Brief Summary of Minutes of Annual Meeting

Peng Chee provided background of the project. Collaborative project among primarily State Universities.

1. Wayne Smith was previous chair, Gerald Myers previous secretary.

2. Peng Chee is current chair, Osman A. Gutierrez current secretary.

3. Clarence Watson is current advisor.

4. Four goals of the project were provided.

1. Association mapping of David Weaver reniform nematode and fiber properties using racestock accessions from the US collection.
2. Drought tolerance gene expression.
3. Salt tolerance in tetraploids. G. hirsutum has best tolerance at a very young stage. The screen includes Paraguay, DP33B (NuCotton 33B) and G. tomentosum.

1. Create a HapMap in cotton and map complex traits by developing populations that will be available to the community.

1. Breeding, genetics, and genomics program.
2. Improve fiber quality in Acala gene pool.
3. Working with a Pima breeding program.
4. Fusarium Wilt Race 4 resistance work and combining other Fusarium resistance
5. Nematode genetics - chromosome 11 with 174 BACS identified, 15 of which are sequenced to identify SNPs.
6. Developing a consensus map with John Yu.

1. Interspecific introgression from G. mustelinum using advanced backcross approach.
2. F2 map for G. hirsutum x G. mustelinum: 1000 loci covering all 26 chromosomes.
3. ab-qtl analysis of bc3f2 family fiber quality.

1. Association mapping project using AFLPs in coordination with Fred Bourland using true breeding lines.
2. Also using materials submitted to the RBTN.
3. Heterosis work.
4. QTLs for oil and protein.
5. Routine breeding efforts and Root Knot evaluation.

1. LONREN work. Evaluating F2 and F2:3 lines in the field. ARS-Stoneville is conducting marker work.
2. Traditional breeding program moving forward.

1. Continuing to make selections for LONREN (marker), root knot (marker), and nectarless (ne) traits.

1. Random mating population was released in 2007 and it is freely available by request to Dr. Jenkins.

1. Molecular cytogenetics, wide hybridization, chromosome biology, chromosome pairing studies.
2. Studying hybrid vigor-basic and applied, leaf morphology related to boll rot - both molecular and conventional.
3. Thrips tolerance from G. barbadense, earliness.
4. Started a small association mapping project

1. Acala (90%) and Pima breeding (10%). Tying to develop Acala germplasm with Pima quality.
2. Drought tolerance QTL research looks promising.
3. Microarray to identify drought responsive genes some have been confirmed via quantitative PCR.
4. Verticillium wilt resistance field test on 146 advanced breeding lines from interspecific cross.

1. New institute at OSU.
2. Translational genomics using Arabidopsis (AtA20 over expressed gene).
3. Cotton transgenic show increased dessication tolerance.
4. LOS2 transcription factor related to drought tolerance.
5. Promoter selection is critical.

1. Continuing an upland germplasm enhancement focused on fiber quality.
2. A Paper (In Press) regarding marker and trait based diversity of Pee Dee germplasm with the emphasis being to provide community information to help them select specific Pee Dee lines as parents.
3. Continued development of chromosome specific RIL pops for chromosome 10 and chromosome 12sh.
4. Preliminary evaluation of 300 hirsutum race stock accessions.
5. Molecular work ongoing to identify aquaporin gene family in cotton and will soon submit the first manuscript.

1. Released 8 ELS germplasm lines last year, proposed 1 additonal this year that has improved lint percent; also another proposed release with fiber strength in mid 30s.
2. Spinning properties of ELS lines in association with Eric Hequet.
3. Genetics of ELS and elite fiber quality.
4. Multiple non-ELS lines being evaluated for release; 1 is okra leaf.

1. Fiber maturity in G. arboreum, G. herbaceum, and G. hirsutum.
2. Auxin applications.
3. Relating HVI and AFIS properties
4. G. hirsutum x G. barbadense Sea Island introgressions.
5. Took a group of students to Tecomán, Mexico to help with USDA characterization efforts.
6. Leaf temp drought tolerance.

1. Cotton cytogenetic collection: development of CSL.
2. Presented summary of cytogenetic stocks for hirsutum.
3. Presented Chromosome Substitution Lines available for G. barbadense, G. tomentosum, G. mustelinum.
4. Line performance per se, F2s, topcrosses, intercrosses, cs-rils: recommended community to focus on utilizing these resources to compete for new funding sources.
5. Wide cross introgression involving complex populations.
6. Reniform nematode collaborations.
7. Whole genome radiation hybrid mapping: structural genomics.
8. Development of SNPs.
9. Generating lots of DNA sequence using high throughput sequencing and assembling those sequences. Difficulty is validating and mapping putative SNPs.
10. NSF fiber cell development project.

1. Using the fiber quality and short season TAES Lubbock germplasm pool to work on fiber property measurements.
2. Breeding program continues on to improve fiber quality of adapted germplasm.
3. Continue working on screening for abiotic stress, etc.

1. Genetic map, physical map, and transcriptome map EST Unigenes.
2. Application for germplasm characterization, QTL identification, and association mapping with Uzbek.

1. Genetic variability within USDA collection.
2. Four germplasm pool research dwarf, fiber quality, race stock.
3. Fiber quality traits.
4. Subset of G. hirsutum from Africa, China, US current, US obsolete: looking for genetic potential for breeding and molecular genetic diversity.

1. TAG paper published for diploid bacterial blight research.
2. Crop Science publication looking at transgenic cotton expressing several abiotic stresses in collaboration with Randy Allen.

1. Develop BAC resources for community from TM-1, 8X coverage.
2. Fiber genomics pro