Brief Summary of Minutes of Annual Meeting

The meeting was called to order 10:50 am by Julie Pashe, Chair, W-3150. Julie Pasche welcomed everyone and introduced Khwaja Hossain as Vice Chair. Jim Kelly made a motion to elect Rubella Goswami as the Secretary and the motion was 2^nd by Phil McClean. The motion passed with all in favor and Rubella Goswami started serving as secretary immediately. This need was brought about by the inability of the secretary elect (Vicki Schelgal, U of NE) to attend this meeting. Dr. Schegal will be nominated to serve as secretary in 2016.

A motion was made by Juan Osorno and seconded by Phil Miklas to approve the minutes of the previous meeting. Introductions of attendees followed.

Janice Rueda, Past Chair, reported that the submission for the 5 year renewal of the W2150 (now W3150) had gone smoothly and thanked members for their inputs.

Julie Pasche, informed the group that the minutes for the meeting along with the State Reports had to be submitted within 60 days from the date of the meeting and requested each state representative to send their reports to any of the office bearers. The length of the report was limited to 1-1.5 pages.

Dr. Mike Harring provided administrative update via phone. Dr. Harring’s comments included the possibility of an increase in budget and AFRI funds, changes in the IPM program and creation of an agriculture research institute. There were no questions from the attendees.

Qijian Song, USDA-ARS, Beltsville, MD presented “Development of SNP BeadChips in Common Bean”. Using a set of 17 diverse accessions from major market classes, nearly 2 million SNPs were identified. A series of 3 Bead chips have been designed and used to identify genes or QTL associated with resistance to bean common mosaic virus, root rot, rust, bacterial blight and leaf hopper as well as root architecture, drought and multiple stress tolerance. He also discussed the availability of the Soybean Bead Chip available through the BARCSoy6K BeadChip Consortium, where additional SNPs can be added to existing SNPs. His presentation was followed by a discussion that Bead Chips may be a better option than GBS as it reduces the need for bioinformatics and results are delivered directly in a spread-sheet.

State reports were given. See meeting minutes for state reports.

Major activities completed:

• Development and release of bean cultivars ‘Beniquez’ and ‘Badillo’ and improved bean germplasm TARS LFR1, PR0806-80, PR0806-81, PR0401-259, PR0650-31, TARS-MST1 and SB-DT1.
• Identification of genes for resistance to common bacterial blight.
• Characterization of the virulence patterns of isolates of the angular leaf spot and ashy stem blight and common bacterial blight pathogens.
• The project made progress toward all of the specific objectives:
• Conduct a bean breeding program by crossing promising parents and selecting breeding lines for adaptation, agronomic traits and disease resistance and evaluate the performance of advanced generation breeding lines on experiment stations and farms
• Study the inheritance of resistance to common bean diseases,
• Isolate and characterize bean pathogens in Puerto Rico
• Provide a winter nursery service for U.S. bean breeding programs

Tim Porch, USDA-ARS-TARS, Mayaguez, PR

Breeding lines developed from a second cycle of recurrent selection for drought in the collaborative shuttle breeding with the U. of Nebraska were evaluated in Nebraska and in Puerto Rico in 2015. In collaboration with USDA-ARS-Prosser, over 200 bulk breeding populations have been developed for abiotic and biotic traits in Mesoamerican and Andean genetic backgrounds and are freely available. A diversity analysis of angular leaf spot isolates from Central America, Puerto Rico, and Tanzania is being conducted through sequencing of specific loci in collaboration with the U. of Puerto Rico. Evaluation methods, virulence analysis, and the genetics of the response to ashy stem blight is being conducted on both a RIL population and on the panel with the U. of Puerto Rico. GWAS analysis is being conducted on a number of abiotic stress traits in the ADP and BASE120 panels and in a Mesoamerican RIL population.

A tepary diversity panel (TDP) was developed and genotyped using genotyping-by-sequencing. The TDP was evaluated for response to bean common mosaic virus and biological nitrogen fixation, and a small set of accessions were identified with BCMV resistance. Agronomic traits were evaluated in Puerto Rico, Arizona, and Colorado. In addition, advanced lines of tepary (Phaseolus acutifolius) in a tepary adaptation trial (TAT) were generated, and are currently being tested at Colorado State through a shuttle breeding effort, and in Central America.

A database of the Andean Diversity Panel (ADP), and SNP genotypic information on the ADP generated through genotyping-by-sequencing are being made available for use through the FtF-ARS Grain Legumes Project website http://arsftfbean.uprm.edu/bean/.

Washington

Theodore Kisha, Giuliana Naratto

Dry bean nutrient analysis and characterization of exotic germplasm research at Washington State University in 2015 are summarized below:

Nutrient analysis of selected dry beans: Of the more than 20,000 Phaseolus accessions held at the Western Regional Plant Introduction Station (WRPIS), the most abundant species by far is P. vulgaris L. with over 17,000 accessions. Of these, 177 are described as “snap” varieties, grown for harvest as fresh vegetable, while the remainder are described as “dry beans”. Among these, 90 are classified as nuña beans, or the Peruvian “popping” bean. These beans have been selected and raised among the Andean natives in the high mountains for millennia and have the unique characteristic of bursting when subjected to heat, making them a high protein food in conditions where boiling would consume scarce fuel. This property also makes these beans a potential nutritious snack food, both in and of itself, as well as in the form of an extruded product. We analyzed the molecular diversity of 35 nuña and 8 common dry bean accessions and compared a range of nutritional factors, including protein, starch, sugars, phytate, and antioxidant activity. Genetic analysis using AFLP markers showed nuñas were distinct from the common dry beans analyzed, and there were two distinct groups within the nuñas. There was a similar wide range of nutritional characteristics within both the common dry beans and the nuñas. Values for nuñas and common bean respectively were: protein (18-25 and 17-27%), extractable polyphenols (50-350 and 50-450 mg GAE/100g), non-extractable polyphenols (50-220 and 70-175 mg GAE/100g), phytate (0.45-1.2 and 0.6-1.0%), and total antioxidant activity (8-52 and 7-48 mgTE). There is enough genetic variation in both nuña and common dry beans to breed popping beans adapted to a temperate, long-day environment and to develop a highly nutritious snack food for America.

Genetic Diversity of North American wild kidney bean (Phaseolus polystachios) in the eastern United States: North American wild kidney bean or thicket bean (Phaseolus polystachios (L.) Britton, Sterns, & Poggenb.) is a perennial vine found in the eastern United States from Texas to Connecticut. Habitat destruction and urbanization are limiting its distribution: e.g., it was once prevalent in the Detroit River International Wildlife Refuge, but has not been seen there since the late 1800’s. Crop wild relatives are a critical source of genetic diversity, often holding untapped genes for breeding of domesticated plants in agriculture for disease resistance, yield, quality, and adaptation to climate change, as well as ecologically important members of natural habitat. The closest cultivated relative of P. polystachios is P. lunatus, the lima bean. Through coevolution in its natural habitat, P. polystachios may have acquired true resistance to the ubiquitous pathogen white mold (Sclerotinia sclerotiorum) and provide a source for interspecific transfer. The Western Regional Plant Introduction Station of the National Plant Germplasm System holds over 17,000 accessions of Phaseolus from 47 species groups, but has only 10 accessions of the wild Phaseolus polystachios, 5 of which were only recently collected in Florida. Understanding genetic diversity is critical for identifying areas to target for recovering maximum genetic representation. Molecular markers are an important tool for analyzing the extent and distribution of genetic diversity within and among wild populations and are important for identifying geographic gaps for collecting underrepresented genotypes. We analyzed nine accessions from the USDA collection along with sixteen herbarium samples provided by the Smithsonian Institution using 231 AFLP molecular markers from six primer combinations. While the DNA from the herbarium samples was somewhat degraded, markers at and below 200 bp were readily discernible and showed four distinct clusters. One herbarium sample from Florida was distinct from the others and, because of the lobed leaves, is likely P. smilacifolius. The USDA accession from Texas was very unique and has been reclassified as P. texensis. The level of distinction among the samples studied here reinforces the need for continued collection of this diverse species. A collection expedition was carried out in October and additional populations were collected in Ohio, Indiana, West Virginia, and Missouri.

Nebraska

Jim Steadman and Carlos A. Urrea

Root, stem and crown rots are increasingly becoming a yield constraint to dry bean production. The major soil-borne pathogens we have found associated with root, stem and crown rots include Fusarium solani, Fusarium oxysporum, Pythium ultimum, Rhizoctonia solani and Macrophomina phaseolina. Identification of the major pathogens causing root rots helps direct breeding program efforts for disease resistance. Symptomatic bean samples were collected from bean fields in Nebraska and Puerto Rico. Traditional fungal isolation and molecular and morphological identification and sequencing DNA from root tissue guided fungal genus and species information. Fusarium species were detected in 82% of DNA sequenced from all Nebraska root samples and 83% of the isolated pathogenic fungi. Nearly 50% of the isolates from Puerto Rico were Macrophomina phaseolina. Both DNA analysis with species specific primers and sequencing of pathogenic isolates identified Fusarium spp. as the most common pathogens in Nebraska and Macrophomina phaseolina to be more dominant in Puerto Rico.

The main pathogens reported to cause root and crown rots of dry bean are Fusarium solani, Fusarium oxysporum, Pythium ultimum, Rhizoctonia solani and Macrophomina phaseolina. Determining pathogenicity of putative causal agents in the root and crown rot complex is required but no simple tests were found in the literature. We compared 4 previously published fungal aggressiveness tests: detached leaf, stem, cup and straw and found significant differences among the pathogenicity testing methods (P<0.001 at 0.05 significance). The straw test had the highest disease incidence (100%) and highest mean disease damage score (5.8 ± 1.87 SD on the CIAT 1 – 9 scale) for all the tested pathogens. The straw test can be used as an easy and inexpensive method to separate pathogenic from non-pathogenic isolates for the major fungal and oomycete root pathogens of dry bean.

Multisite screening was used to identify and verify partial resistance to white mold in common bean. There were 6 field tests conducted in 6 locations testing 7 lines. In the field tests, all 7 lines were significantly more resistant than Beryl. The results of the 4 field tests reported were that 3 bean lines, GN 031-A-11, pinto USPT-WM-12 and snap bean ASR 1002 were similar to the resistant check G122 with intermediate resistance while black B10244 and red R12859 were similar to Bunsi. Navy N11283 and GN G12901 were less susceptible than Beryl. The greenhouse trials tested 11 entries, plus 3 controls, using the straw test on 21- to 28-day-old G122 bean plants. The greenhouse results indicate that 3 bean lines had ratings similar to G122 including 031-A-11 and USPT-WM-12 while 7 lines had ratings similar to Bunsi; however, greenhouse conditions are move favorable and allow the fungus to grow in optimal conditions which is less likely to be encountered in the field. All field entries including pinto, great northern, black, navy and cranberry seed classes were rated lower in susceptibility than Beryl. Progress in incorporating WM resistance into dry bean lines with commercial potential validates use of multisite screening

The 2015 evaluation of NE great northern and pinto lines with the rust pathogen under field conditions was conducted at Beltsville, MD. Almost all of the NE lines in the pinto nursery were resistant to the prevalent races of rust. However, the great northern nursery had several entries with intermediate rust resistance. One GN entry had a susceptible reaction. As in previous years, the spreader rows were inoculated with five races of the rust pathogen: 38, 39, 40, 41, and 43.

Coordinated, participated in, and distributed the regional WRBT trial planted at CO, ID, WA, and NE. Participated in the regional MRPN trial planted at ND, MI, CO, and NE. Contributed two great northern and two Nebraska pinto bean lines to both trials. Coordinated, participated in, and distributed the DBDN. Most of the DBDN lines are from the Shuttle Breeding between NE and PR. This trial was conducted in CO, NE, MI, and is being planted in PR.

The second generation of dry bean lines from the Shuttle Breeding between NE and PR was tested in 2015 under drought stress and non-stress conditions. Irrigation was stopped at flowering stage (terminal stress). Lines from the first cycle of Shuttle Breeding were used as reference checks. Data are being complied and analyzed. This summer a set of drought tolerant lines from previous years were screened for heat tolerance. Data are being analyzed.

In the next few days a great northern line will be released as a cultivar based on its performance in Nebraska since 2010. A set of elite great northern/pinto lines have been tested in growers’ fields under the ‘Mother and Baby’ Trial scheme. Data from these trials, the regional trials described above, and disease screening trials are being compiled. Data from trials evaluating the yield of different market classes (great northern, pinto, reds, blacks, light red kidney, and cranberries) are being analyzed. Several lines within each market class appeared to perform better than the reference checks.

Three bacterial wilt RILs were advanced to F2:3 through single seed descent. We will continue selfing the RILs until F4:5.

Wisconsin

James Nienhuis, Dept. of Horticulture, University of Wisconsin-Madison

Understanding and improving flavor in beans : screening the USDA Phaseolus core collection for pod sugar and flavor compounds in snap and dry bean accessions

The objective of our W3150 research is to gain knowledge regarding variation in sugar and flavor content among a sample of dry bean and green pod-type PI accessions from the USDA Phaseolus Germplasm Core Collection, Pullman, WA. Knowledge of the variation will allow better utilization of germplasm resources in the development of new bean cultivars with more desirable sugar and flavor profiles. The results of this project could be used to market product quality and offer unique opportunities to expand market share to an increasingly health conscious population.

Dr. Kisha USDA-ARS, Pullman, WA developed a diverse sub-core of 94 Plant Introductions (PI) characterized as snap beans, Romano-types, and other beans eaten as edible immature pods, and 20 dry bean PI accessions. In addition checks included a kidney bean (Montcalm, Andean gene pool) as well as 8 cultivars (e.g. Caprice, Huntington, 04-88, OSU5402, OSU5630, Masai, Slenderpack, Tapia) representing the various market classes consumed as edible green pods currently grown commercially in the United States.

A large positive correlation (r=0.79**)was observed between the simple sugars Glucose and Fructose. In contrast a large negative correlation was observed beweeen the disaccharide sucrose with both monosaccharides, glucose (r=-0.37) and fructose (r=-0.43). Glucose concentration had a mean of 19.96 mg g-1 dry weight, and ranged from near zero to over 40mg g^-1 dry weight. P.I accessions with high concentrations of sucrose were generally both heirloom and modern commercial snap beans cultivars, e.g. Provider, Eagle, Cascade, Hystyle and BBL47.   Fructose concentration had a mean of 19.9 mg g-1 dry weight, and ranged from near zero to over 50mg g^-1 dry weight. Sucrose had a much lower concentration of 3.7 mg g-1 dry weight, and ranged from near zero to over 14 mg g^-1 dry weight.

Michigan

James D. Kelly and Karen A. Cichy

Plant, Soil and Microbial Sciences, USDA-ARS, Michigan State University, East Lansing MI 48824

Bean Breeding Nurseries

The MSU dry bean breeding and genetics program conducted 12 yield trials in 2015 in ten market classes and participated in the growing and evaluation of the Cooperative Dry Bean, Midwest Regional Performance, National Drought and the National Sclerotinia Nurseries in Michigan and winter nursery in Puerto Rico. All yield trials at Frankenmuth were direct harvested. Large-seeded kidney and cranberry trials, at Montcalm were rod-pulled. The white mold trial was direct harvested. Temperatures were moderate for the 2015 season and only exceeding 90F for a few days in July. Overall rainfall for the 3-summer months at the Saginaw Valley Research and Extension Center (SVREC) was equivalent to the 30-year average of 8.5”. A moderate dry period occurred from June 16-July 13 with only 0.7” of rainfall which reduced the overall plant size and resulted in lower overall yields. A high incidence of common bacterial blight resulted in the nurseries and allowed for selection of resistant lines in a range of seed types. Rainfall patterns at the Montcalm Research Farm (MRF) were more extreme with a total rainfall of over 5” within two days of planting. This resulted in major flooding in some areas, soil crusting and compaction in other areas which resulted in low germination. In addition soil temperatures remained low in this critical period and a high incidence of root rots diseases occurred which also reduced germination and stands. The Andean kidney and cranberry beans were the most affected by the stresses whereas the Mesoamerican small and medium seeded black, navy, pinto, GN, and red beans managed to tolerate the conditions and had near normal stands. Overall vigor of the kidney and cranberry beans was poor resulting in small plants that had low overall yields. Plots at MRF had supplemental irrigation did contribute to the development of white mold. Incidence in the National Sclerotinia Initiative nursery was very low in the susceptible checks despite the overall lower temperatures and excess irrigation. The major problem at MRF was the presence of severe root rots mainly Fusarium that was accentuated by the cooler soil conditions early in the season. The unfavorable condition allowed for the selection of lines with tolerance to root rot and with resistance to common bacterial blight in the kidney bean nurseries.

Black Bean Color Retention

Color retention after canning is a major concern for the bean canning industry. Significant genetic variability exits and a molecular marker for color retention would be very useful to breeders. A panel of 71 black bean breeding lines was compiled from the major public U.S. black bean breeding programs, including Colorado State University, Michigan State University, North Dakota State University, University of Nebraska, USDA-ARS in East Lansing, MI, Mayaguez, Puerto Rico, and Prosser, Washington. These lines were grown in replicated field trials at the SVREC, Richville, MI in 2013 and 2014.   Each year beans were canned and evaluated for canning quality and color retention. Anthocyanins were also measured on raw and canned samples. The variability for color retention in the panel ranged from a low of 1.75 to a high of 4.75. These values are based on a scale of 1 to 5, where 1 is light brown and 5 is dark black. The ratings were given by a sensory panel of ~20 individuals. Each of the bean lines were genotyped with the BARCBean6K_3 SNP array of 5,398 SNP markers. In total, 2,799 SNP markers were polymorphic. The phenotypic and genotypic information was used for genome wide association analysis.   Genomic regions associated with color retention were found on chromosomes Pv02, Pv03, Pv04, Pv05, Pv06, Pv09, and Pv11.

Astudillo-Reyes, C., A.C. Fernandez, K.A. Cichy. 2015. Transcriptome Characterization of developing bean (Phaseolus vulgaris L.) pods from two genotypes with contrasting seed zinc concentrations. PLoS ONE 10(9): e0137157. doi:10.1371/journal.pone.0137157.

Beaver, J.S., J.C. Rosas, T.G. Porch, M.A. Pastor-Corrales, G. Godoy-Lutz and E.H. Prophete. 2015. Registration of PR0806-80 and PR0806-81 white bean germplasm with resistance to BGYMV, BCMV, BCMNV and rust. J. Plant Reg. 9:208-211.

Beaver, J.S., G. Godoy-Lutz, J.R. Steadman and T.G. Porch. 2011. Release of ‘Beníquez’ white bean (Phaseolus vulgaris L.) cultivar. J. of Agric. of the Univ. of Puerto Rico. 95:237-240.

Beaver, J.C., E.H. Prophete, J.C. Rosas, G. Godoy-Lutz, J.R. Steadman and T.G. Porch. 2014. Release of ‘XRAV-40-4’ black bean (Phaseolus vulgaris L.) Cultivar. J. of Agric. of the Univ. of Puerto Rico. 98:83-87.

Berry, M., K.A. Cichy, Y. Ai, and P.K.W. Ng. 2015. Phytoheamagglutination activity in extruded dry bean powder. Ann. Rep. Bean Improv. Coop. 58:1-2.

Burt, A.J., H. M. William, G. Perry, R. Khanal, K. P. Pauls, J. D. Kelly, A. Navabi. 2015. Candidate gene identification with SNP marker-based fine mapping of anthracnose resistance gene Co-4 in common bean. PLoS ONE 10(10): e0139450. doi:10.1371/journal.pone.0139450.

Estevez de Jensen, C., A. Vargas, T.G. Porch, and J.S. Beaver. 2014. Evaluation of virulence of different isolates of Macrophomina phaseolina in common bean using two inoculation methods. Bean Improv. Coop. 57:227-228.

Cichy, K.A., T.G. Porch, J.S. Beaver, P. Cregan, D. Fourie, R.P. Glahn, M.A. Grusak, K. Kamfwa, D.N. Katuuramu, P. McClean, E. Mndolwa, S. Nchimbi-Msolla, M.A. Pastor-Corrales and P.N. Miklas. 2015. A Phaseolus vulgaris diversity panel for Andean bean improvement. Crop Science 55:2149-2160. doi:10.2135/cropsci2014.09.0653.

Cichy, K.A., J.A. Wiesinger, and F.A. Mendoza. 2015. Genetic diversity and genome wide association analysis of cooking time in dry bean (Phaseolus vulgaris L.). Theoretical and Applied Genetics 128:1555-1567.

Ghising, K., J. Vasquez-Guzman, S. Schroder, A. Soltani, S.M. Moghaddam, S. Mamidi, P. McClean, J. M. Osorno, K. McPhee, J. Pasche, and R. Lamppa. 2015. Genome-wide approaches for identification of genomic regions associated with halo blight resistance in the USDA core collection of common bean. Presented at: Annu. Meet. American Society of Agron.-Crop Sci. Society of America, Soil Sci. Society of America (ASA-CSSA-SSSA); Nov. 15-18; Minneapolis, MN.

Ghising, K., J. Vasquez-Guzman, S. Schroder, A. Soltani, S.M. Moghaddam, S. Mamidi, P. McClean, J. M. Osorno, K. McPhee, J. Pasche, and R. Lamppa. 2015. Identifying genomic regions associated with halo blight resistance within the USDA core collection of common bean. Presented at: Bean Improv. Coop. Biennial Meeting; Nov. 2-4; Niagara Falls, Ontario, Canada.

Guachambala Cando, M.S., M. Zapata, J.S. Beaver and T.G. Porch. 2014. Inheritance of high levels of resistance to common bacterial blight caused by Xanthomonas axonopodis pv. phaseoli in common bean. Ann. Rep. Bean Improv. Coop. 57:179-180.

Halvorson, J., R.S. Lamppa, and J.S. Pasche. 2015. Characterization of Colletotrichum lindemuthianum races infecting dry edible bean in North Dakota. Canadian J. Plant Path. (Accepted 11/2/2015).

Halvorson, J.M., K. Simons, R.L. Conner, and J.S. Pasche. 2015. Seed-to-seedling transmission of Colletotrichum lindemuthianum in dry edible beans. Presented at: Bean Improv. Coop. Biennial Meeting; Nov. 2-4; Niagara Falls, Ontario, Canada.

Hart, J.P. and P.D. Griffiths. 2015. Genotyping-by-sequencing enabled mapping and marker development for the By-2 potyvirus resistance allele in common bean. Plant Genome 8:1-14.

Hart, J.P. and P.D. Griffiths. 2014. Resistance to Clover yellow vein virus in common bean germplasm. Crop Sci. 54: 2609-2618.

Hoyos-Villegas, V., W. Mkwaila, P.B. Cregan and J.D. Kelly. 2015. QTL analysis of white mold avoidance in pinto bean (Phaseolus vulgaris). Crop Sci. 55:2116-2129. doi:10.2135/cropsci2015.02.0106.

Lamppa, R.S., J.M. Halvorson, and J.S. Pasche. 2015. Production of anthracnose infected dry bean seed under greenhouse conditions. Presented at: Bean Improv. Coop. Biennial Meeting; Nov. 2-4; Niagara Falls, Ontario, Canada.

Kamfwa, K., K.A. Cichy, and J.D. Kelly. 2015. Genome-wide association analysis of symbiotic nitrogen fixation in common bean. Theoretical and Applied Genetics 128:1999-2017. doi. 10.1007/s00122-015-2562-5.

Kamfwa, K., K.A Cichy, and J. Kelly. 2015. Genome-wide association study of agronomic traits in common bean. The Plant Genome 8: doi:10.3835/plantgenome2014.09.0059.

Kelly, J.D., G.V. Varner, K.A. Cichy, and E.M. Wright. 2015. Registration of ‘Alpena’ Navy Bean. J. Plant Registrations 9:10-14.

Kelly, J.D., G.V. Varner, K.A. Cichy, and E.M. Wright. 2015. Registration of ‘Zenith’ Black Bean. J. Plant Registrations 9:15-20.

Kelly J.D., J. Trapp, P. Miklas, K.A. Cichy, and E.M. Wright. 2015. Registration of ‘Desert Song’ Flor de Junio and ‘Gypsy Rose’ Flor de Mayo Common Bean Cultivars. J. Plant Registrations 9:133-137.

Khankhum S., R. Valverde, M. Pastor-Corrales, J.M. Osorno, and S. Sabanadzovic. 2015. Two endornaviruses show differential infection patterns between gene pools of Phaseolus vulgaris. Arch. Virol. 160:1131-1137.

Mathew, F.M., L.A. Castlebury, K. Alananbeh, J.G. Jordahl, C.A. Taylor, S.M. Meyer, R.S. Lamppa, J.S. Pasche, and S.G. Markell. 2015. Identification of Diaporthe longicolla on dry edible pea, dry edible bean, and soybean in North Dakota. Plant Health Progress 16:71-72. doi:10.1094/PHP-RV-14-0045.

Pasche, J.S., R.S. Lamppa, J.M. Osorno, and P. Miklas. 2015. Multiple disease resistance in dry edible pinto bean breeding lines obtained by marker-assisted selection. Phytopathology 105(Suppl. 4):S4.108.

Porch, T.G., J.S. Beaver, G. Abawi, C. Estévez de Jensen and J.R. Smith. 2014. Registration of a small-red dry bean germplasm, TARS-LFR1, with multiple disease resistance and superior performance in low nitrogen soils. Journal of Plant Registrations 8:177-182.

Porch, T.G., J.S. Beaver, S. Colom, A. Vargas, Y. Trukhina and C. Estevez de Jensen. 2014. Development of tools for Macrophomina phaseolina evaluation and for genetic improvement of common bean. Ann. Rep. Bean Improv. Coop. 57:189-190.

Schröder S., S. Mamidi, R. Lee, M.R. McKain, P.E. McClean, and J.M. Osorno. 2015. Optimization of genotyping by sequencing (GBS) data in common bean (Phaseolus vulgaris L.). Mol. Breeding (Accepted).

Schröder S., S. Mafi-Moghaddam, A. Soltani, R. Lamppa, S. Mamidi, P.E. McClean, J.S. Pasche, and J.M. Osorno. 2015. Alternative screening method reveals partial anthracnose resistance to race 73 in 18 genotypes of the mesoamerican diversity panel. Presented at: Bean Improv. Coop. Biennial Meeting; Nov. 2-4; Niagara Falls, Ontario, Canada.

Singh, S.P., and P.N. Miklas. 2015. Breeding common bean for resistance to common blight: A review. Crop Sci. 55:971-984.

Soltani A., M. Bello, J.M. Osorno, P.M. Miklas, P.E. McClean. 2015. Phenotypic and molecular analysis of the transition to type II growth habit in common bean Presented at: Bean Improv. Coop. Biennial Meeting; Nov. 2-4; Niagara Falls, Ontario, Canada.

Soltani A., S. Mafi-Moghaddam, K. Walter, K. Ghising, J. Vasquez-Guzman, S. Schröder, C.F. Velasquez, E.G. Escobar, R. Lee, P. McClean, and J.M. Osorno. 2015. Developing a waterproof dry bean (Phaseolus vulgaris L.): identifying genotypes and genomic regions associated with waterlogging tolerance. Presented at: Bean Improv. Coop. Biennial Meeting; Nov. 2-4; Niagara Falls, Ontario, Canada.

Soltani A., S. Mafi-Moghaddam, J.M. Osorno, P. McClean. 2015. Identifying genomic regions controlling plant architectural characteristics in dry bean (Phaseolus vulgaris L.). Presented at: Plant and Animal Genome XXIII; Jan. 10-14; San Diego, CA.

Song Q., G. Jia, D.L. Hyten, J. Jenkins, E.Y. Hwang, S.G. Schroeder, J.M. Osorno, J. Schmutz, S.A. Jackson, P.E. McClean, and P.B. Cregan. 2015. SNP assay development for linkage map construction, anchoring whole genome sequence and other genetic and genomic applications in common bean. G3: 5:2285-2290. doi:10.1534/g3.115.020594.

Sousa, L.L., A.O. Gonçalves, M.C. Gonçalves-Vidigal, G.F. Lacanallo, A.C. Fernandez, H. Awale, and J.D. Kelly. 2015. Genetic characterization and mapping of anthracnose resistance of Corinthiano common bean landrace cultivar. Crop Sci. 55:1900-1910. doi:10.2135/cropsci2014.09.0604.

Traub, J., M. Naeem, J. Kelly, G. Austic, D. Kramer, and W. Loescher. 2015. Phenotyping for heat tolerance in bean (Phaseolus spp.) using new and conventional fluorescence and gas exchange parameters. Poster presented at: JAHS Annual Meeting; Aug. 1-4; New Orleans, LA.

Tvedt, C., S.G. Markell, and J.S. Pasche. 2015. Efficacy of in-furrow fungicides for management of Rhizoctonia root rot of dry bean. Phytopathology abstract (In Press).

Viteri, D., K. Otto, H. Terán, H. Schwartz, and S.P. Singh. 2015. Use of four Sclerotinia sclerotiorum isolates of different aggressiveness with multiple inoculations and evaluations to select common beans with high levels of white mold resistance. Euphytica 204:457-472.

Viteri, D. and S.P. Singh. 2015. Inheritance of white mold resistance in an Andean common bean A 195 and its relationship with G122. Crop Sci. 55:44-49.

Walter K., A. Soltani, C.F. Velasquez, E.G. Escobar, and J.M. Osorno. 2015. Identifying waterlogging tolerant dry bean (Phaseolus vulgaris L.) genotypes using chlorophyll content. Presented at: Bean Improv. Coop. Biennial Meeting; Nov. 2-4; Niagara Falls, Ontario, Canada.

Zuiderveen, G.H., K. Kamfwa and J.D. Kelly. 2015. Anthracnose resistance in Andean beans. Ann. Rep. Bean Improv. Coop. 58:9-10.

Zuiderveen, G.H., and J.D. Kelly. 2015. Genome-wide association study of anthracnose resistance in Andean beans. Poster presented at: JAHS Annual Meeting; Aug. 1-4; New Orleans, LA.