Brief Summary of Minutes of Annual Meeting

SHORT-TERM OUTCOMES:

CA: 2017 Example #1:  Observed differences in reaction to Pasteuria isolates and resistance genes in pepper may be used to differentiate races of Meloidogyne hapla, which will allow growers to plant inherently resistance crop species and varieties to preserve crop yield and grower profitability.

2018 Example #1:  Experiments utilizing poultry-based compost enhanced the survival of enteric pathogens in soil more than dairy-based compost. This information can be used for dairy farms to shift away from poultry compost to increase bovine health and allow for easier compliance in organic agriculture.

In 2019 CA, Anaerobic soil disinfestation (ASD) was highly effective against plant-parasitic nematodes if done in a defined protocol at the proper time of year. Limitations of time of the year application, and nematode species and orchard or vineyard age at the time of treatment were explored.

CT: 2017-19:  Determined that while multiple nematode antagonistic crops could be grown in a single season to reduce populations of lesion and root-knot nematodes, difficulties establishing crops at certain times of year can allow weeds, therefore a single well established antagonistic crop may do just as well or better.

2018-19:  Determined that Litchi tomato, Solanum sisymbriifolium, is an effective trap crop that has similar results to soil fumigation and can affect nematodes up to 45 cm from plants.

FL: Demonstrated that the most common nematicides used on golf course turf (fluopyram and abamectin) are largely ineffective for managing lance nematode.  This assists turfgrass managers from applying needless nematicides treatments.

HI: Field trials funded by WSARE and NRCS PIA verified the versatility of brown mustard as biofumigant cover crop to suppress plant-parasitic nematodes while improving soil nutrient enrichment; Whereas drenching soil with crustacean meal provided more efficient control against banana Panama wilt caused by Fusarium oxysporum f. sp. cubense and improved bacterial decomposition in the soil. 

IL: Preliminary work of survey nematode communities from organic grain cropping systems.

MI: 

Quintanilla: Nematode control strategies such as nematicides, composts, and cover crops have been evaluated for crops such as corn, soy, sugar beets, potatoes, vegetables (carrots), fruits, ornamentals (daylilies).  The information was extended through publications, grower talks, and news.

Bird: In a comparison or six orchard preparation cover crop systems, two years of Essex rape and pearl millet resulted in the highest and red clover the lowest mechanically harvested sweet cherry yields.  In a trial with compost and mulch, sweet cherry yields were highest when compost was applied to the bottom of the tree planting hole, in addition to surface applied mulch or compost. 

MN: Evaluated 53 soybean varieties for SCN resistance and the data have been provided for farmers’ use.

Observed long-term crop sequence effect on soil microbial community, plant-parasitic nematode population density, and nematode community; determined the relationship between the soil microbial and nematode communities with crop productivity in the US Midwest cropping production system.  The information is useful for developing strategies to enhance soybean and corn productivity.

NY: 2017 Example #1:  Observed differences in reaction to Pasteuria isolates and resistance genes in pepper may be used to differentiate races of Meloidogyne hapla, which will allow growers to plant inherently resistance crop species and varieites to preserve crop yield and grower profitability.

2018 Example #1:  Experiments utilizing poultry based compost enhanced the survival of enteric pathogens in soil more than dairy-based compost. This information can be used for dairy farms to shift away from poultry compost to increase bovine health and allow for easier compliance in organic agriculture.

TN: A virus in soybean cyst nematode was successfully transferred to the root-knot nematode (RKN) Meloidogyne incognita, where it appears to be transovarial and thus maintained indefinitely on a tomato host, which becomes systemically infected as well.

VT: Pilot test of anaerobic soil disinfestation in hoop house was effective at reducing inoculum load of Rhizoctonia solani for lettuce production. Field trials were recently funded by NE-SARE.

OUTPUTS:

CA: 10 extension presentations and one departmental seminar in 2019.

CT: One referred articles, 2 chapters, and 1 abstract.  Conducted a short course on plant parasitic nematodes at the Northeast Agribusiness and Crop Consultants Association Conference held in Syracuse NY (November 28 and 29, 35 participants), served on the Master’s Degree thesis committee for a graduate student at Central Connecticut State University, who conducted research on hatch of cyst nematodes as a part of her thesis project.  Dr. LaMondia conducted 159 nematode diagnostic samples and conducted testing as an APHIS certified pinewood nematode export testing facility.

As a part of diagnostic services, we confirmed the first report of beech leaf disease caused by the foliar nematode Litylenchus crenatae subsp. mccannii in southwestern Connecticut.  Educational programs will be directed to arborists in the state.

FL: 3 Refereed journal publications, 1 Book chapter, 1 Paper presented at Society of Nematologists meeting, 7 posters presented at scientific meetings.

HI: 

• Conference/Symposium Presentations:

• Waisen, P., K.-H. Wang, and Brent Sipes. 2019. Will enhancement of the biofumigation effect compromise the soil health benefits of brassica cover crops? Society of Nematologists 58th annual meeting, Raleigh, NC. July 7-10, 2019.
• Wang, K.-H., P. Waisen, and J. Silva. 2019. The relationship between soil-borne disease pressure and soil health indicators as affected by biofumigation. Society of Nematologists 58th annual meeting, Raleigh, NC. July 7-10, 2019.
• Kerr, N., G. Spinelli, and K.-H. Wang. 2019. Saving ‘Pisang Awak’ banana from Panama wilt using anaerobic soil disinfestation. 31st Annual CTAHR Student Research Symposium, University of Hawaii at Manoa, Honolulu, HI. April 15, 2019 (Abstract 19).
• Samis, F., K.-H. Wang, B. Sipes, and C. Chan. 2019. Enhanced ecosystem screenhouses: a comprehensive approach to cucumber crop protection in Hawaii. 31st Annual CTAHR Student Research Symposium, University of Hawaii at Manoa, Honolulu, HI. April 15, 2019 (Abstract 8).
• Waisen, P., K.-H. Wang, Zhiqiang Cheng, and Brent Sipes. 2019. Below ground battle: Does biofumigation have non-target impacts on soil health promoting free-living nematodes? 31st Annual CTAHR Student Research Symposium, University of Hawaii at Manoa, Honolulu, HI. April 15, 2019 (Abstract 32).

• Extramural grant funded this year related to NE1640:

• Wang, K.-H., J. Silva, and J. Hawkins. 2019-2021. Finding new friends for sunn hemp to revitalized degraded soil in the tropic. NRCS CIG PIA $75,000.

IL:  A poster presentation was given at the American Pathological Society annual meeting. Poster entitled “Occurrence of Soybean Cyst Nematode and Free Living Nematode Communities in Organic Certified Fields in Central Illinois”. Authors include: Jaeyeong Ham, Carmen M. Ugarte, Nathan E. Schroeder, and Glen L. Hartman.

MI: 

Quintanilla: Eight invited oral presentations, 12 posters, four extension publications, two websites, five media or news articles, thirty extension/outreach presentations and 18 grants obtained.

Bird: The George Bird 2018-19 output included three book chapters two abstracts and one poster as described below. In addition, he continued to serve on the SCN Coalition Work Group

MN:  Nine refereed journal articles and two abstracts were published.  

NY:

Conference proceedings (2)

Gorny, A., Hay, F. S., and Pethybridge, S. J. 2018. Reproductive fitness of Meloidogyne hapla on eleven potato cultivars. Proc. World Potato Congress, Lima, Peru. Pp. 131.

Gorny, A., Hay, F. S., and Pethybridge, S. J. 2018. Differential responses of potato cultivars grown in New York to Meloidogyne hapla. Proc. Int. Congr. Plant Pathol. Boston, MA. Phytopathology S1.26.

Extension presentations (1)

Gorny, A., Hay, F. S., and Pethybridge, S. J. 2018. Reproductive fitness of Meloidogyne hapla on eleven potato cultivars. Proc. New York State Agricultural Experiment Station Research Symposium, Geneva, NY.  Pp. 7.

Extension Articles (1)

Gorny, A., Hay, F. S., and Pethybridge, S. J. 2018. Summary of research on the interaction of triticale and red clover crops with the northern root-knot and lesion nematodes. Information prepared for Hofmann Farm, Springville, New York (on request). Pp. 2.

TN: Two book chapters, three refereed articles, three abstracts, five presentations, 35 diagnostic samples

VT: Four different presentations were given to various grower groups and scientific audiences.

ACTIVITIES

Objective 1. Develop and integrate management tactics for control of plant-parasitic nematodes including biological, cultural (such as rotation or cover crops and plant resistance), and chemical.

CA: In CA, greenhouse experiments are conducted to determine the host status of legumes and brassicaceae plant lines towards plant-parasitic nematodes of major damaging potential in nut tree crops, Pratylenchus vulnus and Mesocriconema xenoplax.

Field experiments for a winter cover crop period are established. Two cover crop treatments (one including brassica, the second Merced Rye) show promise in either reducing nematode population densities or improving plant growth.

CT: Experiments were conducted to utilize a series of lesion nematode-suppressive rotation crops in tilled or no-till systems to try to achieve multiple cycles of suppression within a single year.  Under good conditions for crop establishment and biofumigation the rotation sequence was successful, but poor conditions for crop establishment or biofumigation resulted in poor nematode management.

We conducted field microplot experiments to evaluate the effects of nematode-susceptible or nematode-resistant plants, Litchi tomato, eastern black nightshade, and a cultivated bare fallow on Globodera tabacum cyst nematode population density changes.  Litchi tomato was the most effective at reducing populations, effective at least 45 cm from plants.  G. tabacum may be useful as a substitute model for the quarantined pathogen Globodera pallida for conducting trap crop experiments with S. sisymbriifolium under field conditions.

FL: Evaluated experimental chemical and biological nematicides in 5 lab trials, 6 greenhouse trials, and 12 field trials.

HI: Three field trials were conducted in Hawaii to determine the best termination method of brassicacea cover crops for soil biofumigation against plant-parasitic nematodes. Results showed that this biofumigation protocol suppressed population densities of both Meloidogyne spp. and Rotylenchulus reniformis consistently.

MA: The following products were trialed for their efficacy against plant parasitic nematodes: Diatomaceous earth, Monterey Nematode Control (saponins from Quillaja saponaria) , Majestene (Burkholderia spp. strain A396), Todal (Abamectin) and Nimitz Pro (Fluensulfone). None of these products reduced the nematode population significantly but the Nimitz Pro treatments had lower populations of nematodes than the untreated control.

MI:

Quintanilla: Similar to 2018, our trials have evaluated tactics for control of plant parasitic nematodes. We have evaluated new and established nematicides, bio-nematicides (biological), compost and manures, crop rotations, plant resistance and cover crops. In carrots and potatoes our trials have included mainly nematicides and compost. One particular compost is effective in lab trials and in some of the field trials. In soybeans we are evaluating rotation with resistant varieties to prevent building of (Soybean Cyst Nematode) SCN resistance, manures, and seed treatments. From greater to less effectiveness in SCN our and others trials: Resistant varieties and rotation of resistant varieties, rotating with non-host, chicken manure, and seed treatments. In sugar beets, we have evaluated nematicides, seed treatments, and trap crops. In corn, we have completed a survey of nematodes in corn field in the state of Michigan. In fruits, we are testing different strategies for prevention of replant problem (apples (cover crops, rootstock, killing old trees with herbicide among others) and cherries (mulch with Dr. Bird)), and in ornamentals we are evaluating nematicides, fumigants, and cultural strategies to control Northern Root Knot nematodes in daylilies. We have gotten excellent results in many of our trials.  In addition, several three graduate students are being trained to become nematologists in our program, which means that progress should continue and increase.

Bird: The Soybean Cyst Nematode Coalition involving 22 states, and 8 corporations.  It was launched at the 2018 Commodity Classic and is designed to assist in the prevention of resistance to PI88788 derived varieties. Between March 1 and June 13, 2019, it facilitated 33 news articles resulting in 2,678,521 electronic impressions.    

MN:  Effect of cover crops on the soybean cyst nematode:  Experiment was established in Minnesota in 2019 to study the effect of oilseed cover crops pennycress planting dates on the soybean cyst nematode (SCN) population.

Effect of sequences of resistance sources and tillage on SCN virulence: A field experiment was initiated in 2003 to study how tillage and 11 different sequences of four cultivars impact population dynamics and virulence.  An SCN-susceptible and three resistant cultivars, R1, R2, and R3, derived from PI 88788, Peking, and PI 437654, respectively, were used. Compared with no-till, conventional tillage resulted in a faster increase of SCN virulence to Peking by R2, and the virulence to PI 88788 by R3. The SCN populations selected by R1 overcame the resistance in PI 88788 but not Peking and PI 437654.  R2 selected SCN populations that overcame the resistance in Peking, but not PI 88788 and PI 437654.  In contrast, R3 selected SCN populations that overcame both PI 88788 and Peking sources of resistance.  R1 in rotation with R2 or R3 had negative effect on Female Index on Peking.  Susceptible soybean reduced SCN virulence to Peking indicating that there was fitness cost of the Peking-virulent SCN type.

NY A study to develop species-specific qPCR to detect and quantify Meloidogyne hapla in soil was completed successfully, and published.    Intensive sampling in fields of potato variety Lamoka (1 field), Eva (2 fields) and Envol (3 fields) in New York failed to derive a relationship between pre-plant population density of M. hapla and yield/quality of potato cultivars Envol, Eva and Lamoka, suggesting that these varieties were relatively tolerant of root-knot nematode feeding.  Spatial patterns of root knot nematode in fields were analyzed by geostatistics and SADIE to provide a basis for the development of improved sampling protocols for pre-plant nematode population densities.

Work has begun this year on investigating OMRI listed products for the control of seedborne pests and pathogens of garlic, including bloat nematode (Ditylenchus dipsaci).  A nematode nursery comprising five varieties of garlic has been planted in fall 2019, to provide infested material for trials in subsequent years.  

TN: Six fiber/seed hemp cultivars and eight high-CBD cultivars, along with “Rutgers” tomato as a check, were tested in greenhouse experiments for their suitability as hosts for Meloidogyne incognita (MI). All fiber/seed types were good hosts for MI, with final RFmax values of more than 10. Of the eight CBD cultivars, 5 were good hosts (RFmax 10-40), two were moderate hosts (RFmax <10), and one was nearly immune (RF<0.5). By comparison, RF for tomato was 40-90x the initial inoculum.

Objective 2. Determine the ecological interactions between nematode populations, nematode communities, ecosystems and soil health.

CA: In CA, soil samples from multiple field experiments testing the concept of so-called orchard recycling were collected. In this strategy ground dried tree residues after removal of an old almond orchard are incorporated before replanting to almond. In greenhouse tests, these soils are examined for the effects of the wood amendments on plant-parasitic nematodes damaging on almond. Most soils that had low field population densities expressed suppressiveness in the greenhouse experiments again root lesion nematode but possibly not against ring nematode.

In one project, anaerobic soil disinfestation is tested in various experimental contexts to determine the method’s limitations. Sites infested with different nematode species and following different crops are used for this testing.

FL: Demonstrated that the foliar nematode Aphelenchoides besseyi causes considerable damage to chrysanthemum.

Identified yellow and purple nutsedge as alternative hosts for sting nematode (Belonolaimus longicaudatus) and grass root-knot nematode (Meloidogyne graminis).

Identified Javanese root-knot nematode (Meloidogyne javanica) as a pathogen on Giant Bamboo.

Described a new species of Hirschmanniella, H. dicksoni, parasitizing forage grasses in Florida.

Demonstrated that the grass root-knot nematode exhibits different symptoms on different forage grass species.

Demonstrated that certain composts can improve turfgrass tolerance to sting nematode.

Demonstrated that turfgrass nematicides can lead to changes in nematode and arthropod community structures.

HI: Canonical Correspondence Analysis depicted that abundance of bacterivorous nematodes or enrichment index were positively related to efficacy of biofumigation. Field trials in Hawaii using ‘Sod Buster’ oil radish as a cover crop in a 9-year no-till field followed by a corn planting improved soil physical properties (field capacity, soil organic matter, cooler soil temperature) and led to higher abundance of bacterivorous nematode abundance, and indigenous entomopathogenic nematode (Heterorhabditis spp. H1) compared to a conventional tilled bare ground system.

MA: In 1999, Rochester NY golf greens were sampled to see if there was a relationship between fumigation of the greens (5 years previous to the sampling), and the number of Meloidogyne naasi juveniles, and the number of juveniles infected by Pasteuria. Three fumigated greens were compared to 3 non-fumigated greens. The fumigated greens had 7.5 times more juveniles than the non-fumigated greens. Thirty six percent of the juveniles in the fumigated greens were encumbered by Pasteuria. In the non-fumigated greens, 63% were incumbered with Pasteuria. We came to the conclusion that fumigation killed off natural enemies and the root-knot population soared. The population in the non-fumigated greens appeared to be held back by Pasteuria. The same six greens were extensively sampled in 2018, Nineteen years after the first study. The non-fumigated greens had 2.5% more juveniles and only 12% were infected by Pasteuria; a complete turn-around.

MI:

Quintanilla: We identified free living nematodes (nematode communities) and other aspects of soil health in several of our trials. Our apple replant project that evaluated beneficial organisms including nematodes has been published.  Our work on compost effect on controlling potato early die and increasing soil health has been accepted for publication in Phytopathology Journal. Sunnhemp cover crop has resulted in greater number of beneficial organisms, and this work is in the process of producing a publication.

Bird: In a soil health research project, under potato early-die conditions in Michigan, the highest tuber yields were associated with season-long cold and stable thermal conditions, compared to hot or variable thermal conditions. 

MN: Field plots of long-term corn-soybean crop sequences were established in 1982 in Minnesota, USA: (i) five-year rotation of each crop such that both crops are in years 1, 2, 3, 4, and 5 of monoculture every year; (ii) annual rotation of each crop with both crops planted each year; (iii) continuous monoculture of each crop.  Samples of bulk soil, rhizosphere soil, rhizoplane soil, crop roots, and SCN cysts were collected in 2014-2016 to study crop sequences effect on fungal and bacterial communities associated with SCN using cultural methods as well as metabarcoding DNA sequence analysis. 

Fungi communities in soil: Total fungal alpha diversity in soil was greater under corn, but patterns of diversity and relative abundance of specific functional fungal guilds differed by crop, with more pathotrophs and nematophagous fungi proliferating under soybean and more saprotrophs and symbiotrophs proliferating under corn.  Soil density of the SCN was positively correlated with relative abundance and diversity of nematode-trapping fungi and with the relative abundance of many potential nematode egg parasites.  Soil phosphorus (P) varied significantly by crop sequence, with lower levels of P corresponding with relative abundance of Glomerales, Paraglomerales, and Sebacinales and higher levels of P corresponding with relative abundance of Mortierellales. 

Fungal communities in SCN cysts: A majority of fungi in cysts belonged to Ascomycota and Basidiomycota, but the presence of several early diverging fungal subphyla thought to be primarily plant and litter associated, including Mortierellomycotina and Glomeromycotina. Species richness of fungi in cysts varied by both crop rotation and season and was higher in early years of crop rotation and in fall at the end of the growing season. Ecological guilds of fungi containing an animal-pathogen lifestyle, as well as potential egg-parasitic taxa previously isolated from SCN eggs, increased at midseason. The animal pathogen guilds included known (e.g., Pochonia chlamydosporia) and new candidate biocontrol organisms.

Fungal communities in roots: The Root and rhizosphere fungal communities differed between corn and soybean. Natural antagonists of the SCN such as nematode-trapping fungi and nematode endoparasites increased in relative abundance in the rhizosphere and root endosphere, respectively, over continuous soybean monoculture. In contrast, arbuscular mycorrhizal and plant-pathogenic fungi, several of which were negatively correlated with corn yield, increased in relative abundance over continuous corn monoculture.

TN: Nematode communities were quantified over a 3-year period in research pasture fields (switchgrass, orchard grass or big bluestem) that were treated with varying levels of N,P or K, and/or overseeded with annual ryegrass in the late fall. Nematode numbers were not affected by treatments. Distribution of nematodes in general and densities of plant-parasitic nematodes in particular were dependent upon species of pasture grass and soil texture rather than fertilizer or overseeding treatments. 

Objective 3. Outreach and communication - Compile and present/publish guidance on nematode management and management effects on soil health for different crops under different conditions.

CA: The PI gave multiple extension presentations and one invited seminar on the management of plant-parasitic nematodes including chemical and biorational methods. He also taught graduate students.

CT: One referred articles, 2 chapters, and 1 abstract.  Conducted a short course on plant parasitic nematodes at the Northeast Agribusiness and Crop Consultants Association Conference held in Syracuse NY (November 28 and 29, 35 participants), served on the Master’s Degree thesis committee for a graduate student at Central Connecticut State University, who conducted research on hatch of cyst nematodes as a part of her thesis project.  Dr. LaMondia conducted 159 nematode diagnostic samples and conducted testing as an APHIS certified pinewood nematode export testing facility.

As a part of diagnostic services, we confirmed the first report of beech leaf disease caused by the foliar nematode Litylenchus crenatae subsp. mccannii in southwestern Connecticut.  Educational programs will be directed to arborists in the state.

FL: One new extension publication: Grass root-knot nematode, Meloidogyne graminis.

Provided education at 22 stakeholder events, in 5 states, to a combined audience of 1395.

Provided diagnosis on 5454 nematode samples submitted to the UF Nematode Assay Lab.

HI: Outreach and communication - Compile and present/publish guidance on nematode management and management effects on soil health for different crops under different conditions.  The extension outreach consisted of two extension publications and seven farmer education or field days.

IL: The Soil Health Institute has been working to establish measurements and standards for the assessment of soil health across the country. Ugarte attended the Soil Health Institute conference held in Albuquerque, New Mexico from August 1 to August 3, 2018 and presented a poster that outlined best practices to build metadata standards that could facilitate the use and interpretation of soil health indicators.

MA and RI: A presentation, January 4th on “Introduction to Nematodes” was given at the Michigan State Turfgrass Conference to about 250 golf course superintendents. January 24^th a presentation on “Management of Nematodes in Turfgrasses”, sponsored by Advanced Turf Solutions, about 75 in attendance. March 6^th, “The Biology of Nematodes” and the New England Regional Turfgrass Conference. The UMass Extension Nematology Lab processed about 200 soil samples, the majority of which came from golf courses.  The URI Turfgrass Diagnostic Laboratory processed approximately 150 soil samples for nematode analysis.  Each sample offered a teaching moment for the superintendent who received results, a fact sheet about nematodes in turfgrasses and written recommendations.

MI: 

Quintanilla: Extension is one of our greatest priorities and our work is very applied and seeks to meet the needs of growers.   This is exemplified by eight invited oral presentations, 12 posters, four extension publications, two websites, five media or news articles, thirty extension/outreach presentations and 18 grants obtained.  All with an applied and extension focus.  For crops that were in our trials (corn, soy, sugar beets, potatoes, vegetables (carrots), fruits, ornamentals (daylilies)). Growers have had information and reports given to them personally and in grower talks. Relationship with extension agents and commodity groups has been essential in these efforts. These outreach efforts have included information on plant parasitic nematode control and also increase of soil health.

 Bird:

1. The first NE-1640 sponsored nematode short course was held in Michigan on January 4, 2018.  The course will focus on turf grass nematodes.  Four of the five presenters (Wick, Mitkowski, Crow and Bird) are Members of the Technical Committee of NE-1640 (The Short Course was attended by circa 150 members of the national turf-grass industry).
2. The second NE-1640 Short Course was held in East Syracuse, New York on November 29, 2018, as part of the NE Crop Consultants Conference.  It was be jointly sponsored by the SCN Coalition and include the following Program:
1. Morning Session (Gary Bergstrom, Moderator)
2. Soybean Cyst Nematode: Risk, Management and the SCN Coalition, (Bird, Michigan State University).
3. Afternoon Session (Gary Bergstrom, Moderator)
4. Plant Parasitic Nematodes of NY, NJ, PA and New England (LaMondia, Chief Scientist, Connecticut Agricultural Experiment Station).
5. Cornell Plant Disease Diagnostic Clinic (Karen Snover-Clift, Cornell University).
6. Golden Nematode Eradication Success Story (Xiohong Wang, Cornell University).
7. Nematode Management Practices and Products (George Bird, Professor, Michigan State University).
8. The Short Course on agronomic crop nematodes was attended by circa 150 Members of the NE Crop Consultant Association.

TN: A paper was published in American Biology Teacher on nematodes that live in millipede intestines. Although these nematodes are not plant parasites, they are excellent examples of Nematoda to study in biology-related classes, due to their size and the visibility of their internal organs, often with a stereo microscope.

 ACOMPLISHED MILESTONES (2018):

 CA: Continue cover crop experiments. Currently one in open field planting, and three in orchard experiments are ongoing.

 CT: Continued and adjusted cover- and rotation-crop experimental designs based on previous results.

Conducted a short course on plant parasitic nematodes at the Northeast Agribusiness and Crop Consultants Association Conference held in Syracuse NY.

HI: Evaluate new nematicidal products for efficacy – PI in Hawaii evaluate fluopyram against plant-parasitic nematodes on vegetable cropping systems in Hawaii

Investigate the relationship between the microbial community, plant-parasitic nematodes, soil health, and crop productivity – We present Canonical Multivariate analysis in the biofumigation trials and long-term no-till cover cropping trials to examine relationships between soil health and crop productivity.

Conduct grower education, annual short course and webinar (6 client presentations, 2 short courses related to nematode and soil health management were presented to farmers in Hawaii).

MN:  Continue cover- and rotation-crop experiments, determine the relationship between the microbial community, plant-parasitic nematodes, soil health, and crop productivity, and publish results

MI:  Trial results have been published and extended to growers.  We plant to continue our work and answer questions that have been raised by our research and to meet the needs of Michigan growers.

Objective 3 milestones were accomplished for 2018 through the multi-state short courses described in Bird’s Objective 3 section. 

Multiple states: New nematicidal products were tested for efficacy and two collaborative, turfgrass nematology seminars were given (Michigan and Providence, RI)

PUBLICATIONS

Bernard, E. C.  2018.  Plant-parasitic nematodes in Alaska. Pp. 241-246 In: Subbotin, S.A. & Chitambar, J.J. (Eds.). Plant Parasitic Nematodes in Sustainable Agriculture in North America, Vol. 1. Springer, Cham, Switzerland.

Bernard, E. C.  2018.  Plant-parasitic nematodes of Tennessee and Kentucky. Pp. 305-325 In: Subbotin, S.A. & Chitambar, J.J. (Eds.). Plant Parasitic Nematodes in Sustainable Agriculture in North America, Vol. 2. Springer, Cham, Switzerland.

Bernard, E.C., S.M. Schaeffer, L.S. Taylor, G. Phillips and J.M. Welker. 2019. High Arctic nematode communities of northwest Greenland. Journal of Nematology 51 (in press).

Bintarti, A.F., Wilson, J., Quintanilla-Tornel, M., and Shade, A.   2019. Biogeography and diversity of multi-trophic root zone microbiomes in Michigan apple orchards: analysis of rootstock, scion, and growing region.  Phytobiomes Journal.  Submitted

Bird, G. G. Abawi and J. LaMondia. 2018. Plant Parasitic Nematodes of New York, New Jersey and Pennsylvania. Pp. 27-56 m(in) Plant Parasitic Nematodes in Sustainable Agriculture of North America, Vol. 2, S. Subbotin and J. Chitambar (eds) Subbotin and J. Chitambar (eds) Springer Nature, New York. 457 pp.

Bird, G., G. Tylka and I. Zasada. 2018. Role of Population Dynamics and Damage Thresholds in Cyst Nematode Management. pp. 101-127 (in) Cyst Nematodes, R. Perry, M. Moens and J. Jones (eds), CABI, New York.  

Bird, G. and F. Warner. 2018. Nematodes and Nematologists of Michigan. pp. 57-85 (in) Plant Parasitic Nematodes in Sustainable Agriculture of North America, Vol. 2, S. Subbotin and J. Chitambar (eds) Subbotin and J. Chitambar (eds) Springer Nature, New York.  457 pp.

Cole, E., Pu, J., Chung, H., and Quintanilla, M.  2020.  Impacts of manures and manure-based composts on root lesion nematodes and Verticillium dahliae in Michigan potatoes. Phytopathology.  Approved

Cole, E., Parrado, L., and Quintanilla, M.  2019.  Selecting Soil Amendments and Nematicides to Best Prevent Potato Early Dying Complex.  Potato Country Magazine.  Submitted

Dandurand L. M., I. A. Zasada, and J. A. LaMondia 2019. Effect of the trap crop, Solanum sisymbriifolium, on Globodera pallida, Globodera tabacum, and Globodera ellingtonae. Journal of Nematology 51: ISSN (Online) 2640-396X, DOI: 10.21307/jofnem-2019-030, Mar 2019.

Darling, E., Thapa, S., and Quintanilla, M.  2020.  Exploring Alternative Strategies for Nematode Management for the processing carrot (cv. Cupar).  Carrot Country Magazine.  Submitted

Dyrdahl-Young, R., Cole, E., Quintanilla Tornel, M., Weldon, R., & DiGennaro, P.   2020. Economic assessment of nematode biological control agents in a potato production model. Nematology, 1(aop), 1-9

Eberlein, C., Zhang, R., Adelati, A., Westphal, A. 2019. Do liquid digestates, by-products of bioenergy production, have nematode-suppressive potential?  Progressive Crop Consultant JCS Marketing Vol 4 (4):16-20.

Gorny, A. M., Wang, X., Hay, F. S., and Pethybridge, S. J. 2019. Development of a species-specific quantitative PCR for detection and quantification of Meloidogyne hapla using the 16D10 root-knot nematode effector gene. Plant Dis. 103:1902-1909. https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-09-18-1539-RE.

Grabau, Z. J., Bao, Y., Vetsch, J. A., and Chen, S. Y. 2019.  Swine manure application enriches the soil food web in corn and soybean production.  Journal of Nematology 51:DOI: 10.21307/jofnem-2019-014 .

Gu, M., and W. T. Crow.  2018.  Abamectin, thiophanate-methyl, and iprodione for management of sting nematode on golf turf.  Nematropica 48:38-44.

Haarith, D., Hu, W. M., Kim, D. G., Showalter, D. N., Chen, S. Y., and Bushley, K. E. 2019.  Culturable mycobiome of soya bean cyst nematode (Heterodera glycines) cysts from a long-term soya bean-corn rotation system is dominated by Fusarium.  Fungal Ecology 42: doi.org/10.1016/j.funeco.2019.08.001.

Hu, W., Kidane, E., Neher, D.A., and Chen, S. 2019. Field and greenhouse evaluations of soil suppressiveness to Heterodera glycines in the Midwest corn-soybean production systems. Journal of Nematology 51:e2019-32. doi.org/10.21307/jofnem-2019-032

Hu, W. M., Strom, N., Haarith, D., Chen, S. Y., and Bushley, K. E. 2019.  Mycobiome of cysts of the soybean cyst nematode under long term crop rotation.  Frontiers in Microbiology 10:article 2671. doi.org/10.3389/fmicb.2019.02671.

Huang, D., G. Yan, N. Gudmestad, J. Whitworth, K. Frost, C. Brown, W. Ye, P. Agudelo, W. Crow.  2018.  Molecular characterization and identification of stubby-root nematode species from multiple states in the United States.  Plant Disease 102:2101-2111.

LaMondia, J. A., R. L. Wick and N. A. Mitkowski. 2018.  Plant Parasitic Nematodes of New England – Connecticut, Massachusetts and Rhode Island.  Chapter 1, Pp. 1-25.  Plant Parasitic Nematodes in Sustainable Agriculture in North America Volume 2” edited by S. A. Subbotin and J. J. Chitambar, Springer

LaMondia, J. A. 2018. Rotation crops for management of Pratylenchus penetrans in Connecticut. Journal of Nematology 50:644.

Levene, B., and Quintanilla, M. 2020.  Muddy fields and rush to finish field work may move soybean cyst nematodes.  MSU Extension.  https://www.canr.msu.edu/news/muddy-fields-and-rush-to-finish-field-work-may-move-soybean-cyst-nematodes.

Levene, B., Groulx, B., Stewart, J., and Quintanilla, M.  2019.  Evaluation of oilseed radish cover crop, pre-plant application timing/rate and in-furrow pesticide applications for nematode management.  Michigan Sugarbeet REACh journal, 2019 Variety trial results.  https://www.michigansugar.com/growing-production/research-information/

Levene, B., Groulx, B., Stewart, J., and Quintanilla, M.  2019.  Evaluation in-furrow and/or foliar pesticide applications for nematode management.  Michigan Sugarbeet REACh journal, 2019 Variety trial results.  https://www.michigansugar.com/growing-production/research-information/

Levene, B., Groulx, B., Stewart, J., and Quintanilla, M.  2019.  Evaluation in-furrow Abamectin treatments at planting for nematode management.  Michigan Sugarbeet REACh journal, 2019 Variety trial results.  https://www.michigansugar.com/growing-production/research-information/

Ma, X., R.T. Robbins, E.C. Bernard, C.M. Holguin and P. Agudelo. 2019. Morphological and molecular characterisation of Hoplolaimus smokyensis n. sp. (Nematoda: Hoplolaimidae), a lance nematode from Great Smoky Mountains National Park, USA. Nematology 21(9): 923-935.

Neher, D.A., Nishanthan, T., Grabau, Z.J., and Chen, S.Y. 2019. Crop rotation and tillage affect nematode communities more than biocides in monoculture soybean. Applied Soil Ecology 140: 89-97. doi.org/10.1016/j.apsoil.2019.03.016

Neher, D.A., Cutler, A.J., Weicht, T.R., Sharma, M., and Millner, P.D. 2019. Composts of poultry litter or dairy manure differentially affect survival of enteric bacteria in fields with spinach. Journal of Applied Microbiology 126:1910-1922. doi.org/10.1111/jam.14268

Phillips, G., D. I. Yates, R. M. Shelley, P. Ortstadt, and E. C. Bernard. 2019. Laboratory inquiries utilizing millipedes to demonstrate commensal parasitism. American Biology Teacher 81: 278-283.

Phillips, G. and E.C. Bernard. 2019. Biodiversity of Thelastoma spp. in North American millipedes and continued efforts toward revision of the genus. Journal of Nematology 51 (in press).

Pothula, S. K., P. S. Grewal, R. M. Auge, A. M. Saxton, and E. C. Bernard. 2019. Meta-analysis of the influence of agricultural intensification and urbanization on nematode diversity. Journal of Nematology e2019-11/51: 1-17.

Quintanilla, M., Cole, E., Poley, K., and Wilson, J.  2019.  Fruit replant problem with a special emphasis on nematodes.  New York State Horticultural Society Fruit Quartely 27: 19-21

Ravelombola, W. S., Qin, J., Shi, A., Nice, L., Bao, Y., Lorenz, A., Orf, J. H., Young, N. D., and Chen, S. Y. 2019.  Genome-wide association study and genomic selection for soybean chlorophyll content associated with soybean cyst nematode tolerance.  BMC Genomics 20:Article 94. DOI: 10.1186/s12864-019-6275-z.

Strom, N., Hu, W. M., Harrith, D., Chen, S. Y., and Bushley, K. E. 2019.  Continuous monoculture shapes root and rhizosphere fungal communities of corn and soybean in soybean cyst nematode-infested soil.  Phytobiome Journal 3:300-314. doi.org/10.1094/PBIOMES-05-19-0024-R.

Strom, N., Hu, W. M., Harrith, D., Chen, S. Y., and Bushley, K. E. 2019.  Interactions between soil properties, fungal communities, the soybean cyst nematode, and crop yield under continuous corn and soybean monoculture.  Applied Soil Ecology doi.org/10.1016/j.apsoil.2019.103388.

Strom, N., Hu, W. M., Harrith, D., Chen, S. Y., and Bushley, K. E. 2019.  Corn and soybean host root endophytic fungi with toxicity towards the soybean cyst nematode.  Phytopathology doi.org/10.1094/PHYTO-07-19-0243-R.

Taylor, L.S., A.R. Mason, G. Phillips, E.C. Bernard and J.M. DeBruyn. 2019. Forensic ecology: A comparative analysis of nematode succession in soils impacted by animal and human decomposition. Journal of Nematology 51 (in press).

Waisen, P.*, K.-H. Wang and B. S. Sipes. 2019. Effect of spirotetramat (Movento®) on hatch, penetration and reproduction of Rotylenchulus reniformis. Nematropica 49: (in press).

Waldo, B. D., Z. J. Grabau, T. M. Mengistu, W. T. Crow.  2019.  Nematicide effects on non-target nematodes in bermudagrass. Journal of Nematology 51: DOI: 10.21307/jofnem-2019-009.

Wilson, J., Quintanilla, M., Shade, A., Einhorn, T., Sundin, G., and A. Irish-Brown.  2019. The apple replant field trial at the Clarksville Research Center. New York State Horticultural Society, Fruit Quarterly, Vol 27(4): in press