Brief Summary of Minutes of Annual Meeting

Brief Summary of Minutes of Annual Meeting: The meeting convened at 9:30 a.m. on Monday, April 30, 2012 Chair: Jeff Brecht Vice Chair: Charles Forney Secretary: Eva Almenar List of Attendees: Name Institution email Participants: Eva Almenar Michigan State University ealmenar@msu.edu Jeff Brecht University of Florida jkbrecht@ufl.edu Marita Cantwell UC Davis micantwell@ucdavis.edu Lihua Fan Ag. & Agri-Food Canada, Kentville Lihua.fan@agr.gc.ca Charles Forney Ag. & Agri-Food Canada, Kentville charles.forney@agr.gc.ca Mosbah Kushad University of Illinois kushad@illinois.edu Mikal Saltveit UC Davis mesaltveit@ucdavis.edu Angel Shaw Iowa State University amgelaml@iastate.edu Peter Toivonen Ag. & Agri-Food Canada, Summerland peter.toivonen@agr.gc.ca Yanyun Zhao Oregon State University yanyun.zhao@orgonstate.edu Others: Marina Buccheri Agricultural Research Council, Italy marina.buccheri@entecra.it Giancarlo Colelli University of Foggia, Italy g.colelli@unifg.it Reuben Moore Mississippi State University reubenm@mafes.msstate.edu Ram Rao NIFA, USDA rrao@nifa.usda.gov The meeting was convened with a welcome by Jeff Brecht and a round of introductions. 1. Review of agenda  Additions to the agenda: (1) Current funding limitations being a problem for scientist to attend S-294 meetings (several members), (2) Development of an ISHS working group in fresh-cut produce in support of the ISHS meeting that will be held in Davis in 2015 (Dr. Marita Cantwell), (3) Announcement of a conference focused on MAP that will be held in Italy next year (Dr. Giancarlo Colelli), (4) Announcement of open postdoc positions (Drs. Giancarlo Colelli and Eva Almenar). The agenda of the 2012 meeting was approved by voice vote. 2. Approval of 2011 minutes  2011 minutes were approved unanimously. 3. Report from the Administrative Adviser, Reuben Moore  Dr. Reuben Moore stressed the importance of the S-294 project, which is the most productive project of the several projects he advises. He proposed to apply to the 2012 Experimental Station Section Award for Excellence in Multistate Research and gave to the secretary the related paperwork. Dr. Reuben Moore complemented Dr. Eva Almenar for the good job as a secretary. He also discussed our current partnership with the UnitedFresh and the importance of this being maintained due to the mutual benefits (they give us a form to present our research (posters) and in return, we present our cutting research to industry). Dr. Reuben Moore provided an update on funding programs like land-grand funding from HASS funds. These are not competitive and require one to spend 25% of the funding to get new funding. He suggested this could be funding opportunities for individuals who want to attend the multistate project or for collaborations with others. 4. Remarks and presentation about SCRI from NIFA Representative, Ram Rao  Dr. Ram Rao provided an update on the funding programs in the Specialty Crops Research Initiative (SCRI): (1) Food safety-SCRI funding increases from $5 to $19 millions, (2) Quality-SCRI funding is dispersed among all areas and therefore, it is difficult to specify a dollar amount, (3) Funding is available for metrics since metrics are needed to implement safety regulations, (4) NIFA representatives would like to receive feedback about the RFP from the S-294 members, and (5) Increase of virtual panels due to the serious cuts in travel funds. Dr. Reuben Moore expressed his concern about the integrity of the panels. Additional information on funding opportunities: (1) AFRI: funding for detection of pathogens increased by $6 millions, AFRI Foundational program will combine 2012 and 2013 RFP (August 15), and funding for AFRI safety challenge is expected to be increased from $254 to $325 millions if accepted by the President, (2) NIFSI: no funding for the following years, (3) OREI: Dr. Ram Rao asked us to share our thoughts as a group about evaluation panels and comments on the RFP, and (4) Improved processing technology: fruits and vegetables are included in the RFP. Dr. Ram Rao indicated that smaller amounts of funding than those of previous years will be assigned to future projects as multi-institution collaborations will not be requested. 5. Science Symposium  The science symposium organized by S-294 members, which has traditionally been well attended in past meetings, was broadly discussed. Dr. Ram Rao informed us that funding opportunities to support a science symposium organized by S-294 members are available at symposium proposal of any program. Dr. Ram Rao suggested the programs Improved Processing Technology and Food Safety. He also suggested to prepare the symposium and to link it to United Fresh (possible speaker cost sharing between grant and United Fresh). He also proposed to join other multistate groups in order to strengthen the proposal. A discussion on topics for the science symposium followed Dr. Ram Raos comments and suggestions. Suggested topics for the symposium were as follows: (1) Identification of gaps in science and knowledge, (2) Identification of issues related to the fresh-cut industry, (3) Development of models to determine shelf life, (4) Procedures and metrics for safe fresh-cut produce, and (5) Focus on small scale markets: local markets or grocery stores or farmer markets, (6) Cutting edge processing technology, (7) Symposium related to cantaloupe, (8) Symposium related to soft and rugose surfaces, (8) Identification of issues related to fresh-cut apples, (9) Emerging fresh-cut produces, (10) Tropical produce, and (11) No risk but answers related to pathogens. It was suggested to publish the proceedings and it could be done in the form of metrics (recommendations/guidelines). It was also suggested to have ISHS and S-294 as a sponsor and co-sponsor and to put together a proposal for 2015 SCRI Special Grants-Planning Grants. A workshop is proposed instead of a symposium. It is proposed that a working group could bring up the topic to Dr. David Gombas (industry could support the workshop). 6. Election of new secretary  Dr. Susan Bach was nominated and unanimously elected as Secretary for 2012-2013 (Dr. Peter Toivonens motion, Dr. Jeff Brecht moved, and Dr. Mikal Saltveit seconded). 7. Submission of book authored by the S-294 members  Dr. Peter Toivonen informed us that he was contacted by Wiley-Blackwell about submitting a book proposal. He suggested focusing on fresh-cut fruits and vegetables and having the book authored by the S294, and Wiley-Blackwell was very interested. The follow-up discussion to Dr. Peter Toivonens comment was as follows: (1) the book should focus on industry and therefore, this should have a more applied stand point, (2) the book could have an extension point of view, (3) suggested topics to be included in the book were: raw material quality, shelf life expectations, packaging in the practical way, best manufacturing practices, and good agricultural practices, (4) international collaboration was proposed and unanimously desired, (5) close collaboration with industry for topics to be included was agreed, (6) electronic or hard copy format was discussed without a final agreement, (7) economic support for member travel from industry was suggested, (8) Drs. Peter Toivonen, Jeff Brecht, Marita Cantwell, and Eva Almenar agreed to move the idea forward and target publication data was set for May 2013. 8. Station reports  Details of these reports are available in the annual reports posted at http://postharvest.ucdavis.edu/research/ 9. New business Funding limitations to attend S-294 meetings  Current funding limitations being a problem for scientist to attend S-294 meetings were also discussed. S-294 website  Dr. Marita Cantwell proposed to maintain the S-294 website linked to the UC Davis one (http://postharvest.ucdavis.edu/research/). Dr. Reuben Moore clarified that S-294 members do not have access to the S-294 website postings but the S-294 members have to the minutes. Dr. Marita Cantwell informed that she will be the contact person in Davis responsible for the S-294 website and that a secretary will feed information there. USDA Handbook 66  Dr. Mikal Saltveit reported that Handbook 66 has been revised and is ready to be published. Mike also informed us that Hanbook 66 could be posted on either USDA website or UC Davis website, and that there will be a mechanism to get feedback and accessibility to chapter revision. ISHS working group in fresh-cut produce in support of the ISHS meeting  Development of an ISHS working group in fresh-cut produce in support of the ISHS meeting that will be held in Davis in 2015 (cosponsoring and review of papers) (Dr. Marita Cantwells motion, Mikal Saltveit moved, and Dr. Peter Toivonen seconded), Announcement of a conference focused on MAP  Announcement of a conference focused on MAP that will be held in Italy next year (Dr. Giancarlo Colelli). Announcement of open postdoc positions  Announcement of open postdoc positions (Drs. Giancarlo Colelli and Eva Almenar). We thanked to UnitedFresh Produce Association for providing food and beverage service and a room for the meeting. Meeting adjourned at 5:00 pm

Accomplishments: Objective 1. Evaluate methods of sampling and measuring flavor and nutrition of fresh-cut products to facilitate comparison to traditional shelf life factors. " Instrumental and sensory evaluation on a number of different mango cultivars, both US grown and imported, is being conducted to assist the mango industry with harvest timing for optimal fresh-cut quality (Barrett UCD CA). " The use of impedance spectroscopy for following shelf life of fresh-cut mangoes, melons and potatoes has been evaluated (Barrett UCD CA). " Continuation of experienced consumer or trained descriptive panels and chemical analysis (volatiles, sugars, acids) to evaluate tangerine, strawberry, and tomato germplasms from University of Florida. 10-years of tomato data are being analyzed to establish a model for tomato quality (Baldwin, Plotto, Narciso and Bai USDA/ARS USCSPL FL). " A two-year texture profile of fresh blueberries was completed with UF blueberry breeder; data will be used to understand inheritance of the firmness trait in blueberries (Baldwin, Plotto, Narciso and Bai USDA/ARS USCSPL FL). " A new experiment was designed to understand the inheritability of the aroma volatile methylanthranilate (MA) in strawberries. MA imparts fruity flavor to strawberries and is only produced in European lines (Baldwin, Plotto, Narciso and Bai USDA/ARS USCSPL FL). " A sensory study with interactions of limonin and nomilin, two bitter compounds in oranges harvested from trees having Huanglongbing (citrus greening) disease has been completed, and their effect on orange and orange juice flavor have been studied (Baldwin, Plotto, Narciso and Bai USDA/ARS USCSPL FL). " Loss of soluble phenolics due to chemical decomposition during digestion has been demonstrated in studies looking at in vitro digestion of apple varieties (Toivonen, Bach, and Delaquis AAFC Summerland). Objective 2. Develop new strategies to improve and better maintain inherent fresh-cut product quality and nutrition. " The effects of immersing whole Serrano peppers in PRO-SAN (an EPA-approved vegetable wash) were investigated. Peppers were immersed (for 2.0 or 4.0 minutes) in water (control), chlorine (CL; 150 ppm; adjusted to pH 6.4), PRO-SAN, PRO-SAN-LC, PRO-SAN-soft, and PRO-SAN-PF at concentrations (0.1 to 1.0%) stated by the manufacturer. After treatment peppers were stored for 7 days at room temperature. PRO-SAN and its derivatives did not adversely affect the quality parameters of peppers evaluated during 7 days of storage (Mendonca, Boylston and Wilson ISU IA). " The efficacy of various solutions of Pro-San" (Microcide, Inc®, Detroit, MI) was tested on romaine lettuce shelf life. Pro-San" LC and Pro-San" LC Soft were tested at two dilutions, 0.78% and 0.19%, at various time points and compared to a dry control and 150 ppm bleach solution, which is the currently used industry standard. Results indicated there were no quality effects from any treatment group over the seven days. The 0.78% Pro-San" and Pro-San" Soft solutions did not cause a sensory quality change of the romaine lettuce when rinsed off before consumption, according to the instrumental measurements of color and the overall difference test completed by the sensory panel (Mendonca, Boylston and Wilson ISU IA). " A study was conducted to determine respiration rate and gas permeance of pears influenced by cultivar and storage temperature. After satisfying the chill requirement, Starkrimson, Bartlett, Bosc, Comice, and dAnjou exhibited respiration rates from 6.9 to 1.1 µmol kg-1 h-1 at 30 °F, and 35.8 to 8.6 µmol kg-1 h-1 at 68 °F, respectively. After satisfying the chill requirement plus 7 d at 68 °F, different cultivars had internal O2 and CO2 from 17.1 and 3.8% to 19.2 and 1.3%, respectively. Susceptibility to CO2/O2 injury was assessed by treating each cultivar with a Carnuaba-based wax at solids of 20, 15, 10, 5 and 0% after harvest. It is concluded that pears need cultivar specific wax coating application protocol and MAP bags to maximize the benefits and minimize the negative effects of wax coating and MAP (Yan Wang OSU OR). " Ripening behavior and quality of modified atmosphere packed Doyenne du Comice Pears during cold storage and transit was investigated. Control fruit packed in commercial perforated plastic liners started to show senescent core breakdown and lost the ripening capacity at 20 °C after 4 mo. of cold storage. LifeSapn® L257 MAP (15.8% O2 + 3.7% CO2) inhibited ethylene production, ascorbic acid degradation and malondialdehyde accumulation, and extended storage life up to 6 mo. with maintenance of fruit flesh firmness (FF) and skin color without commercially unacceptable level of physiological disorders (Yan Wang and David Sugar OSU OR). " Different conditions for water-jet cutting of various vegetables (celery, broccoli, romaine lettuce, iceberg lettuce, cabbage, among others) have been evaluated (Cantwell UCD CA). " Impact of temperature, modified atmospheres, 1-MCP and other treatments on marketable and nutritional quality of kale, as intact and salad-cut leaves, has been documented (Cantwell UCD CA). " The impact of damage and ripening conditions on the performance of tomatoes sliced at the red color stage is being determined. The general goal of this study is the identification of handling, varieties, and other parameters that would permit slicing at more advanced stage of color is being studied (Cantwell UCD CA with Buccheri Italy). " A new project that will identify the nutrient content of fresh-cut and frozen fruits and vegetables has started (Barrett UCD CA). " Field (preharvest) studies with fresh blueberry using food sanitizers or nutritional treatments to increase fruit quality at harvest were repeated a second year (Baldwin, Plotto, Narciso and Bai USDA/ARS USCSPL FL). " Study to determine optimum harvest time for new mandarin cultivars using sensory evaluation and correlating with volatile data was repeated a second year (Baldwin, Plotto, Narciso and Bai USDA/ARS USCSPL FL). " New clamshells that reduce water loss of small fruits without increasing decay, thereby extending shelf life were tested with strawberries and blueberries (Baldwin, Plotto, Narciso and Bai USDA/ARS USCSPL FL). " Optimum maturity of strawberries and blueberries and factors of eating quality were evaluated a second year as part of a funded SCIR grant (Brecht and Mitcham PIs) (Baldwin, Plotto, Narciso and Bai USDA/ARS USCSPL FL). " Investigation of optimal growth conditions for lactic acid bacteria (LAB) and bacteriocins production and determination of the correlations of pH, optical density (OD), bacteriocin activity and viability of LAB (Fan AAFC Kentville Canada). " Identification of bacteriocins produced by LAB strains isolated from various food sources (Fan and Song AAFC Kentville Canada). " Evaluation of microbial quality and shelf life of fresh-cut turnips and baby carrots (Fan AAFC Kentville Canada). " Evaluation of microbial quality and water quality of hydroponic leafy greens (Forney and Fan AAFC Kentville Canada). " Development of packaging and postharvest handling of novel hydroponic leafy green products (Forney AAFC Kentville Canada). " Enhancement of apple aroma through aroma volatile precursor treatments (Forney and Song AAFC Kentville Canada). " Determination of factors that impact the flavor chemistry (volatiles, sugars, acids) of blueberry and apple juice (Forney AAFC Kentville Canada). " Assessment of hydrogen peroxide as enhancer of shelf-life, nutrient quality and microbiologic quality of fresh-cut spinach (Bezanson and Hodges AAFC Kentville Canada). " A project to identify the optimal ripeness stage for processing mangoes into fresh-cut slices found that sensory quality during fresh-cut handling is best predicted by fruit firmness rather than color development or compositional measurements (Brecht, Huber, Sargent, Danyluk, Goodrich, Schneider, Simonne, Sims, Bartz UF FL). " Preliminary work established that pink stage tomatoes tolerate 4% but not 6% CO2 at 12.5C, and tolerate 6% CO2 at both 15 and 18C. Analysis of aroma volatiles in order to establish the chilling threshold in terms of aroma inhibition is underway (Brecht, Huber, Sargent, Danyluk, Goodrich, Schneider, Simonne, Sims, Bartz UF FL with ARS-Ft. Pierce). " It has been demonstrated the feasibility of hydrocooling strawberries, which allows the possibility of sanitization with resulting quality equal to or better than with forced-air cooling (Brecht, Huber, Sargent, Danyluk, Goodrich, Schneider, Simonne, Sims, Bartz UF FL). " Mature green tomatoes given a brief heated water treatment (2.5 min at 54C or 5 min at 52 C) prior to ethylene treatment and ripening were shown to develop or maintain better sensory quality, particularly when subsequently exposed to chilling temperatures of 5 or 12C, but tomatoes treated later in development (breaker/turning) did not respond as well (Brecht, Huber, Sargent, Danyluk, Goodrich, Schneider, Simonne, Sims, Bartz UF FL with ARS-Ft. Pierce). " Antimicrobial films made from wheat gluten containing naturally occurring cinnamaldehyde and natamycin have been developed and their effectiveness against main postharvest diseases has been demonstrated (Almenar MSU with Hernandez-Muñoz et al. IATA-CSIC Spain). " The effect of rigid containers on the shelf life of shell-on chestnuts at retail temperatures has been determined (Almenar MSU). " A multi-disciplinary, multi-institutional, multi-functional special emphasis project focused on enhancing the microbial safety and quality of ready-to-eat, fresh-cut produce via integrated research and outreach/training targeted at the processing, packaging, and retail distribution segments of the produce chain was presented to the S-294 members and (Almenar MSU). " (i) screening of available genotypes; (ii) reviewing the impact of most important pre-cutting handling practices; (iii) selecting feasible post-cutting anti-browning procedures; (iv) investigating possible benefits and/or possible damages on product quality, establishing both the optimal gas composition and the deleterious threshold concentrations which promote or accelerate degradation phenomena; (v) designing optimal modified-atmosphere packaging conditions are reported as steps of the research activity done to standardize the final quality of fresh-cut artichokes (Colelli Italy). " The effect of different agents, including calcium ascorbate, calcium chloride and ascorbic acid, on shelf-life of fresh-cut pears Packhams Triumph variety immediately after harvest and after 6 months of controlled atmosphere storage has been investigated (Pilizota Croatia). " The effect of L. cubeba oil, at different concentrations (50, 100 and 250 ppm) on shelf-life of fresh-cut pears Packhams Triumph variety has been investigated (Pilizota Croatia). Objective 3. Improve understanding of physiological mechanisms that affect fresh-cut product quality. " Collected first year data about the organic production systems on the yield, fruit quality and microbial safety of blackberries in order to improve yield, fruit quality, and food safety in fresh and processed markets (Zhao, Y., Strik, B. and Daeschel, M. OSU OR). " Internal browning disorder and fruit quality of Bartlett pears in modified atmosphere packaging during storage and transit have been studied. Bartlett pears harvested at commercial maturity were packed in LifeSpan® MAP, an experimental MAP and commercial perforated plastic bags (control) and stored in air at -1.1 °C. After 1 and 3 months of storage, samples of LifeSpan MAP and control fruit were transferred to rooms at temperatures of 2, 4.5, 7.5, and 10 °C for 3 weeks to simulate transit temperatures and the time required to reach distant markets.. LifeSpan MAP designed for pears appears to be suitable for Bartlett fruit stored at -1.1 °C for up to 4 months or storage for 3 months and a transportation duration of up to 3 weeks at 0-4.5 °C during the early season and at 0-2 °C during the late packing season. These conditions yielded fruit of high eating quality and without IB or over-ripening upon arrival at distant markets (Yan Wang and David Sugar OSU OR). " The transduction of a mechanically induced wound (e.g., cutting in preparation for fresh-cut) into a physiological response that produces increased phenolic metabolism, accumulation of phenolic compounds, and subsequent tissue browning has been studied (Saltveit UCD CA). " Physiological dysfunctions resulting from exposure to non-freezing, chilling temperatures greatly limits the storage life of chilling sensitive commodities (e.g., tomatoes, squash) appear to be less in fresh-cut commodities than in their previously whole progenitor (Saltveit UCD CA). " Gene expression in orange fruit infected by Huanglongbing. Predict orange fruit and juice quality by detecting population of Candidatus Liberibacter asiaticus in juices extracted from Huanglongbing infected fruit (Baldwin, Plotto, Narciso and Bai USDA/ARS USCSPL FL). " Influence of chilling and heating stresses on gene express and enzyme activities of LOX pathway and ascorbate-glutamine pathway (Baldwin, Plotto, Narciso and Bai USDA/ARS USCSPL FL). " Determination of heat treatments (boiling water and steam) on microbial survival and biochemical changes of fiddleheads stored in cold water at 0.5°C (Lihua Fan and John DeLong AAFC Kentville Canada). " Investigation of fruit maturity on the response of apples to heat stress (Lihua Fan, Charles Forney and Jun Song AAFC Kentville Canada). " Identification and characterization of volatile compounds responsible for fruit flavor using gas chromatography-mass spectroscopy-olfactory detection (GC-MS-OD) of apples and highbush blueberries (Charles Forney AAFC Kentville Canada). " Determination of the fatty acid composition of fiddleheads and the impact of postharvest treatments and storage (Charles Forney and John DeLong AAFC Kentville Canada). " A brief heated water treatment (2.5 min at 54C or 5 min at 52 C) was applied to mature green (prior to ethylene treatment) and breaker/turning tomatoes; the fruit were then ripened at 20C and/or stored at 5 or 12C; production of antioxidants, including carotenoids and phenolics, was stimulated, and antioxidant capacity (ORAS and FRAP) increased (Brecht, Huber, Sargent, Danyluk, Goodrich, Schneider, Simonne, Sims, Bartz UF FL with ARS-Ft. Pierce). " The effects of fresh-cut processing of postclimacteric papaya were evident in the marked loss of polyuronides, and xylose and galactose, suggesting a significant change in xyloglucans. Fresh-cut processing also caused a higher loss of total polyuronides and the neutral sugar galactose while increasing the recovery of chelator-soluble polyuronides (Brecht, Huber, Sargent, Danyluk, Goodrich, Schneider, Simonne, Sims, Bartz UF FL). " Fresh-cut apple tissue shows high sorption capacity for gaseous 1-MCP and 1-MCP sorption to fresh-cut apple is significantly reduced in tissue following short periods of tissue aging or application of the hydroxyl radical quencher hypotaurine (Brecht, Huber, Sargent, Danyluk, Goodrich, Schneider, Simonne, Sims, Bartz UF FL). " Post-controlled atmosphere air storage has been found to reduce browning in fresh-cut apple cultivars (Toivonen, Bach, and Delaquis AAFC Summerland Canada). Objective 4. Determine critical factors in controlled inoculation studies with human pathogens and surrogates that influence the outcome of quantitative microbial risk assessments. " Discovery of three rapid detection technologies (pre-analytical extraction, foam/vacuum extraction and hollow fiber detection, and chemical restriction fragment length polymorphism detection) for foodborne pathogens in many matrices including produce (Byron Brehm-Stecher ISU IA). " Symposium Originator and Co-Convenor: From Farm to Fork to Physician: Detection of Human Pathogens in the Environment, in Foods and in Clinical Specimens. International Association for Food Protection Annual Meeting, Milwaukee, WI (Shaw and Wilson ISU IA). " Assessment/modification of novel protocol for the recovery, enrichment and confirmation of carriage of verotoxigenic E. coli other than serotype 0157:H7 by intact field-grown Romaine lettuce and growth soils (Greg Bezanson and Pascal Delaquis AAFC Kentville Canada). " Work on pasteurized and unpasteurized almonds to address the effects of pasteurization treatments targeting Salmonella on quality parameters is being conducted (Barrett UCD CA). " Studies were undertaken to quantify the risks related to the consumer handling of fresh-cut produce. Temperature dependent growth models for Listeria monocytogenes, Salmonella spp., and Escherichia coli O157:H7 on fresh-cut celery and melons (cantaloupe, watermelon and honeydew) were constructed. The transfer of pathogens from hands and common kitchen surfaces to and from fresh cut celery, carrots, melon, and leafy greens, and during the simulated washing of cut leafy greens in the home was modeled (Brecht, Huber, Sargent, Danyluk, Goodrich, Schneider, Simonne, Sims, Bartz UF FL). " Risk factors during the production, harvest, and postharvest handling of fresh items were evaluated, including: transfer of Salmonella to tomatoes during harvesting, packing, and shipping; establishing tomato dump and flume tank operating standards for chlorine dioxide; and risk related to alternative harvesting and handling of small fruits (Brecht, Huber, Sargent, Danyluk, Goodrich, Schneider, Simonne, Sims, Bartz UF FL). " Methods of green fluorescent protein (GFP) gene labeling for Listeria, Salmonella and E. coli O157:H7 have been developed. They determined that E. coli O157:H7, Salmonella and Listeria strains can be effectively labeled with the GFP plasmid, which can be stable in some isolates for many generations without adversely affecting growth rates (Zhang, Doyle and Ma UGA GA). Objective 5. Evaluate and control unintentional and intentional microbial contamination of intact and fresh-cut produce. " The effects of immersing whole Serrano peppers in PRO-SAN (an EPA-approved vegetable wash) and its derivatives, on viability of Salmonella Saintpaul and quality characteristics of the peppers were investigated. PRO-SAN-based vegetable washes exhibited good potential for destroying S. Saintpaul on Serrano peppers without negatively altering quality attributes for up to 7 days of storage (Mendonca, Boylston and Wilson ISU IA). " The efficacy of various solutions of Pro-San" (Microcide, Inc®, Detroit, MI) was tested on romaine lettuce leaves against Salmonella enterica in a biofilm. Pro-San" LC and Pro-San" LC Soft were tested at two dilutions, 0.78% and 0.19%, at various time points and compared to a dry control and 150 ppm bleach solution, which is the currently used industry standard. Results indicated that the dry control, distilled water control, and 150 ppm bleach solution were not significantly different from each other, but were less effective than the Pro-San" solutions when the treatments were tested at room temperature. At refrigerated temperature, the same trend among treatments existed but only the 0.78% solutions were statistically more effective than the dry control and distilled water control, and only the 0.78% Pro-San" was more effective than the bleach solution (Mendonca, Boylston and Wilson ISU IA). " An integrated assessment of agronomic management practices, environment, and supply-chain risk factors on quality and safety of fresh spinach has been performed (Suslow UCD CA). " Plans were developed to assess the effectiveness of low temperature thermal treatments (LT3) on the microbiology of Listeria innocua on fresh-cut cantaloupe (Bezanson, Fan and Forney AAFC Kentville Canada). " Assessment of contaminated irrigation water and liquid dairy manure as reservoirs of enteric pathogens for field grown spinach and lettuce (Bezanson AAFC Kentville and Delaquis AAFC Summerland Canada). " Elucidation of dynamics of E. coli O157: H7 persistence on and migration from stored, fresh-cut spinach and lettuce (Bezanson AAFC Kentville Canada). " Determination of influence of environmental stress on the survival and infectivity of STEC on leafy vegetables (Bezanson and Bach AAFC Kentville Canada). " Experiments using small batches of Salmonella surface inoculated strawberries were conducted. At an inoculation level of 6.54 log cfu/strawberry, forced-air cooling resulted in a slight increase (6.66 logs units), while hydrocooling in 100 ppm sodium hypochlorite (7 min) resulted in 2.4 logs decrease in pathogen counts. A higher sanitizer concentration (200 ppm, 7 min) decreased Salmonella concentration to 3.07 log cfu/strawberry (Brecht, Huber, Sargent, Danyluk, Goodrich, Schneider, Simonne, Sims, Bartz UF FL). " Utilizing an overhead spray brush roller sanitation system, the efficacy of water, PAA, ClO2 and NaOCl on Salmonella inoculated tomatoes has been examined. After only 5 s, PAA reached a 2.8 log CFU/ml reduction, conversely, NaOCl, ClO2, and water each had a 1.9 log CFU/ml reduction. All sanitizers reached a 3-log unit reduction at 15 s, including water. PAA consistently achieved about a 1-log unit higher reduction than the other sanitizers for 5, 15, and 30 s treatment (Brecht, Huber, Sargent, Danyluk, Goodrich, Schneider, Simonne, Sims, Bartz UF FL). " An overview of on-farm intervention strategies for reducing pathogens during production of food products has been prepared (Doyle and Erickson UGA GA). " Field trials of compost heaps and determined that covered heaps maintained temperatures 7-15EC higher than those covered with fresh were conducted straw or left uncovered. These increased temperatures resulted in rapid E. coli reduction, thus validating recommendations by the EPA for covering fresh compost (Doyle, Erickson et al. UGA GA). " A chapter on the sources and methods of contamination of pathogen contamination in fresh and minimally processed produce was prepared. She detailed research on mechanisms employed by enteric pathogens to survive plant resistance as well as methods plants use to resist these invaders (Erickson UGA GA). " The Annual Review of Food Science Technology featured an overview of the internalization of foodborne pathogens by fresh produce (Erickson UGA GA). " The efficacy of slightly acidic electrolyzed water in reducing E. coli O157:H7 on whole lettuce leaves and tomatoes was investigated (Hung UGA GA). " The effectiveness of ozone, electrolyzed oxidizing water and UV light in killing E. coli O157:H7 on the calyx and skin of blueberries was studied (Hung and Kim UGA GA). " The treatment of tomatoes with levulinic acid and sodium dodecyl sulfate (SDS) to reduce E. coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes was examined (Doyle, Zhao and Zhao UGA GA). " The effects of inoculum level, temperature and relative humidity on the survival and colonization of E. coli O1157:H7 on spinach leaves were determined (Beuchat et al UGA GA). " Development and optimization a RT-qPCR method for the detection of viable but non-culturable (VBNC) Escherichia coli O157:H7 cells from lettuce (Toivonen, Bach, and Delaquis AAFC Summerland Canada). " Evaluation of the effects of endogenous and exogenous hydrogen peroxide on the persistence of pathogens on lettuce (Toivonen, Bach, and Delaquis AAFC Summerland Canada). " Evaluation of the effects of stress on the survival and infectivity of Verotoxin-producing Escherichia coli (VTEC) in ready-to-eat (RTE) foods (Toivonen, Bach, and Delaquis AAFC Summerland Canada).

Books: " Edible Coatings and Films to Improve Food Quality, Baldwin, E.A., Hagenmaier, R. and Bai, J. (eds.) Edible Coatings and Films to Improve Food Quality 2nd Edition, CRC Press, Taylor and Francis, Boca Raton. 448 pp. 2012 Book chapters: " Cheng, G. and Baldwin, E.A., Regulatory aspects of coatings, 383-415. Baldwin, E.A., Hagenmaier, R. and Bai, J. (eds.) Edible Coatings and Films to Improve Food Quality 2nd Edition, CRC Press, Taylor and Francis, Boca Raton. 448 pp. 2012 (Book Chapter) " Bai, J. and Plotto, A. Coatings for fresh fruits and vegetables, 185-242. Baldwin, E.A., Hagenmaier, R. and Bai, J. (eds.) Edible Coatings and Films to Improve Food Quality 2nd Edition, CRC Press, Taylor and Francis, Boca Raton. 448 pp. 2012 " Dea, S., Ghidelli, C., Perez-Gago, M.B. and Plotto, A. Coatings for minimally processed fruits and vegetables, 243-289. Baldwin, E.A., Hagenmaier, R. and Bai, J. (eds.) Edible Coatings and Films to Improve Food Quality 2nd Edition, CRC Press, Taylor and Francis, Boca Raton. 448 pp. 2012 " Forney, C.F. and E.S. Yaganza. 2011. Fresh-cut produce packaging and the use of biaxial stretched films, p. 143-164. In: M.T. DeMeuse (ed.) Biaxial Stretching of Film: Principles and Applications. Woodhead Publishing Ltd., Cambridge, U.K. " Forney, C.F. and W. Kalt. 2011. Blueberries and cranberries p. 51-73. In: L. Terry (ed.) Health Promoting Properties of Fruits and Vegetables. CABI, Wallingford, UK. " Baldwin, E.A. and Bai, J.: Physiology of fresh-cut Fruits and Vegetables, pp. 87-113. In O. Bartin-Belloso, O. and Soliva-Fortuny, R. (eds) Advances in Fresh-Cut Fruits and Vegetables Processing, CRC Press/Taylor Francis Group, Boca Raton. 410 pp. 2011. " Narciso, J., Parish, M. and Danyluk, M. 2012. Food safety of fruit, nut and berry products. in An Introduction to Food Safety. O.A. Oyarzrbal, ed. Springer, NY. 109-125. " Erickson, Marilyn C. 2012. Microbial Contamination chapter in Decontamination of Fresh and Minimally Processed Produce, VM Gomez-Lopez, ed. Wiley-Blackwell, Ames, IA, pp. 3-42. " Gonzalez-Aguilar, G. A., Kader, A.A., Brecht, J.K. and Toivonen, P.M.A. 2011. Fresh-cut tropical and subtropical fruit products, pages 381- 418. In: E. M. Yahia (ed), Postharvest biology and technology of tropical and subtropical fruits. Volume 1: Fundamental Issues. Woodhead Publishing Limited, Cambridge, UK. Peer-reviewed journals: " Mendonca, A., A. Daraba, D. Manu, and F. Woods. 2011. Inactivation of Salmonella enterica On Whole Tomatoes and Serrano Peppers Immersed In Pro-San, a Biodegradable Vegetable Sanitizer. Journal of Environmental Protection and Ecology, ISSN 1311-5065, Book II, pp.769 - 780 " Bezanson, G., Delaquis, Bach, S., McKellar, R., Topp, E., Gill, A., Blais, B., Gilmour, M. 2012. Comparative examination of E. coli O157:H7 survival on Romiane lettuce and in soil at two independent experimental sites. J. Food Protect. 75:480-487. " Cliff. M., J. Moran Trujillo, K. Stanich, P. Toivonen, and C. Forney. 2011. Determination and prediction of odor thresholds for odor active volatiles in a neutral apple juice matrix. J. Food Qual. 34(3):177-186. " DeLong, J.M., D.M. Hodges, R.K. Prange, C.F. Forney, P.M.A. Toivonen, M.C. Bishop, M. L. Elliot, and M.A. Jordan. 2011. The unique fatty acid and antioxidant composition of ostrich fern (Matteuccia struthiopteris) fiddleheads. Can. J. Plant Sci. 91:919-930. " Fan, L., D. Sharpe, E. Yang, C. Doucette, J. Song, L. Truelstrup Hansen, Y. Jiang and S. Fillmore. 2011. Antimicrobial effects of bacteriocins producing lactic acid bacteria isolated from various food sources. HortScience 46(9): S162-S163. " Fan, L., J. Song, C. Forney and M. Jordan. 2011. Fruit maturity affects the response of apples to heat stress. Postharvest Biology and Technology. 62: 35-42. " Forney, C.F. M.A. Jordan, L. Fan, C. Doucette, and R. Grant. 2011. Influence of packaging on loss of aroma and quality of diced red onions. Acta Hort. (in press). " Forney, C.F., R. Grant, L. Fan, C. Doucette, and M. A. Jordan. 2012 Market life of diced red onion subjected to modified atmosphere packaging and antimicrobial dips. Acta Hort (in press) " Forney, C.F., W. Kalt, M.A. Jordan, M.A. Vinqvist-Tymchuk, and S. Fillmore. 2011. Compositional changes in blueberry and cranberry fruit during ripening. Acta Hort. (in press). " Song, J., C.F. Forney, D. Moreau, M. Jordan, S. Fillmore, and K. Munro. 2011. Effect of fruit maturity and fresh cut on the respiration and volatile production of apple fruit. Acta Hort. (in press). " Yang, E., L. Fan, Y. Jiang, C. Doucette and S. Fillmore. 2011. Antimicrobial effect of natural preservatives a commercial fermentation product on fresh- cut onions. Journal of Food Protection. 74(3): 470-474. " Yang, E., L. Fan, Y. Jiang, C. Doucette and S. Fillmore. 2011. Antimicrobial activity of bacteriocin-producing lactic acid bacteria isolated from cheeses and yogurts. Applied Microbiology and Biotechnology Express (AMAB-D-11-01885, accepted). " Anthon, G.E. and D.M. Barrett. May 2012. Pectin methylesterase activity and other factors affecting pH and titratable acidity in processing tomatoes. Food Chemistry 132(2): 915-920. " Anthon, G.E., M. LeStrange and D.M. Barrett. May 2011. Changes in pH, acids, sugars and other quality parameters during extended vine holding of ripe processing tomatoes. Journal of the Science of Food and Agriculture 91(7):1175-1181. " Asavasanti, S., W. Ristenpart, P. Stroeve, and D.M. Barrett. Jan./Feb. 2011. Permeabilization of Plant Tissues by Monopolar Pulsed Electric Fields: Effect of Frequency. Journal of Food Science 76(1):E98-E111. " Barrett, D.M., and B. Lloyd. Jan. 2012. Advanced Preservation Methods and Nutrient Retention in Fruits and Vegetables. Journal of the Science of Food and Agriculture. 92(1): 7-22. " Cantwell, M. (ed. compiler). 2011. Fresh-cut Products: Maintaining Quality and Safety. Univ. California Postharvest Horticulture Series No. 10. UC Postharvest Research and Information Center (http://postharvest.ucdavis.edu/files/93535.pdf ). Hard copy and pen drive. " Gutiérrez-Rodríguez, E., H.J. Lieth, J.A. Jernstedt, J.M. Labavith, T.V. Suslow and M.I. Cantwell. 2011. Texture, composition and anatomy of spinach leaves in relation to nitrogen fertilization. J. Science Food Agriculture, in press. " Kumar, P., D.M. Barrett, M. Delwiche, and P. Stroeve. August 2011. Pulsed Electric Field Pretreatment of Switchgrass and Woodchips Species for Biofuels Production. Industrial & Engineering Chemistry Research 50(19): 10996-11001. " Bai, J., Baldwin, E.A. and Plotto, A. 2011. Stability of headspace volatiles in a 'Fallglo' tangerine juice matrix system at room temperature. Proc. Fla. State Hort. Soc. 124:207-212. " Bai, J., Plotto, A., Spott, R. 2011. Ethanol vapor and saprophytic yeast treatments reduce decay and maintain quality of intact and fresh-cut sweet cherries. Postharvest Biol. Technol. 62:204-212. " Bai, J., R. Graves, and Y. Wang. 2011. Development of chromatography system for simultaneous measurement of gas components in storage atmosphere of horticultural crops. Proc. Fla. State Hort. Soc. 124: 235-240. " Baldwin, E.A., Bai, J., Plotto, A., Cameron, R., Luzio, G., Narciso, J., Manthey, J.A., Widmer, W.W. and Ford, B.L. 2012. Effect of extraction method on quality of orange juice: hand-squeezed, commercial-fresh squeezed and processed. J. Sci. Food Agric. DOI 10.1002/jsfa.5587 " Chambers, A., Whitaker, V., Gibbs, B., Plotto, A. and Folta, K. 2012. Detection of the linalool-producing NES1 variant across diverse strawberry (Fragaria spp.) accessions. Plant Breeding. doi:10.1111/j.1439-0523.2012.01959.x " Du, X., Plotto, A., Baldwin, E., and Rouseff, R. 2011. Evaluation of volatiles from two subtropical strawberry cultivars using GCOlfactometry, GC-MS, odor activity values, and sensory analysis. J. Agric. Food Chem. 59:12569-12577. " Du, X., Plotto, A., Song, M., Olmstead, J., Rouseff, R. 2011. Volatile composition of four southern highbush blueberry cultivars and effect of growing location and harvest date. J. Agric. Food Chem. 59:8347-8357. " House, L.A., Gao, Z., Spreen, T.H., Gmitter, F.G., Jr., Valim, M.F., Plotto, A., Baldwin, E. A. 2011. Consumer preference for mandarins: Implications of a sensory analysis. Agribusiness: An International Journal. 27:450-464. " Miyazaki, T., Plotto, A., Baldwin, E.A., Reyes-De-Corcuera, J.I., Gmitter, F.G., Jr. 2012. Aroma characterization of tangerine hybrids by gas-chromatography-olfactometry and sensory evaluation. J. Sci. Food Agric. 92:727-735. " Plotto, A., Baldwin, E., McCollum, G.T. and Gmitter, G. 2011. Sensory evaluation of tangerine hybrids at multiple harvests. Proc. Fla. State Hort. Soc. 124:260-263. " Sitther, V., Harris, D.L., Dhekney, S.A., Bai, J., Baldwin, E.A. and Yakav, A.K. 2011. Total phenol content of guava fruit and development of an in Vitro regenertion protocol amenable to genetic improvement. Int., J. Food Safery, Nutrition and Public Health. 4(3/4):225-236. " Whitaker, V.M., Chandler, C.K., Santos, B.M., Peres. N., do Nascimento Nunes, M.C., Plotto, A. and Sims, C.A. Winterstar" (FL 05-107) strawberry. 2012. HortScience. 47:296-298. " Zhang, D., Yu, B., Bai, J., Shu, Q., Su, J., Zheng, X., Kong, D., Teng, Y. 2012. Effects of high temperatures on UV-B/visible irradiation induced postharvest anthocyanin accumulation in `Yunhongli No. 1- (Pyrus pyrifolia Nakai) pears. Scientia Horticulturae. 134:53-59. " Bisha,B., A. Perez-Mendez, M.D. Danyluk, and L.D. Goodridge. 2011. Evaluation of modified Moore swabs and continuous flow centrifugation for concentration of Salmonella spp. and Escherichia coli O157:H7 from large volumes of water. J. Food Prot. 74: 1934-1937. " Cevallos-Cevallos, J.M., M.D. Danyluk, J. I. Reyes-De-Corcuera. 2011. GC-MS based metabolomics for rapid detection of Escherichia coli O157:H7 and Salmonella Typhimurium, Salmonella Muenchen and Salmonlla Hartford in beef and chicken. J. Food Sci. 76: M238-M246. " Chang, A.S. and K.R. Schneider. 2012. Evaluation of overhead spray-applied sanitizers for the reduction of Salmonella on tomato surfaces. J. Food Sci. 77: M65-M69. " Danyluk, M.D. and D.W. Schaffner. 2011. Quantitative microbial assessment for E. coli O157:H7 in leafy greens from farm to consumption using published data. J. Food Prot. 74: 700-708. " Danyluk, M.D., L.M. Friedrich, C. Bousquet, R.M. Goodrich-Schneider, M.E. Parish, and R. Rouseff. 2011. Prevalence, amounts, spoilage, and mitigation of Alicyclobacillus spp. in tropical and subtropical fruit concentrates. Food Microbiol. 28: 472-477. " Dea, S., J.K. Brecht, M.C.N. Nunes, and E.A. Baldwin. 2011. Optimal ripeness stage for processing mangoes into fresh-cut slices. HortTechnology (in press) " Deltsidis, A.I., E.D. Pliakoni, and J.K. Brecht. 2011. Establishing CO2 tolerance of pink tomatoes in MAP at elevated handling temperatures. Proc. Fla. State. Hort. Soc. 124: 241-245. " Ergun, M., Y. Karakurt, and D.J. Huber. 2011. Cell wall modification in 1-methylcyclopropene-treated post-climacteric fresh-cut and intact papaya fruit. Plant Growth Regul. 65: 485-494. " Fatica, M.K. and K.R. Schneider. 2011. Salmonella and produce: Survival in the plant environment and implications in food safety. Virulence 2: 573-579. " Jacomino, A.P., S.A. Sargent, A.D. Berry, and J.K. Brecht. 2011. Potential for grading, sanitizing and hydrocooling fresh strawberries. Proc. Fla. State. Hort. Soc. 124: 221-226. " Lee, J.S., D.J. Huber, C.B. Watkins, and B. Hurr. 2012. Influence of wounding and aging on 1-MCP sorption and metabolism in fresh-cut tissue and cell-free homogenates from apple fruit. Postharvest Biol. Technol. 67: 52-58. " Loayza, F.E., A. Plotto, E.A. Baldwin, and J.K. Brecht. 2010. Evaluation of the impact of hot water treatment on the sensory quality of fresh tomatoes. Acta Hort. (in press) " Perez-Cacho, P.R., M.D. Danyluk, and R. Rouseff. 2011. GC-MS quantification and sensory thresholds of guaiacol in orange juice and the relationship between guaiacol and Alicyclobacillus. Food Chem. 129: 45-50 " Strawn, L.K., K.R. Schneider, and M.D. Danyluk. 2011. Microbial safety of tropical fruits. Crit. Rev. Food Sci. Nutr. 51: 132-145. " Tsouvaltzis, P., A.I. Deltsidis, and J.K. Brecht. 2011. A delay between hot water treatment and processing of potato tubers reduces browning development on fresh-cut slices. HortScience 46: 1282-1286. " Choi, S, J Bang, H Kim, LR Beuchat, and JH Ryu. 2011. Survival and colonization of Escherichia coli O157:H7 on spinach leaves as affected by inoculum level and carrier, temperature and relative humidity. Journal of Applied Microbiology, 111: 14651472. doi: 10.1111/j.1365-2672.2011.05175.x. " Doyle, Michael P., and Marilyn C. Erickson. 2012. Opportunities for mitigating pathogen contamination during on-farm food production. International Journal of Food Microbiology, Vol. 152, Issue 3, 16 January 2012, Pages 54-74, ISSN 0168-1605, 10.1016/j.ijfoodmicro.2011.02.037 " Shepherd, Marion W., Jr., Jinkyung Kim, Zipping Jiang, Michael P. Doyle, and Marilyn C. Erickson. 2011. Evaluation of Physical Coverings Used to Control Escherichia coli O157:H7 at the Compost Heap Surface. Applied and Environmental Microbiology. July 2011, p 5044-5049, ISSN 0099-2240/11/$12.00 doi: 10.1128/AEM.02940-10. " Erickson, Marilyn C. 2012. Internalization of Fresh Produce by Food borne Pathogens. Annual Review of Food Science and Technology, Vol 3, 283-310, April 2012. " Kim, C and Hung, YC. 2012. Inactivation of E. coli O157:H7 on Blueberries by Electrolyzed Water, Ultraviolet Light, and Ozone. Journal of Food Science, 77: M206M211. doi:10.1111/j.1750-3841.2011.02595.x " Ma L, G Zhang and MP Doyle. 2011. Green Fluorescent Protein Labeling of Listeria, Salmonella, and Escherichia coli O157:H7 for Safety-Related Studies. PLoS ONE 6(4): e18083. doi:10.1371/journal.pone.0018083 " Pangloli, P and Hung, YC. 2011. Efficacy of Slightly Acidic Electrolyzed Water in Killing or Reducing Escherichia coli O157:H7 on Iceberg Lettuce and Tomatoes under Simulated Food Service Operation Conditions. Journal of Food Science, 76: M361M366. doi: 10.1111/j.1750-3841.2011.02219.x " Joo, M.; Merkel, C.; Auras, R.; Almenar, E. 2012. Development and characterization of antimicrobial poly(lactic acid) containing trans-2-hexenal trapped into cyclodextrins. International Journal of Food Microbiology, 153: 297-305 (http://dx.doi.org/10.1016/j.ijfoodmicro.2011.11.015). " Joo, M.; Auras, R.; Almenar, E. 2011. Preparation and characterization of blends made of poly(lactic acid) and ²-cyclodrextrins: improvement of the blend properties by suing a masterbatch. Carbohydrate Polymers, 86(2): 1022-1030 (http://dx.doi.org/10.1016/j.carbpol.2011.05.058). " Almenar, E. 2011. Cherries in microperforated bio-based containers. Good Fruit Grower. April, 1st, 2011 (http://www.goodfruit.com/Good-Fruit-Grower/Web-2011/Sustainable-cherry-packaging/) " Bach, S.J., Lu, C., Delaquis, P. and Toivonen, P.M.A. 2011. Cutting Romaine lettuce while submerged in sanitizer improves efficacy of sodium hypochlorite. The Grower, 61(5): 20. " Dinu, L.-D. and Bach, S. 2011. Induction of viable but non culturable Escherichia coli O157:H7 in the phyllosphere of lettuce: a food safety risk factor. Appl. Environ. Microbiol. 77:8295-8302 " Toivonen, P.M.A. and Hodges, D.M. 2011. Abiotic stress in harvested fruits and vegetables, pages 39-58. In: Abiotic Stress in Plants - Mechanisms and Adaptations. Intech Open Access Publishers, Rijeka, Croatia. " Toivonen, P.M.A., Lu, C., Delaquis, P. and Bach, S. 2011. Slicing under chlorinated water improves the disinfection of fresh-cut Romaine lettuce. J. Food Protect. 74: 2142-2147. " Tardelli, F., Guidi, L., Massai, R., and Toivonen, P.M.A. 2011. Effects of 1-methylcyclopropene and post-controlled atmosphere air storage treatments on fresh-cut Ambrosia" apple slices. J. Sci. Food Agr. (accepted). " Bezanson, G, Delaquis, P, Bach, S, McKellar, R, Topp, E, Gill, A, Blais, B and Gilmour, M. 2011. A comparative examination of Escherichia coli O157:H7 survival on Romaine lettuce and in soil at two independent experimental sites. J. Food Protect. 75:480-487. " McKellar, R and Delaquis, P. 2011. Development of a dynamic growth-death model for Escherichia coli O157:H7 in minimally processed leafy green vegetables. Internat. J. Food Microbiol. 51:7-14. " McKellar, RC, LeBlanc, DI, Lu, J and Delaquis, P. 2012. Simulation of Escherichia coli O157:H7 behaviour in fresh-cut lettuce under dynamic temperature conditions during distribution from processing to retail. Foodborne Path. Dis. 9:239-244. " Hashemi Beni, L, Villeneuve, S, LeBlanc, DI, Côté, K, Fazil, A, Otten, A, McKellar, R and Delaquis, P. 2012. Spatio-temporal assessment of food safety risks in Canadian food distribution systems using GIS. Spatial and Spatio-temporal Epidemiology. http://dx.doi.org/10.1016/j.sste.2012.02.009. " Piercey, M, Budge, SM, Mazzanti, G, Delaquis, P, Paulson, AT and and Truelstrup Hansen, L. 2012. Antimicrobial activity of cyclodextrin entrapped allyl isothiocyanate in a model system and packaged fresh-cut onions. Food Microbiol. 30: 213-218 Oral presentations, posters and abstracts: " Brehm-Stecher, Byron. 2011. Rapid Detection of Pathogens in Complex Food Matrices. IFT Session Abstract, New Orleans, LA and Sample Prep 2011, San Diego, CA and Society for Laboratory Automatic, Palm Spring CA " Kim, H.J. and B. Brehm-Stecher. June 16th-17th, 2011. Foam/Vacuum Extraction and Hollow Fiber Concentration for Detection of Foodborne Pathogens. NSF Conference on Novel Sampling and Sensors for Improving Food Safety, Georgia Tech, Atlanta, GA " Brehm-Stcher, Byron. June 30th-July 1st, 2011. Chemical Restriction Length Fragment Polymorphism (CRLFP) for Detection and Characterization of Bacterial Pathogens. Advances in Biotedetecion & Biosensors, European Lab Automation Meeting, Hamburg, Germany Abstract " Brehm-Stecher, B., M. Tortorello, and L. Jaykus. 2011. Symposium Originator and Co-Convenor: From Farm to Fork to Physician: Detection of Human Pathogens in the Environment, in Foods and in Clinical Specimens. International Association for Food Protection Annual Meeting, Milwaukee, WI " Fan, L. 2011. Antimicrobial activities and applications of bacteriocins producing lactic acid bacteria. The 9th International Conference of Food Science and Technology. Invited speaker. (Abstract). " Almenar, E.; Humes, A.; Lee, R.; Sobon, S.; Johnson, D.; Morse, R.; Sidiqq, M.; Haret, J; .Ryser, E.; Harte, B. 2011. A new marketing approach for minimally processed onions. UNITED FRESH 2011. May, 3, 2011. New Orleans, LA, USA. " Fan, L., E. Yang, C. Doucette, J. Song, Y. Jiang, S. Fillmore and B. Walker. 2011. Antimicrobial effect of commercial fermentation product and edible coating on fresh-cut squashes. HortScience 46(9): S341. (Abstract) " Fan, L., E. Yang, C. Doucette, Y. Jiang and S. Fillmore. 2011. Antimicrobial effect of nature seal and commercial fermented product on fresh-cut squashes. Poster presentation. United Fresh-2011 (Abstract). " Forney, C.F., L. Fan, J. Song and M.A. Jordan. 2011. Heat stress and fruit maturity affects the volatile composition of apples during storage. HortScience 46(9): S271. (Abstract) " Bayogan, E. and M. Cantwell. 2011. Postharvest Quality and Chilling Tolerance of Bell Pepper Varieties. Amer. Soc. Hort. Sci. Annual Conf., Kona, Hawaii, 25-28 Sept 2011. Abstract #235 and poster. HortScience 46(9): S332. " Cantwell, M. and G. Hong. 2011. Composition and postharvest performance of broccoli varieties. Amer. Soc. Hort. Sci. Annual Conf., Kona, Hawaii, 25-28 Sept 2011. Abstract #236 and poster. HortScience 46(9): S332. " Tiwari G., Slaughter D.C., Cantwell M. 2011. Prediction of maturity in green tomatoes using visible and near infrared spectroscopy. IFT Annual Meeting & Food Expo, June 11 - 14, 2011, New Orleans, Louisiana, USA. Abstract and oral presentation. " Loayza, F.E., Plotto, A., Baldwin, E., Bai, J., Dea, S., and Brecht, J.K., 2010. Evaluation of the impact of hot water treatment on the sensory quality of fresh tomatoes in cold storage. ASHS Annu. Mtg., Palm Desert, CA. Abstract. " Lyons, Shawn M, Mark A Harrison, and S Edward Law. 2011. Electrostatic application of antimicrobial sprays to sanitize food handling and processing surfaces for enhanced food safety. Journal of Physics, Conference Series Vol. 301, No. 1. IOPScience. " Zhao, Tong, Ping Zhao and Michael P. Doyle. 2011. Inactivation of Foodborne Pathogens on Tomatoes by Levulinic Acid Plus Sodium Dodecyl Sulfate. Poster presented at 12th ASEAN Food Conference, 16-18 June 2011, BITEC Bagna, Bangkok, Thailand. " Nuevas tendencias en empaques para productos perecederos. II International Conference on Postharvest Quality Managment of Horticultural Produtcs of Interst for Tropic Regions (II Conferencia Internacional en Manejo Poscosecha y Calidad de productos Hortícolas de Interés en el Trópico). November 3, 2011. Bogotá, Colombia (Guest speaker). " Balaguer, M.P.; Almenar, E.; Gavara, R.; Hernández-Muñoz, P. 2011. Antifungal activity of biopolymers made from wheat gluten containing naturally occurring cinnamaldehyde and natamycin. HortScience 46 (9): S 335. " Rai, D.R.; Almenar, E.; Min J.J. 2012. Estimation of controlled release of Trans 2E Hexanal under varying relative humidity conditions for food packaging application. 21st Indian Convention of Food Scientists and Technologists. January, 20-21, 2012. Pune, Maharashtra, India. " Toivonen, P.M.A., Lu, C., Delaquis, P. and Bach, S. 2011. Timing of sanitizer application reduces microbial counts on fresh-cut Romaine lettuce. 2nd International Conference on Quality Management of Fresh-Cut Produce from July 17-21, 2011, Torino, Italy.