Brief Summary of Minutes of Annual Meeting

Summary of Minutes – Annual Meeting:

The annual NC1184 technical committee meeting was held on October 20 and 21, 2017, at the Straughn Professional Development Center located in Gainesville, Florida, on the University of Florida campus; it was hosted by Dr. Tracy Scheffler of the Department of Animal Science, Florida State University. On October 20^th, Dr. Geoff Dahl, Department Head of Animal Science, welcomed the group and shared information about the department and the university. The group then began with oral station reports. A lunch break (kindly funded by Dr. Dahl) was provided. After lunch, the group continued with more station reports. In the evening, the group met for dinner, which was hosted by Dr. Tracy Scheffler at the University of Florida Beef Teaching Unit. The following morning the group started with a conference call with Dr. Mark Mirando (USDA-NIFA), who outlined the current funding programs, budgets, and statistics on the number of proposals submitted annually and funding rates. Following a question and answer session with Dr. Mirando, the remaining oral station reports were given. Following the station reports, the group discussed where the meeting would be held next year and it was decided that Dr. Tracy Scheffler would send an email out to the whole group as there were relatively few attendees at this year’s meeting. After that email was sent, it was decided that Dr. Derris Burnett, of the Mississippi station, would host in 2019.

Accomplishments:

Objective 1: Characterize the signal transduction pathway that regulates skeletal muscle growth and metabolism including the influence of endogenous growth factors and various production practices.

Alabama Station:

1. Characterization of myogenic stem cell heterogeneity and fiber morphometrics in two divergently selected broiler chicken lines.
2. Completed the live animal, sample collection, and data collection portions of the project.
3. Presented an abstract at the 2017 Poultry Science Association annual meeting.
4. Impact of in ovo thermal manipulation on broiler chicken muscle development, growth, and satellite cell activity.
5. Completed the live animal growth performance and carcass yield data collection and sample collection portions of the project.
6. The cryohistology and immunofluorescence analysis portions of the project are ongoing.
7. Effects of dietary amino acid density on growth performance, satellite cell activity, collagen gene expression, and the incidence of wooden breast.
8. Completed the live animal growth performance and carcass yield data collection, sample collection, and data analysis portions of the project.
9. Presented an abstract at the 2017 Poultry Science Association annual meeting.
10. Submitted manuscript to a peer-reviewed journal, Poultry Science

Connecticut Station:

1. Effects of poor maternal nutrition during gestation on fetal muscle development
   1. Completed immunohistochemistry of fetal muscle samples at d45, d90, d135, and birth, determined muscle fiber CSA of primary and secondary fibers at d90, determined number of Pax7(+) progenitor cells at all four time points.
   2. Completed metabolome analysis of longissimus dorsi muscle from offspring of over-, restricted-, and control-fed ewes at day 45, 90, 135 of gestation and within 24 h of birth.
   3. Identified changes in lipid peroxidation as a result of maternal diet in serum and muscle in offspring within 24 h of birth.

 Hawaii Station:

1. Myostatin inhibitory region of pig myostatin propeptide

1. Background: Myostatin (MSTN) is a potent negative regulator of skeletal muscle growth, and its activity is suppressed by MSTN propeptide (MSTNpro), the N-terminal part of MSTN precursor cleaved during post-translational processing. We have previously shown that bioactive pig MSTNpro could be produced in an coli system. The current study examined which region of pig MSTNpro is critical for MSTN inhibition.
2. Method: Four truncated forms of pig MSTNpro containing N-terminal maltose binding protein (MBP) as a fusion partner were expressed in coli, and purified by affinity chromatography. The MSTN-inhibitory capacities of these proteins were examined by the pGL3-(CAGA)₁₂ luciferase reporter assay.
3. Results: A MBP-fused, truncated MSTNpro containing residues 42-175 (MBP-Pro42-175) exhibited the same MSTN-inhibitory potency as the full sequence MSTNpro. Truncated MSTNpro proteins containing either residues 42-115 (MBP-Pro42-115) or 42-98 (MBP-Pro42-98) also exhibited MSTN-inhibitory capacity with lower potencies than that of full sequence MSTNpro. Removal of MBP from MBP-Pro42-175 and MBP-Pro42-98 resulted in 20-fold decrease in MSTN-inhibitory capacity of Pro42-175 and abolition of MSTN-inhibitory capacity of Pro42-98, indicating that MBP as fusion partner enhanced the MSTN-inhibitory capacity of those truncated MSTNpro proteins. Interestingly, IC₅₀ value of MBP-Pro42-175 for MSTN inhibition was almost 4-fold lower than that for GDF-11 inhibition, while IC₅₀ value of full sequence MSTNpro for MSTN inhibition was not different from that for GDF-11 inhibition, indicating that MBP-Pro42-175 specifically inhibits MSTN with less cross-reactivity to GDF-11.

 Idaho Station

1. Completing an aquaculture feeding trial in sablefish (Anoplopoma fimbria) examining the influence of rearing temperature and dietary composition on growth traits and temporal expression of myogenic and metabolic genes in both white and red skeletal muscle. This comprises the final research trial for an MS student with projected completion of December 2017.

 Illinois Station:

1. Interaction of IGF2 and Myostatin in the Regulation of Muscle Growth and Development
   1. Pigs, heterozygous for a novel inactivating mutation of myostatin, were raised to market weight and characterized. These pigs had either the favorable IGF2 paternal A allele or the unfavorable paternal G allele (IGF G3072A). Live weights were similar between all genotypes (average 124kg) but weights of individual muscles were increased 10-30% in myostatin heterozygous pigs compared with wild type pigs. The effects of the favorable IGF2 allele and the myostatin mutation were completely additive.
   2. Mice were genetically edited using tail-effector-like endonuclease technology (TALEN) to mimic the naturally occurring IGF2 G3072A mutation. This resulted in three distinct lines of mice possessing the desire G>A point mutation only, the desired G>A point mutation with an additional A>G mutation at the target site, and C-insertion mutation. All mutations prevented the binding of a transcriptional repressor ZBED6. Preliminary data from characterization of these mice indicate that body weight and muscle weights are increased in all three mutated lines compared with wild type mice. However, in contrast to the similar mutation in pigs, this increase in body weight does not appear to be restricted to muscle. Organ weight and adipose tissue weight were also increased in these mutated mice.

 Indiana Station:

1. Pten is a phosphatase that antagonize growth factor (IGF1) signaling. We reported deletion of Pten in embryonic myoblasts leads to postnatal muscle hypertrophy but disrupts satellite cell homeostasis (Yue et al, 2016, Cell Reports).

 Iowa Station:

1. We completed tissue collection for our next experiment related to modification of the PGC-1α pathway via nutraceuticals. We have expanded our interventions to include quercetin, nicotinamide riboside, Lisinopril, and Prednisone and combinations thereof.  Preliminary analyses are underway and a histological and biochemical examination will begin soon. 

2. We confirmed dysfunctional autophagy in dystrophic skeletal muscle. Importantly, we are the first group to document release of autophagosomes, termed autophagosome escape, from dystrophic muscle.  Importantly, we also collected compelling evidence of this same phenomenon occurring in healthy muscle.  As they are found in the extracellular space they may participate in paracrine signaling and considering the mass of muscle and that they escape the muscle environment may participate in endocrine signaling. 
3. We have begun experiments to better understand why autophagy is dysfunctional in dystrophic muscle, which are now focused on regulation of transcription factor EB, the primary transcription factor driving lysosome biogenesis.

 Minnesota Station:

1. Protein synthesis was evaluated in equine satellite cell myotube cultures treated with a leucine titration ranging from 0- to 408-µM. Our results show a 1.8-fold increase (P < 0.02) in protein synthesis at levels slightly greater than those found in the general circulation, 204- and 408-µM when compared to a no leucine control (0-µM). Puromycin incorporation, a nonradioactive surface sensing of translation (SUnSET) methodology, demonstrated a 180% increase (P = 0.0056) in puromycin incorporation in leucine compared to control cultures. 
2. When equine satellite cell myotube cultures were treated with leucine (LEU; 408-µM) or a no-leucine control (CON) in the presence or absence of rapamycin (LR and CR, respectively), an inhibitor of mTOR, rapamycin, suppressed phosphorylation of mTOR (P < 0.01) and rS6 (P < 0.01) with an increase in phosphorylation of rS6 in leucine-treated cultures observed when compared to control cultures (P < 0.05). Similarly, there was a 27% increase (P < 0.005) in the hyperphosphorylated γ-form of 4E-BP1 compared to total 4E-BP1 in LEU compared to CON cultures with leucine-induced phosphorylation of 4E-BP1 completely blocked by rapamycin with a smaller decrease observed in CR compared to CON cultures.   

Mississippi Station:

1. The effects of dietary lysine level on the blood plasma concentrations of protein, carbohydrate, and lipid metabolites were investigated in late-stage finishing pigs.
   1. The 3 dietary lysine levels were 0.43% (a deficient level; for Diet I), 0.71% (an adequate level; for Diet II), and 0.98% (an excess level, for Diet III).
   2. There were no differences (P > 0.10) between pigs fed Diets II and III in the plasma concentrations of urea nitrogen, albumin, and total cholesterol, the concentration of albumin was higher (P < 0.05) than that of pigs fed Diet I, and the concentrations of urea nitrogen and total cholesterol were lower (P < 0.05) than that of pigs fed Diet I.
   3. There were no differences in plasma insulin and GH concentrations (P > 0.05) between the three dietary treatments.
   4. Plasma IGF-1 concentration of the pigs fed either Diet I or Diet II was lower (P < 0.05) than that of the pigs fed Diet II.
   5. There were no differences (P > 0.10) among the three dietary treatments in the plasma concentrations of total protein, triglycerides, and glucose.

 New Jersey Station:

1. Delineated the effects of acute strenuous exercise, as well as a 12-week exercise-training program, on plasma amino acids and the skeletal muscle metabolome in mature, Standardbred horses. These measurements revealed fitness to differentially alter lipid metabolism, branched-chain amino acid metabolism and nucleotide metabolism following acute exercise. Expression (mRNA levels) of the transcription factor ATF4 and other genes known to regulate metabolism are currently being measured in the same skeletal muscle samples. This work is under manuscript preparation and thesis preparation.
2. Examined long term control of muscle protein synthesis in mice fed a sulfur amino acid restricted diet. Results show that dietary sulfur amino acid restriction reduces muscle protein synthesis. Genetic deletion of the amino acid sensor GCN2 in mice did not rescue this decline in skeletal muscle protein synthesis.

 Utah Station:

1. Determination of mechanism through which decreased plane of nutrition in second trimester alters end-product quality of offspring in beef cattle
   1. Samples were collected from offspring of mother cows that either maintained BCS during the second trimester (MAIN) or from cows that dropped one BCS during the second trimester of pregnancy. Samples were collected from the longissimus dorsi at weaning, prior to beginning the feedlot phase and immediately following harvest.
   2. Completed miRNA analysis of samples from weaning, the beginning of the feedlot phase and immediately following harvest. Ten different miRNA were analyzed using qRT-PCR methods.
2. Gained insight into how different organic pastures impact dairy heifer development.
   1. A total of 6 animals per treatment were used to study 8 different pastures.
   2. Animal growth, serum IGF-1 concentration, blood urea nitrogen concentration, and parasite load were measured.

 Washington Station:

1. During the past years, we have been focused on the impacts of maternal nutrition on the early development of skeletal muscle and brown adipose tissue.
2. We also explored the role of AMPK in regulating brown adipose tissue development.
   1. We found that AMPK regulates brown adipogenesis through providing a key metabolite, alpha-ketoglutarate, which is required for DNA demethylation in the Prdm16 promoter and brown adipogenesis.
3. In addition, we are also defining the role of vitamin A and its metabolite, retinoic acid, in mediating adipose tissue development in beef cattle.

Wyoming Station:

1. RBM20 regulates splicing essential for myofiber structure and skeletal muscle physiology.
   1. Completed animal experiments. 3 months and 6 months old WT and RBM20 KO male Sprague-Dawley rats are used to study the differences in myofiber structure and skeletal muscle physiology. Skeletal muscles (longissimus dorsi, soleus, extensor digitorum longus, tibialis anterior and gastrocnemius) were collected. The tissues were snap-frozen in liquid nitrogen and stored at -80 celsius degree or embedded in OCT medium for cryosectioning.
   2. Completed the study of RBM20 expression in different types of skeletal mucle (longissimus dorsi, soleus, extensor digitorum longus, tibialis anterior and gastrocnemius).
   3. Completed myofiber structural and physiological analysis in skeletal muscle. We studied the change in muscle mass and myofiber cross sectional area between WT and RBM20 KO rat skeletal muscles (soleus, extensor digitorum longus, tibialis anterior). In addition, we studied fibrosis development and sarcolemma integrity in these skeletal muscles.Completed myofilament protein analysis in skeletal muscles. We investigated myosin heavy chain type distribution in WT and RBM20 KO rat skeletal muscles (soleus, extensor digitorum longus, tibialis anterior).
2. Effects of maternal obesity (MO) during gestation on fetal muscle function and development
   1. Completed the molecular mechanism study of the effect of MO on cardiac muscle contractility and calcium insensitivity in offspring of MO ewes. We found that MO reduced expression of myosin heavy chain in fetal myocardium. Moreover, the cardiac troponin T and troponin I expression level was upregulated in fetuses from obese mothers, whereas MO downregulated the expression of troponin C in fetal myocardium. Also, we found that MO increased the phosphorylation of protein kinase A (PKA) as well as the phosphorylation of Ca²⁺/calmodulin-dependent protein kinase II (CaMKII), which further promoted phosphorylation level of ryanodine receptor type 2 (Ryr2). Increased CaMKII and PKA phosphorylated Ryr2 plays a critical role in the development of MO-induced sarcoplasmic reticulum Ca²⁺ leak in fetal cardiomyocytes.
   2. Elevated cortisol level in offspring of obese ewes induces autophagic gene up-regulation in heart muscle. We found that the autophagic cargo protein SQSTM1, also known as ubiquitin-binding protein p62, is upregulated in fetal heart from obese ewes, and RNA binding motif 39 (RBM39), a transcriptional factor is activated by cortisol stimulation. our studies reveal autophagy pathways have been activated via RBM39 transcriptional regulation in fetal myocardium of obese ewes, as well as the autophagy cargo protein SQSTM1.

Objective 2: Characterize the cellular and molecular basis of myogenesis

 Alabama Station:

1. Characterization of myogenic stem cell heterogeneity and fiber morphometrics in two divergently selected broiler chicken lines.
2. Completed the live animal, sample collection, and data collection portions of the project.
   1. Presented an abstract at the 2017 Poultry Science Association annual meeting.
3. Impact of in ovo thermal manipulation on broiler chicken muscle development, growth, and satellite cell activity.
   1. Completed the live animal growth performance and carcass yield data collection and sample collection portions of the project.
   2. The cryohistology and immunofluorescence analysis portions of the project are ongoing.
4. Effects of dietary amino acid density on growth performance, satellite cell activity, collagen gene expression, and the incidence of wooden breast.
   1. Completed the live animal growth performance and carcass yield data collection, sample collection, and data analysis portions of the project.
   2. Presented an abstract at the 2017 Poultry Science Association annual meeting.
   3. Submitted manuscript to a peer-reviewed journal, Poultry Science

 Arkansas Station:

1. Myogenesis and adipogenesis in muscle is affected by n-3 fatty acids supplement for cell culture
   1. Preliminary studies were conducted using a murine in vitro myoblasts model (C₂C₁₂) to detect the change of mitochondria biosynthesis, function, and pathways of adipogenesis caused by n-3 fatty acid (EPA and DHA) treatment.
   2. EPA and DHA were added into culture media to mimic the maternal over-nutrition during gestation.
   3. In the myogenesis, data show fatty acids treatment limits the formation of myotubes, as well as the marker genes expression of myogenesis. Genes expression related to adipogenesis are upregulated by fatty acids treatment. Mitochondrial biosynthesis is inhibited by fatty acids, and it is confirmed by analyzing mitochondrial respiration, which shows fatty acids treatment decreases the oxygen consumption rate. Peroxisomes biosynthesis genes were upregulated by fatty acids.
   4. In white adipogenesis, key genes related to mitochondrial synthesis and metabolism (especially those related to complexes enzymatic activities) were significantly down-regulated by n-3 fatty acids treatment. While during brown adipogenesis, n-3 fatty acids treatment improves ATP synthase, mitochondrial biogenesis, and brown adipogenesis up-stream regulators gene expression.
2. Gene edition technology in transgenic beef production
   1. Galactose-α-1,3-galactose (Alpha-Gal) is a mammalian carbohydrate compound that presents in vibrate animals except humans or Old World monkeys. Alpha-Gal is synthesized by a glycosylation enzyme α-1,3-Galactosyltransferase (GGTA1).
   2. Genetically knocking out GGTA1 in pig protects xenotransplantation from hyperacute rejection
   3. We have designed target gRNAs for the bovine GGTA1 gene. Next step is using CRISPR-Cas9 to edit GGTA1 gene in bovine primary cell culture.
   4. We used the gRNA design tool and selected 5'-GGCCTGACGGTTTTCGCCGT-3' as the target gRNA sequence from the coding DNA sequence of Bos taurus alpha-galactosyltransferase 1 (glycoprotein). The gRNA was constructed in the pSpCas9 BB-2A-GFP (PX458) vector provided by GenScript USA Inc. Vectors were amplified and transfected into BAOSMC by GenePORTER2 transfection reagent when the cells were 80% confluency. Green fluorescent can be viewed after 24 hours transfection. The transfection efficiency can reach about 70% to 80%.
   5. Cells were collected in PBS at pH7.4 after 24 hours transfection. Total protein was extracted then the enzyme-linked immunosorbent assay was used to examine the GGTA1 production. By normalized with the total protein concentration, the GGTA1 protein level in the transfected cells was 17.9 ± 7.25% lower (P< 0.05) than in the control cells, showing a significant inhibition of GGTA1 gene expression in the cells by CRISPR-Cas9 gene edition method.
   6. Our preliminary data shows that the gRNA sequence that we chose was suitable for the GGTA1 gene knockout in bovine aortic smooth muscle cells. Moreover, the CRISPR-Cas9 system was proved can be applied in the genome editing of bovine cells.

 Illinois Station:

1. Influence of maternal infection or oxidative stress on muscle development and epigenetic programming of pigs
   1. During mid-gestation, pregnant sows were inoculated with porcine respiratory and reproductive virus (PRRS) and piglets from these sows were compared with those born of non-infected dams. Muscle cell number in the semitendinosus muscle was reduced 30% in piglets from infected dams. Methylation of analysis of longissimus dorsi muscle of newborn piglets from infected sows revealed differential methylation patterns resulting from maternal infection. Hypermethylation was present in several disease response pathways, in the thyroid hormone and oxidative phosphorylation pathways and in genes involved in fast muscle fiber phenotypes. Coupled with an increase in oxidative myosin heavy chain fiber type gene expression in these animals, these data suggest that maternal infection may limit muscle development and shift muscle fiber types to a more slow, oxidative phenotype.
   2. Beginning at 30 d of pregnancy and continuing until parturition, oxidative stress was induced in sows by feeding soybean oil that had been cooked at 90C for 72 hours. Piglet viability, growth, and muscle development were analyzed and compared with piglets from sows fed fresh oil. Piglets born from sows fed heated oil had reduced heart, lung, and liver weights, and reduced small intestine length compared with piglets born from mothers fed fresh oil. Muscle weight, however, was not affected by oxidative stress. Immunophenotyping revealed sows fed oxidized oil had an approximate 11% reduction in total T-cell population, with an increase in CD4+CD8+ double positive T-cells, compared with sows fed fresh oil.

 Indiana Station:

1. We demonstrated that the transcriptional factor Ascl2 is transiently expressed in a subpopulation of embryonic myoblasts to promote the generation of satellite cells during development. Ascl2 achieves this role through inhibiting the transcriptional activity of myogenic regulatory factors (Wang et al, 2017a, Development).
2. We used CRISPR/CAS9-mediated gene targeting to demonstrate that loss of MyoD promotes fate transdifferentiation of myoblasts into brown adipocytes (Wang et al 2017b, ebiomedicine).
3. We discovered that deletion of PTEN in satellite cells leads to their rapid depletion due to premature differentiation. Furthermore, we found that PTEN interacts with Notch signaling to maintain satellite cell quiescence (Yue et al, 2017 Nature Communications).
4. Using conditional knockout mouse models, we demonstrate that the hypoxia inducible factors HIF1α and HIF2α are dispensable for embryonic muscle development, but essential for postnatal muscle regeneration (Yang et al, 2017, JBC).

 Michigan Station:

1. We studied the effects of thermal challenge on growth of turkey satellite cells

 Mississippi Station:

1. The effects of dietary lysine level on the skeletal muscle gene expression were investigated in late-stage finishing pigs using transcriptomic microarray analysis.
   1. The 3 dietary lysine levels were 0.43% (a deficient level; for Diet I), 0.71% (an adequate level; for Diet II), and 0.98% (an excess level, for Diet III).
   2. The results revealed that 674 transcripts were differentially expressed (at P £05 level). At the P £ 0.01 level, 60 out of 131 transcripts were annotated in the NetAffx database.
   3. Ingenuity pathway analysis showed that dietary lysine deficiency may lead to: (1) increased muscle protein degradation via the ubiquitination pathway as indicated by the up-regulated DNAJA1, HSP90AB1 and UBE2B mRNA; (2) reduced muscle protein synthesis via the up-regulated RND3 and ZIC1 mRNA; (3) increased serine and glycine synthesis via the up-regulated PHGDH and PSPH mRNA; and (4) increased lipid accumulation via the up-regulated ME1, SCD, and CIDEC mRNA.
   4. Dietary lysine excess may lead to: (1) decreased muscle protein degradation via the down-regulated DNAJA1, HSP90AA1, HSPH1, and UBE2D3 mRNA; and (2) reduced lipid biosynthesis via the down-regulated CFD and ME1 mRNA.
2. Completed sample collection on study investigating the effects of melatonin supplementation on fetal porcine muscle development. Pregnant sows were supplemented with (n = 6) or without (n = 6) melatonin 920mg/hd/d) from 30-90 days of gestation at which point they were harvested to collect the developing fetuses for morphological and histological analyses. These offspring were necropsied and the semitendinosus and longissimus dorsi muscles were collected for subsequent analysis.
   1. Immunohistochemistry of fetal muscle samples to determine muscle fiber CSA and fiber type distribution is underway.
   2. Quantitative PCR for expression of mRNA and miRNA involved in muscle growth and development are underway.

 North Carolina Station:

1. Increased myostatin expression, resulting in muscle loss, has been associated with hyperammonemia in mammalian models of cirrhosis.
   1. However, there is evidence that hyperammonemia in avian embryos results in a reduction of myostatin expression, suggesting a proliferative myogenic environment.
   2. Increased myostatin expression, resulting in muscle loss, has been associated with hyperammonemia in mammalian models of cirrhosis. However, there is evidence that hyperammonemia in avian embryos results in a reduction of myostatin expression, suggesting a proliferative myogenic environment.
   3. The present in vitro study examines species differences in myotube and liver cell response to ammonia using avian and murine-derived cells.
   4. Relative expression of myostatin mRNA, determined by quantitative real-time PCR, was significantly increased in AA (10 mM) treated C2C12 myotubes compared to both ages of chick embryonic myotube cultures after 48 h (P < 0.02). Western blot analysis of myostatin protein confirmed an increase in myostatin expression in AA-treated C2C12 myotubes compared to the sodium acetate (SA) controls, while myostatin expression was decreased in the chick embryonic myotube cultures when treated with AA.
   5. Myotube diameter was significantly decreased in AA-treated C2C12 myotubes compared to controls, while avian myotube diameter increased with AA treatment (P < 0.001). There were no significant differences between avian and murine liver cell viability, assessed using 2', 7'- bis-(2-carboxyethyl)-5-(and-6-)-carboxyfluorescein, acetoxymethyl ester, when treated with AA. However, after 24 h, AA-treated avian myotubes showed a significant increase in cell viability compared to the C2C12 myotubes (P < 0.05).

 Ohio Station:

1. Effect of Thermal Stress on In Vivo Breast Muscle Growth and Development in Broilers: Association of Wooden Breast with collagen crosslinking
   1. Poultry selected for growth have an inefficient thermoregulatory system and are more sensitive to temperature extremes.
   2. Satellite cells are precursors to skeletal muscle and mediate all posthatch muscle growth. Their physiological functions are affected by temperature.
   3. The objective of the current study was to elucidate the effects of continuous heat exposure the first 2 wk of age on breast muscle development in broilers
   4. Results showed a high level of sensitivity in the satellite cells during the early posthatch period to chronic heat, leading to impaired myogenicity and increased fat.
   5. Results on Wooden Breast studies have shown the possibility of multiple fibrotic myopathies based on collagen crosslinking. The repair and regeneration of muscle fibers mediated by satellite cells appears to be suppressed

 Wyoming Station:

1. RBM20 mediates skeletal muscle regeneration after injury.
   1. Completed animal experiments. 9 weeks old WT and RBM20 KO male Sprague-Dawley rats are used to study skeletal muscle regeneration. Tibialis anterior muscle was injured with the injection of 0.5ml of 1.2% of barium chloride and injured muscles are harvested at 18 h, 3 days, 5days, 7 days, 14 days, 1 month and 2 months post-injury. The other hindlimb tibialis anterior muscle was injected with PBS as control. After harvesting the tissues are liquid nitrogen snap-frozen and stored at -80 celsius degree or embedded in OCT medium for cryosectioning.
   2. Completed RBM20 expression during skeletal muscle regeneration.
   3. Completed myofiber cross sectional area analysis during skeletal muscle regeneration.
   4. Completed analysis of fibrosis level after recovery.
   5. Completed analysis of expression of myogenic transcription factors during skeletal muscle regeneration.

Objective 3: Characterize mechanism of protein assembly and degradation in skeletal muscle

Utah Station:

1. Gained insight into the molecular mechanism responsible for development of beef tenderness during aging.
   1. Samples were collected from the longissimus dorsi of steaks immediately post-mortem and also hafter 14 days of aging in 100 samples.Samples were then analyzed for tenderness with WBSF methods.
   2. Protein expression of HSPβ1, HSP70, and PARK7 is currently being analyzed for all 100 samples at two different sample collection time points.
2. Increased our understanding of how beta-agonist and implant administrations alters tenderness.
   1. Steaks were collected from the LL of feedlot heifers that received one of three different treatments (n = 11 per treatment) during the feedlot phase: no anabolic implant or beta-agonist (CON), anabolic implant but no beta-agonist (IMP), or an anabolic implant and a beta-agonist (COMBO)
   2. Steaks were aged for 3, 7, 14, 21 or 35 d and samples were analyzed for protein expression of HSPβ1 and HSP70.

Publications (peer reviewed journals):

1. Hoffman, M. L., S. A. Reed, S. M. Pillai, A. K. Jones, K. K. McFadden, S. A. Zinn, and K. E. Govoni. The effects of poor maternal nutrition during gestation on offspring postnatal growth and metabolism. J Anim Sci. 94:3093-3099. doi:10.2527/jas.2016-1229
2. Pillai, S. M., A. K. Jones, M. L. Hoffman, K. K. McFadden, S. A. Reed, S. A. Zinn, and K. E. Govoni. Fetal and organ development at gestational days 45, 90, 135 and at birth of lambs exposed to under- or over-nutrition during gestation. Translational Anim. Sci. doi: 10.2527/tas2016.0002
3. Sang Beum Lee, Sung Kwon Park and Yong Soo Kim. 2017. Maltose binding protein-fusion enhances the bioactivity of truncated forms of pig myostatin propeptide produced in E. coli. Plos One 12(4): e0174956.
4. Jin-Dan Kang, Seokjoong Kim, Hai-Ying Zhu, LongJin, QingGuo, Xiao-Chen Li, Yu-ChenZhang, Xiao-XuXing, Mei-Fu Xuan, Guang-Lei Zhang, Qi-Rong Luo, Yong Soo Kim, Cheng-Du Cui1, Wen-XueLi1, Zheng-YunCui1, Jin-Soo Kim, and Xi-Jun Yin. 2017. Generation of cloned adult muscular pigs with myostatin gene mutation by genetic engineering. RSC Advances 7:12541-12549.
5. D. Berrocoso, R. Kilda, A.K. Singh, Y.S. Kim and R. Jha. 2017. Effect of in ovo injection of raffinose on growth performance and gut health parameters of broiler chicken. Poultry Science 96:1573-1580
6. J. Colle, J.A. Nasados, J.M. Rogers, D.M. Kerby, M.M. Colle, J.B. Van Buren, R.P. Richard, G.K. Murdoch, C.J. Williams, M.E. Doumit , Strategies to improve beef tenderness by activating calpain-2 earlier postmortem. (2017) Meat Science doi: 10.1016/j.meatsci.2017.08.008
7. J. Thornton, K.C. Chapalamadugu, E. M. Eldredge and G.K. Murdoch Analysis of Longissimus thoracis protein expression associated with variation in carcass quality grade and marbling of beef cattle raised in the Pacific northwestern United States. (2017) Journal of Agricultural and Food Chemistry January 24 DOI 10.1021/acs.jafc.6b02795
8. M. Murdoch, G. K. Murdoch, S. Greenwood, S. McKay Nutritional Influence on Epigenetic Marks and Effect on Livestock Production. (2016) Frontiers in Genetics: nutrigenomics., doi: 10.3389/fgene.2016.00182
9. Chen Y, Wang J, Yang S, Utturkar S, Crodian J, Cummings S, Thimmapuram J, San Miguel P, Kuang S, Gribskov M, Plaut K, Casey T. 2017. The Effect of High Fat Diet on Secreted Milk Transcriptome in Mid-lactation Mice. Physiol Genomics, in press
10. Xiong Y, Page JC, Narayanan N, Wang C, Jia Z, Yue F, Shi X, Jin W, Hu K, Deng M, Shi R, Shan T, Yang G, Kuang S*. 2017. Peripheral neuropathy and hindlimb paralysis in a mouse model of adipocyte-specific knockout of eBioMedicine. DOI: http://dx.doi.org/10.1016/j.ebiom.2017.09.017
11. Jiang C, Cano Vega MA, Yue F, Kuang L, Narayanan N, Uzunalli G, Merkel MP, Kuang S*, Deng M*. 2017. Dibenzazepine-loaded Nanoparticles Induce Local Browning of White Adipose Tissue to Counteract Obesity. Mol Ther. 25(7):1718-29. doi: 10.1016/j.ymthe.2017.05.020.
12. Wang C, Yue F, Kuang S*. 2017. Muscle Histology Characterization Using H&E Staining and Muscle Fiber Type Classification Using Immunofluorescence Staining. Bio-Protocol. 7(10): e2279. DOI: 10.21769/BioProtoc.2279.
13. Castro B, Kuang S*. 2017. Evaluation of Muscle Performance in Mice by using the Treadmill Exhaustion Test and the Whole-Limb Grip Strength Assay. Bio-Protocol. 7(8): e2237. DOI: 10.21769/BioProtoc.2237
14. Yang X, Yang S, Wang C, Kuang S*. 2017. The hypoxia inducible factors HIF1α and HIF2α are dispensable for embryonic muscle development but essential for postnatal muscle regeneration. J Biol Chem. 292(14):5981-91. doi:10.1074/jbc.M116.756312
15. Wang X, Yu C, Chen J, Jiang Q, Kuang S, Wang Y. 2017. Depot-specific differences in fat mass expansion in WT and ob/ob mice. Oncotarget. 8(28):46326-36. doi: 10.18632/oncotarget.17938.
16. Zhang B, Shen Q, Chen Y, Pan R, Kuang S, Liu G, Sun G, Sun X. 2017. Myricitrin alleviates oxidative stress-induced inflammation and apoptosis and protects mice against diabetic cardiomyopathy. Sci Rep. 7:44239. doi: 10.1038/srep44239.
17. Wang C, Liu W, Nie Y, Qaher M, Horton HE, Yue F, Asakura A, Kuang S*. 2017b. Loss of MyoD promotes fate transdifferentiation of myoblasts into brown adipocytes. eBiomedicine. 16:212-23.
18. Yue F, Bi P, Yang X, Wang C, Shan T, Nie Y, Ratliff TL, Gavin TP, Kuang S*. 2017. Pten is necessary for the quiescence and maintenance of adult muscle stem cells. Nat Commun. 8:14328.
19. Yin F, Yang S, Hu S, Kuang S*, Han Q*. 2017. Enhanced human osteoblast cell functions by “net-like” nanostructured cell-substrate interface in orthopedic applications. Mater Lett. 189:275-8.
20. Zhang C, Huang KC, Rajwa B, Li J, Yang S, Lin H, Liao CS, Eakina G, Kuang S, Patsekin V, Robinson JP, Cheng JX. 2017. Stimulated Raman Scattering Flow Cytometry for Label-Free Single-Particle Analysis. 4(1): 103-9. doi:10.1364/OPTICA.4.000103
21. Wang C, Wang M, Arrington J, Shan T, Yue F, Nie Y, Tao WA, Kuang S*. Ascl2 inhibits myogenesis by antagonizing the transcriptional activity of myogenic regulatory factors. Development. 144(2):235-247. doi: 10.1242/dev.138099.
22. Yue F, Bi P, Wang C, Li J, Liu X, Kuang S*. 2016. Conditional loss of Pten in myogenic progenitors leads to postnatal skeletal muscle hypertrophy but age-dependent exhaustion of satellite cells. Cell Rep. 17(9):2340-53. doi: 10.1016/j.celrep.2016.11.002.
23. Spaulding H, Kelly E, Quindry JC, Sheffield J, Hudson MB, and Selsby J. Autophagic dysfunction and autophagosome escape in the mdx mus musculus model of Duchenne muscular dystrophy.  Acta Physiologica.  In Press. 
24. Ballmann C, Denney CT, Beyers R, Quindry T, Romero T, Selsby JT, and Quindry JC. Long term dietary quercetin enrichment as a cardioprotective countermeasure in mdx mice.  Experimental Physiology. 102:635-649, 2017. 

*This paper was featured in an unsolicited ViewPoint from Experimental Physiology. 

25. Ballmann C, Denney T, Beyers R, Quindry T, Romero M, Amin R, Selsby JT, and Quindry JC. Lifelong quercetin enrichment and cardioprotection in Mdx/Utrn^+/-   American Journal of Physiology: Heart and Circulation.  312:128-140, 2017
26. Spaulding HR, Ballmann CG, Quindry JC, and Selsby JT. Long-term quercetin dietary enrichment partially protects dystrophic skeletal muscle.  PLoS One.  11: e0168293, 2016. 
27. Clark, D,L,; Strasburg, G,M,; Reed, K.M.; Velleman, S.G. 2017. Influence of temperature and growth selection on turkey pectoralis major muscle satellite cell adipogenic gene expression and lipid accumulation. Poultry Science 96:1015-1027.
28. Reed, K.M.; Mendoza, K.M.; Abrahante, J.E.; Barnes, N.E.; Velleman, S.G., Strasburg, G.M. 2017. Response of turkey muscle satellite cells to thermal challenge. I. Transcriptome effects in proliferating cells. BMC Genomics 18(1):352. doi: 10.1186/s12864-017-3740-4
29. Reed, K.M.; Mendoza, K.M.; Strasburg, G.M.; Velleman, S.G. 2017. Response of turkey muscle satellite cells to thermal challenge. II. transcriptome effects in differentiating cells. Submitted to Frontiers in Avian Physiology.
30. DeBoer, M.L., K. M. Martinson, M. S. Pampusch, A. M. Hansen, S. M. Wells, C. Ward, M. Hathaway. 2017. Cultured equine satellite cells as a model system to assess leucine stimulated protein synthesis in horse muscle. Anim.Sci. (in press)
31. Pettit AP, Jonsson WO, Bargoud AR, Mirek ET, Peelor FF 3rd, Wang Y, Gettys TW, Kimball SR, Miller BF, Hamilton KL, Wek RC, Anthony TG. (2017) Dietary Methionine Restriction Regulates Liver Protein Synthesis and Gene Expression Independently of Eukaryotic Initiation Factor 2 Phosphorylation in Mice. J Nutr. Jun;147(6):1031-1040. doi: 10.3945/jn.116.246710. Epub 2017 Apr 26. PMID: 28446632
32. Shoeibi, S. P. Mozdziak, A. Golkar-Narenji, 2017. Biogenesis of selenium nanoparticles using green chemistry. Topics in Current Chemistry (Submitted).
33. Farzaneh, M., F. Attari, S. E. Khoshnam, and P. E. Mozdziak, 2017. Chicken whole embryo culture system, the strong in-vitro model, British Poultry Science (Submitted).
34. Zand-vakili, M. A. Golkar-Narenji, H. Eimani, and P. E. Mozdziak, 2017. An in vitro study on cumulus oocyte complexes COCs and follicles of autotransplanted mouse ovaries with the effect of VEGF. Journal of the Turkish German Gynecological Association (In Press).
35. Farzaneh, M, S. E. Khoshnam, and P.E. Mozdziak, 2017. The evolution of chicken stem cell culture methods. British Poultry Science DOI: 10.1080/00071668.2017.1365354.
36. Farzaneh, M, S. E. Khoshnam, and P.E. Mozdziak. 2017. Concise review: avian multipotent stem cells as a novel tool for investigating cell based therapies. J. Dairy Veterinary Animal Research 5: DOI: 10.15406/jdvar.2017.05.00125.
37. Farzaneh, M, S. N. Hossani, P.E. Mozdziak, and H. Baharvand. 2017. Avian embryos and related cell lines: a convenient platform for recombinant proteins and vaccines production. Biotechnology Journal 12: DOI 10.1002/biot.201600598/
38. Stern, R., A. S. Dasarathy, and P.E. Mozdziak, 2017. Ammonia elicits a different myogenic response in avian and murine myotubes. In Vitro Cell Dev. Biol. Animal. 53: 99-110.
39. Bazgir, B., R. Fathei, M. Rezazadeh Valoujerd, P. Mozdziak, and A. Asgari, 2017. Satellite cell contribution to cxercise mediated muscle hypertrophy and repair: A review article. Cell Journal 18: 473-484.
40. Powell, D.J., Velleman, S.G., Cowieson, A.J., and Muir, W.I. 2016. Methionine concentration in the pre-starter diet – its effect on broiler breast muscle development. Animal Production Sci. dx.doi.org/10.1071/AN15479.
41. Clark, D.L. Coy, C.S., Strasburg, G.M., Reed, K.M., and Velleman, S.G. 2016.Temperature Effect on Proliferation and Differentiation of Satellite Cells from Turkeys with Different Growth Rates. Poult. Sci. 95:934-947. (selected by the Editor-in-Chief as a Choice Publication based on content).
42. Harding, R.L., and Velleman, S.G. 2016. MicroRNA regulation of myogenic satellite cell proliferation and differentiation. Mol. Cell. Biochem. 412:181-195.
43. Powell, D.J., Velleman, S.G., Cowieson, A.J., Singh, M., and Muir, W.I. 2016. Influence of hatch time and access to feed on intramuscular adipose tissue deposition in broilers. Poult. Sci. 95:1449-1456.
44. Powell, D.J., Velleman, S.G., Cowieson, A.J., Singh, M., and Muir, W.I. 2016. Influence of chick hatch time and access to feed on broiler muscle development. Poult. Sci. 95:1433-1448. (selected by the Editor-in-Chief as a Choice Publication based on content).
45. Harding, R.L., Halevy, O., Yahav, S., and Velleman, S.G. 2016. The effect of temperature on proliferation and differentiation of chicken skeletal muscle satellite cells isolated from different muscle types. Physiological Reports 4 (8), e12770.
46. Clark, D.L., and Velleman, S.G. 2017. Spatial influence on breast muscle morphological structure, myofiber size, and gene expression associated with the wooden breast myopathy in broilers. Poult. Sci. 95:2930-2945. dx.doi.org/10.3382/ps/pew243. (selected by the Editor-in-Chief as a Choice Publication based on content).
47. Clark, D.L., Strasburg, G.M., Reed, K.M., and Velleman, S.G. 2017. Influence of temperature and growth selection on turkey pectoralis major muscle satellite cell adipogenic gene expression and lipid accumulation. Poult. Sci. 96:1015-1027, dx.doi.org/10.3382/ps/pew374 selected by the Editor-in-Chief as a Choice Publication based on content).
48. Velleman, S.G. and Harding, R.L. 2017. Regulation of Turkey Myogenic Satellite Cell Migration by MicroRNAs miR-128 and miR-24. Poult. Sci. 96:1910-1917.doi: 10.3382/ps/pew434.
49. Reed, K.M., Mendoza, K.M., Abrahante, J.E., Barnes, N.E., Velleman, S.G., and Strasburg, G.M. 2017. Response of turkey muscle satellite cells to thermal challenge. I. Transcriptome effects in proliferating cells. BMC Genomics 18:352. DOI 10.1186/s12864-017-3740-4.
50. Piestun, Y., Patael, T., Yahav, S., Velleman, S.G., and Halevy, O. 2017. Early posthatch thermal stress affects breast muscle development and satellite cell growth and characteristics in broilers. Poult. Sci.96: 2877-2888..
51. Clark, D.L., Walter, K. G., and Velleman, S.G. 2017. Incubation temperature and time of hatch impact broiler muscle growth and morphology. Poult . Sci. doi.org/10.3382/ps/pex202.
52. Velleman, S.G., and Song, Y. 2017. Development and growth of the avian pectoralis major (breast) muscle: Function of syndecan-4 and glypican-1 in adult myoblast proliferation and differentiation.. Frontiers in Physiol. doi. 10.3389/phys.2017.00577. (invited review).
53. Velleman, S.G., Clark, D.L., and Tonniges, J.R. 2017. Fibrillar collagen organization associated with the broiler wooden breast fibroitic myopathy. Avian Dis. In press.
54. Wang, B., F. Zhang, H. Zhang, Z. Wang, Y. Ma, M. J. Zhu, and M. Du. (2017). Alcohol intake aggravates adipose browning and muscle atrophy in cancer associated cachexia. Oncotarget, In press.
55. Wang, B., X. Fu, X. Liang, J. M. Deavila, Z. Wang, L. Zhao, Q. Tian, J. Zhao, N. A. Gomez, S. C. Trombetta, M. J. Zhu, and M. Du. (2017). Retinoic acid induces white adipose tissue browning by increasing adipose vascularity and inducing beige adipogenesis of PDGFRa+ adipose progenitors. Cell Discovery, In press.
56. Fu, X., Q. Yang, B. Wang, J. Zhao, M. Zhu, S. M. Parish, and M. Du. (2017). Reduced satellite cell density and myogenesis in Wagyu compared to Angus cattle as a possible explanation of its high marbling. Animal, In press.
57. Wang, B., Z. Wang, J. M. de Avila, M. J. Zhu, F. Zhang, N. A. Gomez, L. Zhao, Q. Tian, J. Zhao, J. Maricelli, H. Zhang, B. D. Rodgers, and M. Du. (2017). Moderate alcohol intake induces thermogenic brown/beige adipocyte formation via elevating retinoic acid signaling. FASEB Journal, 31: 4612-4622.
58. Wang, B., X. Fu, M.J. Zhu, and M. Du. (2017). Retinoic acid inhibits white adipogenesis by disrupting GADD45A mediated Zfp423 DNA demethylation. Journal of Molecular Cell Biology, 9: 338-349.
59. Zhao, J., Q. Yang, L. Zhang, X. Liang, X. Sun, B. Wang, Y. Chen, M. J. Zhu, and M. Du. (2017). AMPKa1 deficiency suppresses brown adipogenesis in favor of fibrogenesis during brown adipose tissue development. Biochemical and Biophysical Research Communications, 491: 508-514.
60. Du, M., S.P. Ford, and M. J. Zhu. (2017). Optimizing livestock production efficiency through maternal nutritional management and fetal developmental programming. Animal Frontiers, 7: 5-11.
61. Song, J., Q. Wang, M. Du, X. Ji, and X. Mao. (2017). Identification of dipeptidyl peptidase-IV inhibitory peptides from mare whey protein hydrolysates. Journal of Dairy Science, 100: 6885-6894.
62. Wang, S., X. Liang, Q. Yang, X. Fu, M. Zhu, B.D. Rodgers, Q. Jiang, M. V. Dodson, and M. Du. (2017). Resveratrol enhances brown adipocyte formation and function by activating AMP-activated protein kinase (AMPK) 1 in mice fed high-fat diet. Molecular Nutrition and Food Research, 61: 1600746.
63. Song, J.J., Q. Wang, M. Du, T. G. Li, B. Chen, X. Y. Mao. (2017). Casein glycomacropeptide-derived peptide IPPKKNQDKTE ameliorate high glucose-induced insulin resistance in HepG2 cells via activation of AMPK signaling. Molecular Nutrition and Food Research, 61: 1600301.
64. Wang B., X. Fu, X. Liang, Z. Wang, Q. Yang, T. Zou, W. Nie, J. Zhao, P. Gao, M. J. Zhu, J. M. De Avila, J. Maricelli, B. D. Rodgers, and M. Du. (2017). Maternal retinoids increase PDGFRa progenitor population and beige adipogenesis in progeny by stimulating vascular development. EBioMedicine, 18:288-299.
65. Guan L., X. Hu, L. Liu, Y. Xing, Z. Zhou, X.W. Liang, Q. Yang, S. Jin, J. Bao, H. Gao, M. Du, J. Li, and L. Zhang. (2017). Bta-miR-23a involves in adipogenesis of progenitor cells derived from fetal bovine skeletal muscle. Scientific Report, 7:43716.
66. Griner, J. D., C. J. Rogers, M. J. Zhu, and M. Du. (2017). Lysyl oxidase propeptide promotes adipogenesis through inhibition of FGF-2 signaling. Adipocyte, 6: 12-19.
67. Zou, T., Q. Yang, B. Wang, M. Zhu, P. W. Nathanielsz, and M. Du. (2017). Resveratrol supplementation to high fat diet-fed pregnant mice promotes brown and beige adipocyte development and prevents obesity in male offspring. Journal of Physiology, 595: 1547-1562.
68. Li, T., B. Chen, M. Du, J. Song, X. Cheng, X. Wang, and X. Mao. (2017). Casein glycomacropeptide hydrolysates exert cytoprotective
69. Zhu C, Guo W. 2017. Detection and quantification of the giant protein titin by SDS-agarose gel electrophoresis. MethodsX. 4:320-327.
70. Guo W, Sun M. 2017. RBM20, a potential target for treatment of cardiomyopathy via titin isoform switching. Biophysical reviews. PMID: 28577155
71. Zhu C, Yin Z, Tan B, Guo W. 2017. Insulin regulates titin pre-mRNA splicing through the PI3K-Akt-mTOR kinase axis in a RBM20-dependent manner. Biochimica et biophysica acta. 1863(9): 2363-2371. PMID: 28676430
72. J. Thornton, K.C. Chapalamadugu, E.M. Eldredge, G.K. Murdoch. 2017. Analysis of longissimus thoracis protein expression associated with variation in carcass quality grade and marbling of beef cattle raised in the pacific northwestern United States. Journal of Agricultural and Food Chemistry. 65(7): 1434-1422. doi: 10.1021/acs.jafc.6b02795
73. Kamanga-Sollo, K.J. Thornton, M. E. White and W. R. Dayton. 2017. Role of G protein-coupled receptor-1, matrix metalloproteinases 2 and 9, and heparin binding epidermal growth factor-like growth factor in Estradiol-17β -induced alterations in protein synthesis and protein degradation rates in fused bovine satellite cell cultures. Domestic Animal Endocrinology. 58: 90-96 doi: 10.1016/j.domaniend.2016.09.002
74. Regmi, N., T. Wang, M. A. Crenshaw, B. J. Rude, and F. Liao. 2017. Effects of dietary lysine level on the concentrations of selected nutrient metabolites in blood plasma of late-stage finishing pigs. J. Anim. Physiol. Anim. Nutr. DOI: 10.1111/jpn.12714.
75. Wang, T., M. A. Crenshaw, M. S. Hasan, G. Wu, and F. Liao. 2017. Effects of dietary lysine levels on the plasma concentrations of growth-related hormones in late-stage finishing pigs. In T. Asao and M. Asaduzzaman. eds. Amino Acid - New Insights and Roles in Plant and Animal (ISBN: 978-953-51-3242-4). Chapter 13, pp. 259-271. InTech, Rijeka, Croatia. DOI: 10.5772/intechopen.68545.
76. Wang, T., J. M. Feugang, M. A. Crenshaw, N. Regmi, J. R. Blanton, Jr., and F. Liao. 2017. A systems biology approach using transcriptomic data reveals genes and pathways in porcine skeletal muscle affected by dietary lysine. Int. J. Mol. Sci. 18: 885 (1-20). DOI: 10.3390/ijms18040885.
77. Durfey, C. L., D. D. Burnet, F. Liao, C. S. Steadmana, M. A. Crenshaw, H. J. Clemente, S. T. Willard, P. L. Ryan, and J, M. Feugang. 2017. Nanotechnology-based selection of boar spermatozoa: growth development and health assessments of produced offspring. Livestock Sciences, 205: 137-142.

Book Chapters:

None

Abstracts, Posters, and Professional Presentations:

1. Morey, A., A. E. Smith, M. L. Johnson, L. J. Bauermeister, D. Starkey, R. Beyers, R. S. Moon, K. M. Cox, and W. D. Berry. 2017. Advanced Technologies for Detection and Analysis of Broiler Meat Quality with Novel Myopathies such as Woody Breast. World’s Poultry Science Association XXIII European Symposium on the Quality of Poultry Meat. EGGMEAT Edinburgh, Scotland.
2. Tejeda, O. J., J. A. Arana, A. J. Calderon, D. Starkey. 2017. Evaluation of broiler chicken myogenic stem cell population heterogeneity and skeletal muscle fiber morphometrics. Poul. Sci. Vol. 96 (E-Suppl 1).
3. Meloche, K. J., W. A. Dozier, III, D. Starkey. 2017. Skeletal muscle fiber morphometrics and in vivo myogenic stem cell mitotic activity in broiler chickens affected by Wooden Breast. Poul. Sci. Vol. 96 (E-Suppl 1).
4. Smith, A., S. I. Patton, R. Beyers, L. J. Bauermeister, D. Starkey, and A. Morey. 2017. Exploring magnetic resonance imaging, an advanced technology, to study modern meat quality defects such as wooden breast in broilers. Poul. Sci. Vol. 96 (E-Suppl 1).
5. Lock, A.L., K. M. Meloche, D. Starkey. 2017. Evaluation of objective digital methods for determination of Pectoralis major muscle cross-sectional area for use in estimation of skeletal muscle fiber number. International Poultry Scientific Forum. Atlanta, GA. Proceedings of the Southern Poultry Science Society. Poul. Sci. Vol. 96 (E-Suppl 1).
6. Pillai, S.M., M.L. Hoffman, A.K. Jones, K.K. McFadden, J.R. Stevens, S.A. Zinn, S.A. Reed, K.E. Govoni. Poor maternal nutrition during gestation alters muscle gene expression in fetal offspring. J Anim Sci. 2017. 95(E-Suppl 4):150
7. Martin, D.E., A. K. Jones, S. M. Pillai, M. L. Hoffman, K. K. McFadden, K. E. Govoni, S. A. Zinn, S. A. Reed. Effects of poor maternal nutrition and gender on satellite cell metabolism in lambs. J Anim Sci. 2017. 95(E-Suppl 4):37
8. Jones, A.K., S. M. Pillai, M. L. Hoffman, K. K. McFadden, K. E. Govoni, S. A. Zinn, S. A. Reed. Maternal restricted- and over-feeding during gestation alters offspring gene expression of inflammatory markers in the liver at d 135 of gestation and at birth. J Anim Sci. 2017. 95(E-Suppl 4):48
9. Wynn, M., A. K. Jones, M. L. Hoffman, S. M. Pillai, K. K. McFadden, S. A. Reed, S. A. Zinn, K. E. Govoni. The effects of poor maternal nutrition during gestation on the number of Pax7 positive myogenic progenitor cells. J Anim Sci. 2017. 95(E-Suppl 4):41
10. Breann N. Sandberg, Carl W. Hunt, Matthew E. Doumit, Ron Richard and Gordon K. Murdoch Effects of Rumen Protected-Histidine Supplementation Dose on Finishing Beef Cattle. accepted in the Meat Science and Muscle Biology section at the 2017 ASAS-CSAS Annual Meeting, Baltimore MD
11. Breann N. Sandberg, Carl W. Hunt, Matthew E. Doumit, Ron Richard and Gordon K. Murdoch Pacific Northwest Animal Nutrition Conference graduate research competition, January 2017, Richland, WA.
12. K. Hogan, J.E. Beever, and A.C. Dilger (2017) The Effects of Virally-Induced Maternal Inflammation on the Methylation Patterns of Skeletal Muscle in Offspring. FASEB Journal 31:979.2
13. Pautz C, Wilson BE, Jackson K, Selsby JT, Barerro CA, Merali S, Kelly EM, and Hudson MB. Exercise or reduced-calorie diet attenuates overnutrition-induced Glut4 carbonylations in adipose tissues.  ACSM, Denver, June, 2017.
14. Hudson MB, Pautz CM, Barrero CA, Kelly EM, Selsby JT, and Wilson BE. Size profile and selective protein packaging of exosomes released from atrophying muscle cells.  ACSM, Denver, June, 2017. 
15. Spaulding H, Kelly EM, Sheffield JB, Quindry JC, Hudson MB, and Selsby JT. Impaired autophagic flux in dystrophic muscle augments extracellular autophagosome release.  Advances in Skeletal Muscle Biology in Health and Disease.  Gainesville, FL, March 8-10, 2017. 
16. Spaulding H, Ross JW, Nonneman JD, and Selsby JT. Autophagy is independent of disease progression in the dystrophic myocardium in mouse and porcine dystrophinopathy models.  FASEB, Chicago, April, 2017.
17. Quindry J, Quindry T, Ballmann C, and Selsby JT. Indices of autophagy are unaltered by quercetin consumption in hearts of Mdx/Utrn^+/-  Experimental Biology, Chicago, April 22-26, 2017
18. Strasburg, G.M. 2016. Influence of thermal challenge on turkey meat quality. Oral presentation at the NC1184 USDA Annual Multistate Project Meeting. October 21, 2016, Manhattan, Kansas
19. Barnes, N.E.; Strasburg, G.M.; Velleman, S.G.; Reed, K.M. 2017. Transcriptional Response to Thermal Stress in Turkey Muscle. Poster 1134. Plant and Animal Genome Meeting
20. Reed, K.M.; Velleman, S.G.; Strasburg, G.M. 2017 Temperature effects on differential gene expression in turkey satellite cells during proliferation and differentiation. Presentation W765, Plant and Animal Genome Meeting, January 14 – 18, 2017. San Diego, CA
21. Strasburg, G.M.; Clark, D.L.; George, G.; Reed, K.M.; Velleman, S.G. 2017. Effect of embryonic and post-hatch thermal challenge on turkey muscle development. Presentation W766, Plant and Animal Genome Meeting, January 14 – 18, 2017. San Diego, CA
22. Presentation: Glaxo Smith Klein, “BCAA Catabolism and the integrated stress response”, December 9, 2016
23. Presentation: Acute exercise and training distinctively alter branched-chain amino acid metabolic signatures in the skeletal muscle of Standardbred horses. NIDDK Meeting: Emerging Roles of BCAAs in Human Health and Disease, May 25-26, 2017.
24. Presentation: School of Health Professions Nutritional Sciences Open Forum, “Dietary Methionine Restriction and the Integrated Stress Response”, Rutgers-Newark, June 15, 2017
25. Qiurong Wang, John F. Odhiambo, Chris Pankey, Adel Ghnenis,Peter W. Nathanielsz, Stephen P. Ford, Wei Guo. Molecular Basis of Maternal Obesity Induced Fetal Cardiac Contractile Dysfunction. The AHA scientific meeting, Anaheim, CA. November 11^th -15^th
26. Qiurong Wang¹, John F. Odhiambo¹, Peter W. Nathanielsz¹, Stephen P. Ford¹, Jun Ren², Wei Guo¹. RBM39 is Vital for Maternal Obesity-Induced Fetal Sheep Cardiac Contractile dysfunction by Regulating Myocardial Autophagy. The AHA scientific meeting, Anaheim, CA. November 11^th -15^th
27. Qiurong Wang^1,2, Chaoqun Zhu¹, John F. Odhiambo^1,2, Guadalupe L Rodríguez-González³, Peter W. Nathanielsz^1,2, Stephen P. Ford^1,2, Jun Ren⁴ and Wei Guo^1,2. Maternal Obesity Compromises Mitochondrial Bioenergetic Profile of Term Fetal Sheep Heart. 64^th SRI annual Scientific meeting, Orlando, FL. March 15^th -18^th (Oral Presentation)
28. G. Christensen, N.E. Ineck, K.J. Phelps , S.M. Ebarb , J.S. Drouillard , J.M. Gonzalez, K.J. Thornton. Small heat shock protein abundance differs during aging in steaks from the longissimus lumborum of cattle that received different growth promotants. Reciprocal Meats Conference, June 2017, College Station, TX.
29. E. Ineck, R.G. Christensen, S.M. Quarnberg, K.A. Rood, C.E. Carpenter, J.F. Legako, K.J. Thornton. Impacts of bovine maternal nutrition on miRNA expression in skeletal muscle of the progeny during growth. Reciprocal Meats Conference, June 2017, College Station, TX.
30. Thompson, R. C., K. J. McCarty, A. T. Sukumaran, R. L. Lemire, E. H. King, R. M. Hopper, C. O. Lemley, T. T. N. Dinh, and D. D. Burnett. 2017. Effect of maternal melatonin supplementation during mid to late gestation on fatty acid composition in maternal and fetal plasma and perirenal adipose tissue collected from bovine fetuses at 240 days of gestation. J. Anim. Sci. 95(Suppl4):152-152.
31. Holtcamp, A. J., A. T. Sukumaran, E. K. Wilkerson, A. E. Schnedler, B. J. McClenton, R. L. Lemire, C. R. Calkins, D. D. Burnett, and T. T. N. Dinh. 2017. Mitochondrial lipid composition and enzyme activity of post mortem beef longissimus muscle from Angus steers fed endophyte-infected tall fescue seeds. Reciprocal Meats Conference. AMSA2017-1152
32. Lemire, R. L., K. F. Coble, D. Garry, L. L. Thomas, C. W. Hastad, and D. D. Burnett. 2017. 127 Effect of double stocking and nursery split-out age on wean-to-finish growth performance and economic parameters of growing pigs. J. Anim. Sci. 95(Suppl2):60-60
33. McCarty, K. J., M. P. T. Owen, C. G. Hart, K. C. Yankey, R. C. Thompson, D. D. Burnett, E. H. King, R. M. Hopper, and C. O. Lemley. 2017. 487 Effect of melatonin supplementation during mid- to late-gestation on maternal uterine blood flow and calf size at birth. J. Anim. Sci. 95(Suppl4):238-238.
34. Yang, Z., M. S. Hasan, R. Thompson, M. A. Crenshaw, D. D. Burnett, J. K. Htoo, and F. Liao. 2017. Effects of dietary methionine deficiency on the growth performance and plasma concentrations of selected metabolites in growing pigs. J. Anim. Sci. 95 (Suppl. 4): 43. Abstract was presented by ZY at the ASAS-CSAS Annual Meeting & Trade Show, Baltimore, MD. Jul. 8-12.
35. Hasan, M. S., M. A. Crenshaw, J. M. Feugang, and F. Liao. 2017. Effects of dietary lysine restriction on the concentrations of free amino acids and other selected metabolites in the blood plasma of growing pigs. J. Anim. Sci. 95 (Suppl. 4): 49-50. Abstract was presented by MSH at the ASAS-CSAS Annual Meeting & Trade Show, Baltimore, MD. Jul. 8-12.
36. Wang, T., M. S. Hasan, M. A. Crenshaw, G. Wu, and F. Liao. 2017. Effects of dietary lysine supply on the plasma concentrations of growth-related hormones in late-stage finishing pigs. J. Anim. Sci. 95 (Suppl. 4): 205-206. Abstract was presented by MSH at the ASAS-CSAS Annual Meeting & Trade Show, Baltimore, MD. Jul. 8-12.
37. Humphrey, R. M., Z. Yang, M. S. Hasan, M. A. Crenshaw, D. D. Burnett, and F. Liao. 2017. The carcass characteristic shift in the compensatorily-gained pigs produced from feeding a methionine-deficient diet. Poster presentation by RMH at the Spring Undergraduate Research Symposium. p. 46. Shackouls Honors College, Mississippi State University. Apr. 13.
38. Durfey, C. L., F. Liao, D. Devost-Burnett, M. A. Crenshaw, C. S. Steadman, S. T. Willard, P. L. Ryan, H. Clemente, and J. M. Feugang. 2017. Assessment of growth and health performance of pigs born from magnetically nanopurified boar spermatozoa. J. Anim. Sci. 95 (Suppl. 2)/J. Dairy Sci. 100 (Suppl. 1): 40. Abstract was presented (oral and poster) by DCL the Annual Meeting of Midwestern Section/Branch of ASAS/ADSA, Omaha, NE. Mar. 13-15.
39. Wang, T., M. S. Hasan, M. A. Crenshaw, A. T. Sukumaran, T. Dinh, and F. Liao. 2017. Effect of dietary lysine on the skeletal muscle intramuscular fat content and fatty acid composition of late-stage finishing pigs. J. Anim. Sci. 95 (Suppl. 2)/J. Dairy Sci. 100 (Suppl. 1): 46-47. Abstract was presented (oral and poster) by MSH at the Annual Meeting of Midwestern Section/Branch of ASAS/ADSA, Omaha, NE. Mar. 13-15.
40. Hasan, M. S., T. Wang, S. Ching, J. M. Feugang, M. A. Crenshaw, L. S. Johnston, F. Chi, and F. Liao. 2017. Effect of a new montmorillonite-based feed additive on the production performance of newly weaned piglets. J. Anim. Sci. 95 (Suppl. 2)/J. Dairy Sci. 100 (Suppl. 1): 73-74. Abstract was presented (poster) by MSH the Annual Meeting of Midwestern Section/Branch of ASAS/ADSA, Omaha, NE. Mar. 13-15.
41. Moorhead, W. A., C. L. Durfey, F. Liao, D. Devost-Burnett, G. D. A. Gastal, P. L. Ryan, S. T. Willard, and J. M. Feugang. 2017. Effects of nanopurified boar semen for artificial insemination on protein detection in swine offspring muscle and fat tissue. Reproduction, Fertility and Development. 29(1): 139-139. Abstract was presentation (poster) by WAM at the 43^rd IETS Annual Meeting, Austin, TX. Jan. 14-17.
42. Durfey, C. L., F. Liao, D. Devost-Burnett, T. Dinh, M. Crenshaw, S. T. Willard, P. L. Ryan, H. Clemente, and J. M. Feugang. 2017. Growth and market quality of pigs born from magnetic nanoparticle treated boar spermatozoa. Abstract (#69016). Poster presentation by CLD at the 43^rd IETS Annual Meeting, Austin, TX. Jan. 14-17.

Theses:

1. Jones, A.K. 2017. The effect of poor maternal nutrition on offspring growth and maternal and offspring inflammatory status during gestation. PhD Diss. Univ. Connecticut, Storrs, CT.
2. Lisa C. Armbruster MS- Thesis August 2017: “Myogenic and Anabolic Gene Expression in Red and White Muscle from Sablefish (Anaplopoma fimbria) during Grow out”.
3. Breann N. Sandberg MS- Thesis May 2017: “ An Examination of the Effects of Dietary Rumen Protected- Histidine Supplementation on Finishing Beef Cattle Growth, Carcass and Meat Quality Parameters.
4. Elizabeth Hogan. The effects of virus-induced maternal inflammation on methylation patterns in skeletal muscle. MS Thesis December 2016.
5. Kellie Kroscher. Creation and characterization of mice with a mutation disrupting binding of a transcriptional repressor of insulin-like growth factor 2. MS Thesis August 2017

Other Publications and Presentations:

1. Selsby JT. The effect of heat stresses on porcine skeletal muscle.  Project Director’s meeting.  Baltimore, MD, 7/13/17.
2. Turning down the heat: How heat stress affects muscle growth and limits pork production. Iowa Swine Day, Ames, IA, 6/29/17.
3. Spaulding H, Kelly EM, Sheffield JB, Quindry JC, Hudson MB, and Selsby JT. Impaired autophagic flux in dystrophic muscle augments extracellular autophagosome release.  Advances in Skeletal Muscle Biology in Health and Disease.  Gainesville, FL, March 8-10, 2017. 

*Note: Talk was awarded based on abstract (1/16 selected from ~120 submitted)

4. Baumgard L., SK Kvidera, EA Horst, MJ Dickson, JA Ydstie, CS Shouse, EJ Mayorga, M Al-Qaisi, S Lei, KL Bidne, JT Seibert, BJ Hall, AF Keating, JW Ross, JT Selsby and RP Rhoads. Consequences of leaky gut on the immune system, metabolism, physiology and animal performance.  American Dairy Science Association. 
5. Spaulding HR and Selsby JT, Autophagic dysfunction in dystrophic muscle is independent of disease progression. Iowa Physiological Society, Des Moines, IA, October 29^th, 2016. 
6. The heat is on: Heat stress-mediated changes in skeletal muscle. Modern Views of Nutrition Seminar Series.  Iowa State University, 9/20/17.
7. It’s getting hot in here: Heat stress-mediated changes in skeletal muscle. TriBeta Seminar 4/4/17. 
8. Selsby, JT. Heat stress has effect on muscle growth, limits pork production.    September 5^th, p26-27, p32, 2017. 
9. Boosting Heat Stress Tolerance in Turkeys. In Retaking the Field, Vol. 2, March 2017. A publication of the Supporters of Agricultural Research (SoAR) Foundation. Available at: http://supportagresearch.org/michigan-state-university-boosting-heat-stress-tolerance-in-turkeys/ Accessed December 7, 2017.
10. Invited Speaker at Texas Tech University. K.J. Thornton. Heat shock proteins and their role in meat quality. March 2017.