Brief Summary of Minutes of Annual Meeting

Members briefly introduced / reacquainted themselves.

Administrative updates (Steve Lommel and Hongda Chen) relayed on-going operational challenges, including severe budgetary cuts at experiment stations in upcoming year, move of USDA-NIFA to Kansas City and ensuing loss of 80% of staff. Nevertheless USDA has invested $16.1 M in competitive research programs relevant to this project, including Foundational and Applied Science priority area A1511 (Nanotechnology for Agricultural and Food Systems) relevant to this project at funding rates of ~$5M in 2017 and 2018. Hongda Chen also promoted the Rapid Response to COVID-19 solicitation ($9 M) with proposals due June 4, 2020.

Administratively projects are asked for more abbreviated (3 page limit) annual reports with stronger emphasis on impacts and products and alignment to societal challenges. Also this project is coming into its final year, so members will need to submit a renewal proposal addressing new directions after reflecting on the team’s expertise, developed collaborations among the members and how these collaborations stimulate new grants that create new avenues for research and training.

Station reports were delivered from each participant in the meeting describing completing and on-going research.

Jose Reyes-de-Corcuera was elected Secretary of the project for the coming year (with Olga Tsyusko advancing to vice-chair, and Daniel Jenkins advancing to chair). The remainder of the meeting was devoted to discussing options for future meetings and coordinating joint projects, publications, and collaborations. Full minutes are available at []

Over this project year participants made numerous contributions towards achieving the corresponding (year 4) milestones delineated in our original proposal.

1. Continue to validate the biosensors and other devices in real matrices.

Participants reported numerous advances in nanomaterial-enabled biosensor technologies across a spectrum of applications, and to novel antimicrobial properties of nanomaterials to improve safety and security of the food supply. Notable advances include innovative nanoparticle morphologies for enhanced Surface Enhanced Raman Scattering (SERS) for pesticide analysis (MO), investigating metabolite distributions in plant and animal cells (IA, UT), and to enhance performance of a variety of other biosensing modalities including for microbial and chemical contaminants in food and water (AR, AZ, FL, HI, IA, MI, WI). Participants deepened multidisciplinary collaborations to address antimicrobial resistance, advancing affordable technologies for rapid clinical identification of antimicrobial resistance in pathogenic bacteria (MI), and novel approaches to preventing and treating infections with pathogenic organisms demonstrated successfully in animal models (SC). To support commercialization of new technologies participants contributed to the development of open source smart-phone based approaches for portable analysis (AR, AZ, HI), scalable disposable sensors including using paper microfluidics (AZ), scalably manufactured graphene (FL, IA), as well as stabilization of immobilized biomaterials (GA). At least two groups have made significant progress characterizing and understanding mechanisms of toxicology of a wide array of commonly used nanomaterials (KY, WI). Participants have also engineered new nanomaterials for targeted drug delivery (AR, IA) or vaccines against animal viruses (VA), to make edible films with novel antimicrobial and nutritional properties (NJ), to improve efficiency / rates for value-added bioprocessing (AR, WI), and enhance speed and sensitivity for recovery and detection of pathogenic bacteria (AR, MI). Complementary to work discrete biosensing technologies for wide distribution in the environment, several participants of this project are also working on networking approaches and artificial intelligence to aggregate data and provide meaningful decision support to improve food quality, safety, and the cost-effectiveness of agriculture and bioprocessing (AR, AZ, FL, MI, NJ).

2. Identify potential industry partners and initiate meetings with these partners, and;
3. Assess market-readiness of the technologies.

Several groups partnered in various initiatives to strengthen ties to industry, including identification and execution of new research needs, and to strengthen research capacity globally to develop practical, cost-effective biosensing technologies. These include partnerships with the Global Alliance for Rapid Diagnostics (GARD) to establish centers of excellence (COEs) around the world, and agreements with food companies to validate and license technologies for rapid pathogen extraction / detection (MI). Walmart foundation awarded $3.5 M to a group lead by a project member from Arkansas for research on biosensors for food safety. A consortium of University groups including participants in this project (FL, IA) are actively involved in a funded planning grant under NSF-IUCRC (Industry-University Cooperative Research Center) to use nano-enabled sensory tools to study soil dynamics, and technologies developed by members of our group have successfully been used by government and industry, such as handheld biosensors for bacteria in drinking water used by Tucson Water (AZ). Participants at Florida are working with SBIR funding to develop new mobile device APIs to facilitate risk analysis in water quality.

4. Continue the exchange of educational teaching materials on nanotechnology and biosensors among member institutions.

Project members are highly active in instruction, developing and sharing teaching / training materials related to nanotechnology and biosensors. This includes leading two NSF-REU programs (IA) where students contributed to research and development of wearable graphene-based stress sensors.

5. Conduct annual meeting to report progress of research activities.

In person reporting of progress from each station was made in an annual meeting virtually on May 21-22, 2020. Minutes of this meeting are at the link above.

Outputs of this project this year include at least 76 peer-reviewed publications in high impact journals, and research is regularly highlighted as the feature article in their respective issues, and / or covered in the scientific press (i.e. NSF Research News and CEP Magazine) (AZ). Participants have notably made publicly available several research tools to help identify relevant research (SENSEE; FL) and share protocols for research related to biosensors and antimicrobial resistance (IA, MI).

Activities planned for the upcoming year are to collaborate on a review paper on biosensors in food, agriculture, and the environment to help direct the focus of the group, enhance collaborations on interdisciplinary topics related to antimicrobial resistance, and to consider future directions to guide development of a renewal proposal for NC-1194 and to support successful transition of new technologies and ideas to industry.

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27. Jenkins, D.M., Lee, B.E., Jun, S., Reyes-De-Corcuera, J., McLamore, E.S., 2019. ABE-Stat, a Fully Open-Source and Versatile Wireless Potentiostat Project Including Electrochemical Impedance Spectroscopy. J. Electrochem. Soc. 166, B3056. https://doi.org/10.1149/2.0061909jes
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63. Uz, M., Jackson, K., Donta, M.S., Jung, J., Lentner, M.T., Hondred, J.A., Claussen, J.C., Mallapragada, S.K., 2019. Fabrication of High-resolution Graphene-based Flexible Electronics via Polymer Casting. Sci. Rep. 9, 10595. https://doi.org/10.1038/s41598-019-46978-z
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67. Xiong, X., He, B., Jiang, D., Dong, X., Koosis, A., Yu, C., Qi, H., 2019. Postmortem biochemical and textural changes in the Patinopecten yessoensis adductor muscle (PYAM) during iced storage. Int. J. Food Prop. 22, 1024–1034. https://doi.org/10.1080/10942912.2019.1625367
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