SAES-422 Multistate Research Activity Accomplishments Report
Sections
Status: Approved
Basic Information
- Project No. and Title: NE1834 : Genetic Bases for Resistance and Immunity to Avian Diseases
- Period Covered: 10/01/2020 to 09/30/2021
- Date of Report: 11/18/2021
- Annual Meeting Dates: 09/18/2021 to 09/19/2021
Participants
Robert Taylor, West Virginia University; Christopher Ashwell, West Virginia University; Gisela Erf, University of Arkansas; Ramesh Selvaraj, University of Georgia; Yvonne Drechsler, Western University; Lisa Bielke, Ohio State University; Ryan Arsenault, University of Delaware; Mark Parcells, University of Delaware; Calvin Keeler, University of Delaware; Matthew Koci, North Carolina State University; Keith Jaronsinski, University of Illinois; Janet Fulton, Hy-Line; Rami Dalloul, University of Georgia; Rodrigo Gallardo, UC Davis; Jiuzhou Song, University of Maryland; Shawna Weimer, University of Maryland; Susan Lamont, Iowa State University; Paul Cotter, Cotter Laboratory; Andrew Broadbent, University of Maryland; Mostafa Ghanem, University of Maryland, Ali Nazmi, Ohio State University; Billy Hargis, University of Arkansas. Students-Staff-Post doctoral scholars: Audrey Duff, Ohio State University (presented Station Report, Dr. Bielke’s lab); Brandi Sparling, PhD student, Western University (presented Station Report, Dr. Drechsler’s lab); Sofia Egaña, UC Davis (presented part of the Station Report, Dr. Gallardo’s lab); Theros Ng, Western University (Dr. Drechsler’s lab).
Accomplishments
Erf
Objective 2. Evaluation of the local (GF-pulp) cellular- and systemic (blood)-antibody responses to autogenous Salmonella vaccines (SVs) revealed that heterophils and macrophages dominated both the local primary and secondary responses to pulp injection of SVs, with only minor participation of lymphocytes. These inflammatory leukocytes persisted longer during the recall than the primary response. The lymphocyte recruitment in response to vaccine vehicle was not observed when mixed with Salmonella bacteria or LPS. Pulp injection of SVs initiated T-dependent antibody responses as indicated by isotype switch from IgM to IgG, and a faster and higher increase in plasma IgG levels following the second compared to the first immunization.
Objective 3. AR maintained and reproduced genetic lines that spontaneously develop autoimmune diseases. AR refined and expanded the use of the growing feather as an in vivo test-tube system to study innate and adaptive immune responses in poultry.
Arsenault
Objective 2. We elucidated a potential mechanism of why we observe differing responses to cocci challenge in older genetic lines as compared to new. The difference centers on the HIF1 pathway and involves apoptosis and glycolysis metabolism shifts, increasing in ACRB with a challenge dose. With collaborators in Germany, we reported on the first model of a chicken methylation biological clock that reflects the inflammatory state of the bird. Finally, we elucidated the mechanism of action of two distinct and important potential antibiotic alternative feed additives. These mechanism of action results may inform rational feed regimes to prevent performance loss due to management or challenge issues.
Koci
Objective 3. Our team at NCSU has worked to develop a new multiplex assay to allow for the assessment of gene expression levels of 50 targets, simultaneously, without the need to produce cDNA or perform RT-PCR. This system is based on the QuantiGene platform. This system uses a mixture of Luminex beads capable of producing 50 unique luminescent signals. Each unique bead is then complexed with capture oligos specific to a given target mRNA. This assay is designed to serve as an initial gene expression screening tool, to assist poultry scientists in identifying signaling pathways for further investigation. Validation of this assay is still ongoing and expected to be completed the following reporting cycle.
Gallardo
Objective 1. We have generated additional information in regards to resistance to IBV infections using MHC B haplotype chickens. This information allows us to have a working animal model that has been proven testing minerals as boosters of immune responses. In addition, we have continued to respond to pressing field issues in the commercial industry including avian reovirus variants, IBV associated false layer syndrome, and infectious coryza. Further, we developed an antigenic cartography computational method that can be used to understand the antigenic and genetic relatedness of diverse pathogens.
Lamont
Objective 1. A review of literature on genetics and APEC was published. Bioinformatic analyses of the splenic transcriptome revealed that innate immune pathways were differentially expressed and therefore could be potential targets to modulate resistance to APEC. Knockdown of OASL increased the amount of NDV viral RNA, and it also eliminated the difference in expression of interferon response and apoptosis-related genes between NDV-infected and noninfected cells, suggesting that OASL modulates response to NDV infection.
Objective 3. Expression patterns of chicken HDPs were determined in resistant and susceptible chicken genetic lines. ISU chicken genetic lines were reproduced and maintained and shared.
Drechsler
Objective 1: Developing new project on Immunglobulin-like receptors in the chicken (ChIR). Phylogenetic analysis and re-annotation of ChIR is in progress. Preliminary data with siRNA shows effects on ChIR-B.
Objective 3: Continuation of functional annotation of chicken genome: 20 tissues/cells in progress. DNA methylome completed for reproductive and intestinal tissues/ peripheral immune cells. RNA seq completed for all tissues, peripheral blood cells. Pending: tissue macrophages. Some samples need repeating due to QC. ATAC seq completed for intestinal, reproductive tissues and peripheral immune cells. ChIP seq optimization is ongoing for several tissues/cells.
Taylor
Objective 1: Individual and pooled samples from chickens with defined alloantigen genotypes underwent SNP analyses. Alloantigen A was associated with a region from 2,420,000 to 2,890,000 bp on chromosome 26. A candidate gene with high consistency between amino acid changing SNP and allelic differences identified the alloantigen A gene as C4BPM (complement component 4 binding protein membrane). A second alloantigen, E, is tightly linked to the A system. It was originally identified as unannotated locus, LOC101748581, but has been annotated as FCAMR, Fc fragment of IgA and IgM receptor. A similar approach was used to identify alloantigen D. A chromosome 1 region between 128,600,000 to 128,850,000 bp was associated with allelic changes and SNP. The candidate gene for alloantigen D is CD99.
Objective 3. Genetic stocks consisting of two inbred lines, four congenic lines and six line crosses are maintained for research. Stocks are typed at the MHC and other alloantigen systems.
Bielke
Objectives 2 and 3: The role of pioneer colonization of the GIT in neonatal birds was shown to have age-related effects, especially with regards to immune tolerance and innate immune function. Generally, Gram negative bacteria decrease ability of birds to respond to inflammatory events and lactic acid bacteria promote colonization with segmented filamentous bacteria, which are thought to promote beneficial innate immune function. This has been demonstrated through other experiments in which early inoculation with Gram negative bacteria increased gut permeability and susceptibility to necrotic enteritis. Gram negative inoculation promoted dendritic cell migration to gut tissue, decreased HNF1-alpha, decrease pathways associated with D-glucose, and F-gamma receptor dependent phagocytosis. Conversely, lactic acid bacteria promoted gluconeogenesis, B cell receptor signaling, Class I MHC antigen processing, and IL-1 while downregulating heterophil degranulation and MHC Class II antigen presentation. These clearly demonstrate the role of colonizing bacteria in immune system function and maturation.
Song
Objective 1: In allelic specific expressions of CD4+ T cells, we found some critical genes and CNV linked to T cell activation, T cell receptor (TCR), B cell receptor (BCR), ERK/MAPK, and PI3K/AKT-mTOR signaling pathways, which play potentially essential roles in MDV infection.
Objective 3: We investigated the antibiotic resistance profiles of Escherichia coli found in poultry litter, as well as E. coli O serogroups, virulence genes, and antimicrobial resistance genes. In this context , we examined the prevalence of antimicrobial resistance and heavy metal genes detected among isolates and identified those with a high prevalence of copper and silver, tetracycline, aminoglycosides, gentamicin, and sulphonamides.
Parcells
We cloned the chicken EZH2 gene (2 isoforms) and the chicken SATB1 and have found that these interact with Meq splice variant-encoded proteins. These data have direct implications regarding the suppression of MDV lytic gene expression, transformation and the Treg patterning of MDV-latently-infected cells. We found that the long form of Meq in CVI988 actually confers higher levels of oncogenicity to RB-1B, but that the short form of this same Meq is attenuating. We also found that Meq isoforms from higher virulence strains have increased interactions with DNA-repair proteins, suggesting that MDV evolution of virulence may involve increased somatic mutation. This year we found that serum exosomes are taken up by HD11 cells programmed to be DCs, suggesting that in vivo, serum exosomes may be important to systemic antigen presentation. We found that C4BP-A is not likely a determinant of Alloantigen A.
Jarosinski
Objective 1: Identification and characterization of chicken complement receptor-like 1 (CR1L) or complement component 4 binding protein, GPI-anchored (C4BPG) showed differences in protein sequences between different chicken lines more resistant or susceptible to MD.
Objective 2: We identified up- and down-regulation of purinergic receptors both during MDV infection, as well as between infected or transformed cells in vivo. Objective 3: We have cloned the putative chicken CR1L/C4BPG and developed monoclonal antibodies to this protein.
Swaggerty
Objective 1: We evaluation the innate immune markers from chickens selected for high (HAS) and low (LAS) antibody responses to sheep red blood cells. Differences were observed in the mRNA expression levels of CXCL8 comparing males and females from the HAS line which held true for both PBL and spleen samples. Further, mRNA expression levels for IL6, CXCL8, and CCL4 were consistently higher in spleen samples compared to the PBL in the HAS line.
Selvaraj
Objective 3: Conducted a study to identify the effects of Effects of Salmonella enterica ser. Enteritidis and Heidelberg on Host CD4+CD25+ Regulatory T Cell Suppressive Immune Responses in Chickens. S. Enteritidis and S. Heidelberg infection at 3 d of age induces a persistent infection through inducing CD4+CD25+ cells and altering the IL-10 mRNA transcription of CD4+CD25+ cell numbers and cytokine production in chickens between 3 to 32 dpi allowing chickens to become asymptomatic carriers of Salmonella after 18 dpi. A second study was conducted to identify if a Salmonella chitosan nanoparticle vaccine administration is protective against Salmonella Enteritidis in broiler birds. Chitosan nanoparticle vaccinated birds had 0.9 Log10 CFU/g decreased SE cecal loads (P<0.05) compared to control. The vaccine under study did not had any adverse effects on the bird’s BWG and FCR or the IL-1β, IL-10, IFN-γ, or iNOS mRNA expression levels. We concluded that the CNP vaccine, either as a first dose or as a booster vaccination, is an alternative vaccine candidate against Salmonella in poultry.
Cotter
Objective 2: The demonstration of heterogeneity among plasmacyte series, cells known for antibody secretion is an important step in the understanding of the complexities of immune reactions.
Impacts
- Erf Objective 2. The autoimmune disease-prone Smyth, UCD-200/206 and Obese strain chickens are important genetic models to study the cause-effect relationship between genetic susceptibility, immune function, and environmental factors in multifactorial, non-communicable diseases. Objective 3. The development of the growing feather as a dermal test-site enables study of in vivo immune system and tissue responses initiated by injected test-material in a complex vascularized tissue. Arsenault Objective 2: The results from the work comparing ACRB and modern broilers response to cocci challenge will allow us to better understand the changes to the modern broiler immune system due to selective pressures, this information will aid in formulating methods of modulating the immune response at key points in grow out to enhance the modern broiler’s resistance to disease. The chicken methylation clock provides insights into inflammatory effects on epigenetics. This can impact both growth performance of the bird as well as innate immune training and adaptive immune response. In addition, this provides another method of measuring the inflammatory status of broilers chickens, proving new insight into the resting and challenge state of the birds and their immune responses. The two collaborative feed additive mechanism of action studies both provide insight into antibiotic alternatives and alternative growth promoters. These results may serve as a target of intervention for specific inflammatory management conditions and challenges. Koci Objective 3. Once completed, this multiplex assay system will allow for members of the community to screen samples for changes in gene expression of 45 different targets, across 3 major systems (immunology, stress, and gut function). The data from these experiments will be useful in helping scientists determine the best complement of genes to assay for by RT-PCR, as well as identify experimental conditions best suited for RNAseq analysis. Gallardo Objective 1. Our recent work has provided a better understanding of 1) the primary and secondary immune response against IBV and the role of cell responses on resistance to the pathogen; 2) persistence and antigenic determinants in Avibacterium paragallinarum (AP); and 3) antigenic determinants in reovirus. Zhou Identification of genes that are associated with resistance to heat stress and Newcastle disease virus and can be used to genetic enhancement of disease resistance of chicken in adaption to hot climate; Elucidating underlying cellular mechanisms of genetic resistance to avian influenza virus in chickens could lay a great foundation for novel strategy in prevention; Understanding the molecular mechanisms of Salmonella colonization in chickens could aid in development novel strategy in improving food safety in poultry industry. Lamont Objective 1. A review paper provided access for scientists to a curated summary of literature associated with genetics and APEC response. Identification of structural and functional genetic variants associated with differential responses to pathogens laid the foundation for future studies, for rationale design of vaccines and for genetic selection to improve disease resistance in poultry. Objective 3. Information on expression of HDP in resistant and susceptible chicken lines may aid in understanding their function. Continued research with ISU chicken genetic lines was enabled. Drechsler Objective 1. Functionally annotating the chicken genome will benefit research in agricultural animals. Uncovering the location of regulatory elements and determining their interactions will provide the necessary framework to understand how regulatory networks govern gene expression and how genetic and environmental influences alter these networks to impact animal growth, health and disease susceptibility or resistance. Establishing the role of chicken immunoglobulin-like receptors will benefit agricultural and human research. Their role in immunity in chicken, and in human, association with MHC-I during disease remains unexplored. This study will further understanding of immunoglobulin-like receptors in disease resistance in MHC defined chickens, providing producers with genetic biomarkers for enhanced immunity against diseases through selective breeding. Taylor Objective 1. Genetic improvement will benefit from alloantigen gene and gene product identification. Associations between economic traits and specific alloantigen genes will be advantageous to stakeholders. Objective 3. WVU will continue producing specific MHC haplotypes and segregating alloantigen alleles in genetic stocks for collaborative studies. Bielke Altogether, this research at Ohio State U. stresses the importance of early microbial colonization on immune function and inflammation of poultry. Gram negative bacteria, which possess lipopolysaccharides, appear to negatively influence susceptibility to disease and ability of broilers to respond to inflammatory events later in life. Conversely, results suggest that lactic acid bacteria promote a favorable bacterial environment and help control inflammation in the GIT. Some results presented here suggest that pioneer colonization can affect susceptibility of broilers to necrotic enteritis caused by co-infection with Eimeria and C. perfringens, further demonstrating the importance of hatchery and parent flock management. Parent flock and hatchery microbiology should be considered critical components to directing favorable colonization of production flocks. Song Objective 1. In copy number variation analysis, we found some critical genes and CNV linked to T cell activation and key signaling pathways that which play potentially essential roles in MDV infection. Also, we found that the adipoR1 mRNA expression level was significantly increased in MD-susceptible chickens after MDV infection. The role of adiponectin in chickens will help advance the understanding of lipid metabolism in response to herpesvirus infection. Most importantly, we found that The Meq might affect the main features of tumorous cells, including proliferation, apoptosis, and invasion, suggesting that the Meq gene might play a crucial role in interfering with lymphomatous cell transformation. Parcells The identification of Meq proteins with the polycomb repressive complex and chickens SATB1 ties latency directly to cellular transformation, suggesting a very important and tractable model for Hogkin’s lymphoma. Uptake of exosomes by DC-patterned HD11 cells supports our hypothesis that serum exosomes may be important to systemic immunity. Jarosinski Objective 1: Differences in chCR1L/C4BPG between chicken lines suggest potential role of this protein in MD resistance. Objective 2: Specific purinergic receptors identified will be studied in genetic differences in chickens to MD induction or progression. Objective 3: The addition of mAbs against chCR1L/C4BPG will allow greater characterization of the immune response in chickens. Swaggerty Objective 1:. Immunological evaluation of lines of chickens selected for antibody responses provides insight into the interplay of innate and adaptive immune responses and could prove beneficial in identifying markers associated with robust immunological responsiveness. Selvaraj Objective 3: Identified a potential nanoparticle vaccine as an alternative vaccine candidate against Salmonella in poultry. Identified that S. Enteritidis and S. Heidelberg infection at 3 d of age induces a persistent infection through inducing CD4+CD25+ cells and altering the IL-10 mRNA transcription of CD4+CD25+ cell numbers and cytokine production in chickens between 3 to 32 dpi allowing chickens to become asymptomatic carriers of Salmonella after 18 dpi. Identified that a nanoparticle vaccine decreased necrotic enteritis lesions in broiler birds. Cotter Objective 2: The recognition of morphological differences among plasmacytes participating in “reactive plasmacytosis” and their similarity to multiple myeloma (MM) plasmacyte series should be of importance to those interested in basic immunological phenomena.
Publications
Peer Reviewed Papers
Akerele, G., N. Ramadan, S. Renu, R. Shanmugasundaram, G.J. Renukaradhya, and R.K. Selvaraj. 2020. In vitro characterization and Immunogenicity of chitosan nanoparticles loaded with native and inactivated extracellular proteins from a field strain of Clostridium perfringens associated with necrotic enteritis. Veterinary Immunology and Immunopathology 224:110059.
Asfor, A., S. Nazki, V.R.A.P. Reddy, E. Campbell, K.L. Dulwich, E.S. Giotis, M.A. Skinner, and A.J. Broadbent. 2021. Transcriptomic analysis of inbred chicken lines reveals infectious bursal disease severity is associated with greater bursal inflammation in vivo and more rapid induction of pro-inflammatory responses in primary bursal cells stimulated ex vivo. Viruses 13:933. doi: 10.3390/v13050933
Aston, E., A. Nayaran, S. Egaña, M. Wallach, and R.A. Gallardo. Hyperimmunized chickens produce neutralizing antibodies against SARS-CoV-2. 2021. Scientific Reports. Submitted https://www.researchsquare.com/article/rs-515320/v1
Aston, E., Y. Wang, K. Tracy, R.A. Gallardo, S.J. Lamont, and H. Zhou. 2021. Comparison of cellular immune responses to avian influenza in two genetically distinct , highly inbred chickens. Veterinary Immunology and Immunopathology 235:110233. https://www.sciencedirect.com/science/article/pii/S0165242721000519
Bai, H., Y. He, Y. Ding, Q. Chu, L. Lian, E.M. Heifetz, N. Yang, H.H. Cheng, H. Zhang, J. Chen, and J. Song. 2020. Genome-wide characterization of copy number variations in the host genome in genetic resistance to Marek's disease using next generation sequencing. BMC Genetics 21:77. doi: 10.1186/s12863-020-00884-w.
Bai, Y., P. Yuan, H. Zhang, R. Ramachandran, N. Yang, and J. Song. 2020. Adiponectin and its receptor genes expression in response to MDV infection of White Leghorns. Poultry Science, doi: 10.1016/j.psj.2020.06.004
Bai, H., Y. He, Y. Lin, Q. Leng, J.A. Carrillo, J. Liu, F. Jiang, J. Chen, and J. Song. 2020. Identification of a novel differentially methylated region adjacent to ATG16L2 in lung cancer cells using methyl-CpG binding domain protein enriched genome sequencing. Genome, doi: 10.1139/gen-2020-0071
Bortoluzzi, C., Lahaye, L., Perry, F., Arsenault, R.J., Santin, E., Korver, D.R., and Kogut, M.H. 2021. A protected complex of biofactors and antioxidants improved growth performance and modulated the immunometabolic phenotype of broiler chickens undergoing early life stress. Poultry Science 101176.
Chang, R., P. Pandey, Y. Li, C. Venkitasamy, Z Chen, R. Gallardo, B. Weimer, B. and J.M. Russell. 2020. Assessment of gaseous ozone treatment on Salmonella Typhimurium and Escherichia coli O157: H7 reductions in poultry litter. Waste Management, 117: 42-47.
Chasser, K.M., K. McGovern, A.F. Duff, B.D. Graham, W.N. Briggs, D.R. Rodrigues, M. Trombetta, E. Winson, and L.R. Bielke. 2021. Evaluation of day of hatch exposure to various Enterobacteriaceae on inducing gastrointestinal inflammation in chicks through two weeks of age. Poultry Science 100:101193.
Chasser, K.M., K. McGovern, A.F. Duff, M. Trombetta, B.D. Graham, L. Graham, W.N. Briggs, D.R. Rodrigues, and L.R. Bielke. 2021. Enteric permeability and inflammation associated with day of hatch Enterobacteriaceae inoculation. Poultry Science 100:101298.
Conradie, A.M., L.D. Bertzbach, J. Trimpert, J.N. Patria, S. Murata, M.S. Parcells, and B.B. Kaufer. 2020. Distinct polymorphisms in a single herpesvirus gene are capable of enhancing virulence and mediating vaccinal resistance. PLoS Pathogens 16(12):e1009104, doi: 10.1371/journal.ppat.1009104.
Cotter, P.F. 2021a. Erythroplastids of duck blood produced by cytokinesis, lysis, and amitosis Journal of World Poultry Research 11(2): 271-277, https://dx.doi.org/10.36380/jwpr.2021.32
Cotter, P.F. 2021b. Atypical hemograms of the commercial duck. Poultry Science100, doi: 10.1016/j.psj.2021.101248
da Silva, A.P. and R.A. Gallardo. 2020. Review: The Chicken MHC: Insights on genetic resistance, immunity and inflammation following infectious bronchitis virus infections. Viruses, https://www.mdpi.com/2076-393X/8/4/637
da Silva, A.P., R. Hauck, S.R.C Nociti, C. Kern, H.L. Shivaprasad, H. Zhou, and R.A. Gallardo. 2021. Molecular biology and pathological process of an infectious bronchitis virus with enteric tropism in commercial broilers. Viruses 13,1477. Respiratory Diseases Special Edition. https://www.mdpi.com/1999-4915/13/8/1477#
da Silva, A.P., C. Giroux, H.S. Sellers, A. Mendoza-Reilley, S. Stoute, and R.A. Gallardo. 2021. Characterization of an IBV isolated from commercial layers suffering from false layer syndrome. Avian Diseases. https://doi.org/10.1637/aviandiseases-D-21-00037
Del Vesco, A.P., H.J. Jang, M.S. Monson, and S.J. Lamont. 2021. Role of chicken oligoadenylate synthase like gene during in vitro Newcastle disease virus infection. Poultry Science, doi.org/10.1016/j.psj.2021.101067
Dulwich, K.L., A. Gray, A. Asfor, S. Giotis, M. Skinner, and AJ Broadbent. 2020. The stronger downregulation of in vitro and in vivo innate antiviral responses by a very virulent strain of infectious bursal disease virus (IBDV), compared to a classical strain, is mediated, in part, by the VP4 protein. Frontiers in Cellular and Infection Microbiology 10.315. doi: 10.3389/fcimb.2020.00315
Egaña-Labrin, S., C. Jerry, H.J. Roh, A.P. da Silva, C. Corsiglia, B. Crossley, D. Rejmanek, and R.A. Gallardo. 2021. Avian reoviruses of the same genotype induce different pathology in chickens. Avian Diseases, Accepted.
Emami, N.K, and R.A. Dalloul. 2021. CENTENNIAL REVIEW: Recent developments in host-pathogen interactions during necrotic enteritis in poultry. Poultry Science 100:101330.
Emami, N.K., A. Calik, A., M.B. White, E.A. Kimminau, and R.A. Dalloul. 2021.Managing broilers gut health with antibiotic-free diets during subclinical necrotic enteritis. Poultry Science 100:101055.
Erf, G.F., G. Le Pape, S. Rémy, and C. Denesvre. 2020. Mardivirus infection and persistence in feathers of a chicken model harboring local autoimmune response. Microorganisms 8(10):1613. https://doi.org/10.3390/microorganisms8101613
Felfoldi, B., H. Wang, N. Nuthalapati, R. L. Taylor, Jr., J. D. Evans, S. L. Branton, and G. T. Pharr. 2021. Expression of chicken leukocyte cell-derived chemotaxin 2 in the embryonic bursa of Fabricius. Int. J. Poult. Sci. 20: 43-47 https://doi.org/10.3923/ijps.2021.43.47
French, C.E., M.A. Sales, S.J. Rochell, A. Rodriguez, and G.F. Erf. 2020. Local and systemic inflammatory responses to lipopolysaccharide in broilers: new insights using a two-window approach. Poultry Science 99:6593-6605. https://doi.org/10.1016/j.psj.2020.09.078
Garcia, G., Jr, A. Sharma, A. Ramaiah, C. Sen, A. Purkayastha, D.B. Kohn, M.S. Parcells, S. Beck, H. Kim, M.A. Bakowski, M.G. Kirkpatrick, L. Riva, K.C. Wolff, B. Han, C. Yuen, Ulmert D, Purbey PK, Scumpia P, Beutler N, Rogers TF, Chatterjee AK, Gabriel G, Bartenschlager R, Gomperts B, C.N. Svendsen, U.A.K. Betz, R.D. Damoiseaux, and V. Arumugaswami. 2021. Antiviral drug screen identifies DNA-damage response inhibitor as potent blocker of SARS-CoV-2 replication. Cell Reports 35(1):108940. doi: 10.1016/j.celrep.2021.108940
Glenn, H., G.J. Mullenix, and G.F. Erf. 2021. Effect of low crude protein diet with and without Spirulina platensis inclusion on white blood cell profiles in broilers. Discovery 21:38-44. (Undergraduate Journal Publication)
Gonzales-Viera, O., F. Carvallo-Chaigneau, E. Blair, D. Rejmanek, O. Erdogan-Bamac, K. Sverlow, A. Figueroa, R.A. Gallardo, and A. Mete. 2021. Infectious bronchitis virus prevalence, characterization and strain identification in California backyard chickens. Avian Diseases DOI: 10.1637/aviandiseases-d-20-00113
Guo, Y., W. He, H. Mou, L. Zhang, J. Chang, S. Peng, A. Ojha, R. Tavora, M.S. Parcells, G. Luo, W. Li, G. Zhong, H. Choe, M. Farzan, and B.D. Quinlan. 2021. An engineered receptor-binding domain improves the immunogenicity of multivalent SARS-CoV-2 vaccines. mBio 12(3):e00930-21, doi: 10.1128/mBio.00930-21
Han, Y., S. Renu, V. Patil, J. Schrock, N. Feliciano-Ruiz, R. Selvaraj, and G.J. Renukaradhya. 2020. Immune response to salmonella enteritidis infection in broilers immunized orally with chitosan-based salmonella subunit nanoparticle vaccine. Frontiers in immunology 11:935.
Han, Y., S. Renu, J. Schrock, K.Y. Acevedo-Villanueva, B. Lester, R. Selvaraj ,and G.J. Renukaradhya. 2020.Temporal dynamics of innate and adaptive immune responses in broiler birds to oral delivered chitosan-based Salmonella subunit nanoparticle vaccine. Veterinary Immunology and Immunopathology, https://doi.org/10.1016/j.vetimm.2020.110111
Jang, H.-J., Monson, M., Kaiser., M., Lamont, S.J. 2020. Induction of chicken host defense peptides within disease-resistant and -susceptible lines. GENES 11:1195; doi:10.3390/genes11101195
Lee, A., G.C. Dal Pont, M. Battaglia, R. Arsenault, and M. Kogut. 2021. Role of JAK-STAT pathway in chicks fed with chestnut tannins. Animals 11(2):337.
Lee, A., G.C. Dal Pont, M.B. Farnell, S. Jarvis, M. Battaglia, R. Arsenault, and M. Kogut. 2021. Supplementing chestnut tannins in the broiler diet mediates a metabolic phenotype of the ceca. Poultry Science 100:47-54.
Mon, K.K.Z., C. Kern, G. Chanthavixay, Y. Wang, and H. Zhou. 2021. Tolerogenic immunoregulation towards Salmonella Enteritidis contribute to colonization persistence in young chicks. Infection and Immunity. doi: 10.1128/IAI.00736-20.
Monson, M.S. and S.J. Lamont. 2021. Genetic resistance to avian pathogenic Escherichia coli (APEC): current status and opportunities. Avian Pathology, doi: 10.1080/03079457.2021.1879990
Monson, M.S., B.L. Bearson,, M.J. Sylte, T. Looft, S.J. Lamont, S.J., and S.M.D. Bearson. 2021.Transcriptional response of blood leukocytes from turkeys challenged with Salmonella enterica serovar Typhimurium UK1. Veterinary Immunology and Immunopathology 232: doi.org/10.1016/j.vetimm.2020.110181
Mortada, M., D.E. Cosby, R. Shanmugasundaram, and R.K. Selvaraj. 2020. In vivo and in vitro assessment of commercial probiotic and organic acid feed additives in broilers challenged with Campylobacter coli. Journal of Applied Poultry Research 29:435-446. doi:10.1016/j.japr.2020.02.001
Mullenix G. J, E.S. Greene, N.K. Emami, G. Tellez-Isaias, W.G. Bottje, G.F. Erf, M.T. Kidd, and S. Dridi. 2021. Spirulina platensis inclusion reverses circulating pro-inflammatory (chemo)cytokine profiles in broilers fed low-protein diets. Frontiers in Veterinary Science https://doi.org/10.3389/fvets.2021.640968
Mushi, J., G.H. Chiwanga, E. Mollel, M. Walugembe, R.A. Max, P. Msoffe, R.A. Gallardo, T. Kelly, S. Lamont, J. Dekkers, H. Zhou, and A. Muhairwa. 2021. Antibody response, viral load, viral clearance and growth rate in Tanzanian free-range local chickens infected with lentogenic Newcastle disease virus. 2021. Journal of Veterinary Medicine and Animal Health 13:98-105.
Mushi, J., G.H. Chiwanga, E.N. Amuzu-Aweh, M. Walugembe, R.A. Max, S.J. Lamont, T.R. Kelly, E.L. Mollel, P.L. Msoffe, J. Dekkers, R. Gallardo, H. Zhou, and A.P. Muhairwa. 2020. Phenotypic variability and population structure analysis of Tanzanian free-range local chickens. BMC Veterinary Research 16:360. doi: 10.1186/s12917-020-02541-x.
Neerukonda SN, N.A. Egan, J. Patria, I. Assakhi, P. Tavlarides-Hontz, S. Modla, E.R. Muñoz, M.B. Hudson, and M.S. Parcells. 2020. A comparison of exosome purification methods using serum of Marek's disease virus (MDV)-vaccinated and -tumor-bearing chickens. Heliyon 6(12):e05669. doi: 10.1016/j.heliyon.2020.e05669
Nuthalapati, N., T.A. Burks, R.L. Taylor, Jr., P.B. Siegel, and G. . Pharr. 2021. Protein tyrosine kinase gene expression profiles in the embryonic bursa of Fabricius of chicken lines selected for high and low antibody responses. International Journal of Poultry Science 20:173-178 https://doi.org/10.3923/ijps.2021.173.178
Omara, I.I., C.M. Pender, M.B. White, and R.A. Dalloul. 2021. The modulating effect of dietary beta-glucan supplementation on expression of immune response genes of broilers during a coccidiosis challenge. Animals 11:151.
Overbey, E.G., T.T. Ng, P. Catini, L.M. Griggs, P. Stewart, S. Tkalcic, R.D. Hawkins, and Y. Drechsler: 2021. Transcriptomes of an array of chicken ovary, intestinal, and immune cells and tissues. Frontiers in Genetics 12:664424. doi: 10.3389/fgene.2021.664424
Patel, R.T., B.M. Gallamoza, P. Kulkarni, M.L. Sherer, N.A. Haas, E. Lemanski, I. Malik, K. Hekmatyar, M.S. Parcells, and J.M. Schwarz. 2021. An examination of the long-term neurodevelopmental impact of prenatal zika virus infection in a rat model using a high resolution, longitudinal MRI approach. Viruses 13(6):1123. doi: 10.3390/v13061123
Raddatz, G., R.J. Arsenault, B. Aylward, R. Whelan, F. Böhl, and F. Lyko. 2021. A chicken DNA methylation clock for the prediction of broiler health. Communications Biology 4(1):1-8.
Renu, S., Y. Han, S. Dhakal, Y.S. Lakshmanappa, S. Ghimire, N. Feliciano-Ruiz, S. Senapati, B. Narasimhan, R. Selvaraj, and G.J. Renukaradhya. 2020. Chitosan-adjuvanted Salmonella subunit nanoparticle vaccine for poultry delivered through drinking water and feed. Carbohydrate Polymers 243:116434.
Saelao, P., Y. Wang, G. Chanthavixay, V. Yu, R.A. Gallardo, J. Dekkers, S.J. Lamont, T. Kelly, and H. Zhou. 2021. Distinct transcriptomic response to Newcastle disease virus infection during heat stress in chicken tracheal epithelial tissue. Scientific Reports 11:7450. https://www.nature.com/articles/s41598-021-86795-x.pdf
Shanmugasundaram, R., A. Markazi, M. Mortada, T.T. Ng, T.J. Applegate, L.R. Bielke, and R.K. Selvaraj. 2020. Effect of synbiotic supplementation on caecal Clostridium perfringens load in broiler chickens with different necrotic enteritis challenge models. Poultry Science 99(5):2452-2458. doi:10.1016/j.psj.2019.10.081
Taylor, R.L., Jr. 2021. The 100 most cited papers from Poultry Science’s centennial. Poultry Science 100:, https://doi.org/10.1016/j.psj.2021.101256
Taylor, R.L., Jr. and D. Jones. 2021. A century of progress 1921-2021. Poult. Sci. 100:101073 https://doi.org/10.1016/j.psj.2021.101073
Tong, Z.W.M., A.C. Karawita, C. Kern, H. Zhou, J.E. Sinclair, L. Yan, K.Y. Chew, S. Lowther, L. Trinidad, A. Challagulla, K.A. Schat, M.L. Baker, and K.R. Short. 2021. Primary chicken and duck endothelial cells display a differential response to infection with highly pathogenic avian influenza virus. Genes 12 (6):901.
Troxell, B., M. Mendoza, R. Ali, M. Koci, and H. Hassan. 2020. The attenuated Salmonella enterica serovar Typhimurium, strain NC983, is immunogenic, and protective against virulent Typhimurium challenges in mice. Vaccines 8 (4), 646. https://doi.org/10.3390/vaccines8040646
Vega-Rodriguez, W., H. Xu, N. Ponnuraj, H. Akbar, T. Kim, and K.W. Jarosinski. 2021. The requirement of glycoprotein C (gC) for interindividual spread is a conserved function of gC for avian herpesviruses. Scientific Reports 11(1):7753.
Vega-Rodriguez W., N. Ponnuraj, M. Garcia, and K.W. Jarosinski. 2021. The requirement of glycoprotein C for interindividual spread is functionally conserved within the Alphaherpesvirus genus (Mardivirus), but not the host (Gallid). Viruses 13(8):1419
Wilkinson, N.G., R.T. Kopulos, L.M. Yates, W.E. Briles, and R.L. Taylor, Jr. 2021. Research Note: Rous sarcoma growth differs among congenic lines containing major histocompatibility (B) complex recombinants. Poultry Science 100:
Zhang, L., X. Wei, R. Zhang, M. Koci, D. Si, B. Ahmad, and H. Guo. 2020. C-terminal amination of a cationic anti-inflammatory peptide improves bioavailability and inhibitory activity against LPS-induced inflammation. Frontiers in Immunology 15. doi 10.3389/fimmu.2020.618312
Zhao, C.F., X. Li, B. Han, L.J. Qu, C.J. Liu, J. Song, N. Yang, and L. Lian. 2020. Knockdown of the Meq gene in Marek's disease tumor cell line MSB1 might induce cell apoptosis and inhibit cell proliferation and invasion. Journal of Integrative Agriculture 19:2767-2774. doi.org/10.1016/S2095
Published Abstracts
Arsenault, R.J. Strategic Modulation of Intestinal Microbiome: An Immunologist Perspective. Poultry Science Annual Meeting. 2021, July, Virtual Conference.
Arsenault, R.J. Kinomics: Regulation of the Metabolome. Poultry Science Annual Meeting. 2021, July, Virtual Conference.
Arsenault, R.J. The Immunometabolic Interface Between Host and Microbiota. Animal Nutrition Conference of Canada. 2021, May, Virtual Conference.
Beck, C. N., J. Santamaria, M. A. Sales, and G. F. Erf. 2021. Primary and recall immune responses to autogenous Salmonella vaccine or Salmonella lipopolysaccharide administration in Light-brown Leghorn pullets. Poult. Sci. PSA Virtual Conference.
Bielke, L.R.. A.F. Duff, K. M. Chasser, W.N. Briggs, and K.M. Wilson. “Modeling necrotic enteritis: Applying lessons learned.” Poultry Science Association Annual Meeting, Virtual Conference, July 2021.
Blue, C.E.C., E.A. Kimminau, M.B. White, N.K. Emami, and R.A. Dalloul. 2021. Effects of a phytogenic feed additive on broilers during a necrotic enteritis challenge. International Poultry Scientific Forum (Virtual), Atlanta, GA.
Chasser, K.M., A.F. Duff, K.E. McGovern, M. Trombetta, and L.R. Bielke. “Comparison of chick quality, health, and inflammation from two hatchery environments.” Poultry Science Association Annual Meeting, Virtual Conference, July 2021.
Cueva, Justin R., Nicholas Egan, Imane Assakhi, Phaedra Tavlarides-Hontz, and Mark S. Parcells. Sequential interactions of meq proteins with polycomb repressive complex proteins in marek’s disease virus latency. The 13th International Symposium on Marek’s Disease and Avian Herpesviruses, June 2021.
Dallakoti, Aksana, Sabarinath Neerukonda, Phaedra Travlarides-Hontz, and Mark S. Parcells. Transcriptomic and proteomic analysis of exosomes released by Marek’s disease virus transformed t-cell lines. The 13th International Symposium on Marek’s Disease and Avian Herpesviruses, June 2021.
Duff, A.F., K.M. Chasser, K.E. McGovern, M. Trombetta, and L.R. Bielke. Adaptation of cell culture assay measuring fluorescent quantification of β-D-Glucuronidase activity for assessment of ileal granulocyte degranulation in tissue scrapings. Poultry Science Association Annual Meeting, Virtual Conference, July 2021.
Evans, R.D., J. Santamaria, and G.F. Erf. 2021. Evaluation of the toxigenicity of lipopolysaccharide associated with chicken hepatopathy. AAAP Virtual Conference.
Koci, M. Connecting the Microbiome to Immune Function Through Metabolomics. 3rd Microbiome Movement – Animal Health & Nutrition. Online. October 2020 (International meeting).
McGovern, K.E., J.C. Bielke, A.F. Duff, A. Calvert, K.M. Chasser, and L.R. Bielke. Measuring Eimeria oocysts viability via auto-fluorescence following anticoccidial treatment. Poultry Science Association Annual Meeting, Virtual Conference, July 2021.
Miller, Joshua, Kyle Moskowitz, Samuel Keating, Abhyudai Singh, Prasad Dhurjati, Andelé Conradie, Benedikt B. Kaufer, Phaedra Travlarides-Hontz, and Mark Parcells. The development of agent-based and mathematical models for Marek’s disease virus (mdv) lytic and latent infections. The 13th International Symposium on Marek’s Disease and Avian Herpesviruses, June 2021.
Parcells, Mark S., Joshua S. Miller, Erin Gollhardt, Aksana Dallakoti, Shannon Modla, Eric Muñoz, Matthew B. Hudson, and Ryan J. Arsenault. Effect of serum exosomes from vaccinated and protected and tumor-bearing chickens on immune function. The 13th International Symposium on Marek’s Disease and Avian Herpesviruses, June 2021.
Patria, Joseph, Nirajan Bhandari, Phaedra Travlarides-Hontz, Andelé Conradie, Benedikt B. Kaufer, and Mark S. Parcells. Evaluation of the effect of Meq isoform on Marek’s disease virus (MDV) pathogenicity. The 13th International Symposium on Marek’s Disease and Avian Herpesviruses, June 2021.
Santamaria, J., C.N. Beck, M.A. Sales, and G.F. Erf. 2021. Inflammatory and antibody responses to intradermally administered autogenous Salmonella vaccine isolates and content-matched Salmonella lipopolysaccharide. Poultry Science association Annual Conference (Virtual).
Taylor, R.L., Jr., W. Drobik-Czwarno, A. Wolc, and J.E. Fulton. 2021. Candidate genes for A and E blood group systems in the chicken. Poultry Science 100(E-Suppl. 1):54.
Trombetta, M., K.M. Chasser, A.F. Duff, K.E. McGovern, D.R. Rodrigues, D. Jeffery, D.J. Shafer, and L.R. Bielke. Effect of probiotics on early microbial colonization in day of hatch ducklings. Poultry Science Association Annual Meeting, Virtual Conference, July 2021.
Wisser-Parker, Kristy, Kristen Wooten, Phaedra Talverides-Hontz, and Mark S. Parcells. Assessment of the role of IRG1 and itaconate on Marek’s disease virus (NDV) infection. The 13th International Symposium on Marek’s Disease and Avian Herpesviruses, June 2021.
Book Chapters:
Erf, G.F. (in press). Autoimmune diseases of poultry. Pp. xxx-xxx. In: Avian Immunology, 3rd Ed.. K.A. Schat, B. Kaspers, T. Goebel, L. Vervelde, Eds., Elsevier, London, San Diego
Lamont, S.J., J.C.M. Dekkers, A. Wolc, and H. Zhou. (in press). Immunogenetics and the mapping of immunological functions. Pp. xxx-xxx. In: Avian Immunology. K.A. Schat, B. Kaspers, T. Goebel, L. Vervelde, Eds., Elsevier, London, San Diego
6.3 Thesis/Dissertation
Chasser, Kaylin. Effect of day of hatch inoculation with Enterobacteriaceae on inflammation and enteric permeability in broilers. Ph.D. dissertation, May 2021. Ohio State University. Supervisor: Lisa R. Bielke
Johnson, Casey. A Kinomic Analysis of the Immunometabolic Effects of Antibiotic Alternatives in Necrotic Enteritis Disease Model. Ph.D. Dissertation. 2021. University of Delaware. Supervisor: Ryan J. Arsenault
White, Mallory B. In ovo and feed application of probiotics or synbiotics and response of broiler chicks to post-hatch necrotic enteritis. PhD Dissertation. 2021 Virginia Tech. Supervisor: Rami A. Dalloul