SAES-422 Multistate Research Activity Accomplishments Report

Status: Approved

Basic Information

Participants

Meeting minutes includes list of those present. URL: https://catalysttools.washington.edu/workspace/sr320/8608/41770

Accomplishments

" Increased level of awareness among participants of the problems facing West coast shellfish and hatcheries in dealing with the effects of coastal upwelling and acidification of coastal waters. " Learned from international participants about strategies to transfer selected broodstock and information to industry " New collaborative projects have been developed to address the impacts of ocean acidification on West coast shellfish " Dr. Reece served on the Oyster Restoration Workshop Panel for the Hudson River Foundation, Aug. 11-12, 2009, NYC, New York. " During this past year the Reece lab continued development and implementation of genetic markers for the hard clam Mercenaria mercenaria

Impacts

  1. Establishment of a database dedicated to C. gigas EST (Fleury et al., BMC Genomics 2009, 10: 341).
  2. First use of RNA interference in C. gigas, demonstrating the role of vasa in germ cell development (Fabioux et al., FEBS journal 276: 2566-2573)
  3. QTL for resistance to: - summer mortality and OsHV-1 load in C. gigas (Sauvage et al., Animal Genetics, in press) - bonamiosis in the European flat oyster O. edulis (Lallias et al., Marine Biotechnology 11: 570584).
  4. Genetic identification of oyster samples found lose in the Coan River. Oysters confirmed to be triploid C. ariakensis. Report sent to VIMS Dean/Director, VIMS DRAS and VMRC.
  5. Genetic identification of oysters found on crab pots in the York River. Oysters confirmed to be C. virginica and not C. ariakensis as suspected. March 2009. Report sent to VIMS Dean/Director, VIMS DRAS and VMRC.

Publications

Publications for the current year only. Batista FM, Boudry P, Dos Santos A, Renault T, Ruano F (2009). Infestation of the cupped oysters Crassostrea angulata, C. gigas and their first generation hybrids by the copepod Myicola ostreae: differences in susceptibility and host response. Parasitologia 136 (5): 537-543. Sauvage C, Pépin JF, Lapègue S, Boudry P, Renault T (2009). Ostreid herpes virus infection in families of the Pacific oyster, Crassostrea gigas, during a summer mortality outbreak: differences in viral DNA detection and quantification using real-time PCR. Virus Research, 142: 181187 Sauvage C, Boudry P, Lapègue S. Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas. Molecular Ecology Notes, 9(3): 853-855. Lallias D, StockdaleR, Boudry P, Beaumont AR, Lapègue S. Characterization of 27 microsatellite loci in the European 1 flat oyster Ostrea edulis. Molecular Ecology Resources, 9(3): 960-963. Lallias D, Gomez-Raya L, Haley CS, Arzul I, Heurtebise S, Beaumont AR, Boudry P, Lapègue S (2009). Combining two-stage testing and interval mapping strategies to detect QTL for resistance to bonamiosis in the European flat oyster Ostrea edulis. Marine Biotechnology 11:570584. Lallias D, Stockdale R, Boudry P, Lapègue S, Beaumont A (2009). Characterization of ten microsatellite loci in the blue mussel Mytilus edulis. Journal of Shellfish Research 28(3): 547-551. Fleury E, Huvet A, Lelong C, de Lorgeril J, Boulo V, Gueguen Y, Bachère E, Tanguy A, Moraga D, Fabioux C, Lindeque P, Shaw J, Reinhardt R, Prunet P, Davey G, Lapègue S, Sauvage C, Corporeau C, Moal J, Gavory F, Wincker P, Moreews F, Klopp C, Mathieu M, Boudry P, Favrel P (2009). Generation and analysis of a 29,745 unique Expressed Sequence Tags from the Pacific oyster (Crassostrea gigas) assembled into a publicly accessible database: the GigasDatabase. BMC Genomics, 10: 341. Arias A, Freire R, Boudry P, Heurtebise S, Méndez J, Insua A (2009). Single nucleotide polymorphism for population studies in the scallops Aequipecten opercularis and Mimachlamys varia. Conservation Genetics, 10:14911495. Cannuel R, Beninger PG, McCombie H, Boudry P (2009). Gill development, functional and evolutionary implications in the blue mussel Mytilus edulis (Bivalvia: Mytilidae). Biological Bulletin 217: 173-188. Normand J, Ernande B, Haure J, McCombie H, Boudry P (2009). Reproductive effort in Crassostrea gigas: comparison of 5-month-old diploid and triploid oysters issued from natural crosses or chemical induction. Aquatic Biology 7(3): 229-241 Taris N, Boudry P, Bonhomme F, Camara MD, Lapègue S (2009). Mitochondrial and nuclear DNA analysis of genetic heterogeneity in temporal recruitment cohorts of the European flat oyster, Ostrea edulis. The Biological Bulletin 217(3): 223-241 Dégremont L, Bédier E, Boudry P (2010). Summer mortality of hatchery-produced Pacific oyster spat (Crassostrea gigas). II. Response to selection for survival and its influence on growth and yield. Aquaculture 299: 21-29. Dégremont L, Boudry P, Ropert M, Samain JF, Bédier E, Soletchnik P (2010). Effects of age and environment on survival of summer mortality by two selected lines of the Pacific oyster Crassostrea gigas. Aquaculture 299: 44-50. Fleury E, Moal J, Boulo V, Daniel J-Y, Mazurais D, Hénaut A, Boudry P, Favrel P, Huvet A. Identification of new genes associated with summer mortality in the oyster Crassostrea gigas. Marine Biotechnology, in press. Pernet F, Barret J, Marty C, Moal J, Le Gall P, Boudry P. Environmental anomalies, energetic reserves and fatty acid modifications in oysters coincide with an exceptional mortality event. Marine Ecology Progress Series, in press. Sauvage C, Boudry P, de Koning DJ, Haley CS, Heurtebise S, Lapègue S. Quantitative Trait Loci for resistance to summer mortality and OsHV1 load in the Pacific oyster (Crassostrea gigas). Animal Genetics, in press. Huvet A, Normand J, Fleury E, Quillien V, Fabioux C, Boudry P. Reproductive effort of Pacific oysters: a trait associated with susceptibility to summer mortality. Aquaculture, in press. Geay F, Santigosa I Culi E, Corporeau C, Boudry P, Dreano Y, Corcos L, 4, Bodin N, Vandeputte M, 7, Zambonino-Infante JL,Mazurais D, Cahu CL. Regulation of FADS2 expression and activity in European sea bass (Dicentrarchus labrax, L.) fed a vegetable diet. Comparative Biochemistry and Physiology, in press. Lallias D, Boudry P, Lapègue S, King JW, Beaumont AR. Strategies for the retention of high genetic variability in European flat oyster (Ostrea edulis) restoration programmes. Conservation Genetics, in press. Lang, P.L., Bayne, C.J, Camara, M.D., Cunningham, C., Jenny, M.J., and Langdon C.J. 2009. Transcriptome profiling of selectively-bred Pacific oyster Crassostrea gigas families that differ in tolerance of heat shock. Marine Biotechnology, 11: 650-668 Lang, R.P., Langdon, C.J., Taris, N.G. and M.D. Camara. Can laboratory assays be used to predict field performance of Pacific oyster (Crassostrea gigas) families planted in coastal waters? Aquaculture, accepted. Costa de Melo, A.G., Gardunho, D.C.L., Schneider, H., Sampaio, I., Gaffney, P.M., Reece, K.S., Beasley, C.R. and C.H. Tagliaro. (in press) Molecular identification, phylogeny and geographic distribution of Brazilian mangrove oysters (Crassostrea). Gen. Mol. Biol. Xiao, J. Cordes, J.F., Wang, H., Guo, X., and K.S. Reece. (in press) Population genetics of Crassostrea ariakensis in Asia inferred from microsatellite markers. Mar. Biol. Roberts SB, Goetz G, White S, Goetz F (2009) Analysis of genes isolated from plated hemocytes of the Pacific Oyster, Crassostrea gigas. Marine Biotechnology. 11:24-44 DeFaveri J, Smolowitz R, Roberts, S. (2009) Development and validation of a real-time quantitative PCR assay for the detection and quantification of Perkinsus marinus in the Eastern oyster, Crassostrea virginica. Journal of Shellfish Research Books and books chapters Boudry P (2009) Genetic variation and selective breeding in hatchery-propagated molluscan shellfish. In Burnell G & Geoff A (eds): New technologies in aquaculture: Improving production efficiency, quality and environmental management. Woodhead Publishing Limited, Cambridge, U.K, pp. 87-108. Beaumont A, Boudry P, Hoare K (2010). Biotechnology and genetics in Fisheries and Aquaculture (2nd ed.). Wiley-Blackwell, Chichester, UK. 202 p.
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