SAES-422 Multistate Research Activity Accomplishments Report
Sections
Status: Approved
Basic Information
- Project No. and Title: NC_OLD7 : Conservation, Management, Enhancement and Utilization of Plant Genetic Resources
- Period Covered: 07/01/2001 to 06/01/2002
- Date of Report: 09/27/2002
- Annual Meeting Dates: 07/23/2002 to 07/24/2002
Participants
Johnson, Burton (Burton.johnson@ndsu.nodak.edu) - ND / NDSU; Baltensperger, David (dbaltensperger1@unl.edu) - NE / U of NE; Rife, Rife (crife@oz.oznet.ksu.edu) - KS / KSU; Francis, David (francis.77@osu.edu) - OH / OSU; Widrlechner, Mark (isumw@iastate.edu) - NCRPIS USDA; Iezzoni, Amy (iezzoni@msu.edu) - MI / MSU; Block, Charles (ccblock@iastate.edu) - NCRPIS USDA; Millard, Mark (mjmillar@iastate.edu) - NCRPIS ISU; Hokansan, Stan (hokan017@umn.edu) - MN / U of MN; Luhman, Rick (rlluhman@iastate.edu) - NCRPIS ISU; Burke, Lisa (lburke@iastate.edu) - NCRPIS USDA; Reitsma, Kathy (kreitsma@iastate.edu) - NCRPIS ISU; VanRoekel, John (Bill) (beevr@iastate.edu) - NCRPIS USDA; Brummer, Charlie (brummer@iastate.edu) - IA / ISU; Boe, Arvid (arvid_boe@sdstate.edu) - SD / SDSU; Isbell, Terry (isbellta@ncaur.usda.gov) - USDA NCAUR; Bretting, Peter (pkb@ars.usda.gov) - USDA NPS; Brenner, David (dbrenner@iastate.edu) NCRPIS ISU; Hymowitz, Theodore (soyui@uiuc.edu) - IL / U of IL; Gardner, Candice (gardnerc@iastate.edu) NCRPIS USDA; Blaylock, June (djb@ars.usda.gov) - USDA OTT; Janick, Jules (janick@hort.purdue.edu) - IN / Purdue;
The 2002 NC-7 RTAC was chaired by Burton Johnson of North Dakota State University. Charlie Rife, Kansas State University, served as secretary. ISU Assoc. Dean Gerald Miller represented ISU Dean of Agriculture Catherine Wotecki, and informed the group that NC-7 project renewal was approved for period 09-01-2002 through 08-31-2007. Cathy Good provided an overview of NIMSS, the National Information Management and Support System. The project coordinates with the administrative advisor to insure that information is kept up to date. We were informed that IFAFS authorized new support at the level of $130 million (M) in 2003, $140 M in 2004, $160 M in 2005, and $200 M in 2006. Terry Isbell from USDA-NCAUR, Peopria, IL, presented a research view of the utilization lab and its collaborative research on properties of oils, proteins, and starch. Crops covered included Lesquerella, Lepidium campestris (pepper grass), Cuphea, meadow foam, and work with glucosinolates as anti-oxidants. Nomination and committee designees included David Francis, Arvid Boe, and Burton Johnson. Resolution committee designees included Theodore Hymowitz, Jules Janick, and David Baltensperger.
Peter Bretting provided information on the status of the FAOS International Treaty on Plant Genetic Resources for Food and Agriculture, and sought RTAC member comments and feedback. After some discussion, RTAC members voted to support the Secretary of Agricultures use of maximum negotiating ability in the treaty process, thereby recommending the USA become a party to the treaty. Dr. Bretting also led a discussion of how plant germplasm from PI stations has impacted crop improvement, and how this could be documented. The RTAC approved a resolution that the USDA review the broad impact of the US germplasm program on crop improvement, food security, and agricultural research.
NCRPIS curatorial reports were given by Mark Widrlechner, horticulture; Kathy Retisma, vegetables; Rick Luhman, brassica and network administration; Mark Millard, maize; Dave Brenner, amaranth, celosia, legumes, perilla, quinoa, spinach and umbels; C. Gardner for Mary Brothers, sunflowers; Charles Block, plant pathology.
C. Gardner summarized staffing and facilities changes, and needed positions to support NCRPIS objectives. The priority order for new positions included a network IT manager, a medicinal/nutriceutical plant curator, and a pollinator biologist. The new NIH grant to ISU and UI contains funds for a support person to develop Echinacea and St. Johns-wort collections at NCRPIS. RTAC members unanimously supported development of a federal IT management position. Sources of funding for a pollinator biologist were not identified.
The 2003 NC-7 RTAC meeting will be held in Wooster, OH in August; David Francis will serve as Secretary, and Charlie Rife will serve as Chair.
Peter Bretting provided information on the status of the FAOS International Treaty on Plant Genetic Resources for Food and Agriculture, and sought RTAC member comments and feedback. After some discussion, RTAC members voted to support the Secretary of Agricultures use of maximum negotiating ability in the treaty process, thereby recommending the USA become a party to the treaty. Dr. Bretting also led a discussion of how plant germplasm from PI stations has impacted crop improvement, and how this could be documented. The RTAC approved a resolution that the USDA review the broad impact of the US germplasm program on crop improvement, food security, and agricultural research.
NCRPIS curatorial reports were given by Mark Widrlechner, horticulture; Kathy Retisma, vegetables; Rick Luhman, brassica and network administration; Mark Millard, maize; Dave Brenner, amaranth, celosia, legumes, perilla, quinoa, spinach and umbels; C. Gardner for Mary Brothers, sunflowers; Charles Block, plant pathology.
C. Gardner summarized staffing and facilities changes, and needed positions to support NCRPIS objectives. The priority order for new positions included a network IT manager, a medicinal/nutriceutical plant curator, and a pollinator biologist. The new NIH grant to ISU and UI contains funds for a support person to develop Echinacea and St. Johns-wort collections at NCRPIS. RTAC members unanimously supported development of a federal IT management position. Sources of funding for a pollinator biologist were not identified.
The 2003 NC-7 RTAC meeting will be held in Wooster, OH in August; David Francis will serve as Secretary, and Charlie Rife will serve as Chair.
Accomplishments
The past years accomplishments by the NCRPIS can be summed up in three areas 1) germplasm acquisition, management, evaluation and characterization, 2) development of infrastructure to support germplasm management, and 3) development of collaborations to support current and future research.
1. In the area of germplasm management, viability testing capacity was greatly enhanced by the addition of new germination chambers and a new 0.5 FTE technician. The backlog of viability testing for maize was eliminated, and a total of 6,420, or 14% of our accessions. A testing goal of 10% of the collection holdings annually is necessary to maintain a 10 year viability testing cycle. Approximately 1,400 accessions were cultivated for regeneration, with a predicted success rate of over 90%. The number of accessions backed up at the National Center for Germplasm Resource Preservation (NCGRP) in Ft. Collins increased to 74% in 2002. Over 12,000 data points of evaluation and characterization data were loaded into GRIN, the Germplasm Resource Information Network, and made available to the public. Over 4,500 digital images were recorded of plants and seeds.
2. An intensive effort to automate the process of capturing, manipulating, managing image files and transferring them to GRIN is underway. File naming protocols were establish, and protocols partially developed to enable student employees to perform needed actions on image files. Completion of this process is anticipated in 2004, and is contingent on securing appropriate software development resources. Once complete, a second software project is planned to capture biometric information from images. A network firewall system was designed to secure our IT systems from attack, and submitted for procurement. HOBO and Palm Pilot technology was acquired for constant monitoring of our new dryers; this technology is completely portable and interfaces with a PC to provide a graphic display of environmental conditions to which it has been exposed. These will also be useful for monitoring greenhouse environmental qualities.
3. The incorporation of the GEM (Germplasm Enhancement of Maize) unit under the PI umbrella has provided opportunity to develop non-destructive technology to evaluate quality traits in crops other than maize. Efforts are underway to develop moisture calibrations using NIR (Near Infra-red Spectroscopy) for sunflowers and Cuphea, and for protein and starch composition in amaranth.
Our horticulturist, Mark Widrlechner, published results of collaborative research with researchers from Iowa State University, Michigan State University, North Carolina State, and the Ukraine on woody ornamental research, the role of environmental analogs in adaptation, and geographic assessments of the risks of naturalization by non-native woody plants in the upper midwest. Our amaranth curator, David Brenner, published collaborative research with David Baltensperger of the Univ. of Nebraska on amaranth crop development. Our vegetable curator, Kathy Reitsma, published collaborative research designed to test core collections in cucumber using a variety of data sources. All of these efforts contribute to our understanding of how germplasm can be used for varietal development and improvement, and provide unique, useful traits.
Accomplishments of non-NCRPIS, NC-7 investigators are extensive, broad, and provide significant impact. Although uncertain how to apportion these land-grant researchers efforts to NC-7 or other project resources, the following are included and cited (this list is not inclusive of all RTAC member institutions):
Jules Janick of Purdue University, IN, has contributed extensively to research on Arugula, a leafy vegetable, by acquiring germplasm and by evaluating its economic potential.
Amy Iezzoni, of Michigan State University, has made publicly available the fingerprinting results for 59 cherry accessions in the NPGS collection. These will be a valuable resource for germplasm curators interested in verifying accession identity and identifying novel types within their own collections.
Theodore Hymowitz, of the University of Illinois, has completed a molecular phytogenetic study of 15 genera of the subtribe Glycininae; germplasm used in the study was donated by 11 institutions. The results illuminate the relationships between Teramnus, Glycine, Amphicarpaea, and Pueraria.
Budgetary resources: ARS funding for FY02 was $1,791,266, and NC-7 provided resources of $532,522. The NC-7 funds support 12 ISU employees that include Curators, Agricultural Specialists, our Farm Superintendent and his support staff, and some operating costs. Federal funds supported 20 permanent USDA staff in FY02, including three scientists, technicians, support and administrative staff, approximately 26 student labor FTEs, and the bulk of operational expenses. A minimum of $400,000 is required annually to support operational activities at the station. Federal salary costs erode operational funding at a historical rate of approximately 8% per year; ARS funding is adequate for the next five years, given existing budgets and staffing; additional positions will require external funds. NC-7 funds available for operating costs are trending downwards due to flat budgets and modest salary growth. ARS funding increases since 1999 have made a tremendous difference in our ability to implement new technology, to improve outputs and their quality. In 1993, ARS funds provided $1,272,300 to the NCRPIS while NC-7 funds provided $448,600. In 1999, resources were essentially unchanged and staffing cuts were projected in 2000 without additional resources. Due to the successful efforts of stakeholders who convinced Congress that additional resources were needed to support the National Plant Germplasm System, the resource picture is substantially improved.
1. In the area of germplasm management, viability testing capacity was greatly enhanced by the addition of new germination chambers and a new 0.5 FTE technician. The backlog of viability testing for maize was eliminated, and a total of 6,420, or 14% of our accessions. A testing goal of 10% of the collection holdings annually is necessary to maintain a 10 year viability testing cycle. Approximately 1,400 accessions were cultivated for regeneration, with a predicted success rate of over 90%. The number of accessions backed up at the National Center for Germplasm Resource Preservation (NCGRP) in Ft. Collins increased to 74% in 2002. Over 12,000 data points of evaluation and characterization data were loaded into GRIN, the Germplasm Resource Information Network, and made available to the public. Over 4,500 digital images were recorded of plants and seeds.
2. An intensive effort to automate the process of capturing, manipulating, managing image files and transferring them to GRIN is underway. File naming protocols were establish, and protocols partially developed to enable student employees to perform needed actions on image files. Completion of this process is anticipated in 2004, and is contingent on securing appropriate software development resources. Once complete, a second software project is planned to capture biometric information from images. A network firewall system was designed to secure our IT systems from attack, and submitted for procurement. HOBO and Palm Pilot technology was acquired for constant monitoring of our new dryers; this technology is completely portable and interfaces with a PC to provide a graphic display of environmental conditions to which it has been exposed. These will also be useful for monitoring greenhouse environmental qualities.
3. The incorporation of the GEM (Germplasm Enhancement of Maize) unit under the PI umbrella has provided opportunity to develop non-destructive technology to evaluate quality traits in crops other than maize. Efforts are underway to develop moisture calibrations using NIR (Near Infra-red Spectroscopy) for sunflowers and Cuphea, and for protein and starch composition in amaranth.
Our horticulturist, Mark Widrlechner, published results of collaborative research with researchers from Iowa State University, Michigan State University, North Carolina State, and the Ukraine on woody ornamental research, the role of environmental analogs in adaptation, and geographic assessments of the risks of naturalization by non-native woody plants in the upper midwest. Our amaranth curator, David Brenner, published collaborative research with David Baltensperger of the Univ. of Nebraska on amaranth crop development. Our vegetable curator, Kathy Reitsma, published collaborative research designed to test core collections in cucumber using a variety of data sources. All of these efforts contribute to our understanding of how germplasm can be used for varietal development and improvement, and provide unique, useful traits.
Accomplishments of non-NCRPIS, NC-7 investigators are extensive, broad, and provide significant impact. Although uncertain how to apportion these land-grant researchers efforts to NC-7 or other project resources, the following are included and cited (this list is not inclusive of all RTAC member institutions):
Jules Janick of Purdue University, IN, has contributed extensively to research on Arugula, a leafy vegetable, by acquiring germplasm and by evaluating its economic potential.
Amy Iezzoni, of Michigan State University, has made publicly available the fingerprinting results for 59 cherry accessions in the NPGS collection. These will be a valuable resource for germplasm curators interested in verifying accession identity and identifying novel types within their own collections.
Theodore Hymowitz, of the University of Illinois, has completed a molecular phytogenetic study of 15 genera of the subtribe Glycininae; germplasm used in the study was donated by 11 institutions. The results illuminate the relationships between Teramnus, Glycine, Amphicarpaea, and Pueraria.
Budgetary resources: ARS funding for FY02 was $1,791,266, and NC-7 provided resources of $532,522. The NC-7 funds support 12 ISU employees that include Curators, Agricultural Specialists, our Farm Superintendent and his support staff, and some operating costs. Federal funds supported 20 permanent USDA staff in FY02, including three scientists, technicians, support and administrative staff, approximately 26 student labor FTEs, and the bulk of operational expenses. A minimum of $400,000 is required annually to support operational activities at the station. Federal salary costs erode operational funding at a historical rate of approximately 8% per year; ARS funding is adequate for the next five years, given existing budgets and staffing; additional positions will require external funds. NC-7 funds available for operating costs are trending downwards due to flat budgets and modest salary growth. ARS funding increases since 1999 have made a tremendous difference in our ability to implement new technology, to improve outputs and their quality. In 1993, ARS funds provided $1,272,300 to the NCRPIS while NC-7 funds provided $448,600. In 1999, resources were essentially unchanged and staffing cuts were projected in 2000 without additional resources. Due to the successful efforts of stakeholders who convinced Congress that additional resources were needed to support the National Plant Germplasm System, the resource picture is substantially improved.
Impacts
- NCRPIS and NC-7 researchers efforts contribute to the National Plant Germplasm System, a national acquisition and management program of plant germplasm valued for agricultural, horticultural, environmental, medicinal and industrial uses in the NCR and through the U.S.
- Plant germplasm resources numbering over 47,100 accessions of over 1,700 plant species continue to be conserved and made available to the research public. Over 70% of the collections are currently available and 74% backed up in long-term storage.
- By providing well-characterized, well-documented germplasm to researchers for a wide array of purposes, we encourage and support the use of a broad diversity of germplasm to reduce crop genetic vulnerability. The value of germplasm is greatly enhanced by high-quality associated information, which enables researchers to better target their efforts and use their resources more effectively, hence contributing to a secure, sustainable economy.
- The work of NCRPIS and NC-7 scientists to ascertain plant-environment interactions, genetic character, and the nature of plant genetic diversity contributes to our understanding of characteristics which are conducive to adaptation.
- By participating in scientific and educational meetings, hosting a wide array of visitors seeking education on plant genetic resources, and publishing our work in a peer-reviewed publications, we strive to educate students, scientists and the general public regarding plant germplasm resource issues.
Publications
Abel, C.A., Pollak, L.M., Salhuana, W., Widrlechner, M.P., Wilson, R.L. Registration of GEMS-0001 maize germplasm resistant to leaf blade, leaf sheath, and collar feeding by European Corn Borer. Crop Science. 2002. 41: 1651-1652.
D.M. Brenner, D.D. Baltensperger, C.C. Block, R.L. Myers, and I.Simon. Amaranth: Progress and problems. Abstract and oral presentation, p. 31. In Nov. 10-13, 2001. Abstracts, 5th National Symposium, New crops and new uses: Strength in diversity. Atlanta, GA.
D.M. Brenner. Regenerating Germplasm: Some methods used with Amaranthus and other Crops at the North Central Regional Plant Introduction Station. November 14, 2001. Oral presentation, Plant Genetic Resources Conservation Unit, Griffin, GA.
Graves, W.R., Kroggel, M.A., Widrlechner, M.P. Photosynthesis and shoot health of five birch and four alder taxa after drought and flooding. Journal of Environmental Horticulture. 2002. 20: 36-40.
Norris, W.R., Widrlechner, M.P., Lewis, D.Q., Thompson, J.D., Pope, R.O. More than a century of change in the Ames, Iowa flora (1859-2000). Journal of the Iowa Academy of Science. 2001. 108: 124-141.
Reitsma, K. R. and M. P. Widrlechner. 2002. Daucus in the USDA Germplasm Collection. Carrot Country 9 (3): in press.
Staub, J. E., F. Dane, K. Reitsma, F. Fazio, and A. Lopez-SesJ. 2002. The Formation of Test Arrays and a Core Collection in Cucumber Using Phenotypic and Molecular Data. J. Amer. Soc. Hort. Sci. 127(4): 558-567.
Widrlechner, M.P. The role of environmental analogs in identifying potentially invasive woody plants in Iowa. Journal of the Iowa Academy of Science. 2001. 108: 158-165.
Widrlechner, M.P., McKeown, K.A. Assembling and characterizing a comprehensive Echinacea germplasm collection. Poster Abstract N-38, Abstracts of the 5th National Symposium on New Crops and New Uses: Strength in Diversity, Atlanta, GA, 10-13 November 2001, p. 129.
Widrlechner, M.P., Schutzki, R.E., Yukhnovsky, V.Y., Sviatetsky, V.V. Collecting landscape trees and shrubs in Ukraine for the evaluation of aesthetic quality and adaptation in the north central United States. FAO/IPGRI Plant Genetic Resources Newsletter. 2001. 126: 12-16.
Widrlechner, M.P., Iles, J.K. A geographic assessment of the risk of naturalization of non-native woody plants in Iowa. Journal of Environmental Horticulture. 2002. 20: 47-56.
Lee, J. and T. Hymowitz. 2001. A molecular phylogenetic study of the subtribe Glycininae (Leguminosae) derived from the chloroplast DNA rps16 intron sequences. American J. of Botany 88:2064-2073.
Morales, M. and J. Janick. 2002. Arugula: A promising specialty leaf vegetable. P. 418-423. In: J. Janick and A. Whipkey (eds.) Trends in new crops and new uses. ASHS Press, Alexandria, VA.
Owens, C.L., J.F. Hancock. M. Thomashow, and A.F. Iezzone. 2002. CBF-1 orthologs in sour cherry and strawberry and the heterologous expression of CBF1 in strawberry. J. Amer. Soc. Hort. Sci. 127:489-494.
Hauck, A., A. Iezzoni, H. Yamane, and R. Tao. 2002. Self-compatibility and incompatibility in tetraploid sour cherry (Prunus cerasus L.) Sexual Plant Reproduction 15:39-46.
Struss, D., M. Boritzki, R. Karle, and A.F. Iezzoni. 2002. Microsatellite markers differentiate eight Giessen cherry rootstocks. HortScience 37:191-193.
Yamane, H. R. Tao, A. Sugiura, N.R. Hauck, and A.F. Iezzoni. 2001. Idnetification and characterization of S-RNases in tetraploid sour cherry (Prusus cerasus). Jour. Amer. Soc. Hort. Sci. 126:661-667.
Huck, N.R., A.F. Iezzoni, H. Yamane, and R. Tao. 2001. Revisiting the S-allele nomenclature in sweet cherry (Prunus avium) using RFLP profiles. Jour. Amer. Soc. Hort. Sci. 126:654-660.
Cantini, C., A.F. Iezzoni, W.F. Lamboy, M. Bortizki, and D. Struss. 2001. DNA fingerprinting of tetraploid cherry germplasm using simple sequence repeats. J. Amer. Soc. Hort. Sci. 126:205-209.
Eghball, B., J. E. Gilley, D. D. Baltensperger, and J. M. Blumenthal. 2002. Long-Term Manure and Fertilizer Application Effects on Phosphorus and Nitrogen in Runoff. Trans. of the ASAE 45(3):687-694. Univ. of Nebraska Agr. Res. Div. Paper No. 13289.
Rife, C.L., D.L. Auld, H.D. Sunderman, W.F. Heer, D.D. Baltensperger, L.A. Nelson, D.L. Johnson, D. Bordovsky, and H.C. Minor. 2001. Registration of ‘Wichita‘ Rapeseed. Crop Sci. 41(1):263-264.
Rife, C., G. Warman, and W. Heer. 2001. 2000 Great Plains Canola Research. Report of Progress 862. Kansas Ag. Exp. St. Manhattan. (AES# 01-184-S)
Rife, C. 2002. Winter Canola can help spread risk: Results from the 2000-2001 National Winter Canola Variety Trial. Canola Grower. 4:1:12-17. (AES# 02-226-T)
Rife, C.L. 2002. United States National Winter Canola Variety Trials. In Book of Abstracts. 13th Crucifer Genetics Workshop. Davis, CA. Page 118. Rife C.L. and H. Zeinali.. 2003. Cold Tolerance in Oilseed Rape over Varying Acclimation Durations. Crop Sci. 43:96-100. (AES# 02-262-J)
Rife, C.L. and C. Labarge. 2002. Variability in Vernalization Requirement of Oilseed Rape. In Annu. Meet. Abs. Amer. Soc. Agron.
Rife C., R. Aiken, R. Auld, R. Bacon, D. Baltensperger, U. Bishnoi, H. Bhardwaj, D. Bordovsky, E. Christmas, S. Conley, P. Evans, J. Fisher, R. Freed, W. Heer, K. Janssen, J. Johnson, J. Johnson, J. Kelly, J. Klein, R. Kochenower, J. Krall, K. Kusel, K. Larson, J. Long, L. Nelson, P. Raymer, M. Schmidt, F. Schweissing, R. Smoot D. Starner, M. Witt, 2003. 2002 National Winter Canola Variety Trial. Departmental Report. Kansas Ag. Exp. St. Manhattan. 58 pp. (AES# 03-214-D)
Rife, C. 2002. Winter Canola can help spread risk: Results from the 2000-2001 National Winter Canola Variety Trial. Canola Grower. 4:1:12-17. (AES# 02-226-T)
Rife, C.L. 2002. United States National Winter Canola Variety Trials. In Book of Abstracts. 13th Crucifer Genetics Workshop. Davis, CA. Page 118.
Rife, C.L. and C. Labarge. 2002. Variability in Vernalization Requirement of Oilseed Rape. In Annu. Meet. Abs. Amer. Soc. Agron
D.M. Brenner, D.D. Baltensperger, C.C. Block, R.L. Myers, and I.Simon. Amaranth: Progress and problems. Abstract and oral presentation, p. 31. In Nov. 10-13, 2001. Abstracts, 5th National Symposium, New crops and new uses: Strength in diversity. Atlanta, GA.
D.M. Brenner. Regenerating Germplasm: Some methods used with Amaranthus and other Crops at the North Central Regional Plant Introduction Station. November 14, 2001. Oral presentation, Plant Genetic Resources Conservation Unit, Griffin, GA.
Graves, W.R., Kroggel, M.A., Widrlechner, M.P. Photosynthesis and shoot health of five birch and four alder taxa after drought and flooding. Journal of Environmental Horticulture. 2002. 20: 36-40.
Norris, W.R., Widrlechner, M.P., Lewis, D.Q., Thompson, J.D., Pope, R.O. More than a century of change in the Ames, Iowa flora (1859-2000). Journal of the Iowa Academy of Science. 2001. 108: 124-141.
Reitsma, K. R. and M. P. Widrlechner. 2002. Daucus in the USDA Germplasm Collection. Carrot Country 9 (3): in press.
Staub, J. E., F. Dane, K. Reitsma, F. Fazio, and A. Lopez-SesJ. 2002. The Formation of Test Arrays and a Core Collection in Cucumber Using Phenotypic and Molecular Data. J. Amer. Soc. Hort. Sci. 127(4): 558-567.
Widrlechner, M.P. The role of environmental analogs in identifying potentially invasive woody plants in Iowa. Journal of the Iowa Academy of Science. 2001. 108: 158-165.
Widrlechner, M.P., McKeown, K.A. Assembling and characterizing a comprehensive Echinacea germplasm collection. Poster Abstract N-38, Abstracts of the 5th National Symposium on New Crops and New Uses: Strength in Diversity, Atlanta, GA, 10-13 November 2001, p. 129.
Widrlechner, M.P., Schutzki, R.E., Yukhnovsky, V.Y., Sviatetsky, V.V. Collecting landscape trees and shrubs in Ukraine for the evaluation of aesthetic quality and adaptation in the north central United States. FAO/IPGRI Plant Genetic Resources Newsletter. 2001. 126: 12-16.
Widrlechner, M.P., Iles, J.K. A geographic assessment of the risk of naturalization of non-native woody plants in Iowa. Journal of Environmental Horticulture. 2002. 20: 47-56.
Lee, J. and T. Hymowitz. 2001. A molecular phylogenetic study of the subtribe Glycininae (Leguminosae) derived from the chloroplast DNA rps16 intron sequences. American J. of Botany 88:2064-2073.
Morales, M. and J. Janick. 2002. Arugula: A promising specialty leaf vegetable. P. 418-423. In: J. Janick and A. Whipkey (eds.) Trends in new crops and new uses. ASHS Press, Alexandria, VA.
Owens, C.L., J.F. Hancock. M. Thomashow, and A.F. Iezzone. 2002. CBF-1 orthologs in sour cherry and strawberry and the heterologous expression of CBF1 in strawberry. J. Amer. Soc. Hort. Sci. 127:489-494.
Hauck, A., A. Iezzoni, H. Yamane, and R. Tao. 2002. Self-compatibility and incompatibility in tetraploid sour cherry (Prunus cerasus L.) Sexual Plant Reproduction 15:39-46.
Struss, D., M. Boritzki, R. Karle, and A.F. Iezzoni. 2002. Microsatellite markers differentiate eight Giessen cherry rootstocks. HortScience 37:191-193.
Yamane, H. R. Tao, A. Sugiura, N.R. Hauck, and A.F. Iezzoni. 2001. Idnetification and characterization of S-RNases in tetraploid sour cherry (Prusus cerasus). Jour. Amer. Soc. Hort. Sci. 126:661-667.
Huck, N.R., A.F. Iezzoni, H. Yamane, and R. Tao. 2001. Revisiting the S-allele nomenclature in sweet cherry (Prunus avium) using RFLP profiles. Jour. Amer. Soc. Hort. Sci. 126:654-660.
Cantini, C., A.F. Iezzoni, W.F. Lamboy, M. Bortizki, and D. Struss. 2001. DNA fingerprinting of tetraploid cherry germplasm using simple sequence repeats. J. Amer. Soc. Hort. Sci. 126:205-209.
Eghball, B., J. E. Gilley, D. D. Baltensperger, and J. M. Blumenthal. 2002. Long-Term Manure and Fertilizer Application Effects on Phosphorus and Nitrogen in Runoff. Trans. of the ASAE 45(3):687-694. Univ. of Nebraska Agr. Res. Div. Paper No. 13289.
Rife, C.L., D.L. Auld, H.D. Sunderman, W.F. Heer, D.D. Baltensperger, L.A. Nelson, D.L. Johnson, D. Bordovsky, and H.C. Minor. 2001. Registration of ‘Wichita‘ Rapeseed. Crop Sci. 41(1):263-264.
Rife, C., G. Warman, and W. Heer. 2001. 2000 Great Plains Canola Research. Report of Progress 862. Kansas Ag. Exp. St. Manhattan. (AES# 01-184-S)
Rife, C. 2002. Winter Canola can help spread risk: Results from the 2000-2001 National Winter Canola Variety Trial. Canola Grower. 4:1:12-17. (AES# 02-226-T)
Rife, C.L. 2002. United States National Winter Canola Variety Trials. In Book of Abstracts. 13th Crucifer Genetics Workshop. Davis, CA. Page 118. Rife C.L. and H. Zeinali.. 2003. Cold Tolerance in Oilseed Rape over Varying Acclimation Durations. Crop Sci. 43:96-100. (AES# 02-262-J)
Rife, C.L. and C. Labarge. 2002. Variability in Vernalization Requirement of Oilseed Rape. In Annu. Meet. Abs. Amer. Soc. Agron.
Rife C., R. Aiken, R. Auld, R. Bacon, D. Baltensperger, U. Bishnoi, H. Bhardwaj, D. Bordovsky, E. Christmas, S. Conley, P. Evans, J. Fisher, R. Freed, W. Heer, K. Janssen, J. Johnson, J. Johnson, J. Kelly, J. Klein, R. Kochenower, J. Krall, K. Kusel, K. Larson, J. Long, L. Nelson, P. Raymer, M. Schmidt, F. Schweissing, R. Smoot D. Starner, M. Witt, 2003. 2002 National Winter Canola Variety Trial. Departmental Report. Kansas Ag. Exp. St. Manhattan. 58 pp. (AES# 03-214-D)
Rife, C. 2002. Winter Canola can help spread risk: Results from the 2000-2001 National Winter Canola Variety Trial. Canola Grower. 4:1:12-17. (AES# 02-226-T)
Rife, C.L. 2002. United States National Winter Canola Variety Trials. In Book of Abstracts. 13th Crucifer Genetics Workshop. Davis, CA. Page 118.
Rife, C.L. and C. Labarge. 2002. Variability in Vernalization Requirement of Oilseed Rape. In Annu. Meet. Abs. Amer. Soc. Agron