Brief Summary of Minutes of Annual Meeting

Pat Donald called the meeting to order and welcomed participants at 8:30 am at the Savannah Marriott Center in conjunction with the Society of Nematologists 51st Annual Meeting. Dr. Steve Pueppke, Administrative Advisor, welcomed the members and provided an outline of potential changes to future research funding and announced the next mid-term report for NC1197 will be due in 2013. Following Dr. Pueppkes presentation, participants proceeded with individual state research reports pertaining to the NC1197 project objectives and concluded with the annual Business Meeting. The general consensus for the 2013 meeting was that it would be in conjunction with the SON 52nd annual meeting in Cleveland Ohio. The primary purpose of the meeting was to report and discuss recent research results pertaining to the new project  Practical Management of Nematodes on Corn, Soybean, and Other Crops of Regional Importance - initiated in 2011. Objective 1: Develop, evaluate, improve, and integrate management techniques for plant-parasitic nematodes in the North Central Region to increase grower profitability. Evaluation of SCN-resistant soybean lines and cultivars: Participating States (Iowa, Tennessee, Michigan, Indiana, Arkansas, Wisconsin, Minnesota and Ontario) reported on the evaluation of SCN resistant lines and cultivars. Hundreds of soybean cultivars for resistance to SCN in field and greenhouse trials were evaluated for resistance to the most common state HG Types and for agronomic performance. Results were published online (see publications) and distributed at numerous field days and extension meetings. In addition to SCN testing, all soybeans entered into the Arkansas Variety Testing Program are greenhouse tested for resistance to SCN, Southern Root-knot Nematode (SRKN), and Reniform Nematode (RN). Arkansas and the boot-heel of Missouri are the only states with participants in this committee where SRKN and RN are a problem on soybean. All varieties are tested for SCN and RKN each year and all varieties that were not previously tested are evaluated for RN resistance. Assessment of HG Types and other aspects of virulence: Minnesota field plot experiments were initiated in 2003 to study the effect of tillage and source of resistance on SCN population density and virulence phenotype. Tillage had little effect on SCN virulence phenotype and population density. Among the three SCN-resistant cultivars, the PI 88788-derived cultivar supported the greatest SCN egg population density, the Peking-derived cultivar was intermediate, and the PI 437654-derived cultivar supported the smallest egg population density. No clear pattern of effect of rotations of the three sources of resistance on virulence phenotype was observed during the 8 years of study. Wisconsin HG Type survey results continue to indicate a trend in the geographic distribution of HG types, with more HG 1- populations located in the East Central and Northeast regions of the state. In Arkansas SCN race test are used instead of HG types for a variety of reasons. In 2012 races 2, 3, 5, 8 and 14 were tested. In the last 3 years only races 2 and 5 were found in samples submitted to the Arkansas Nematode Assay program. In the last statewide survey they were the predominant races found. Iowa performed HG type tests on SCN populations from nine state varietal experimental test locations. Results are used to assess the effects of the virulence phenotype of the SCN population (the HG type) on agronomic performance of the soybean varieties. In Ontario HG Type testing was completed on 31 field samples in 2010-11. HG Type results indicate 2011 samples had as much reproduction on HG 1 when compared to HG 2 (PI 88788). Ontario SCN populations appear to be adapting to Peking resistance in the absence of being challenged to the very few historically planted Peking resistant varieties. HG 4 (PI 437654) has complete resistance not overcome by SCN populations in Ontario. Survey results from 2006-11 indicated that Ontario had approximately 25 different combinations of HG Types. The unique diversity of HG Types contrasted with less diverse populations found in Tennessee, Indiana and Illinois with a longer SCN infestation history. Evaluation of new nematicidal seed treatments for management of SCN and corn-parasitic nematodes: Exploratory work is being conducted by several members of the group (Illinois, Indiana, Iowa, Wisconsin, Ontario, and Tennessee) on nematicide seed treatments that are in the development stage or have been recently marketed by several private companies. Five different large plot experiments were conducted at Iowa State University research farms in northwest, northeast, central, southwest, and southeast Iowa in 2011 to determine the effects of the seed treatment protectants Avicta from Syngenta Seedcare and Votivo from Bayer CropScience on population densities of plant-parasitic nematodes on corn and on corn yields. In 2011 experiments, there were significant differences in nematode population densities at the V5/V6 soil sampling date among the treatments in only two experiments and no significant differences in corn yields among the treatments in any of the experiments. Seed treatments were established in north and mid-state in Indiana to document the effect of these treatments on population density of SCN and yield of soybean. No dramatic effect was observed from any of these treatments. In addition, Indiana continued to evaluate experimental seed-treatment products from various chemical companies against SCN in the field. No significant reduction in population density of SCN or increases in soybean yield were found. Minnesota soil seed treatments were evaluated in the greenhouse in field soil and autoclaved field soil for their effect on SCN egg population density and plant growth. No seed treatment effect on SCN egg population density and plant growth was observed. Additional outreach support projects for the suppression of SCN were carried out in joint collaboration with Ontario Ministry of Agriculture and Agriculture Canada on several farm trials indicating inconclusive results. SCN micro-plot field projects results with oriental processed mustard seed hulls indicated no differences between non-treated plots as compared to 6 rates of mustard hulls. Evaluation of rotational crops and cultural practices for SCN and corn-parasitic nematode management: Michigan reported on a long term rotational study on how SCN adapts and increases to damaging levels when introduced into SCN-virgin land and subjected to agronomic practices. Over the course of six years (2003 2008), SCN population density, plant stand, and yield were measured on susceptible and resistant soybeans, corn and rotation combinations. In all cases, the population density of SCN was highest in susceptible and lowest in corn or corn rotations both on tilled and no-till plots. In 2007, in both tillage systems, and in 2008, in no-till plots, yield of SCN-infested plots was significantly lower than non-infested plots. The impact of this study provides agro-biologically based timeline information that is critical for SCN management. Effects of long-term corn-soybean rotation and nematicide Counter on SCN and other plant-parasitic nematodes, and soybean and corn yields were evaluated in Minnesota for 2010-11. While crop sequence significantly affected SCN, Pratylenchus, and Helicotylenchus, the nematicide treatment did not affect the plant-parasitic nematodes at planting, midseason and harvest except that Helicotylenchus population density at harvest was reduced by the nematicide. Investigation of pest interactions involving plant-parasitic nematodes and their contribution to yield losses in North Central Region crops: Indiana Soybean Association funded a collaborative research project in Indiana to study the SCN-SDS relationship. Five soybean cultivars were planted, four with various sources of SCN and SDS resistance. Even though no obvious symptoms of SDS were observed in one of the two state locations, the same dramatic yield response from varieties with the Peking source of resistance was observed as where the disease was visually present. Objective 2: Determine the relationships among nematode population characteristics, crop injury, and soil health. Develop a list of damaging nematodes for corn and other major crops in the North Central Region: In Ontario, parasitic nematodes of corn were surveyed in co-operation with project coordinator, T. Jackson (Nebraska), as part of the NC corn survey project. Samples of soil and plants were collected by the Ontario Ministry of Agriculture and Food in collaboration with the NC project. Random samples were collected from 235 fields and 28 % had damaging levels of the most common parasitic nematodes found on corn. Root lesion (Pratylenchus spp) was present in 37% of the corn root samples. Determine damage thresholds for major corn-parasitic nematode species: Indiana had a two-fold increase in the number of corn samples received in our Nematology Laboratory, as compared to 2010. Most of these samples had above-thresholds levels of needle, lance and lesion nematodes. Needle nematode on crabgrass roots was found late in the fall in southern Indiana. Five large on-farm corn plots were established in southern and northern Indiana to evaluate the effectiveness of available seed treatments on corn parasitic nematodes and on corn yields. Results of these studies were presented as a poster at the 2012 SON meeting. Characterize infra-specific variation in host-parasite interactions across the North Central Region: Michigan reported on relationships among SCN and nematode community structures as investigated in 2008 and 2009. The results indicate that SCN population had positive correlations with total nematode abundance, total non-SCN nematode abundance, free-living nematode abundance, and bacteria-feeding nematode abundance in both years. This suggests that conditions favoring free-living nematodes can also favor SCN. Further analysis between nematode populations and food web and various management strategies (tillage, cultivars, rotations) suggests that in addition to direct impacts, tillage and susceptible cultivar may have indirect impacts on increasing SCN population density through favoring free-living nematodes, while crop rotation is likely to have an indirect impact in decreasing SCN population density through decreasing free-living nematodes. Develop predictive models of nematode population dynamics for SCN and other regionally-important plant-parasitic nematodes : A soil survey, funded by the United Soybean Board in 2011, was initiated to determine the distribution of plant parasitic nematodes in soybean production fields in southern soybean production fields in 11 states. In Tennessee 78 soil samples were collected at random in west and central Tennessee. In the samples collected, 82% had plant-parasitic nematodes. Plant parasitic nematodes recovered included Heterodera glycines (16%), Meloidogyne incognita (4%), Pratylenchus sp. (19%), Helicotylenchus (50%), Tylenchorhynchus sensu lato (11%), Rotylenchulus (3%). A low level of Hoplolaimus magnistylus was detected in soybean, corn and cotton fields. The survey is in the second year and targeted high production acreage and counties in middle and east Tennessee. For the fourth year, Wisconsin collected soil samples from 24 random soybean sites within 2 fields at growth stage VE-VC and related nematode counts to yield. For the third year, there was a significant relationship between population densities of P. penetrans at planting and soybean yield. Objective 3. Develop tools for technology transfer for management of regionally important nematodes with special reference to soybean cyst nematode and corn parasitic nematodes. Assemble a dynamic database of soybean cultivar characteristics related to SCN resistance: Most NC1197 participants (Iowa, Tennessee, Michigan, Indiana, Wisconsin, Arkansas, Minnesota and Ontario) reported on the evaluation of SCN resistant lines and cultivars. Hundreds of soybean cultivars were tested in the field and greenhouse trials and were evaluated for resistance to the most common state/province HG Types and for agronomic performance. Results were published in hardcover and online (see publications) and distributed at numerous field days and extension meetings in each state/province. Provide readily-accessible and reliable information on rapidly evolving nematode management strategies such as the new commercial seed treatments for nematode control: The committee decided that from a technical perspective it should provide a review and update of the listing of the most common NC parasitic nematodes on corn. It also agreed to provide reviews of damaging threshold levels as required. An updated SCN Management Guide based on recent research data was published and distributed in Minnesota. Wisconsin began using the total nematode risk index for samples submitted to their diagnostic service. A risk score was assigned to clinic samples submitted from corn fields from March to June 30th for the period 1999-2012. There was a significant increase in the risk scores over the 14-year period. It appears that the change is due primarily to an increase in Pratylenchus spp. Clinic samples are a biased data set and have limitations but offer great opportunity for conveying the impact of nematodes in the North Central region. PLEASE NOTE: State reports are attached as part of the meeting minutes.

1. Hundreds of germplasm lines and commercially marketed soybean cultivars were evaluated independently for reaction to multiple soybean cyst nematode populations. This information was made available to producers electronically as well as in print form. 2. Several studies were initiated on plant-parasitic nematodes parasitizing corn roots, seed treatment studies on both corn and soybean and other management strategies for plant-parasitic nematodes. 3. New extension guides containing information on management of economically important plant-parasitic nematodes in the region were published.

Journal Articles Campbell, J.A., Goheen, S.C. and Donald, P. 2011. Extraction and Analysis of Inositols and Other Carbohydrates from Soybean Plant Tissues. Pages 421 446. in Recent Trends for Enhancing the Diversity and Quality of Soybean Products, D. Krezhova ed. InTech publisher http://www.intechopen.com/articles/show/title/extraction-and-analysis-of-inositols-and-other-carbohydrates-from-soybean-plant-tissues Donald, P.A., Heinz, R., Bernard, E., Hershman, D., Hensley, D., Flint-Garcia, S., and Joost, R. 2012. Distribution, host status and potential sources of resistance to Vittatidera zeaphila. Nematropica 42: 91-95. McDonald IV, L. W., Goheen, S. C., Donald, P. A., and Campbell J. A. 2012. Identification and quantitation of various inositols and o-methylinositols present in plant roots related to soybean cyst nematode host status. Nematropica 42:1-8. Melakeberhan, H., Douches, D. and Wang, W. 2012. Interactions of Selected Potato Cultivars and Populations of Meloidogyne hapla Adapted to the US Midwest Soils. Crop Science, 52:1-6. Melakeberhan, H. and Wang, W. 2012. Suitability of celery cultivars to populations of Meloidogyne hapla. Nematology, 14:623-629. Melakeberhan, H. and Wang, W. 2012. Proof-of-concept for managing Meloidogyne hapla parasitic variability in carrot production soils. Nematology, 14:623-629. Robbins, R. T., E. Shipe, P. Arelli, G. Shannon, K. M. Rainey, P. Chen, Stella K. Kantartzi, L. E. Jackson, E. E. Gbur, D. G. Dombek, & J. T. Velie. 2012. Reniform nematode reproduction on soybean cultivars and breeding lines in 2011. Proceeding of the 2011 Beltwide Cotton Conferences, Atlanta, Georgia, January 4-7, 2011, Pgs. 167-174. Tylka, G.L., A.J. Sisson, L.C. Jesse, J. Kennicker and C.C. Marett. 2011. Testing for plant-parasitic nematodes that feed on corn in Iowa 2000-2010. Online. Plant Health Progress doi:10.1094/PHP-2011-1205-01-RS. Tylka, G.L., T.C. Todd, T.L. Niblack, A.E. MacGuidwin, and T. Jackson. 2011. Sampling for plant-parasitic nematodes in corn strip trials comparing nematode management products. Plant Health Progress doi:10.1094/PHP-2011-0901-01-DG. Xia Yan, Xin-Yue Cheng, Yun-Sheng Wang, Ji Luo, Zhen-Chuan Mao, Virginia R. Ferris, Bing-Yan Xie. Comparative transcriptomics of two pathogenic pinewood nematodes yields insights into parasitic adaptation to life on pine hosts. 2012. doi:10.1016/J.gene.2012.05.041 Abstracts Diaz-Arias, M., G.L. Tylka, L. Leandro, and G.P. Munkvold. 2011. Interactions between Fusarium root rot pathogens and Heterodera glycines, on soybean roots. Phytopathology 101:S42. Faghihi, J, R. A. Vierling and V. R. Ferris. 2010. Effect of fall annual rye grass seeding on soybean cyst nematodes. Journal of Nematology 42:242. Hong, S. C., A. MacGuidwin, and C. Gratton. . 2011. Soybean aphid (Aphis glycines Matsumura) and soybean cyst nematode (Heterodera glycines Ichinohe) interactions in the field and effects on soybean yield. Journal of Economic Entomology 104:1568-1574. MacGuidwin, A. E. 2011. Is it nematode or Verticillium that causes Mr. Potato to die early? Phytopathology 101:S209. MacGuidwin, A. E. 2011. Damage potential of Pratylenchus penetrans to soybean. Journal of Nematology 43:259-260. Niblack, T.L., J. Bond, and G.L. Tylka. 2011. The nightmare of plant diseases associated with soybean cyst nematodes. Phytopathology 101:S209. R. T. Robbins, E. Shipe, P. Arelli, G. Shannon, K. M. Rainey, P. Chen, S. K. Kantartzi, L. E. Jackson, E. E. Gbur, D. G. Dombek, and J. T. Velie. 2012. Reniform nematode reproduction on soybean cultivars and breeding lines in 2011. Program of the 2012 Beltwide Cotton Conferences, Orlando. Robbins, R. T., L. E. Jackson, K. Rowe, D. Crippen and T. Kirkpatrick. 2012. Soybean Varieties With Reniform and Root-Knot Nematode Resistance, 1998 2011. Abstracts, 39th Annual Meeting, Southern Soybean Disease Workers, March 7-8, 2012 Pensacola, FL. p. 27 Tatalovic, N., G.L. Tylka, and L.F. Leandro. 2011. Microscopic observation of the interaction between the soybean sudden death syndrome pathogen, Fusarium virguliforme, and the soybean cyst nematode, Heterodera glycines, in soybean roots. Phytopathology 101:S175. Welacky, Tom W, 2011. Investigations of legume resistance reactions to Heterodera glycines in Ontario fields. Journal of Nematology. Wiggs, S.N. and G.L. Tylka. 2011. The nature of the relationship between soybean cyst nematode population densities and soil pH. Phytopathology 101:S191. Research Reports: Allen, F.L., Johnson, R., Williams, R.C., Jr, McClure, A.T., Newman, M., Donald, P. Soybean Variety Performance Tests in Tennessee. 2011. http://varietytrials.tennessee.edu/pdffiles/2011trialdata/soybean_FINALrpt.pdf. Research Report 12-03. University of Tennessee Institute of Agriculture (Technical Bulletin). Faghihi, J., Krupke, C., and V. Ferris. Nematode Update: Needle Nematodes on Wheat - Pests and Crops Newsletter 4/8/2011. Faghihi, J. Drought season highlights damage caused by nematodes Ag Answers 7/3/2012 Welacky, T.W. Annual Reports - Ontario Soybean Variety Trials. Ontario Oil and Protein Seed Crop Committee. 12p. Soybean Data Co-coordinator of publication. Producer and editor of internet site, http://www.gosoy.ca including Variety Information and Performance Profile (ViPP), performance graphs, % Protein and % Oil reports and tables of varietal responses to SCN and various diseases in Ontario. Extension Publications: Chen, S. (ed). 2011. Soybean Cyst Nematode Management Guide. University of Minnesota Extension. Mueller, D., A. Sisson, E. Hodgson, A. Mallarino, C. McGrath, M. ONeal, P. Pedersen, C. Pilcher, R. Pope, M. Rice, A. Robertson, J. Sawyer, K. Schaefer, K. Simon, G. Tylka, and D. Wright. 2011. Soybean field guide, 2nd Edition. Iowa State University Extension Publication CSI 010, 68 pp. Tylka, G.L. and M. P. Mullaney. 2011. Soybean cyst nematode-resistant soybeans for Iowa. Iowa State University Extension Publication PM 1649, 27 pp. Tylka, G.L., G.D. Gebhart, C.C. Marett, M.P. Mullaney, and S.N. Wiggs. 2011. Evaluation of soybean varieties resistant to soybean cyst nematode in Iowa 2011. Iowa State University Extension, publication IPM 52. Integrated Crop Management News articles (online at www.extension.iastate.edu/cropnews/) Tylka, G. 2011. Sampling for nematodes that feed on corn this season. Iowa State University Integrated Crop Management News (5 June 2011). Tylka, G. 2011. SCN females now apparent on soybean roots. Iowa State University Integrated Crop Management News (21 June 2011). Tylka, G. 2011. Corn or soybeans looking stressed? Nematode damage is becoming apparent. Iowa State University Integrated Crop Management News (11 July 2011). Robertson, A., D. Mueller, L. Leandro, G. Tylka, and XB Yang. 2011. Begin scouting for sudden death syndrome in soybean. Iowa State University Integrated Crop Management News (20 July 2011). Tylka, G. 2011. Comparing nematode management products on corn in strip trials. Iowa State University Integrated Crop Management News (9 September 2011). Tylka, G. 2011. Know your choices: hundreds of SCN-resistant soybean varieties for Iowa. Iowa State University Integrated Crop Management News (21 October 2011). Tylka, G. 2011. Fall time to sample for SCN not for nematodes that feed on corn. Iowa State University Integrated Crop Management News (11 November 2011). Tylka, G. 2011. A 10-year summary of testing for nematodes that feed on corn in Iowa. Iowa State University Integrated Crop Management News (7 December 2011).