SAES-422 Multistate Research Activity Accomplishments Report

Sections

Status: Approved

Basic Information

Participants

See attached list

NC246.annual-meeting-participants.docx

See attached file for NC246's meeting minutes.

NC246-Minutes-2016-meeting.pdf

Accomplishments

Participants presented research and extension data at over 150 meetings, and reaching over 5,000 participants [these are conservative estimates based on state reports and averages for extension faculty]

Chuck Mason’s Book: “EUROPEAN CORN BORER: ECOLOGY AND MANAGEMENT AND ASSOCIATION WITH OTHER CORN PESTS” co-authored with many NC-205 members (now members of NC246) was available as a PDF.

Dr. Chris DiFonzo published the latest update to the Handy Bt-Trait Table (http://www.msuent.com/assets/pdf/28BtTraitTable2016.pdf). This is a tool that many NC246 participants use for extension training and is an important general reference for industry, scientists and growers.

Participants developed various extension videos for corn insect scouting such as (Scouting for Corn Rootworm, Evaluating Corn Roots, Early-season below ground corn insect pests, Early-season corn insect pests, Mid-season corn insect pests, and Late-season corn insect pests).

Impacts

  1. Characterizing adult WCR dispersal from cornfields. Impact Statement: Ground- and at 10m-tower based sampling throughout the period of adult WCR emergence, revealed that at least 15% of newly-mated WCR females initiate long distance dispersal from their natal Bt-cornfields. Treating Bt-resistance remediation as a local, field-specific problem fails to acknowledge the contribution of regional-scale dispersal. WCR resistance management should not only focus on specific ‘problem’ fields.
  2. Evaluating the effect of foliar insecticides on populations of suspected Bt-resistant western corn rootworm beetles in rotated soybean Impact Statement: A single early or late season foliar insecticide treatment applied to rotated soybean fields did not reduce populations of egg-laying western corn rootworm adults. Demonstrating that adult WCR control in soybeans is ineffective will prevent unnecessary application of insecticides (chiefly pyrethroids) reduce selection on a chemistry that is one of the last alternative tactics available to growers threatened by rotation- and Bt-resistant WCR populations.
  3. Genetics of Bt-resistance in WCR Impact Statement: Transgenic maize varieties are an environmentally safe and valuable tool to control western corn rootworms. However, this pest, which causes more damage to corn than any other insect, has become resistant to certain transgenic varieties. A collaboration between Industry and academic researchers helped develop molecular markers for WCR and identified a single locus responsible for Bt resistance. These markers will be used in future field populations to determine the distribution and frequency of resistant rootworms.
  4. Insecticide Resistance in WCR Impact Statement: Western corn rootworm resistance to the pyrethroid insecticide bifenthrin was confirmed in SW Kansas and SW Nebraska. Ongoing studies will identify potential cross-resistance relationships and clarify how evolving resistance levels may contribute to rootworm injury in the field.
  5. Development of RNA-interference for WCR control: Impact Statement: The ability to deliver dsRNA in a trans-generational manner by feeding to adult rootworms was experimentally demonstrated. This approach does not kill adults but greatly reduces egg viability. The potential to develop parental RNAi technology targeting progeny of adult rootworms in combination with Bt proteins or dsRNA lethal to larvae may increase opportunities to develop sustainable approaches to rootworm management involving RNAi technologies for rootworm control.
  6. Best Management Practices for WCR control: Impact Statement: “Early detection and mitigation of resistance to Bt maize by western corn rootworm (Coleoptera: Chrysomelidae)” published in Journal of Economic Entomology and co-authored by NCCC46/NC246 members contributed formal recommendations about corn rootworm IRM policy and clarifies resistance-related terminology.
  7. Distribution and Management of Asiatic Garden Beetle: Impact Statement: AGBs are invasive grubs that are common in turfgrass, but have recently caused economic damage in corn. Grubs are more common in 2nd year corn, and earlier planted fields have a higher risk of damage. Trials with soil insecticides and seed treatments show mixed results, and likely provide control in low-pressure conditions. Research showed that AGB can be a significant pest if not properly managed.
  8. Resistance Alleles in Spodoptera frugiperda: Impact Statement: We determined the prevalence of Bt resistance in Spodoptera frugiperda. The conclusions that Cry2Ab and Vip3Aa can help to prevent resistance to Cry1F in Spodoptera frugiperda are important and need to be disseminated to seed producers as well as researchers focused on resistance development in the field.
  9. Corn Earworm life history on Bt-corn. Impact Statement: Estimations of biological characteristics of corn earworm on Bt maize are needed to detect the first instances of resistance. This is the first study to show differences among growth characteristics for Helicoverpa zea when collected from corn hybrids expressing Cry1Ab in the field. Furthermore, this is the first study to show a decrease in pupal weight for Helicoverpa zea when collected from corn hybrids expressing Cry1F in the field.

Publications

PUBLICATIONS (2015-2016)

2015

  1. Andow, D.A., S.G. Pueppke, A.W. Schaafsma, A.J. Gassman, T.W. Sappington, L.J. Meinke, P.D. Mitchell, T.M. Hurley, R.L. Hellmich, and R.P. Porter. 2015. Early Detection and Mitigation of Resistance to Bt Maize by Western Corn Rootworm (Coleoptera: Chrysomelidae). Journal of Economic Entomology Forthcoming.
  2. Cheng Y, Wang K, Sappington TW, Luo L, Jiang X. 2015. Response of reproductive traits and longevity of beet webworm to temperature, and implications for migration. Journal of Insect Science 15(1): 154.
  3. Chu, C.-C., J.A. Zavala, J.L. Spencer, M.J. Curzi, C.J. Fields, J. Drnevich, B.D. Siegfried and M.J. Seufferheld. 2015. Patterns of differential gene expression in adult rotation-resistant and wild-type western corn rootworm digestive tracts. Evolutionary Applications. 8(7):692-704.
  4. Cissel WJ, Mason CE, Whalen J, Hough-Goldstein J, Hooks CR.2015. Effects of Brown Marmorated Stink Bug (Hemiptera: Pentatomidae) Feeding Injury on Sweet Corn Yield and Quality. J Econ Entomol.
  5. Coates BS, Poelchau M, Childers C, Evans JD, Handler A, Guerrero F, Skoda S, Hopper K, Wintermantel WM, Ling KS, Hunter WB, Oppert B, Perez de Leon AA, Hackett K, Shoemaker D. 2015. Arthropod genomics research in the United States Department of Agriculture-Agricultural Research Service:  Current impacts and future prospects.  Trends in Entomology 11:1-27.
  6. Coates BS, Siegfried BD. 2015. Linkage of an ABC transporter to a single QTL that controls Ostrinia nubilalis larval resistance to the Bacillus thuringiensis Cry1Fa toxin. Insect Biochem Mol Biol 63: 86-96.
  7. Coates BS.  2015. Horizontal transfer of a non-autonomous Helitron among insect and viral genomes. BMC Genomics 16: 137.
  8. Da Silva K.F., Spencer T.A., Camargo Gil C., Siegfried B.D., and. F.S. Walters. 2015Impact of Spodoptera frugiperda Neonate Pretreatment Conditions on Vip3Aa19 Insecticidal Protein Activity and  Laboratory Bioassay Variation. Pest. Manag. Sci. DOI: 10.1002/ps.4175.
  9. Dangal V. and F. Huang. 2015. Fitness costs of Cry1F resistance in two populations of fall armyworm, Spodoptera frugiperda (J.E. Smith), collected from Puerto Rico and Florida. J. Invertebr. Pathol. 127:81-86.
  10. Flagel LE, Swarup S, Chen M, Bauer C, Wanjugi H, Carroll M, Hill P, Tuscan M, Bansal R, Flannagan R, Clark TL, Michel AP, Head GP, Goldman BS. 2015. Genetic markers for western corn rootworm resistance to Bt toxin. G3: Genes | Genomes | Genetics. 5(3):399-405.
  11. Frank, D.L., R. Kurtz, N.A. Tinsley, A.J. Gassmann, L.J. Meinke, D. Mollenbeck, M.E. Gray, L.W. Bledsoe, C.H. Krupke, R.E. Estes, P. Weber and B.E. Hibbard.  2015. Effect of Seed Blends and Soil-Insecticide on Western and Northern Corn Rootworm Emergence from mCry3A+eCry3.1Ab Bt Maize. Journal of Economic Entomology. 108:1260-1270. DOI: 10.1093/jee/tov081.
  12. Gaspar, A.P., P.D. Mitchell, and S.P. Conley. 2015. Economic Risk and Profitability of Soybean Seed Treatments at Reduced Seeding Rates. Crop Science 55:924-933.
  13. Gray, M.E. and J.L. Spencer. 2015. Western corn rootworm: Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) resistance to Bt maize and crop rotation: management challenges and opportunities. Bulletin of the Royal Entomological Society; Antenna: ECE Special Edition. 39:100-101.
  14. Hanson, A. A., R. D. Moon, R. J. Wright, T. E. Hunt, and W. D. Hutchison. 2015. Degree-day prediction models for the flight phenology of western bean cutworm (Lepidoptera: Noctuidae) assessed with the concordance correlation coefficient. J. Econ. Entomol. 1–11
  15. Hitchon AJ, Smith JL, French BW, Schaafsma AW. 2015. Impact of the Bt Corn Proteins Cry34/35Ab1 and Cry3Bb1, Alone or Pyramided, on Western Corn Rootworm (Coleoptera: Chrysomelidae) Beetle Emergence in the Field. J Econ Entomol.
  16. Huang, F., M. Chen, A. Gowdab, T.L. Clark, B.C. McNulty, F. Yang, and Y. Niu. 2015. Identification, inheritance, and fitness costs of Cry2Ab2 resistance in a field-derived population of sugarcane borer, Diatraea saccharalis (F.). J. Invertebr. Pathol. 130: 116.
  17. Hughson, S.A. and J.L. Spencer. 2015. Emergence and abundance of western corn rootworm (Coleoptera: Chrysomelidae) in Bt cornfields with structured and seed blend refuges. J. of Economic Entomology. 108(1):114-125. doi: 10.1093/jee/tou029. Article first published online: 21 JAN
  18. Hurley, T., and P. Mitchell. Value of Insect Pest Management to U.S. and Canadian Corn, Soybean and Canola Farmers. AAEA Conference Paper, July 2015. Online: http://ageconsearch.umn.edu/bitstream/205129/2/AAEA%20Neonic%20Selected%20Paper%202015.pdf.
  19. Khajuria C., Vélez A.M., Rangasamy M., Wang H., Fishilevich E., Frey M.L.F., Portilho Carneiro N., Gandra P., Narva K.E. and B.D. Siegfried.  2015. Parental RNA interference of genes involved in embryonic development of the western corn rootworm, Diabrotica virgifera virgifera LeConte. Insect Biochemistry and Molecular Biology. 63: 54-62.
  20. Levy RC, Kozak GM, Wadsworth C, Coates BS, Dopman EB.  2015. Explaining the sawtooth: Latitudinal periodicity in a circadian gene correlates with shifts in generation number. Journal of Evolutionary Biology 28(1):40-53.
  21. Li H., Khajuria C., Rangasamy M., Gandra P., Fitter M., Geng C., Woosely A., Hasler J., Schulenberg G., Worden S., McEwan R., Evans C., Siegfried B. and K.E. Narva. 2015.  Long dsRNA but not siRNA initiates RNAi in western corn rootworm larva and adults. J. Appl. Entomol. 139: 432-445.
  22. Mitchell, P.D.  2015.  An Economic Assessment of the Benefits of Nitroguanidine Neonicotinoid Insecticides in the United States and Canada.  AgInfomatics Research Report, Madison, WI (35 p).  Online: http://growingmatters.org/studies/.
  23. Pannuti, L.E.R., E.L.L. Baldin, T. E. Hunt, and S. V. Paula-Moraes. 2015. On-Plant Larval Movement and Feeding Behavior of Fall Armyworm (Lepidoptera: Noctuidae) on Reproductive Corn Stages. Environmental Entomology,
  24. Pereira A., Wang H., Zukoff S., Meinke L.J., French B.W. and B.D. Siegfried. 2015. Evidence of Field-Evolved Resistance to Bifenthrin in Western Corn Rootworm (Diabrotica virgifera virgifera LeConte) Populations in Western Nebraska and Kansas. PLoS ONE. 10(11): e0142299. doi:10.1371/journal.pone.0142299.
  25. Reisig, D. D., and F. P. F. Reay-Jones. 2015. Inhibition of Helicoverpa zea growth by transgenic corn expressing Bt toxins and development of resistance to Cry1Ab. Environ. Entomol. 44(4):1275-85.
  26. Reisig, D. D., D. S. Akin, R. T. Bessin, M. J. Brewer, D. G. Buntin, A. L. Catchot, D. Cook, K. L. Flanders, F.- N. Huang, D. W. Johnson, B. R. Leonard, P. J. McLeod, R. P. Porter, F.P.F. Reay-Jones, K. V. Tindall, S. D. Stewart, N. N. Troxclair, R. R. Youngman, and M. E. Rice. 2015. Lepidoptera (Crambidae, Noctuidae, and Pyralidae) injury on corn containing pure single and pyramided Bt traits and non-Bt hybrids compared to a refuge blend with non-Bt and pyramided Bt hybrids, in the southern United States. J. Econ. Entomol. 108: 157-165.
  27. Schaafsma A, Limay-Rios V, Baute T, Smith J, Xue Y (2015) Neonicotinoid Insecticide Residues in Surface Water and Soil Associated with Commercial Maize (Corn) Fields in Southwestern Ontario. PLoS ONE 10(2): e0118139.
  28. Smith JL, Baute TS, Mason CE 2015. Pheromone races of Ostrinia nubilalis Hübner (Lepidoptera: Crambidae) infesting grain corn in Manitoba, Ontario, and Québec provinces of Canada. Journal of the Entomological Society of Ontario. 146: 41-49.
  29. Tabashnik, B.E., M. Zhang, J.A. Fabrick, Y. Wu, M. Gao, F. Huang, J. Wei1, J. Zhang, A. Yelich, G.C. Unnithan, A. Bravo, M. Soberón, Y. Carrière, and X. Li. 2015. Dual mode of action of Bt proteins: protoxin efficacy against resistant insects. Scientific Reports. 5:15107, DOL:10.1038/srep15107.
  30. Tinsley N.A., Estes R.E., Schrader P.M., and M.E. Gray. 2015. Evaluating multiple approaches for managing western corn rootworm larvae with seed blends. J. Appl. Entomol. 139: 76–86 (DOI: 10.1111/JEN.12134).
  31. Tinsley, N.A., J.L. Spencer, R.E. Estes, J.R. Prasifka, P.M. Schrader, B.W. French, and M.E. Gray. 2015. Larval mortality and development for rotation-resistant and rotation-susceptible populations of western corn rootworm on Bt corn.  J. of Applied Entomology. 139:46-54. Article first published online 21 JUL 2014.  doi: 10.1111/jen.12149.
  32. Tinsley, N.A., P.D. Mitchell, R.J. Wright, L.J. Meinke, R.E. Estes and M.E. Gray (in press). Estimation of efficacy functions for products used to manage corn rootworm larval injury. Journal of Applied Entomology. Article first published online: 18 NOV 2015.  DOI: 10.1111/jen.12276
  33. Vellichirammal NN, Wang H, Eyun S, Moriyama E, Coates BS, Miller NJ, Siegfried BD. 2015Transcriptional analysis of susceptible and resistant European corn borer strains and their response to Cry1F protoxin. BMC Genomics 16: 558.
  34. Wangila, D. S., A. J. Gassmann, J. L. Petzold-Maxwell, B. W. French, and L. J. Meinke. 2015. Susceptibility of Nebraska western corn rootworm (Coleoptera: Chrysomelidae) populations to Bt corn events.  J. Econ. Entomol. 108: 742-751.
  35. Xue Y, Limay-Rios V, Smith J, Baute T, Forero LG, Schaafsma A. 2015. Quantifying Neonicotinoid Insecticide Residues Escaping during Maize Planting with Vacuum Planters. Environ Sci Technol.
  36. Yang, F., D. Kerns, and F. Huang. 2015. Refuge-in-the-bag strategy for managing insect resistance to Bt maize. Outlook. Pest manag. 26: 226-228.
  37. Yang, F., D.L. Kerns, B.R. Leonard, I. Oyediran, T. Burd, Y. Niu, and F. Huang. 2015. Performance of Agrisure®VipteraTM3111 corn againstHelicoverpa zea (Lepidoptera: Noctuidae) in seed mixed plantings. Crop Prot. 69: 77-82.
  38. Zhang TT, Coates BS, Ge X, Bai S, He K, Wang Z. 2015. Male- and female-biased gene expression of olfactory related genes in the antennae of the Asian corn borer (Guenée) (Lepidoptera: Crambidae). PLoS One 10(6):e0128550.
  39. Zhu, Y-C., C.A. Blanco, M. Portilla, J. Adamczyk, R. Luttrell, and F. Huang. 2015. Evidence of multiple/cross resistance development to Bt and organophosphate insecticides in Puerto Rico population of the fall armyworm, Spodoptera frugiperda. Pesti. Biochem. Physiol. 122:15-21.

2016:

  1. Coates BS, Alves A, Wang H, Zhou X, Nowatski T, Chen H, Rangasamy M, Robertson HM, Whitfield CW, Walden KK, Kachman SD, French BW, Meinke LJ, Hawethorne D, Abel CA, Sappington TW, Seigfried BD, Miller NJ. 2016. Genetic mapping and functional genomics link a carboxyesterase with organophosphate resistance in the western corn rootworm, Diabrotica virgifera virgifera. Insect Molecular Biology 25(1):1-15.
  2. Estes, R.E., N.A. Tinsley and M.E. Gray. 2016. Evaluation of soil-applied insecticides with Bt maize for managing corn rootworm larval injury. Journal of Applied Entomology. 140:19-27. doi: 10.1111/jen.12233
  3. Huesing, J.E., Andres, D., Braverman, M.P., Burns, A., Felsot A. S., Harrigan, G.G., Hellmich, R.L., Reynolds, A., Shelton, A.M., Jansen van Rijssen, W., Morris, E.J., and Eloff, J. N. 2016. Global Adoption of Genetically Modified (GM) Crops: Challenges for the Public Sector. Journal of Agriculture and Food Chemistry, DOI: 10.1021/acs.jafc.5b05116.
  4. Li G, Reisig D, Miao J, Gould F, Huang F, Feng H (2016) Frequency of Cry1F Non-Recessive Resistance Alleles in North Carolina Field Populations of Spodoptera frugiperda (Lepidoptera: Noctuidae). PLoS ONE 11(4): e0154492.
  5. Limay-Rios, V., Forero, L. G., Xue, Y., Smith, J., Baute, T. and Schaafsma, A. (2016), Neonicotinoid insecticide residues in soil dust and associated parent soil in fields with a history of seed treatment use on crops in southwestern Ontario. Environ Toxicol Chem, 35: 303–310.
  6. Oyedirana, I., G. Dively, F. Huang, and T. Burd. 2016. Evaluation of European corn borer Ostrinia nubilalis (Lepidoptera: Crambidae) larval movement and survival in structured and seed blend refuge plantings. Crop Prot. 81:145-153.
  7. Poelchau M, Coates BS, Childers CP, Perez de Leon AA, Evans JD, Hackett K, Shoemaker D. 2016. Agricultural applications of insect ecological genomics. Current Opinions in Insect Science 13:61-69.
  8. Schaafsma, A., Limay-Rios, V., Xue, Y., Smith, J. and Baute, T. (2016), Field-scale examination of neonicotinoid insecticide persistence in soil as a result of seed treatment use in commercial maize (corn) fields in southwestern Ontario. Environ Toxicol Chem, 35: 295–302.
  9. Tinsley, N., P. Mitchell, R. Wright, L. Meinke, R. Estes, M. Gray. 2016. Estimation of Efficacy Functions for Products Used to Manage Corn Rootworm Larval Injury.  Journal of Applied Entomology (in press).
  10. Vélez A.M., Jursenski J., Matz N., Zhou, J. Wang H., Ellis M., and B.D. Siegfried. 2016. Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L. to dietary RNAi. Chemosphere. 144: 1083-1090.
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