SAES-422 Multistate Research Activity Accomplishments Report

Sections

Status: Approved

Basic Information

Participants

Elizabeth HInes, Penn State University; John Parrish, University of Wisconsins-Madison; Andre Furugen, University of Illinois; Rod Geisert, University of Missouri; Dave Miller, University of Illinois; Adele Turzillo, Penn State University; Bret White, University of Nebraska-Lincoln; Jeremy Miles, USDA, MARC; Kara Stewart, Purdue University; Lea Rempel, USDA, MARC ; Lee Johnston, University of Minnesota; Lidia Sbaraini Arend, University of Illinois; Rob Knox, University of Illinois; Raquel Fernandez, University of Illinois; Carol Madox, University of Illinois; Mark Mirando, USDA; Jean Feugang, Mississippi State University; Jason Ross, Iowa State University;

The meeting was called to order at 1:00 pm by Elizabeth Hines via a zoom connection.  We were welcomed by Adele Turzillo, Department head at Penn State Department of Animal Sciences who described the campus and overview of the department.

John Parrish presented an opportunity to the group to submit a special collection on Pig Reproduction and the history of the committee to the Journal of Animal Science.  He is also now the Editor and Chief of the Image Gallery for ASAS and welcomed people to submit images and videos for teaching.

Station Reports: Wisconsin by John Parrish; Nebraska by Brett White, Missouri - Rod Geisert; Illinois - Rob Knox, Purdue - Kara Stewart, Penn State - Elizabeth Hines;

Lastly for the first day Troy Ott and Joy Pate discussed the Center for reproductive biology and health at Penn state. It is cross campus, have a group meeting every other week with ideas on grants wth critic and comments given.  They also have journal clubs for graduate students and post docs.  Has physical facilites with group shared equipment and lab space.

The meeting then came to an end for Monday May 17.

The meeting resummed on May 18 at 9:00.

Station Reports: MARC Nebraska - Lea Rempel; Iowa State University - Jason Ross

Buisness meeting:

Business meeting started at 10 AM. The symposium was a no go this year and so will be next year, 2022 on "Managing the Highly Prolic Sow." The motion was moved and seconded and approved.  The comitted for the symposium is Kara Stewart, Tim Safranski, Lea Rempel and Jason Ross. It was suggested that a component of the symposium should be to go over basics of reproductive management, such as why and how to use the basics of pg600 and matrix etc. The symposium will be on May 24, 2022, Tuesday with some station reports to follow and finish with a half day of buisness meeting and reports on May 25, 2022.  Will have a opening event at Fair Oaks on Monday evening, May 23, 2022. 

Mark Mirando next presented the NIFA report and the new administrator changes at USDA.

Recruitment was next discussed and Amy Switzer Desaulers at UNL Vet Science was suggested as a target. This was followed by John Parrish discussing further the opportunity for a special collection in Journal of Animal Science.  The committee then thanked Elizabeth Hines for hosting the on line meeting. Lastly Jeremy Miles was appointed as next years secretary.

Station Reports: Illinois - Dave Miller; Other stations had nothing to report

Many thanks were expressed to Elizabeth Hines for chairing the committee and hosting the meeting.

Overall it was noted that Covid had major impacts on the ability of the comittee to work with other members on research in the last year and it is expected to improve in the next year.

John Parrish was moved from Secretary to Chair of the committee and the meeting was adjourned at 11:30 AM.

 

Accomplishments

Accomplishments supporting objectives:

  1. Objective 1, 3 and 4 - Impact of heat stress on the boar and impact on placental gene expression (USDA-MARC, Wisconsin)
  2. Objective 1 and 4 - developed predictors and mediation methods for sperm quality in agricultural species (Wisconsin, Miss. State).
  3. Objective 2 and 4 - managing growth and puberty development in replacement gilts (USDA-MARC, NC)
  4. Objective 2 and 4 - mapping genetic variants associated with age at puberty and sow fertility (USDA-MARC, Missouri)
  5. Objective 3 - GnRH2 in the boar (UNL, USDA-MARC
  6. Objective 3 - GnRH2 in the gilt ovary (UNL, USDA-MARC)
  7. Objective 2 and 4- effects of heat stress on the hepatic and ovarian proteome in gilts (Iowa State, USDA-MARC)
  8. Objective 5 - Extension program targeted to translating science into usable information for swine farms (Purdue, Missouri, Illinois)
  9. Objective 3 - Investigating survivability in swine (Purdue, Iowa)
  10. Objective 1, 3 and 4 - determined the effects on the sperm proteome of a mild acute increase in scrotal temperature in pigs with over 800 proteins identified (Illinois, Wisconsin)
  11. Objective 1, 3 and 4 - identified 4 potential biomarkers found in sperm that may provide early indication of fertility reduction due to elevated environmental temperature (Illinois, Wisconsin
  12. Objective 1 - Number of sperm in AI and impacts on fertilization (Illinois, Purdue, Wisconsin)
  13. Objective 2 - In utero heat stress alters postnatal phenotypes in swine (Missouri, Purdue, Iowa)
  14. Objective 3 and 4 - Gene Editing to Investigate Role of Conceptus Factors in Establishment of Pregnancy (Missouri, Tx)
  15. In support of Objective 1, researchers validated a novel microscopic imaging approach for high-throughput evaluation of sperm quality (MSSTATE and University of Illinois) and demonstrated the usefulness of nanotechnology-based approaches to improving sperm quality (MSSTATE and Wisconsin University).

Impacts

  1. Identification of biological markers of semen quality improves long-term development of mitigation strategies to heat stress in boars
  2. Improved understanding of puberty onset in gilts lead to long-term development of selection tools and improve selection rates for gilts in pig production
  3. Metabolic profile of the uterine environment during early embryonic life provides clues to understanding and mitigation of early embryonic death
  4. Combined, research and publications aid producers in trouble shooting poor conception rates in reproductive herd
  5. Biomarker identification critical step to developing prediction tools and testing for heat stress related infertility in boars, gilts and sows
  6. Demonstration that GnRH-II and its receptor are important to sperm and follicular function,
  7. Enhance development of novel technologies for sperm selection to improve male fertility.
  8. New understanding of how in utero heat stress impact the immune system in the neonate and newborn pig as well as postnatal phenotype..
  9. Changing paradigm of how the conceptus directly impacts implantation.
  10. Nutrititional and floor cooling pads were shown to prevent heat stress in boars
  11. Identification of fertility biomarkers in boars and pre-pubertal gilts that allow for early selection of animals, improving efficiency of the sow herd and boar stud.
  12. Development of an integrated computerized sensing system for reproductive management of the swine breeding herd.
  13. Dietary profiles influence growth and development of gilts.
  14. Seasonal alterations to chromatin modifiers within the placenta are impacted by breeding season and season of semen collection.

Publications

Basic Science and Technology

  1. Wijesena, H.R., Kachman, S.D., Lents, C.A., Riethoven, J.J., Trenhaile-Grannemann, M.D., Safranski, T.J., Spangler, M.L., Ciobanu, D.C. 2020. Fine mapping genetic variants associated with age at puberty and sow fertility using Sowpro90 genotyping array. Journal of Animal Science. 98(10):1-12. https://doi.org/10.1093/jas/skaa293.
  2. Azain, M., Cline, P.M., Tsai, T.C., Lents, C.A., Stelzleni, A.M., Dove, C.R. 2020. Interaction of dietary carbohydrate and fat on glucose metabolism in growing pigs. Research Square. pp. 22. https://doi.org/10.21203/rs.3.rs-48869/v1.
  3. Nastasijevic, I., Schmidt, J.W., Boskovic, M., Glisic, M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M., Bosilevac, J.M. 2020. Seasonal prevalence of Shiga toxin-producing Escherichia coli on pork carcasses for three steps of the harvest process at two commercial processing plants in the United States. Applied and Environmental Microbiology. 87(1):e01711-20. https://doi.org/10.1128/AEM.01711-20.
  4. Chaudhari, J., Liew, C., Workman, A.M., Riethoven, J.M., Steffen, D., Sillman, S., Vu, H.L.X. 2020. Host transcriptional response to persistent infection with a live-attenuated porcine reproductive and respiratory syndrome virus strain. Viruses. 12(8):817. https://doi.org/10.3390/v12080817.
  5. Vande Pol, K.D., Tolosa, A.F., Shull, C.M., Brown, C.B., Alencar, S.A., Lents, C.A., Ellis, M. 2021. Effect of drying and/or warming piglets at birth under warm farrowing room temperatures on piglet rectal temperature over the first 24 h after birth. Translational Animal Science. txab060. https://doi.org/10.1093/tas/txab060.
  6. Kerns K, Sharif M, Zigo M, Miller D, Sutovsky S. 2020. Sperm capacitation-induced zinc efflux is necessary for increased proteasomal activity and release from oviduct glycans of the sperm reservoir. Int J Mol Sci 21:2121.
  7. Sharif M, Kerns K, Sutovsky P, Bovin N, Miller. DJ  2021. Progesterone induces porcine sperm release from oviduct glycans in a proteasome-dependent manner. Reproduction. 161:449-457.
  8. Johnson, JS, JM Maskal, AW Duttlinger, KR. Kpodo, BR McConn, CJ Byrd, BT Richert, JN Marchant-Forde, DC Lay, SD Perry, MC Lucy, TJ Safranski.   In utero heat stress alters the postnatal innate immune response of pigs. J. Anim. Sci. 98 (12):1-13.
  9. Bernhard, CJ, KG Sharp, TJ Safranski, WR Lamberson and MC Lucy.   Reproduction and reproductive tract morphology of male and female pigs whose mothers were heat stressed during the second month of gestation.  J. Anim Sci. 98(11): 1-12.
  10. Maskal, J.M., AW Duttlinger, KR Kpodo, BR McConn, CJ Byrd, JN Marchant-Forde, DC Lay, SD Perry, MC Lucy, TJ Safranski, JS Johnson.   Evaluation and mitigation of the effects of in utero heat stress on piglet growth performance, postabsorptive metabolism, and stress response following weaning and transport.  J. Anim. Sci., 98(9):1-13.
  11. Ciccarelli M, Giassetti MI, Miao D, Oatley MJ, Robbins C, Lopez-Biladeau B, Waqas MS, Tibary A, Whitelaw B, Lillico S, Park CH, Park KE, Telugu B, Fan Z, Liu Y, Regouski M, Polejaeva IA, Oatley JM. Donor-derived spermatogenesis following stem cell transplantation in sterile NANOS2knockout males. Proc Natl Acad Sci U S A. 2020 Sep 29;117(39):24195-24204.  PMID: 32929012
  12. Park CH, Jeoung YJ, Uh KJ, Park KE, Bridge J, Powell A, Li J, Pence L, Zhang Y, Liu T, Sun H, Gu Y, Shen Y, Wu J, Belmonte J-C, Telugu BP*. Extraembryonic Endoderm (XEN) Cells Capable of Contributing to Embryonic Chimeras Established from Pig Embryos. Stem Cell Reports. 2021 Jan 12;16(1):212-223. doi: 10.1016/j.stemcr.2020.11.011. PMID: 33338433
  13. Geisert R.D., A.E. Meyer, C.A. Pfeiffer, D.N. Johns, K. Lee, K.D. Wells, T.E. Spencer, and R.S. Prather. 2021. Gene Editing to Investigate Role of Conceptus Factors in Establishment of Pregnancy. Reproduction 161: R79-R88.
  14. Pfeiffer C.A., A.E. Meyer, K.E. Brooks, P.R. Chen, J. Milano-Foster, L.D. Spate, J.A. Benne, R. Cecil, M.S. Samuel, L.A. Ciernia, M.F. Smith, K.D. Wells, T.E. Spencer, R.S. Prather, and D. Geisert. 2020. Ablation of conceptus PTGS2 provides a new understanding of early pregnancy events in the pig. Biol Reprod 102:475-488. doi.org/10.1093/biolre/ioz192.
  15. Cho J, Uh K, Ryu J, Fang X, Bang S, Lee K. Development of PCR based approach to detect potential mosaicism in porcine embryos. Journal of Animal Reproduction and Biotechnology. 2020 Dec. 35. 4.
  16. Uh K, Ryu J, Farrell K, Wax N, Lee K. TET family regulates the embryonic pluripotency of porcine preimplantation embryos by maintaining the DNA methylation level of NANOG. Epigenetics. 2020 Nov;15(11):1228-1242. doi: 10.1080/15592294.2020.1762392. Epub 2020 May 13.
  17. Tumova L, Zigo M, Sutovsky P, Sedmikova M, Postlerova P (2020) Is the ubiquitin-proteasome system involved in the degradation of porcine beta-microseminoprotein during sperm capacitation? Int. J. Mol. Sci., 21(11):4151.
  18. Kerns K, Sharif M, Zigo M, Xu W, Hamilton L, Sutovsky M, Ellersieck M, Drobnis EZ, Oko R, Miller D, Sutovsky P (2020) Sperm cohort-specific zinc signature acquisition and capacitation-induced zinc flux regulate sperm-oviduct and sperm-zona pellucida interactions. Int. J. Mol. Sci., 21(6):2121; Special Issue on Advances in Molecular Regulation of Spermatozoa Function,
  19. Zigo M, Maňásková-Postlerová P, Zuidema D, Kerns K, Jonáková V, Tůmová L, Bubeníčková F, Sutovsky P (2020) Porcine model for the study of sperm capacitation, fertilization and male fertility. Cell Tissue Res., 380(2):237-262.
  20. Zuidema D, Sutovsky P (2020) The domestic pig as a model for the study of mitochondrial inheritance. Cell Tissue Res., 80(2):237-262. Special Issue on Animal Models,
  21. Pfeiffer, C.A., A.E. Meyer, K.E. Brooks, P.R. Chen, J.M. Foster, L.D. Spate, J.A. Benner, R. Cecil, M.S. Samuel, L.A. Ciernia, M.F. Smith, K.D. Wells, T.E. Spencer, R.S. Prather. 2020. Ablation of conceptus PTGS2 provides a new understanding of early pregnancy events in the pig. Biol. Reprod. Doi: 10/.1093/biolre/ioz192 PMID: 31616930, PMCID: PMC7523696.
  22. Stoian, A., R.R.R. Rowland, V. Petrovan, M. Sheahan, M.S. Samuel, K.M. Whitworth, K.D. Wells, J. Zhang, B. Beaton, M. Cigan, R.S. Prather. 2020. The use of cells from ANPEP knockout pigs to evaluate the role of aminopeptidase N (APN) as a receptor for porcine deltacoronavirus (PDCoV). Virology. 2020 Feb; 541:136-140. doi: 10.1016/j.virol.2019.12.007. Epub 2019 Dec 24. PMID: 32056711, PMCID: PMC7112016
  23. Chen, P.R., L.D. Spate, E.C. Leffeler, J.A. Benne, R.F. Cecil, T.K. Hord, R.S. Prather. 2020. Removal of hypotaurine from porcine embryo culture medium does not impair development of in vitro fertilization or somatic cell nuclear transfer-derived embryos at low oxygen tension. Molecular Reproduction and Development https:/doi: 10.1002/mrd.23393. PMID 32495478, PMCID: PMC7496716.
  24. Cecil, R.F., J.A. Benne, P.R. Chen, T.K. Hord, L.D. Spate, M.S. Samuel, R.S. Prather. 2020. Chemical stimulation of hypoxia in somatic cell nuclear transfer donor cells permits metabolic reprogramming and improved SCNT efficiency. Molecular Reproduction and Development https:/doi: 10.1002/mrd.23392 PMID 32558023, PMCID: PMC7496615.
  25. Lucas, C.G., A.M. Spate, M.S. Samuel, L.D. Spate, W.C. Warren, R.S. Prather, K.D. Wells. 2020. A novel swine sex-linked marker and its application across different mammalian species. Transgenic Research doi: 10.1007/s11248-020-00204-z. PMID: 32607872, PMCID: PMC7423754
  26. Ostedgaard, L.S., M.P. Price, K.M. Whitworth, M.H.A. Alaiwa, A.J. Fischer, A. Warrier, M. Samuel, L.D. Spate, P.D. Allen, B.M. Hilkin, G.S. R. Ibarra, M.E. Ortiz, B.J. Goodelll, S.E. Mather, L.S. Powers, M.R. Stroik, N.D. Gansmer, C.E. Hippee, K. Zarei, J.A. Goeken, T.R. Businga, E. A. Hoffman, D.K. Meyerholz, R.S. Prather, D.A. Stoltz, M.J. Welsh. 2020. Lack of submucosal glands impairs respiratory host defenses in pigs. eLife 2020;9:e59653. DOI: https://doi.org/10.7554/eLife.59653. PMID:33026343, PMCID: PMC7541087
  27. Koppes, E., B.K. Redel, M.A. Johnson, K.J. Shvorak, L. Ghaloul-Gonzalez, M.E. Yates, D.W. Lewis, S.M. Gollin, Y. Wu, S.E. Christ, M. Yerle, A. Leshinski, L.D. Spate, J. Benne, S. Murphy, M.S. Samuel, E.M. Walters, S.A. Hansen, K.D. Wells, J. Vockley, R.S. Prather, R.D. Nicholls. 2020. A porcine model of phenylketonuria generated by CRISPR/Cas9 genome editing. JCI Insight doi: 10.1172/jci.insight.141523. PMID: 33055427 PMCID: PMC7605535.

 

Boar Performance

  1. Mills, K.M., Aryal, U.K., Sobreira, T., Minton, A.M., Casey, T., and K.R. Stewart. 2020. Shotgun proteome analysis of seminal plasma differentiate boars by reproductive performance.  Theriogenology 157: 130-139.  doi.org/10.1016/j.theriogenology.2020.07.013
  2. Lents, C.A., Supakorn, C., DeDecker, A.E., Phillips, C.E., Boyd, R.D., Vallet, J.L., Rohrer, G.A., Foxcroft, G.R., Flowers, W.L., Trottier, N.L., Salak-Johnson, J.L., Bartol, F.F., Stalder, K.J. 2020. Dietary lysine-to-energy ratios for managing growth and pubertal development in replacement gilts. Applied Animal Science. 36(5):701-714. https://doi.org/10.15232/aas.2020-02016. DOI: https://doi.org/10.15232/aas.2020-02016
  3. Rempel, L.A., Parrish, J.J., Miles, J.R. 2020. Genes associated with chromatin modification within the swine placenta are differentially expressed due to factors associated with season. Frontiers in Genetics. 11:1019. https://doi.org/10.3389/fgene.2020.01019.
  4. Belstra, B.A., Willenburg, K.L., Gómez-López, D.H., Knox, R.V., Stewart, K.R., 2020. Effects of the number of sperm and site of uterine semen deposition on conception rate and the number of embryos in weaned sows receiving a single fixed-time insemination. Journal of Animal Science 98.
  5. Kerns K, Jankovitz J, Minton A, Kuster C, Robinson J, Sutovsky P (2020) Relationship between the length of sperm tail mitochondrial sheath and fertility traits in boars used for artificial insemination. Antioxidants, 9(11):1033. doi: 10.3390/antiox9111033
  6. Parrish JJ, PREDICITION OF FERTILITY IN MALES, US Patent App. 16/024,335; Patent granted May 2020.

 

Genetics and Genomics

  1. Johnson, J.S., Stewart, K.R., Safranski, T.J., Ross, J.W. and L.H. Baumgard.  2020.  In utero heat stress alters postnatal phenotypes in swine.  Theriogenology, 154:110-119. doi.org/10.1016/j.theriogenology.2020.05.013
  2. Wijesena, H.R., SD Kachman, CA Lents, JJ Riethoven, MD Trenhaile-Grannemann, TJ Safranski, ML Spangler, DC Ciobanu. Fine mapping genetic variants associated with age at puberty and sow fertility using SowPro90 genotyping array.   J. Anim. Sci. 98(10): 1-12.
  3. Johnson, J.S., KR Stewart, TJ Safranski, JW Ross, LH Baumgard. In utero heat stress alters postnatal phenotypes in swine. 2020. Theriogenology, 154: 110-119

 

Sow and Gilt Performance

  1. Belstra, B.A., Willenburg, K.L, Gómez-López, D.H., Knox, R.V., and K.R. Stewart. 2020. Effects of number of sperm and site of uterine deposition on conception rate and number of embryos in weaned sows receiving a single fixed time insemination.  J Anim Sci.,98:9, 1-10. doi:10.1093/jas/skaa260
  2. Suarez-Trujillo, A., Senn, K., Teeple, K., Casey, T., and K.R. Stewart. 2020. Technical Note: A standardized model to study effects of varying 24 h colostrum dose on postnatal growth and development. TAS, txaa212, doi.org/10.1093/tas/txaa212.
  3. Leonard, S.M., Xin, H., Brown-Brandl, T.M., Ramirez, B.C., Johnson, A.K., Dutta, S., Rohrer, G.A. 2021. Effects of farrowing stall layout and number of heat lamps on sow and piglet behavior. Applied Animal Behaviour Science. 239:105334. https://doi.org/10.1016/j.applanim.2021.105334.

 

Teaching and extension

  1. Mills, K.M., Schinckel, A.P., Casey, T.M., and K.R. Stewart. 2020. Evaluation of on-farm indicators of gilt reproductive performance potential at 21 days of age.  TAS, txaa210, doi.org/10.1093/tas/txaa210.  

 

 

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