SAES-422 Multistate Research Activity Accomplishments Report
Sections
Status: Approved
Basic Information
- Project No. and Title: NC1004 : Genetic and Functional Genomic Approaches to Improve Production and Quality of Pork
- Period Covered: 10/01/2002 to 05/01/2003
- Date of Report: 06/05/2003
- Annual Meeting Dates: 05/30/2003 to 05/31/2003
Participants
Baas, Tom - Iowa State University,tjbaas@iastate.edu; Bates, Ron - Michigan State University, batesr@msu.edu; Beattie, Craig - University of Nevada-Reno, cbeattie@cabur.unr.edu; Beever, Jon - University of Illinois, j-beever@uiuc.edu; Brayton, Peter - USDA-CSREES, pbrayton@csrees.usda.gov; Cassady, Joe - North Carolina State University, jpcassady@ncsu.edu; Dekkers, Jack - Iowa State University, jdekkers@iastate.edu; DeSilva, Udaya - Oklahoma State University, udayad@okstate.edu; Ernst, Cathy - Michigan State University, ernstc@msu.edu; Fahrenkrug, Scott - University of Minnesota, fahre001@umn.edu; Firat, Mehmet Z. - Iowa State University, mzf@iastate.edu; Freking, Brad - USDA-ARS-MARC, freking@email.marc.usda.gov; Gere, Tiber - St. Stephen Univ. Hungary, j-zsuzsa@freemail.hu; Holl, Justin - University of Nebraska-Lincoln, jholl1@bigred.unl.edu; Hovey, Russ - University of Vermont, rhovey@zoo.uvm.edu; Johnson, Rodger - University of Nebraska-Lincoln, Rjohnson5@unl.edu; Kim, Jong Joo - Iowa State University, kimjj@iastate.edu; Lee, Go Hyun - Iowa State University, leegh.123@iastate.edu; Lunney, Joan - USDA-ARS-BARC, jlunney@anri.barc.usda.gov; Moeller, Steve - Ohio State University, moeller.29@osu.edu; Moody, Diane - Purdue University, moodyd@purdue.edu; Mote, Benny - Iowa State University, bmote@iastate.edu; Newcom, Doug - Iowa State University, dnewcom@iastate.edu; Petry, Derek - University of Nebraska-Lincoln, dpetry@unlserve.unl.edu; Pomp, Daniel - University of Nebraska-Lincoln, dpomp@unl.edu; Robison, O.W. - North Carolina State Univrsity, OW_Robison@nscu.edu, Rothschild, Max - Iowa State University, mfrothsc@iastate.edu; Schwab, Clint - Iowa State Universtiy, crschwab@iastate.edu; Stromberg, Bert - University of Minnesota, b-stro@umn.edu; Tuggle, Chris - Iowa State University, cktuggle@iastate.edu; Weber, Jack - University of Nebraska-Lincoln, jweber4@unl.edu; Zhang, Glenn - Oklahoma State University, zguolon@okstate.edu; Zhao, Shuhong - Iowa State University, shzhao@iastate.edu
[Minutes]
Accomplishments
The NC-1004 Regional Project (Genetic and functional genomic approaches to improve production and quality of pork) met on May 30-31 for their annual meeting. This represented the groups first meeting since approval of the project, and covered the period of October 1, 2002 to May 31, 2003. Given the brief period of time that the group was in existence prior to the meeting, a significant amount of effort was placed on learning more about individual efforts ongoing at participating stations, taking stock of resources and reagents that could be used for regional project activities, and strategic planning for future group endeavors and collaborations.
A major accomplishment that the group has already realized is the deposit of well over 100,000 expressed sequenced tags (EST) into the public genetic database (GenBank). This includes ~85,000 contributed from ARS efforts, and ~30,000 from university stations.
A second major accomplishment is the planning and development of a swine oligonucleotide microarray representing over 10,000 unique expressed genes. A limited number of arrays are available to participating members of NC-1004, while reagents to create larger numbers of arrays are publicly available for purchase.
Key strategic planning accomplishments included development of two sub-committees for the project, including the NC-1004 Bioinformatics Working Committee and the NC-1004 Outreach Committee.
The existence of extensive and well developed populations, genomics reagents and bioinformatics tools represents the raw materials with which the group plans to achieve significant accomplishments with important outcomes during the course of the 5-year project. A thorough inventory of existing resources was conducted to provide the base from which regional project collaborations can be developed. A summary of these (sorted by category and station) include:
US Swine Genome Coordinator Resources (M.F. Rothschild)
1) DD-PCR (limited supply) and microsatellite primers
2) Oligonucleotide microarrays
3) Database resources (EST, QTL)
4) BAC libraries and filters
cDNA Libraries
1) Illinois Three skeletal muscle libraries (various stages)
2) Iowa 21 libraries (embryos and reproductive tissues at various stages)
3) Michigan skeletal muscle, brain, mixed tissue
4) Nebraska ovarian follicle
5) MARC Four mixed tissue libraries
Populations
1) Illinois Berkshire X Duroc F2; DNA and tissues available on collaborative basis
2) Iowa Berkshire X Yorkshire F2; Advanced intercrosses currently at F6; map candidate genes on collaborative basis
3) Michigan Duroc X Pietrain F2; DNA and tissues available on collaborative basis
4) Minnesota Developing Duroc X Yorkshire/Landrace reciprocal backcross population
5) Nebraska Litter size selection lines; DNA available
6) North Carolina Duroc testosterone selection lines, Litter size selection lines, PRRS induction model (B. Harrell); DNA and tissues available on collaborative basis
7) MARC White Composite selection lines; serially slaughtering 600 gilts at 5 stages of gestation and could collect tissues on a collaborative basis
Mapping DNA Resources
1) IMpRH (available from M. Rutherford at U. of MN)
2) PiGMaP (available from M. Rothschild; supplies limited)
3) MARC
Bioinformatics
1) Indiana database for microarrays
2) Iowa EST and QTL databases
3) Michigan EST database and microarray data analysis
4) Minnesota data analysis software tools
In summary. significant progress has already been made towards achieving several of the outputs by which progress of the regional project will be measured. In additional, several of the milestones for Year 1 have been met although significantly less than a full year has elapsed since the project became active.
Plans for the coming year include extensive use of resource populations and genomics reagents for integrated gene discovery, expansion of bioinformatic tools to aid in the discovery process, and an emphasis on outreach efforts to extend discoveries to the industry.
A major accomplishment that the group has already realized is the deposit of well over 100,000 expressed sequenced tags (EST) into the public genetic database (GenBank). This includes ~85,000 contributed from ARS efforts, and ~30,000 from university stations.
A second major accomplishment is the planning and development of a swine oligonucleotide microarray representing over 10,000 unique expressed genes. A limited number of arrays are available to participating members of NC-1004, while reagents to create larger numbers of arrays are publicly available for purchase.
Key strategic planning accomplishments included development of two sub-committees for the project, including the NC-1004 Bioinformatics Working Committee and the NC-1004 Outreach Committee.
The existence of extensive and well developed populations, genomics reagents and bioinformatics tools represents the raw materials with which the group plans to achieve significant accomplishments with important outcomes during the course of the 5-year project. A thorough inventory of existing resources was conducted to provide the base from which regional project collaborations can be developed. A summary of these (sorted by category and station) include:
US Swine Genome Coordinator Resources (M.F. Rothschild)
1) DD-PCR (limited supply) and microsatellite primers
2) Oligonucleotide microarrays
3) Database resources (EST, QTL)
4) BAC libraries and filters
cDNA Libraries
1) Illinois Three skeletal muscle libraries (various stages)
2) Iowa 21 libraries (embryos and reproductive tissues at various stages)
3) Michigan skeletal muscle, brain, mixed tissue
4) Nebraska ovarian follicle
5) MARC Four mixed tissue libraries
Populations
1) Illinois Berkshire X Duroc F2; DNA and tissues available on collaborative basis
2) Iowa Berkshire X Yorkshire F2; Advanced intercrosses currently at F6; map candidate genes on collaborative basis
3) Michigan Duroc X Pietrain F2; DNA and tissues available on collaborative basis
4) Minnesota Developing Duroc X Yorkshire/Landrace reciprocal backcross population
5) Nebraska Litter size selection lines; DNA available
6) North Carolina Duroc testosterone selection lines, Litter size selection lines, PRRS induction model (B. Harrell); DNA and tissues available on collaborative basis
7) MARC White Composite selection lines; serially slaughtering 600 gilts at 5 stages of gestation and could collect tissues on a collaborative basis
Mapping DNA Resources
1) IMpRH (available from M. Rutherford at U. of MN)
2) PiGMaP (available from M. Rothschild; supplies limited)
3) MARC
Bioinformatics
1) Indiana database for microarrays
2) Iowa EST and QTL databases
3) Michigan EST database and microarray data analysis
4) Minnesota data analysis software tools
In summary. significant progress has already been made towards achieving several of the outputs by which progress of the regional project will be measured. In additional, several of the milestones for Year 1 have been met although significantly less than a full year has elapsed since the project became active.
Plans for the coming year include extensive use of resource populations and genomics reagents for integrated gene discovery, expansion of bioinformatic tools to aid in the discovery process, and an emphasis on outreach efforts to extend discoveries to the industry.
Impacts
- Publicly available genomic data bases have been greatly enhanced by provision of information from the Regional Project, allowing other researchers and industry to capitalize on developed information and methods.
- Progress towards new methodologies and technologies for genetic improvement of pigs has been made, setting the stage for substantial advances in production efficiency and product quality, including increased litter size, improved growth rate and feed efficiency, and improved product quality.
Publications
INDIANA
Bidwell, C.A., M. Spurlock, and D. Moody. 2003. Serial analysis of gene expression of pig adipose and muscle tissue. Plant and Animal Genomes XI Conference, San Diego, CA. www.intl-pag.org/11/abstracts/P01_P69_XI.html.
IOWA
Berg, E. P., E. L. McFadin, K. R. Maddock, R. N. Goodwin, T. J. Baas, and D. H. Keisler. 2003. Serum concentrations of leptin in six genetic lines of swine and relationship with growth and carcass characteristics. J. Anim. Sci. 81:167-171.
Chaiwong, N., J.C.M. Dekkers, R.L. Fernando and M.F. Rothschild. 2003. Introgressing Multiple QTL through Backcross Breeding Programs. Swine Research Reports. Iowa State University.
Chen, P., T. J. Baas, J. W. Mabry, J. C. M. Dekkers, and K. J. Koehler. 2002. Genetic parameters and trends for lean growth rate and its components in U. S. Yorkshire, Duroc, Hampshire, and Landrace pigs. J. Anim. Sci. 80:2062-2070.
Chen, P., T. J. Baas, J. W. Mabry, J. C. M. Dekkers, and K. J. Koehler. 2003. Genetic parameters and trends for litter traits in U. S. Yorkshire, Duroc, Hampshire, and Landrace pigs. J. Anim. Sci. 81:46-53.
Ciobanu, D.C., S.M. Lonergan, M. Malek, J.R. Woollard, E.J. Lonergan and M.F. Rothschild. 2003. New Alleles in the Calpastatin Gene Associated with Improved Tenderness in Pigs. Swine Research Reports. Iowa State University
Crock, A.M., D.S. Casey, and J.C.M. Dekkers. 2003. Feeding behavior of finishing boars and gilts on electronic feeders. J. Anim. Sci. 81 (Suppl. 2):
Kim, K.S., N. T. Nguyen, J. M Reecy, L. L. Anderson, and M. F. Rothschild. 2003. Molecular genetic studies of porcine genes for obesity. Swine Research Reports. Iowa State University.
Kulak, K., J. Wilton, G. Fox and J. Dekkers. 2003. Comparisons of economic values with and without risk for livestock trait improvement Livestock Production Science, 79: 183-191.
Malek, M., D. C. Ciobanu, and M. F. Rothschild. 2003. Genetic and physical mapping of ACACB, PPP1CC and GPR49 genes on porcine chromosomes 5 and 14 help to define a break point on human chromosome 12. Proceedings Plant & Animal Genome XI meeting, January 11-15, San Diego, CA.
Moeller, S. J., T. J. Baas, T. D. Leeds, R. S. Emnett, and K. M. Irvin. 2003. Rendement Napole gene effects and a comparison of glycolytic potential and DNA genotyping for classification of Rendement Napole status in Hampshire-sired pigs. J. Anim. Sci. 81:402-410.
Newcom, D. W., T. J. Baas, and J. W. Lampe. 2002. Prediction of intramuscular fat percentage in live swine using real-time ultrasound. J. Anim. Sci. 80:3046-3052.
Newcom, D. W., T. J. Baas, and R. N. Goodwin. 2003. Relationship between intramuscular fat percentage predicted from real-time ultrasound and meat quality traits in pigs. Midwest ASAS Abstracts, p. 5.
Newcom, D. W., T. J. Baas, J. W. Mabry, and R. N. Goodwin. 2002. Genetic parameters for pork carcass components. J. Anim. Sci. 80:3099-3106.
Nguyen, N-T, K.-S. Kim, H. Thomsen, J. Helm and M. F. Rothschild 2003. Investigation of candidate gene for growth and fatness QTL on the pig chromosome 7. Proceedings Plant & Animal Genome XI meeting, January 11-15 San Diego, CA.
Otieno, C.J., S. Vleck, C. Jelks, K.-S. Kim, N. T. Nguyen and M. F. Rothschild. 2003. Mapping of diabetes-related genes in the pig. Proceedings Plant & Animal Genome XI meeting, January 11-15, San Diego, CA.
Rothschild, M.F. 2002. Approaches and limitations to measuring genetic diversity. EEC sponsored Conference on Swine Genetic Diversity, November 7, Cordoba, Spain.
Rothschild, M.F. 2002. Transferring Biotechnology to Swine Genetic Programs: From Lab to Pork. Spanish Swine Breeders Conference. November 5, Barcelona, Spain
Rothschild, M.F. and S. Newman. 2002. Intellectual property rights in animal breeding and genetics. CABI Press. 272 pages
Schwab, C. R., T. J. Baas, S. J. Moeller, and D. W. Newcom. 2003. Accuracy of prediction in the National Swine Improvement Federation (NSIF) Ultrasound Certification Program. Midwest ASAS Abstracts, p. 14.
Shi, X.W., Y.D. Zhang, and C.K. Tuggle. 2002. Linkage mapping of porcine STCH further refines the HSA3/21 breakpoint on pig chromosome 13. Anim. Genet. 33:395-396.
Sun, H.S., C.K. Tuggle, A. Goureau, C.J. Fitzsimmons, P. Chardon, P. Pinton, and M. Yerle. 2002. Precise mapping of breakpoints in conserved synteny between human chromosome 1 and pig chromosomes 4, 6, and 9. Anim. Gen. 33:91-96.
Sun, H.S., L. L. Anderson, T.-P. Yu, K.S. Kim, J. Klindt, and C. K. Tuggle. 2002. Neonatal Meishan pigs show POU1F1 genotype effects on plasma GH and PRL concentration. Anim. Reprod. Sci. 69:223-237.
Thomsen, H., J. C. M. Dekkers, and M. F. Rothschild. 2003. Detection and characterization of QTL for growth and meat quality traits in the Berkshire-Yorkshire cross. Swine Research Reports. Iowa State University.
Wang, J., J. Fulton, and J. Dekkers. 2003. Accuracy of detecting quantitative trait loci by selective DNA pooling. J. Anim. Sci. 81 (Suppl. 2):
Wang, J., K. Koehler, M. Soller, and J.C.M. Dekkers. 2003. Least squares interval mapping to detect QTL with selective DNA pooling. Plant and Animal Genome XI: 69.
MICHIGAN
Farber, C.R., N.E. Raney, D.L. Kuhlers, K. Nadarajah and C.W. Ernst. 2003. Mapping of porcine genetic markers generated by representational difference analysis. Anim. Biotech. 14:87-102.
Yao, J., P.M. Coussens, P. Saama, S. Suchyta and C.W. Ernst. 2002. Generation of expressed sequence tags from a normalized porcine skeletal muscle cDNA library. Anim. Biotech. 13:211-202.
Zhao, S-H., D. Nettleton, W. Liu, C.W. Fitzsimmons, C.W. Ernst N.E. Raney, and C.K. Tuggle. 2003. cDNA macroarray analysis of differential gene expression in porcine fetal and postnatal muscle. J. Anim. Sci. (Accepted)
Allison, C.P., R.O. Bates, A.M. Booren, R.C. Johnson and M.E. Doumit. 2003. Pork quality is not explained by activity of rate-limiting glycolytic enzymes. Meat Science 93:17-22.
Bates, R.O., D.B. Edwards and R.L. Korthals. 2003. Sow performance when housed in groups with electronic sow feeders or stalls. Livestock Production Science 79:29-35.
Bates, R.O., M.D. Hoge. B.E. Edwards and B. Straw. 2003. The influence of canine teeth clipping on nursing and nursery pig performance. Swine Health and Production 11(2):75-80.
Edwards, D.B., R.O. Bates and W.N. Osburn. 2003. Evaluation of Duroc- vs Pietrain-sired pigs for carcass and meat quality measures. J. Anim. Sci. 81: (Accepted).
Pagan, M. M.E. Davis, D.A. Stick, R.C.M. Simmen, N.E. Raney, R.J. Tempelman and C.W. Ernst. 2003. Evaluation of serum insulin-like growth factor binding proteins (IGFBP) in Angus cattle divergently selected for serum IGF-I concentration. Domest. Anim. Endocrinol. (Accepted).
NORTH CAROLINA
Holl, J. W. and O. W. Robison. 2003. Results from nine generations of selection for increased litter size in swine. J. Anim Sci. 81:624629, March 2003.
Blowe, C. D., E. J. Eisen, O. W. Robison, and J. P. Cassady. Characterization of a line of pigs selected for increased litter size for genes known to affect reproduction. J. Anim. Sci. 81(Suppl 1): 56, March 2003.
Walker, S., O. W. Robison, C. S. Whisnant, and J. P. Cassady. Effect of selection for testosterone production on testicular morphology and daily sperm production in pigs. J. Anim Sci.. 81(Suppl 1): 5, March 2003.
Cassady, J. P., K. A. Leymaster, and L. D. Young. Heterosis and recombination effects on pig reproductive traits. J. Anim Sci. 80(9):2286-2302, Sept. 2002
Cassady, J. P., K. A. Leymaster, and L. D. Young. Heterosis and recombination effects on pig growth and carcass traits. J. Anim Sci. 80(9):2303-2315, Sept. 2002.
OHIO
Stoller G. M., H. N. Zerby, S. J. Moeller, T. J. Baas, C. Johnson, and L. E. Watkins. 2003. The effect of feeding Ractopamine (Payleand) on muscle quality and sensory characteristics in three diverse genetic lines of swine. J. Anim. Sci. 81:1508-1516.
Moeller, S. J., T. J. Baas, T. D. Leeds, R. S. Emnett, and K. M. Irvin. 2003. Rendement Napole gene effects and a comparison of glycolytic potential and DNA genotyping for classification of Rendement Napole status in Hampshire-sired pigs. J. Anim. Sci. 81:402-410.
Isler, B. J., K. M. Irvin, S. M. Neal, S. J. Moeller, and M. E. Davis. 2002. Examination of the relationship between the estrogen receptor gene and reproductive traits in swine. J. Anim. Sci. 80:2334-2339.
NEBRASKA
Bertani G, Johnson RK, Pomp D (2003) Mapping of porcine ESTs putatively differentially expressed in anterior pituitary Animal Genetics 34:132-134.
Caetano A, RK Johnson, D Pomp (2003) Generation and Sequence Characterization of a Normalized cDNA Library from Swine Ovarian Follicles. Mammalian Genome 14:65-70.
Childs KD, DW Goad, MF Allan, D Pomp, C Krehbiel, RD Geisert, JB Morgan, JR Malayer (2002) Expression of the NAT1 translational repressor in intramuscular adipocytes of Angus X Hereford steers. Physiological Genomics 10:49-56.
Chiang, C. F., R. K. Johnson, and M. K. Nielsen. 2002. Maternal Behavior in Mice Selected for Large Litter Size. Appl. Anim. Behavior Sci. 79: 63-73.
Chiang, C. F., R. K. Johnson, and M. K. Nielsen. 2002. Selection for Maternal Behavior in Mice Direct and Correlated Responses. Appl. Anim. Behaviour Sci. 79: 311-323.
Gladney C, G Bertani, MK Nielsen, D Pomp (2003) Evaluation of gene expression in pigs selected for enhanced reproduction. I: Ovarian Follicles. J Anim Sci (In Revision).
Leamy L, Pomp D, Eisen EJ, Cheverud J (2002) Pleiotropy of quantitative trait loci for organ weights and limb bone lengths in mice. Physiol. Genomics 10:21-29.
Mesa, H. t. J. Safranski, R. K. Johnson, and W. R. Lamberson. 2003. Correlated response in placental efficiency in swine selected for an index of components of litter size. J. Anim. Sci. 81: 74-79.
Risatti GR, D Pomp, RO Donis (2003) Patterns of cellular gene expression in cells infected with cytopathic or non-cytopathic bovine diarrhea virus. Animal Biotechnology (In Press).
Tuggle C, Green JA, Fitzsimmons C, Woods R, Prather RS, Malchenko S, Soares MB, Tack D, Robinson N, O‘Leary B, Scheetz T, Casavant T, Pomp D, Edeal JB, Zhang Y, Rothschild MF, Garwood K, Beavis W (2003) EST-Based Gene Discovery in Pig: Virtual Expression Patterns and Comparative Mapping to Human. Mammalian Genome (In Press).
Wesolowski S, Allan MF, Nielsen MK, Pomp D (2003) Evaluation of hypothalamic gene expression in mice selected for heat loss. Physiological Genomics 13:129-137.
Williams RW, Broman KW, Cheverud JM, Churchill GA, Hitzemann RW, Hunter Kw, Muntz
JD, Pomp P, Reeves RH, Schalkwyk LC, Threadgill DW (2002) A collaborative cross for high
precision complex trait analysis. www.complextrait.org/Workshop1.pdf
Ziemba EA, R. K. Johnson, and g. E. Duhamel. 2002. Identification of Brachyspira pilosicoli attachment phenotypes among purebred and crossbred pigs as determined by epithelial cell brush border adhesion. CRWAD, St. Louis, MO.
Ziemba EA, E. M. Berberov, C. J. Stryker, R. Zhang, R. K. Johnson, and G. E. Duhamel. Attachment of Brachyspira pilosicoli to Pig Intestinal Brush Borders: Identification of Three Pig Phenotypes. CRWAD. Nov 12-13, St. Louis, MO.
OKLAHOMA
C. R. Krehbiel, S. R. Rust, G. Zhang, and S. E. Gilliland. 2003. Bacterial Direct-Fed Microbials in Ruminant Diets: Performance Response and Mode of Action. J. Anim. Sci. (In press).
USDA, ARS, BARC
Lunney, J.K. 2003. In Search of Disease-Resistant Pigs. National Hog Farmer, Apr 15, 2003, pp.30-34.
Martens G W, Lunney J K, Baker J E, Smith D M. 2003. Rapid Assignment of Swine Leukocyte Antigen (SLA) Haplotypes in Pedigreed Herds using a Polymerase Chain Reaction Based Assay. Immunogenetics. In Press.
Lunney JK, Fossum C, Alm GV, Steinbach F, Wattrang E. 2002. Veterinary immunology: opportunities and challenges. Trends Immunol. 23: 4-6.
Solano-Aguilar, G.I., Zarlenga, D., Beshah, E., Vengroski, K, Gasbarre, L., Junker, D., Cochran, M., Weston, C., Valencia, D., Chiang, C., Dawson H, Urban J, and Lunney, J.K. 2002. Limited effect of recombinant porcine Interleukin-12 on porcine lymphocytes due to a low expression of IL-122 receptor. Vet. Immunol. Immunopathol. 89: 133-148.
Bidwell, C.A., M. Spurlock, and D. Moody. 2003. Serial analysis of gene expression of pig adipose and muscle tissue. Plant and Animal Genomes XI Conference, San Diego, CA. www.intl-pag.org/11/abstracts/P01_P69_XI.html.
IOWA
Berg, E. P., E. L. McFadin, K. R. Maddock, R. N. Goodwin, T. J. Baas, and D. H. Keisler. 2003. Serum concentrations of leptin in six genetic lines of swine and relationship with growth and carcass characteristics. J. Anim. Sci. 81:167-171.
Chaiwong, N., J.C.M. Dekkers, R.L. Fernando and M.F. Rothschild. 2003. Introgressing Multiple QTL through Backcross Breeding Programs. Swine Research Reports. Iowa State University.
Chen, P., T. J. Baas, J. W. Mabry, J. C. M. Dekkers, and K. J. Koehler. 2002. Genetic parameters and trends for lean growth rate and its components in U. S. Yorkshire, Duroc, Hampshire, and Landrace pigs. J. Anim. Sci. 80:2062-2070.
Chen, P., T. J. Baas, J. W. Mabry, J. C. M. Dekkers, and K. J. Koehler. 2003. Genetic parameters and trends for litter traits in U. S. Yorkshire, Duroc, Hampshire, and Landrace pigs. J. Anim. Sci. 81:46-53.
Ciobanu, D.C., S.M. Lonergan, M. Malek, J.R. Woollard, E.J. Lonergan and M.F. Rothschild. 2003. New Alleles in the Calpastatin Gene Associated with Improved Tenderness in Pigs. Swine Research Reports. Iowa State University
Crock, A.M., D.S. Casey, and J.C.M. Dekkers. 2003. Feeding behavior of finishing boars and gilts on electronic feeders. J. Anim. Sci. 81 (Suppl. 2):
Kim, K.S., N. T. Nguyen, J. M Reecy, L. L. Anderson, and M. F. Rothschild. 2003. Molecular genetic studies of porcine genes for obesity. Swine Research Reports. Iowa State University.
Kulak, K., J. Wilton, G. Fox and J. Dekkers. 2003. Comparisons of economic values with and without risk for livestock trait improvement Livestock Production Science, 79: 183-191.
Malek, M., D. C. Ciobanu, and M. F. Rothschild. 2003. Genetic and physical mapping of ACACB, PPP1CC and GPR49 genes on porcine chromosomes 5 and 14 help to define a break point on human chromosome 12. Proceedings Plant & Animal Genome XI meeting, January 11-15, San Diego, CA.
Moeller, S. J., T. J. Baas, T. D. Leeds, R. S. Emnett, and K. M. Irvin. 2003. Rendement Napole gene effects and a comparison of glycolytic potential and DNA genotyping for classification of Rendement Napole status in Hampshire-sired pigs. J. Anim. Sci. 81:402-410.
Newcom, D. W., T. J. Baas, and J. W. Lampe. 2002. Prediction of intramuscular fat percentage in live swine using real-time ultrasound. J. Anim. Sci. 80:3046-3052.
Newcom, D. W., T. J. Baas, and R. N. Goodwin. 2003. Relationship between intramuscular fat percentage predicted from real-time ultrasound and meat quality traits in pigs. Midwest ASAS Abstracts, p. 5.
Newcom, D. W., T. J. Baas, J. W. Mabry, and R. N. Goodwin. 2002. Genetic parameters for pork carcass components. J. Anim. Sci. 80:3099-3106.
Nguyen, N-T, K.-S. Kim, H. Thomsen, J. Helm and M. F. Rothschild 2003. Investigation of candidate gene for growth and fatness QTL on the pig chromosome 7. Proceedings Plant & Animal Genome XI meeting, January 11-15 San Diego, CA.
Otieno, C.J., S. Vleck, C. Jelks, K.-S. Kim, N. T. Nguyen and M. F. Rothschild. 2003. Mapping of diabetes-related genes in the pig. Proceedings Plant & Animal Genome XI meeting, January 11-15, San Diego, CA.
Rothschild, M.F. 2002. Approaches and limitations to measuring genetic diversity. EEC sponsored Conference on Swine Genetic Diversity, November 7, Cordoba, Spain.
Rothschild, M.F. 2002. Transferring Biotechnology to Swine Genetic Programs: From Lab to Pork. Spanish Swine Breeders Conference. November 5, Barcelona, Spain
Rothschild, M.F. and S. Newman. 2002. Intellectual property rights in animal breeding and genetics. CABI Press. 272 pages
Schwab, C. R., T. J. Baas, S. J. Moeller, and D. W. Newcom. 2003. Accuracy of prediction in the National Swine Improvement Federation (NSIF) Ultrasound Certification Program. Midwest ASAS Abstracts, p. 14.
Shi, X.W., Y.D. Zhang, and C.K. Tuggle. 2002. Linkage mapping of porcine STCH further refines the HSA3/21 breakpoint on pig chromosome 13. Anim. Genet. 33:395-396.
Sun, H.S., C.K. Tuggle, A. Goureau, C.J. Fitzsimmons, P. Chardon, P. Pinton, and M. Yerle. 2002. Precise mapping of breakpoints in conserved synteny between human chromosome 1 and pig chromosomes 4, 6, and 9. Anim. Gen. 33:91-96.
Sun, H.S., L. L. Anderson, T.-P. Yu, K.S. Kim, J. Klindt, and C. K. Tuggle. 2002. Neonatal Meishan pigs show POU1F1 genotype effects on plasma GH and PRL concentration. Anim. Reprod. Sci. 69:223-237.
Thomsen, H., J. C. M. Dekkers, and M. F. Rothschild. 2003. Detection and characterization of QTL for growth and meat quality traits in the Berkshire-Yorkshire cross. Swine Research Reports. Iowa State University.
Wang, J., J. Fulton, and J. Dekkers. 2003. Accuracy of detecting quantitative trait loci by selective DNA pooling. J. Anim. Sci. 81 (Suppl. 2):
Wang, J., K. Koehler, M. Soller, and J.C.M. Dekkers. 2003. Least squares interval mapping to detect QTL with selective DNA pooling. Plant and Animal Genome XI: 69.
MICHIGAN
Farber, C.R., N.E. Raney, D.L. Kuhlers, K. Nadarajah and C.W. Ernst. 2003. Mapping of porcine genetic markers generated by representational difference analysis. Anim. Biotech. 14:87-102.
Yao, J., P.M. Coussens, P. Saama, S. Suchyta and C.W. Ernst. 2002. Generation of expressed sequence tags from a normalized porcine skeletal muscle cDNA library. Anim. Biotech. 13:211-202.
Zhao, S-H., D. Nettleton, W. Liu, C.W. Fitzsimmons, C.W. Ernst N.E. Raney, and C.K. Tuggle. 2003. cDNA macroarray analysis of differential gene expression in porcine fetal and postnatal muscle. J. Anim. Sci. (Accepted)
Allison, C.P., R.O. Bates, A.M. Booren, R.C. Johnson and M.E. Doumit. 2003. Pork quality is not explained by activity of rate-limiting glycolytic enzymes. Meat Science 93:17-22.
Bates, R.O., D.B. Edwards and R.L. Korthals. 2003. Sow performance when housed in groups with electronic sow feeders or stalls. Livestock Production Science 79:29-35.
Bates, R.O., M.D. Hoge. B.E. Edwards and B. Straw. 2003. The influence of canine teeth clipping on nursing and nursery pig performance. Swine Health and Production 11(2):75-80.
Edwards, D.B., R.O. Bates and W.N. Osburn. 2003. Evaluation of Duroc- vs Pietrain-sired pigs for carcass and meat quality measures. J. Anim. Sci. 81: (Accepted).
Pagan, M. M.E. Davis, D.A. Stick, R.C.M. Simmen, N.E. Raney, R.J. Tempelman and C.W. Ernst. 2003. Evaluation of serum insulin-like growth factor binding proteins (IGFBP) in Angus cattle divergently selected for serum IGF-I concentration. Domest. Anim. Endocrinol. (Accepted).
NORTH CAROLINA
Holl, J. W. and O. W. Robison. 2003. Results from nine generations of selection for increased litter size in swine. J. Anim Sci. 81:624629, March 2003.
Blowe, C. D., E. J. Eisen, O. W. Robison, and J. P. Cassady. Characterization of a line of pigs selected for increased litter size for genes known to affect reproduction. J. Anim. Sci. 81(Suppl 1): 56, March 2003.
Walker, S., O. W. Robison, C. S. Whisnant, and J. P. Cassady. Effect of selection for testosterone production on testicular morphology and daily sperm production in pigs. J. Anim Sci.. 81(Suppl 1): 5, March 2003.
Cassady, J. P., K. A. Leymaster, and L. D. Young. Heterosis and recombination effects on pig reproductive traits. J. Anim Sci. 80(9):2286-2302, Sept. 2002
Cassady, J. P., K. A. Leymaster, and L. D. Young. Heterosis and recombination effects on pig growth and carcass traits. J. Anim Sci. 80(9):2303-2315, Sept. 2002.
OHIO
Stoller G. M., H. N. Zerby, S. J. Moeller, T. J. Baas, C. Johnson, and L. E. Watkins. 2003. The effect of feeding Ractopamine (Payleand) on muscle quality and sensory characteristics in three diverse genetic lines of swine. J. Anim. Sci. 81:1508-1516.
Moeller, S. J., T. J. Baas, T. D. Leeds, R. S. Emnett, and K. M. Irvin. 2003. Rendement Napole gene effects and a comparison of glycolytic potential and DNA genotyping for classification of Rendement Napole status in Hampshire-sired pigs. J. Anim. Sci. 81:402-410.
Isler, B. J., K. M. Irvin, S. M. Neal, S. J. Moeller, and M. E. Davis. 2002. Examination of the relationship between the estrogen receptor gene and reproductive traits in swine. J. Anim. Sci. 80:2334-2339.
NEBRASKA
Bertani G, Johnson RK, Pomp D (2003) Mapping of porcine ESTs putatively differentially expressed in anterior pituitary Animal Genetics 34:132-134.
Caetano A, RK Johnson, D Pomp (2003) Generation and Sequence Characterization of a Normalized cDNA Library from Swine Ovarian Follicles. Mammalian Genome 14:65-70.
Childs KD, DW Goad, MF Allan, D Pomp, C Krehbiel, RD Geisert, JB Morgan, JR Malayer (2002) Expression of the NAT1 translational repressor in intramuscular adipocytes of Angus X Hereford steers. Physiological Genomics 10:49-56.
Chiang, C. F., R. K. Johnson, and M. K. Nielsen. 2002. Maternal Behavior in Mice Selected for Large Litter Size. Appl. Anim. Behavior Sci. 79: 63-73.
Chiang, C. F., R. K. Johnson, and M. K. Nielsen. 2002. Selection for Maternal Behavior in Mice Direct and Correlated Responses. Appl. Anim. Behaviour Sci. 79: 311-323.
Gladney C, G Bertani, MK Nielsen, D Pomp (2003) Evaluation of gene expression in pigs selected for enhanced reproduction. I: Ovarian Follicles. J Anim Sci (In Revision).
Leamy L, Pomp D, Eisen EJ, Cheverud J (2002) Pleiotropy of quantitative trait loci for organ weights and limb bone lengths in mice. Physiol. Genomics 10:21-29.
Mesa, H. t. J. Safranski, R. K. Johnson, and W. R. Lamberson. 2003. Correlated response in placental efficiency in swine selected for an index of components of litter size. J. Anim. Sci. 81: 74-79.
Risatti GR, D Pomp, RO Donis (2003) Patterns of cellular gene expression in cells infected with cytopathic or non-cytopathic bovine diarrhea virus. Animal Biotechnology (In Press).
Tuggle C, Green JA, Fitzsimmons C, Woods R, Prather RS, Malchenko S, Soares MB, Tack D, Robinson N, O‘Leary B, Scheetz T, Casavant T, Pomp D, Edeal JB, Zhang Y, Rothschild MF, Garwood K, Beavis W (2003) EST-Based Gene Discovery in Pig: Virtual Expression Patterns and Comparative Mapping to Human. Mammalian Genome (In Press).
Wesolowski S, Allan MF, Nielsen MK, Pomp D (2003) Evaluation of hypothalamic gene expression in mice selected for heat loss. Physiological Genomics 13:129-137.
Williams RW, Broman KW, Cheverud JM, Churchill GA, Hitzemann RW, Hunter Kw, Muntz
JD, Pomp P, Reeves RH, Schalkwyk LC, Threadgill DW (2002) A collaborative cross for high
precision complex trait analysis. www.complextrait.org/Workshop1.pdf
Ziemba EA, R. K. Johnson, and g. E. Duhamel. 2002. Identification of Brachyspira pilosicoli attachment phenotypes among purebred and crossbred pigs as determined by epithelial cell brush border adhesion. CRWAD, St. Louis, MO.
Ziemba EA, E. M. Berberov, C. J. Stryker, R. Zhang, R. K. Johnson, and G. E. Duhamel. Attachment of Brachyspira pilosicoli to Pig Intestinal Brush Borders: Identification of Three Pig Phenotypes. CRWAD. Nov 12-13, St. Louis, MO.
OKLAHOMA
C. R. Krehbiel, S. R. Rust, G. Zhang, and S. E. Gilliland. 2003. Bacterial Direct-Fed Microbials in Ruminant Diets: Performance Response and Mode of Action. J. Anim. Sci. (In press).
USDA, ARS, BARC
Lunney, J.K. 2003. In Search of Disease-Resistant Pigs. National Hog Farmer, Apr 15, 2003, pp.30-34.
Martens G W, Lunney J K, Baker J E, Smith D M. 2003. Rapid Assignment of Swine Leukocyte Antigen (SLA) Haplotypes in Pedigreed Herds using a Polymerase Chain Reaction Based Assay. Immunogenetics. In Press.
Lunney JK, Fossum C, Alm GV, Steinbach F, Wattrang E. 2002. Veterinary immunology: opportunities and challenges. Trends Immunol. 23: 4-6.
Solano-Aguilar, G.I., Zarlenga, D., Beshah, E., Vengroski, K, Gasbarre, L., Junker, D., Cochran, M., Weston, C., Valencia, D., Chiang, C., Dawson H, Urban J, and Lunney, J.K. 2002. Limited effect of recombinant porcine Interleukin-12 on porcine lymphocytes due to a low expression of IL-122 receptor. Vet. Immunol. Immunopathol. 89: 133-148.