Brief Summary of Minutes of Annual Meeting

The NC-1008 meeting (the meeting formerly known as NC-168) was held in conjunction with the Plant & Animal Genome XII meeting at the Town & Country Resort in San Diego, CA. The meeting was called to order on Saturday at 12:45 by NC-1008 chair, Bill Muir.

Following organization comments by the chair and self-introductions by the attendees, reports were presented as published in the PAG XII program so that others would know when speakers were scheduled. Objective coordinators were the same as last year: Mary Delany ?Objective 1; Jerry Dodgson?Objective 2; and Chris Ashwell?Objective 3. The speakers on Saturday included: Martien Groenen (guest; Netherlands); Jerry Dodgson (MI and NRSP-8 Coordinator); Susan Lamont and Jack Dekker (IA); Hans Cheng (USDA-ARS;ADOL); Mary Delany (CA-UC Davis); Tom Porter (MD); Bill Muir (IN). Presentations continued on Sunday with Larry Cogburn (guest); Marlene Emara (DE); James Zhu (TX); David Burt (guest); Wayne Kuenzel and Ron Okimoto (AR); Tom Wicker (guest); Marcia Miller (CA-City of Hope); Douglas Foster and Kent Reed (MN); Jim Petitte (NC); Urs Kuhnlein (guest) and Chris Ashwell (USDA-ARS;GBL).

The business meeting was called to order by Bill Muir. Sue Lamont moved to approve the minutes (seconded by Hans Cheng) for the 2002 NC-168 meeting. Margaret Dentine, our Administrative Advisor, noted that both NC-1008 and NRSP-8 annual reports should be posted on the NIMSS web site within 60 days after the meeting and reported that NC-1008 has an active program and should continue to emphasize the collaborative nature of our work. She informed members that changes in affiliation, deletions and new membership additions now reside in NIMSS with yearly updates.

Regarding admission of new members, Jerry Dodgson moved (seconded by Mary Delany) that Margaret should act on requests for admission and the committee would be notified of those actions. With urging from others, Marlene Emara agreed to coordinate the writing of the final report for the last five years for NC-168. Muquarrab Qureshi (Animal Genetics National Program Leader) gave updates for the NRICRG funding. Sue Lamont moved (seconded by Jerry Dodgson) that all reports be sent electronically to both NC-1008 and NRSP-8 individuals and administrators by the Wednesday before the next annual meeting, the motion was carried.

The motion was made by Sue Lamont and seconded by Hans Cheng to have the next NC-1008 meeting in conjunction with PAG XIII on January 15-16, 2005, and to maintain Bill Muir?s organizational style (invited speakers, titles organized by topic, and 30 min presentations including questions, unless additional time requested, a maximum of 45 minutes would be allocated to any one station) and was agreed unanimously.

The starting time for next year?s meeting will be 12 noon on Saturday January 15 and the participants agreed to email the chair a month before the meeting the following: 1) title of talk; 2) time requested for talk; and 3) desired time of presentation. The chair would make final adjustments to time and order of presentation as needed.

Kent Reed was nominated and accepted the position as NRSP-8 secretary for next year and Tom Porter was nominated and approved (since he wasn?t at the meeting) as NC-1008 secretary for next year.

Chris Ashwell will be the Chair of NRSP-8 and meeting coordinator for next year and Doug Foster will be Chair NC-1008 next year and coordinate the meeting the following year. It was agreed as a standard operating procedure that the chairs of the NRSP-8 and NC-1008 committees would alternate as coordinators. Only the coordinator would receive funds for travel from the PAG organizers. The organizers offer $100 off the registration cost for up to 6 invited speakers.

To be eligible for the discount the speaker must 1) register through the PAG speaker web site, 2) must register for the entire PAG program, 3) must submit an abstract, and 4) the names must be sent by the coordinator of the Poultry Workshop to the Coordinator of PAG.

It was agreed that these would be offered first to officers of the Poultry Workshop and if declined would be offered to invited speakers. The objective coordinators will remain the same (Objective 1: Mary Delany; Objective 2: Jerry Dodgson; and Objective 3: Chris Ashwell).

The roles of the officers in the reporting process were outlined as follows: the objective coordinators would compile material from individual reports into an objective report and forward to the secretary. The secretary would keep the minutes of the meeting and in addition collect the coordinators reports (prodding as needed) and add publications reported from all stations. The secretary would forward those items to the NC-1008 chair, within 60 days of the meeting, who would review and approve the minutes and report before forwarding to the administrative advisor.

Bill Muir was complimented on his organization of what was the best NC-1008 meeting yet.

The meeting was adjourned almost on time at 5:45.

ACCOMPLISHMENTS AND IMPACTS:

Objective 1. Utilize Modern Molecular and Breeding Technologies to Identify, Locate, Isolate and Characterize Poultry Gene of Economic Importance.

Progress was achieved in many areas of poultry (chicken and turkey) genetic and genomic analysis involving the alignment and enhancement of the genetic, physical and cytogenetic maps, in developing the various maps and sequence information for genes/QTLs of economically relevant traits, and in the identification of gene expression profiles. Advances involved substantial collaborative research among technical committee members and included sharing of information, reagents, tools and genetic resources. Results from the previous year included: Feed efficiency studies were conducted by AR using birds having variant forms of the UCP, COII cytb and POMC genes. Segregating mitoD-loop types having different feed efficiencies were found to vary for a substitution in the COII gene; Feather color studies continued for the purpose of developing pooled multiplexed sequencing tests for E locus alleles, recessive white and dominant white. Work progressed on the stereotaxic atlas of the chick brain wherein 50 vector-based plates were completed to date. The plates will allow users to create customized diagramming for research and teaching. CA (Delany) examined profiling of genes regulating telomerase genes and telomere access, which impact genome stability by using quantitative RT-PCR. Several cell systems are under evaluation. An inheritance study was initiated to evaluate telomere recombination using UCD003. CA (Miller) continued work to elucidate the MHC-Rfp-Y gene region. Numerous other genes, including a series of c-lectin-like genes were identified within the various clones. Work was conducted to define break-points within MHC recombinants indicating recombination occurred at various points within a potential hotspot region as well as outside the region. Characterization was initiated by DE for a family of genes known as the F-box protein family; these genes are involved in degradation and control of cell cycle and other regulatory proteins. FBX07 mapped to Gga 1 in the region of DEL001. Sequencing was performed and exon/intron structure was determined. Twenty-one members of the F-box family were identified from chicken ESTs. Studies continued by IA utilizing the Iowa Growth and Composition Resource Population to identify genes associated with skeletal integrity. Candidate genes included BMP6, BMP-receptor 1, Vit D receptor, insulin, among others. BMP6 SNPs were significantly associated with most skeletal traits. Microarray (chicken lymphoid cDNA, 2880 genes) gene expression studies were initiated to assess global gene expression of Leghorn, Fayoumi and broiler lines. No significant variation was identified for the microarray system (activated T cells, mixed lymphoid tissue library) and organ/age sampled (adult spleen). A chicken cDNA microarray (5K array) for the neuroendocrine system was created previously by MD (USDA-IFAFS funds). Initial characterization included gene expression among tissues of the neuroendocrine system (anterior pituitary, pineal gland, and hypothalamus) to assess tissue-specific and shared expression profiles. In all, 426 restricted genes and 4,107 shared genes were found expressed among the three tissues. A developmental profile of gene expression of the neuroendocrine system was examined comparing the late embryonic and neonatal stages indicating activation of such genes as the myelin basic protein, prolactin and decreases in message for type 2 somatostatin. Work continued by MI (Dodgson) on the development and characterization of large insert BAC libraries for the research community; DNA from the female RJF UCD001 chicken used to create the TAMU and CHORI libraries was provided to the Washington U. Genome Center to construct small plasmid libraries for whole genome shotgun sequencing. Overgo hybridization was utilized to assign over 5700 BACs to over 720 markers or genes to integrate the BAC contigs to the existing genetic map. Linkage of the physical map to the genetic marker map is a key component for contig and sequence assembly. The E.Lansing linkage map was expanded to 1249 markers by MI (Cheng); of the 19 new markers, 11 were genes (8 mapped as SNPs) and 8 were SNPs to anonymous sequences. Research by MN emphasized microsatellite marker development and linkage mapping in the turkey. A total of 397 turkey microsatellite markers were developed from small insert libraries. Additionally, to identify suitable markers for mapping, PCR amplification of 772 markers (chicken, quail and turkey) led to the testing of 410 markers for polymorphism in the resource families chosen for linkage mapping. Twenty-six percent were polymorphic and were tested for informativeness and genotyped in the mapping families. All information is available at http://www.tc.umn.edu/~reedx054/Turkeygenome.htm). Approximately 140 RFLP markers from an embryonic cDNA library were mapped in turkey; additional clones were sequenced as a SNP resource. Nucleotide sequencing of the avian pneumovirus (APV) was conducted and indicates substantial nucleotide and amino acid identity between strains isolated from 1996 to 1999. Phylogenetic relationships between European and US strain were established. The sequence of the entire APV/C virus was completed. Work continued to evaluate the host transcriptional response to APV infection indicating upregulation of transcripts involving leukocyte chemoattractants, adhesion molecules and complement as well as genes involved in protein trafficking pathways. RNA expression of novel chicken interleukins, beta-defensins and toll-like receptors was investigated by TX (via real time-PCR) in broilers and layers following LPS stimulation (before/after study). Liver, spleen and lymphocyte samples were studied. Overall the collective data suggest that broilers may have a lowered innate immune response as compared to layers.

Objective 2. Develop Methods for Creating New Genetic Variation in Poultry by Gene Transfer and Chromosome Alteration.

Reports were provided by MI, MN and NC, with collaboration with CA and USDA-ARS ADOL (ADOL). Viral-based transgenic technologies. MI has begun to test VSV-pseudotyped one-round viral vectors for creating transgenic chickens. MI is also using cloned ALV vectors in combination with cloned MAV for biochemical scale production of proteins in vivo in infected chickens. NC continues characterization and breeding of a transgenic line produced with a replication-defective spleen necrosis virus vector that expresses beta-galactosidase. This line is now at the G3 stage and has shown the expected Mendelian inheritance. Physiological studies on the line have begun. Blastodermal and stem cell culture and analysis of germline commitment. NC continues to examine the expression of DAZL and VASA genes in germline cells. The purpose is to distinguish between an inductive mode or a pre-determined mode of germ cell specification. MI concluded collaborative studies with ADOL on the use of chimeric chicken techniques and blastodermal cell culturing to generate germline transgenic birds. The effects of growth factor extracts on cell growth and telomere maintenance were performed in collaboration with ADOL and CA. MN has recently isolated chicken stem cell-like cells from 6 day-old SPAFAS chicken embryos and is currently investigating what growth factors and media conditions will maintain a viable stem cell population without the use of heterologous feeder layers. Sperm-mediated gene transfer (SMGT). BioAgri Corp. reported successful SMGT with several agricultural animal species including chickens. MI and ADOL obtained reagents from BioAgri in an attempt to duplicate their results. Rigorous tests of SMGT using DNA that encodes the RCASBP(A) replication competent avian leukosis virus (ALV) retroviral vector were performed. Despite every attempt to reproduce the BioAgri conditions, SMGT has not been successful in their hands. Studies of immortal avian cell lines. MN has succeeded in developing the world?s first and only non-virally immortalized continuously growing turkey cell line (TT-1). MN has ascertained that this new turkey-specific cell line supports the propagation of avian pneumovirus (APV).

Objective 3. Develop, Compare and Integrate Emerging Technologies with Classical Quantitative Genetics for Improvement of Economic Traits in Poultry.

IN used compute simulations to examine Genome wide MAS. For all combinations of parameters examined, a small population size was better than large. For a poultry breeder this does not mean that inbreeding will be a problem, rather the breeding structure must change to utilize this method. IN showed that a subdivide merge scheme of breeding can be utilized. With this method the population is divided into many small population, selection occurs within line and periodically the lines are crossed and re-subdivided. The rate of inbreeding is the same as for one large population of similar cumulative size. ADOL and IA continued previous work to identify QTL conferring resistance to Marek?s disease in commercial layers was performed by both. Previously, using the Marek?s disease (MD) resistant inbred Line 6 and the MD susceptible Line 7 to generate a F2 progeny resource population, 14 QTL were identified conferring susceptibility to Marek?s disease. To verify if the QTL that was identified in experimental crosses existed in commercial chickens, and to identify new QTL, two resource populations were developed in collaboration with Hy-Line. For genotyping, ~120 informative microsatellite markers have been scored on the parents, F1 sires, and selective DNA pools (within MHC haplotype and cross) for all the BC progeny. Statistical analyses identified many markers that show suggestive association with disease resistance traits in one or more analyses. ADOL also continued efforts to identify candidates for genetic resistance to Marek?s disease (MD) using a comprehensive two-hybrid screen was conducted using genes unique to virulent Marek?s disease virus (MDV) strains. ADOL also validated a SNP protocol for assessment of chicken genotypes conferring susceptibility or resistance to infection by subgroup E and subgroup B avian leukosis viruses (ALV). A comprehensive scan of the chicken genome using microsatellite markers was carried out on a meat-type x egg-type resource population (wide cross) by ADOL. USDA-ARS,GBL (GBL), in collaboration with IA, has completed genotyping the F2 generation of the Iowa Growth and Composition Resource Population (IGCRP). A genome scan was conducted and included 269 microsatellite markers. Initial analysis of data from GGA1 by interval mapping suggests the presence of QTL for age dependent growth related traits. IA and GBL also jointly collected data for nutrient absorption/retention phenotypes for the IGCRP advanced intercross which indicated that significant differences between the founder lines and resulting crosses exist for absorption of specific, environmentally important, nutrients. GBL has continued work to identify candidate genes to incorporate into QTL studies by identifying several genes involved in phosphorus absorption in the small intestine by using microarrays consisting of 3,072 unique cDNAs specific for the small intestine. IA has developed A three-generation resource population to detect quantitative trait loci (QTL) affecting egg production and egg quality traits in layers,. AR has developed a subpopulation of their F2 broiler cross developed to study abdominal pigmentation that has been typed for both their alleles at the dominant white (I) locus and the extended black (E) locus.

Please see the NC1008 homepage for the full accomplishments report.

ARKANSAS, UNIVERSITY OF ARKANSAS, Fayetteville, AR

Cisar C.R., J.M. Balog, R. Okimoto, N.B. Anthony, and A.M. Donoghue. 2003. The chicken bone morphogenetic protein receptor type II (BMPR2) gene maps to chromosome 7. Animal Genetics. 34: 465-476.

Cornett, L.E., J.D. Kirby, J.A. Vizcarra, J.C. Ellison, J. Thrash, P. R. Mayeux, M.D. Crew, S.M. Jones, N. Ali and D.A. Baeyens. 2003. Regulatory Peptides 110:231-239.

Jurkevich, A., L.R. Berghman, L.E. Cornett and W.J. Kuenzel. 2003.Immunohistochemical visualization of the vasotocin VT2 receptor in the pituitary gland of the domestic chicken. Poultry Sci. 82 (Suppl.): 81.

Jurkevich, A., L.R. Berghman, L.E. Cornett and W.J. Kuenzel. 2003.
Immunohistochemical characterization of chicken pituitary cells containing the vasotocin VT2 receptor. Soc. Neurosci. Abstr. 33: #710.10.

Kuenzel, W.J. 2003. Neurobiology of molt in avian species. Poultry Science 82:981-991.

Kuenzel, W.J., M.M. Abdel-Maksoud, T. Elsasser and J.A Proudman. 2003. Sulfamethazine advances puberty in male chicks by effecting a rapid increase in gonadotropins. Comp. Biochem. Physiol. A (in press)

Kuenzel, W.J., M.B. Ferrari, T. Rathinam and H. Li. 2003. Cerebrospinal fluid-
contacting neurons in the lateral septal organ (LSO) appear to function as encephalic photoreceptors. Soc. Neurosci. Abstr. 33: #827.9.

Li, H., M. Ferrari and W. Kuenzel. 2003. Light-induced reduction of cytoplasmic free calcium in neurons proposed to be encephalic photoreceptors (EPRs) in chick brain. Poultry Sci. 82 (Suppl. 1): 45-46.

Ling, M.K., M.C. Lagerstrom, R. Fredriksson, R. Okimoto, N.I. Mundy, S. Takeuchi, H.B. Schioth. 2003. Association of feather colour with constituatively active melanocortin 1 receptors in chicken. Eur. J. Biochem. 270: 1441-1449.

Reiner, A., D.J. Perkel, L.L. Bruce, A.B. Butler, A.Csillag, W. Kuenzel, L. Medina, G.Paxinos, T. Shimizu, G. Striedter, M. Wild, G.F. Ball, S. Durand, O. Güntürkün, D.W. Lee, C.V. Mello, A. Powers, S.A. White, G. Hough, L. Kubikova, T.V. Smulders, K. Wada, J. Dugas-Ford, S. Husband, K. Yamamoto, J. Yu, C. Siang, and E.D. Jarvis. 2004. Revised nomenclature for avian telencephalon and some related brainstem nuclei. J. Comp. Neurol. (in press).

Reiner, A., D.J. Perkel, L.L. Bruce, A.B. Butler, A. Csillag, W. Kuenzel, L.Medina, G.Paxinos,T. Shimizu, G. Striedter, M. Wild, G.F. Ball, S. Durand, O. Güntürkün, D.W. Lee, C.V. Mello, A. Powers, S.A. White, G. Hough, L. Kubikova, T.V. Smulders, K. Wada, J. Dugas-Ford, S. Husband, K. Yamamoto, J. Yu, C. Siang, and E.D. Jarvis. 2004. The Avian Brain Nomenclature Forum: Terminology for a new century in comparative neuroanatomy. J. Comp. Neurol. (in press).

Scarbrough, K., J.D. Kirby, L.E. Cornett and R. Okimoto. 2003. Chromosomal
assignment and mapping of the vasotocin receptor 1, homologue to the
mammalian V1a and Vasotocin receptor 2 homogogue to the mammalian V1b receptors in the domestic fowls. Anim. Genetics 34:384-397.

Sharma, P.M., D.W. Salter, W.S. Payne, and R. Okimoto. 2003. Chicken melanosomal matrix protein is a candidate gene for the dominant white phenotype. Plant, Animal, and Microbe, Genome XI. Abstract guide.

Sharma, P.M., W.G. Bottje, and R. Okimoto. 2003. Association of variant alleles of the uncoupling protein with feed efficiency in broiler chickens. Poultry Sci. 82 (suppl. 1) page 23.

Takeuchi, S., S. Takahashi, R. Okimoto, H. B. Schiöth, T. Boswell. 2002. Avian
melanocortin system: alpha MSH may be acting as an autocrine/paracrine
hormone. 5th International Melanocortin meeting. Sunriver Oregon. Published in Ann NY Acad Sci. 2003, 994: 366-372.

Thilakar, R. and W. Kuenzel. 2003. Effect of electrolytic lesions of the lateral septal organ on gonadal development in male broiler chicks (Gallus domesticus). Poultry Sci. 82 (Suppl. 1): 44.

Ugrankar, R.B., K. Cheng, and R. Okimoto. 2003. DNA sequence of melanocortin 1-receptor gene in Coturnix japonica: correlation with three E locus alleles, E, e+, and erh. Discovery. 4: 83-88.


CALIFORNIA, CITY OF HOPE, Duarte, CA

Goto, R.M., Afanassieff, M., Ha, J., Iglesias, G.M., Ewald, S.J., Briles, W.E., and Miller, M.M. 2002. Single strand conformational polymorphism (SSCP) assays for major histocompatibility complex B genotyping in chickens. Poultry Science 81:1832-1841.

Iglesias, G.M., Soria, L.A., Goto, R.M., Jar, A., Miquel, M.C., Lopez, O.J., and Miller, M.M. 2003. Genotypic variability at the major histocompatibility complex (B and Rfp-Y) in Camperos broiler chickens. Animal Genetics 34:88-95.

Liu, W. Miller, M.M. and Lamont, S.J. 2002. Association of MHC class I and class II gene polymorphisms with vaccine or challenge response to Salmonella enteritidis in young chicks. Immunogenetics 54: 582-590.

Thoraval, P., Afanassieff, M., Bouret, D., Luneau, G., Esnault, E., Goto, R.M., Chausse, A.-M., Zoorob, R., Soubieux, D., Miller, M.M., and Dambrine, G. 2003. Role of nonclassical class I genes of the chicken major histocompatibility complex Rfp-Y locus in transplantation immunity. Immunogenetics. 55:647-651.


CALIFORNIA, UNIVERSITY OF CALIFORNIA, DAVIS, Davis CA

Daniels, L.M. and M.E. Delany. 2003. Molecular and cytogenetic organization of the 5S ribosomal DNA in chicken (Gallus gallus). Chromosome Research 11:305-317.

Delany, M.E.. 2003. Genetic diversity and conservation of poultry. In Poultry Genetics, Breeding and Biotechnology (editors: W.E. Muir and S.E. Aggrey). Chapter 15, pp. 257-281. CABI Publishing, Wallingford, UK.

Delany, M.E. 2003. Organization and dynamics of chicken telomeres. In Proceedings of the 52nd Annual National Breeders Roundtable, St. Louis MO.

Delany, M.E., L.M. Daniels, S.E. Swanberg, and H.A. Taylor. 2003. Telomeres in the chicken: Chromosome ends and genome stability. Poultry Science 82:917-926.

Delany, M.E., and L.M. Daniels. In press. The chicken telomerase RNA gene: Conservation of sequence, regulatory elements and synteny among viral, avian and mammalian genomes. Cytogenetic and Genome Research.

Fulton, J.E. and M.E. Delany. 2003. Diminishing poultry genetic research resources: Rescue needed. Science 300:1667-1668.

Swanberg, S.E., and M.E. Delany. In press. Dynamics of telomere erosion in transformed and non-transformed avian cells in vitro. Cytogenetic and Genome Research.



DELAWARE, UNIVERSITY OF DELAWARE, Newark,DE

Bliss, T. W., M. G. Emara, and C. L. Keeler, Jr. 2003. Construction and Characterization of EST libraries From Differentially Stimulated Avian Macrophage Cells. 103rd American Society for Microbiology general meeting, Washington, DC, May 18-22.

Emara, M. G., and H. Kim. 2003. Genetic markers and their application in poultry breeding. Poultry Sci. 82:952-957.

Kim, H., and M. G. Emara. 2003. Characterization of the F-box Protein Family in Chickens. J. Heredity, submitted.

Kim, H., C. J. Schmidt, K. S. Decker, and M. G. Emara. 2003. A double-screening method to identify reliable candidate non-synonymous SNPs from chicken EST data. Animal Genetics 34:249-254.


INDIANA, PURDUE UNIVERSITY, West Lafayette, IN

Cheng, H.W., P. Singleton and W.M. Muir. 2003. Social stress differentially regulates neuroendocrine responses in laying hens: I. Genetic basis of dopamine responses under three different social conditions. Psychoneuroendocrinology 28:597-611.

Cheng, H.W., P. Singleton and W.M. Muir. 2003. Social stress in laying hens: Differential effect of stress on plasma dopamine concentrations and adrenal function in genetically selected chickens. Poult. Sci. 82:192-198.

Muir, W.M.. 2003. Indirect Selection for Improvement of Animal Well-Being. Chapter 14, p247-256. In Poultry Breeding and Biotechnology Eds. WM Muir and S Aggrey. CABI Press Cambridge MA.

Muir, W.M.. 2003. Incorporating Molecular Information in Breeding Programs, Applications and Limitations. Chapter 28 p549-562. In Poultry Breeding and Biotechnology Eds. WM Muir and S Aggrey. CABI Press Cambridge MA.


IOWA, IOWA STATE UNIVERSITY, Ames, IA

Deeb, N. and Lamont, S. J. 2003. Use of a novel outbred by inbred F1 cross to detect genetic markers for growth. Anim. Genet. 34:205-212.

Goodenbuhr, J.M., Kaiser, M. G., and Lamont, S.J. 2004. Linkage mapping of Inhibitor of apoptosis protein-1 (IAP1) to chicken chromosome 1. Anim. Genet. (in press).

Kaiser, M. G., Lakshmanan, N., Arthur, J. A., O?Sullivan, P., Kuhn, M., and Lamont, S. J. 2003. Experimental population design for estimation of dominant molecular marker effect on egg-production traits. Anim. Genet. 34:334-338.

Kaiser, M. G., Lanning, J. D., Ingleby, L. J., and Lamont, S. J. 2003. Interval mapping of QTL for salmonella colonization in F1 crosses if outbred by inbred chickens. In: Proc. Plant and Animal Genome XI, San Diego, CA, January 11-15.

Kramer, J., Malek, M., and Lamont, S. J. 2003. Association of twelve candiate gene polymorphisms and response to challenge with Salmonella enteritidis in poultry. Anim. Genet. 34 339-348.

Kramer, J., Malek, M., and Lamont, S. J. 2003. Candidate gene analysis to salmonella resistance in meat-type chickens. In: Proc. Plant and Animal Genome XI, San Diego, CA, January 11-15.

Lamont, S. J. 2003. Unique population designs used to address molecular genetics questions in poultry. Poultry Sci. 82:882-884.

Lamont, S. J., Pinard-van der Laan, M.-H., Cahaner, A., van der Poel, J. J., and Parmentier, H. K. 2003. Selection for disease resistance: direct selection on the immune response. Pp. 399-418. In: Poultry Genetics, Breeding and Biotechnology. Muir, W. M. and Aggrey, S. E., eds., CAB International.
Li, H., Deeb, N., Zhou, H., Mitchell, A.D., Ashwell, C.M., and Lamont, S.J. 2003. Chicken quantitative trait loci for growth and body composition associated with transforming growth factor-b genes. Poultry Sci. 82:347-356.

Liu, W. and Lamont, S.J. 2003. Candidate gene approach: potential association of Caspase-1, Inhibitor of Apoptosis Protein-1, and Prosaposin gene polymorphisms with response to Salmonella enteritidis challenge or vaccination in young chicks. Anim. Biotech. 14:61-76.

Liu, W., Kaiser, M.G., and Lamont, S.J. 2003. Natural Resistance-Associated Macrophage Protein 1 Gene Polymorphisms and Response to Vaccine against or Challenge with Salmonella enteritidis in Young Chicks. Poultry Sci. 82:259-266.

Lonergan, S. M., Deeb, N., Fedler, C. A., and Lamont, S. J. 2003. Breast meat quality and composition in unique chicken populations. Poultry Sci. 82:1990-1994.

Lonergan, S. M., Deeb, N., Fedler, C. A., and Lamont, S. J. 2003. Breast meat quality and composition in unique chicken populations. Poultry Sci. 82(Suppl. 1):48.

Malek, M. and Lamont, S. J. 2003. Association of INOS, TRAIL, TGFb2, TGFb3, and IgL genes with response to Salmonella enteritidis in poultry. Genet. Sel. Evol. 35 (Suppl. 1):S99-S111.

Malek, M., and Lamont, S. J. 2003. Response to Salmonella enteritidis in chickens associated with INOS, TRAIL, TGFB2, TGFB3, and IGL genes. In: Proc. Plant and Animal Genome XI, San Diego, CA, January 11-15.

Malek, M., Hasenstein, J., and Lamont, S. J. 2004. Analysis of chicken TLR4, CD28, MIF, MD-2, and LITAF genes in a Salmonella enteritidis resource population. Poultry Sci. (in press).

McElroy, J. P., Cheng, H. H., Fulton, J., Soller, M., Lipkin, E., Dekkers, J. C. M., and Lamont, S. J. 2003. Markers associated with Marek?s disease survival in commercial layers. Poultry Sci. 82(Suppl. 1):24.

Wang, J. 2003. Interval mapping of QTL with selective DNA pooling data. Ph.D. Dissertation, Iowa State University.

Wang, J., Fulton, J., and Dekkers, J. 2003. Mapping quantitative trait loci affecting economic traits in layers. Poultry Sci. (Suppl. 1):49.

Wang, J., Fulton, J., and Dekkers, J. 2003. Accuracy of detecting quantitative trait loci by selective DNA pooling. J. Anim. Sci. 81 (Suppl. 2):

Wang, J., Koehler, K., Soller, M., and J.C.M. Dekkers, J.C.M., 2003. Least squares interval mapping to detect QTL with selective DNA pooling. Plant and Animal Genome XI: 69.

Zhou, H., Deeb, N., Mitchell, A., Ashwell, C., and Lamont, S. J. 2003. Associations of BMP genes with skeletal integrity traits in chickens. Poultry. Sci. 82(Suppl. 1):23.

Zhou, H. and Lamont, S. J. 2003. Associations of six candidate genes with antibody response kinetics in hens. Poultry Sci. 82:1118-1126.

Zhou, H. and Lamont, S.J. 2003. Association of transforming growth factor b genes with quantitative trait loci for antibody response kinetics in hens. Anim. Genet. 34:275-282.

Zhou, H. and Lamont, S. J. 2003. Chicken MHC class I and II gene effects on antibody response kinetics in adult chickens. Immunogenet. 55:133-140.

Zhou, H. and Lamont, S. J. 2003. Network interactions among fifteen candidate genes for immunity hens. In: Proc. Plant and Animal Genome XI, San Diego, CA, January 11-15.

Zhou, H., Li, H. and Lamont, S. J. 2003. Genetic markers associated with antibody response kinetics in adult chickens. Poultry Sci. 82:699-708.


MARYLAND, UNIVERSITY OF MARYLAND, College Park, MD

Cogburn LA, Wang X, Carre W, Rejto L, Porter TE, Aggrey SE, Simon J (2003) Systems-wide chicken DNA microarrays, gene expression profiling, and discovery of functional genes. Poult Sci. 2003 Jun;82(6):939-51.


MICHIGAN, MICHIGAN STATE UNIVERSITY, East Lansing, MI

Dodgson, J.B. 2003. The future of molecular genetics in poultry breeding. In W. M. Muir and S.E. Aggrey (eds.), Poultry genetics, breeding and biotechnology. CABI Publishing, New York, NY, pp. 685-695.

Dodgson, J.B. 2003. Chicken genome sequence: a centennial gift to poultry genetics. Cytogenetics and Genome Research, in press.

Dodgson, J.B. and Romanov, M.N. 2004. Use of chicken models for the analysis of human disease. In N.C. Dracopoli, J.L. Haines, B.R. Korf, D.T. Moir, C.C. Morton, C.E. Seidman, J.G. Seidman, and D.R. Smith (eds.) Current Protocols in Human Genetics. John Wiley & Sons, Hoboken, NJ, in press.

Lee, M.-K., C.W. Ren, B. Yan, B. Cox, H.-B. Zhang, M.N. Romanov, F.G. Sizemore, S.P. Suchyta, E. Peters, and J.B. Dodgson 2003. Construction and characterization of three BAC libraries for analysis of the chicken genome, Animal Genetics 34:151-152.

Ren C., M.K. Lee, B. Yan, K. Ding, B. Cox, M.N. Romanov, J.A. Price, J.B. Dodgson and H.B. Zhang. 2003. A BAC-based physical map of the chicken genome. Genome Research 13:2754-2758.

Romanov M.N., J.A. Price, and J.B. Dodgson. 2003. Integration of animal linkage and BAC contig maps using overgo hybridization. Cytogenetics and Genome Research, in press.


MINNESOTA, UNIVERSITY OF MINNESOTA, St. Paul, MN

Alvarez, R., H.C.Lwamba, D.R. Kapczynski, M.K. Njenga, B.S. Seal. 2003. Nucleotide and predicted amino acid sequence-based analysis of the avian metapneumovirus type C cell attachment glycoprotein gene: Phylogenetic analysis and molecular epidemiology of U.S. Pneumoviruses. J. Clin. Microbiol. 41:1730-1735.

Chaves, L.D., B.J. Ostroski, and K.M. Reed. 2004. Myosin light chain genes in the turkey (Meleagris gallopavo). Cytogenet Genome Res. In press.

Christman S.A., B.-W. Kong, M.M. Landry, H. Kim, and D.N. Foster. 2003 Fluctuations in the p53 and Rb/p16INK4a pathways contribute to the spontaneous immortalization of a chicken embryo fibroblast cell line. Molec. Biol Cell. (suppl)14: 165a-166a.

Dranchak, P, L.D. Chaves, J.A. Rowe, and K.M. Reed. 2003. Turkey microsatellite loci from an embryonic cDNA library. Poultry Sci. 82:526-531.

Harry, D.E., P.J. Marini, D. Zaitlin, and K.M. Reed. 2003. A first generation map of the turkey genome. Genome. 46:914-924.

Kong, B-W., H. Kim, and D. N. Foster. 2003. Expression analysis and mitochondrial targeting properties of the chicken manganese-containing superoxide dismutase. Biochem. Biophys. Acta. 1625:98-108.

Kong, B-W., H. Kim, and D. N. Foster. 2003. Cloning and expression analysis of chicken phospholipid-hydroperoxide glutathione peroxidase. Animal Biotechnology. 14:19-29.

Lwamba H.C., D.A. Halvorson, K.V. Nagaraja, E.A. Turpin, D. Swayne, B.S. Seal, M.K. Njenga. 2002. Antigenic cross-reactivity among avian pneumoviruses of subgroups A, B, and C at the matrix but not nucleocapsid proteins. Avian Dis 46:725-729.

Lwamba H.C., R.S. Bennett, D.C. Lauer, D.A. Halvorson, M.K. Njenga. 2002. Characterization of avian metapneumoviruses isolated in the USA. Anim. Health Res Rev. 3:107-117.

Munir, S. and V. Kapur. 2003. Regulation of hohst cell transcriptional physiology by the avian pneumovirus provides key insights into host-pathogen interactions. J. Virol. 4899-4910.

Njenga, M. K., H.C.M. Lwamba, B.S. Seal. 2003. Metapneumoviruses in birds and humans. Virus Res. 91:163-169.

Reed, K.M., L.D. Chaves, M.K. Hall, T.P. Knutson, J.A. Rowe, and A.J. Torgerson. 2003. Microsatellite loci for genetic mapping in the turkey (Meleagris gallopavo). Animal Biotech. 14:119-131.

Reed K.M., L.D. Chaves, J.J. Garbe, Y. Da, and D.E. Harry. 2004. Allelic variation and genetic linkage of avian microsatellites in a new turkey population for genetic mapping. Cytogenet Genome Res. In press.


NORTH CAROLINA, NORTH CAROLINA STATE UNIVERSITY, Raleigh, NC

Giamario C., J.N. Petitte and P.E. Mozdziak. 2003. Hatchability of chicken embryos following somite manipulation. Biotechniques 34:1128-1130.

Mozdziak, P.E., S. Borwornpinyo, D.W. McCoy and J.N. Petitte. 2003. The development of transgenic chickens expression bacterial beta-galactosidase. Developmental Dynamics 226:439-445.

Mozdziak, P.E., C. Giamario and J.N. Petitte. 2003. Myonuclear accretion a brief review. Animal Science Papers and Reports 21 (supplement 1):121-131.

Mozdziak, P.E., S. Pophal, S. Borwornpinyo, and J.N. Petitte. 2003. Transgenic chickens expressing beta-galactosidase hydrolyze lactose in the intestine. J. of Nutrition 133:3076-3079.

Petitte, J.N. 2003. Strategies for the production of transgenic poultry. In W. M. Muir and S.E. Aggrey (eds.), Poultry genetics, breeding and biotechnology. CABI Publishing, New York, NY, Chapter 33.


USDA, USDA-ARS; AVIAN DISEASE ONCOLOGY LABORATORY, East Lansing, MI

Cheng, H.H. Molecular genetic techniques for identifying disease resistance genes. 2003. In Poultry Breeding and Biotechnology. W. Muir and S. Aggrey, eds. (CABI Publishing), pp. 385-398.

Liu, H.C., and Cheng, H.H.. 2003. Genetic mapping of the chicken stem cell antigen 2 (SCA2) gene to chromosome 2 via PCR primer mutagenesis. Animal Genetics 34:158-160.

Liu, H.C., Niikura, M., Fulton, J., and Cheng, H.H. Identification of chicken stem lymphocyte antigen 6 complex, locus E (LY6E, alias SCA2) as a putative Marek?s disease resistance gene via a virus-host protein interaction screen. Cytogen. Genome Res., in press.

Niikura, M., Liu, H.-C., Dodgson, J.B., and Cheng, H.H. A comprehensive screen for chicken proteins that interact with proteins unique to virulent strains of Marek?s disease virus. Submitted.

Okumura, F., Shimogiri, T., Shinbo, Y., Yoshizawa, K., Kawabe, K., Mannen, H., Okamoto, S., Cheng, H.H., and Maeda, Y. Linkage mapping of four chicken calpain genes. Submitted.

Zhu, J.J., Lillehoj, H.S., Allen, P.C., Van Tassell, C.P., Sonstegard, T.S., Cheng, H.H., Pollock, D., Sadjadi, M., Min, W., and Emara, M.G. 2003. Mapping quantitative trait loci associated with resistance to coccidiosis and growth. Poultry Science 82:9-16.


USDA, USDA-ARS; GROWTH BIOLOGY LABORATORY, Beltsville, MD

Li, H., Deeb, N., Zhou, H.t Mitchell, AD., Ashwell, C.M., and Lamont, S.J. 2003.
Chicken quantitative trait loci for growth and body composition associated with transforming growth factor-? genes. Poultry Sci. 82:347-356.

Zhou, H., Deeb, N.,Mitchell,A.,Ashwell,C.,and Lamont, S. J. 2003. Associations of BMP genes with skeletal integrity traits in chickens. Poultry. Sci. 82(Suppl.1):23.