Brief Summary of Minutes of Annual Meeting

see attached

1. Resistance management of pest insect species represents a serious challenge to corn growers that utilize both transgenic corn varieties as well as conventional pesticides. Research conducted in Nebraska provides valuable tools for effective and long-term pest management. 2. Development of resistance monitoring tools for German cockroaches in Florida to identify evolving physiological and behavioral resistance to gel baits. 3. Identification of negative cross-resistance factors in flies and mosquitoes. 4. Understanding resistance evolution to new insecticide chemistries in houseflies. 5. Development of resistance monitoring and management tools for chinch bugs in turf. 6. Development of resistance management programs for chili thrips in urban landscapes. 7. Continued monitoring of existing organophosphate resistance in California red scale reminds growers to rotate insecticides to manage resistance. 8. Monitoring for Esteem resistance in California red scale reduces wasted applications when resistance becomes significant. 9. Monitoring for organophosphate resistance in citricola scale will reduce organophosphate insecticide treatments where resistance is a problem. This reduction will improve air quality, water quality and reduce VOCs. 10. Expansion of a multi-state cooperative migration and resistance monitoring network, allowed maximum leveraging of limited public and private-sector funds, and allowed all participants to benefit of the early warning system capabilities of the complete system, via knowledge of CEW flights in nearby states. Due to erratic, yet continued control problems with the pyrethroids in sweet corn, efficacy and resistance monitoring data was shared among many of the Midwest states to develop a Section 18 emergency request for Coragen (Rynaxypyr), DuPont (request pending). A series of 7 CEW IRM papers were published in 2007, resulting from a symposium in 2005. 11. A bioassay conducted in spring squash crops in NY and PA yielded information on fungicide sensitivity in the cucurbit powdery mildew fungus that was used to guide fungicide recommendation for managing powdery mildew in pumpkin, melon, and other main season crops. 12. Through a baseline sensitivity study, the cucurbit powdery mildew fungus was not found to be more sensitive as anticipated to new DMI (FRAC code 3) fungicides. 13. Fungicides are by far the most effective option for managing gummy stem blight, one of the most widespread and destructive diseases of watermelon. Research conducted in Georgia has provided evidence of widespread resistance of the pathogen to the QoI fungicide azoxystrobin in Georgia and the first report of resistance of this pathogen to the carboxamide fungicide boscalid. These results, along with continued sensitivity monitoring and evaluation of fungicide efficacy, provide important information for watermelon growers, extension specialists, and county agents, for developing specific fungicide programs for effective management of gummy stem blight. 14. Research to improve our understanding of the mechanisms of herbicide resistance in weedy species is adding significant insight to our knowledge about plant responses to severe abiotic stresses, and also our understanding of auxin binding and auxin-mediated responses.

Brock, J., Stevenson, K., and Brenneman, T. 2007. Pecan fungicides and resistance management. Pages 172-175 in: Southeastern Pecan Growers' Handbook. Bulletin 1327. Cooperative Extension Service, University of Georgia College of Agricultural and Environmental Sciences. Kaufman, P. E., Gerry, A. C., Rutz, D. A. and Scott, J. G. 2007. Monitoring susceptibility of house flies (Musca domestica L.) in the United States to imidacloprid. J. Agric. Urban Entomol. 23: 195-200. Gao, J.-R., Deacutis, J. M. and Scott, J. G. 2007. Characterization of the nicotinic acetylcholine receptor subunits Mda5 and Mdb3 on autosome 1 of Musca domestica indicate they are not involved in spinosad resistance. Insect Molec. Biol. 16: 691-701. Hardstone, M.C., Leichter, C.A., Harrington, L.C., Kasai, S., Tomita, T. and Scott, J. G. 2007. Cytochrome P450 monooxygenase-mediated permethrin resistance confers limited cross-resistance in larvae of the southern house mosquito, Culex pipiens quinquefasciatus. (89: 175-184). Deacutis, J. M., Leichter, C. A., Gerry, A. C., Rutz, D. A., Watson, W. D., Geden, C. J. and Scott, J. G. 2007. Susceptibility of field collected house flies to spinosad before and after a season of use. J. Agric. Urban Entomol. 23: 105-110. Rinkevich, F. D., Hamm, R. L., Geden, C. J. and Scott, J. G. 2007. Dynamics of insecticide resistance alleles in two different climates over an entire field season. Insect Biochem. Molec. Biol. 37: 550-558. Gao, J.-R., Deacutis, J. M. and Scott, J. G. 2007. The nicotinic acetylcholine receptor subunit gene Mda6 from Musca domestica is diversified via post transcriptional modification. Insect Molec. Biol. 16: 325-334. Gao, J.-R., Kozaki, T., Leichter, C. A., Rinkevich, F. D., Shono, T., and Scott, J. G. 2007. The A302S mutation in Rdl that confers resistance to cyclodienes and limited cross-resistance to fipronil is undetectable in field populations of house flies from the USA. Pestic. Biochem. Physiol. 88: 66-70. Gao, J.-R., Deacutis, J. M. and Scott, J. G. 2007. The nicotinic acetylcholine receptor subunit gene Mdalpha2 from the housefly, Musca domestica. Arch. Insect Biochem. Molec. Biol. 64: 30-42. Ozoe, Y., Ishikawa, S., Tomiyama, S., Ozoe, F. and Scott, J. G. 2007b. Antagonism of the GABA receptor of dieldrin-resistant houseflies by fipronil and its analogues. In Synthesis and Chemistry of Agrochemicals Series VII, J. W. Lyga and G. Theodoritis, ed. Amer. Chem Soc., Washington, DC, pp 39-50. Nguyen, S.N., C. Song and M.E. Scharf. 2007. Toxicity, synergism and neurological effects of novel volatile insecticides to insecticide-susceptible and -resistant Drosophila strains. J. Econ. Entomol. 100(2): 534-544. Buss, E. A., J. F. Price, E. McCord, and C. Nagle. 2007. Managing insecticide and miticide resistance in Florida landscapes. University of Florida, IFAS, ENY-842. 10 pp. Funderburk, J., Diffie,S., Sharma, J., Hodges, A. and Osborn, L. 2007. Thrips of ornamentals in the Southeastern U.S. University of Florida, IFAS, ENY-845 (IN754). 10 pp. Crowder, D. W., C. Ellers-Kirk, B. E. Tabashnik, and Y. Carrière. Lack of fitness costs associated with pyriproxyfen resistance in the B biotype of Bemisia tabaci. Pest Manag. Sci., submitted. Crowder, D. W., A. R. Horowitz, B. E. Tabashnik, T. J. Dennehy, I. Denholm, K. Gorman, and Y. Carrière. Analyzing haplodiploid inheritance of insecticide resistance in whitefly biotypes. Bull. Entomol. Res, in revision. Crowder, D.W., P.C. Ellsworth, B.E. Tabashnik and Y. Carriere. 200-. Effects of operational factors on evolution of resistance to pyriproxyfen in the sweetpotato whitefly (Hemiptera: Aleyrodidae). Environmental Entomology, in press. Crowder, D. W., C. Ellers-Kirk, C. Yafuso, T. J. Dennehy, B. A. Degain, V. S. Harpold, B. E. Tabashnik, and Y. Carrière. 2008. Inheritance of resistance to pyriproxyfen in Bemisia tabaci (Hemiptera: Aleyrodidae) males and females (B biotype). J. Econ. Entomol. 101: 927-932. Crowder, D.W., T.J. Dennehy, C. Ellers-Kirk, C.M. Yafuso, P.C. Ellsworth, B.E. Tabashnik & Y. Carrière. 2007. Field evaluation of resistance to pyriproxyfen in Bemisia tabaci (B Biotype). Journal of Economic Entomology 100: 1650-1656. Dennehy, T.J., G.C. Unnithan, V. Harpold, Y. Carrière, B. Tabashnik, L. Antilla and M. Whitlow. Susceptibility of Southwestern Pink Bollworm to Bt toxins Cry1Ac and Cry2Ab2 in 2005. 2007 Arizona Cotton Report. Available at http://cals.arizona.edu/pubs/crops/az1437/az14373a.pdf Dennehy, T.J., B.A. DeGain, V. Harpold and R.J. Nichols. Biotype Designations and Insecticide Susceptibility of Southwestern Bemisia tabaci. 2007 Arizona Cotton Report. Available at http://cals.arizona.edu/pubs/crops/az1437/az14373b.pdf Ellsworth, P.C., A. Fournier and T.D. Smith. 2007. Based on Ellsworth, P.C. and J.S. Jones. 2000. Arizona Cotton Insect Losses. Publ. No. AZ1183. University of Arizona, College of Agriculture and Life Sciences, Cooperative Extension, Tucson, Arizona. URL: http://cals.arizona.edu/crops/cotton/insects/cil/cil.html Fournier, A., P.C. Ellsworth and V.M. Barkley. Economic Impact of Lygus in Arizona Cotton: A Comparative Approach. 2007 Arizona Cotton Report. Available at http://cals.arizona.edu/pubs/crops/az1437/az14374a.pdf Ellsworth, P.C., V, Barkley, T. Dennehy, B. DeGain, B. Ellingson, S. Naranjo and M. Sims. Assessment of Knack Field Performance Through Precision Field and Laboratory Bioassays in Cotton 2007 Arizona Cotton Report. Available at http://cals.arizona.edu/pubs/crops/az1437/az14374b.pdf Hutchison, W.D., E.C. Burkness, B. Jensen, B.R. Leonard, J. Temple, D.R. Cook, R.A. Weinzierl, R.E. Foster, T.L. Rabaey, and B.R. Flood. 2007. Evidence for decreasing Helicoverpa zea susceptibility to pyrethroid insecticides in the Midwestern U.S. Online. Plant Health Progress. doi:10.1094/PHP-2007-0719-02-RV. http://www.plantmanagementnetwork.org/sub/php/symposium/hzea/decrease/ Hutchison, W. D., and Weinzierl, R. A. 2007. Increasing concerns about corn earworm susceptibility to pyrethroids in the midwestern USA. Online. Plant Health Progress doi:10.1094/PHP-2007-0719-01-PS. Anilkumar, Konasale J. Marianne Pusztai-Carey, and William J. Moar. 2008. Fitness Costs Associated with Cry1Ac-resistant Helicoverpa zea (Lepidoptera: Noctuidae): A Factor Countering Selection for Resistance to Bt Cotton? J. Econ. Entomol. In Press Sivasupramaniam, S.,W. Moar, L. G. Ruschke, J. A. Osborn, C. Jiang, J. Sebaugh, G. R. Brown, Z. W. Shappley, M. E. Oppenhuizen, J. W. Mullins, and J. T. Greenplate. 2008. Toxicity and characterization of cotton expressing Bacillus thuringiensis Cry1Ac and Cry2Ab2 proteins and characterization of cotton expressing one or both for the control of Lepidopteran pests. J. Econ. Entomol: In Press Crespo, A. L. B., T. A. Spencer, E. Nekl, M. Pusztai-Carey, W. J. Moar, and B. D. Siegfried. 2008. Comparison and Validation of Methods to Quantify Cry1Ab Toxinfrom Bacillus thuringiensis for Standardization of Insect Bioassays. Appl. Environ. Microbiol. In Press Anilkumar, K. J., A. Rodrigo-Simón, J. Ferré, M. Pusztai-Carey, S. Sivasupramaniam, and W. J. Moar. 2008. Production and Characterization of Bacillus thuringiensis Cry1Ac-resistant cotton bollworm, Helicoverpa zea (Boddie) Appl. Environ. Microbiol. In Press Tarver, M. R., R. E. Shade, R. H. Shukle, W. J. Moar, W. M. Muir, L. M. Murdock, and B. R. Pittendrigh. 2007. Pyramiding of insecticidal proteins for control of the cowpea bruchid (Callosobruchus maculatus F.). Pest Mgmt. Sci. 63(5):440-6 Keith, B. A., E. Kalinina, and W.E. Dyer. 2007. Global analysis of gene expression in auxinic herbicide-resistant Kochia scoparia. Weed Sci. Soc. Am. Abstr. 47:35. Eissa, H.F., A. Shokry, O.M. Saleh, A.M. Ramadan, A. Bahieldin and W. E. Dyer. 2007. Genomic characterization of stress-related genes from wild barley. Second International Conference of Genetic Engineering and its Applications. 14-16 Nov., 2007, Sharm El-Sheikh, Egypt. Adams, M. L., Holmes, G., McGrath, M. T., and Olaya, G. 2007. Reduced efficacy of QoI and demethylation-inhibitor (DMI) fungicides on powdery mildew of cucurbits in North Carolina. Phytopathology 97:S2. http://www.apsnet.org/meetings/2007/abstracts/a07ma05.htm McGrath, M. T. 2007. Managing cucurbit powdery mildew and fungicide resistance. Acta Hort. (ISHS) 731:211-216. http://www.actahort.org/books/731/731_29.htm McGrath, M. T., and Davey, J. F. 2007. Evaluation of fungicide programs for management of powdery mildew on pumpkin, 2006. Plant Disease Management Reports 1:V060. McGrath, M. T., and J. Davey. 2007. Occurrence of resistance to QoI, DMI, and MBC fungicides in Podosphaera xanthii in 2006 in New York and implication for controlling cucurbit powdery mildew. Resistant Pest Management Newsletter 16(2):10-12.