SAES-422 Multistate Research Activity Accomplishments Report
Sections
Status: Approved
Basic Information
- Project No. and Title: WERA_OLD20 : Virus and Virus-Like Diseases of Fruit Trees, Small Fruits, and Grapevines.
- Period Covered: 10/01/2014 to 09/01/2015
- Date of Report: 11/25/2015
- Annual Meeting Dates: 07/06/2015 to 07/10/2015
Participants
Al Rwahnih, Maher (malrwahnih@ucdavis.edu) - Univ of California-Davis<br> Almeyda, Christie - NC State University<br> Bateman, Margarita (margarita.f.licha@aphis.usda.gov) - USDA-APHIS-PPQ<br> Cao, Mengji - USDA-ARS<br> Cheong, Eun Ju - USDA-ARS<br> Eastwell, Ken (keastwell@wsu.edu) - Washington State Univ<br> Foster, Joseph - USDA-APHIS-PPQ<br> Fuchs, Mark (marc.fuchs@cornell.edu) - Cornell Univ.<br> Golino, Deborah (dagolino@ucdavis.edu) - Univ. of California-Davis, Foundation Plant Services<br> Grinstead, Sam - USDA-ARS<br> Guerra, Lauri (lguerra@agr.wa.gov) - WA State Dept Agriculture<br> Howell, Bill - Northwest Nursery Institute<br> Hu, John (johnhu@hawaii.edu) - University of Hawaii<br> Jones, Robert - USDA-APHIS-PPQ<br> Jordan, Ramon - USDA-ARS<br> Karasev, Alexander (akarasev@uidaho.edu) - University of Idaho<br> Kinard, Gary - USDA-ARS<br> Li, Ruhui (Ruhui.Li@ars.usda.gov) - USDA-ARS<br> Liu, Zhaowei - USDA-APHIS-CPHST<br> Martin, Bob (bob.martin@ars.usda.gov) - USDA-ARS<br> Mavrodieva, Vessela - USDA-APHIS-PPQ<br> Melzer, Michael (melzer@hawaii.edu) - University of Hawaii<br> Mollov, Dimitre - USDA-ARS<br> Nakhla, Mark - USDA-APHIS-CPHST<br> Pappu, Hanu - (hrp@wsu.edu) - Washington State Univ<br> Pesic-VanEsbroeck, Zvezdana (zvezdana@ncsu.edu) - NC State University<br> Postman, Joseph (Joseph.Postman@ars.usda.gov) - USDA-ARS<br> Poudyal, Dipak - Oregon Dept. Agriculture<br> Rabindran, Shailaja - USDA-APHIS-PPQ<br> Rascoe, John - USDA-APHIS-PPQ<br> Rayapati, Naidu (naidu.rayapati@wsu.edu) - Washington State Univ<br> Rott, Michael - Canadian Food Insp. Agency<br> Rowhani, Adib (akrowhani@ucdavis.edu) - Univ. of California-Davis<br> Rudyj, Erich - USDA-APHIS-NCPN<br> Schilder, Annemiek (schilder@msu.edu) - Michigan State Univ<br> Schmidt, Anna-mary (anna-mary.schmidt@inspection.gc.ca) - Canadian Food Insp. Agency<br> Scott, Simon (sscott@clemson.edu) - Clemson Univ.<br> Sharma-Poudyal, Dipak (dpoudyal@oda.state.or.us) - Oregon Dept. of Ag<br> Sivasankarapillai, Syamkumar - Washington State Univ<br> Sohrab, Bodaghi - Univ. California-Riverside<br> Sudarshana, Mysore (mysore.sudarshana@ars.usda.gov) - USDA-ARS<br> Susaimuthu, James - Driscolls, Inc<br> Tahir, Nouman - USDA-ARS<br> Tzanetakis, Ioannis (itzaneta@uark.edu) - University of Arkansas<br> Villamor, Dan - Washington State Univ<br> Welliver, Ruth (rwelliver@pa.gov) - PA Dept of Agriculture<br> Zhang, Shulu (shulu@agdia.com) - Agdia, Inc.<br> Vidalakis, Georgios - Univ. California-Riverside
WERA-20 Meeting
July 6-9, 2015
Please see the attached meeting minutes.
Individual member reports will be uploaded to the "Additional Documents" section on the WERA-20 Homepage once it is again available.
Accomplishments
Progress has been made in the conversation related to the application of NGS technology in quarantine systems that regulate trade between states and countries. Sensitive and reliable diagnostic assays that have been developed by WERA-20 members have been shared with labs of various federal and state agencies as well as centers of the National Clean Plant Network. Scientific collaborative efforts by members of the WERA-20 have brought together cutting-edge technologies to advance the detection and characterization of new and emerging viruses and virus-like agents from fruit trees, small fruits, citrus, and grapevines in the US and Canada.
Comparative studies have been conducted in various crop systems to compare and contrast the current bioassay systems with NGS for their relative sensitivity and reliability. This has resulted in finding molecular diversity of viruses using sensitive and high throughput diagnostic methodologies. Next-generation sequencing (NGS) technology has been used extensively in the past two years to identify new viruses and virus-like agents from grapevines, blueberries, pome and stone fruits.
Impacts
- This year, the WERA-20 working group, comprising members representing several states in the US, Canada, public and private universities, the USDA, and public and private sectors, brought together two very unique perspectives by having two additional day sessions. The first session was a summary of the current new technologies by scientists of the group, including comparative studies in which Next Generation Sequencing was compared with the current set of standard testing protocols.
- The second session was a conversation between scientists and regulators with the purpose of requesting guidelines from the regulators for the scientists to be able to implement the use of Next Generation Sequencing for quarantine purposes for the detection, characterization, and management of important virus diseases of perennial fruit crops.
- Virus and virus-like diseases are the major limiting factors for production of fruit trees, small fruits, citrus, and grapevines in the US and Canada. In 2015 The WERA 20 members have taken the lead in applying cutting-edge science and technology, including NGS for rapid identification of new and emerging viruses and deploying this information for increasing the information available so that this information may be used in the future for the purpose of improving quarantine and certification rules and regulations that will prevent the spread of exotic/emerging viruses across state and national borders.
- Members of the WERA-20 group have advanced the efforts towards the establishment of sensitive, reliable, and robust detection systems for viruses and virus-like agents by to identify viruses and worked on advancing efforts to eliminate pathogens in clean planting stock available for growers. Research efforts as well as advances in providing virus-tested planting materials to growers pursued by members of the WERA-20 have an important impact on the sustainability of fruit crops in the United States and Canada.
- In summary, state- of-the- art science and technology and science-based information generated by WERA-20 members shared with industry stakeholders and regulatory agencies is strengthening national efforts in safeguarding American agriculture for fruit crops from a wide range of virus and virus-like diseases, advancing sustainable growth of an important agricultural sector.
Publications
Akinbade SA, Mojtahedi H, Guerra L, Eastwell K, Villamor DEV, Handoo ZA, Skantar AM. 2014. First Report of Xiphinema rivesi (Nematoda, Longidoridae) in Washington State. Plant Disease 98: 1018.
Al Rwahnih, M., Rowhani, A., Golino, D., Islas, C., Preece, J., and Sudarshana, M.R. 2014. Detection and genetic diversity of Grapevine red blotch-associated virus isolates in table grape accessions in the National Clonal Germplasm Repository in California. Can. J. Plant Pathology 37:130–135.
Al Rwahnih, M., Daubert, S., Golino, D., Islas, C., and Rowhani, A. 2015. Comparison of Next Generation Sequencing vs. Biological Indexing for the Optimal Detection of Viral Pathogens in Grapevine. Phytopathology, in press.
Al Rwahnih, M., Rowhani, A., and Golino, D. First Report of Grapevine red blotch-associated virus in archival grapevine material from Sonoma County, California. Plant Disease, in press.
Al Rwahnih, M., Daubert, S., Golino, D., Durvasula, A., and Rowhani, A. 2015. Description and Detection of a novel Reovirus species in Cabernet grapevine in California. APS meeting, Pasadena CA, August 1-15, 2015.
Al Rwahnih, M., Daubert, S., Golino, D., and Rowhani, A. 2015. Next Generation Sequencing for the optimal detection of viral pathogens in Grapevine. APS meeting, Pasadena CA, August 1-15, 2015.
Al Rwahnih, M., Daubert, S., Golino, D., and Rowhani, A. Characterization of a Novel Reovirus Species in Cabernet Grapevine in California. Proceedings of the 18th Congress of ICVG, Ankara, Turkey. September 7-11, 2015
Al Rwahnih, M., Daubert, S., Golino, D., and Rowhani, A. Next-Generation Sequencing poised to replace Biological Indexing as the Gold Standard for Virus Detection in Grapevine. Proceedings of the 18th Congress of ICVG, Ankara, Turkey. September 7-11, 2015.
Al Rwahnih, M., Daubert, S., Islas, C., Golino, D. and Rowhani, A. 2014. Characterization of a fifth vitivirus in grapevine. J. Plant Pathol. 96: 219-222.
Alvarez, R.A., Martin, R.R. and Quito-Avila, D.F. 2015. First report of Pineapple mealybug wilt associated virus-1 in Ecuador. Plant Pathology 31:15.
Bag, S., Al Rwahnih, Li, A., Gonzalez, A., Rowhani, A., Uyemoto, J.K., and Sudarshana, M.R. 2015. Detection of a new luteovirus in imported nectarine trees: A case study to propose adoption of metagenomics in post-entry quarantine. Phytopathology 105:840-846.
Cao, Mengji, Lingling Pu, Margarita Bateman, Gary Kinard, Changyong Zhou and Ruhui Li. Simultaneous identification and molecular characterization of viruses associated with an apple tree. 2015. Abstract presented at the ICVF meeting in Japan, June 8-12, 2015
Cheong, Eun Ju, Chan-Soo Kim, Gary Kinard and Ruhui Li, 2015, Evaluation of the virus and viroid infection status of flowering cherry (Prunus yedoensis) collections in Korea and the U.S. J. Plant Path. 97: 155-160.
Cheong, Eun Ju, Ae Rin Jeon, Ray Mock, Gary Kinard and Ruhui Li. 2014. Elimination of Gooseberry vein banding associated virus by in vitro therapy. USDA-ARS-NGRL- PDRU-TT/2014-1.
Cheong, Eun Ju, Ae Rin Jeon, Jun Won Kang, Ray Mock, Gary Kinard and Ruhui Li. 2014. In vitro Elimination of Black raspberry necrosis virus from black raspberry (Rubus occidentalis). Hort. Sci. 41: 95–99.
Cheong, Eun Ju, Gary Kinard and Ruhui Li. 2014. Effect of carbohydrate sources on in vitro shoot growth of various Prunus species. USDA-ARS-NGRL-PDRU-TT/2014-3.
Dey, KK, Borth, WB, Melzer, MJ, Hu, JS. 2015. Application of circular polymerase extension cloning to generate infectious clones of a plant virus. Journal of Applied Biotechnology 3:34-44
Dey, K, Borth, W, Melzer, M, Wang, ML, Hu JS. 2015. Analysis of Pineapple mealybug wilt associated virus -1 and -2 for potential RNA silencing suppressors and pathogenicity factors. Viruses 7:969-995
Diaz-Lara, A., Mosier, N.J., Keller, K.E. and Martin, R.R. 2015. A variant of Rubus yellow net virus with altered genomic organization. Virus Genes 50:104-110.
Finn, C.E., Strik, B.C., Yorgey, B. and Martin, R.R. 2013. ‘Vintage’ red raspberry. HortScience 48:1181-1183.
Finn, C.E., Moore, P.P., Yorgey, B.M., Strik, B.C., Kempler, C., Dossett, M. and Martin, R.R. 2013. ‘Charm’ strawberry. HortScience 48:1184-1188.
Finn, C.E., Strik, B.C., Yorgey, B.M., Moore, P.P., Dossett, M., Kempler, C., Martin, R.R., Jamieson, A.R. and Galletta, G.J. 2014. ‘Sweet Sunrise’ strawberry. HortScience accepted 6.11.14
Fuchs, M., Marsella-Herrick, P., Hessler, S., Martinson, T. and Loeb, G. 2015. Seasonal acquisition of viruses by the grape mealybug, Pseudococcus maritimus (Erhorn), in a leafroll-diseased vineyard. Journal of Plant Pathology, in press.
Gergerich, R.C., Welliver, R., Gettys, S., Osterbauer, N.K., Kamenidou, S., Martin, R.R., Golino, D., Eastwell, K., Fuchs, M., Vidalakis, G. and Tzanetakis, I.E. 2015. Safeguarding fruit crops in the age of agricultural globalization. Plant Disease 99: 176- 187.
Golino, D., Rowhani, A., Klaassen, V., Sim, S., and Al Rwahnih, M. Grapevine Leafroll Associated Virus 1 Effects on Different Grapevine Rootstocks. Proceedings of the 18th Congress of ICVG, Ankara, Turkey. September 7-11, 2015.
Gottula, J., Lewis, R. Saito, S. and Fuchs, M. 2014. Allopolyploidy and the evolution of plant virus resistance. BMC Evolutionary Biology, 14:149.
Ho, T. and Tzanetakis, I.E. 2014. Developing a virus detection and discovery pipeline using next generation sequencing. Virology 471-473:54-60
James, D. 2014. Plum pox (Sharka); the disease and variability of the virus. University of California Plum Pox International Meeting, September 29 - October 1, 2014. UC-Davis, Davis, California, USA. http://ucanr.edu/sites/plumpox2014/Abstracts/James_Plum_pox_virus_variability/
James, D., Varga, A., Lye, D. 2014. Analysis of the complete genome of a virus associated with twisted leaf disease of cherry reveals evidence of a close relationship to unassigned viruses in the family Betaflexiviridae. Archives of Virology. 159: 2463-2468.
James, D., Phelan, J., Varga, A., Rott, M., Berube, J.A. 2015. First Report of Rose Cryptic Virus 1 in Rosa Plants in Canada. Plant Disease. 99: 558.
James, D. 2014. Plum pox virus (PPV) detection: the standardized IPPC diagnostic protocol. University of California Plum Pox International Meeting, September 29 - October 1, 2014. UC-Davis, Davis, California, USA. http://ucanr.edu/sites/plumpox2014/Abstracts/James_Plum_pox_virus_detection/
James, D., Green, C., and Wierenga, E. 2014. Plum Pox Monitoring and Management Program (PPMMP) in Canada. University of California Plum Pox International Meeting, September 29 - October 1, 2014. UC-Davis, Davis, California, USA. http://ucanr.edu/sites/plumpox2014/Abstracts/James_et_al_Program_in_Canada/
James, D., Phelan J. 2015. Detection and analysis of a filamentous virus isolated from black currant (Ribes nigrum cv. Baldwin) showing symptoms of leaf chlorosis and deformity. 23rd International Conference on Virus and Other Graft Transmissible Diseases of Fruit Crops, Morioka City, Japan, June 8 – 12, 2015. Book of Abstracts. Pg 67 (Abstr.).
James, D., Sanderson, D., Varga, A., Greig, N. and Stobbs, L.W. 2015. Analysis of the genetic diversity and relationships of selected Canadian isolates of Plum pox virus. Acta Hort. (ISHS) 1063:33-40.
James, D., Sanderson, D., Varga, A., Sheveleva, A., Chirkov, S. 2015. Recombination events may play an important role in the evolution of Plum pox virus (PPV). 23rd International Conference on Virus and Other Graft Transmissible Diseases of Fruit Crops, Morioka City, Japan, June 8 – 12, 2015. Book of Abstracts. Pg 52 (Abstr.).
Jinbo Song; Eric P. Benson; Patricia A. Zungoli; Patrick Gerard; Simon W. Scott. 2015. Using the DAS-ELISA test to establish an effective distance between bait stations for control of Linepithema humile (Hymenoptera: Formicidae) in natural areas. Journal of Economic Entomology doi: 10.1093/jee/tov152
Jones, T.J., Naidu, R.A., and Nita, M. 2015. Occurrence of Grapevine leafroll associated virus- 2, ?3 and Grapevine fleck virus in Virginia, U.S.A., and factors affecting virus infected vines. European Journal of Plant Pathology 142: 209-222.
Kalinowska, E., Marsella-Herrick, P. and Fuchs, M. 2015. Genetic variability of Blueberry scorch virus isolates from highbush blueberry in New York. Archives of Virology, 160:1537-1542.
Krenz, B., Thompson, J.R., McLane, H.L., Fuchs, M. and Perry, K.L. 2014. Grapevine red blotch-associated virus is widespread in the United States. Phytopathology, 104:1232- 1240.
Leal, I., Allen, E., Foord, B., Anema, J., Reisle, C., Uzunovic, A., Varga, A., James, D. 2014. Detection of living Bursaphelenchus xylophilus in wood, using reverse transcriptase loop-mediated isothermal amplification (RT-LAMP). Forest Pathology. 45: 134-148.
Lin, Liming, Ruhui Li, Ray Mock and Gary Kinard. 2014. Simultaneous Detection and Differentiation of Four Pome Viroids by RT-PCR. USDA-ARS-NGRL-PDRU-TT/2014- 2.
Long, MH, Ayin, C, Li, R, Hu, JS, Melzer, MJ. 2014. First report of taro vein chlorosis virus infecting taro [Colocasia esculenta (L.) Schott] in the United States of America. Plant Disease 98:1160
Martin, R.R. and Tzanetakis I.E. 2014. Control of virus diseases of berry crops. Advances in Virus Research 91:44-81.
Martin, R.R. and Tzanetakis, I.E. 2013. High risk strawberry viruses by region in the United States and Canada: Implications for certification, nurseries and fruit production. Plant Dis. 97:1358-1362.
Martin, R.R. and Tzanetakis I.E. 2015. Control of virus diseases of berry crops. Advances in Virus Research 91:271-309.
Mekuria, TA, Zhang S, Eastwell KC. 2014. Rapid and sensitive detection of Little cherry virus 2 using isothermal reverse transcription-recombinase polymerase amplification. Journal of Virological Methods 205:24-30.
Melzer MJ, Shimabukuro, JK., Long, M., Nelson, S., Alvarez, A., Borth, WB, Hu, JS. First report of Capsicum chlorosis virus infecting waxflower (Hoya calycina Schlecter) in the United States of America. 2014 Plant Disease 98:1160.
Moore, P.P., Barritt, B., Sjulin, T., Robbins, J.A., Finn, C.E., Martin, R.R. and Dossett, M. 2014. ‘Cascade Gold’ raspberry. HortScience 49:358-460.
Moore, P.P., Hoashi-Erhardt, W., Finn, C.E., Martin, R.R. and Dossett, M. 2015. ‘Cascade Harvest’ red raspberry. HortScience 50:24-627.
Naidu, R.A., 2014. Grapevine viruses and clean plants. In: Vine to Wine DVD. Edited by Gwen Hoheisel and Michelle Moyer (in press).
Naidu, R.A. 2014. Virus Diseases. In: 2015 Pest Management Guide for Grapes in Washington. WSU Extension Bulletin EB0762, pp.28-31.
Naidu, R.A., Maree, H.J., and Burger, J. 2015. Grapevine leaf roll disease and associated viruses – A unique pathosystem. Annual Review of Phytopathology 53 (in press).
Naidu, R.A., Rowhani, A., Fuchs, M., Golino, D., and Martelli, G.P. 2014. Grapevine leafroll: A complex viral disease affecting a high-value fruit crop. Plant Disease 98:1172-1185.
Naidu, R.A., Scharlau, V. 2014. Why ‘clean’ plants – Fact sheet (4 pages). Naidu, R.A. and Walsh, D. 2015. Is ‘grape virus tax’ hitting your pocketbook? Good Fruit Grower May 15, 2015. Vol. 66, No. 10, pages 10-11.
Osman, F., Al Rwahnih, M. and Rowhani, A. 2014. Improved detection of ilarviruses and nepoviruses affecting fruit trees using quantitative RT-qPCR. 2014. J. Plant Pathol. 96:577-583.
Postman, J.D. and H.R. Cameron. 1987. Apple mosaic virus in U.S. filbert germplasm. Plant Disease 71:944-945.
Postman, J.D. and S.A. Mehlenbacher. 1994. Apple mosaic virus in hazelnut germplasm. Acta Horticulturae 351:601-609.
Poudel, B., Ho, T., Laney, A., Khadgi, A. and Tzanetakis, I.E. 2014. Epidemiology of Blackberry chlorotic ringspot virus. Plant Disease 98:547-550.
Quito-Avila, D., Alvarez, R.A. and Martin, R.R. 2015. An umbra-like virus of papaya discovered in Ecuador: detection, occurrence and phylogenetic relatedness. EJPP (Dec. 9, 2014)
Quito-Avila, D.F., Brannen, P.M., Cline, W.O., Harmon, P.F. and Martin, R.R. 2013. Genetic characterization of Blueberry necrotic ring blotch virus, a novel RNA virus with unique genetic features. J. Gen. Virol. 94:1426-1434.
Quito-Avila, D.F., Ibarra, M.A., Alvarez, R.A., Espinoza, L., Ratti, M.F., Peralta, E.L. and Martin, R.R. 2013. First report of Raspberry bushy dwarf virus in the Andean blackberry (Rubus glaucus) in central Ecuador. Plant Dis. 97:1003.
Quito-Avila, D.F, Lightle, D. and Martin, R.R. 2014. Effect of Raspberry bushy dwarf virus, Raspberry leaf mottle virus, and Raspberry latent virus on plant growth and fruit crumbliness in ‘Meeker’ red raspberry. Plant Dis. 98:176-183.
Quito-Avila, D.F., Peralta, E.L., Ibarra, M.A., Alvarez, R. and Martin, R.R. 2014. A Raspberry bushy dwarf virus isolate from Ecuadorean Rubus glaucus contains an additional RNA that is a rearrangement of RNA 2. Arch. Virol. 159:2519-2521.
Rayapati, N., Rowhani, A., Fuchs, M., Golino, D. and Martelli, G.P. 2014. Grapevine leafroll: a complex viral disease affecting a high-value fruit crop. Plant Dis. 98: 1172-1185.
Ricketts, K.D., Gomez, M.I., Atallah, S.S., Fuchs. M.F., Martinson, T., Smith, R.J., Verdegaal, P.S., Cooper, M.L., Bettiga, L.J. and Battany, M.C. 2015. Reducing the economic impact of grapevine leafroll disease in California: identifying optimal management practices. American Journal of Enology and Viticulture, 66:138-147.
Rott, Michael, Mark Belton, Ian Boyes, Heidi Rast. Development of Next Generation Sequencing methods for plant virus diagnostics in grapevine and tree fruits. 11th International Conference of the European Foundation for Plant Pathology, Krakow, Sept 8-13, 2014.
Rott, Michael, Yurit Xiang, Michael Bernardy, Mark Belton, Ian Boyes, Heidi Rast, Cindy Tu, Edward Clarke, Bari Befeh Aadum. Analysis of Grapevine and Tree Fruit virus collections using Next Generation Sequencing. 11th International Conference of the European Foundation for Plant Pathology, Krakow, Sept 8-13, 2014.
Rowhani, A., Golino, D., Klaassen, V., Sim, S., Gouran, M., and Al Rwahnih, M. Grapevine Leafroll Associated Virus 3: Effects on Rootstocks, Vine Performance, Yield and Berries. Proceedings of the 18th Congress of ICVG, Ankara, Turkey. September 7-11, 2015.
Roy, A., Hartung, JS, Schneider, WL, Shao, J, Leon, MG, Melzer, MJ, Beard, JJ, Otero-Colina, G, Bauchan, GR, Ochoa, R, and Brlansky, RH. 201X. Role bending: complex relationships between viruses, hosts, and vectors related to citrus leprosis, and emerging disease. Phytopathology (in press).
Scott, S.W., MacFarlane, S.A. McGavin, W.J. and Fargette, D. 2014. Cassava Ivorian Bacilliform virus is a member of the genus Anulavirus. Archives of Virology 159: 159:2791–2793
Seguin, J., Rajeswaran, R., Malpica-López, N., Martin, R.R., Kasschau, K., Dolja, V.V., Otten, P., Farinelli, L. and Pooggin, M.M. 2014. De novo reconstruction of plant RNA and DNA virus quasispecies from siRNAs. PLoS One at http://dx.plos.org/10.1371/journal.pone.0088513
Skinkis, P., Pscheidt, J., Peachy, E., Dreves, A., Walton, V., Sanchez, D., Zasada, I. and Martin, R.R. 2014. Pest Management Guide for Wine Grapes in Oregon. Oregon State Univ. Ext. Bull. http://ir.library.oregonstate.edu/xmlui/bitstream/handle/1957/45975/em8413.pdf
Sudarshana, M.R., Perry, K.L., and Fuchs, M.F. 2015. Grapevine red blotch-associated virus, an emerging threat to the grapevine industry. Phytopathology 105: (In print).
Thekke-Veetil, T., Ho, T., Keller, K.E., Martin, R.R. and Tzanetakis, I.E. 2014. A new ophioivirus is associated with blueberry mosaic disease. Virus Research 189:92-96.
Thekke-Veetil, T., Ho, T., Keller, K.E., Martin, R.R. and Tzanetakis, I.E. 2014. A new ophiovirus associated with blueberry mosaic disease. Virus Research 189: 92-96.
Thompson, J.R., Fuchs, M., McLane, H., Toprak-Celebi, F., Fischer, K., Potter, J. and Perry, K.L. 2014. Profiling viral infections in grapevine using a randomly primed reverse transcription-polymerase chain reaction/macroarray multiplex platform. Phytopathology, 104:211-219.
Tzanetakis, I.E., Martin, R.R. and Wintermantel, W.M. 2013. Epidemiology of criniviruses, an emerging problem in world agriculture. Front. Microbiol. 4:119:1-15. doi: 10.3389/fmicb.2013.00119 Accepted April 26, 2013
Thekke-Veetil, T., Polashock, J.J., Marn, M.V., Plesko, I.M., Schilder, A.C., Keller, K.E., Martin, R.R. and Tzanetakis, I.E. 2015. Population structure of blueberry mosaic associated virus: Evidence of reassortment in geographically distinct isolates. Virus Research 201: 79-84.
Thekke-Veetil, T., Polashock, J., Marn, M.V., Plesko, I.M., Schilder, A., Keller, K.E., Martin, R.R. and Tzanetakis, I.E. 2015. Population structure of blueberry mosaic associated virus: Evidence of genetic exchange in geographically distinct isolates. Virus Res. 201:79-84.
Thekke-Veetil, T., Sabanadzovic, N. A-G., Keller, K.E., Martin, R.R., Sabanadzovic, S. and Tzanetakis, I.E. 2013. Molecular characterization and population structure of Blackberry vein banding associated virus, a new ampelovirus associated with blackberry yellow vein disease. Virus Res. 178:234-240.
Villamor DEV, Susaimuthu J, Eastwell KC. 2015. Genomic analyses of cherry rusty mottle group and cherry twisted leaf associated viruses reveal a possible new genus within the family Betaflexiviridae. Phytopathology 105:399-408.
Walker, L., Bagewadi, B., Schultz, A., and Naidu, R.A. 2015. First report of Tobacco ringspot virus associated with fanleaf disease in a Washington State vineyard. Plant Disease (in press).
Walker M, Chisholm J, Wei T, Ghoshal B, Saeed H, Rott M, Sanfaçon H. 2015. Complete genome sequence of three tomato ringspot virus isolates: evidence for reassortment and recombination. Arch Virol. 160(2):543-7.
Wallingford, A.K., Fuchs, M.F., Hessler, S., Martinson, T.M. and Loeb, G.M. 2015. Slowing the spread of grapevine leafroll-associated viruses in commercial vineyards with insecticide control of the vector, Pseudococcus maritimus (Erhorn) (Hemiptera: Pseudococcidae). Journal of Insect Science, in press.
Ward, N., Polashock, J., Thekke-Veetil, T., Martin, R.R. and Beale, J. 2015. First report of blueberry mosaic disease caused by Blueberry mosaic associated virus in Kentucky. Plant Dis. 99:421
Zhang S, Ravelonandrob M, Russell P, McOwen N, Briard P, Bohannon S, Vrient A (2014) Rapid diagnostic detection of plum pox virus in Prunus plants by isothermal AmplifyRP® using reverse transcription-recombinase polymerase amplification. Journal of Virological Methods 207:114–120.
Zhang S, Ravelonandrob M, Chambers M, Briard P, Masson M , Amato M, Vrient A (2015) Rapid diagnostic detection of plum pox virus by isothermal AmplifyRP® and by ImmunoStrip®. Acta Hort 1063:167-172.
Zhang S, Russell P, McOwen N, Bohannon S, Davenport B (2015) Development of a novel isothermal AmplifyRP method combining both real-time and endpoint assays in single tubes for rapid detection of plant pathogens. American Phytopathological Society Annual Meeting, August 1-5, 2015, California.