ECB areawide suppression study - random survey (~85% Bt). Using non-Bt fields to understand effect of "halo" effect of Bt corn planting. ECB hot spots where farmers have multiple years of non-Bt corn/silage corn.

 10 A.M. Sara Delheimer, USDA-NIFA, multi-state impact statements

• Stress change in knowledge, behavior, or condition
• Use concise, accessible language free from jargon
• Recent NC246 impact statements were generally considered strong

 11:55 A.M. Special announcement by Tom Hunt:

• Opportunity to submit a symposium to international convention of plant protection, July 1-5 2024 in Athens, Greece

 1:30 P.M. State reports continued:

• Missouri: looking for postdoc, will soon hire a USDA Research Entomologist.
• Nebraska: high WBC numbers in state, including areas with historically lower numbers. Late plantings acting as trap crops for CRW, CEW and others. Dry overall, spider mites in corn. Japanese beetle interest (new to area). Popcorn growers have issues with ECB from time to time, frequent insecticide applications. Moderate to very high CRW problems in irrigated areas. Three-year project evaluating current CRW tactics. Reduction in NIR with insecticides but typically not a yield loss. Pyramids compromised, typically don't lodge.
• North Carolina: stink bugs main insect pest; numbers low in 2022. Stink bug issues at ear formation. New project on overwintering of stink bugs near corn. Southern corn billbug is a major issue on a limited acreage (poor drainage mostly), seems to be expanding. Some VIP corn injury - limited geography, drought pressure; increase in RRs this year.
• Ohio: Monitoring FAW, CEW, and WBC in 2022. Slug trapping study in corn, better sampling with pitfalls than shingle traps. Asiatic garden beetle.
• Ontario: WBC, similar situation to previous years. Lot of resistance cases with CRW. ECB, Cry1F- resistant populations found in Montreal and one in Manitoba, New Brunswick, Nova Scotia. Strongly recessive resistance trait, no fitness cost. Almost 400 trap sites in CRW monitoring network, new system for data entry. Working on documenting impact of CRW on forage quality. Potential regulatory issues with lambda-cyhalothrin in feed. Dimethoate-resistant spider mites becoming a problem.
• South Dakota: relatively high CRW pressure. low CEW moth flights. research on ground beetle populations, soil invertebrate communities. CRW colonies will be downsized.
• Tennessee: using Cry1F-resistant FAW as a model to implement genetic marker-based resistance monitoring. examining resistance mechanisms to Bt in FAW and CEW. CEW typically binding site alteration, FAW seems to be modifications to proteolysis. currently producing Bt proteins for resistance monitoring bioassays,
• Texas: Some CEW. No signs of VIP3 resistance (Corpus Christi). Not seeing resistance issues with fall armyworm. Hired new extension entomologist in Amarillo. Continuing sweet corn Bt sentinel plots; comparison study with Bt field corn (ABSTC-recommended screen). Lubbock: extremely high FAW flights in May and June.
• Wisconsin: No FAW; CRW resistance concerns. Slug problems in northern Wisconsin.
• Delaware: slugs and stink bugs were most important pests. Spider mite activity in seedling corn in some cases.

 3:30 P.M., Brad Coates: The western corn rootworm genome and beyond

• CRW genome
• Genes for chemosensory are expanded compared with other beetles, particularly monophagous beetles
• ATP binding casettes and CP450s (involved in pesticide resistance) also expanded
• Coates et al. 2023 (perhaps 2022)

4:00 P.M. Christian Krupke: Next steps for the Bt hybrids: Trait costs vs. rootworm damage

• Trait use outpaces actual need
• How to put the economic analysis of this information to use for stakeholders?

 4:30 P.M. Elson Shields: The interaction of neonic seed treatments and persistent EPNs along with seed corn maggot vs seed treatments in NY

• Observed interaction between EPNs and insecticide seed treatments for CRW control
• https://persistentbiocontrol.com

January 25, 2023 (Open session with industry representatives): 30 in attendance, 7 online (estimated)

 8:00 A.M. Introductions by Chair (Katelyn Kesheimer)

 8:15 A.M. Juan Luis Jurat-Fuentes: Targeted sequencing as a tool to improve resistance screening

• Amplification and detection of resistance alleles. FAW is model
• Working on methods to detect unknown resistance mechanisms
• Will try to apply to ECB which has same/similar mechanism for Cry1F resistance.

 8:30 A.M. Emily Bick: Digital entomology

• Using lidar-based sensors to detect insects
• Tractor mounted sensors to detect insect density in real time. Using machine learning to classify species.
• Future uses: automated phenotyping for insect resistance; precision insect mapping; quantifying biodiversity in different settings; regional insect monitoring/forecasting
• Working on cheaper sensors

 9:00 A.M. Kara Welch (US EPA): EPA update

• Lepidopteran IRM framework currently under development
• Will probably be finished this year
• New IRM staff on board

 9:30 A.M. Galen Dively: Sweet corn monitoring network

• Using sweet corn as a diagnostic screen to detect Bt field susceptibility changes.
• Compares Bt varieties with true isolines
• Cry1Ab PFR has gradually increased over time (=1.0) Cry2Ab2 PFR around 0.92-0.95.

 10:05 A.M. Special announcement by Tom Sappington:

• 2023 International Working Group on Ostrinia meeting in Kenya

 10:15 A.M. Matt Carroll (Bayer CropScience): status of IRM framework for lepidopteran pests of Bt corn and cotton, academic research licenses

• Update on IRM framework: establishing corn sentinel plot network in cotton states.
• Sweet corn preferred, but field corn is an option. Focused on VIP3A.
• Targeting late-normal planting date. Goal is to implement resistance mitigation efforts in cotton based on results.
• Establishing WBC network as well.
• E-mail Matt Carroll to sign up for sentinel plot network.

 11:00 A.M. Group discussion on relaxed research agreements

• Situation is largely similar to last few years

 1:30 P.M. Katelyn Kesheimer/Chris DiFonzo: Lepidoptera in hemp

• Main pests are CEW, ECB, WBC.
• Feeding assays of WBC on hemp indicate some survival. In hemp, top three caterpillar pests are CEW, yellowstriped armyworm, FAW.
• Current work on IPM strategies for H. zea, greenhouse pest management, impacts of N fertilizer/irrigation on arthropod populations. Fire ants also a major issue

 2:00 P.M. Pat Porter: Corn earworm adult emergence

• Moth emergence period from corn is narrow.
• Manipulated irrigation
• Non-irrigated corn dramatically reduced adult emergence

 2:30 P.M. Kelley Tilmon: Slugs

• Looking at relationship between cover crops and slugs, impact of insecticides on slug populations in corn-soy rotations, degree day modeling, threshold establishment, nematodes as a slug biocontrol.

 2:35 P.M. Introduction of Kelsey Fisher (Connecticut Agriculture Experiment Station)

• Integrating pollinator management with pest management.
• Insect movement and dispersal ecology, works with stable isotopes. Interested in discussing CRW dispersal, studying using stable isotopes.

 2:45 P.M. Fei Yang, Texas A&M (starting at Minnesota in May)

• Bt resistance monitoring in cotton, Cry1Ac, Cry2Ab, and VIP3A. 137 field populations.
• Dose-response diet overlay bioassay. No VIP RR > 10. RR gradually increasing, small overall RR.
• Found synergistic effects of above-ground toxins on CEW.

 4:00 P.M. Dominic Reisig: Bt refuge compliance

• Multiple factors: common pool resource, limited marketing of refuge hybrids, conducting surveys on availability of non-Bt seeds.
• Emotional appeal more effective than information alone in proportion of respondents who intended to plant a refuge.

January 26, 2023: 17 individuals in attendance

 8:00 A.M. Updates from Local Arrangements committee

• Motion to accept Savannah with Cleveland as a secondary option; motion carries

 8:05 A.M. Nominations committee

• Silvana Paula-Moraes was nominated and elected Secretary

 8:15 A.M. Tom Sappington: Resident and migrant phenotypes in western corn rootworm populations

• 10 years since last update on CRW dispersal behavior
• Argues WCRW is partially migratory species
• Discussion about biology and dispersal; Gus may have added some discussion about pheromone titers

 8:45 A.M. Adrian Pekarcik: Asiatic garden beetle research in Ohio

• Higher damage in sandy soils
• Golf cup cutter better sampling method
• Adult sampling via WBC trapping method (milk jug with propylene glycol)
• 1 grub/cup cutter sample results in stunting; 2 grubs/cup leads to plant death
• Low mortality (~30%) from a single year study of EPNs isolated from Ohio

 9:30 A.M. Chris Philips: NIFA updates

• Restaffing NIFA (80 to 300)
• Increase of about 74 million for NIFA
• 10 million dollar increase for AFRI over FY22
• Updates on CPPM, AFRI, Small Business, BRAG, and other grant programs
• Farm Bill to go in September 2023

 10 A.M. Adrian Pekarcik: Availability of western and northern corn rootworm from the USDA ARS lab in Brookings

• Most lines are going to be ended; some are going to be sent to other labs for maintenance
• Non-diapausing/diapausing WCR and non-diapausing NCR will be maintained

 10:30 A.M. Ashley Tessnow: Fall armyworm

• Texas haplotype origin for most of the USA
• Mixing of Florida and Texas genotypes in Alabama through New Jersey; Florida dominant in South Carolina and Florida
• Majority of genetic variation comes by strains; some geographic disparity within the corn strain
• Allochronic separation of strains – corn strains active earlier than rice strain

Individual state reports were received from 13 states (Delaware, Florida,  Iowa, Louisiana, Maryland, MIchigan, Missouri, Nebraska, North Carolina, North Dakota, South Carolina, Tennessee, and Texas), one Canadian province (Ontario) and one governmental agency (USDA-ARS). Listed in these reports are advancements related to corn pest management through many techniques and approaches.

Selected Outputs:

WCR Genome. The USDA-ARS unit in Iowa recently published the WCR genome. This published genome will allow for investigations into insecticide and Bt resistance.

Handy Bt Trait Table. This highly used document is maintained and updated by Dr. Chris DiFonzo with input from NC246 members. The Handy Bt Trait Table continues to be used by crop consultants, corn growers, agroindustry, academics, and government personnel to understand insect traits, efficacy, and product names.

Selected Extension Materials:

Reisig, D. D. and A. S. Huseth. 2022. Insect control in field corn. 2022 North Carolina Agricultural Chemicals Manual. North Carolina Cooperative Extension Service, College of Agriculture and Life Sciences, N.C. State University, Raleigh, N.C. pp. 76-79.

Reinders, J. D. 2022. Integrated Crop Management Conference FieldNotes: Western corn rootworm resistance management: A Nebraska perspective. Iowa State University Extension. Available from: https://www.aep.iastate.edu/fieldnotes/notes/145.pdf

Wright, R. J., W. Ohnesorg & J. McMechan. 2022.  Insect Management, pp. 309-348, in 2022 Guide for Weed, Disease, and Insect Management in Nebraska, EC 130, University of Nebraska-Lincoln Extension.

Robert Wright, Kyle Koch & Justin McMechan. 2022. Scout Emerging Corn for Insects; Don’t Assume Protection. https://cropwatch.unl.edu/2020/scout-emerging-corn-insects-dont-assume-protection

Robert Wright. 2022. Managing stalk borers in corn. https://cropwatch.unl.edu/common-stalk-borer-scouting

Gracie Keiter, Jeffrey Cluever, Julie Peterson, Robert Wright & Jeff Bradshaw 2022. Degree-days for Prediction of Western Bean Cutworm Flight. https://cropwatch.unl.edu/2021/degree-days-prediction-western-bean-cutworm-flight

Robert Wright, Justin McMechan & Kyle Koch. 2022. Japanese Beetles Emerging; Scout Corn and Soybean Fields.  https://cropwatch.unl.edu/2019/japanese-beetles

Julie Peterson, Robert Wright, Jeff Bradshaw & Thomas Hunt. 2022.  Scouting and Treatment Recommendations for Western Bean Cutworm. https://cropwatch.unl.edu/2020/scouting-and-treatment-recommendations-western-bean-cutworm

Robert Wright & Justin McMechan. 2022. Grasshoppers Return ― It’s Time to Scout Field Borders https://cropwatch.unl.edu/2019/grasshopper-management

Robert Wright & Julie Peterson. 2022. Scout Now for Corn Rootworm Beetles to Assess Potential Risk of Future Damage. https://cropwatch.unl.edu/2017/scout-now-corn-rootworm-beetles-assess-potential-risk-future-damage

Robert Wright, Julie Peterson & Tom Hunt. 2022. Identifying Spider Mite Damage and the Species Responsible. https://cropwatch.unl.edu/2016/identifying-spider-mite-damage-species-responsible

Robert Wright, Julie Peterson & Tom Hunt. 2022. Managing Spider Mites in Corn and Soybean. https://cropwatch.unl.edu/2016/managing-spider-mites-corn-and-soybean

Igwe, P.-G., Cramer, M. , Owens, D., Dively, G., and Hamby, K. Accepted. Managing slugs in field crops using IPM principals, University of Maryland Extension Fact Sheet

Cramer, M.E., and Hamby, K.A. 2022. Optimizing early season insect pest management in field corn. Maryland Grain Producers and Utilization Board 2022 Report.

Reisig, D., Kesheimer, K., Bateman, N., Studebaker, G., Meyer, R., Reay-Jones, F., Shields, E., Owens, D., Buntin, G.D., Seiter, N., Hodgson, E., Sisson, A., Zukoff, A., Villanueva, R., Towles, T., Hamby, K.,  DiFonzo, C., Hutchison, B., Potter, B., Catchot, A., Cook, D., Bradshaw, J., Peterson, J., Beauzay, P., Knodel, J., Tilmon, K., Baute, T., Varenhorst, A., Brown, S. Kerns, D., Porter, P., Taylor, S., and Jensen, B. 2022. Corn invertebrate loss estimates from the United States and Ontario, Canada - 2021. Crop Protection Network. CPN-2019-21 https://cropprotectionnetwork.org/publications/corn-invertebrate-loss-estimates-from-the-united-states-and-ontario-canada-2021

Brown, K., Brown, S., J.A. David, R. Diaz, B. Fitzpatrick, K. Healy, F. Huang, N. Lord, T. Reagan, D. Ring, M. Stout, T. Smith, Q. Sun, B. Wilson 2022. Louisiana Insect Pest Management Guide. LSU AgCenter. Pub. 1838. pp 233.

NDSU Extension Crop & Pest Report (newsletter): New website listed below. https://www.ndsu.edu/agriculture/ag-hub/ag-topics/crop-production/crop-pest-report

Multi-state Efforts:

• Pat Porter (Texas A&M AgriLife) and David Buntin (University of Georgia) continued to evaluate Bt resistance monitoring techniques with ABSTC support
• Bruce HIbbard (USDA-ARS) and Dalton Ludwick (Texas A&M AgriLife) continued to investigate Mexican corn rootworm biology
• Tracey Baute (Ontario Ministry of Agriculture, Food and Rural Affairs) organized the Great Lakes and Maritimes Pest Monitoring Network with five states and six Canadian provinces
• Juan Luis Jurat-Fuentes (University of Tennessee-Knoxville) produced and disseminated VIP3Aa39 protein for resistance monitoring efforts
• Dominic Reisig (North Carolina State University) and Sally Taylor (Virginia Polytechnic Institute and State University) received funding (USDA-NIFA-CPPM) to improve Extension efforts related to Bt corn refuge planting
• Lance Meinke (University of Nebraska-Lincoln) and Joseph Spencer (Illinois Natural History Survey) served as guest editors for a special issue in Insects entitled, “Corn Rootworm: Biology, Ecology, Behavior, and Integrated Pest Management”
• Erin Hodgson and Ashley Dean (Iowa State) continue to coordinate a multi-state corn rootworm trapping network to document changes in western and northern corn rootworm abundance in the U.S. and Canada

Impacts:

• NC246 members continued to have thousands of contacts through traditional Extension avenues.
• NC246 members in Nebraska, Illinois, and Iowa initiated projects to evaluate pest management techniques for corn rootworms across a broad geographic region
• Collaborative efforts to increase knowledge on pest biology were made across the globe, including in North America, Africa, and Asia
• Bt resistance monitoring with sweet corn sentinel plots, under the leadership of Galen Dively (University of Maryland), continued with 24 states and four Canadian provinces participating

Banerjee, R., C. P. de Bortoli, F. Huang, K. H. Lamour, R. Meagher, D. Buntin, X. Ni, F. P. Reay Jones, S. D. Stewart, and J. L. Jurat-Fuentes (2022).  Large genomic deletion linked to field-evolved resistance to Cry1F corn in fall armyworm (Spodoptera frugiperda) from Florida.  Scientific Reports, 12(1):13580 doi: 10.1038/s41598-022-17603-3.

Prabu, S., J. Dapeng, J. L. Jurat-Fuentes, Z. Wang, and K. He (2022).  Hemocyte response to treatment of susceptible and resistant Asian corn borer (Ostrinia furnacalis) larvae with Cry1F toxin from Bacillus thuringiensis.  Frontiers in Immunology, vol 13: pp.1022445.

Couch, T., T. Jackson, and J. L. Jurat-Fuentes.  2022.  Commercial production of entomopathogenic bacteria.  Mass Production of Beneficial Organisms (2nd Edition), Editors: Juan Morales-Ramos, M. Guadalupe Rojas, David Shapiro-Ilan. Chapter 12, pp. 359-373. Academic Press.

Arends, B., D. Reisig, S. Gundry, J. Greene, G. Kennedy, F. Reay-Jones, and A. Huseth. 2022. Helicoverpa zea (Lepidoptera: Noctuidae) feeding incidence and survival on Bt maize in relation to maize in the landscape. Pest Manage. Sci. 78: 2309-2315. doi: 10.1002/ps.6855

Reisig, D. D., C. DiFonzo, G. Dively, Y. Farhan, J. Gore, and J. Smith. 2022. Best management practices to delay the evolution of Bt resistance in lepidopteran pests without high susceptibility to Bt toxins in North America. J. Econ. Entomol. 115: 26-36. doi: 10.1093/jee/toab247

Van den Berg, J., D. Reisig, and M. Brewer. 2022. A special collection: Spodoptera frugiperda (fall armyworm): ecology and management of its world-scale invasion outside the Americas. J. Econ. Entomol. 115: 1725-1728 doi: 10.1093/jee/toac143

Reinders, J. D., E. E. Reinders, E. A. Robinson, W. J. Moar, P. A. Price, G. P. Head, and L. J. Meinke. 2022. Characterizing the sublethal effects of SmartStax® PRO dietary exposure on life history traits of the western corn rootworm, Diabrotica virgifera virgifera LeConte. PLOS ONE 17(5): e0268902. doi: 10.1371/journal.pone.0268902

Blessing Ademokoya, Kacie Athey & John Ruberson. 2022. Natural Enemies and Biological Control of Stink Bugs (Hemiptera: Heteroptera) in North America. Insects, 13(10), 932; https://doi.org/10.3390/insects13100932

Douglas Lawton, Anders Huseth, George Kennedy, Amy Morey, William Hutchison, Dominic Reisig, Seth Dorman, DeShae Dillard, Robert Venette, Russell Groves, John Adamczyk, Izailda Barbosa Dos Santos, Tracey Baute, Eric Burkness, Ashley Dean, Galen Dively, Seth Dorman, Helene Doughty, Shelby Fleischer, Jessica Green, Jeremy Greene, Krista Hamilton, Erin Hodgson, Thomas Hunt, Sean Malone, Fred Musser, David Owens, John Palumbo, Silvana Paula-Moraes, Julie Peterson, Francis Reay-Jones, Dominic Reisig, Silvia Rondon, Abby Seaman, Lori Spears, Scott Stewart, Sally Taylor, Tyler Towles, Celeste Welty, Joanne Whalen, Robert Wright, and Marion Zuefle. 2022. Helicoverpa zea population dynamics are driven by a continental overwintering gradient. PNAS.  DOI: 10.1073/pnas.2203230119

Coates, B. S., K. O. Walden, D. Lata, N. N. Vellichirammal, R. F. Mitchell, M. N. Anderson, R. McKay, M. D. Lorenzen, N. Grubs, Y. Wang, J. Han, J. L. Xuan, P. Willadsen, H. Wang, B. W.  French, R. Bansal, S. Sedky, D. Souza, D. Bunn, L. J. Meinke, N. J. Miller, B. D. Siegfried, T. W. Sappington, H. M. Robertson, 2023. A draft Diabrotica virgifera virgifera genome: insights into control and host plant adaptation by a major maize pest insect. BMC Genomics (2023) 24:19.  https://doi.org/10.1186/s12864-022-08990-y.

Carmona, G., E. Robinson, A. Rosa, C. Proctor, and A.J. McMechan. 2022. Impact of cover crop planting and termination dates on arthropod activity in the follow corn. Journal of Economic Entomology. 115 (4): 1177-1190.

Carmona, G. E. Robinson, J. Campos, and A.J. McMechan. 2022. Impact of timing and use of an insecticide on arthropods in cover crop-corn systems. Insects. 13(4): 348-366.

Krueger A.J., Rault L.C., Robinson E.A., Weissling T.J., Vélez A.M., and T.D. Anderson. 2022. Pyrethroid insecticide and milkweed cardenolide interactions on detoxification enzyme activity and expression in monarch caterpillars. Pesticide Biochemistry and Physiology. 187: 105173.

Rodrigues T.B., Rieske L.K., Narva K.E., Roberts A., and A.M. Vélez. 2022. Editorial: New Applications of Insecticidal RNAi. Frontiers in Agronomy. Frontiers in Agronomy. 4:903841.

Darlington M., Reinders J.D., Sethi A., Lu A.L., Ramaseshadri P., Fischer J.R., Boeckman C.J., Petrick J.S., Roper J.M., Narva, K.E., and A.M. Vélez. 2022. RNAi for western corn rootworm: Lessons learner, challenges, and future directions. Insects. 13(1): 57.

Reinders, J. D., and L. J. Meinke. 2022. Reduced susceptibility of western corn rootworm (Diabrotica virgifera virgifera LeConte) populations to Cry34/35Ab1-expressing maize in northeast Nebraska. Scientific Reports 12: 19221. doi: 10.1038/s41598-022-23755-z.

Reinders, J. D., E. E. Reinders, E. A. Robinson, B. W. French, and L. J. Meinke. 2022. Evidence of western corn rootworm (Diabrotica virgifera virgifera LeConte) field-evolved resistance to Cry3Bb1 + Cry34/35Ab1 maize in Nebraska. Pest Management Science 78(4): 1356-1366. doi: 10.1002/ps.6752.

Vélez A.M., Jurat-Fuentes J.L., Bohnenblust E., Rathore K., Smagghe G., Whyard S., Kogel K.H., and K.E. Narva. RNA Interference in Agriculture: Methods, Applications, and Governance. Council for Agricultural Science and Technology (CAST). Accepted.

DiFonzo, C.D. 2022. Western bean cutworm feeding and development on industrial hemp in the laboratory and field. Great Lakes Entomol. 55 (1): 1-9.

Reisig, D., K. Kesheimer, N. Bateman, G. Studebaker, R. Meyer, F. Reay-Jones, E. Shields, D. Owens, G. D. Buntin, N. Seiter, E. Hodgson, A. Sisson, A. Zukoff, R. Villanueva, T. Towles, K. Hamby, C. DiFonzo, B. Hutchison, B. Potter, A. Catchot, D. Cook, J. Bradshaw, J. Peterson, P. Beauzay, J. Knodel, K. Tilmon, T. Baute, A. Varenhorst, S. Brown, D. Kerns, P. Porter, S. Taylor, and B. Jensen. 2022. Corn invertebrate loss estimates from the United States and Ontario, Canada - 2021. Crop Protection Network. CPN-2019-21. DOI: https://doi.org/10.31274/cpn-20220722-0

Reisig, D., C. DiFonzo, G. Dively, J. Gore, Y. Farhan, J. Gore, and J. Smith. 2022. Best management practices to delay the evolution of Bt resistance in Lepidopteran pests without high susceptibility to Bt toxins in North America. Journal of Integrated Pest Management 115(1): 10-25.

Fritz, M.L. 2022. Utility and challenges of using whole-genome resequencing to detect emerging insect and mite resistance in agroecosystems. Evolutionary Applications 15(10): 1505-1520 DOI: 10.1111/eva.13484

Lawton, D., Huseth, A.S., Kennedy, G.G., Morey, A.C., Hutchison, W.D., Reisig, D.D., Dorman, S.J., Dillard, D., Venette, R.C., Groves, R.L., Adamczyk, J.J., Barbosa Dos Santos, I., Baute, T., Brown, S., Burkness, E., Dean, A., Dively, G.P., Doughty, H.B., Fleischer, S.J., Green, J., Greene, J.K., Hamilton, K., Hodgson, E., Hunt, T., Kerns, D., Leonard, B.R., Malone, S., Musser, F., Owens, D., Palumbo, J.C., Paula-Moraes, S., Peterson, J.A., Ramirez, R., Rondon, S.I., Schilder, T.L., Seaman, A., Spears, L., Stewart, S.D., Taylor, S., Towles, T., Welty, C., Whalen, J., Wright, R., Zuefle, M., 2022. Pest population dynamics are related to a continental overwintering gradient. Proc. Natl. Acad. Sci. U.S.A. 119, e2203230119. https://doi.org/10.1073/pnas.2203230119

Blanco, C.A., K. Conover, G. Hernandez, G. Valentini, M. Portilla, C.A. Abel, P. Williams, U. Nava-Camberos, W.D. Hutchison, and G.P. Dively. 2022. Grain Yield is not Impacted by early defoliation of maize: Implications for fall armyworm action thresholds. Southwestern Entomol.  47(2): 335-344.

Dively, G.P., and Terrence Patton. 2022. An evaluation of cultural and chemical control practices to reduce slug damage in no-till corn, Insects 13, no. 3: 277. https://doi.org/10.3390/insects13030277

Dominic D Reisig, Chris DiFonzo, Galen Dively, Yasmine Farhan, Jeff Gore, and Jocelyn Smith. 2022.  Best management practices to delay the evolution of Bt resistance in lepidopteran pests without high susceptibility to bt toxins in North America, J. Econ. Entomol. 115: 26-36,.

Krishnan, H.B., S. Kim, A.E. Pereira, A. Jurkevich, and B.E. Hibbard. 2022. Adenanthera pavonina, a potential plant-based protein resource: seed protein composition and immunohistochemical localization of trypsin inhibitors. Food Chemistry: X 13 (2022) 100253. https://doi.org/10.1016/j.fochx.2022.100253.

Paddock, K.J., D.L. Finke, K.S. Kim, T.W. Sappington, and B.E. Hibbard. 2022. Patterns of microbiome composition vary across spatial scales in a specialist insect.  Frontiers in Microbiol. 13:898744. https://doi.org/10.3389/fmicb.2022.898744

Huynh, M.P., B.E. Hibbard, K.-V. Ho, and K.S. Shelby. 2022. Toxicometabolomic profiling of resistant and susceptible western corn rootworm larvae feeding on Bt maize seedlings. Scientific Reports 12:11639. https://doi.org/10.1038/s41598-022-15895-z

Pereira, A.E., M.P. Huynh, K.J. Paddock, J.L. Ramirez, E.P. Caragata, G. Dimopoulos, H.B. Krishnan, S.K. Schneider, K.S. Shelby, and B.E. Hibbard. 2022. Chromobacterium Csp-P biopesticide is highly toxic to larvae of three Diabrotica species including strains resistant to Bacillus thuringiensis. Scientific Reports 12:17858. https://doi.org/10.1038/s41598-022-22229-6.

Pereira, A.E., R.W. Geisert, and Bruce E. Hibbard. 2022. Maize inbred Mp708 is highly susceptible to western corn rootworm, Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae), in field and greenhouse assays. J. Insect Sci. 22(6): 8; 1–5, https://doi.org/10.1093/jisesa/ieac067

Paddock, K.J., K. Dellamano, B.E. Hibbard, K.S. Shelby. eCry3.1Ab-resistant western corn rootworm larval midgut epithelia respond minimally to Bt intoxication. 2023. J. Econ. Entomol. (Published on-line without final page numbers)

Jabeur, R., V. Guyon, S. Toth, A.E. Pereira, M.P. Huynh, Z. Selmani, W. Paul, M. Bosio, L. Beuf, P. Clark, D. Vallenet, W. Achouak, C. Audiffrin, F. Torney, T. Heulin, B.E. Hibbard, S. Toepfer, and C. Sallaud. 2023. A novel binary pesticidal protein from Chryseobacterium arthrosphaerae controls Diabrotica virgifera virgifera via a different mode of action to existing commercial pesticidal proteins.  PLoS ONE: – accepted, in press. 

Lawton, D. et al. 2022. Pest population dynamics are related to a continental overwintering gradient. PNAS 119 (37) e2203230119.

Quan, Y., C. E. Mason, K. He, Z. Wang, and H. Wei. 2022. Impact of heat waves on egg survival and biological performance across life stages in the Asian corn borer. Entomol. Exp. Appl. 171: 129-137

Dillard, D., D. D. Reisig, and F.P.F. Reay-Jones. Helicoverpa zea (Lepidoptera: Noctuidae) in-season and overwintering pupation response to soil type. Environmental Entomology. In press.

Banerjee, R., C.P. de Bortoli, F. Huang, K. Lamour, R. Meagher, D. Buntin, X. Ni, F.P.F. Reay-Jones. S. Stewart, J.L. Jurat-Fuentes. 2022. Large genomic deletion linked to field-evolved resistance to Cry1F corn in fall armyworm (Spodoptera frugiperda) from Florida. Scientific Reports. 12: 13580.

Smith, J., and Y. Farhan. Monitoring resistance of Ostrinia nubilalis (Lepidoptera: Crambidae) in Canada to Cry toxins produced by Bt corn following detection of field-evolved resistance to Cry1Fa. (2022) Journal of Economic Entomology. In Press.

Farhan, Y., Limay-Rios, V., Schaafsma, A.W., and J.L. Smith. Susceptibility and field exposure of Striacosta albicosta (Lepidoptera: Noctuidae) eggs and larvae in Ontario, Canada to four insecticides. (2022) Pest Management Science. doi.org/10.1002/ps.6998.

Abel, C. A. and W. P. Williams.  2022. Evaluation of twenty maize germplasms from Belize, French Guiana, Guyana, and Suriname for resistance to leaf-feeding Spodoptera frugiperda. Southwestern Entomologist. 47:559-564.

Coates, B. S., K. K. O. Walden, D. Lata, N. N. Vellichirammal, R. F. Mitchell, M. N. Andersson, R. McKay, M. D. Lorenzen, N. Grubbs, Y. H. Wang, J. Han, J. L. Xuan, P., Willadsen, H. Wang, B. W. French, R. Bansal, S. Sedky, D. Souza, D. Bunn, L. J. Meinke, N. J. Miller, B. D. Siegfried, T. W. Sappington, H. M. Robertson, 2023. A draft Diabrotica virgifera virgifera genome: insights into control and host plant adaption by a major maize pest insect. BMC Genomics 24: 19. https://doi.org/10.1186/s12864-022-08990-y

Kurneth, H. D., S. M. Bogdanowicz, J. B. Searle, R. G. Harrison, B. S. Coates, G. M. Kozak, and E. B. Dopman. 2022. Consequences of coupled barriers to gene flow for the build-up of genomic differentiation. Evolution 76(5): 985-1002. https://doi.org/10.1111/evo.14466

Lata, D., B. S. Coates, K. K. O. Walden, H. M. Robertson, and N. J. Miller. 2021. Genome size evolution in the beetle genus Diabrotica. G3 12(4): jkac052. https://doi.org/10.1093/g3journal/jkac052

Paddock, K. J., D. L. Finke, K. S. Kim, T. W. Sappington, and B. E. Hibbard. 2022. Patterns of microbiome composition vary across spatial scales in a specialist insect. Frontiers in Microbiology 13: 898744.  https://doi.org/10.3389/fmicb.2022.898744

Stahlke, A. R., J. Chang, L. R. Tembrock, S. B. Sim, S. Chudalayandi, S. M. Geib, B. R. Scheffler, O. P. Perera, T. M. Gilligan, A. K. Childers, K. J. Hackett, and B. S. Coates. 2022. A chromosome-scale genome assembly of a Helicoverpa zea strain resistant to Bacillus thuringiensis Cry1Ac insecticidal protein. Genome Biol. Evol. https://doi.org/10.1093/gbe/evac131

Tong, D., L. Zhang, N. Wu, D. Xie, G. Fang, B. S. Coates, T. W. Sappington, Y. Liu, Y. Cheng, J. Xia, X. Jiang, and S. Zhan. 2022. The oriental armyworm genome yields insights into the long-distance migration of noctuid moths. Cell Reports 41(12): 111843. https://doi.org/10.1016/j.celrep.2022.111843

Blanco, C. A., K. Conover, G. Hernandez, G. Valentini, M. Portilla, C. A. Abel, P. Williams, U. Nava-Camberos, W. D. Hutchison, and G. P. Dively. 2022. Grain yield is not impacted by early corn defoliation: Implications for fall armyworm action thresholds. Southwestern Entomologist. 47:335-344.

Feng, M., Y. Zhang, B. S. Coates, Q. Du, Y. Gao, L. Li, H. Yuan, W. Sun, X. Chang, S. Zhou, and Y. Wang. 2023. Assessment of Beauveria bassiana for the biological control of corn borer, Ostrinia furnacalis, in sweet maize by irrigation application. BioControl https://doi.org/10.1007/s10526-022-10175-1

Grant, T. J., K. E. Fisher, N. Krishnan, A. N. Mullins, R. L. Hellmich, T. W. Sappington, J. S. Adelman, J. R. Coats, R. G. Hartzler, J. M. Pleasants, and S. P. Bradbury. 2022. Monarch butterfly ecology, behavior, and vulnerabilities in North Central United States agricultural landscapes. BioScience 72: 1176-1203. https://doi.org/10.1093/biosci/biac094

Liu, S., T. W. Sappington, B. S. Coates, and B. C. Bonning. 2022. Sequences encoding a novel toursvirus identified from southern and northern corn rootworms (Coleoptera: Chrysomelidae). Viruses 14(2): 397.  https://doi.org/10.3390/v14020397

Valmorbida, I., B. S. Coates, E. W. Hodgson, M. Ryan, and M. E. O’Neal. 2022. Evidence of enhanced reproductive performance and lack‐of‐fitness costs among soybean aphids, Aphis glycines, with varying levels of pyrethroid resistance. Pest Management Science, 78(5): 2000-2010. https://doi.org/10.1002/ps.6820

Wu, F., L. Zhang, Y. Liu, Y. Cheng, J. Su, T. W. Sappington, and X. Jiang. 2022. Population development, fecundity, and flight of Spodoptera frugiperda (Lepidoptera: Noctuidae) reared on three green manure crops: implications for an ecologically based pest management approach in China. J. Econ. Entomol. 115: 124–132.  https://doi.org/10.1093/jee/toab235

Tessnow, A.E., T.J. Raszick, P. Porter, and G.A. Sword. Patterns of genomic and allochronic strain divergence in the fall armyworm, Spodoptera frugiperda (J.E. Smith). 2022. Ecology and Evolution. 12(3): 1-14. doi: 10.1002/ece3.8706.

Kenis, M and 56 others (including F. Huang). 2022. Invasiveness, biology, ecology, and management of the fall armyworm, Spodoptera frugiperda. Entomol. Generalis, https://10.1127/entomologia/2022/1659.

de Oliveira, W.S., C.I.R Sakuno, L.L. Miraldo, M.A.G.C. Tavares, K.M.A., Komada, D. Teresani, J.L.X. Santos, and F. Huang. 2022.Varied frequencies of resistance alleles to Cry1Ab and Cry1Ac among Brazilian populations of the sugarcane borer, Diatraea saccharalis (F.). Pest Manag. Sci. 78: 5150–5163.

Banerjee, R., C.P. de Bortoli, F. Huang, K. Lamour, R. Meagher, D. Buntin, X. Ni, F. Reay-Jones, S. Stewart, and J.L. Jurat-Fuentes. 2022. Large genomic deletion linked to field-evolved resistance to Cry1F corn in fall armyworm (Spodoptera frugiperda) from Florida. Sci. Rep. 12:13580.

Lin, S., I. Oyediran, Y. Niu, S. Brown, D. Cook, X. Ni, Y. Zhang, F. P.F. Reay-Jones, J. S. Chen, Z. Wen, M. Dimase, and F. Huang. 2022. Resistance allele frequency to Cry1Ab and Vip3Aa20 in Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) in Louisiana and three other southeastern U.S. states. Toxins 4, 270. https://doi.org/10.3390/toxins14040270.

Yu, W., G. Head, and F. Huang. 2022. Inheritance of resistance to Cry1A.105 in Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae). Insects. 13, 875. https://doi.org/10.3390/insects13100875

Yu, W., G.P. Head, P. Price, S. Brown, D. Cook, X. Ni, F. P.F. Reay-Jones, M. Dimase, and F. Huang. 2022. Estimation of resistance allele frequencies to Cry1A.105 and Cry2Ab2 in the corn earworm (Lepidoptera: Noctuidae) with F2 isolines generated from a mass-mating method, Crop Prot. doi: https://doi.org/10.1016/j.cropro.2022.106054.

Rabelo, M.M., Dimase, M., Paula-Moraes, S.V. 2022. Ecology and management of the invasive land snail Bulimulus bonariensis (Stylommatophora: Bulimulidae) in row crops. Front. Insect Sci. https://doi.org/10.3389/finsc.2022.1056545

Flores-Rivera, X. L.G, Paula-Moraes, S.V., Johnson, J.W., Perera, O.P. 2022. Helicoverpa genus on the edge of the continental U.S.: Flight phenology, analysis of hybrid presence, and insecticide performance in high-input field crops in Puerto Rico. Front. Insect Sci. https://doi.org/10.3389/finsc.2022.1010310

Lawton D., Huseth. A.S., Kennedy,  G.G., Morey, A.C., Hutchison,  W.D., Reisig,  D.D., Dorman, S.J., Dillard, D., Venette, R.C., Groves, R.L., Adamczyk, J.J., Barbosa Dos Santos, I.G, Baute, T., Brown, S., Burkness, E., Dean, A., Dively, G.P., Doughty, H.B., Fleischer, S.J., Green, J., Greene, J.K., Hamilton, K., Hodgson, E., Hunt, T., Kerns, D., Leonard, B.R., Malone, S., Musser, F., Owens, D., Palumbo, J.C., Paula-Moraes, S., Peterson, J.A., Ramirez, R., Rondon, S.I., Schilder, T.L., Seaman, A., Spears, L., Stewart, S., Taylor, S., Towles, T., Welty, C., Whalen, J., Wright, R., Zuefle, M. 2022. Pest dynamics are driven by a continental overwintering gradient. Proceedings of the National Academy of Sciences, 119.

Resende, E.S., Beuzelin, J.M., Dunkley, V.E., Paula-Moraes, S.V., Seal, D.R., Nuessly, G.S. 2022. Pyrethroid susceptibility in field populations of picture-winged flies (Diptera: Ulidiidae) infesting fresh market sweet corn in Florida. Journal of Economic Entomology,115: 1685–1692. https://doi.org/10.1093/jee/toac12510. https://doi.org/1073/pnas.2203230119.