SAES-422 Multistate Research Activity Accomplishments Report
Sections
Status: Approved
Basic Information
- Project No. and Title: NE1834 : Genetic Bases for Resistance and Immunity to Avian Diseases
- Period Covered: 10/01/2019 to 10/13/2020
- Date of Report: 11/10/2020
- Annual Meeting Dates: 10/13/2020 to 10/14/2020
Participants
Bob Taylor, West Virginia University Gisela Erf, University of Arkansas Ramesh Selvaraj, University of Georgia Yvonne Drechsler, Western University Lisa Bielke, Ohio State University Ryan Arsenault, University of Delaware Mark Parcells, University of Delaware Robert Beckstead, North Carolina State University Huaijun Zhou, UC Davis Matt Koci, North Carolina State University Keith Jaronsinski, University of Illinois Janet Fulton, Hyline Dalloul, Rami, University of Georgia Marcia Miller, City of Hope, CA Rodrigo Gallardo UC Davis Kirk Klasing, UC Davis Jiuzhou Song, University of Maryland Sue Lamont, Iowa State Chris Ashwell, North Carolina State University Christi Swaggerty, USDA-ARS Juan Carlos Rodriguez, Atlantic Veterinary College Henk Parmentier, Wageningen University (did attend some of the time but did not give a presentation) Muquarrab Qureshi USDA-ARS Guest Tina Dalgaard, Aarhus University Denmark PhD Students-Staff-Post doctoral scholars: Theros Ng, UGA (Dr. Drechsler’s lab) Kaylin Chasser, M.S (Dr. Bielke’s lab) Absentees: Calvin Keeler, University of Delaware Mohammad Heidari, USDA ARS Athens Cari Hearn, USDA ARS East Lansing Mark Berres, University of Wisconsin Solomon O. Odemuyiwa,, University of Missouri
Accomplishments
Erf
Objective 2. A vitiligo treatment study, conducted in AR using the Smyth line autoimmune vitiligo model, revealed that the target tissue (the growing feather; GF) can be repeatedly microinjected without impact on GF growth and development. Examination of innate immunity in systemic sclerosis/scleroderma-prone UCD 200/206 chickens revealed altered responses to microbial components and poly-clonal T cell activators in UCD200/206 chickens. The GF-cutaneous test developed to examine local cellular/tissue responses to pulp-injection of test-materials was successfully adapted for use in young commercial broilers, yielding new knowledge on the local (GF) and systemic (blood) LPS-induced inflammatory response.
Objective 3. Genetic stocks, 3 MHC-matched lines of the Smyth autoimmune vitiligo model, two lines (220 & 206) of the UCD chicken model for systemic sclerosis/scleroderma, and the Obese strain chicken model for Hashimoto’s thyroiditis, were maintained at AR for research. AR continued to refine and expand the growing feather as an in vivo test-tube system to study innate and adaptive immune responses in poultry.
Arsenault
Objective 1: N/A
Objective 2: We elucidated a potential mechanism of why we see differing prevalence of Salmonella serovars in poultry in the field. The serovars trade off infectivity/invasiveness for persistence. S. Enteritidis is more invasive and elicit a greater inflammatory response but may lead to greater clearance. S. Heidelberg is less invasive but may evade immunity better for longer-term infectivity. A combination of purified yeast cell wall fractions appears to be the better formulation for immune modulation and resistance to necrotic enteritis. They provide greater potency in addition to a synergistic effect. Our collaborations with the Mellata group at Iowa State and Johnson group at Minnesota showed that non-tradition probiotics, SFB, and host-adaptive probiotics have positive effects on poultry gut health and are promising preventative approaches in poultry production.
Beckstead
Objective:
To identify the SNP markers associated with genetic resistance to H. meleagridis infection in commercial.
Koci
For 2020 we have not made any contributions to Objective 1. For Objective 2 we have characterized the expression of innate immune cells from turkey poults following infection with turkey astrovirus type-2 to better understand the nature of the immune suppression induced by this virus. We have also used machine learning to assist with our understanding of the differences in the immune responses of genetic lines of chickens selected for differences in their antibody responses. We have not made any contributions to Objective 3 for 2020.
Miller
For Objective 1, we have made significant advances in defining the sequence of MHC-Y and the NOR regions on chicken Chromosome 16. We have advanced understanding of the polymorphic nature of chicken MHC class I molecules revealing an unusual arrangement of polymorphic residues. We have also contributed to efforts defining the function of MHC-Y in immune responses and bacterial colonization. For Objective 2, we have provided the those interested in chicken genetics a sample means for defining MHC-Y genotypes in chickens. This method is suitable for typing large numbers of birds in a short time.
Ashwell
Objective 2
The NC/Ashwell group has increased the characterization of the adaptive immune functions and the influence of genetic and environmental factors contributing to these processes by studying lines of White Leghorn chickens, High Antibody Selected (HAS) and Low Antibody Selected (LAS), that have been continuously divergently selected for 5-day post-injection antibody titer to injection with sheep red blood cells (SRBC) for nearly 50 years. Differential gene expression was analyzed combining traditional statistics and machine learning to obtain signature gene lists for functional analysis, which revealed differences in energy production and cellular processes between lines and with SRBC injection.
Gallardo
We have been able to generate good basic information in regards to resistance to IBV infections using MHC B haplotype chickens. This information allows us to have a working animal model that has been proven testing minerals as boosters of immune responses. In addition, we have been able to respond to pressing issues to the industry i.e. avian reovirus variants, IBV associated false layer syndrome and infectious coryza.
Dalloul
Necrotic enteritis, is one of the major enteric diseases that negatively impacts the poultry industry. The increasing ban on the use of antibiotic growth promoters in poultry production has resulted in higher incidence of necrotic enteritis outbreaks worldwide. Our research demonstrated that supplementation of natural additives established a unique taxonomic and functional signature in the ileal microbiota, which was accompanied by better performance and reduced pathology of broilers.
Taylor
Objective 1. DNA sequence and SNP analyses of samples from chickens with defined alloantigen genotypes revealed an association between alloantigen A and a chromosome 26 region from 2,420,000 to 2,890,000 bp. Among four possible candidate genes, high consistency between amino acid changing SNP and allelic differences identified the alloantigen A gene as C4BPA (complement component 4 binding protein alpha). Alloantigen E, tightly linked to the A system received tentative identification as an unannotated locus, LOC101748581.
Objective 3. Genetic stocks consisting of two inbred lines, two congenic lines and five line crosses were maintained for research. Stocks are typed at the MHC and other alloantigen systems.
Bielke
Objectives 2 and 3 – The role of pioneer colonization of the GIT in neonatal birds was shown to have age-related effects, especially with regards to immune tolerance and innate immune function. Generally, Gram negative bacteria decrease ability of birds to respond to inflammatory events and lactic acid bacteria promote colonization with segmented filamentous bacteria, which are thought to promote beneficial innate immune function. This has been demonstrated through other experiments in which early inoculation with Gram negative bacteria increased gut permeability and susceptibility to necrotic enteritis. Gram negative inoculation promoted dendritic cell migration to gut tissue, decreased HNF1-alpha, decrease pathways associated with D-glucose, and F-gamma receptor dependent phagocystosis. Conversely, lactic acid bacteria promoted gluconeogenesis, B cell receptor signaling, Class I MHC antigen processing, and IL-1 while downregulating heterophil degranulation and MHC Class II antigen presentation. These clearly demonstrate the role of colonizing bacteria in immune system function and maturation.
Lamont
Objective 1. We characterized the splenic transcriptome of commercial brown-egg birds infected with NDV. We conducted a joint-tissue analysis of the transcriptomes of lung, trachea and Harderian gland of Leghorn and Fayoumi birds infected with NDV. We determined the expression of genes of the eIF2 family in spleen of Leghorn and Fayoumi birds infected with NDV.
Objective 3. We characterized the thymic transcriptome of birds exposed to heat, injected with LPS, or both. We defined the expression of 20 host defense peptides in chick embryo fibroblasts and bone-marrow-derived cell cultures after LPS or poly I:C exposure. We maintained the eight ISU chicken genetic lines, and transferred them into a new building. We assessed cell-surface expression of MHC class I antigens on red blood cells of the ISU genetics lines.
Parmentier
Hygienic conditions appear to determine the level of specific immune responses (specific antibodies), natural and natural auto antibodies, aswell as innate immunity (complement dependent lysis) in young growing broilers. In hygienic (clean) conditions immune responses were considerably lower. Behaviour was accompanied by levels of specific and natural antibodies. ‘Aggressive’ birds showed higher IgG levels compared to ‘passive’ birds that were characterized by higher IgM levels. This suggests that levels of immune parameters may be helpful as breeding goal against misbehaviour of poultry, but also point to the risk that breeding for high immunocompetence may result in enhanced misbehaviour.Birds (and mammals) show high contents of antibodies binding ‘self-antigens’, especially when these antigens are conjugated to danger signals such as phophoryl choline (PC). Innate immune cells show training (non-specific memory) when earlier challenged with non-related PAMP.
Rodriguez
We have demonstrated that FA supplementation modulated B lymphocytes response and improved their innate immune antiviral and proinflammatory response pathways; their effect is evident when he have used a low virulent pathotype serotype I modified live IBDV vaccine that was able to trigger and mount an immune response in chickens B lymphocytes without affecting B-cell viability. Besides, we demonstrated the capacity of IBDV to trigger a potent innate immune response in chicken cells (DF-1, HD-11, and DT-40); we found the beneficial effect of Vit D supplementation as an immunomodulator.
Huaijun Zhou
Objective 1: For Genomics to improve Poultry project, we conducted naïve natural vNDV exposure trials and estimated genetic parameters of different traits associated with disease resistance for genomic selection in African ecotypes. For epigenomic study, we functionally annotate regulatory elements in chicken bursa and identified regulatory elements changes associated with genes related cell cycle and receptor signaling of lymphocytes in chickens.
Objective 2: For Salmonella infection, we found that perturbation in metabolic pathways related to arginine and proline metabolism as well as TCA cycle contributed to Salmonella persistence in chickens.
Drechsler
Objective 3:
Functional Annotation of the Chicken Genome: Yvonne Drechsler in collaboration with Dr. Hawkins at the University of Washington is funded to functionally annotate the chicken genome in several immune cells and tissues. Tissues and cells have been collected and are in various processing stages. DNA sequencing is completed for most cells and tissues, RNA seq is mostly complete. ATAC seq and ChiP seq are in optimization stages.
Resistance to respiratory pathogens, including coronavirus-induced infection and clinical illness in chickens has been correlated with the B (MHC) complex and differential ex vivo macrophage responses. RNA seq and ATAC seq have been analyzed in SPF chickens infected with IBV. Tissue and viral strain specific epigenetic changes have been observed. Final analysis is pending.
Song
In allelic specific expressions of CD4+ T cells, we found some critical genes and CNV linked to T cell activation, T cell receptor (TCR), B cell receptor (BCR), ERK/MAPK, and PI3K/AKT-mTOR signaling pathways, which play potentially essential roles in MDV infection. However, the effects of parent-of-origin have not been detected on survival days after MDV infection. Moreover, an interaction between MDV infection and linc-GALMD1 was also observed. The lincRNA could repress MDV gene expression during MDV infection, which may help uncover the roles of linc-GALMD1 as a viral gene regulator and tumor suppression by regulating immune responses to MDV infection. Interestingly, we found that the adipoR1 mRNA expression level was significantly increased in MD-susceptible chickens after MDV infection.
Parcells
This year we compared the ability of vaccine-associated exosomes (VEX) to increase the efficacy of HVT against an RB1B challenge. We found that injection of 5 x 109 exosomes at 10 days post-hatch increased the survival of chickens challenged at hatch after in ovo HVT vaccination (p<0.002), and that injection at 5 and 10 dph showed a trend of increasing survival (p<0.07), but that treatment at 5 dph alone did not positively affect survival. Conversely, we examined the effect of tumor-associated serum exosomes (TEX) on MD incidence and survival of HVT/SB1-vaccinated chickens (in ovo) and found no significant change in survival due to the severity of the low passage RB1B challenge.
Role of Meq Splice Variants in Interactions with Polycomb Repressive Complex Proteins: The finding of interaction of Meq splice variant-derived proteins, proteins that accumulate as MDV establishes latency, with the Polycomb Repressive complex (PRCs), ties MDV latency directly to a pathway associated with cellular transformation and tumor progression.
Modeling of MDV Evolution of Virulence: We have established the following NetLogo Agent-based models of: (1) Chicken spleen infection by MDV, (2) Model for MDV Reactivation from Latency, and (3) MDV Infection and Spread in chicken embryo fibroblasts (CEF).
Klasing
Objective 1 - The effect of an enzyme blend (xylanase, amylase and protease; XAP) on intestinal histology, immunological response and performance of Cobb500 broilers following a mild coccidial challenge was examined. The challenged birds had increased pro-inflammatory cytokines (IL-6, IL-1β; P < 0.05) in the intestine and decrease growth and feed efficiency. XAP reduced inflammatory responses as measure by indices of the hepatic and systemic acute phase response and immunopathology and improved performance.
Objective 2 - A chicken knockout line (KO) lacking B lymphocytes were examined for their resistance to a challenge with Salmonella enterica. When challenged the KO chickens lost less body weight than the controls, had similar mortality, but higher indices of intestinal inflammation.
Impacts
- Erf Objective 2. The autoimmune disease-prone Smyth, UCD-200/206 and Obese strain chickens are important genetic models to study the cause-effect relationship between genetic susceptibility, immune function, and environmental factors in multifactorial, non-communicable diseases. Objective 3. The development of the growing feather as a dermal test-site enables study of in vivo immune system and tissue responses initiated by injected test-material in a complex vascularized tissue. Arsenault Delaware Objective 2: The identification of pathogenesis between two serovars of Salmonella important to human food safety. The differences center on the timing and intensity of innate immune inflammatory responses, which may be important in how quickly or effectively birds do or do not clear Salmonella. We determined that a combination purified yeast cell wall fractions prevent NE better than crude YCW due to greater engagement of immune responses. The recommendation will be for at least two purified fractions to be included in feed, rather than crude YCW. SFB are a beneficial probiotic due to their activation of immune responses and enhancement of gut barrier function. Host-adapted probiotics are effective at improving gut health in a way similar to antibiotics. Beckstead Objective 1 Currently there are no treatments or vaccines available to treat or prevent histomoniasis. Data from this research will aid turkey genetics companies in selecting for pedigree lines that are resistant to the disease, thereby preventing the financial losses and animal welfare issues associated with histomoniasis. Koci Objective 2 The data reported for 2020 ad to our understanding of how gene expression regulates the immune response. Miller Objective 1. The genomic sequence determination for MHC-Y in the RJF reference genome will provide a base for further studies devoted to MHC-Y immunogenetics. Data are emerging supporting the hypothesis that genes within the highly polymorphic MHC-Y region are involve in guiding immune responses in chickens. Data are emerging suggesting that MHC-Y contributes to the genetics governing colonization of chickens by Campylobacter jejuni. Objective 3. There is now a much simpler method for MHC-Y genotyping that will make it possible to type large numbers of birds quickly. This will make it possible to far more easily compare sample sets across experimental challenge trials and different strains of chickens. NC- Ashwell Objective 1. The results of this work provide insight into energetic resource needs and allocations in response to genetic selection and antigen exposure in HAS and LAS which may help explain phenotypic differences observed in antibody response between lines. Gallardo Objective 1. Our work provides further insight into the increased susceptibility of 335/B19 birds to infectious bronchitis. In addition we have taken advantage of the MHC resistant / susceptible model to investigate the effect of Zn and Mn on the immunity of chickens as direct application of this model. In terms of infectious diseases affecting poultry we have understood the persistence, vaccine protection and bacterial genotyping for Avibacterium paragallinarum and we have understood the pathobiology and of IBV causing FLS and some of the repercussions of the use of vaccination to control this syndrome. Objective #2: We have proved that avian reovirus variants (ARV) are able to infect and cause lymphoid depletion in Bursa of Fabricius and thymus suggesting that this virus causes B and T cell immunodeficiency. Dalloul Objective 2. This work is of significant impact showing the possibility to control and modify the immune responses, microbial profile and metabolism of chickens by dietary supplementation of natural additives. By modifying such responses, this approach led to alleviating the negative impact of necrotic enteritis under filed-like research settings, which remains to be proved in commercial field conditions. Taylor Objective 1. Knowing alloantigen genes and gene products will assist in genetic improvement. Stakeholders benefit form knowledge of associations between alloantigen genes and characteristics with commercial value. Objective 3. Defined genetic stocks will enable further discovery of genes that affect traits having economic importance. Bielke Objective 2. Altogether, this research stresses the importance of early microbial colonization on immune function and inflammation of poultry. Gram negative bacteria, which possess lipopolysaccharides, appear to negatively influence susceptibility to disease and ability of broilers to respond to inflammatory events later in life. Conversely, results suggest that lactic acid bacteria promote a favorable bacterial environment and help control inflammation in the GIT. Some results presented here suggest that pioneer colonization can affect susceptibility of broilers to necrotic enteritis caused by co-infection with Eimeria and C. perfringens, further demonstrating the importance of hatchery and parent flock management. Parent flock and hatchery microbiology should be considered critical components to directing favorable colonization of production flocks. Lamont Objective 3. Maintenance of genetic resources enables future studies in immunogenetics in chickens. Identification of genes and pathways responding to infection with bacterial or viral pathogens, especially those differing between resistant and susceptible genetic lines, will aid our understanding of resistance mechanisms, and identify candidate genes for genetic selection to improve response. This will result in enhanced animal health and welfare, improved food safety, and better-informed management practices. Parmentier Objective 2. Hygienic conditions appear to determine the level of specific immune responses (specific antibodies), natural and natural auto antibodies, as well as innate immunity (complement dependent lysis) in young growing broilers. Modulation or management of the environment (housing) have a profound effect on immune responsiveness. Levels of immune parameters may be helpful as breeding goal against misbehaviour of poultry, but also point to the risk that breeding for high immunocompetence may result in enhanced misbehaviour. (lower) levels of these self-binding antibodies may predict upcoming metabolic diseases, and as such may act as a predictor. ‘Vaccination’ to enhance or maintain levels of these antibodies might add to prevent metabolic and inflammatory related diseases. Importance of hygienic conditions in housing and feed? Juan Carlos Rodriguez Objective 2. The chicken Ang4 might have a potential bactericidal effect against intestinal pathogenic microbes such Clostridium perfringens; however, independent of the modulating the intestinal microbiota and the innate immunity, their effect on angiogenesis and tissue repair need to be evaluated. Our work is contributing to the understanding of the activation pathways of innate immunity induced by IBDV. Zhou Objective 1: Identification of genes that are associated with resistance to heat stress and Newcastle disease virus and can be used to genetic enhancement of disease resistance of chicken in adaption to hot climate. Objective 2: Understanding the molecular mechanisms of Salmonella colonization in chickens could aid in development novel strategy in improving food safety in poultry industry. Drechsler Objective 1. Characterization of gene regulatory elements in the chicken genome will aid in the selection of markers for disease resistance in breeding. An ideal mechanism for controlling disease in poultry is to breed birds with natural resistance. We are identifying mechanisms for this resistance. Innate immune functions, particularly activation of macrophages, has consistently shown to be different in disease resistant versus susceptible birds. We are investigating the role of the host epigenome in immune evasion of viruses and disease resistance and susceptibility to develop a deeper understanding of the genetic processes involved. Song Objective 1. The role of adiponectin in chickens will help advance the understanding of lipid metabolism in response to herpesvirus infection. The first report on the relationship between virus infection and mitochondria in chickens will provide a unique clue in understanding pathogenesis and tumorigenesis due to viral infection. Parcells Objective 1. Role of polycomb repressive complex proteins: The finding of interaction of Meq splice variant-derived proteins, proteins that accumulate as MDV establishes latency, with the Polycomb Repressive complex, ties MDV latency directly to a pathway associated with cellular transformation and tumor progression. This work also connects MDV-induced lymphomagenesis to EBV-associated Hodgkin’s lymphoma, as well as other human leukemias and lymphomas. Meq mutations and innate selection: Our finding that mutations in the C-terminus of the Meq oncoprotein, which have been associated with the MDV virulence level, affect the innate sensing and signaling in infected cells. This is the first direct, causal association of meq mutation and a mechanism affecting one aspect of MDV virulence; namely, the evasion of the innate immune system. In follow up to this, we have submitted Meq immunoprecipitations from MDV-infected spleen cell lysates from an in vivo study, as well as tumor cell lines from JM10, RB-1B, RB-1B-based recombinants, and MK and TK strain-transformed T-cell lines to further characterize Meq-binding proteins during lytic and latent infections. Presence of MDV mRNAs in Exosomes of Vaccinated and Protected Chickens: Our finding that viral mRNAs, but not virus DNA, are present in serum exosomes in vaccinated and protected chickens suggests that these exosomes are conferring systemic immunity through CTL-priming by macrophages and dendritic cells that have taken up these exosomes and expressed these proteins. This observation, coupled with our small RNA transcriptomic analysis, may provide the very basis of systemic immune protection elicited by MD vaccines. The finding that we can, in fact, generate mature dendritic cells with IL4, GM-CSF, LPS and IFNpermits a careful and methodical analysis of the role exosomes play in mediating long-term, systemic CTL responses. Klasing Objective 1- The use of enzymes to lower the pathogenicity of coccidia will be a useful adjunct to vaccination approaches in the control of this disease. Objective 2- Understanding the importance of B lymphocytes relative to T lymphocytes in the protective response to SE challenges is important for the development of efficacious vaccines.
Publications
Peer reviewed
Alber, A., Morris, K.M., Bryson, K.J., Sutton, K., Monson, M.S., Chintoan-Uta, C., Borowska, D., Lamont, S.J., Schouler, C., Kaiser, P., Stevens, M., Vervelde, L. 2020. Avian pathogenic Escherichia coli (APEC) strain-dependent immunomodulation of respiratory granulocytes and mononuclear phagocytes in CSF1R-reporter transgenic chickens. Front. Immunol. https://doi.org/10.3389/fimmu.2019.03055
Bai H, He Y, Ding Y, Chang S, Zhang H, Chen J, Song J. Parent-of-origin has no detectable effect on survival days of Marek's disease virus infected White Leghorns. Poult Sci. 2019 98:4498-4503 doi: 10.3382/ps/pez209
Bai H, He Y, Ding Y, Chu Q, Lian L, Heifetz EM, Yang N, Cheng HH, Zhang H, Chen J, Song J. Genome-wide characterization of copy number variations in the host genome in genetic resistance to Marek's disease using next generation sequencing. BMC Genet. 2020 21:77. doi: 10.1186/s12863-020-00884-w.
Bai Y, Yuan, P., Zhang, H. Ramachandran, Yang, N. Song J. Adiponectin and its receptor genes expression in response to MDV infection of White, Leghorns. Poultry Science. doi: 10.1016/j.psj.2020.06.004
Bai, H.; He, Y.; Ding, Y.; Carrillo, J.A.; Selvaraj, R.K.; Zhang, H.; Chen, J.; Song, J. Allele-Specific Expression of CD4+ T Cells in Response to Marek’s Disease Virus Infection. Genes 2019, 10, 718. He Y, Han B, Ding Y, Zhang H, Chang S, Zhang L, Zhao C, Yang N, *Song J. Linc-GALMD1 Regulates Viral Gene Expression in the Chicken. Front Genet. 2019 10:1122. doi: 10.3389/fgene.2019.01122. eCollection 2019.
Beckstead RB, Anderson K and McDougald LR. 2020. Oviduct fluke (Prostagonimus macrorchis) found inside a chicken egg in North Carolina. Avian Diseases. 64: 352–353. https://doi.org/10.1637/aviandiseases-D-20-00021
Blakey, B. Crossley, A.P. Da Silva, D. Rejmanek, C. Jerry, R.A. Gallardo, S. Stoute. Infectious Bronchitis Virus Associated with Nephropathy in Diagnostic Cases from Commercial Broiler Chickens in California. Avian Diseases 2020 64:482-489 doi: 10.1637/0005-2086-64.4.482
Bolek, K., and K. Klasing. 2019. The effects of vaccination with keyhole limpet hemocyanin or oral administration of Salmonella enterica serovar Enteritidis on the growth performance of immunoglobulin knockout chickens. Poultry science 98(9):3504-3513.
Buttemer, W.A., B.A. Addison, and K.C. Klasing. 2020. The energy cost of feather replacement is not intrinsically inefficient. Journal of Zoology 98(2):142-148.
Calik A, Omara II, White MB, Evans NP, Karnezos TP, Dalloul RA. Dietary non-drug feed additive as an alternative for antibiotic growth promoters for broilers during a necrotic enteritis challenge. Microorganisms 7:257. 2019.
Calik A, Omara II, White MB, Li W, Dalloul RA. Effects of dietary direct fed microbial supplementation on performance, intestinal morphology and immune response of broiler chickens challenged with coccidiosis. Frontiers in Veterinary Science 6:463. 2019.
Chadwick EV, Beckstead RB. 2020. Two blackhead disease outbreaks in commercial turkey flocks were potentially exacerbated by poor poult quality and coccidiosis. Avian Diseases https://doi.org/10.1637/aviandiseases-D-20-00052
Chadwick EV, Malheiros RD, Beckstead RB. Early inoculation with Histomonas meleagridis has limited effects on broiler breeder growth and egg production and quality. Poultry Science 2020 Sep;99(9):4242-4248. doi: 10.1016/j.psj.2020.05.020
Chadwick EV, Rahimi S, Grimes J, Pitts J and Beckstead RB. 2020. Sodium bisulfate feed additive aids broilers in growth and intestinal health during a coccidiosis challenge. Poultry Science 99:5324-5330
Chanthavixay, G., Kern, C., Wang, Y., Saelao, P., Lamont, S.J., Gallardo, R.A., Rincon, G. and Zhou, H., 2020. Integrated Transcriptome and Histone Modification Analysis Reveals NDV Infection Under Heat Stress Affects Bursa Development and Proliferation in Susceptible Chicken Line. Frontiers in Genetics, 11, p.1176.
Chanthavixay, G., Kern, C., Wang, Y., Saelao, P., Lamont, S.J., Gallardo, R.A., Rincon, G., Zhou, H. 2020. Integrated transcriptome and histone modification analysis reveals NDV infection under heat stress affects bursa development and proliferation in susceptible chicken line. Front. Genet. 11:567812. doi: 10.3389/fgene.2020.567812
Chu, Q.; Ding, Y.; Cai, W.; Liu, L.; Zhang, H.; Song, J. Marek’s Disease Virus Infection Induced Mitochondria Changes in Chickens. Int. J. Mol. Sci. 2019, 20, 3150 doi: 10.3390/ijms20133150
da Silva, A., Schat, K. A., & Gallardo, R. A. (2019). Cytokine responses in tracheas from MHC congenic chicken lines with distinct susceptibilities to infectious bronchitis virus. Avian Diseases. 2020 64:36-45. doi: 10.1637/0005-2086-64.1.36.
da Silva, A.P., Aston, E.J., Chiwanga, G.H., Birakos, A., Muhairwa, A.P., Kayang, B.B., Kelly, T., Zhou, H. and Gallardo, R.A., 2020. Molecular Characterization of Newcastle Disease Viruses Isolated from Chickens in Tanzania and Ghana. Viruses, 12(9), p.916.
Deist, M. S., Gallardo, R. A., Dekkers, J., Zhou, H., & Lamont, S. J. (2020). Novel combined tissue transcriptome analysis after lentogenic Newcastle disease virus challenge in inbred chicken lines of differential resistance. Frontiers in Genetics, 11, http://review.frontiersin.org/review/506097/18/453015#/tab/History
Deist, M.S., Gallardo, R.A., Dekkers, J.C.M., Zhou, H. Lamont, S.J. 2020. Novel combined tissue transcriptome analysis after lentogenic Newcastle disease virus challenge in inbred chicken lines of differential resistance. Frontiers Genet. 11:11. doi: 10.3389/fgene.2020.00011
Del Vesco, A.P., Kaiser, M.G., Monson, M.S., Zhou, H., Lamont, S.J.. 2020. Genetic responses of inbred chicken lines illustrate importance of eIF2 family and immune-related genes in resistance to Newcastle disease virus. Scientific Reports 10:6155. https://doi.org/10.1038/s41598-020-63074-9
Downs, C.J., N.A. Dochtermann, R. Ball, K.C. Klasing, and L.B. Martin. 2020. The effects of body mass on immune cell concentrations. The American Naturalist 195(1):107-114.
Echeveryy H, Alizadeh M, Yitbarek A, Slominsky B, Rodriguez-Lecompte, JC. 2020. Immune Response of Chicken B cells to Yeast-derived products treated with Lytic Enzyme. In press British Poult. Sci. 21:1-6. doi: 10.1080/00071668.2020.1817328
Elad O, Uribe-Diaz S, Losada-Medina D, Yitbarek A, Sharif S and Rodriguez-Lecompte JC. 2020.Epigenetic effect of folic acid (FA) on the gene proximal promoter area and mRNA expression of chicken B-cell as antigen presenting cell. Br Poult Sci. 2020 14:1-9. doi: 10.1080/ 000 71668.2020.1799332
Emami NK, Calik A, White MB, Kimminau EA, Dalloul RA. Effects of probiotics and multi-component feed additives on microbiome, gut barrier and immune responses in broiler chickens during subclinical necrotic enteritis. Frontiers in Veterinary Science. 2020 https://doi.org/10.3389/fvets.2020.572142
Emami NK, Calik A, White MB, Young M, Dalloul RA. Necrotic enteritis: The role of tight junctions and mucosal immune responses in alleviating the effects of the disease. Microorganisms 7:231. 2019 doi: 10.3390/microorganisms7080231
Ferreira, H. L., Reilley, A. M., Goldenberg, D., Ortiz, I. R., Gallardo, R. A., & Suarez, D. L. (2020). Protection conferred by commercial NDV live attenuated and double recombinant HVT vaccines against virulent California 2018 Newcastle disease virus (NDV) in chickens. Vaccine, 38(34), 5507-5515.
Figueroa, T. Derksen, S. Biswas, A. Nazmi, D. Rejmanek, B. Crossley, P.Pandey, R.A. Gallardo. Persistence of LPAI and HPAI in Reused Poultry Litter, Effects of Litter Amendment Use and Composting Temperatures. J Appl. Poult. Res. 2020 in press
French, C. E., M. A. Sales, S. J. Rochell, A. Rodriguez, and G. F. Erf. 2020. Local and systemic inflammatory responses to lipopolysaccharide in broilers: new insights using a two-window approach. Poult. Sci. (in press).
Gallardo, R. A. Invited Review: Infectious bronchitis virus variants: Generation, Surveillance, Control and Prevention. Australian Journal of Veterinary Sciences. 2019. In press.
Gallardo, R. A., da Silva, A. P., & Rebollo, M. A. (2020). Effects of Amino acid-bound Zinc and Manganese Feed Additives on MHC Haplotype Chickens Challenged with Infectious Bronchitis Coronavirus. Avian Diseases. 2020 64:451-456. doi: 10.1637/aviandiseases-D-20-00031
Gallardo, R. A., Da Silva, A. P., Egaña-Labrin, S., Stoute, S., Kern, C., Zhou, H., ... & Corsiglia, C. (2020). Infectious Coryza: Persistence, Genotyping, and Vaccine Testing. Avian diseases, 64:157-165. https://www.aaapjournals.info/doi/abs/10.1637/aviandiseases-D-19-00184
Hassan, H.M., M. Mendoza, M. Rezvani, M. D. Koci, A. N. Dickey, and E. H. Scholl. 2020). Complete Genome Sequences of Lactobacillus strains, C25 and P38, Isolated from Chicken Cecum. Microbiol. Resour. Announc. Sep, 9 (39) e00501-20. DOI: 10.1128/MRA.00501-20.
Jaime J, Vargas-Bermúdez D.S., Yitbarek A., Reyes J, and Rodríguez-Lecompte JC. 2019. Differential immunomodulation effect of vitamin D (1,25 (OH)2 D3) on the innate immune response in different types of cells infected with IBDV. Poult. Sci. 99:4265-4277
Johnson, C.N., Hashim, M.M., Bailey, C.A., Byrd, J.A., Kogut, M.H., Arsenault, R.J. Feeding of yeast cell wall extracts during a necrotic enteritis challenge enhances cell growth/survival and immune signaling in the jejunum of broiler chickens. 2020. Poultry Science. 99, 2955-2966.
Kim, T.H., C. Kern, and H. Zhou. 2020. Knockout of IRF7 Highlights its Modulator Function of Host Response Against Avian Influenza Virus and the Involvement of MAPK and TOR Signaling Pathways in Chicken. Genes 11, 385; doi:10.3390/genes11040385
Krieter, A., N.P. Ponnuraj, and K.W. Jarosinski. 2020. Expression of the conserved herpesvirus protein kinase (CHPK) of Marek’s disease alphaherpesvirus in the skin reveals a mechanistic importance for CHPK during interindividual spread in chickens. J Virol. 94(5):e01522-19. https://doi.org/10.1128/JVI.01522-19
Liu L, Wang D, Mi S, Duan Z, Yang S, Song J, Xu G, Yang N, Yu Y. The different effects of viral and bacterial mimics maternal stimuli on ethology of hens and reproduction of their offspring. Poult Sci. 2019 98:4153-4160 doi: 10.3382/ps/pez189
Losada-Medina D, Yitbarek A, Uribe-Diaz S, Ahmed M and Rodríguez-Lecompte JC. 2020. Identification, tissue characterization and innate immune role of Angiogenin-4 expression in young broiler chickens. Poult. Sci. 99:2992-3000
Mon, K.Z., Y. Zhu, G. Chanthavixay, C. Kern and H. Zhou. 2020. Integrative analysis of gut microbiome and metabolites revealed novel mechanisms of intestinal Salmonella carriage in chicken. Sci Rep. 10:4809. www.nature.com/articles/s41598-020-60892-9
Mushi, G. Chiwanga, E. Amuzu-Aweh, M. Walugembe, R. Max, S.J. Lamont, T. Kelly, E. Mollel, P. Msoffe, J. Dekkers, R. A. Gallardo, H. Zhou, A. Muhairwa. Phenotypic variability and population structure analysis of Tanzanian free range local chickens. BMC Veterinary Research 16, 360 (2020). https://doi.org/10.1186/s12917-020-02541-x
Neerukonda SN, Katneni UK, Bhandari N, Parcells MS. Transcriptional Analyses of Innate and Acquired Immune Patterning Elicited by Marek's Disease Virus Vaccine Strains: Turkey Herpesvirus (HVT), Marek's Disease Virus 2 (strain SB1), and Bivalent Vaccines (HVT/SB1 and HVT-LT/SB1). Avian Dis. 2019 Dec;63(4):670-680. doi: 10.1637/aviandiseases-D-19-00117. PMID: 31865682.
Oxford, J. H., & Selvaraj, R. K. (n.d.). Effects of Glutamine Supplementation on Broiler Performance and Intestinal Immune Parameters During an Experimental Coccidiosis Infection. The Journal of Applied Poultry Research. doi:10.3382/japr/pfz095
Perry, F., Johnson, C.N., Aylward, B.A., Arsenault, R.J. The Differential Phosphorylation-Dependent Signaling and Glucose Immunometabolic Responses Induced during Infection by Salmonella Enteritidis and Salmonella Heidelberg in Chicken Macrophage-like cells. 2020. Microorganisms. 8(7), 1041.
Ponnuraj, N.P., Y.T. Tien, W. Vega Rodriguez, A. Krieter, and K.W. Jarosinski. 2019. The Herpesviridae conserved multifunctional infected cell protein 27 (ICP27) is important, but not required for replication and oncogenicity of Marek’s disease alphaherpesvirus (MDV). J Virol. 93(4); e01903-18. https://doi.org/10.1128/jvi.01903-18
Redweik, G.A.J., Kogut, M.H., Arsenault, R.J., and Mellata, M. Oral Treatment with Ileal Spores Triggers Immunometabolic Shifts in Chicken Gut. 2020. Frontiers in Veterinary Science. 7 (629) https://doi.org/10.3389/fvets.2020.00629.
Selvaraj, R., Markazi, A., Shanmugasundaram, R., Murugesan, R., & Mohnl, M. (2019). Effects of acidifier supplementation in laying hens challenged with Salmonella. Journal of Applied Poultry Research, 28, 919-929
Selvaraj, R., Renu, S., Markazi, A., dhakal, S., Shanmugasundaram, R., & Gourapura, R. (2019). Oral deliverable mucoadhesive chitosan-Salmonella subunit nanovaccine for layer chickens. International Journal of Nanomedicine 2020; 15: 761–777
Selvaraj, R., Shanmugasundaram, R., Markazi, A., Mortada, M., Bielke, L., Applegate, T., & Murugesan, R. Effect of Synbiotic Supplementation on Caecal Clostridium perfringens load in Broiler Chickens with different Necrotic Enteritis Challenge Models. Poult. Sci. 99, 2020, 2452-2458
Shanmugasundaram, R., Morris, A., & Selvaraj, R. (2019). Effect of 25-hydroxycholecalciferol supplementation on turkey performance and immune cell parameters in a coccidial infection model. Poultry Science, 98, 1127-1133. Retrieved from http://dx.doi.org/10.3382/ps/pey480
Shanmugasundaram, R., Mortada, M., Cosby, D. E., Singh, M., Applegate, T. J., Syed, B., Selvaraj, R. K. (2019). Synbiotic supplementation to decrease Salmonella colonization in the intestine and carcass contamination in broiler birds.. PloS one, 14(10), e0223577. doi:10.1371/journal.pone.0223577
Swaggerty,C.L., Arsenault, R.J., Johnson, C., Piva, A., and Grilli, E. Dietary supplementation with a microencapsulated blend of organic acids and botanicals alters the kinome in the ileum and jejunum to enhance growth and feed efficiency in broilers. 2020. PLOS One. 15(7), e0236950.
Talghari M, Behnamifar A, Rahimi S, Torshizi MAK, Beckstead RB, Grimes JL. The effect of sodium bisulfate and coccidiostat on intestinal lesions and growth performance of Eimeria spp. challenged broilers. Poultry Science. 99:4769-4775
Taylor, R. L., Jr. 2020. A Year of Change. Poult. Sci. 99:6291–6292 https://doi.org/10.1016/j.psj.2020.09.018
Uribe-Diaz S, A. Yitbarek A, Vallejo D, Losada-Medina D, Ahmed M and Rodríguez-Lecompte 2020. Effect of folic acid on the innate immune receptors in chicken B cells infected with IBDV. Veterinary Immunology and Immunopathology in press
Vega-Rodriguez, W., Ponnuraj, N.P., and K.W. Jarosinski. 2019. Marek’s disease alphaherpesvirus (MDV) RLORF4 is not required for expression of glycoprotein C and interindividual spread. Virology 534:108-113. https://doi.org/10.1016/j.virol.2019.06.008
Walugembe, M., Amuzu-Aweh,E.N., Botchway, P.K., Naazie, A., Aning, G., Wang, Y., Saelao, P., Kelly, T., Gallardo, R.A., Zhou, H., Lamont, S.J., Kayang, B., Dekkers, J. 2020. Genetic Basis of Response of Ghanaian Local Chickens to Infection with a Lentogenic Newcastle Disease Virus, Frontiers Genetics https://doi.org/10.3389/fgene.2020.00739
Wang, Y., Saelao, P., Kern, C., Jin, S., Gallardo, R.A., Kelly, T., Dekkers, J.M., Lamont, S.J. and Zhou, H., 2020. Liver Transcriptome Responses to Heat Stress and Newcastle Disease Virus Infection in Genetically Distinct Chicken Inbred Lines. Genes, 11(9), p.1067.
Wang, Y., Saelao, P., Kern, C., Jin, S., Gallardo, R.A., Kelly, T., Dekkers, J.M., Lamont, S.J., Zhou, H. 2020. Liver transcriptome responses to heat stress and Newcastle Disease virus infection in genetically distinct chicken inbred lines. Genes 11, 1067; doi:10.3390/genes11091067
Ward, T.L., Weber, B.P., Mendoza, K.M., Danzeisen, J.L., Llop, K., Lang, K., Clayton, J.B., Grace, E., Brannon, J., Radovic, I., Beauclaire, M., Heisel, T.J., Knights, D., Cardona, C., Kogut, M., Johnson, C., Noll, S.L., Arsenault, R., Reed, K.M., and Johnson, T.J. Antibiotics and host-tailored probiotics similarly modulate effects on the developing avian microbiome, mycobiome, and host gene expression. 2019. mBio. 10(5), 02171-19
Wilkinson, N. G., R. T. Kopulos, L. M. Yates, W. E. Briles, and R. L. Taylor, Jr. 2020. Major histocompatibility (B) complex recombinant R13 antibody response against bovine red blood cells. Poult. Sci. 99:4804-4808 https://doi.org/10.1016/j.psj.2020.06.069
You Z, Zhang Q, Liu C, Song J, Yang N, Lian L. Integrated analysis of lncRNA and mRNA repertoires in Marek's disease infected spleens identifies genes relevant to resistance. BMC Genomics. 2019 Mar 28;20(1):245. doi: 10.1186/s12864-019-5625-1.
Zhang, J. R. M. Goto and M. M. Miller. 2020. A simple means for MHC-Y genotyping in chickens using short tandem repeat sequences. Immunogenetics 72(5):325-332. doi: 10.1007/s00251-020-01166-6.
Zhang, J., Kaiser, M. G., Gallardo, R. A., Kelly, T. R., Dekkers, J., Zhou, H., & Lamont, S. J. (2020). Transcriptome analysis reveals inhibitory effects of lentogenic Newcastle disease virus on cell survival and immune function in spleen of commercial layer chicks. Genes, 11(9), 1003.
Zhang, J., Kaiser, M.G., Gallardo, R.A., Kelly, T. R., Dekkers, J.C.M., Zhou, H., Lamont, S.J. 2020. Transcriptome analysis reveals inhibitory effects of lentogenic Newcastle disease virus on cell survival and immune function in spleen of commercial layer chicks. Genes 11:1003; doi:10.3390/genes11091003
Non-peer reviewed, rapid communication
Quinlan, B. D., H. Mou, L. Zhang, Y. Guo, W. He, A. Ojha, M. S. Parcells, G. Luo, W. Li, G. Zhong, H. Choe, and M. Farzan,, The SARS-CoV-2 Receptor-Binding Domain Elicits a Potent Neutralizing Response Without Antibody-Dependent Enhancement. doi: https://doi.org/10.1101/2020.04.10.036418
Published Abstracts
Arsenault, R.J. Immunometabolism: The Potential Cause of and Solution to our Most Pressing Poultry Problems in Health and Infectious Disease. Proceedings of the 2020 Animal Nutrition Conference of Canada. 2020. Animal Nutrition Association of Canada, Ottawa, Canada, pp 68-74
Arsenault, R.J. The Immunometabolic Responses of Heritage and Modern Broilers to Immune Challenge: Learning From the Past to Inform the Future. Symposium on Gut Health in Production of Food Animals; 2019 November 4-6; St. Louis, MO.
Boothe SM, Emami NK, White MB, Dalloul RA. In-house hatching and early feeding to improve chick performance during a necrotic enteritis challenge. International Poultry Scientific Forum (IPSF), SPSS and SCAD. Atlanta, GA. 2020.
Çalik A, Emami NK, White MB, Dalloul RA. The impact of dietary vitamin E and selenium on body composition, intestinal integrity and immunity of broilers subjected to heat stress. International Poultry Scientific Forum (IPSF), SPSS and SCAD. Atlanta, GA. 2020.
Chadwick, Elle Jesse Grimes, Shaban Rahimi, John Pitts, and Robert Beckstead Sodium bisulfate aids broilers in growth and intestinal health during a coccidiosis challenge. International Poultry Scientific Forum (IPSF), SPSS and SCAD. Atlanta, GA. 2020
Chadwick, Elle, Robert Beckstead. Dietary additives, coccidiosis and Fenbendazole treatment alter fecal moisture to various degrees in turkey poults. Poultry Science Association Annual Meeting, 2020
Chasser, Kaylin, Audrey F. Duff, Whitney Briggs, Kate McGovern, Johel Bielke, Lisa Bielke. Day of hatch exposure to Enterobacteriaceae and characterization of avian pathogenic E. coli on inflammation. Poultry Science Association Virtual Conference, July 2020.
Chasser, Kaylin, Kate McGovern, Audrey F. Duff, B D. Graham, Whitney Briggs, Denise Russi Rodrigues, Johel Bielke, and Lisa Bielke. Effect of day of hatch inoculation with Gram-negative bacteria on gastrointestinal inflammation. International Poultry Scientific Forum (IPSF), SPSS and SCAD. Atlanta, GA. 2020.
Cox, J. L., D. R. Rodrigues, A. F. Duff, K. M. Chasser, J. C. Bielke, D. Jeffrey, C. Risch, D. Shafer, L. R. Bielke. Characterization of intestinal microbiota of newly hatched ducklings. International Poultry Scientific Forum (IPSF), SPSS and SCAD. Atlanta, GA. 2020.
Cueva, Justin R., Nicholas Egan, Imane Assakhi, Phaedra Tavlarides-Hontz, and Mark S. Parcells. Sequential Interactions of Meq Proteins with Polycomb Repressive Complex Proteins in Marek’s Disease Virus Latency. Proceedings of the 92nd NECAD, Penn State University, September 15, 2020
Cupo, Katherine Catherine Fudge, Kelly Keen, and Robert Beckstead Development of a diagnostic PCR specific to the Cochlosoma anatis 28S ribosomal gene. International Poultry Scientific Forum (IPSF), SPSS and SCAD. Atlanta, GA. 2020
Dallakoti, Aksana, Eric Munoz, Matthew D. Huson, Shannon Modla and Mark S. Parcells. The Role of Exosomes in Marek’s Disease Virus Vaccine Responses. Proceedings of the 92nd NECAD, Penn State University, September 15, 2020
Drechsler Y, and Hawkins D. 2020. Infectious Bronchitis Virus Infection Affects Chromatin Accessibility and RNA Differential Expression in a Tissue-Specific Manner. Plant & Animal Genome XXVIII, San Diego, CA.
Duff, Audrey F., K. M. Chasser, W. N. Briggs, D. Russi-Rodrigues, L. R. Bielke. Age-Related Changes in Gut Permeability and Optimal Timing for Experimentally Induced Leaky Gut. International Poultry Scientific Forum (IPSF), SPSS and SCAD. Atlanta, GA. 2020.
Duff, Audrey F., Kaylin Chasser, Whitney Briggs, Johel Bielke, Shelby Ramirez, Antonia Tacconi, G. R. Murugesan, Lisa Bielke. Necrotic enteritis model to achieve mortality reflective of industry. Poultry Science Association Virtual Conference, July 2020
Emami NK, Boothe SM, White MB, Dalloul RA. Effects of a naturally occurring necrotic enteritis challenge on performance, lesion scores, and expression of tight junction proteins in broiler chickens. International Poultry Scientific Forum (IPSF), SPSS and SCAD. Atlanta, GA. 2020.
Erf, G. F., G. Le Pape, S. Rémy, and C. Denesvre. 2020. Vitiligo onset favors earlier HVT replication in feathers of Smyth chickens. 13th International Symposium on Marek’s disease and avian herpesviruses, June 14-17, 2020, Guelph, Ontario, Canada (in press).
Gogineni, Vivek, Huawei Wang, Kyle Moskowitz, Prasad Dhurjati, Joshua Miller, Benedikt Kaufer, and Mark S. Parcells. Agent-based Modeling of Marek’s Disease Virus Lytic Infection. 92nd Annual Northeastern Conference on Avian Diseases (NECAD) Penn State, virtual, Sept. 15 and 16, 2020.
Hawkins D. and Drechsler Y. 2020. Epigenomic annotation of candidate cis-regulatory elements in the chicken genome. Plant & Animal Genome XXVIII, San Diego, CA.
Koci, M. D., A. Ballou, X. Wei, L. Zhang, Z. Q. Liew, and R. Ali. 2020. Connecting the microbiome to host metabolites: understanding how the microbiome controls immune activity in birds. Experimental Biology 2020. San Diego, CA.
McGovern, Kate, K.M.Chasser, A.F.Duff, W.N.Briggs, D.R. Rodrigues, L.R.Bielke. Effect of Select Gram Negative Bacteria on Alpha-1-Acid-Glycoprotein and Ileal Histology. International Poultry Scientific Forum (IPSF), SPSS and SCAD. Atlanta, GA. 2020.
Miller, Joshua, Kyle Moskowitz, Samuel Keating, Abhyudai Singh, Prasad Dhurjati, Andelé Conradie, Benedikt B. Kaufer, Phaedra Travlarides-Hontz, Mark Parcells. The Development of Agent-based and Mathematical Models for Marek’s disease virus (MDV) Lytic and Latent Infections. Proceedings of the 92nd NECAD, Penn State University, September 15, 2020
Miller, M. M., J. Zhang, R. M. Goto, C. F. Honaker, P. B. Siegel, R. L. Taylor, Jr., and H. K. Parmentier. 2020. Major advances in defining variability and function of chicken MHC-Y region genes. PAG XXVII https://plan.core-apps.com/pag_2020/abstract/66376fdb-aa85-4703-a8d0-b3a4372e75f2
Patria, Joseph, Nirajan Bhandari, Phaedra Travlarides-Hontz, Andelé Conradie, Benedikt B. Kaufer, and Mark S. Parcells. Mutations in the Meq oncoprotein of MDV may be selected based on innate immune and latent T-cell interactomes. Proceedings of the 92nd NECAD, Penn State University, September 15, 2020.
Rodrigues, D. R., J. L. Cox, A. Hysong, W. Briggs, A. Duff, K. Chasser, J. Bielke, K. Wilson, C. Risch, D. Jeffrey, D. Shafer, and L.Bielke. Strategies to manipulate early-life microbiota of poultry toward beneficial bacterial growth. International Poultry Scientific Forum (IPSF), SPSS and SCAD. Atlanta, GA. 2020.
Rodríguez-Lecompte JC, Jaime J, Vargas-Bermúdez DS, Yitbarek A , and Reyes J. 2019. Susceptibility and characterization of anti-viral innate immune responses in chicken B cells infected with infectious bursa diseases virus and supplemented with 1,25(OH)2 D3. AVMA Convention-AAAP, Washington, DC, August 2-6, 2019.
Rodríguez-Lecompte JC, Jaime J, Vargas-Bermúdez DS, Yitbarek A , and Reyes J. 2019. Effect of 1,25(OH)2 D3 on gene mRNA expression of innate immune toll-like receptors, proteins signal adapters, RIGI-like receptor, IFN type I, IFN-induced proteins, and proinflammatory cytokines on chicken fibroblast infected with IBDV. The Western Poultry Disease Conference-Association of Poultry Science Specialists (ANECA). Puerto Vallarta, Mexico, April 2-6, 2019.
Rodríguez-Lecompte JC, Jaime J, Vargas-Bermúdez DS, YitbarekA , and Reyes J. 2019. Chicken macrophages’ susceptibility and innate immune response to infectious bursal disease virus with the supplementation of 1,25(OH)2 D3. International Poultry Scientific Forum (IPSF), SPSS and SCAD. Atlanta, GA, February 11-14, 2019.
Rodríguez-Lecompte JC, Uribe-Diaz S, Martinez-Morales BC, Despres B, and J. Reyes. 2019. Pre- and post-challenge effects of essential oils (EO) on mRNA expression of antiviral and pro-inflammatory pathways genes on chicken macrophages infected with IBDV. International Poultry Scientific Forum (IPSF), SPSS and SCAD. Atlanta, GA, January 28-30, 2020.
Sigmon, Christina S., Alessandro Ferrarini, Robert Beckstead. The use of a direct ELISA to identify Blackhead resistant turkeys. Poultry Science Association Annual Meeting, 2020
Taylor, R. L., Jr., W. Drobik-Czwarno, and J. E. Fulton. 2020. Candidate gene for chicken alloantigen A. Poult. Sci. 99(E-Suppl. 1):45-46
Tracy, K., R. A. Gallardo, E. Aston, and H. Zhou. 2020. Role of Humoral Immunity in Clearance of Lentogenic Newcastle Disease Virus in Chickens. Plant & Animal Genome XXVIII, San Diego, CA.
Uribe-Diaz S, Pirateque JF, Despres B, Reyes J, and Rodríguez-Lecompte JC. 2019. Effect of essential oils (EO) on mRNA expression of antiviral and pro-inflammatory innate immunity genes on chicken macrophages infected with IBDV. PSA annual meeting. Montreal, QC, Canada, July 15-18, 2019.
Uribe-Diaz S, Yitbarek A, Vallejo D, Losada-Medina D, Ahmed M, and Rodríguez-Lecompte JC. 2020. Role of folic acid on the antiviral innate immune pathways in chicken B-lymphocytes infected with IBDV. PSA annual meeting. Online, July 15-18, 2020.
Uribe-Diaz S, Yitbarek A, Vallejo D, Losada-Medina D, Ahmed M, and Rodríguez-Lecompte JC. 2019. Effect of folic acid on the innate immune receptors in chicken B-lymphocytes infected with IBDV. International Poultry Scientific Forum (IPSF), SPSS and SCAD. Atlanta, GA, January 28-30, 2020.
Vignale-Pollock, Karen, Elle Chadwick, and Robert Beckstead. The effect of feeding encapsulated butyric acid and zinc on disease signs of turkeys challenged with histomonas meleagridis and coccidia. International Poultry Scientific Forum (IPSF), SPSS and SCAD. Atlanta, GA. 2020.
Wang Y., Chanthavixay K., C. Kern, Saelao, P., R. Gallardo, S.J. Lamont. N. Chubb, G. Rincon, Zhou, H. 2020. Identification of Active Promoters and Enhancers By H3K27ac Peaks in the Spleen Tissue of Two Inbred Chicken Lines Under NDV Infection and Heat Stress. Plant & Animal Genome XXVIII, San Diego, CA.
White MB, Boothe SM, Dalloul RA. Broiler response to a necrotic enteritis challenge after varying in ovo doses of commercial probiotics. International Poultry Scientific Forum (IPSF), SPSS and SCAD. Atlanta, GA. 2020.
Wisser-Parker, Kristy, Kristen Wooten, Phaedra Talverides-Hontz, and Mark S. Parcells. Assessment of the Role of IRG1 and Itaconate on Marek’s diseases virus (MDV) Infection. Proceedings of the 92nd NECAD, Penn State University, September 15, 2020
Undergraduate Research Symposium:
Gogineni, Vivek, Huawei Wang, Kyle Moskowitz, Prasad Dhurjati, Joshua Miller, Benedikt Kaufer, and Mark S. Parcells. Agent-based Modeling of Marek’s Disease Virus Infection of Chicken Embryo Fibroblasts (CEF). University of Delaware Undergraduate Research Symposium August 13, 2020
Wang, Huawei, Vivek Gogineni, Kyle Moskowitz, Prasad Dhurjati, Joshua Miller, Benedikt Kaufer, and Mark S. Parcells. Agent-based Modeling of Marek’s Disease Virus Reactivation from Latency. University of Delaware Undergraduate Research Symposium August 13, 2020
Book Chapters:
Cheng, H.H. and Lamont, S.J. 2020. Genetics of disease resistance. pp. 90-108. In: Diseases of Poultry. 14th ed. D.E. Swayne, M. Boulianne, C.M. Logue, L.R. McDougald, V. Nair, and D.L. Suarez, Eds. Wiley-Blackwell, Hoboken
Erf, G. F. Autoimmune diseases of poultry. in: Avian Immunology, Schat, K. A., Kaspers B. and, P. Kaiser, editors. Elsevier, Academic Press, San Diego, CA. 3rd edition of Avian Immunology in press.
Klasing, K.C., and D.R. Korver. 2020. Nutritional diseases. pp. 1255-1285. In: Diseases of Poultry. 14th ed. D.E. Swayne, M. Boulianne, C.M. Logue, L.R. McDougald, V. Nair, and D.L. Suarez, Eds. Wiley-Blackwell, Hoboken
Pinard-van der Laan, M.-H., J. Kaufman, A. Psifidi, H. Zhou, M. Fife. 2020. Genetics and genomics of immunity and disease traits in poultry species. In S. E. Aggrey, H. Zhou, M. Tixier-Boichard and D. D. Rhoads (Eds). 2020 Advances in poultry genetics and genomics. Burleigh Dodds Science Publishing ISBN:978-1-78676-324.
6.3 Thesis/Dissertation
Aylward, Bridget. A Comparative Evaluation of the Gastrointestinal Immune Response of the Modern and Heritage Broiler Chicken. Ph.D. Dissertation. 2020. University of Delaware. Supervisor: Ryan J. Arsenault
Boothe, Siobhan M. In-house hatching and early feeding to improve chick performance during a necrotic enteritis challenge. MS Thesis, Virginia Tech. Supervisor: Rami A. Dalloul
Briggs, Whitney. Evaluation and optimization of quantitative analysis methods for C. perfringens detection in broiler intestinal samples to use with necrotic enteritis challenge models. M. S. thesis, 2020. Ohio State University. Supervisor: Lisa R. Bielke
Chadwick, Elle. The role of poultry parasites in gut health and production. PhD Dissertation, 2020. North Carolina State University. Supervisor: Robert B. Beckstead
Cox, Jeremiah. Characterization of intestinal microbiota of newly hatched ducklings. Undergraduate research thesis, 2020. Ohio State University. Supervisor: Lisa R. Bielke https://kb.osu.edu/handle/1811/91796
Ellington, C. Effects of dietary copper, zinc and manganese source and level on the acute inflammatory response of broilers. MS Thesis, 2019. University of Arkansas, Fayetteville Supervisor: G. F. Erf
Tracy, Karen. The systemic immune response to Newcastle disease virus infection in chickens. PhD Dissertation, 2020. University of California-Davis. Supervisor: Rodrigo A. Gallardo
Emami, Nima K. Managing poultry gut integrity, immunity and microbial balance during necrotic enteritis. PhD Dissertation. 2020 Virginia Tech. Supervisor: Rami A. Dalloul