SAES-422 Multistate Research Activity Accomplishments Report

Status: Approved

Basic Information

Participants

Baker, John [baker@anr.msu.edu] Michigan; Davis, Duane [davis@ksu.edu] Kansas; Diekman, Mark [mdiekman@purdue.edu] Indiana; Ford, Joe [joe.ford@ars.usda.gov] Nebraska (visitor); Guthrie, H. Dave [dave.guthrie@ars.usda.gov] USDA-BARC; Lents, Clay [clay.lents@ars.usda.gov] Nebraska (visitor); Levis, Donald [dlevis@unlnotes.unl.edu] Nebraska (visitor); Meisinger, David [drdave@iastate.edu] Iowa (visitor); Miles, Jeremy [jeremy.miles@ars.usda.gov] USDA-USMARC; Miller, Dave [djmille@illinois.edu] Illinois; Parrish, John [parrish@ansci.wisc.edu] Wisconsin; Rempel, Lea [lea.rempel@ars.usda.gov] USDA-USMARC (visitor); Ross, Jason [jwross@iastate.edu] Iowa; Safranski, Tim [safranskit@missouri.edu] Missouri; White, Brett [bwhite2@unlnotes.unl.edu] Nebraska; Wilson, Matt [mwilso25@wvu.edu] West Virginia;

The 2011 annual meeting of the NCERA057 was held at the United States Department of Agriculture Roman L. Hruska Meat Animal Research Center (MARC) in Clay Center, NE. The meeting was called to order by Jeremy Miles, Vice Chair and local host, at 8:00 AM on Tuesday, May 24th. Jeremy provided background information on the Meat Animal Research Center pertaining to research capabilities, scientists and personnel. Tuesday, May 24th, 2011 8 to 9:30 AM. We conducted a Swine Consultant Focus Group webinar to discuss research topic that address issues that are currently impacting the swine industry. The webinar was moderated by Dr. Don Levis. Consultants included in the webinar were Dr. Brad Belstra (Murphy Brown, LLC-Smithfield Premium Genetics, Rose Hill, NC), Dr. Steve Henry (Abilene Animal Hospital, Abilene, KS), Dr. Joe Connor (Carthage Veterinary Service, Carthage, IL), Dr. Paul Ruen (Fairmont Veterinary Clinic, Fairmont, MN), Dr. Larry Rueff (Swine Veterinary Service, Greensburg, IN) and Mr. Ron Ketchem (Swine Management Services, Fremont, NE). The consultant discussion focused on issues of concern that currently affect the swine industry and included: 1) tools for gilt development, 2) single insemination techniques, 3) expanding utilization of the top 10% of the boar population, 4) heat stress and seasonal infertility, 5) mitigation strategies during a PRRS virus challenge and others. The NCERA057 committee developed a list of these topics to be considered later in the meeting for symposium/workshop topics and for ideas to generate collaborative research projects. 9:30 to 10:00 AM. Dave Meisinger gave an update to the committee on the U.S. Pork Center of Excellence. This discussion detailed the current status of the Pork Information Gateway, Swine Science Online, and the current efforts of the Pork Center to develop an online troubleshooting guide that will be referred to as the National Swine Reproduction Guide and will be modeled after the National Swine Nutrition Guide. 10:00 to 10:30 AM. Adele Turzillo, National Program Leader for Animal Production Systems within the National Institute of Food and Agriculture (NIFA), addressed the committee via conference call. Adele provided an update on the structure of NIFA. It is composed of 4 Institutes of which each will employ a Principal Scientist for that institute. Notable items discussed were that the FY12 budget that was passed by the house possessed multiple, significant reductions in NIFA funding categories. She also informed us of Dr. Roger Beachys resignation and that NIFA leadership would in turn be restructuring. Regarding pre-and postdoc funding through NIFA, the committee was informed that the new contact is Margo Holland. 10:30 to 11:00 AM. Dr. John Pollack welcomed the NCERA057 committee to the MARC. Dr. Pollack described the animal facilities and expressed his encouragement for the development of strategic collaborations between members of the NCERA057 committee and faculty at the MARC. 11:00 to 11:30 AM. Jeremy Miles discussed the swine reproduction capabilities at MARC and introduced the investigators that are doing research relevant to swine reproduction. It was pointed out by Tim Safranski that the boar stud at MARC represents a unique facility that could be capitalized on from a research standpoint. Don Levis suggested the importance of maintaining valuable and unique germplasm. 11:30 to Noon. Matt Wilson led discussions on collaborative grants that could be written and submitted by members of the committee. Dave Meisinger suggested the willingness of the U.S. Pork Center of Excellence to contribute to grant writing efforts and interest in sow lifetime productivity research. Duane Davis suggested meeting more regularly to discuss grants, for example at the Midwest Animal Science sectional meetings in March. The committee categorized the areas of importance from the consultant discussion earlier in the day. The top nine areas of interest are: 1) Sow Productive Lifetime, 2) Number of AI services and AI technology, 3) Gilt Development/Predictions/checkpoints, 4) Sow housing, 5) Pig Quality/Survival, 6) Seasonal Infertility, 7) Mammary glands-milk quality/quantity, 8) Semen evaluation/strategy for contingency/ Management of backup boars, and 9) Virus mitigation. 12:30 to 12:45 PM. John Baker addressed the committee and praised the organization and accomplishments of the NCERA057 committee and indicated that this specific committee was among the best that he serves as an Administrative Advisor. He stated that the NCERA057 committee very accurately conducts the purposes intended for this program. Beginning at 12:45 PM. Station Reports: 1st Station Report: Dave Guthrie- USDA-ARS-Beltsville, MD, 2nd Station Report: Matt Wilson-West Virginia, 3nr Station Report: Mark Diekman-Purdue, 4th Station Report: John Parrish-Wisconsin, 5th Station Report: David Miller-Illinois, 6th Station Report: Tim Safranski-Missouri -Tim has identified a boar stud that is willing to provide aliquots of semen so that retrospective fertility data can be correlated to the data. Miller, Guthrie and Parrish offered ideas on how to process samples for storage. Simple protocols are necessary to provide to the boar stud. 3:30 to 5 PM. The committee was given a tour of the MARC animal facilities and outlying farms. Evening. The committee returned to the hotel for a social hour then reconvened at the home of Jeremy Miles for a barbecue hosted by Dr. Joe Ford (former US MARC representative to the NCERA057 committee). Wednesday, May 25th, 2011 8:00 to 9:00 AM. Began the business meeting. Dave Miller calls the meeting to order. It was determined that the 2012 meeting would be held at Iowa State University and hosted by Jason Ross. This will occur on Tuesday and Wednesday (May 22nd and 23rd, 2012). In addition, the 2012 meeting will deliver a producer symposium/workshop. It was determined that the symposium would be focused on AI and AI technology. The committee then discussed potential speakers for the workshop and the idea of having a discussion panel at the workshop. It was also noted that this was the 50th year anniversary of the NCERA057 committee. The 2013 meeting was determined to be held in Beltsville, Maryland. The new officers for the committee are: Jeremy Miles (new Chair), Jason Ross (new Vice Chair) and Dave Guthrie (new Secretary). It was then discussed that thank you letters be distributed to appropriate people for contributing to the meeting. Jason Ross agreed to write and send those documents. Dave Miller and Duane Davis agreed to write a new impact statement for the mid-term review. 9:00 to 10:00 AM. The committee began discussion on collaborative grants. Ideas included: utilization of frozen semen as a contingency with new AI technology, development of frozen semen delivery, ovulation control, and seasonal control of reproduction. The current plan is that Duane Davis, Dave Guthrie, Matt Wilson, Tim Safranski, Jeremy Miles and Lea Rempel will work out necessary preliminary data for an AFRI RFP. 10:00 AM to 12:00 PM: Station reports continued: 7th Station Report: Jason Ross- Iowa State, 8th Station Report: Duane Davis-Kansas State, 9th Station Report: Lea Rempel-MARC The meeting was adjourned at 12:05 PM. Respectfully submitted, Jason Ross, Secretary, NCERA057

Accomplishments

1. The committee members interacted with influential members of the swine industry during our consulting webinar that enabled the committee to be fully up to date on current reproductive issues facing the swine industry. 2. Numerous publications were published by committee members and their associates during the past year that provide scientific knowledge and impact on reproductive efficiency in swine. 3. The topic for the Biennial NCERA057 symposium was established based on the consultant group webinar. It was also established that this symposium would be conducted at Iowa State University. 4. A plan was established to write and submit proposals for funding in critical areas of swine reproductive physiology that are impacting the swine industry.

Impacts

  1. Continued developed teaching modules that will help institutions transition from traditional lecture- based animal science curriculum to online-based curriculum, including laboratories that focus on domestic animal reproduction.
  2. Characterized the functions of specific glycans in the oviduct that bind sperm to form a reservoir, which may alter sperm function and allow sperm to survive extended periods of time.
  3. Identified non-coding RNA molecules that impact cellular function in swine reproductive tissues.
  4. Refined systems for in vitro production and nuclear transfer of pig embryos.
  5. Characterization of the function of regulatory elements within the promoter region of the GnRH receptor by utilizing a variety of unique pig breeds and lines.
  6. Worked towards improving boar sperm cryopreservation techniques.
  7. Demonstrated the effects of sow exercise during gestation on placental function and fetal amino acids.
  8. Furthered development of estrus synchronization and utilization of aged semen.
  9. Mapping of QTL in the swine genome associated with reproductive traits.
  10. Characterization of piglet growth and blood components following reciprocal embryo transfer between different swine breeds.
  11. Developed associations between candidate genes and production traits in Landrace-Duroc-Yorkshire population.
  12. Improved understanding of mechanics of boar training for semen collection.
  13. Further developed utilization of Fourier harmonics to analyze collected semen and predict fertility.
  14. Developed a deeper understanding of the molecular mechanisms contributing to porcine oocyte function and embryo development.
  15. Improved the efficiency of isolating pig Wharton jelly derived stem cells and demonstrated their ability to migrate throughout the neonatal pig intestine.
  16. Advanced teaching techniques and strategies to improve education of animal science students.

Publications

Am-in N, Tantasuparuk W, Manjarin R, Kirkwood RN. Effect of site of sperm deposition on fertility to insemination with aged semen. J Swine Health Prod. 2011; (in press). Ansenberger K, Richards C, Zhuge Y, Barua A, Bahr JM, Luborsky J and Hales DB. 2010. Decreased severity of ovarian cancer and increased survival in hens fed a flaxseed enriched diet for one year. Gynecol. Oncology 117:341-347. Ashworth M.D., Ross J.W., Stein D., White F.J., Geisert R.D. Porcine endometrial Gene expression of acute phase extracellular matrix components following estrogen disruption of pregnancy. Animal Reproduction Science 2010; 122:215-221. Ashworth, M.D., Ross J.W., Stein D.R., DeSilva U., White F., Geisert R.D. Endometrial caspase 1 and interleukin-18 expression during the porcine estrous cycle and peri-implantation period of pregnancy and response to early exogenous estrogen administration. Reproductive Biology and Endocrinology 2010; 8:33. Bakst MR. Physiology and endocrinology symposium: Role of the oviduct in maintaining sustained fertility in hens. J Anim Sci. 2011; 89:1323-1329. Barb CR, Hausman GJ, Rekaya R, Lents CA, Lkhagvadorj S, Qu L, Cai W, Couture OP, Anderson LL, Dekkers JC, Tuggle CK. Gene expression in hypothalamus, liver and adipose tissues and food intake response to melanocortin-4 receptor (MC4R) agonist in pigs expressing MC4R mutations. Physiol Genomics 2010; 41-254-268. Barb, C.R., Hausman, G.J., Rekaya, R., Lents, C.A., Lkhagvadorj, S., Qu, L., Cai, W., Couture, O.P., Anderson, L.L., Dekkers, J.C.M., Tuggle, C.K. 2010. Gene expression in hypothalamus, liver, and adipose tissues and food intake response to melanocortin-4 receptor agonist in pigs expressing melanocortin-4 receptor mutations. Physiological Genomics 41(3):254-268. Barua A, Bitterman P, Bahr JM, Basu S, Sheiner E, Bradaric MJ, Hales DB, Luborsky JL, Abramowicz JS (2011). Contrast-enhanced sonography depicts spontaneous ovarian cancer at early stages in a preclinical animal model. J Ultrasound Med. 2011; 30(3):333-45 PMID: 21357555 Barua A, Bitterman P, Bahr JM, Bradaric M, Hales DB, Luborsky JL, and Abramowicz J. 2010. Ultection of tumor associated neo-angiogenesis by Doppler ultrasound during early stage ovarian cancer in laying hens: A preclinical spontaneous model of human ovarian cancer. Journal of Ultrasound in Medicine (JUM) 29:173-182. Barua A, Bitterman P, Bahr JM, Bradaric MJ, Hales DB, Luborsky JL, Abramowicz JS. (2010). Detection of tumor-associated neoangiogenesis by Doppler ultrasonography during early-stage ovarian cancer in laying hens: a preclinical model of human spontaneous ovarian cancer. J Ultrasound Med. 2010; 29(2):173-182. PMID: 20103787. Barua A, Bitterman P, Bahr JM, Vasu S, Sheiner e, Bradaric MJ, Hales DB, Luborsky JL, and Abramowicz JA. 2011. Contrast enhanced ultrasonography detects spontaneous ovarian cancer at early stage in a preclinical animal model. J. Ultrasound Med 30:333-345. Bauer BK, Isom SC, Spate LD, Whitworth KM, Spollen WG, Blake SM, Springer GK, Murphy CN, Prather RS. Transcriptional profiling by deep sequencing identifies differences in mRNA transcript abundance in in vivo-derived versus in vitro-cultured porcine blastocyst stage embryos. Biol Reprod. 2010 Nov;83(5):791-8. Blanco JM, Long JA, Gee G, Wildt DE, Donoghue AM. Comparative cryopreservation of avian spermatozoa: benefits of non-permeating osmoprotectants and ATP on turkey and crane sperm cryosurvival. Anim Reprod Sci. 2011; 123:242-248. Blomberg, L.A., Schreier, L.L., Guthrie, H.D., Sample, G.L., Vallet, J., Caperna, T., Ramsay, T. 2010. (Short Communication) The effect of intrauterine growth retardation on the expression of developmental factors in porcine placenta subsequent to the initiation of placentation. Placenta 31(6):549-552. Boltz CR, Belton RA, Boltz DA, Dirks A, Bunick D, Scherba G, Bahr JM. 2011. Vaccination of roosters against avian infectious bronchitis virus results in autoimmunity to sperm (submitted). Bradaric MJ, Barua A, Penumastsa K, Bitterman P, Abramowicz JS, Bahr JM, and Luborsky JL. 2011. Analysis of lymphocytes in spontaneous ovarian tumors in the laying hen show altered B cell content in early stage tumors. Journal of Ovarian Research (submitted). Bradaric MJ, Barua A, Penumatsa K, Yi Y, Edassery SL, Sharma S, Abramowicz JS, Bahr JM, Luborsky JL. (2011) Sphingosine-1 phosphate receptor (S1p1), a critical receptor controlling human lymphocyte trafficking, is expressed in hen and human ovaries and ovarian tumors. J Ovarian Res. 4(1):4-15. PMID: 21356044 Bradaric MJ, Barua A, Penumatsa K, Yi Y, Edassery SL, Sharma S, Abramowicz JS, Bahr JM, Luuborsky JL. 2011. Spingosine-1 phosphate receptor (S1P1), a critical receptor controlling human lymphocyte trafficking, is expressed in hen and human ovaries and ovarian tumors. J. Ovarian Res. 4:4. PMID 21356044. Braundmeier AG, Fazleabas AT, Nowak RA. Extracellular matrix metalloproteinase inducer (EMMPRIN) expression in the baboon endometrium: menstrual cycle and endometriosis. Reproduction 2010 140:911-920. Casas, E., Ford, J.J., Rohrer, G.A. 2010. Chap. 3. Quantitative genomics of male reproduction. In: Reproductive Genomics in Domestic Animals (Jiang, Z., Ott, T.L., eds.). Wiley-Blackwell Publishing, Ames, IA. pp. 53-66. Cepica, S., Bartenschlager, H., Ovilo, C., Zrustova, J., Masopust, M., Fernandez, A., Lopez, A., Knoll, A., Rohrer, G.A., Snelling, W.M., Geldermann, H. 2010. Porcine NAMPT gene: search for polymorphism, mapping and association studies. Animal Genetics 41(6):646-651. Checura CM, Beg MA, Parrish JJ, Ginther OJ. 2010. Positive effect of FSH but not LH on early development of the dominant follicle in mares. Reprod Fertil Dev. 2010;22(7):1092-9. Chen L, Bi J, Nakai M, Bunick D, Couse JF, Korach KS, Nowak RA. Expression of Basigin in reproductive tissues of estrogen receptor-alpha or beta null mice. Reproduction 2010 139:1057-1066. Cho SJ, Lee JS, Mathias ED, Chang C, Hickey GJ, Lkhagvadorj S, Anderson LL. Intracerebroventricular and intravenous administration of growth hormone secretagogue L-692,585, somatostatin, neuropeptide Y and galanin in pig: dose-dependent effects on growth hormone secretion. Comp Biochem Physiol C Toxicol Pharmacol. 2010; 151:412-9. D. Miller, T. Rathbun, D. Boyle, D. Troyer, and D. L. Davis. 2011. Confocal Imaging of Trans-epithelial Trafficking by Immune and Umbilical Cord Stem Cells in the Neonatal Porcine Intestine. In review. Dalloul RA, Long JA, Zimin AV, Aslam L, Beal K, Blomberg Le Ann, Bouffard P, Burt DW, Crasta O, Crooijmans RP, Cooper K, Coulombe RA, De S, Delany ME, Dodgson JB, Dong JJ, Evans C, Frederickson KM, Flicek P, Florea L, Folkerts O, Groenen MA, Harkins TT, Herrero J, Hoffmann S, Megens HJ, Jiang A, de Jong P, Kaiser P, Kim H, Kim KW, Kim S, Langenberger D, Lee MK, Lee T, Mane S, Marcais G, Marz M, McElroy AP, Modise T, Nefedov M, Notredame C, Paton IR, Payne WS, Pertea G, Prickett D, Puiu D, Qioa D, Raineri E, Ruffier M, Salzberg SL, Schatz MC, Scheuring C, Schmidt CJ, Schroeder S, Searle SM, Smith EJ, Smith J, Sonstegard TS, Stadler PF, Tafer H, Tu ZJ, Van Tassell CP, Vilella AJ, Williams KP, Yorke JA, Zhang L, Zhang HB, Zhang X, Zhang Y, Reed KM. Multi-platform next-generation sequencing of the domestic turkey (Meleagris gallopavo): genome assembly and analysis. PLoS Biol. 2010 Sep 7;8(9). pii: e1000475. Dharmendra Kumar Maurya, Chiyo Doi, Marla Pyle, Raja Shekar Rachakatla, Duane Davis, Masaaki Tamura, and Deryl Troyer. 2011. Non-random tissue distribution of human naïve umbilical cord matrix stem cells. World J Stem Cells 2011 April 26; 3(4): 34-42. Dobbs KB, Spollen WG, Springer G, Prather RS.The role of cytoplasmic polyadenylation element sequence on mRNA abundance during porcine embryogenesis and parthenogenetic development. Mol Reprod Dev. 2010 Aug;77(8):699-709. Flowers, W.L. 2010. Biotechnology: Artificial Insemination. In: Encyclopedia of Agricultural Biotechnology. M. Wheeler (eds.), Marcell Dekker, Inc., New York, 457-460. Flowers, W.L. 2010. Trouble shooting reproductive problems. 2010. Proceedings, Banff Pork Seminar. http://www.banffpork.ca/proceedings.cgi. Ford, J.J., Wise, T.H. 2011. Assessment of pubertal development of boars derived from ultrasonographic determination of testicular diameter. Theriogenology 75(2):241-247. Grudzien MM, Low P, Manning PC, Arredondo M, Belton Jr. RJ, Nowak RA. The antifibrotic drug halofuginone inhibits proliferation and collagen production by human leiomyoma and myometrial smooth muscle cells. Fertility and Sterility 2010 93:1290-1298. Guthrie HD, Welch GR, Theisen DD, and Woods LC III. 2011. Effects of liquid hypothermic storage, freezing rate, and extender type on viability, calcium homeostasis, and ATP content in captive striped bass (Morone saxatilis) sperm. Abstract accepted for presentation as a poster at the 44th Annual meeting of the Society for the Study of Reproduction (SSR) held in Portland, Oregon, July 31- August 4, 2011. Guthrie HD, Welch GR, Theisen DD, Woods LC III. Effects of hypothermic storage on intracellular calcium, reactive oxygen species formation, mitochondrial function, motility, and plasma membrane integrity in striped bass (Morone saxatilis) sperm. Theriogenology. 2011 75:951-961. Heaton, M.P., Leymaster, K.A., Kalbfleisch, T.S., Freking, B.A., Smith, T.P.L., Clawson, M.L., Laegreid, W.W. 2010. Ovine reference materials and assays for prion genetic testing. BMC Veterinary Research 6:23. Hughes PE, Smits RJ, Kirkwood RN. Relationships among gilt and sow live weight, P2 backfat depth and culling rates. J Swine Health Prod. 2010; 18:301-305. Isom SC, Spollen WG, Blake SM, Bauer BK, Springer GK, Prather RS. Transcriptional profiling of day 12 porcine embryonic disc and trophectoderm samples using ultra-deep sequencing technologies. Mol Reprod Dev. 2010 Sep;77(9):812-9. Keating AF, Sen N, Sipes IG, Hoyer PB. Dual protective role for glutathione S-transferase class pi against VCD-induced ovotoxicity in the rat ovary. Toxicol Appl Pharmacol. 2010; 247:71-5. Knox, R. V., K. L. Willenburg, S. L. Rodriguez-Zas, D. L. Greger, H. D. Hafs, and M.E. Swanson. Synchronization of ovulation and fertility in weaned sows treated with intravaginal triptorelin is influenced by timing of administration and follicle size. Theriogenology 2011. 75:308-19. Konno, T., Graham, A.R., Rempel, L.A., Ho-Chen, J.K., Alam, S.M.K., Bu, P., Rumi, M.A.K., Soares, M.J. 2010. Subfertility linked to combined luteal insufficiency and uterine progesterone resistance. Endocrinology 151(9):4537-4550. Lee, K, Wang, C, Chaille, JM, and Machaty, Z. 2010. Effect of resveratrol on the development of porcine embryos produced in vitro. J. Reprod. Dev. 2010; 56:330-335. Lemley CO, Vonnahme KA, Tager LR, Krause KM, Wilson ME. Diet-induced alterations in hepatic progesterone (P4) catabolic enzyme activity and P4 clearance rate in lactating dairy cows. J Endocrinol. 2010 Jun;205(3):233-41. Lin, L, Pribenszky, C, Molnár, M, Kragh, PM, Du, Y, Zhang, X, Yang, H, Bolund, L, Callesen, H, Machaty, Z, and Vajta, G. 2010. High hydrostatic pressure treatment of porcine oocytes induces parthenogenetic activation. Cell. Reprogram. 2010; 12:475-480. Lindholm-Perry, A.K., Rohrer, G.A., Kuehn, L.A., Keele, J.W., Holl, J.W., Shackelford, S.D., Wheeler, T.L., Nonneman, D.J. 2010. Genomic regions associated with kyphosis in swine. BMC Genetics 11:112. Lkhagvadorj S, Qu L, Cai W, Couture OP, Barb CR, Hausman GJ, Nettleton D, Anderson LL, Dekkers JC, Tuggle CK. Gene expression profiling of the short-term adaptive response to acute caloric restriction in liver and adipose tissues of pigs differing in feed efficiency. Am J Physiol Regul Integr Comp Physiol. 2010; 298:R494-507. Machado, SA, Bahr JM, Hales DB, Quade BJ, and Nowak RA. 2011. Validation of the aging hen as an animal model for uterine leiomyomas. Biology of Reproduction (submitted). Mark-Kappeler CJ, Sen N, Keating AF, Sipes IG, Hoyer PB. Distribution and responsiveness of rat anti-Müllerian hormone during ovarian development and VCD-induced ovotoxicity. Toxicol Appl Pharmacol. 2010; 249:1-7. Masopust, M., Vykoukalova, Z., Knoll, A., Bartenschlager, H., Mileham, A., Deeb, N., Rohrer, G.A., Cepica, S. 2011. Porcine insulin receptor substrate 4 (IRS4) gene: cloning, polymorphism and association study. Molecular Biology Reports 38(4):2611-2617. Mesquita FS, Dyer SN, Heinrich DA, Bulun SE, Marsh EE, Nowak RA. Reactive oxygen species mediate mitogenic growth factor signaling pathways in uterine smooth muscle cells. Biology of Reproduction 2010 82:341-351. Miller DJ. 2011. Physiology and Endocrinology Symposium: Sperm-oviduct interactions in livestock and poultry. J Anim Sci 89:1312-1314. Miller DJ. 2011. The enigma of fertilization. In: Biology: The Dynamic Science, 2nd Edition, Ed. by Peter Russell, Stephen Wolfe, Paul Hertz, Cecie Starr, and Beverly McMillan. In Press. Miller DJ. 2011. The roles of carbohydrate binding in fertilization. In: Carbohydrate Recognition: Biological Problems, Methods, and Applications, Ed. By B Wang and G-J Boons. Medicinal Research Reviews. In Press. Moore K, Lovercamp KW, FenD, Antelman J, Sutovsky M, Manandhar K, van Leyen, Safranski T, Sutovsky P. 2010. Altered epididymal spem maturation and cytoplasmic droplet migration in subfertile male Alox15 mice. Cell Tissue Res. 340:569-581. Murack, PJ. Parrish, JJ. Barry, TP. (2011) Effects of progesterone on sperm motility in fathead minnow (Pimephales promelas). Aquatic Toxicology. In Press Newell-Fugate, A,E Taibl, JN, Clark, SG, Alloosh, M, Sturek, M, Krisher, RL. 2011. Baseline and superovulation hyperandrogenism and follicular dynamics in the ossabaw pig suggest an animal model for polycystic ovary syndrome (PCOS). Reproduction, Fertility and Development. 23(1): 256-257. Nguyen K, Cassar G, Friendship RM, Dewey C, Farzan A, Kirkwood RN. Stillbirth and preweaning mortality in litters of sows induced to farrow with supervision compared to litters naturally farrowing sows with minimal supervision. J Swine Health Prod. 2011; (in press) Nowak RA, Koohestani F, Bi J, Mehrotra P, Mesquita FS, Masoud F, Machado SM. Uterine Tumors and the Environment. In: Comprehensive Toxicology, ed. by CA McQueen, Section IV Female Reproductive Toxicology- Female Reproductive C: 2010;499-522. Oliver, W.T., Miles, J.R. 2010. A low-fat liquid diet increases protein accretion and alters cellular signaling for protein synthesis in 10-day-old pigs. Journal of Animal Science 88(8):2576-2584. Park, KE, Johnson, C, Magnani, L, Wang, X, Biancardi, M, and Cabot, RA. 2010. Global H3K9 dimethylation status is not affected by transcription, translation or DNA replication in porcine zygotes. Molecular Reproduction and Development, 77(5):420-429. Park, KE, Johnson, CM, Wang, X, and Cabot, RA. 2010. Differential developmental requirements for individual histone H3K9 methyltransferases in cleave stage porcine embryos. Reproduction, Fertility and Development, In press. Parrish JJ. 2010. Biology and physiology of fertilization. In: Reproductive Toxicology Third Edition, Target Organ Toxicology Series. (Hayes AW, Thomas JA, Gardner DE eds.) pp. 1-13. Peláez J, Bongalhardo DC, Long JA. Characterizing the glycocalyx of poultry spermatozoa: III. Semen cryopreservation methods alter the carbohydrate component of rooster sperm membrane glycoconjugates. Poult Sci. 2011;90:435-443. Rahman MB, Vandaele L, Rijsselaere T, Maes D, Hoogewijs M, Frijters A, Noordman J, Granados A, Dernelle E, Shamsuddin M, Parrish JJ, Van Soom A. 2011 Scrotal Insulation and Its Relationship to Abnormal Morphology, Chromatin Protamination and Nuclear Shape of Spermatozoa in Holstein-Friesian and Belgian Blue Bulls. Theriogenology (accepted). Rempel, L.A., Freking, B.A., Miles, J.R., Nonneman, D.J., Rohrer, G.A., Schneider, J.F., Vallet, J.L. 2011. Association of porcine heparanase and hyaluronidase 1 and 2 with reproductive and production traits in a Landrace-Duroc-Yorkshire population. 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