Brief Summary of Minutes of Annual Meeting

To view all reports from the 2015 NRSP-6 TAC meeting, please go to http://www.ars-grin.gov/nr6/NRSP6_TAC_MINUTES_2015_FINAL_PACKAGE.pdf

Our annual report can be accessed far below at the publication attachment link. A. Acquisitions and associated work In 2014, we collected 18 germplasm accessions from Arizona under the BdRF (Bamberg, del Rio, Fernandez) prefix, with the kind support of K. Williams of the USDA Plant Exploration office at Beltsville. This trip followed the Mogollon Rim from Springerville to Flagstaff. We found robust populations at new sites quite separated from any previous collections, and extending the range of S. fendleri north by about 50 miles. The detailed trip report is available on request and on GRIN. We also sought and received 15 new cultivars and breeding clones from cooperators (La Rouge, Igorota, Amey, Peter Wilcox, Harley Blackwell, Sandy, Sylvia (CPVPA), Marine, Cynthia, (ver x cph) -8-1, (ver x cmm) -1, -2, -21-1; (ver x pnt) -1, -2. The NRSP-6 web page (http://www.ars-grin.gov/nr6) was updated to include all new stocks and screening information. Clients who have ordered from NRSP-6 within the past four years were contacted three times in 2014, informing them of new stocks of true seed, tubers, in vitro plantlets, or other samples. We used email and the website to extend technical instructions of various types. For example, we produced and selected the best 10% of seedling tubers in the winter at Davis, CA (see at right and insert). When planted in Wisconsin the following summer, they far outperformed random seedlings, allowing us to skip one field selection season. B. Preservation and Evaluation This year, 218 accessions were increased as botanical seed populations and 2,700 clonally. Over 700 potato virus tests were performed on seed increase parents, seedlots and research materials. Germination tests were performed on 1523 accessions, ploidy determinations were made on 23 accessions, and tetrazolium seed viability tests were done on 63 seedlots. Taxonomic status was assessed on all stocks grown. Nearly 4,000 individual field plots, greenhouse and screenhouse growouts were done locally, at the research farm at Hancock, WI, or with cooperators at Davis, CA. We initiated a new test of winter field growouts for tuber evaluation with cooperators at Hastings, FL and Yuma, AZ. We continued breeding for improved Criolla or "egg yolk" style specialty potato with golden flesh (cooperator Douglass from FL). We completed a project using SNPs (cooperator Douches from MI) to assess partitioning of genetic diversity in model potato species with a view to understanding their best management. We evaluated for heat stress tolerance (cooperators at Parlier, CA), folate (cooperator Goyer from OR), glycoalkaloids (cooperator Navarre from WA), anti-obesity (cooperator Kemin from IA), new Double Corolla mutant, Zebra Chip resistance in bulbocastanum (with R. Cooper in WA). We tested all S. verrucosum accessions for crossability to S. jamesii through mentor pollination. C. Classification Dr. Spooner is working on monographs that will fully document the taxonomic reduction of the genebank's holdings to about 100 species. D. Distribution service Distribution of germplasm is at the heart of our service. The volume and types of stocks sent to various consignee categories are summarized in the table below. In 2014, we had 200 domestic orders to clients in 37 states and 18 foreign orders to 9 other countries. Half of the domestic orders are for breeding and genetics, 1/4 for home gardeners, and remainder 1/4 for pathology, physiology, entomology, taxonomy and education. In 2014 we maintained the popular offering of 100 cultivars as tubers by devising and implementing an iron-clad disease control and quarantine program for their production (full details available at our website). E. Outreach Media coverage, Tours, Teaching, TechTran and Trips with presentations done Summer student interns participated in experiments: Ahna Keilar (seed germ and seedling transplant tech), Abe Keilar (crossing tech for jam and blb), Hannah Haight (prebiotic assay and nutrient microbial bioassays), Rosa Lozano, (Colombian student visitor for Criolla potatoes). Potato Association of America meeting in Spokane--research presentations/abstracts. Chinese, Russian, and Japanese potato scientists, UW River Falls Horticulture students, and Southern Door HS Spanish class tour genebank. Leadership: Bamberg continued as Editor in Chief for the American Journal of Potato Research, and Chair of the USDA/ARS Potato Crop Germplasm Committee. Reports & Plans: ARS: PGOC, CGC, CRIS, Annual Performance, Budget. NRSP6: Annual Report, TAC meeting minutes, Project Renewal FY16-20. PAA: AJPR Editor in Chief report. UW-Hort: Annual Performance. PARS: Tour guide & field book. Management of Grants & Awards: Potato CGC grants and AJPR Outstanding Paper.

Arbizu, C., H. Ruess, D. Senalik, P. Simon, and D. M. Spooner. 2014. Phylogenomics of the carrot genus (Daucus, Apiaceae). Amer. J. Bot. 101:1666-1685. Arbizu, C., K. R. Reitsma, P. W. Simon, and D. M. Spooner. 2014. Morphometrics of Daucus (Apiaceae): A counterpart to a phylogenomic study. Amer. J. Bot. 101:2005-2016. Bamberg, J., Moehninsi, R. Navarre, and J. Suriano. 2015. Variation for Tuber Greening in the Diploid Wild Potato Solanum microdontum. American Journal of Potato Research 92:435-443. Bamberg, JB, A del Rio, D Douches, and J Coombs. 2014. Assessing SNPs for predicting heterogeneity and screening efficiency in wild potato species. American Journal of Potato Research (submitted 08/20/14). Bamberg, JB. and AH del Rio. 2014. Selection and Validation of an AFLP Marker Core Collection for the Wild Potato Solanum microdontum. American Journal of Potato Research 91:368-375. Bamerg, JB, J Suriano, A del Rio, WR Cooper, J Abad and C.Fernandez. 2014. Matryoshka: A New Floral Mutant in Potato. American Journal of Potato Research: 91:500-503. Bethke, P, N Atef, S Kubow, Y Leclerc, X Li, M Haroon, T Molen, JB Bamberg, M Martin and D Donnelly. 2014. History and Origin of Russet Burbank (Netted Gem) a sport of Russet Burbank. American Journal of Potato Research 91:579-593. Castañeda-Álvarez, N. P., de Haan, S., Juárez, H., Khoury, C. K., Achicanoy, H. A., Sosa, C. C., Bernau, V., Salas A., Heider, B., Maxted, N., and Spooner, D. M. 2015. Ex situ conservation priorities for the wild relatives of potato (Solanum L. section Petota). PLoS ONE 10(4): e0122599. doi:10.1371/journal.pone.0122599. Clausen, A., I. Peralta, and D. M. Spooner. 2014. Solanum section Petota. Flora of Argentina, vol. 13, Solanaceae, Ed. Fernando Zuloaga and Manuel Belgrano, Instituto Multidisciplinario de Biología Vegetal (CONICET-UNC), Buenos Aires, Argentina. Cooper, WR and JB Bamberg. 2014. Variation in Bactericera cockerelli (Hemiptera: Triozidae) oviposition, survival, and development on Solanum bulbocastanum germplasm. American Journal of Potato Research 91:532-537. Hardigan, M., J Bamberg, C Robin Buell and D Douches. 2015. Taxonomy and genetic differentiation among wild and cultivated germplasm of Solanum sect. Petota. The Plant Genome. 8:1:16. Jansky, S. and A. Hamernik. 2014. Rapid cycling of potato tuber generations by overcoming dormancy. American Journal of Potato Research. DOI 10.1007/s12230-014-9415-7. Jansky, S. H., D. M. Spooner, and P. Bethke. 2014. Yield gains in US potato, contributing factors and future prospects, pp 195-217. In: S. Smith (ed.), Yield Gains in Major U.S. Field Crops: Contributing Factors and Future Prospects. Crop Science Society of America, Madison, WI. Jansky, S. H., J. Dawson, and D. M. Spooner. 2015. How do we address the disconnect between genetic and morphological diversity in germplasm collections? American Journal of Botany: In press. Jansky, S.H. and D.A. Fajardo. 2014. Tuber starch amylose content is associated with cold-induced sweetening in potato. Food Science and Nutrition. Doi: doi: 10.1002/fsn3.137. Jansky, S.H., P. C. Bethke, and D. M. Spooner. 2014. Yield gains in potato: Contributing factors and future prospects. In: Yield Gains in Major U.S. Field Crops. Ed. S. Smith, B. Diers, B. Carver, and J. Specht. CSSA, Madison, WI. Jansky, S.H., Y.S. Chung, and P. Kittipadukal. 2014. M6: A diploid inbred line for use in breeding and genetics research. Journal of Plant Registrations. doi: 10.3198/jpr2013.05.0024crg. Khiutti, A., D. M. Spooner, S. H. Jansky, and D. A. Halterman. 2015. Testing taxonomic predictivity of foliar and tuber resistance to Phytophthora infestans in wild relatives of potato. Phytopathology: In press. Limantseva, L., Mironenko, N., Shulalov, O., Antonova, O., Khiutti, A., Novikova, L., Afanasenko, O., D. Spooner, and T. Gavrilenko. 2014. Characterization of resistance to Globodera rostochiensis pathotype Ro1 in cultivated and wild potato species accessions from the Vavilov Institute of Plant Industry. Plant Breeding 133: 660–665. Mezghani, N., I. Zaouali, W. Wided Bel Amri, S. Rouz. P. W. Simon, C. Hannachi, Z. Ghrabi, M. Neffati, B. Bouzbida, and D. M. Spooner. 2014. Fruit morphological descriptors as a tool for discrimination of Daucus L. germplasm. Genet. Res. Crop Evol. 61: 499-510. Särkinen, T., M. Baden, P. Gonzáles, M. Cueva, L. L. Giacomin, D. M. Spooner, R. Simon, H. Juárez, P. Nina, J. Molina, and S. Knapp 2015. Listado anotado de Solanum L. (Solanaceae) en el Perú (Annotated checklist of Solanum L. (Solanaceae) for Peru). Revista Peruana de Biología 22(1): 3-62. Spooner, D. M. In press. Solanum section Petota. In: Flora of North America. Spooner, D. M., and P. W. Simon. In press. Daucus. In: A Flora of Oregon; Stephen C. Meyers (ed.); Oregon State University Press Spooner, D. M., and P. W. Simon. In press. Daucus. In: Flora of North America. Spooner, D. M., M. Ghislain, R. Simon, S. H. Jansky, and T. Gavrilenko. 2014. Systematics, diversity, genetics, and evolution of wild and cultivated potatoes. Bot. Rev. 80: 283-383. Spooner, D. M., M. P. Widrlechner, K. R. Reitsma, D. E. Palmquist, S. Rouz, Z. Ghrabi-Gammar, M. Neffati, B. Bouzbida, H. Ouabbou, M. El Koudrim, and P. W. Simon. 2014. Reassessment of practical subspecies identifications of the USDA Daucus carota germplasm collection: Morphological data. Crop Science 54: 706-718. Spooner, D.M. and J.F. Pruski. In press. Simsia Persoon for Flora Mesoamericana (Flora series published by Missouri Botanical Garden), Natural History Museum, London, and the National Autonomous University, Mexico City. Spooner, D.M. In press. Solanum L. section Petota Dumort for Flora Mesoamericana. (Flora series published by Missouri Botanical Garden), Natural History Museum, London, and the National Autonomous University, Mexico City. Spooner, D.M., M. Guislain, R. Simon, S.H. Jansky, and T. Gavrilenko. 2014. Systematics, diversity, and evolution of wild and cultivated potatoes. Botanical Review. In press. Uribe, P., S. Jansky, and D. Halterman. 2014. Two CAPS markers predict Verticillium wilt resistance in wild Solanum species. Molecular Breeding. 33:465-476. Zorrilla, C, F Navarro, S Vega, JB Bamberg and JP Palta. 2014. Identification and Selection for Tuber Calcium, Internal Quality and Pitted Scab in Segregating Atlantic x Superior reciprocal tetraploid populations. American Journal of Potato Research 91:673-687.