Brief Summary of Minutes of Annual Meeting

Skaneateles, NY
Steve Albrecht, 2002 Chair, welcomed all participants to the meeting and brought the meeting to order.
Janice Thies, local organizer, presented the agenda and explained the local arrangements.
Administrative Report - Gerald (Skip) Jubb
Skip Jubb presented the Administrative Report. Unfortunately, Kriton was unable to join us due to a recent illness. As funding is getting tighter all the time, Skip advised us to emphasize high project quality and tangible outcomes. We are to work only on our defined objectives and to stay focused. Our annual report must be posted within 60 days of this meeting on the new National Information Management Support System (NIMSS). Format of final report is responsibility of the Chair (Steve Albrecht). David Sylvia manages the web page and Mike Mullen hosts the listserv for email.
Questions/Discussion:
It was apparent to all participants that funding for travel to the Annual Meeting is not managed equitably between institutions. The formula, however, is set by the states; therefore there is no way to ‘normalize‘ it.
The group acknowledged with sadness the passing of Bob Klucas, who joined the group in the early 1980s and contributed to it for over 20 years.
The remainder of the day was given over to group reports from all three objectives.
Peter Hartel - Obj #1
Peter provided an update of the work in Georgia on bacterial source tracking. Key points were:
1. The Hartel group has a laser focus on Obj #1 and has been very successful in obtaining extramural funding.
2. GA/ID collaborative E. coli work will appear in the Jour. of Environmental Quality.
3. Microbial source tracking -focus has been on E. coli, however, enormous total diversity and temporal variability is forcing them to look for another indicator - E. faecalis is looking promising as it is essentially only found in humans, wild birds and poultry.
Dominique Godfrey - Obj #1
Dominique presented a detailed report of this year‘s outcomes by the Georgia group. The primary data are contained in their Annual Report. Two key points were:
1. The greatest weakness for microbial source tracking is SAMPLING. The Georgia group is now undertaking intense targeted sampling. E. faecalis is the focus organism.
2. The fly in the ointment is that the fundamental premise for the use of indicator organisms is that they do not persist in the environment. This is likely not true for E. coli.
Questions and discussion for ALL reports are given in detail in the Meeting Minutes located on the S-297 website.
BREAK - Discussion on European Collaboration
The group was in favor of increasing collaboration overseas and developing new links. It was suggested that the Annual Meeting might be held in Europe in 2 years to facilitate integration and collaboration with European colleagues.
Jim Entry - Obj #1
Jim reported on his collaborative work with Peter Hartel on tracking E. coli in the Rock Creek catchment in ID. Limited data are available, however, contamination appears of human origin, not cattle. They will now apply intense targeted sampling. Jim has 3 years funding to do this. He will be sending the ID samples to the riboprinter in GA.
Chuck Hagedorn- Obj #1
Chuck reported on their work in the Chesapeake Bay. Antibiotic resistance, Biolog and PFGE are being used as tracking tools. They have found that some species of Enterococcus are related to certain hosts and not others and that the use of phenotypic markers only yields broad categories. They have been doing monthly sampling of E. coli and Enterococcus for one year, and have picked up several storm events. A human signature is seen during the wet season. Their aim is to contribute to TMDLs. To date, only 1/3 of state‘s waters have been evaluated. Chuck presented a poster at ASM and has a paper coming out in JEQ shortly on this work.
Scott Angle - Obj #2
Scott spent 5 mos. at Univ. of Melbourne with Alan Baker. He taught a joint class (MD and Aus) on phytoremediation, which was recorded on a CD. Scott is studying rhizosphere ecology of hyperaccumulators and looking for potential inoculant organisms. Different plants are being examined. He has isolated 250 bacteria - mostly pseudomonads, and has found 4 that increased uptake of Ni considerably. One that increases uptake by over 30%. Scott also reported on his work in constructed Rain Guard systems in which plant heavy metal hyperaccumulators are planted to take up Zn from tires. Anaerobic denitrification, C decomposition and decreased erosion are other benefits of the Rain Guards.
Cindy Nakatsu - Obj #2
At Pudue, remediation work is focusing on site assessment. Real-time PCR is being used to target aerobic oxygenase genes. A remediation method is put in place and then evaluated for increasing degradation. They are following gene copy number to see if it increases over time. They are also monitoring wells. This work is soon to be published.
David Zuberer - Obj #2
David reported on their work on water-soluble C in turf grass (urban Agriculture), where they are particularly interested in C dynamics and bioclogging in sand-based root zones. Although the greens‘ design is aeration and drainage friendly, they find a black zone developing after a few years. ‘Snake oil‘ products, that are being sold to greens-keepers as cure-alls, were discussed extensively.
Janice Thies - Obj #1 and #3
Cornell University hosted a workshop to train S-297 participants in the denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphism (TRFLP) analyses for soil microbial community analysis 6/8-11/02.
Cornell report - Federal Formula Funds funding has been obtained to begin collaborative work in Obj #1 with Hartel/Hagedorn/Emerson et al., beginning 10/1/02.
Bill Hickey - Obj #2
Bill reported on his work in characterizing PAH degrading bacteria isolated from humic acid (HA) solutions. He is studying the bioavailability of phenanthrene, which is sorbed by OM and has a low aqueous solubility. The hypothesis being tested is that enrichment systems with sorbed PAH may yield organisms relevant to biodegradation in soil. REP PCR fingerprints and 16S sequence analysis are being used to characterize PAH degraders. Mechanisms of degradation are also being studied.
David Sylvia - Obj #3
Dave reported on studies in dune stabilization. They have examined compatible plant/mycorrhizal fungus ecotype combinations from micro-propagated sea oats planted on FL beaches. Fungal diversity is being characterized by large subunit rRNA gene amplification and sequencing at four geographically diverse sites. They have found:
1. as much intra-site variability as inter-site variability.
2. a range of symbiotic effectiveness within the beach dune system, which affords the opportunity for selection of superior AM fungal-host combinations.
3. the origin of host and AM fungi has little predictive value in screening these combinations.
Shiping Deng - Obj #2
Shiping is studying nitroaromatic contamination and associated degrading bacteria in TNT contaminated soils. She has been conducting bioreactor studies with engineers. TNT-RDX-HMX - remediation sometimes works, sometimes not. The impact of contamination on soil N pools and the microbial community are being investigated. Microbial biomass, enzyme activity and total recoverable bacteria are all seriously affected in contaminated soils. Controlled studies are also in progress in which TNT has been added to healthy soils and the community response measured. DGGE and 16S sequencing of isolates are being conducted.
Textbook Committee - David Sylvia
Principles and Application of Soil Microbiology - 2nd Edition. David reviewed for us the proposed chapters and authors. These can be found on the S-297 website.
Steve Albrecht -
Steve is studying organic matter (OM) dynamics in varying tillage systems in Eastern OR, where low soil OM is the primary problem. They have found that:
1. Tillage that returns residue and low-till practices increase soil OM.
2. Light fraction (sodium iodide or sodium tungstate) centrifugation is correlated with reduced tillage operations.
Steve has also been working on:
1. building a model for C sequestration,
2. examining microbial diversity in different tillage operations, and
3. N mineralization studies post manure application in sandy soils in areas new to cattle production.
Ping Wang-
Ping reported on tillage trials in OH (long term no-till for 45 y). TRFLP is being used to compare community structure between tillage treatments. They are also monitoring animal pathogens by examining leachate for E. coli O157:H7 and Cryptosporidium. NOTE: Warren Dick - message delivered by Ping Wang. Warren would be glad to develop a proposal or cooperate with like-minded researchers. His projects can be linked into all 3 objectives.
Chuck Hagedorn-
Announced the newsletter devoted to bacterial source tracking "Environmental Detection News." Copies of the first issue were distributed. The newsletter is meant to be a more informal information-sharing medium.

Textbook discussion
David Sylvia reported that they expect to have the new edition on the shelf for Fall 2003. It should be to Prentice Hall by end of this year. The Laboratory manual is also in the works. Prentice Hall will not be the publisher. Dave requested sharing of exercises in our courses that might be useful, including molecular exercises. The 2nd edition of the textbook will be dedicated to the memory of Bob Klucas.
Election of officers and selection of next year‘s meeting site
Janice Thies is the chair for 2002-03. The chair-elect position was open for election. There were two self-nominations: Jim Entry and Mary Savin. These nominations were both seconded by Cindy Nakatsu. The vote was taken by a show of hands. Mary Savin was elected by a vote of 10 to 0.
Next year‘s meeting site:
Next year‘s meeting will be held in Puerto Rico, June 2-4, 2003. Eduardo Schroeder will be the local host in concert with Peter Hartel. Other members of the planning committee are: Jim Entry and Robin Kuntz.
Discussion on future collaborative grants - whole group
The following ideas and funding sources were discussed:
Bacterial source tracking:
1. National Park Service, Yellowstone, is interested.
2. Coastal Zone management, the Sea Grant Program , 319 Funds and EPA.
Biosafety issues:
1. The Military (DOD) - Camps need baselines established.
2. The STAR program
3. Local/State Water Quality initiatives.
Other funding sources: NSF - Microbial Observatories Program.
We all wish Kriton the very best. On behalf of group we wish him a speedy recovery.
Where does S-297 stand in the Multistate program?
We would appreciate if the station directors were to provide feedback of a substantive nature. We would also deeply appreciate some encouragement and support for the work we are doing - if they think our research is worthy of it. Administratively, how do we remove participants that are no longer active? Peter Hartel proposed that if no progress report has been received in the last 2 years, which is current project‘s duration, then remove the participant from the list. In order to stay on, they must submit a progress report within the next 30 days. Steve Albrecht will communicate this in conjunction to soliciting input to the annual report. He will also contact Skip Jubb to clarify the membership list of record and email the current list to all participants.
The chair, Steve Albrecht, thanked our host, Janice Thies, for making the arrangements for this year‘s meeting.
Move to adjourn: J. Thies
Second: D. Zuberer
Meeting was adjourned at 12:01 pm

A short-course on ‘Molecular Techniques for the Analysis of Microbial Community Structure in Soil and the Rhizosphere‘ (hosted by J. Theis in collaboration with Cindy Nakatsu [Purdue University] and Terry Marsh [Michigan State University] at Cornell Univ., 6/8-11/02) was taught for the S-297 principal investigators, their students and technicians.

Objective 1: In order to improve water quality where high fecal coliform numbers have been detected, Escherichia coli has been commonly used as a target organism to determine the potential sources. In addition to investigations of E. coli, we are also studying other bacteria (e.g. Enterococcus faecalis) found in sewage and manure. Ribotyping the microorganisms is used by many laboratories participating in this Project to differentiate between microorganisms found in human sewage and microorganisms found in either wild or domestic animals. Research in Georgia found that human sewage samples contained Ent. faecalis, but high percentage of broiler litters contained no Ent. faecalis (some contained low numbers). Percentages of Ent. faecalis were high in screech owl and wild turkey, but were low or zero in great horned owl, pelican, robin, and sea gull. No enterococci were observed in Canada goose, dove, and pigeon. The initial isolation media had no effect on isolating Ent. faecalis. Chickens had Ent. faecalis, but the litter strongly selected against the organism, suggesting that broiler litter is not an important source of Ent. faecalis to the environment. The presence of Ent. faecalis in individual species of wild birds was variable. Two watersheds in Western Puerto Rico are being examined for fecal coliforms. Initial sampling of a portion of the Yaguez River (PR) indicated high numbers of enterobacteria. Work is continuing to determine the source of E. coli contamination in Rock Creek area and several other streams in southern Idaho. Two hundred and forty ribotypes were identified from 451 E. coli isolates. fecal samples of two herds of Black Angus cattle in Georgia. Isolates from Idaho and Georgia will establish temporal variability, whereas isolates from Georgia and Puerto Rico will establish geographic variability. An E. coli ribotype database is being developed for the state of Delaware using isolates recovered from various hosts (wildlife, domesticated animals, and humans)

A large project on the Mill Creek Watershed (Montgomery County, VA) to determine source-tracking classifications (human vs. livestock vs. wildlife) as a function of sampling frequency was completed. The project was performed on both E. coli and Enterococcus, with antibiotic resistance analysis, carbon source utilization, and pulsed-field gel electrophoresis as source-tracking tools. Cooperative work (J. Harwood) will compare results to those obtained with ribotyping. The high ARCC of the large library demonstrates that isolates from different regions can be put together and it is not necessary to build a new large library with every new watershed. There is the distinct possibility that livestock so overwhelmed all other sources, when the cattle inventory in Mill Creek (approx. 3,800 animals) was considered, that the lack of obvious seasonal variation regarding livestock was simply a function of the predominant land use pattern devoted to pasture and cattle in the Mill Creek watershed. Two east Tennessee watersheds have generally characterized with respect to total coliforms, E. coli, fecal coliforms and enterococcus. Upcoming plans are to look at impacts of Best Management Practices on stream bacteriological quality.

In addition to ribotyping, other genetic fingerprinting methods to differentiate microorganisms, including E. coli, in the environment are being compared. Methods being tested are: rep-PCR (repetitive sequences, using both REP and BOXA1R primers, using the Polymerase Chain Reaction), AFLP (Amplified Fragment Length Polymorphism), PFGE (Pulsed-Field Gel Electrophoresis), and ribotyping. The accuracy of these various methods is now being tested more extensively on animal fecal sources. E. coli populations of animals from different facilities in Indiana are being examined to determine genetic variation between isolates from known hosts collected at different facilities in a limited geographic region. And differences in genetic fingerprint patterns of isolates collected from pig manure applied to agricultural fields and those collected from underlying tile drains after transport through the soil profile will be examined.

Objective 2: Research in Maryland showed that several microbial isolates from the rhizosphere of Alyssum murale were capable of increasing Ni uptake into the plant. Field trails using these associations will be conducted next year. The impact of 2,4,6-trinitrotoluene (TNT), hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), and octahydrol-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) contaminations on the chemical and microbiological properties of soils in Oklahoma was evaluated.

A technique based on real time PCR amplification of aromatic oxygenase genes was developed to detect and quantify a number of aromatic catabolic genes in environmental samples. Groundwater samples from gasoline-contaminated sites in Indiana were studied. In field samples, aromatic oxygenase genes were detected in groundwater monitoring wells with current or recent petroleum contamination but not in wells without a history of contamination, confirming that this technology is appropriate for monitoring pollutant biodegradation. In Alabama strains of Pseudomonas putida and Rhodococcus erythropolis were found to degrade soil-sorbed biphenyl. Results suggest that cell attachment ability was important for the access of soil-sorbed biphenyl and that chemotaxic ability was significant in scavenging sorbed biphenyl.

The effect of fertilizer addition and vegetation establishment on remediation of crude oil-contaminated soil was studied in Arkansas. Fertilizer addition and vegetation establishment significantly increased total bacterial and polycyclic aromatic hydrocarbon (PAH) degrader numbers. The fertilized-vegetated plots had significantly greater reductions in TPH levels as compared to the control. The plots are established and will continue to be monitored over the next several years to determine the effects of nutrient additions and vegetation on remediation and microbial ecology. Greenhouse studies are continuing to provide useful information related to selection of plant species suitable for establishment at crude oil-contaminated sites. The effect of compost layers on the retention and transport of atrazine and P were determined in several Homestead, FL area soils. A column study demonstrated that atrazine and P leached out slower in soil with compost than in soil without compost.

Objective 3: The effect of crop rotation on rhizobacterial diversity in a Norfolk soil (South Carolina) was studied for three years. Root bacteria were identified by GC-FAME analysis. Biochemical assay and PCR technology were used to evaluate the denitrification potential for 200 rhizobacteria. Results from research in Indiana have demonstrated that microbial community fingerprinting techniques (16S rDNA PCR and DGGE) can be used to show differences in the microbial community structure influenced by the environment. The rhizosphere microbial community of corn plants was investigated in order to determine if the same populations are affected by different agronomic treatments over different years. Cluster analysis, using principal components analysis (PCA), revealed that in all three years the microbial community profiles grouped according to agronomic treatment. This indicates that agronomic treatment can influence the microbial ecology of the rhizosphere in corn plants.

Experiments in Idaho evaluated genetic bacterial diversity and determined that the amount of microbial DNA extracted from soil correlated with soil C in a positive curvilinear relationship. Also, the amount of microbial DNA extracted from soil correlated with active bacterial biomass, active fungal biomass and active microbial biomass in positive curvilinear relationships.

A research project is underway in Puerto Rico, in cooperation with a farmer, to study the effect of herbicides on soil biomass. Preliminary data indicate that the most probable number (MPN) determination of Rhizobium is affected by the plant species that predominates in the plots. Further data on MPN populations in these plots will be continued.

Differential responses in citrus genotypes grown in Florida suggest that C expenditure on arbuscular mycorrhizal (AM) fungus - Glomus intraradices symbiosis is more tightly regulated in genotypes of low mycorrhizal dependency (MD), such as sweet orange, than in genotypes of high MD (e.g. sour orange). Further characterization of mycorrhizal effects on the carbon economy of citrus and the release of C from roots into the soil is planned. Also in Florida, research was conducted to test the level of functional diversity that exists among communities of AM fungi that are present in divergent Florida dunes. One study evaluated intra-location responses of plant and fungal genotypes on growth response of sea oats while a second study evaluated inter-location responses of plant and fungal ecotypes using the best combinations from experiment one. The research showed that there was a degree of specificity among hosts and their symbiotic fungi; however, relative to plant growth response, one fungal community (SG-1) was superior across plant ecotypes from all locations. Conversely, one plant ecotype (EK-17-3-2) tended to have the best response across all fungal communities.

Progress on a second edition of the textbook, Principles and Applications of Soil Microbiology, and a laboratory manual to accompany it, has continued.

Angle, JS, et al. 2001. Developing commercial phytoextraction technologies: Practical considerations. S Afr J Sci 97:619-623.

Chang, Y-J, et al. 2001. Impact of herbicides on abundance & structure of indigenous _-subgroup ammonia oxidizer communities in soil microcosms. Environ Toxicol Chem 20:2462-2468.

Delorme, T, et al. 2000. P accumulation by select plant species. J Phytoremediation. 2:344-349.

Gagliardi, JV, et al. 2001. Structural & functional analysis of whole-soil microbial communities for risk & efficacy testing following microbial inoculation of wheat roots in diverse soils. Soil Biol Biochem 33:25-40.

Haney, RL, et al. 2001. Molar concentration of K2SO4 & soil pH affect estima of extractable C with chloroform fumigation-extraction. Soil Biol Biochem 33:1501-1507.

Hartel, PG, et al. 2002. Biogeographic variability of E. coli ribotypes from ID & GA. J Environ Qual 31:1273-1278.

Jarstfer, AG, DM Sylvia. 2001. Isolation, culture & detection of arbuscular mycorrhizal fungi. p 535-542. Man of Environ Microbiol. 2nd Ed ASM Wash, DC.

Jifon, JL, et al. 2002. Growth depression of mycorrhizal citrus seedlings grown at high P supply is mitigated by elevated CO2. New Phytologist 153:133-142.

Kubikova, et al. 2001. Mycorrhizal impact on osmotic adjustment in Ocimum basilicum during a lethal drying episode. J Plant Physiol 158:1227-1230.

LaPara, TM, et al. 2001. Aerobic biolog treatment of pharmaceutical wastewater: Effect of temperature on COD removal & bacterial community development. Water Res 35:4417-4425.

Maldonado, JD, et al. 2001. Arbuscular mycorrhiza inoculum potential in natural & managed tropical montane soils in Costa Rica. Trop Agric 77:27-32.

Rickman, RW, et al. 2001. CQESTR: A model to estimate carbon sequestration in agric soils. J Soil Water Conser 56:237-246.

Schutter, ME, JJ Fuhrmann. 2001. Soil microbial community responses to fly ash amendment as revealed by analyses of whole soils & bacterial isolates. Soil Biol Biochem 33:1947-1958.

Sigler, WV, et al. 2001. Fate of biolog control agent Pseudomonas aureofaciens after application to turgrass. Appl Environ Microbiol 67:3542-3548.

Sylvia, DM, et al. 2001. Arbuscular mycorrhizal fungi influence tomato competition with bahiagrass. Biol Fertil Soils 34:448-452.

Sylvia, DM, DO Chellemi. 2001. Interactions among root-inhabiting fungi & their implications for biolog control of root pathogens. Adv Agron 73:1-33.

van Berkum, P, JJ Fuhrmann. 2001. Characteristics of soybean bradyrhizobia for which serogroup affinities have not been identified. Can J Microbiol 47:519-525.

Wagner, SC, et al. 2001. Long-term survival of Glomus claroideum propagules from soil pot cultures under simulated conditions. Mycologia 93:815-820.

Wilbur, JD, et al. 2001. Statistical issues in the analysis of microbial communities in soil. Proc Appl Stat in Agric. KSU, Manhattan, KS.

Wheeler, AL, et al. 2002. Potential of Enterococcus faecalis as a human fecal indicator for microbial source tracking. J Environ Qual 31:1286-1293.

Zuberer, DA 2001. N fixation (nonsymbiotic) in soils. Ency of Envir Micro. John Wiley & Sons, NY.

Becker, J, et al. 2001. Bacterial community diversity & activity at a mixed waste contaminated site. ASM Abst.

Bezdicek, D, M Fauci, S Albrecht. 2002. Soil carbon & C sequestration under different cropping & tillage practices in the Pacific NW. p 101-107 Proc NW Direct Seed Cropping Systems Conf. WA.

Booth, AM, C Hagedorn. 2001. Determining sources of fecal pollution in Blackwater River watershed, Franklin Co, Va. Crop & Soil Environ News. HTTP://www.ext.vt.edu/news/periodicals/cses/2001-11/franklincounty.html.

Bowman, AM, K Mentz, C Hagedorn. 2001. Determining sources of fecal pollution in Blackwater River, Franklin Co, VA. ASM Abst.

Bulinski, DA, et al. 2001. Rhizosphere microbial population dynamics over 3 field seasons. ASA Abst.

Carrero-Colsn, et al. 2001. Microbial community response to perturbation of a model wastewater treatment system. ASM Abst.

Chaney, RL, et al. 2001. Heavy metal aspects of compost use. Compost Utilization in Hort Cropping Syst. CRC Press, FL.

Curtis, P, CH Nakatsu, A Konopka. 2001. Acid tolerance of chemoheterotrophic bacteria isolated from an acidic (pH 3) soil. ASM Abst.

DeQueiroz, GA, et al. 2001. Viability & nodulation with Bradyrhizobium cultures after 10.5 years storage in sterile distilled water. ASA Abst.

Entry, JA, et al. 2001. Ribotyping of E. coli isolates from Rock Creek Watershed, ID. ASA Abst.

Entry, JA, RE Sojka. 2002. Polyacrylamide removes coliform bacteria from animal wastewater. p 1-3. Res & Ext Regional Water Qual Conf. WA.

Feng, Y, et al. 2001. Tillage effects on soil microbial community composition in continuous cotton syst. ASA Abst.

Frederick, JR, et al. 2001. Rhizobacteria from soybean & corn in rotation. ASA Abst.

Graham, JH, et al. 2002. Growth depression of mycorrhizal citrus seedlings grown at high P supply is mitigated by elevated CO2. 3rd Int Conf Mycorrhizas. Australia.

Gooden, DT, et al. 2001. Rhizobacteria from peanuts in rotation. ASA Abst.

Graves, AK, M Mahal, C Hagedorn. 2001. Determining sources of fecal pollution for a rural unsewered community. ASM Abst.

Hartel, PG, JD Summer, WI Segars. 2002. Potential for ribotyping to delist watersheds exceeding their TMDLs for fecal coliforms. Watershed Mgmnt to Meet Emerging TMDL Environ Regs. TX.

Hartel, PG. 2002. Ribotyping enterococci. p 11-15. Microbiol Source Tracking Wkshop. CA.

Hagedorn, C, et al. 2002. Carbon source utilization profiles as a method to identify sources of fecal pollution in water. ASM Abst.

Hagedorn, C. 2002. Carbon source profiles, pulsed-field gel electrophoresis & antibiotic resistance analysis. p 55-58. Microbiol Source Tracking Wkshop. CA.

Haines, SK, SP Deng. 2001. Isolation & characterization of bacteria capable of biodegrading explosives characterization of nitroaromatic-degrading bacteria isolated from explosive contaminated soils. ASA Abst.

Haney, RL, AJ Franzluebbers, FM Hons, DA Zuberer. 2001. Is K2SO4 extraction of microbial carbon pH dependent? ASA Abst.

Haney, RL, et al. 2001. Soil CO2 evolution: Is drying & rewetting substrate induced respiration? ASA Abst.

Haney, RL, et al. 2001. The flush of CO2: drying & rewetting vs. chloroform fumigation. ASA Abst.

Homhaul, W, J Fuhrmann, P van Berkum. 2001. Polyphasic analysis of soybean & cowpea bradyrhizobia isolated from US and Thai soils. ASA Abst.

Hons, F, J Thomas, D Zuberer. 2001. Chem, phys & biol properties of turfgrass soils. CD ROM. Soil & Crop Sci, Tx A&M U.

Jerke, K, CH Nakatsu, A Konopka. 2001. Genetic & physiological analyses of lead resistance in Arthrobacter sp. isolated from contaminated soil. ASM Abst .

Kirkpatrick, WD, PM White, Jr, GJ Thoma, EE Gbur, CM Reynolds, DC Wolf. 2001. Plant response to N addition in a crude oil-contaminated soil. ASA Abst.

Konopka, A, et al. 2001. Microbial community structure & function in a biomass-recycle bioreactor. 9th Int Symp Microbial Ecol. Netherlands.

Kuntz, RL, et al. 2002. Field-testing Enterococcus faecalis for microbial source tracking. ASM Abst.

Mesa, C, J Fuhrmann, P van Berkum. 2001. Phenotypic & genetic analysis of soybean bradyrhizobia isolated from DE soils. ASA Abst.

Motta, ACV, et al. 2001. Mgmnt systems to improve soil quality for cotton production on a degraded silt loam soil in AL (USA). p 219-222. Proc 1st World Cong on Conserv Agric. Spain.

Motta, ACV, et al. 2001. Enhancement of soil microbial biomass in cotton production systems with conserv tillage. 24th Ann So Conserv Tillage Conf Sust Agr.

Nakatsu, CH, J Joynt, A Konopka. 2001. Impact of metal & organic waste on a microbial community. 9th Int Symp Microbial Ecol. Netherlands.

Nakatsu, CH, et al. 2001. Chromium resistance by Arthrobacter sp. cr15 isolated from metal contaminated soil. ASM Abst.

Ravnskov, S, Y Wu, JH Graham. 2001. The influence of different arbuscular fungi & P on gene expression of invertase & sucrose synthase in roots of maize. 3rd Int Conf on Mycorrhizas. Australia.

Reynolds, CM, et al. 2001. Microbial populations during remediation at 3 petroleum-contaminated Alaskan sites. In Situ and On-Site Bioremediation. 6th Int Symp. CA.

Rommesser, J, JJ Fuhrmann. 2002. A method to assess quorum sensing potential of microbial communities. Pat (prov) Appl #60/346,531.

Thies, JE, T Marsh, C Nakatsu. 2002. Workshop Manual: Molecular Techniques for Analysis of Microbial Community Structure in Soil & the Rhizosphere. Cornell U, Ithaca, NY.

Thoma, GJ, et al. 2001. Mathematical modeling of phytoremediation of petroleum contaminated soils. 8th Ann Int Petro Environ Conf TX.

Thoma, GJ, et al. 2001. A mathematical model of phytoremediation of crude oil-contaminated soils. In Situ & On-Site Bioremediation. 6th Int Symp. CA.

Wang, G, HD Skipper. 2001. Denitrifying rhizobacteria from bentgrass & bermudagrass USGA greens. ASA Abst.

Wheeler, AL, et al. 2001. Ribotyping fecal streptococci to differentiate between human & nonhuman sources. ASA Abst.

White Jr, PM, et al. 2001. Field study to evaluate phytoremediation of crude oil-contaminated soil. ASA Abst.

White Jr, PM, WD Kirkpatrick, DC Wolf, GJ Thoma, RM Reynolds. 2001. Phytoremediation of crude oil-contaminated soil. 8th Ann Int Petro Environ Conf. TX.

Wilbur, JD, et al. 2001. A bayesian approach to modeling & variable selection in high-dimensional multivariate binary data. 2001 Joint Stat Mtgs.

Wilbur, JD, et al. 2001. Variable selection in high-dimensional multivariate binary data. New Eng Stat Symp.

Wu, G, Y Feng, SA Boyd. 2001. Effects of biphenyl-degrading bacteria characteristics on bioavailability of sorbed biphenyl. ASA Abst.

Zurdo-Piqeiro, JL, et al. 2002. Identificacisn de cepas de rhizobia que nodulan leguminosas tropicales en suelos de Puerto Rico. IX Nat Mtg on N Fixation. Spain.