SAES-422 Multistate Research Activity Accomplishments Report
Sections
Status: Approved
Basic Information
- Project No. and Title: S1015 : Host Resistance as the Cornerstone for Managing Plant-Parasitic Nematodes in Sustainable Agroecosystems (S-282)
- Period Covered: 01/01/2002 to 12/31/2002
- Date of Report: 01/04/2004
- Annual Meeting Dates: 11/03/2003 to 11/04/2003
Participants
Alabama, Auburn University Kathy S. Lawrence* Arkansas, University of Arkansas Terry Kirkpatrick* Louisiana, Louisiana State University Edward C. McGawley* Minnesota, University of Minnesota Senyu Chen* Mississippi, Mississippi State University Gary W. Lawrence* North Carolina, North Carolina State University Steven L. Koenning* South Carolina, Clemson University Steve A. Lewis* Tennessee, University of Tennessee Ernie C. Bernard* Texas, Texas A&M University Jim L. Starr* USDA, ARS, Mississippi Salliana Stetina USDA, ARS, Tennessee Pat Donald Virginia, Virginia Tech Charles S. Johnson Ron Lacewell, Administrative Advisor, Texas Guests: Stan Usery, Student Auburn University Jararod Jones, Student, Auburn University Ken Stetina, Delta Research and Education Center. Mississippi State University
Present:
Ernie Bernard, Tennessee
Senyu Chen, Minnesota
Chuck Johnson, Virginia
Terry Kirkpatrick, Arkansas
Steve Koenning, North Carolina
Gary Lawrence, Mississippi
Steve Lewis, South Carolina
J. L. Starr, Texas
Salliana Stetina, USDA-ARS, Mississippi
Pat Donald, USDA-ARS, Tennessee
Ed McGawley, Louisiana
Kathy Lawrence, Alabama
Ron Lacewell, Administrative Advisor, Texas
Guests:
Stan Usery, student, Auburn University
Jararod Jones, student, Auburn University
Ken Stetina, Delta Education and Research Center, Mississippi State University
Minutes:
Dr. Gary Lawrence of Mississippi State University called the meeting to order at approximately 8:45 a.m. and welcomed all attendees to Biloxi, Mississippi and to the meeting. Before beginning state reports, those present thanked Jim Starr for his work and leadership in preparing the new S-1015 project. It was also noted that Dr. Eric Davis former student, Dr. Melissa Goellner, had been hired as nematologist at the University of Missouri.
Twenty minute-long oral reports were then presented by Steve Koenning of North Carolina State University, Chuck Johnson from Virginia Tech, Ernie Bernard from the University of Tennessee, Pat Donald from the USDA-ARS unit in Jackson, Tennessee, Terry Kirkpatrick from the University of Arkansas, Jim Starr from Texas A&M University, Steve Lewis of Clemson University, and Senyu Chen from the University of Minnesota.
State reports resumed at 8:30 a.m. on Tuesday, November 2 with reports from Kathy McLean and her students from Auburn University. Salliana Stetina presented final reports for the meeting from the USDA-ARS unit in Stoneville, Mississippi and Gary Lawrence from Mississippi State University.
A short business meeting began at 11 a.m. Dr. Ron Lacewell of Texas A&M University was introduced as the groups new administrative advisor. Ron suggested that the committee develop a brochure over the next year to document its activity and progress. The format of the brochure should include bullet points, pictures, and short impact statements. Ron also suggested that the committee maintain an interesting and informative website with links to SON, APS, and IPM sites on the Internet. Cross-state collaboration and cooperation were emphasized, as was the continuing need to update publication lists for work related to the project. Kathy McLean was complimented for including students in the meeting. Ron requested a list of email addresses for all project participants and encouraged participants to continue to collect and report yield and economic information as well as nematode data.
South Carolina was identified as the 2004 meeting site, and Jim Starr nominated Steve Lewis as the chair for the 2004 meeting. After being elected unanimously, Steve suggested that the group meet on or near the Clemson campus. Discussion ensued regarding other scientists who might be encouraged to attend the 2004 meeting.
All participants were encouraged to check with their administrators regarding official participation in the project, i.e., proper completion of form E.
Jim Starr moved, and Ernie Bernard seconded, a motion to thank Gary Lawrence for his excellent performance in hosting the 2003 meeting. Jim also suggested that the group draft a letter to the University of Kentucky thanking Fred Knapp for his service to the committee. It was also suggested that this letter be copied to Eric Young, Executive Director of the Southern Association of Experiment Station Directors.
The meeting adjourned at 11:35 a.m.
Respectfully submitted,
Chuck Johnson
Ernie Bernard, Tennessee
Senyu Chen, Minnesota
Chuck Johnson, Virginia
Terry Kirkpatrick, Arkansas
Steve Koenning, North Carolina
Gary Lawrence, Mississippi
Steve Lewis, South Carolina
J. L. Starr, Texas
Salliana Stetina, USDA-ARS, Mississippi
Pat Donald, USDA-ARS, Tennessee
Ed McGawley, Louisiana
Kathy Lawrence, Alabama
Ron Lacewell, Administrative Advisor, Texas
Guests:
Stan Usery, student, Auburn University
Jararod Jones, student, Auburn University
Ken Stetina, Delta Education and Research Center, Mississippi State University
Minutes:
Dr. Gary Lawrence of Mississippi State University called the meeting to order at approximately 8:45 a.m. and welcomed all attendees to Biloxi, Mississippi and to the meeting. Before beginning state reports, those present thanked Jim Starr for his work and leadership in preparing the new S-1015 project. It was also noted that Dr. Eric Davis former student, Dr. Melissa Goellner, had been hired as nematologist at the University of Missouri.
Twenty minute-long oral reports were then presented by Steve Koenning of North Carolina State University, Chuck Johnson from Virginia Tech, Ernie Bernard from the University of Tennessee, Pat Donald from the USDA-ARS unit in Jackson, Tennessee, Terry Kirkpatrick from the University of Arkansas, Jim Starr from Texas A&M University, Steve Lewis of Clemson University, and Senyu Chen from the University of Minnesota.
State reports resumed at 8:30 a.m. on Tuesday, November 2 with reports from Kathy McLean and her students from Auburn University. Salliana Stetina presented final reports for the meeting from the USDA-ARS unit in Stoneville, Mississippi and Gary Lawrence from Mississippi State University.
A short business meeting began at 11 a.m. Dr. Ron Lacewell of Texas A&M University was introduced as the groups new administrative advisor. Ron suggested that the committee develop a brochure over the next year to document its activity and progress. The format of the brochure should include bullet points, pictures, and short impact statements. Ron also suggested that the committee maintain an interesting and informative website with links to SON, APS, and IPM sites on the Internet. Cross-state collaboration and cooperation were emphasized, as was the continuing need to update publication lists for work related to the project. Kathy McLean was complimented for including students in the meeting. Ron requested a list of email addresses for all project participants and encouraged participants to continue to collect and report yield and economic information as well as nematode data.
South Carolina was identified as the 2004 meeting site, and Jim Starr nominated Steve Lewis as the chair for the 2004 meeting. After being elected unanimously, Steve suggested that the group meet on or near the Clemson campus. Discussion ensued regarding other scientists who might be encouraged to attend the 2004 meeting.
All participants were encouraged to check with their administrators regarding official participation in the project, i.e., proper completion of form E.
Jim Starr moved, and Ernie Bernard seconded, a motion to thank Gary Lawrence for his excellent performance in hosting the 2003 meeting. Jim also suggested that the group draft a letter to the University of Kentucky thanking Fred Knapp for his service to the committee. It was also suggested that this letter be copied to Eric Young, Executive Director of the Southern Association of Experiment Station Directors.
The meeting adjourned at 11:35 a.m.
Respectfully submitted,
Chuck Johnson
Accomplishments
OBJECTIVE 1: Identification, characterization and introgression of genes for resistance and tolerance to nematodes into cotton, peanut, soybean and major fruit and vegetable crops.
Cotton:
AL - In Alabama, cotton varieties were examined in the greenhouse and the field to determine possible resistance and/or tolerance to the reniform nematode, Rotylenchulus reniformis. Reniform nematode numbers in the greenhouse increased in all of the varieties. Reproductive factors (Rf) varied from a low of 13 in Sure-Grow 747 to a high in Phytogen GA 161 of 39. In the annual corn cotton rotation field, reniform numbers increased on all varieties from the initial population of 50 vermiform per 150 cc of soil at planting. The phenotypic correlation between Rf and cottonseed yield was non-significant. In a monoculture cotton production system the reniform numbers increased in 83% of the varieties with Rf of greater than 1. Correlations between yield and nematicide application were not significant.
Greenhouse evaluation of the TX USDA germplasm collection for resistance to the reniform nematode suggests a consistent trend between the numbers of vermiform and egg recovered at 60 days after planting. Selecting those genotypes with the lowest R. reniformis numbers and fewest eggs produced will undergo a second screening.
AR - Stoneville LA, Stoneville 5599 BR and Paymaster 1560 were compared in greenhouse and field evaluations for their reaction to the root-knot nematode.
Stoneville LA 887 and Paymaster 1560 BG/RR both exhibited moderate resistance.
In the greenhouse, Stoneville LA 887, Stoneville 5599BR and Paymaster 1560 were similar in galling severity.
LA Cotton cultivars Deltapine NuCotton 33B, Deltapine DP 436RR, FiberMax FM 832, Stoneville ST 474, and SureGrow SG 747 were used in greenhouse studies to determine host suitability and susceptibility to reniform nematod, and supported nematode reproduction with levels as much as 100-fold.
Nine isolates of the reniform nematode, Rotylenchulus reniformis, collected from Arkansas, Georgia, Hawaii (2 isolates), Louisiana (2 isolates), Mississippi and Texas propagated from single egg masses in the lab were used in greenhouse host range studies. Over two trials, R-values among the 9 reniform nematode populations differed significantly, especially on tomato, cotton, and pepper.
MS - Seven cotton lines were examined with the reniform nematode with all susceptible to the reniform with reproductive indexes ranging from 34.1 to 109.51. Fourteen lines were examined for resistance to the root-knot nematode, thirteen, had a reproductive index less than one and would be considered resistant to root-knot nematode.
Four conventional cotton varieties and seventeen transgenic cotton varieties were were susceptible with reproduction factors ranging from 9.9 to 139.
TX - Cotton - In a BC2 generation population with Gossypium longicalyx as the resistant non-recurrent parent, 36 of 56 plants tested supported reproduction of reniform nematodes that was <5% of that on the susceptible DeltaPine 16. In a BC3 generation population, 35 of 200 plants were resistant with <5% of the reproduction of reniform nematodes as on susceptible DeltaPine 16. To introgress resistance to R. reniformis from G. barbadense, we have developed of a BC1F1 population from Tx110 x M315 with M315 as the recurrent parent. Evaluation of four F4:5 families derived from the Tx110 x M315 cross for resistance to R. reniformis indicates that resistance is a fixed trait in each.
Peanut:
TX - Three backcross generations have been completed in efforts to introgress nematode resistance in to peanut genotypes with resistance to TSWV and into genotypes with the High O/L trait.
Soybean:
MN - Four-year experiments were initiated in 2002 at three field sites in Minnesota to evaluate the cover crops alfalfa, red clover, and perennial ryegrass inter-seeded with soybean. Preliminary data indicated that red clover and alfalfa reduced SCN population density as compared with no-cover-crop control. In biological control studies, Hirsutella rhossiliensis and Hirsutella minnesotensis were effective in control of the soybean cyst nematode. In 2003, a greenhouse study determined whether the fungi could establish their population with extremely low inoculation levels. Hirsutella minnesotensis established its population over time in the natural soil even at very low inoculation levels. H. rhossiliensis had little activity 2, 5, 7 months after adding the fungus at low inoculation levels.
MS - Twenty-two soybean germplasm entries were were considered susceptible to the reniform nematode.
NC - The identification and functional analyses of parasitism genes from soybean cyst and root-knot nematodes showed parasitism genes encode products secreted by the nematodes that enable successful infection of host crop roots. A number of the nematode parasitism genes appear to mimic the activity of (plant) host genes and may directly affect the interaction of the nematode with the plant. Three advanced lines with SCN resistance superior to cv. Fowler are currently being yield tested as cultivars. F2 populations with high levels of resistance to SCN and the herbicide roundup are being advance, and new crosses with Delsoy 5710 and herbicide resistant cultivars made. Soybean lines derived from cvs. Anand, Fowler, and Cordell were screened for SCN resistance. Both cvs. Anand and Fowler are resistant to race 1 of SCN.
Approximately fifty genes encoding esophageal gland proteins secreted by the soybean cyst nematode (SCN), Heterodera glycines were characterized. Products of the SCN secretory genes may serve as targets for neutralization to develop novel and durable resistance in transgenic soybean plants. The expression of different secretory genes in SCN was determined to be developmentally-regulated, suggesting specific genes to target to develop bioengineered resistance.
SC - Soybean genotypes from the soybean-breeding program are being screened (over 850 lines) in the greenhouse against races 3 and 14 of the soybean cyst nematode. Advanced lines are developed with Roundup Ready genetics.
Nine commercially available RR. cultivars and six SC RR. experimental lines (Maturity Groups VI VIII) were planted in fields infested with SCN race 14, M. incognita, M. arenaria, and Columbia lance nematode. Root gall and plant vigor ratings were taken in the root-knot fields.
USDA/ARS- MS - Breeding results from 2003 have resulted in F2: 3 progeny from crosses made among day-neutral selections of Gossypium hirsutum (T19-12, T19-13, T19-27, T19-30) and G. barbadense (T1347-2, T1347-31). Of the 889 F2: 3 families evaluated, 117 were selected for further assessment and possible advancement to stabilize the resistant phenotype. Of the 150 race stock populations evaluated, 53 were selected for further assessment and possible advancement to stabilize the resistant phenotype.
USDA/ARS - TN - Ten SCN populations maintained for screening of soybean germplasm is being characterized over time using the standard race differentials and the eight HG type indicator lines. Reproduction is measured on soybean cultivars containing PI 437654 as the source of resistance. We have a low reproduction on Anand and a CystX line by two of our field populations and high reproduction on these cultivars by LY1 And LY2 populations.
OBJECTIVE 2: Development of marker-assisted selection systems for more efficient introgression of multiple resistance genes into agronomically superior crop genotypes.
Novel Technique:
SC - A PCR technique is being adapted for supplemental use to identify the four major species of Meloidogyne. Electrophoresis of esterases and perineal pattern analysis of adult females are the primary, proven techniques. Now we have primers for each species that will cut DNA into 420 (Mj), 700 (Mh), 1050 (Ma), and 1350 (Mi) base pair products, with the primer pairs being designed to give easily visible separation on the gel.
Cotton:
TX - Cotton - Two loci with modest linkage to loci for resistance to M. incognita have been identified (A1214 on LGA02 at 18.6 cM from a resistance locus and pAR815 on Chromosome 14 at 21.6 cM from a resistance locus).
Peanut:
NC - Over three hundred peanut plants - F2 progeny of crosses between peanut cultivar COAN (from Texas A & M) and breeding lines GP NC WS-5 and GP NC WS-6, and appropriate controls) were analyzed by traditional screening and through the use of RAPD markers for resistance to Meloidogyne arenaria. The data indicates that resistance in COAN and North Carolina Breeding lines are controlled by the same dominant gene (Tallury, Stalker, Koenning).
OBJECTIVE 3: Deployment of resistance and tolerance to nematodes in sustainable cropping systems.
Cotton:
AL - Thirty-two winter cover crops were evaluated as potential host of the reniform nematode. Crimson clover and vetch were compared to wheat, rye, oats, ryegrass, subterranean clover, lupines and fallow treatments in the field over the winter months. Reniform numbers did not increase on any of the cover crops when sampled monthly from November through April, however they did increase through out the summer month on cotton.
AR - Forty-four soils were sample assayed for soybean cyst nematode race analysis. Races 2 and 6 were the most frequently identified. Race 3 was not found, and races 9 and 14 were found in only 9 of the fields
LA - Two experimental pesticides/nematicides Agri-Terra and Agrizide were compared for management of indigenous nematode and weed species, where yield was increased significantly by 10.8 kilograms by Agri Terra.
Agrizide was applied to soil, which was infested with indigenous soilborne nematodes, weeds, and other pathogens. Neniform nematode population density was reduced by 90 to 95 percent. On soybean and sugarcane, agrizide treatments reduced nematode populations. With tomato and pepper, both root knot and reniform nematode levels in Agrizide treated microplots were below detectable levels.
NC - An experiment where a field is infested with the southern root-knot nematode Meloidogyne incognita was done. This experiment is designed to have root-knot resistant and susceptible cultivars and run through 2009 (Koenning, and Edmisten).
Experiments with treated municipal sewer sludge for management of root-knot and soybean cyst nematode were established in 2002. A third project that focuses on the value of resistance to SCN was continued in Washington and Lenoir Cos. NC.
Soybean:
LA - On sugarcane treatments include Agri-Terra application Agrizide, Methyl Bromide, and a non-treated control. Both rates of Agrizide reduced nematode and weed populations; superior to Methyl Bromide. There was some early evidence of agrizide-related phytotoxicity to sugarcane. However, direct examination of planted stalks showed no evidence of damage.
USDA/ARS - TN - Long-term field plots are established in 1979 to examine the effects of tillage regimes on soil characteristics and soybean yield and are currently in progress. In 2002 soybean cyst nematode population densities across treatments was similar in the upper eight inches of soil in all plots.
Selected Crops:
VA - Three on farm tests and three small plot tests were conducted in 2003 to
evaluate cultivars and breeding lines for resistance to Globodera tabacum solanacearum. Nematode resistant cultivars suppressed nematode population development across nematicide treatments, although statistically significant increases in yield didn‘t always result. Hybrid breeding lines developed for resistance to Meloidogyne incognita, M. arenaria, and M. javanica appeared to also possess resistance to G. t. solanacearum.
Cotton:
AL - In Alabama, cotton varieties were examined in the greenhouse and the field to determine possible resistance and/or tolerance to the reniform nematode, Rotylenchulus reniformis. Reniform nematode numbers in the greenhouse increased in all of the varieties. Reproductive factors (Rf) varied from a low of 13 in Sure-Grow 747 to a high in Phytogen GA 161 of 39. In the annual corn cotton rotation field, reniform numbers increased on all varieties from the initial population of 50 vermiform per 150 cc of soil at planting. The phenotypic correlation between Rf and cottonseed yield was non-significant. In a monoculture cotton production system the reniform numbers increased in 83% of the varieties with Rf of greater than 1. Correlations between yield and nematicide application were not significant.
Greenhouse evaluation of the TX USDA germplasm collection for resistance to the reniform nematode suggests a consistent trend between the numbers of vermiform and egg recovered at 60 days after planting. Selecting those genotypes with the lowest R. reniformis numbers and fewest eggs produced will undergo a second screening.
AR - Stoneville LA, Stoneville 5599 BR and Paymaster 1560 were compared in greenhouse and field evaluations for their reaction to the root-knot nematode.
Stoneville LA 887 and Paymaster 1560 BG/RR both exhibited moderate resistance.
In the greenhouse, Stoneville LA 887, Stoneville 5599BR and Paymaster 1560 were similar in galling severity.
LA Cotton cultivars Deltapine NuCotton 33B, Deltapine DP 436RR, FiberMax FM 832, Stoneville ST 474, and SureGrow SG 747 were used in greenhouse studies to determine host suitability and susceptibility to reniform nematod, and supported nematode reproduction with levels as much as 100-fold.
Nine isolates of the reniform nematode, Rotylenchulus reniformis, collected from Arkansas, Georgia, Hawaii (2 isolates), Louisiana (2 isolates), Mississippi and Texas propagated from single egg masses in the lab were used in greenhouse host range studies. Over two trials, R-values among the 9 reniform nematode populations differed significantly, especially on tomato, cotton, and pepper.
MS - Seven cotton lines were examined with the reniform nematode with all susceptible to the reniform with reproductive indexes ranging from 34.1 to 109.51. Fourteen lines were examined for resistance to the root-knot nematode, thirteen, had a reproductive index less than one and would be considered resistant to root-knot nematode.
Four conventional cotton varieties and seventeen transgenic cotton varieties were were susceptible with reproduction factors ranging from 9.9 to 139.
TX - Cotton - In a BC2 generation population with Gossypium longicalyx as the resistant non-recurrent parent, 36 of 56 plants tested supported reproduction of reniform nematodes that was <5% of that on the susceptible DeltaPine 16. In a BC3 generation population, 35 of 200 plants were resistant with <5% of the reproduction of reniform nematodes as on susceptible DeltaPine 16. To introgress resistance to R. reniformis from G. barbadense, we have developed of a BC1F1 population from Tx110 x M315 with M315 as the recurrent parent. Evaluation of four F4:5 families derived from the Tx110 x M315 cross for resistance to R. reniformis indicates that resistance is a fixed trait in each.
Peanut:
TX - Three backcross generations have been completed in efforts to introgress nematode resistance in to peanut genotypes with resistance to TSWV and into genotypes with the High O/L trait.
Soybean:
MN - Four-year experiments were initiated in 2002 at three field sites in Minnesota to evaluate the cover crops alfalfa, red clover, and perennial ryegrass inter-seeded with soybean. Preliminary data indicated that red clover and alfalfa reduced SCN population density as compared with no-cover-crop control. In biological control studies, Hirsutella rhossiliensis and Hirsutella minnesotensis were effective in control of the soybean cyst nematode. In 2003, a greenhouse study determined whether the fungi could establish their population with extremely low inoculation levels. Hirsutella minnesotensis established its population over time in the natural soil even at very low inoculation levels. H. rhossiliensis had little activity 2, 5, 7 months after adding the fungus at low inoculation levels.
MS - Twenty-two soybean germplasm entries were were considered susceptible to the reniform nematode.
NC - The identification and functional analyses of parasitism genes from soybean cyst and root-knot nematodes showed parasitism genes encode products secreted by the nematodes that enable successful infection of host crop roots. A number of the nematode parasitism genes appear to mimic the activity of (plant) host genes and may directly affect the interaction of the nematode with the plant. Three advanced lines with SCN resistance superior to cv. Fowler are currently being yield tested as cultivars. F2 populations with high levels of resistance to SCN and the herbicide roundup are being advance, and new crosses with Delsoy 5710 and herbicide resistant cultivars made. Soybean lines derived from cvs. Anand, Fowler, and Cordell were screened for SCN resistance. Both cvs. Anand and Fowler are resistant to race 1 of SCN.
Approximately fifty genes encoding esophageal gland proteins secreted by the soybean cyst nematode (SCN), Heterodera glycines were characterized. Products of the SCN secretory genes may serve as targets for neutralization to develop novel and durable resistance in transgenic soybean plants. The expression of different secretory genes in SCN was determined to be developmentally-regulated, suggesting specific genes to target to develop bioengineered resistance.
SC - Soybean genotypes from the soybean-breeding program are being screened (over 850 lines) in the greenhouse against races 3 and 14 of the soybean cyst nematode. Advanced lines are developed with Roundup Ready genetics.
Nine commercially available RR. cultivars and six SC RR. experimental lines (Maturity Groups VI VIII) were planted in fields infested with SCN race 14, M. incognita, M. arenaria, and Columbia lance nematode. Root gall and plant vigor ratings were taken in the root-knot fields.
USDA/ARS- MS - Breeding results from 2003 have resulted in F2: 3 progeny from crosses made among day-neutral selections of Gossypium hirsutum (T19-12, T19-13, T19-27, T19-30) and G. barbadense (T1347-2, T1347-31). Of the 889 F2: 3 families evaluated, 117 were selected for further assessment and possible advancement to stabilize the resistant phenotype. Of the 150 race stock populations evaluated, 53 were selected for further assessment and possible advancement to stabilize the resistant phenotype.
USDA/ARS - TN - Ten SCN populations maintained for screening of soybean germplasm is being characterized over time using the standard race differentials and the eight HG type indicator lines. Reproduction is measured on soybean cultivars containing PI 437654 as the source of resistance. We have a low reproduction on Anand and a CystX line by two of our field populations and high reproduction on these cultivars by LY1 And LY2 populations.
OBJECTIVE 2: Development of marker-assisted selection systems for more efficient introgression of multiple resistance genes into agronomically superior crop genotypes.
Novel Technique:
SC - A PCR technique is being adapted for supplemental use to identify the four major species of Meloidogyne. Electrophoresis of esterases and perineal pattern analysis of adult females are the primary, proven techniques. Now we have primers for each species that will cut DNA into 420 (Mj), 700 (Mh), 1050 (Ma), and 1350 (Mi) base pair products, with the primer pairs being designed to give easily visible separation on the gel.
Cotton:
TX - Cotton - Two loci with modest linkage to loci for resistance to M. incognita have been identified (A1214 on LGA02 at 18.6 cM from a resistance locus and pAR815 on Chromosome 14 at 21.6 cM from a resistance locus).
Peanut:
NC - Over three hundred peanut plants - F2 progeny of crosses between peanut cultivar COAN (from Texas A & M) and breeding lines GP NC WS-5 and GP NC WS-6, and appropriate controls) were analyzed by traditional screening and through the use of RAPD markers for resistance to Meloidogyne arenaria. The data indicates that resistance in COAN and North Carolina Breeding lines are controlled by the same dominant gene (Tallury, Stalker, Koenning).
OBJECTIVE 3: Deployment of resistance and tolerance to nematodes in sustainable cropping systems.
Cotton:
AL - Thirty-two winter cover crops were evaluated as potential host of the reniform nematode. Crimson clover and vetch were compared to wheat, rye, oats, ryegrass, subterranean clover, lupines and fallow treatments in the field over the winter months. Reniform numbers did not increase on any of the cover crops when sampled monthly from November through April, however they did increase through out the summer month on cotton.
AR - Forty-four soils were sample assayed for soybean cyst nematode race analysis. Races 2 and 6 were the most frequently identified. Race 3 was not found, and races 9 and 14 were found in only 9 of the fields
LA - Two experimental pesticides/nematicides Agri-Terra and Agrizide were compared for management of indigenous nematode and weed species, where yield was increased significantly by 10.8 kilograms by Agri Terra.
Agrizide was applied to soil, which was infested with indigenous soilborne nematodes, weeds, and other pathogens. Neniform nematode population density was reduced by 90 to 95 percent. On soybean and sugarcane, agrizide treatments reduced nematode populations. With tomato and pepper, both root knot and reniform nematode levels in Agrizide treated microplots were below detectable levels.
NC - An experiment where a field is infested with the southern root-knot nematode Meloidogyne incognita was done. This experiment is designed to have root-knot resistant and susceptible cultivars and run through 2009 (Koenning, and Edmisten).
Experiments with treated municipal sewer sludge for management of root-knot and soybean cyst nematode were established in 2002. A third project that focuses on the value of resistance to SCN was continued in Washington and Lenoir Cos. NC.
Soybean:
LA - On sugarcane treatments include Agri-Terra application Agrizide, Methyl Bromide, and a non-treated control. Both rates of Agrizide reduced nematode and weed populations; superior to Methyl Bromide. There was some early evidence of agrizide-related phytotoxicity to sugarcane. However, direct examination of planted stalks showed no evidence of damage.
USDA/ARS - TN - Long-term field plots are established in 1979 to examine the effects of tillage regimes on soil characteristics and soybean yield and are currently in progress. In 2002 soybean cyst nematode population densities across treatments was similar in the upper eight inches of soil in all plots.
Selected Crops:
VA - Three on farm tests and three small plot tests were conducted in 2003 to
evaluate cultivars and breeding lines for resistance to Globodera tabacum solanacearum. Nematode resistant cultivars suppressed nematode population development across nematicide treatments, although statistically significant increases in yield didn‘t always result. Hybrid breeding lines developed for resistance to Meloidogyne incognita, M. arenaria, and M. javanica appeared to also possess resistance to G. t. solanacearum.
Impacts
- Agrizide (an experimental nematicide) reduced nemotode population density in cotton, tomato, pepper, and sugarcane and was superior to methyl bromide on sugarcane.
- Cotton lines with resistance to to reproduction of reniform nemotodes are evolving including both conventional and transgenic varieties.
- Marker-assisted selection of genes for resistence indicates that resistence in peanut plants, COAN and North Carolina Breeding lines are controlled by the same dominant gene.
Publications
AL - Publications:
Diez, Asdrubal, G. W. Lawrence and K. S. McLean. 2003. Nematode post-infection development on cotton following separate and concomitant parasitism by Meloidogyne incognita and Rotylenchulus reniformis. Journal of Nematology (In Press).
McLean, K. S. and G. W. Lawrence. 2003. Efficacy of Aldicarb to Rotylenchulus reniformis and biodegradation in cotton field soils. Journal of Nematology 35 (1):65-72.
Lawrence, G. W. and K. S. McLean. 2003. Foliar applications of oxamyl with aldicarb for the management of Meloidogyne incognita on cotton. Nematropica Vol 32, No. 2:103-112.
McLean, K. S. and C. Dale Monks. Eds. 2002 Cotton Research Report. March 2003. Alabama Agricultural Experiment Station Research Report Series No. 24. Pp 52.
McLean, K. S. G. W. Lawrence, C. Overstreet, and L. D. Young. 2003. Efficacy of anhydrous ammonia on the reniform nematode in cotton. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:282-283 (2003) National Cotton Council, Memphis TN.
Usery, S. R., K. S. McLean, C. Burmester, E. Van Saten, and Brad Meyer. 2003. Response of selected cotton varieties to the reniform nematode in Alabama. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:261-264 (2003) National Cotton Council, Memphis TN.
Hutchinson, J. L., J. R. Jones, K. S. McLean, and J. Williams. 2003. Evaluation of selected cover crops to determine host suitability for Rotylenchulus reniformis. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:248-249 (2003) National Cotton Council, Memphis TN.
Palmateer, A. J., K. S. McLean, and E. Van Saten. 2003. Interaction of Rotylenchulus reniformis with seedling disease pathogens of cotton. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:222-225 (2003) National Cotton Council, Memphis TN.
Tagert, M. D., G. W. Lawrence, W. E. Batson, H. K. Lee, A. T. Kelly, and K. S. McLean. 2003. Severity of disease caused by Thielaviopsis basicola and Rotylenchulus reniformis in concomitant or sequential interactions. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:147-148 ( 2003) National Cotton Council, Memphis TN.
Tagert, M. D., G. W. Lawrence, W. E. Batson, H. K. Lee, A. T. Kelly, and K. S. McLean. 2003. Thielaviopsis basicola and Rotylenchulus reniformis as independent or interactive pathogens on cotton in different soil types. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:145-146 ( 2003) National Cotton Council, Memphis TN.
Usery, S. R., K. S. McLean, C. Burmester, E. Van Saten, and Brad Meyer. 2003. Response of selected cotton varieties to the reniform nematode in Alabama. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:261-264 (2003) National Cotton Council, Memphis TN.
Hutchinson, J. L., J. R. Jones, K. S. McLean, and J. Williams. 2003. Evaluation of selected cover crops to determine host suitability for Rotylenchulus reniformis. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:248-249 (2003) National Cotton Council, Memphis TN.
Palmateer, A. J., K. S. McLean, and E. Van Saten. 2003. Interaction of Rotylenchulus reniformis with seedling disease pathogens of cotton. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:222-225 (2003) National Cotton Council, Memphis TN.
Tagert, M. D., G. W. Lawrence, W. E. Batson, H. K. Lee, A. T. Kelly, and K. S. McLean. 2003. Severity of disease caused by Thielaviopsis basicola and Rotylenchulus reniformis in concomitant or sequential interactions. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:147-148 ( 2003) National Cotton Council, Memphis TN.
Tagert, M. D., G. W. Lawrence, W. E. Batson, H. K. Lee, A. T. Kelly, and K. S. McLean. 2003. Thielaviopsis basicola and Rotylenchulus reniformis as independent or interactive pathogens on cotton in different soil types. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:145-146 ( 2003) National Cotton Council, Memphis TN.
Hutchinson, J. L., K. S. McLean, Y. Feng, C. Burmester, and G. W. Lawrence. 2003. Accelerated breakdown of aldicarb in Alabama cotton field soil. Phytopathology 93:S38 Publication no. P-2003-0274-AMA.
Lawrence, G. W., A. T. Kelley, H. K. Lee, W. A. Giverns, and K. S. McLean. 2003. Site specific management of the reniform nematode on cotton. Phytopathology 93:S48 Publication no. P-2003-0353-AMA.
Palmateer, A.J., K.S. McLean, J. D. Mayfield, and G. Morgan-Jones. 2003. Characterization of a Phomopsis species occurring on cotton with comparison to species colonizing soybean. Phytopathology 93:S69 Publication no. P-2003-0500-AMA.
Lawrence, G. W., A. T. Kelley, H. K. Lee, K. S. McLean. 2003. Evaluation of Adage 5FS for control of Rotylenchulus reniformis on cotton in the Mississippi delta, 2000. Fungicide and Nematicide Tests (online.) Report 58:N004. DOI: 10.1094/FN58. The American Phytopathological Society, St. Paul, MN.
Lawrence, G. W., A. T. Kelley, H. K. Lee, K. S. McLean. 2003. Evaluation of Telone II for control of Rotylenchulus reniformis on cotton in the Mississippi delta, 2000. Fungicide and Nematicide Tests (online.) Report 58:N005. DOI: 10.1094/FN58. The American Phytopathological Society, St. Paul, MN.
Lawrence, G. W., A. T. Kelley, H. K. Lee, K. S. McLean. 2003. Evaluation of Nemacur 240CS for control of Rotylenchulus reniformis on cotton in Mississippi, 2000. Fungicide and Nematicide Tests (online.) Report 58:N006. DOI: 10.1094/FN58. The American Phytopathological Society, St. Paul, MN.
Lawrence, G. W., A. T. Kelley, H. K. Lee, K. S. McLean. 2003. Evaluation of Telone II for control of Meloidogyne incognita in ultra narrow row cotton in Mississippi, 2000. Fungicide and Nematicide Tests (online.) Report 58:N007. DOI: 10.1094/FN58. The American Phytopathological Society, St. Paul, MN.
Lawrence, G. W., A. T. Kelley, H. K. Lee, K. S. McLean. 2003. Evaluation of Temik 15G for control of Rotylenchulus reniformis in the Mississippi delta, 2000. Fungicide and Nematicide Tests (online.) Report 58:N008. DOI: 10.1094/FN58. The American Phytopathological Society, St. Paul, MN.
McLean, K. S., A. J. Palmateer, J. L. Hutchinson, J. Jones, G. W. Lawrence. 2003. Evaluation of Messenger with Temik 15G for control of Rotylenchulus reniformis on cotton in central Alabama, 2002. Fungicide and Nematicide Tests (online.) Report 58:N009. DOI: 10.1094/FN58. The American Phytopathological Society, St. Paul, MN.
Usery, S. R. Jr., K. S. McLean, C. B. Burmester, B. A. Meyer, E. van Santen. 2003. Transgenic cotton variety response to Rotylenchulus reniformis in a corn cotton rotation in North Alabama, 2002. Biological and Cultural Tests for Control of Plant Diseases (online.) Report No. 18:F008. DOI: 10.1094/BC18. The American Phytopathological Society, St. Paul, MN.
Usery, S. R. Jr., K. S. McLean, C. B. Burmester, B. A. Meyer, E. van Santen. 2003. Transgenic cotton variety response to Rotylenchulus reniformis in North Alabama, 2002. Biological and Cultural Tests for Control of Plant Diseases (online.) Report No. 18:F009. DOI: 10.1094/BC18. The American Phytopathological Society, St. Paul, MN.
LA Publications
C. Overstreet, E.C. McGawley and B. Padgett. 2002. Current management strategies employed against the reniform nematode (Rotylenchulus reniformis) in cotton production in Louisiana, U.S.A Nematology 4: 306-307.
Erwin, T.L., C. Overstreet, and E.C. McGawley. 2002. Nematicides against reniform nematode in Louisiana. Proceedings of the Beltwide Cotton Conferences, CD Rom, National Cotton Council, P.O. Box 820285, Memphis, TN.
Overstreet C. and E.C. McGawley. 2002-Workshop-Nematode Management: reniform/root-knot competition and reproduction. Proceedings of the Beltwide Cotton Conferences, CD Rom, National Cotton Council, P.O. Box 820285, Mamphis, TN.
Lewis, S. A., D. J. Chitwood, and E. C. McGawley. 2003. Nematode Biology, Morphology and Physiology in Dekker Encyclopedia of Plant and Crop Science, Robert M. Goodman, ed. Marcel Dekker, NY (in press).
Carter-Wientjes, C.H., J.S. Russin, D.J. Boethel, J.L. Griffin and E.C. McGawley. 2003. Feeding and Maturation by Soybean Looper (Lepidoptera: Noctuidae) Larvae on Soybean Affected by Weed, Fungus, and Nematode Pests. Journal of Economic Entomology (in press).
Bond, J.P., E.C. McGawley and J.W. Hoy. 200?. Interaction of nematodes and Pythium spp. on Sugarcane in Louisiana. Phytopathology (submitted).
MN -Publications:
Chen, S. Y., and F. J. Chen. 2003. Fungal parasitism of Heterodera glycines eggs as influenced by egg age and pre-colonization of cysts by other fungi. Journal of Nematology 35(3):
Liu, X. Z., and S. Y. Chen. 2003. Nutritional requirements of the ARF18 and Pochonia chlamydospora, fungal parasites of nematode eggs. Journal of Invertebrate Pathology 83:10-15.
Chen, S. Y., J. H. Orf, D. R. Miller, and P. J. Schaus. 2003. Performance of soybean varieties in SCN-infested and non-infested fields in Minnesota during 2002. http://sroc.coafes.umn.edu/Nematology/PDF%20Files/Variety%202002-1.pdf
Chen, S. Y., and D. W. Dickson. 2003. Biological control of nematodes by fungal antagonists. Pp. 343-403 in Z. X. Chen, S. Y. Chen, and D. W. Dickson, eds. Nematology: Advances and perspectives. Vol. 2. Nematode management and utilization. Beijing: Tsinghua University Press and CAB International. (in press)
Chen, Z. X., S. Y. Chen, and D. W. Dickson (eds.). 2003. Nematology, advances and perspectives. Vol. 1. Nematode morphology, physiology and ecology. Beijing: Tsinghua University Press and CAB International.
Chen, S. 2003. Biocontrol potential of the fungi Hirsutella rhossiliensis and H. minnesotensis against Heterodera glycines. Phytopathology 93:S123.
MS - Publications:
Lawrence, G.W., A.T. Kelley, R. L. King, J. Vickery, H.K. Lee, and K. S. McLean. 2004. Remote sensing and precision nematicide applications for Rotylenchulus reniformis management in Mississippi cotton. Nematology Monographs and Perspectives 2: (accepted as a manuscript to be published as part of an invited symposium. All manuscripts are awaiting the final approval from the editorial board).
Lawrence, G.W., S. Samson, H.K. Lee, A.T. Kelley, and W.A. Givens. 2003. Reniform nematode management with variable rate nematicide applications. Proceedings of the National Beltwide Cotton Conference, Vol. 1:273-274 (2003) National Cotton Council, Memphis TN.
H.K. Lee, G.W. Lawrence, J.L. DuBien, and A.T. Kelley. 2003. Seasonal variation in the spatial distribution of Rotylenchulus reniformis in Mississippi cotton. Proceedings of the National Beltwide Cotton Conference, Vol. 1: 245-246 (2003) National Cotton Council, Memphis
Kelley, A.T., G.W. Lawrence, R. L. King, and J. Vickery. 2003. Spectral classification of reniform nematode (Rotylenchulus reniformis) infected cotton plants. International Symposium on Remote Sensing of Environment. (Accepted for publication).
Kelley, A.T., G.W. Lawrence, J. Vickery, R. King, and H.K. Lee. 2003. Detection and population estimation of the reniform nematode on cotton using hyperspectral remotely sensed data. Proceedings of the National Beltwide Cotton Conference Vol. 1:241-244 (2003) National Cotton Council, Memphis TN.
Kelley, A.T., G.W. Lawrence, R.L. King, J. Vickery, and H.K. Lee. 2003. Spectral Classification of Reniform Nematode (Rotylenchulus reniformis) Infested Cotton Plants Using Self-Organized Maps. Journal of Nematology 32: (abstract).
Lee, H.K., G.W. Lawrence, J.L. DuBien, and A.T. Kelley. 2003. Cultural Practices and the Dispersion of the Reniform Nematode In Mississippi. Journal of Nematology 32: (abstract).
H.K. Lee, G.W. Lawrence, and A.T. Kelley. 2003. Seasonal variation in the spatial distribution of Rotylenchulus reniformis in Mississippi cotton. Phytopathology 93: S49 Publication P-2003-0358-AMA.
Vizzier-Thaxton, Y., G.W. Lawrence and C.L. Balzli. 2002. Cotton nematode control by broiler litter. Poultry Science (Suppl.): 81:55.
NC Publications:
Cervantes-Flores, J.C., G.C. Yencho, and E.L. Davis. 2002. Host reactions of sweetpotato genotypes to root-knot nematodes and variation in virulence of Meloidogyne incognita populations. HortScience 37(7): 1112-1116.
Davis, R. F., S. R. Koenning, R. C. Kemerait, T.D. Cummings, and W. D. Shurley. 2003. Rotylenchulus reniformis management in cotton with crop rotation. Journal of Nematology 35:58-64.
Gao, B., R. Allen, T. Maier, E. L. Davis, T. J. Baum, and R. S. Hussey. 2003. The parasitome of the phytonematode Heterodera glycines. Molecular Plant-Microbe Interactions 16:270-276.
Huang, G., B. Gao, T. Maier, R. Allen, E.L. Davis, T.J. Baum, and R.S. Hussey. 2003. A profile of putative parasitism genes expressed in the esophageal gland cells of the root-knot nematode, Meloidogyne incognita. Molecular Plant-Microbe Interactions 16: 376-38
Koenning, S. R., K. L. Edmisten, K. R. Barker, and D. E. Morrison. 2003. Impact of cotton production system on Hoplolaimus columbus. Journal of Nematology 35:73-77.
Koenning, S. R., K. R. Barker, K. L. Edmisten, D. T. Bowman, and D. E. Morrison. 2003. Effects of rate and time of application of poultry litter on Hoplolaimus columbus on cotton. Plant Disease 87:1244-1249.
Wrather, J. A., S. R. Koenning, and T. Anderson. 2003. Effect of diseases on soybean yields in the United States and Ontario 1999-2002. Online. Plant Health Progress doi: 10.1094/PHP-2003-0325-01-RV. http://www.plantmanagementnetwork.org/pub/php/review/2003/soybean/.
Hussey, R.S., E.L. Davis, and T.J. Baum. 2002. Secrets in secretions: genes that control nematode parasitism of plants. Brazilian Journal of Plant Physiology 14:183-194.
Baum, T.J., Hussey, R.S., and Davis, E.L. 2003. Secrets in Secretions: Analysis of the Parasitome of Heterodera glycines. IX Meeting of the International Society for Molecular Plant-Microbe Interactions, St. Petersburg, Russia.
Sukno, S., O. Shoseyov, O. Shimerling, and E. Davis. 2003. Expression of truncated Arabidopsis thaliana Cel1 promoter during compatible plant-nematode interactions. Phytopathology 93:S81.
Huang, G., R. Dong, T. Maier, R. Allen, E. Davis, T. Baum, and R. Hussey. 2003. An efficient subtractive strategy for the identification of parasitism genes of Meloidogyne incognita. Phytopathology 93:S37.
Wang, X., Allen, R., Maier, T., Hussey, R.S., Baum, T.J., and Davis, E.L. 2003. Functional analysis of a parasitism gene from Heterodera glycines with similarity to CLAVATA3 of Arabidopsis thaliana. Phytopathology 93:S90.
Diab, H.G., B. Gao, T. J. Baum, R. S. Hussey, and E. L. Davis. 2003. Immunodetection of parasitism proteins in Heterodera glycines. Phytopathology 93:S21.
Wong, M. -Y., E. L. Davis, and J. -S. Huang. 2003. Nitric oxide synthase in higher plants: An attempt to clone the gene. Phytopathology 93:S91.
Elling, A.A., B. Gao, R. S. Hussey, E. L. Davis, and T. J. Baum. 2003. Plant nuclear import of Heterodera glycines parasitism proteins. Phytopathology 93:S23.
May, K.J., T. K. Mitchell, and E. L. Davis. 2003. Towards development of bacterial artificial chromosome (BAC) libraries for plant parasitic nematodes. Phytopathology 93:S59.
SC - Publications:
Shipe, E.R., J.D. Mueller, S.A. Lewis, and R.K. Stephens. 2003. Conventional and Roundup Ready Soybean Development at Clemson. Proceedings of the 2003 AG EXPO, Feb. 3-5. Myrtle Beach, SC
Shipe, E.R., J.D. Mueller, S.A. Lewis, P.F. Williams, Jr., and R.K. Stephens. 2003. Registration of ‘Santee‘ soybean. Crop Science 43(6) Nov.-Dec. In press.
Robbins, R.T., E.R. Shipe, L. Rakes, L.E. Jackson, E.E. Gbur, and D.G. Dombek. 2002. Host suitability of soybean cultivars and breeding lines to reniform nematode in tests conducted in 2001. J. of Nematology 34(4): 378-383.
TX - publications
Simpson, C. E., J. L. Starr, G. T. Church, M. D. Burow, and A. H. Paterson. 2003. Registration of NemaTAM peanut. Crop Science 43:1561.
Bendezu, I. F., and J. L. Starr. 2003. Mechanism of resistance to Meloidogyne arenaria in the peanut cultivar COAN. Journal of Nematology 35:115-118.
Church, G., J. L. Starr, and C. E. Simpson. 2003. The identification and development of resistance to Meloidogyne arenaria in groundnut. Proc. 4th International Nematology Congress, Tenerife, Spain.
Silvey, D. T., K. Ripple, C. W. Smith, and J. L. Starr. 2003. Identification of RFLP loci linked to resistance to Meloidogyne incognita and Rotylenchulus reniformis. Beltwide Cotton Conferences (Abstr.).
Church, G. T., J.L. Starr, and C. E. Simpson. 2003. Resistance to Meloidogyne in Arachis hypogaea: History and future. Journal of Nematology 35:330 (Abstr.).
VA - Publications
Crowder*, B. J., C. A. Wilkinson, C. S. Johnson, and J. D. Eisenback. 2003. Inheritance of resistance to tobacco cyst nematode in flue-cured tobacco. Crop Science 43:1305-1312.
Johnson, C. S. 2003. Contemporary issues in managing tobacco nematodes. Pan American Plant Disease Conference (Joint meeting of the Caribbean and Southern Divisions of the American Phytopathological Society with the Latin American Association of Phytopathology and the Mexican Phytopathological Society), South Padre Island, TX, April 5-10, 2003.
C.S. Johnson, and L. Wright. 2003. Blue mold severity on selected burley tobacco cultivars in Virginia, with and without Acrobat, 2002. B&C Tests 18:F028.
C.S. Johnson, C.A. Wilkinson, and L. Wright. 2003. Blue mold severity in the Virginia official variety test for burley tobacco, 2002. B&C Tests 18:F029.
C.S. Johnson, D.R. Peek, and R.D. Miller. 2003. Blue mold severity on selected entries of burley tobacco in Virginia, 2002. B&C Tests 18:F030.
C.S. Johnson, and C. T. Clarke. 2003. Host resistance and chloropicrin to control Granville wilt in Virginia, 2002. F&N Tests 58:FC049.
C.S. Johnson, and C. T. Clarke. 2003. Host resistance and Telone C-17 to control tobacco cyst nematodes in Virginia, 2002. F&N Tests 58:N010.
C.S. Johnson, and W.B. Wilkinson, III. 2003. Host resistance and use of Telone products to control tobacco cyst nematodes in Virginia, 2002. F&N Tests 58:011.
C.S. Johnson, C. T. Clarke. 2003. Host resistance and nematicides to control tobacco cyst nematodes in Virginia, 2002. F&N Tests 58:N012.
Johnson, C. S., D. Faustini, D. G. Schaheen, P. J. Semtner, J. W. Walton, P. K. Blevins, J. Hartlage, R. M. Huckaba, and G. Roberson. 2003. Fumigation of Soil and Agricultural Products: A Guide for Virginia Pesticide Applicators. Virginia Department of Agriculture and Consumer Services. 212pp.
Johnson, C. S. 2003. Flue-Cured Tobacco Production Guide. Disease Management.
Publ. 436-050.
Johnson, C. S. 2003. Flue-Cured Tobacco Production Guide. Weed Management. Publ. 436-050.
Johnson, C. S. 2003. Pest Management Guide for Field Crops 2003. Diseases of Tobacco. Publ. 456-016.
Johnson, C. S. 2003. Pest Management Guide for Field Crops 2003. Weed Control in Tobacco. Publ. 456-016.
Peek, D. R., C. A. Wilkinson, T. D. Reed, and C. S. Johnson. 2003. Burley tobacco variety information for 2003. Publ. 436-417.
Wilkinson, C. A., T. D. Reed, C. S. Johnson, and J. L. Jones. 2003. Flue-cured tobacco variety information for 2003. Publ. 436-047.
Wilkinson, C. A., T. D. Reed, and C. S. Johnson. 2003. Virginia dark-fired tobacco variety information for 2003. Publ. 436-418.
Diez, Asdrubal, G. W. Lawrence and K. S. McLean. 2003. Nematode post-infection development on cotton following separate and concomitant parasitism by Meloidogyne incognita and Rotylenchulus reniformis. Journal of Nematology (In Press).
McLean, K. S. and G. W. Lawrence. 2003. Efficacy of Aldicarb to Rotylenchulus reniformis and biodegradation in cotton field soils. Journal of Nematology 35 (1):65-72.
Lawrence, G. W. and K. S. McLean. 2003. Foliar applications of oxamyl with aldicarb for the management of Meloidogyne incognita on cotton. Nematropica Vol 32, No. 2:103-112.
McLean, K. S. and C. Dale Monks. Eds. 2002 Cotton Research Report. March 2003. Alabama Agricultural Experiment Station Research Report Series No. 24. Pp 52.
McLean, K. S. G. W. Lawrence, C. Overstreet, and L. D. Young. 2003. Efficacy of anhydrous ammonia on the reniform nematode in cotton. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:282-283 (2003) National Cotton Council, Memphis TN.
Usery, S. R., K. S. McLean, C. Burmester, E. Van Saten, and Brad Meyer. 2003. Response of selected cotton varieties to the reniform nematode in Alabama. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:261-264 (2003) National Cotton Council, Memphis TN.
Hutchinson, J. L., J. R. Jones, K. S. McLean, and J. Williams. 2003. Evaluation of selected cover crops to determine host suitability for Rotylenchulus reniformis. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:248-249 (2003) National Cotton Council, Memphis TN.
Palmateer, A. J., K. S. McLean, and E. Van Saten. 2003. Interaction of Rotylenchulus reniformis with seedling disease pathogens of cotton. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:222-225 (2003) National Cotton Council, Memphis TN.
Tagert, M. D., G. W. Lawrence, W. E. Batson, H. K. Lee, A. T. Kelly, and K. S. McLean. 2003. Severity of disease caused by Thielaviopsis basicola and Rotylenchulus reniformis in concomitant or sequential interactions. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:147-148 ( 2003) National Cotton Council, Memphis TN.
Tagert, M. D., G. W. Lawrence, W. E. Batson, H. K. Lee, A. T. Kelly, and K. S. McLean. 2003. Thielaviopsis basicola and Rotylenchulus reniformis as independent or interactive pathogens on cotton in different soil types. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:145-146 ( 2003) National Cotton Council, Memphis TN.
Usery, S. R., K. S. McLean, C. Burmester, E. Van Saten, and Brad Meyer. 2003. Response of selected cotton varieties to the reniform nematode in Alabama. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:261-264 (2003) National Cotton Council, Memphis TN.
Hutchinson, J. L., J. R. Jones, K. S. McLean, and J. Williams. 2003. Evaluation of selected cover crops to determine host suitability for Rotylenchulus reniformis. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:248-249 (2003) National Cotton Council, Memphis TN.
Palmateer, A. J., K. S. McLean, and E. Van Saten. 2003. Interaction of Rotylenchulus reniformis with seedling disease pathogens of cotton. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:222-225 (2003) National Cotton Council, Memphis TN.
Tagert, M. D., G. W. Lawrence, W. E. Batson, H. K. Lee, A. T. Kelly, and K. S. McLean. 2003. Severity of disease caused by Thielaviopsis basicola and Rotylenchulus reniformis in concomitant or sequential interactions. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:147-148 ( 2003) National Cotton Council, Memphis TN.
Tagert, M. D., G. W. Lawrence, W. E. Batson, H. K. Lee, A. T. Kelly, and K. S. McLean. 2003. Thielaviopsis basicola and Rotylenchulus reniformis as independent or interactive pathogens on cotton in different soil types. (online) Proceedings of the National Beltwide Cotton Conference Vol. 1:145-146 ( 2003) National Cotton Council, Memphis TN.
Hutchinson, J. L., K. S. McLean, Y. Feng, C. Burmester, and G. W. Lawrence. 2003. Accelerated breakdown of aldicarb in Alabama cotton field soil. Phytopathology 93:S38 Publication no. P-2003-0274-AMA.
Lawrence, G. W., A. T. Kelley, H. K. Lee, W. A. Giverns, and K. S. McLean. 2003. Site specific management of the reniform nematode on cotton. Phytopathology 93:S48 Publication no. P-2003-0353-AMA.
Palmateer, A.J., K.S. McLean, J. D. Mayfield, and G. Morgan-Jones. 2003. Characterization of a Phomopsis species occurring on cotton with comparison to species colonizing soybean. Phytopathology 93:S69 Publication no. P-2003-0500-AMA.
Lawrence, G. W., A. T. Kelley, H. K. Lee, K. S. McLean. 2003. Evaluation of Adage 5FS for control of Rotylenchulus reniformis on cotton in the Mississippi delta, 2000. Fungicide and Nematicide Tests (online.) Report 58:N004. DOI: 10.1094/FN58. The American Phytopathological Society, St. Paul, MN.
Lawrence, G. W., A. T. Kelley, H. K. Lee, K. S. McLean. 2003. Evaluation of Telone II for control of Rotylenchulus reniformis on cotton in the Mississippi delta, 2000. Fungicide and Nematicide Tests (online.) Report 58:N005. DOI: 10.1094/FN58. The American Phytopathological Society, St. Paul, MN.
Lawrence, G. W., A. T. Kelley, H. K. Lee, K. S. McLean. 2003. Evaluation of Nemacur 240CS for control of Rotylenchulus reniformis on cotton in Mississippi, 2000. Fungicide and Nematicide Tests (online.) Report 58:N006. DOI: 10.1094/FN58. The American Phytopathological Society, St. Paul, MN.
Lawrence, G. W., A. T. Kelley, H. K. Lee, K. S. McLean. 2003. Evaluation of Telone II for control of Meloidogyne incognita in ultra narrow row cotton in Mississippi, 2000. Fungicide and Nematicide Tests (online.) Report 58:N007. DOI: 10.1094/FN58. The American Phytopathological Society, St. Paul, MN.
Lawrence, G. W., A. T. Kelley, H. K. Lee, K. S. McLean. 2003. Evaluation of Temik 15G for control of Rotylenchulus reniformis in the Mississippi delta, 2000. Fungicide and Nematicide Tests (online.) Report 58:N008. DOI: 10.1094/FN58. The American Phytopathological Society, St. Paul, MN.
McLean, K. S., A. J. Palmateer, J. L. Hutchinson, J. Jones, G. W. Lawrence. 2003. Evaluation of Messenger with Temik 15G for control of Rotylenchulus reniformis on cotton in central Alabama, 2002. Fungicide and Nematicide Tests (online.) Report 58:N009. DOI: 10.1094/FN58. The American Phytopathological Society, St. Paul, MN.
Usery, S. R. Jr., K. S. McLean, C. B. Burmester, B. A. Meyer, E. van Santen. 2003. Transgenic cotton variety response to Rotylenchulus reniformis in a corn cotton rotation in North Alabama, 2002. Biological and Cultural Tests for Control of Plant Diseases (online.) Report No. 18:F008. DOI: 10.1094/BC18. The American Phytopathological Society, St. Paul, MN.
Usery, S. R. Jr., K. S. McLean, C. B. Burmester, B. A. Meyer, E. van Santen. 2003. Transgenic cotton variety response to Rotylenchulus reniformis in North Alabama, 2002. Biological and Cultural Tests for Control of Plant Diseases (online.) Report No. 18:F009. DOI: 10.1094/BC18. The American Phytopathological Society, St. Paul, MN.
LA Publications
C. Overstreet, E.C. McGawley and B. Padgett. 2002. Current management strategies employed against the reniform nematode (Rotylenchulus reniformis) in cotton production in Louisiana, U.S.A Nematology 4: 306-307.
Erwin, T.L., C. Overstreet, and E.C. McGawley. 2002. Nematicides against reniform nematode in Louisiana. Proceedings of the Beltwide Cotton Conferences, CD Rom, National Cotton Council, P.O. Box 820285, Memphis, TN.
Overstreet C. and E.C. McGawley. 2002-Workshop-Nematode Management: reniform/root-knot competition and reproduction. Proceedings of the Beltwide Cotton Conferences, CD Rom, National Cotton Council, P.O. Box 820285, Mamphis, TN.
Lewis, S. A., D. J. Chitwood, and E. C. McGawley. 2003. Nematode Biology, Morphology and Physiology in Dekker Encyclopedia of Plant and Crop Science, Robert M. Goodman, ed. Marcel Dekker, NY (in press).
Carter-Wientjes, C.H., J.S. Russin, D.J. Boethel, J.L. Griffin and E.C. McGawley. 2003. Feeding and Maturation by Soybean Looper (Lepidoptera: Noctuidae) Larvae on Soybean Affected by Weed, Fungus, and Nematode Pests. Journal of Economic Entomology (in press).
Bond, J.P., E.C. McGawley and J.W. Hoy. 200?. Interaction of nematodes and Pythium spp. on Sugarcane in Louisiana. Phytopathology (submitted).
MN -Publications:
Chen, S. Y., and F. J. Chen. 2003. Fungal parasitism of Heterodera glycines eggs as influenced by egg age and pre-colonization of cysts by other fungi. Journal of Nematology 35(3):
Liu, X. Z., and S. Y. Chen. 2003. Nutritional requirements of the ARF18 and Pochonia chlamydospora, fungal parasites of nematode eggs. Journal of Invertebrate Pathology 83:10-15.
Chen, S. Y., J. H. Orf, D. R. Miller, and P. J. Schaus. 2003. Performance of soybean varieties in SCN-infested and non-infested fields in Minnesota during 2002. http://sroc.coafes.umn.edu/Nematology/PDF%20Files/Variety%202002-1.pdf
Chen, S. Y., and D. W. Dickson. 2003. Biological control of nematodes by fungal antagonists. Pp. 343-403 in Z. X. Chen, S. Y. Chen, and D. W. Dickson, eds. Nematology: Advances and perspectives. Vol. 2. Nematode management and utilization. Beijing: Tsinghua University Press and CAB International. (in press)
Chen, Z. X., S. Y. Chen, and D. W. Dickson (eds.). 2003. Nematology, advances and perspectives. Vol. 1. Nematode morphology, physiology and ecology. Beijing: Tsinghua University Press and CAB International.
Chen, S. 2003. Biocontrol potential of the fungi Hirsutella rhossiliensis and H. minnesotensis against Heterodera glycines. Phytopathology 93:S123.
MS - Publications:
Lawrence, G.W., A.T. Kelley, R. L. King, J. Vickery, H.K. Lee, and K. S. McLean. 2004. Remote sensing and precision nematicide applications for Rotylenchulus reniformis management in Mississippi cotton. Nematology Monographs and Perspectives 2: (accepted as a manuscript to be published as part of an invited symposium. All manuscripts are awaiting the final approval from the editorial board).
Lawrence, G.W., S. Samson, H.K. Lee, A.T. Kelley, and W.A. Givens. 2003. Reniform nematode management with variable rate nematicide applications. Proceedings of the National Beltwide Cotton Conference, Vol. 1:273-274 (2003) National Cotton Council, Memphis TN.
H.K. Lee, G.W. Lawrence, J.L. DuBien, and A.T. Kelley. 2003. Seasonal variation in the spatial distribution of Rotylenchulus reniformis in Mississippi cotton. Proceedings of the National Beltwide Cotton Conference, Vol. 1: 245-246 (2003) National Cotton Council, Memphis
Kelley, A.T., G.W. Lawrence, R. L. King, and J. Vickery. 2003. Spectral classification of reniform nematode (Rotylenchulus reniformis) infected cotton plants. International Symposium on Remote Sensing of Environment. (Accepted for publication).
Kelley, A.T., G.W. Lawrence, J. Vickery, R. King, and H.K. Lee. 2003. Detection and population estimation of the reniform nematode on cotton using hyperspectral remotely sensed data. Proceedings of the National Beltwide Cotton Conference Vol. 1:241-244 (2003) National Cotton Council, Memphis TN.
Kelley, A.T., G.W. Lawrence, R.L. King, J. Vickery, and H.K. Lee. 2003. Spectral Classification of Reniform Nematode (Rotylenchulus reniformis) Infested Cotton Plants Using Self-Organized Maps. Journal of Nematology 32: (abstract).
Lee, H.K., G.W. Lawrence, J.L. DuBien, and A.T. Kelley. 2003. Cultural Practices and the Dispersion of the Reniform Nematode In Mississippi. Journal of Nematology 32: (abstract).
H.K. Lee, G.W. Lawrence, and A.T. Kelley. 2003. Seasonal variation in the spatial distribution of Rotylenchulus reniformis in Mississippi cotton. Phytopathology 93: S49 Publication P-2003-0358-AMA.
Vizzier-Thaxton, Y., G.W. Lawrence and C.L. Balzli. 2002. Cotton nematode control by broiler litter. Poultry Science (Suppl.): 81:55.
NC Publications:
Cervantes-Flores, J.C., G.C. Yencho, and E.L. Davis. 2002. Host reactions of sweetpotato genotypes to root-knot nematodes and variation in virulence of Meloidogyne incognita populations. HortScience 37(7): 1112-1116.
Davis, R. F., S. R. Koenning, R. C. Kemerait, T.D. Cummings, and W. D. Shurley. 2003. Rotylenchulus reniformis management in cotton with crop rotation. Journal of Nematology 35:58-64.
Gao, B., R. Allen, T. Maier, E. L. Davis, T. J. Baum, and R. S. Hussey. 2003. The parasitome of the phytonematode Heterodera glycines. Molecular Plant-Microbe Interactions 16:270-276.
Huang, G., B. Gao, T. Maier, R. Allen, E.L. Davis, T.J. Baum, and R.S. Hussey. 2003. A profile of putative parasitism genes expressed in the esophageal gland cells of the root-knot nematode, Meloidogyne incognita. Molecular Plant-Microbe Interactions 16: 376-38
Koenning, S. R., K. L. Edmisten, K. R. Barker, and D. E. Morrison. 2003. Impact of cotton production system on Hoplolaimus columbus. Journal of Nematology 35:73-77.
Koenning, S. R., K. R. Barker, K. L. Edmisten, D. T. Bowman, and D. E. Morrison. 2003. Effects of rate and time of application of poultry litter on Hoplolaimus columbus on cotton. Plant Disease 87:1244-1249.
Wrather, J. A., S. R. Koenning, and T. Anderson. 2003. Effect of diseases on soybean yields in the United States and Ontario 1999-2002. Online. Plant Health Progress doi: 10.1094/PHP-2003-0325-01-RV. http://www.plantmanagementnetwork.org/pub/php/review/2003/soybean/.
Hussey, R.S., E.L. Davis, and T.J. Baum. 2002. Secrets in secretions: genes that control nematode parasitism of plants. Brazilian Journal of Plant Physiology 14:183-194.
Baum, T.J., Hussey, R.S., and Davis, E.L. 2003. Secrets in Secretions: Analysis of the Parasitome of Heterodera glycines. IX Meeting of the International Society for Molecular Plant-Microbe Interactions, St. Petersburg, Russia.
Sukno, S., O. Shoseyov, O. Shimerling, and E. Davis. 2003. Expression of truncated Arabidopsis thaliana Cel1 promoter during compatible plant-nematode interactions. Phytopathology 93:S81.
Huang, G., R. Dong, T. Maier, R. Allen, E. Davis, T. Baum, and R. Hussey. 2003. An efficient subtractive strategy for the identification of parasitism genes of Meloidogyne incognita. Phytopathology 93:S37.
Wang, X., Allen, R., Maier, T., Hussey, R.S., Baum, T.J., and Davis, E.L. 2003. Functional analysis of a parasitism gene from Heterodera glycines with similarity to CLAVATA3 of Arabidopsis thaliana. Phytopathology 93:S90.
Diab, H.G., B. Gao, T. J. Baum, R. S. Hussey, and E. L. Davis. 2003. Immunodetection of parasitism proteins in Heterodera glycines. Phytopathology 93:S21.
Wong, M. -Y., E. L. Davis, and J. -S. Huang. 2003. Nitric oxide synthase in higher plants: An attempt to clone the gene. Phytopathology 93:S91.
Elling, A.A., B. Gao, R. S. Hussey, E. L. Davis, and T. J. Baum. 2003. Plant nuclear import of Heterodera glycines parasitism proteins. Phytopathology 93:S23.
May, K.J., T. K. Mitchell, and E. L. Davis. 2003. Towards development of bacterial artificial chromosome (BAC) libraries for plant parasitic nematodes. Phytopathology 93:S59.
SC - Publications:
Shipe, E.R., J.D. Mueller, S.A. Lewis, and R.K. Stephens. 2003. Conventional and Roundup Ready Soybean Development at Clemson. Proceedings of the 2003 AG EXPO, Feb. 3-5. Myrtle Beach, SC
Shipe, E.R., J.D. Mueller, S.A. Lewis, P.F. Williams, Jr., and R.K. Stephens. 2003. Registration of ‘Santee‘ soybean. Crop Science 43(6) Nov.-Dec. In press.
Robbins, R.T., E.R. Shipe, L. Rakes, L.E. Jackson, E.E. Gbur, and D.G. Dombek. 2002. Host suitability of soybean cultivars and breeding lines to reniform nematode in tests conducted in 2001. J. of Nematology 34(4): 378-383.
TX - publications
Simpson, C. E., J. L. Starr, G. T. Church, M. D. Burow, and A. H. Paterson. 2003. Registration of NemaTAM peanut. Crop Science 43:1561.
Bendezu, I. F., and J. L. Starr. 2003. Mechanism of resistance to Meloidogyne arenaria in the peanut cultivar COAN. Journal of Nematology 35:115-118.
Church, G., J. L. Starr, and C. E. Simpson. 2003. The identification and development of resistance to Meloidogyne arenaria in groundnut. Proc. 4th International Nematology Congress, Tenerife, Spain.
Silvey, D. T., K. Ripple, C. W. Smith, and J. L. Starr. 2003. Identification of RFLP loci linked to resistance to Meloidogyne incognita and Rotylenchulus reniformis. Beltwide Cotton Conferences (Abstr.).
Church, G. T., J.L. Starr, and C. E. Simpson. 2003. Resistance to Meloidogyne in Arachis hypogaea: History and future. Journal of Nematology 35:330 (Abstr.).
VA - Publications
Crowder*, B. J., C. A. Wilkinson, C. S. Johnson, and J. D. Eisenback. 2003. Inheritance of resistance to tobacco cyst nematode in flue-cured tobacco. Crop Science 43:1305-1312.
Johnson, C. S. 2003. Contemporary issues in managing tobacco nematodes. Pan American Plant Disease Conference (Joint meeting of the Caribbean and Southern Divisions of the American Phytopathological Society with the Latin American Association of Phytopathology and the Mexican Phytopathological Society), South Padre Island, TX, April 5-10, 2003.
C.S. Johnson, and L. Wright. 2003. Blue mold severity on selected burley tobacco cultivars in Virginia, with and without Acrobat, 2002. B&C Tests 18:F028.
C.S. Johnson, C.A. Wilkinson, and L. Wright. 2003. Blue mold severity in the Virginia official variety test for burley tobacco, 2002. B&C Tests 18:F029.
C.S. Johnson, D.R. Peek, and R.D. Miller. 2003. Blue mold severity on selected entries of burley tobacco in Virginia, 2002. B&C Tests 18:F030.
C.S. Johnson, and C. T. Clarke. 2003. Host resistance and chloropicrin to control Granville wilt in Virginia, 2002. F&N Tests 58:FC049.
C.S. Johnson, and C. T. Clarke. 2003. Host resistance and Telone C-17 to control tobacco cyst nematodes in Virginia, 2002. F&N Tests 58:N010.
C.S. Johnson, and W.B. Wilkinson, III. 2003. Host resistance and use of Telone products to control tobacco cyst nematodes in Virginia, 2002. F&N Tests 58:011.
C.S. Johnson, C. T. Clarke. 2003. Host resistance and nematicides to control tobacco cyst nematodes in Virginia, 2002. F&N Tests 58:N012.
Johnson, C. S., D. Faustini, D. G. Schaheen, P. J. Semtner, J. W. Walton, P. K. Blevins, J. Hartlage, R. M. Huckaba, and G. Roberson. 2003. Fumigation of Soil and Agricultural Products: A Guide for Virginia Pesticide Applicators. Virginia Department of Agriculture and Consumer Services. 212pp.
Johnson, C. S. 2003. Flue-Cured Tobacco Production Guide. Disease Management.
Publ. 436-050.
Johnson, C. S. 2003. Flue-Cured Tobacco Production Guide. Weed Management. Publ. 436-050.
Johnson, C. S. 2003. Pest Management Guide for Field Crops 2003. Diseases of Tobacco. Publ. 456-016.
Johnson, C. S. 2003. Pest Management Guide for Field Crops 2003. Weed Control in Tobacco. Publ. 456-016.
Peek, D. R., C. A. Wilkinson, T. D. Reed, and C. S. Johnson. 2003. Burley tobacco variety information for 2003. Publ. 436-417.
Wilkinson, C. A., T. D. Reed, C. S. Johnson, and J. L. Jones. 2003. Flue-cured tobacco variety information for 2003. Publ. 436-047.
Wilkinson, C. A., T. D. Reed, and C. S. Johnson. 2003. Virginia dark-fired tobacco variety information for 2003. Publ. 436-418.