SAES-422 Multistate Research Activity Accomplishments Report

Status: Approved

Basic Information

Participants

Baker, John [baker@anr.msu.edu] Michigan Blomberg, LeAnn [leann.blomberg@ars.usda.gov] USDA-ARS-ABBL (visitor) Caperna, Tom [Thomas.caperna@ ars.usda.gov] USDA-ARS-ABBL (visitor) Davis, Duane [davis@ksu.edu] Kansas Diekman, Mark [mdiekman@purdue.edu] Indiana Geisert, Rodney [geisertr@missouti.edu] Missouri Guthrie, H. David [dave.guthrie@ars.usda.gov] USDA-BARC Kerns, Karl [kkerns@iastate.edu] Iowa State/USDA-ARS (visitor) Miles, Jeremy [jeremy.miles@ars.usda.gov] USDA-MARC Mirando, Mark [mmirando@nifa.usda.gov] USDA-NIFA Ross, Jason [jwross@iastate.edu] Iowa Safranski, Tim [safranskit@missouri.edu] Missouri Welcl, Glenn [glenn.Welcl@ ars.usda.gov] USDA-ARS-ABBL (visitor) White, Brett [bwhite2@unlnotes.unl.edu] Nebraska Wilson, Matt [mwilso25@wvu.edu] West Virginia

The 2013 annual meeting of the NCERA57 was held at Beltsville, MD in room 003 of Building 003 on the Beltsville Agricultural Research Center campus. The meeting was called to order by Dave Guthrie, Vice Chair and local host at 8:00 AM on Tuesday, May 21st. Dave Guthrie made opening remarks and introduced the Director of BARC, Dr. Steven R. Shafer. Dr. Shafer gave a brief history of BARC and indicated that it is the largest Animal and Plant Center in the US with currently about 180 scientists. Research at BARC involves not only animal and plant studies but food safety and human nutrition. The campus has a rich history dating almost 100 years. Programs have opened the fields of genomics and molecular techniques to increase milk production as well as the first transgenic growth hormone pigs were developed at BARC. Dr. Mark Mirando, National Program Leader for Animal Nutrition and Growth and Reproduction within the National Institute of Food and Agriculture (NIFA), attended the meeting and provided a NIFA update which included the announcement of the appointment of Dr. Sonny Ramaswamy as the new Director of NIFA on May 7, 2012. Dr. Adele Turzillo, National Program Leader for Animal Production Systems, was promoted to Director, Institute of Animal Systems April 8, 2013. The Animal Reproduction FY2013 proposals have been reviewed but notifications will not go out until the recent federal budget funding has been approved through the NIFA programs. Dr. Mirando indicated the opportunity for funding through the Dual Purpose with Dual Benefit: Research in Biomedicine and Agriculture Using Agriculturally Important Domestic Species program. Next application deadline is September 24, 2013. Following the NIFA update, station reports were given by Dr. Brett White (Nebraska), Jeremy Miles (USMARC), Duane Davis (Kansas), Rod Geisert (Missouri), and Jason Ross (Iowa). Following lunch, Jason Ross led a discussion for the establishment of the 2014 NCERA-57 Symposium presentation speakers and committee. Location of symposium was discussed and committee agreed that the symposium will be held in Lincoln, NE with the annual meeting of the NCERA-057 group. The meeting in Lincoln, NE would coincide with the anniversary of the opening of MARC in Clay Center, NE which would be a special program with the research group at MARC. Previous topics for the symposium were reviewed by Dr. Ross and the group selected to have the symposium topic cover Improved Litter Size and Environmental Effects on Pig Quality. Possible speakers included Dr. Clay Lents (USDA-ARS-MARC), Billy Flowers (North Carolina State) and Roger Johnson (University of Nebraska). Brett White (University of Nebraska), Jeremy Miles (USDA-MARC), Tim Safranski (University of Missouri) and Jason Ross (Iowa State University) were selected to form the committee to develop the symposium and sponsors. The symposium will be held May 20, 2014. Dr. John Parrish provided the station report for Wisconsin and the meeting was adjourned for dinner. On Wednesday May 22, Dr. John Baker conducted the business meeting for the NCERA-57 committee. Dr. Baker praised the organization and accomplishments of the NCERA057 committee and indicated that this specific committee was among the easiest to advise and the most focused in the program. He stated that the NCERA057 committee very accurately conducts the purposes intended for this program. For the 2013-2014 officers, Jason Ross will step down as Chairman and Dave Guthrie will become the new Chairman with Rod Geisert filling the position of Vice Chairman. The group selected and approved John Parrish as secretary. Dave Guthrie was also finally added as a member of the NCERA-57 group and Rod Geisert still needs to be placed on the committee by his station. John Baker indicated that group was on the 4th year of the program and needed to develop a rewrite for the next 5 year renewal. The objectives for the proposal are to be loaded into the NIMSS are due October 15, 2013, all participants and AES must submit Appendix E forms by November 15 and the complete proposal is due in the NIMSS system by December 1. The group selected John Parrish (Wisconsin) to chair the committee to write the renewal proposal with Duane Davis (Kansas) and Rod Geisert (Missouri). The committee will work on objectives which will be email to the group for editing and approval. The group discussed adding new members to the NCERA-57 participants. John Baker will extend invitations to Lea Rempel (USDA-MARC), Frank White (Cameron University, Cameron, OK), Greg Johnson (Texas A&M) and Kim Vonnahme (North Dakota State University) to participate in the 2014 annual meeting. Dr. Jeremy Miles discussed developing an integrated USDA grant on Seasonal Fertility which would focus on boars. There is good strength in the male, semen, seasonality and fertility within members of the NCERA-57 group. The goal would be to develop a good proposal for the RFP on Climate Change. The proposal would investigate the use of frozen versus extend semen form boar studs during summer/winter. Possible objectives would be to evaluate sperm morphology (techniques from John Parrish lab), pregnancy rates, and epigenetic effects through methylation of DNA in the fetus and placenta. A committee consisting of Jeremy Miles (USDA-MARC), Lea Rempel (USDA-MARC), John Parrish (Wisconsin), Tim Safranski (Missouri) and Duane Davis (Kansas) were selected to develop the proposal. Dr. Dave Guthrie provided the last station report for USDA-BARC. The meeting was adjourned and Dave provided a tour of the research buildings and laboratories on the Beltsville (BARC) campus. The meeting was adjourned at 12:05 PM. respectfully submitted, Rod Geisert, Secretary, NCERA57

Accomplishments

1. Numerous publications were published by committee members and their associates during the past year that provide scientific knowledge and impact on reproductive efficiency in swine. 2. The topic, location and program committee for the 2014 NCERA-57 Symposium on Improved Litter Size and Environmental Effects on Pig Quality was completed. 3. The group discussed the preliminary experiments at USMARC to design, write, and submit a NIFA Integrated Grant proposal to support a study of the relative contributions of the male to seasonal infertility in swine using frozen semen. A committee to develop and write the proposal was formed to submit the completed proposal AFRI Foundational Program on Climate Change when the RFP is released. 4. A committee was selected to write the 5 year renewal for the NCERA-57 program.

Impacts

  1. A functional GnRHR-II has been discovered in pigs and recent evidence suggests that GnRH-II may be directly involved in testosterone production, as boars immunized against GnRH-II displayed reduced plasma testosterone, independent of changes in luteinizing hormone levels. GnRH-II receptors have been localized in to the plasma membrane of Leydig cells. The role of GnRH-II in the development of boar testis and semen production will be evaluated through knockdown the production of gonadotropin-releasing hormone II receptor. A GnRH II knockout gilt has been produced which will help serve as a founder for production of males.
  2. Pig conceptuses secrete a unique form of interleukin-1beta which has been proposed to alter uterine function and initiate rapid trophoblast elongation prior to placental attachment. A IL-1B knockout is current being developed to determine the role of the cytokine in the establishment of pregnancy.
  3. Breeding of pigs with frozen semen may help reduce the seasonal depression in fertility. There is question of whether frozen semen can have any epigenetic effects on offspring. Methylation analysis indicates that there was little epigenetic modification to the fetus but changes in placental genome methylation was detected in a primary study.
  4. Methods of improving classroom learning can be enhanced through laboratory data analysis. One method is to illustrate the effects of fetal crowding on brain development evaluate fetal growth at different stages of pregnancy. The fetal response to nutritional stress leads to brain sparing as measured by an increase in the ratio of brain to liver weight. A second method is to demonstrate delayed implantation in rats with an estrogen antagonist which provides discussion on not only delayed implantation but hormones and receptor function. Third, development of case studies to introduce solving reproductive problems in an international animal program outside the US will help students to become more culturally aware and outside their comfort zone.
  5. US MARC continues to have an eight week student training program to introduce students to agricultural research.
  6. Heat stress of sows during gestation causes epigenetic effects on offspring which alters adolescent offspring body composition indices and metabolic parameters in pigs.
  7. The understanding of miRNA expression on development in pigs may facilitate the development of strategies to improve the reproductive efficiency in pigs. In addition, understanding the specific mechanisms of heat stress during gestation in pigs will be beneficial in characterizing the year-round implications of periods of environmental stress.
  8. The artificial induction on heat stress for 48 h in boars through boar pants allowed the determination of when heat has a detrimental effect on spermatogenesis. Increase in testis temperature effected development of primary spermatocytes. The shape of the sperm head is an indicator of damage and fertility.
  9. A zinc-regulated membrane pore Staphylococcus aureus ±-hemolysin transports small impermeant protectants into sperm. Hypothesis is that intracellular transport of impermeant small molecules will better protect cellular organelles and improve the longevity and fertility of sperm during hypothermic storage in either liquid or frozen form.
  10. Analysis of ejaculated sperm residual RNA may provide information for prediction of fertility, storage longevity, and early embryo development. However, there are problems with getting enough non-degraded RNA from the sperm preps.

Publications

Aad PY, Echternkamp SE, Sypherd DD, Schreiber NB, Spicer LJ. 2012. The hedgehog system in ovarian follicles of cattle selected for twin ovulations and births: evidence of a link between the IGF and hedgehog systems. Biology of Reproduction 87(4):79. Aarabi M, Yu Y, Xu W, Man Y, Tse MY, Pang SC, Yi Y-J, Sutovsky P, Oko R. 2012. The Testicular and epididymal expression profile of PLC¶ in mouse and human does not support its role as a sperm-borne oocyte activating factor. PLoS ONE, 7(3):e33496. Ashworth MD, Ross JW, Ritchey JR, DeSilva U, Stein DR, Geisert RD, White FJ. 2012. Effects of Aberrant Estrogen on the Endometrial Transcriptional Profile in Pigs. Reproductive Toxicology 2012 34:8-15. Aslam ML, Bastiaansen JW, Elferink MG, Megens HJ, Crooijmans RP, Blomberg LA, Fleischer RC, Van Tassell CP, Sonstegard TS, Schroeder SG, Groenen MA, Long JA. 2012. Whole genome SNP discovery and analysis of genetic diversity in Turkey (Meleagris gallopavo). BMC Genomics 14:13:391. doi: 10.1186/1471-2164-13-391. Bakst MR, Akuffo V, Nicholson D, French N. 2012. Comparison of blastoderm traits from 2 lines of broilers before and after egg storage and incubation. Poultry Science 91:2645-2648. Bartol FF, Wiley AA, Miller DJ, Silva AJ, Roberts KE, Davolt MLP, Chen JC, Frankshun, A-L, Camp ME, Rahman KM, Vallet JL, Bagnell CA. 2013. Lactation Biology Symposium: Lactocrine signaling and developmental programming. Journal of Animal Science 91(2):696-705. Baumgard, LH, Rhoads RP, Rhoads ML, Gabler NK, Ross JW, Keating AF, Boddicker RL, Lenka S, Sejian V. 2012. Impact of climate change on livestock production. Chapter 15. Environmental Stress and Amelioration in Livestock Production. Eds. V. Sejian, S.M.K. Naqvi, T. Ezeji, J. Lakritz, and R. Lal. Springer Publishing, pp 413-468. Bhattacharya P, Madden JA, Sen N, Hoyer PB, Keating AF. 2013. Glutathione S-transferase class mu regulation of apoptosis signal-related kinase 1 protein during VCD-induced ovotoxicity in neonatal rat ovaries. Toxicology and Applied Pharmacology 267(1):49-56. Bi J, Li Y, Sun F, Saalbach A, Klein C, Miller DJ, Hess R, Nowak R. 2013. Basigin null mutant male mice are sterile and exhibit impaired interactions between gametes and Sertoli cells. Developmental Biology (In Press). Bischoff SR, Tsai SQ, Hardison NE, Motsinger-Reif AA, Freking BA, Nonneman DJ, Rohrer GA, Piedrahita JA. 2013. Differences in X-chromosome transcriptional activity and cholesterol metabolism between placentae from swine breeds from Asian and Western origins. PloS One 8(1):E55345. Blomberg LA, Telugu BP. 2012. Twenty years of embryonic stem cell research in farm animals. Reproduction in Domestic Animals 47 Suppl: 4:80-85. Breen, SM, Knox RV. 2012. The impact of dose of FSH (Follitropin) containing LH (Lutropin) on follicular development, estrus and ovulation responses in prepubertal gilts. Animal Reproductive Science 132:193-200. Canaday DC, Salak-Johnson JL, Visconti AM, Wang X, Bhalerao K, Knox RV. 2013. Effect of variability in lighting and temperature environments for mature gilts house in gestation crates on measures of reproduction and animal well-being. Journal of Animal Science 91:1225-1236. Caperna TJ, Shannon AE, Blomberg LA, Garrett WM, Ramsay TG. 2012. Iron dextran treatment does not induce serum protein carbonyls in the newborn pig. Animal 6:79-86. Caperna TJ, Shannon AE, Blomberg LA, Stoll M, Ramsay TG. 2012. Identification of alpha-1 acid glycoprotein (AGP) as a potential marker of impaired growth in the newborn piglet. Reproduction, Fertility and Development 10.1071/RD12103. [Epub ahead of print] Caires KC, de Avila JM, Cupp AS, McLean DJ. 2012. VEGFA family isoforms regulate spermatogonial stem cell homeostasis in vivo. Endocrinology 153:887-900. Coleman, SW, Chase CC, Phillips WA, Riley DG, Olson TA. 2012. Evaluation of tropically adapted straightbred and crossbred cattle: Postweaning BW gain and feed efficiency when finished in a temperate climate. Journal of Animal Science 90(6):1955-1965. Cushman RA. 2013. Physiology and Endocrinology Symposium: The current status of heat shock in early embryonic survival and reproductive efficiency. Journal of Animal Science 91(3):1141-1142. Cushman RA, Perry GA. 2012. What we know about the genetics of reproduction. Applied Reproductive Strategies in Beef Cattle (Proc., Sioux Falls, SD; 3-4 Dec 2012):165-173. Echternkamp SE, Aad PY, Eborn DR, Spicer LJ. 2012. Increased abundance of aromatase and follicle stimulating hormone receptor mRNA and decreased insulin-like growth factor-2 receptor mRNA in small ovarian follicles of cattle selected for twin births. Journal of Animal Science 90(7):2193-2200. Flowers WL. 2013. Sperm characteristics that limit success of fertilization. Journal of Animal Science (in press). Flowers WL, Stewart KR, Gall T, Novack S, Dyck MK, Kirkwood RN. 2013. Boar seminal plasma proteins and their relevance to reproductive technologies. In: Control of Pig Reproduction IX, H. Rodriguez-Martinez, N. Soede, and W.L. Flowers (eds). Context Publishing, Ltd., Nottingham, U.K. pp. 33-46. Foust KB, Li Y, Park KE, Liu S, Wang X, Cabot RA. 2013. The polycomb group protein EED varies in its ability to access the nucleus in porcine oocytes and cleavage stage embryos. Animal Reproduction Science 133(3-4):198-204. Frankel TE, Theisen DD, Guthrie HD, Welch GR, Woods LC 3rd. 2013. The effect of freezing rate on the quality of striped bass sperm. Theriogenology 79:940-945. Freking BA, Purdy PH, Spiller SF, Welsh CS, Blackburn HD. 2012. Boar sperm quality in lines of pigs selected for either ovulation rate or uterine capacity. Journal of Animal Science 90(8):2515-2523. Groenen MAM, Archibald AL, Uenishi H, Tuggle CK, Takeuchi Y, Rothschild MF, Rogel-Gaillard C, Park C, Milan D, Megens H-J, Li S, Larkin DM, Kim H, Frantz LAF, Caccamo M, Ahn H, Aken BL, Anselmo A, Anthon C, Auvil L, Badaoui B, Beattie CW, Bendixen C, Berman D, Blecha F, Blomberg J, Bolund L, Bosse M, Botti S, Bujie Z, Bystrom M, Capitanu B, Carvalho-Silva D, Chardon P, Chen C, Cheng R, Choi S-H, Chow W, Clark RC, Clee C, Crooijmans RPMA, Dawson HD, Dehais P, De Sapio F, Dibbits B, Drou N, Du Z-Q, Eversole K, Fadista J, Fairley S, Faraut T, Faulkner GJ, Fowler KE, Fredholm M, Fritz E, Gilbert JGR, Giuffra E, Gorodkin J, Griffin DK, Harrow JL, Hayward A, Howe K, Hu Z-L, Humphray SJ, Hunt T, Hornshøj H, Jeon J-T, Jern P, Jones M, Jurka J, Kanamori H, Kapetanovic R, Kim J, Kim J-H, Kim K-W, Kim T-H, Larson G, Lee K, Lee K-T, Leggett R, Lewin HA, Li Y, Liu W, Loveland JE, Lu Y, Lunney JK, Ma J, Madsen O, Mann K, Matthews L, McLaren S, Morozumi T, Murtaugh MP, Narayan J, Truong Nguyen D, Ni P, Oh S-J, Onteru S, Panitz F, Park E-W, Park H-S, Pascal G, Paudel Y, Perez-Enciso M, Ramirez-Gonzalez R, Reecy JM, Rodriguez-Zas S, Rohrer GA, Rund L, Sang Y, Schachtschneider K, Schraiber JG, Schwartz J, Scobie L, Scott C, Searle S, Servin B, Southey BR, Sperber G, Stadler P, Sweedler JV, Tafer H, Thomsen B, Wali R, Wang J, Wang J, White S, Xu X, Yerle M, Zhang G, Zhang J, Zhang J, Zhao S, Rogers J, Churcher C, Schook LB. 2012. Analyses of pig genomes provide insight into porcine demography and evolution. Nature 491(7424):393-398. Guthrie HD, Welch GR. 2012. Effects of reactive oxygen species on sperm function. Theriogenology 78:1700-1708. Hausman GJ, Barb CR, Lents CA. 2012. Leptin and reproductive function. Biochimie 94:2075-2081. Isom SC, Li R, Whitworth KM, Prather RS. 2012. Timing of first embryonic cleavage is a positive indicator of the in vitro developmental potential of porcine embryos derived from in vitro fertilization, somatic cell nuclear transfer and parthenogenesis. Molecular Reproduction and Development 79:197-207. Kadirvel G, Machado SA, Korneli C, Collins E, Miller P, Bess KN, Aoki K, Tiemeyer M, Bovin N, Miller D. 2012. Porcine sperm bind to specific 6-sialylated biantennary glycans to form the oviduct reservoir. Biololgy of Reproduction DOI:10.1095/biolreprod.112.103879. Klassen H, Kiilgaard JF, Warfvinge K, Samuel MS, Prather RS, Wong F, Petters RM, LaCour M, Young MJ. 2012. Photoreceptor differentiation following transplantation of allogeneic retinal progenitor cells to the dystrophic rhodopsin Pro347Leu transgenic pig. Stem Cells International doi:10.1155/2012/939801. Knox RV, Rodriguez Zas SL, Sloter NL, McNamara KA, Gall TJ, Levis DG, Safranski TJ, Singleton WL. 2013. An analysis of survey data by size of the breeding herd for the reproductive management practices of North American sow farms. Journal of Animal Science 91:433-45. Krisher RL, Prather RS. 2012. A role for the Warburg Effect in preimplantation embryo development: metabolic modification to support rapid proliferation. Molecular Reproduction and Development 79:311-320. Lee K, Redel BK, Teson J, Spate LD, Park K-W, Walters E, Samuel M, Murphy CN, Prather RS. 2013. Piglets produced from cloned blastocysts cultured in vitro with GM-CSF. Molecular Reproduction and Development 80:145-154. Lee K, Wang C, Machaty Z. 2012. STIM1 is required for Ca2+ signaling during mammalian fertilization. Developmental Biology 367:154-162. Lents CA, Barb CR, Hausman GJ. 2013. Chap. 2. Role of adipose secreted factors and kisspeptin in the metabolic control of gonadotropin secretion and puberty. In: Gonadotropin (Vizcarra, J., ed.). InTech, New York. pp. 25-56. Long JA, Conn TL. 2012. Use of phosphatidylcholine to improve the function of turkey semen stored at 4°C for 24 hours. Poultry Science 91:1990-1996. Lovercamp KW, Stewart KR, Xi L, Flowers WL. 2013. Effect of dietary selenium on boar sperm quality. Animal Reproduction Science (in press). Machaty Z, Peippo J, Peter A. 2012. Production and manipulation of bovine embryos: techniques and terminology. Theriogenology 78:937-950. Machaty Z, Wang C, Lee K. 2012. Store-operated Ca2+ entry in germ cells: role in egg activation. In: Store-operated Ca2+ entry (SOCE) pathways; (Eds.: K. Groschner, W.F. Graier, C. Romanin). pp. 461. Springer-Verlag/Wien, Austria. Mack EM, Smith JE, Kurz SG, Wood JR. 2012. Cyclic AMP dependent regulation of ovulatory response genes is amplified by IGF1 due to synergistic effects on Akt phosphorylation and NFkB transcription factors. Reproduction 144:595-602. Mani V, Harris AJ, Keating AF, Weber TE, Dekkers JCM, Gabler NK. 2013. Intestinal integrity, endotoxin transport and detoxification in pigs divergently selected for residual feed intake. Journal of Animal Science (in press). Mao J, Tessanne K, Whitworth KM, Spate LD, Walters EM, Samuel MS, Murphy CN, Tracy L, Zhao J, Prather RS. 2012. Effects of Combined Treatment of MG132 and Scriptaid on Early and Term Development of Porcine Somatic Cell Nuclear Transfer Embryos. Cellular Reprogramming 14:385-389. Mao, J, Whitworth KM, Spate LD, Walters EM, Zhao J, Prather RS. 2012. Regulation of oocyte mitochondria DNA copy number by follicular fluid, EGF, and neuregulin 1 during in vitro maturation affects embryo development in pigs. Theriogenology 78:887-897. McDaneld TG, Smith TPL, Harhay GP, Wiedmann RT. 2012. Next-generation sequencing of the porcine skeletal muscle transcriptome for computational prediction of microRNA gene targets. PloS One 7(7):e42039. McFee RM, Rozell TG, Cupp AS. 2012. The balance of proangiogenic and antiangiogenic VEGFA isoforms regulate follicle development. Cell Tissue Research 349:635-647. McFee RM, Cupp AS. 2013. Vascular contributions to early ovarian development: potential roles of VEGFA isoforms. Reproduction, Fertility and Development 25:333-342. Men H, Walters EM, Nagashima H, Prather RS. 2012. Emerging applications of sperm, embryo and somatic cell cryopreservation in maintenance, relocation and rederivation of swine genetics. Theriogenology 78:1720-1729. Mesa H, Cammack KM, Safranski TJ, Green JA, Lamberson WR. 2012. Selection for placental efficiency in swine: Conceptus development. Journal of Animal Science 90:4217-22. Miao Y-L, Zhang X, Zhao J-G, Spate L, Zhao M-T, Murphy CN, Prather RS, Sun Q-Y, Schatten H. 2012. Effects of griseofulvin on in vitro porcine oocyte maturation and embryo development. Environmental and Molecular Mutagenesis 53:561-566. Miles EL, OGorman C, Zhao J, Samuel M, Walters E, Yi Y-J, Sutovsky M, Prather RS, Wells KD, Sutovsky P. 2013. Transgenic Pig Carrying Green Fluorescent Proteasomes. Proc. Natl. Acad. Sci. USA 110:6334-6339. Miles EL, Sutovsky P. 2013. Sperm proteasome as a putative egg coat lysin in mammals. In: Sexual Reproduction in Animals and Plants. Hitoshi Sawada, Naokazu Inoue, and Megumi Iwano, Editors. Springer (in press). Miles JR, Vallet JL, Ford JJ, Freking BA, Cushman RA, Oliver WT, Rempel LA. 2012. Contributions of the maternal uterine environment and piglet genotype on weaning survivability potential: I. Development of neonatal piglets after reciprocal embryo transfers between Meishan and White crossbred gilts. Journal of Animal Science 90:2181-2192. Miller D, Rathbun T, Boyle D, Troyer D, Davis DL. 2012. Confocal imaging of trans-epithelial trafficking by immune and umbilical cord stem cells in the neonatal porcine intestine. Anatomia Histologia Embryologia 41:461-468. Mitchell AD, Ramsay TG, Caperna TJ, Scholz AM. 2012. Body composition of piglets exhibiting different growth rates. Archiv Tierzucht 55:356-363. Mousel EM, Cushman RA, Perry GA, Kill LK. 2012. Effect of heifer calving date on longevity and lifetime productivity. Applied Reproductive Strategies in Beef Cattle (Proc., Sioux Falls, SD; 3-4 Dec 2012):23-31. Nagyova E, Scsukova S, Nemcova L, Mlynarcikova A., Yi Y-J, Sutovsky M, Sutovsky P. 2012. Inhibition of proteasomal proteolysis affects expression of extracellular matrix components and steroidogenesis in porcine oocyte-cumulus complexes. Domestic Animal Endocrinology 42:50-62. Newcomer SC, Taheripour P, Bahls M, Sheldon R, Foust KB, Bidwell CB, Cabot RA. 2012. Impact of porcine maternal exercise training during pregnancy on endothelial call function of offspring at birth. Journal of Developmental Origins of Health and Disease 3:4-9. Nickelson KJ, Stromsdorfer KL, Pickering RT, Liu TW, Ortinau LC, Keating AF, Perfield JW. 2013. A comparison of inflammatory and oxidative stress markers in adipose tissue from weight-matched obese male and female mice. Experimental Diabetes Research (in press). Nonneman DJ, Brown-Brandl TM, Jones SA, Wiedmann RT, Rohrer GA. 2012. A defect in dystrophin causes a novel porcine stress syndrome. BMC Genomics 13:233. Ostrup O, Olbricht G, Ostrup E, Hyttel P, Collas P, Cabot R. 2013. RNA profiles of porcine embryos during genome activation reveal complex metabolic switch sensitive to in vitro conditions. PLoS One (in press). Park KE, Inerowicz HD, Wang X, Li Y, Koser S, Cabot RA. 2012. Identification of Karyopherin ±1 and ±7 Interacting Proteins in Porcine Tissue. PLoS One 7:e38990. Park KE, Johnson CM, Cabot RA. 2012. BIX-01294, an inhibitor of the histone methyltransferase EHMT2, disrupts H3K9 dimethylation in the cleavage stage porcine embryo. Reproduction, Fertility and Development 24:813-821. Perry GA, Larimore EL, Bridges GA, Cushman RA. 2012. Management strategies for improving lifetime reproductive success in beef heifers. Applied Reproductive Strategies in Beef Cattle (Proc., Sioux Falls, SD; 3-4 Dec 2012):249-266. Prather RS, Lorson M., Ross JW, Whyte JJ, Walters E. 2012. Genetically Engineered Pig Models for Human Diseases. Annual Reviews in Animal Biosciences, Palo Alto, CA. Prather RS. 2013. Pig genomics for biomedicine. News & Views. Nature Biotechnology 7;31(2):122-124. Prather RS, Lorson M, Ross JW, Whyte J, Walters E. 2013. Genetically engineered pig models for human diseases. In: Annual Review of Animal Biosciences, Vol. 1 (L. Andersson, D.E. Bauman, W.C. Brown, H.A. Lewin, S.J. OBrien, E.A. Ostrander, R.M. Roberts, L.J. Saif, G.E. Seidel, Jr., A. Xu, Eds.). Annual Reviews, Palo Alto, CA. Ramsay TG, Stoll MJ, Conde-Aguilera JA, Caperna TJ. 2013. Peripheral tumor necrosis factor ± regulation of adipose tissue metabolism and adipokine gene expression in neonatal pigs. Vet Res Commun. 37:1-10. Redel BK, Brown AN, Spate LD, Whitworth KM, Green JA, Prather RS. 2012. Glycolysis in preimplantation development is partially controlled by the Warburg Effect. Molecular Reproduction and Development 79:262-271. Riley DG, Chase CC, Coleman SW, Phillips WA, Miller MF, Brooks JC, Johnson DD, Olson TA. 2012. Genetic effects on carcass quantity, quality, and palatability traits in straightbred and crossbred Romosinuano steers. Journal of Animal Science 90:2159-2166. Ringwelski JM, Beever JE, Knox RV. 2013. Effect of interval between inseminations when using frozen-thawed boar sperm on fertility and fetal paternity in mature gilts. Animal Reproductive Science 137: 197-204. Rohrer GA, Nonneman DJ, Miller RK, Zerby H, Moeller SJ. 2012. Association of single nucleotide polymorphism (SNP) markers in candidate genes and QTL regions with pork quality in commercial pigs. Meat Science 92(4):511-518. Ross JW, Castro JF, Zhao J, Samuel M, Walters E, Rios C, Bray-Ward P, Jones BW, Marc RE, Wang W, Zhou L, Noel JM, McCall M, DeMarco P, Prather RS, Kaplan HJ. 2012. Generation of an inbred miniature pig model of retinitis pigmentosa. Investigative Ophthalmology & Visual Sciences 31:501-507. Ross JW, Keating AF. 2012. Mechanisms of ovarian atresia induced by xenobiotic exposures. In: Gynecology. Ed. A. Darwish. InTech Publishing, pp 77-96. Runcie DE, Wiedmann RT, Archie EA, Altmann J, Wray GA, Alberts SC, Tung, J. 2013. Social environment influences the relationship between genotype and gene expression in wild baboons. Philosophical Transactions of the Royal Society B: Biological Sciences 368(1618): 20120345. 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