Brief Summary of Minutes of Annual Meeting

At years end (CY04) W-6 had 72,332 accessions across 358 genera and 2,188 species (2,444 taxa). There were 1,575 new samples received for inventory. Of these 108 came from National Center for Genetic Resources Preservation (NCGRP) and the other 1,467 were new accessions and assigned interim W6 numbers. A large collection (555 accessions) came from Stoney Wright's collection in Iceland/Greenland area, and 85 came from Fred Muehlbauer collection in Georgia. There were 127 accessions from the ICARDA/Russia/Tajikistan collection. There were 9,025 observation data points were entered into GRIN in 2004 on 601 accessions for 48 descriptors on 21 crops. Eight per cent of the data came from evaluations by cooperators, and the other ninety-two per cent was data take by W-6 personnel. Germination data included 5,198 viability records entered on 4,843 inventories. Of these, 2,784 were tested in Pullman, 616 were contracted out at $6.50 per inventory, and 1,123 tests were done at NCGRP. Of these 5,198 (57%) were considered backlog inventories which means they were more than three years old. There were 21,511 inventories weighed which corresponds closely to the number of barcodes put on inventory containers. Once we get barcodes on containers we try to weigh them. Lastly, there were 463 inventories were sent to NCGRP for backup. During CY04 15,501 accessions were distributed in 20,489 packets, where 11,148(54%) packets were sent to scientists in the US, and 9,341 (46%) packets sent to foreign countries. There were 676 orders filled made up of 487 different requestors, 95 were foreign requestors. The most requested germplasm groups were grass bean, chickpea and alfalfa respectively. The Agronomy program continued a study to identify duplicate accessions within our grass germplasm collection. Dactylis glomerata 4Latar3, two populations of Eragrostis trichoides 4Bend3, and four populations of Bromus inermis 4Manchar3 were used in the replicated study. We used AFLP analysis on the DNA extracted from the Bromus populations and results indicated there were no differences between them. We will collect more field data in 2005 and run AFLP analysis on the Eragrostis and the Dactylis, then compare two years of morphological data to molecular data for all populations. The Horticulture Crops Curator participated in two NPGS sponsored germplasm collection missions. The first was for native legume species in southeastern Oregon and southern Idaho resulting in 38 new accessions. The second was to Uzbekistan for wild and cultivated carrot, garlic and other Alliums. 42 new accession of A. longicuspis, sativum, and other Allium species were added to our collection from this trip. In the Phaseolus Program seed has been collected from plants that were successfully regenerated via the embryo rescue and tissue culture program. These included Phaseolus vulgaris, P. coccineus, P. acutifolius and a few wild Phaseolus accessions ( P. polystachios var. sinuatus, P. maculates, P. maculatus subsp. ritensis, an unknown P. spp. obtained in 1985, and a P. spp. (carteri-nom. inval.). None of these accessions had been grown before this time. We returned to Guatemala more of the bean seed from the accessions that we had received in an exchange program. This has been a very successful way to get more seed into the collection. In a project to optimize pollinator density for Allium ampeloprasum increase Hellier and Clement completed the field study, which involved 32 field cages at Central Ferry. The seed has been collected from the plants and is drying for subsequent weights and germination tests. We anticipate that this will be completed by early summer 2005. The data will be analyzed and a journal article will be prepared. Retention of Viable Neotyphodium Endophyte in Tall Fescue Accession Undergoing Seed Regeneration. This is research in support of the W6 regeneration program. This project was initiated in 2004 with the transfer of 66 greenhouse plants of each of two PI lines to separate field plots in Pullman. The endophyte status (infected, non-infected) of each plant was established prior to the transplant operation. The objective is to determine if viable Neotyphodium fungal endophytes (microbial germplasm) are retained in seed of tall fescue accessions undergoing routine seed regeneration at W6. This will be a multi-year project, and may well extend into 2007 since we must isolate tiller sections on agar to record the presence of viable endophyte. This research follows Steve Clements research on the retention of viable endophyte in seed stored under different storage temperatures, and his surveys of Pullman grass germplasm for diverse Neotyphodium strains, the discovery of which has helped in the commercial development of new perennial grass/non-toxic endophyte associations for forage grass markets in New Zealand, Australia, and the U.S. A multi-year project on wild barley/endophytes/insect interactions was completed in 2004 with the writing and submission of the final research paper. As a result, the number of cereal insect pests adversely affected by wild barley endophytes has increased to three species (from Russian wheat aphid to Hessian fly and Rose grass aphid). A cooperative project with Department of Entomology, WSU on Cereal Leaf Beetle/Perennial Grass/Fungal Endophyte Interactions quantified adult feeding and survival on replicate plants of endophyte (E+) and endophyte-free (E-) tall fescue, perennial ryegrass, and wild barley. Initial results demonstrate the ability of cereal leaf beetle to oviposit on both of these important forage grasses and the ability of some larvae to develop on tall fescue. Additional research will be done in 2005 to better characterize the host plant suitability of common and popular perennial grasses to cereal leaf beetle. To determine wild Cicer germplasm for pod borer (Lepidoptera) resistance, a cooperative project with Dr. Hari Sharma, ICRISAT - India was established and funded through AID. Using wild annual chickpea accessions from the W6 collection, Dr. Clement will look for resistance to larval Spodoptera, an occasional pest of cool season food legumes in the Palouse. In cooperative research with WSU, Dept. of Plant Path., relative growth rates and competitive interactions on natural substrate and agar media were determined for Ascochyta rabiei and several other fungi colonizing chickpea debris. Aureobasidium pullulans and Clonostachys rosea (the latter mycoparasitic on A. rabiei) proved capable of completely inhibiting sexual and asexual reproduction of the chickpea pathogen when pre-inoculated to chickpea debris in vitro. Primary fungal colonizers of chickpea debris were determined for two seasons. The first of two small scale field trials was initiated in Dec 2004 by treating chickpea debris with biological control agents and setting it out to over-winter; chickpea seedlings will be used as trap plants to monitor ascospore release in spring 2005. Benomyl, thiophanate methyl and fludioxonil were tested for ability to inhibit Fusarium oxysporum f. sp. cepae, F. proliferatum and Embellisia allii when pathogens were inoculated to soil for greenhouse-grown garlic and the fungicides used as pre-planting dips for planting cloves. Although each fungicide produced positive effects relative to controls in one or more circumstances, only benomyl consistently exerted significant effects at conventional P values Dugan et. al. published first reports of Onion Yellow Dwarf Virus, Leek Yellow Stripe Virus and Garlic Common Latent Virus on garlic in Washington State. In a cooperative agreement between the Bureau of Land Management (BLM) and W-6 a project was initiated to strengthen native plant conservation and utilization. The project "Maintenance, characterization, storage, and distribution of key native germplasm" will be active until 2007 with expected funding of $120,000 per year. Material for improvement in Kentucky Bluegrass seed yield for no-burn residue management systems is being developed. Yield and yield component analysis was completed on 560 plants representing 10 selected accessions and two years data. Selections for plants with high yield and yield components within the 10 accessions were made resulting in a set of 40 selections. This material was established in the field for seed increase for future studies of selection response in terms of yield and turf quality. Data collection of variation within and among the selected accessions was completed and data analyses are now underway. Using alfalfa as a model system, AFLP markers with populations of about 20 appeared to provide the most information content for cost. However, other systems produced markers that resulted in association patterns among accessions that were similar. Data collection and analysis is mostly completed. This work will led to the next step, which is comparing duplicates, then charactering core collections, and finally, entire collections. AFLP marker analysis was completed on collections of Blue wildrye from the Umatilla Forest in eastern Oregon and Washington, and also throughout western North America. Agronomic data showed that the Umatilla germplasm fell into three groups based on principal component analysis. We found those same group patterns also occurred in molecular data. This means that molecular characterization should provide a valid picture of overall Blue wildrye diversity and is much easier to complete. Preliminary analysis suggested that the diversity in a broad set of western North America was present in the Umatilla Forest. This information will be used by the Forest Service to define seed transfer zones for forest revegetation using Blue wildrye. Some data analysis remains and then a manuscript will be written. Mountain Brome is a key native grass for reclamation and revegetation on federal lands. Thirteen-hundred fifty plants were established at two locations for a total of 2,700. These were characterized for 12 descriptors and entered into spreadsheets in preparation for analysis. A subset of this material was also characterized for AFLP and RAPD marker variation. Both univariate and multivariate statistics are being used to gauge collection diversity and test hypotheses as to the extent that genetic variation can be explained by ecological and geographic information. This work is cooperative with the US FS to help guide decisions regarding seed transfer zones and management of Mountain brome for revegetation activities. Safflower has potential for an excellent rotation crop with cereals in semiarid regions. Winter safflower (fall planted) would provide winter cover, allow better control of grass weeds, increase water-use efficiency and seed yield compared to spring-sown safflower. We have identified germplasm with enough winter hardiness to be potentially useful for fall planting in many regions. Winter survival was correlated with the degree of prostate growth in the fall. However, some accessions with fall prostrate habit had much more winter hardiness then others. This shows that unknown physiological and well as morphological factors are important in winter survival. Variation within winter hardy safflower is being exploited though selection. Replicated plots were established at Central Ferry and Pullman for studies of selection response to determine how much improvement in winter hardiness is possible through mass selection. The conservation and utilization of native plant resources in the western United States is becoming increasingly important. Yet genetic information to identify seed collection sites used for restoration and reclamation is generally lacking. An understanding of the geographic and ecological distance that plant material should be transferred from original source populations is critically needed. Studies using molecular (AFLP) and morphological attributes will be conducted to determine the extent and structure of genetic variation of Allium acuminatum, an important forb native to the Great Basin. Genetic resource management strategies based on biological conservation principals will be developed leading to identification of candidate in situ reserves (field sites where key populations are located). Populations maintained in situ would provide conservation of genetic variation representing eco-geographic areas in the Great Basin. In addition, ex situ conservation will be carried out at the USDAs Agricultural Research Service gene bank at Washington State University, Pullman, WA. Gene bank conservation will provide 1) readily available, source-identified genetic resources for research and increase and 2) security back up of in situ sites. Overall, this project will provide information to federal agency policy makers, Plant Material Centers, and commercial collectors/producers to improve genetic quality and production efficiency of this species. Continued improvement and optimization of molecular marker techniques. Completed training for AFLP, Microsatellite, and SNP analysis using the Beckman CEQ8000 capillary electrophoresis system. This system will be used in 2005 for AFLP analysis of Safflower accessions, and should improve and speed up the process of scoring AFLP markers. Modifed RAPD procedures for analysis using the Licor PAGE system for use with Allium species. This is a compromise between the unmanageable large number of markers produced by AFLP on this large genome, and the crowded, unreadable markers on low resolution agarose gels. Continued to reduce bottlenecks for high-throughput marker analysis by incorporating lyophilization of samples and pulverization using glass beads and a paint shaker. This process works very well on dicots but only moderately well with fibrous grass tissue. Completed acquisition of microsatellite markers at 10 loci for a collection of 5 cultivars and 6 wild alfalfas from Kazakhstan. Four of the loci have been scored so far, and the results forwarded to Stephanie Greene. Chloroplast VNTR, RAPD, Microsatellite, and AFLP markers were compared for characterization of three alfalfa synthetic populations. Manuscript in preparation. Molly Welsh and Ted Kisha joined the Phaseomics Group and began planning for the analysis of Phaseoulus coccineus accessions within the collection. They chose P. coccineus because of its use as a bridge species for gene diversity introduction into P vulgaris. In preparation, we tested AFLP markers for efficacy in identifying duplications in the P. vulgaris collection. CSFL curator, Clare Coyne, planned, organized, obtained external funding and conducted replicated field trail in two cooperator locations for QTL mapping of quantitative resistance to Aphanomyces root rot and Fusarium root rot and presented data at four meetings to peers and one to the U.S. grain legume industry. Cooperated on germplasm evaluations of pea and chickpea for Fusarium root rot and Ascochyta blight, respectively resulting in two peer-reviewed manuscripts and four meeting presentations. Planned and conducted analysis to determine seed protein concentration data on 480 accessions of the pea collection and 40 accessions of chickpea core collection and presented results to industry. Planned, organized, obtained external funding and conducted pea BAC library characterization and utilization experiments in collaboration with visiting scientists. Conceived, planned evaluation experiments, and mentored student-applicant awarded graduate research fellowship to study perennial Cicer (funded). Conceived, planned, submitted and was awarded funding for a USDA-ARS Postdoctoral Fellowship project on haplotyping for association mapping studies in the cool season food legume collections.

Peer Reviewed: Bragg, D. & K. Tetrick. 2004. Insect control in Fall Seeded Canola. Arthropod Management Tests Vol. 30. journal article (accepted - in press) Bragg, D., C. Donohue, & K. Tetrick. 2004. Insect Control in Spring Wheat. Arthropod Management Tests Vol. 30. journal article (accepted - in press) Bragg, D. & K. Tetrick. 2004. Pyrethroid Control of Insects in Fall Seeded Canola, 2003. Arthropod Management Tests. Vol. 30. journal article (accepted - in press) Bragg, D., C. Donohue, & K. Tetrick. 2004. Russian Wheat Aphid Control in Spring Wheat. Arthropod Management Tests. Vol. 30 journal article (accepted - in press) Bragg, D., C. Donohue, & K. Tetrick. 2004. Russian Wheat Aphid Control in Spring Barley. Arthropod Management Tests. Vol. 30. journal article (accepted - in press) Chen W., C. J. Coyne, T. L. Peever and F. J. Muehlbauer. 2004. Characterization of chickpea differentials for virulence assay of Ascochyta blight and identification of chickpea accessions resistant to Ascochyta rabiei. Plant Pathology 53: 759-769. Clement, S.L., Elberson, L.R., Youssef, N., Young, F.L. and Evans, M.A. 2004. Cereal aphid and natural enemy populations in cereal production systems in Eastern Washington. J. Kansas Entomol. Soc. 77:165-173. 2004. Clement, S.L., Elberson, L.R., Bosque-Perez, N.A. and Schotzko, D.J. In press. Detrimental and neutral effects of wild barley-Neotyphodium fungal endophyte associations on insect survival. Entomol. Experimentalis et Applicata. Dugan, F., K. Schubert and U. Braun. 2004. Check-list of Cladosporium names. Schlechtendalia 11: 1-103. Johnson, R.C., V.L. Bradley, and M.A. Evans. Effect of seed sampling method during regeneration on genetic population structure and growth of model ryegrass populations. Plant Genetic Resources Newsletter. (Accepted Nov. 4, 2004) Johnson, R.C., V.L. Bradley, and M.A. Evans. 2004. Inflorescence sampling improves effective population size of grasses. Crop Sci 44:1450-1455. Lupien, S.L., B.C. Hellier and F.M. Dugan. 2004. First report of onion rust Puccinia allii on Allium pskemense and A. altaicum. Plant Disease 88: 83. Moon, C.D., Craven, K.D., Leuchtmann, A., Clement, S.L. and Schardl, C.L. 2004. Prevalence of interspecific hybrids amongst asexual fungal endophytes of grasses. Molecular Ecology 13:1455-1467. Pappu, H.R., B.C. Hellier, F.M. Dugan. First Report of Onion yellow dwarf virus, Leek yellow stripe virus, and Garlic common latent virus in Garlic in Washington State. Plant Disease, Feb., 2005. Paulitz, T.C., F. Dugan, W. Chen and N.J. Grünwald. 2004. First report of Pythium irregulare on lentils in the United States. Plant Disease 88: 309. Pilet-Nayel M-L., A. Moussart, M. Roux-Duparque, C.J. Coyne, C. Onfroy, A. Lesne, R. Esnault, C. Boitel, R. McGee, B. Tivoli and A. Baranger. 2004. Resistance to Aphanomyces root rot in pea. Proceedings of 5th European Grain Legume Conference p. 101-102. Poole, G.J., W.J. Johnston, and R.C. Johnson. Regional climatic characterization of Pacific Northwest, USA, green type Poa annua. Int. Turf Res. Soc. J. Vol. 10. (Accepted Dec. 4, 2004) Reed, K.F.M., Clement, S.L., Feely W.F. and Clark. B. 2004. Improving tall fescue (Festuca arundinacea) for cool-season vigour. Australian Journal of Experimental Agriculture 44:873-881. Published online, non-peer reviewed: Coyne C. J., S. Murray, and G. M. Timmerman-Vaughan. 2004. Identification of RGAs from a pea BAC library using BAC pools. In New directions for a diverse planet: Proceedings of the 4th International Crop Science Congress, Fischer et al eds., p.3.7.3. http://www.cropscience.org.au/icsc2004/ Hellier, B, M. Pavelka. Summary of Fertility Characteristics of the USDA Garlic Collection when Grown in Pullman, WA. http://NARC2004.org. 12/2004. Non-peer reviewed: Clement, S. 2004. Grass endophyte peregrinations at the Western Regional Plant Introduction Station. Pp. 6-10. Proc. 37th Grass Breeders Work Planning Conference (R.C. Johnson, ed.). Western Regional Plant Introduction Station, Pullman, Washington. (Proceedings Paper). Clement, S.L., Youssef, N., Bruehl, G.W., Kaiser, W.J., Elberson, L.R. and Bradley, V. 2004. Effects of different storage temperatures on grass seed germination and Neotyphodium survival. 3 pages (#511). Proc. 5th International Symposium on Neotyphodium/Grass Interactions (R. Kallenbach, C. Rosenkrans, Jr., T.R. Lock, eds.). University of Arkansas, Fayetteville, Arkansas. (Proceedings paper). Clement, S.L. and Popay, A.J. 2004. Consequences of grass-endophyte associations on insects. XXII International Congress of Entomology. Brisbane, Australia (Abstract). Clement, S.L., L.R. Elberson, N.A. Rosque-Perez, D. Schotzke. Detrimental and Neutral Effects of Wild Barley-Neotyphodium EndophyteAssociations on Insect Survival. International Neotyphodium Grass Interactions. 2004. Hellier, B. The Garlic Collection of the USDA, ARS National Plant Germplasm System. The Garlic Press. #44, Fall 2004. Johnson, R.C. V. Bradley, C. Foiles. 2004. Regenerating grass germplasm: Choosing the right compromise. p. 19-24. In R.C. Johnson (ed) Thirty-Seventh Grass Breeders Work Planning Conference, Pullman WA. Johnson, R.C., W.J. Johnston, C.T. Golob. Evaluating Genetic Resources for Sustainable Production of poa pratensis. Grass Breeders Work Planning Conference Proceedings. 2004. Manuscripts: Jenderek, M.M. and Y. Zewdie. Within and between family variability for important bulb and plant traits among sexually derived progenies of garlic. HortScience, Submitted 12/3/0; accepted 1/31/05. Zewdie, Y., M.J. Havey, J.P. Prince, and M.M. Jenderek. The first genetic linkage map of garlic (Allium sativum L.). Journal of ASHS. Accepted 12/4/04. Abstracts and posters: Casler, M.D., R.E. Barker, J.H. Cherney, Y.A. Papadopolous, R.C. Johnson, and M. Jenderek. Breeding non-flowering orchardgrass. CSSA 2004 Abstracts p. 4380. Casler, M.D., R.E. Baker, J.H. Cherney, Y.A. Papadopolus, R.C. Johnson, and M.M. Jenderek. Breeding non-flowering orchardgrass. (Poster). 2004 ASA-CSSA-SSSA meeting. Coyne C. J. , C. Watt, S. McManus, D. A. Inglis, N. Grunwald, K. E. McPhee, M. L. Pilet-Nayel. 2004. Preliminary identification of QTL associated with Fusarium root rot resistance in pea. In ASA-CSSA-SSSA Annual Meetings Abstracts [CD-ROM], p. 1474. Dugan, F.M., T.L. Peever and W. Chen. 2004. Fungi resident in chickpea debris and competitive interactions with Ascochyta rabiei. Phytopathology 94: S27. Havey, M.J., M.M. Jenderek, K. Sink, Ch. Foo, and Ch. Town. Expressed sequence tags of onion for comparative mapping of the Asparagales. International Symposium on Edible Alliaceae, Beijing, China. March 2004 (Oral presentation and abstract on line). Hogue, J.A., V.J. Erickson, and R.C. Johnson. AFLP, phenotypic, and site diversity of blue wildrye (Elymus glaucus). CSSA 2004 Abstracts p. 5597. Hogue, J.A., V.J. Erickson, R.C. Johnson, T.J. Kisha, C.L. Foiles, and V.L. Bradley. 2004. AFLP, phenotypic, and site diversity of Blue Wildrye (Elymus glaucus). Agronomy Abstracts (5597). Jenderek, M.M. Cladode and flowering characteristics of the USDA Opuntia sp. germplasm collection. (Poster) AAIC, New Uses Council, Joint Meeting, Industrial Crops and Uses to Diversify Agriculture, 9/12-22/04. Program and Abstracts:46. Jenderek, M.M. Evaluation of diversity in seed production of the USDA Limnanthes alba germplasm collection. (Poster). 2004 ASA-CSSA-SSSA Annual Meeting, Abstracts:326. Jenderek, M.M., F. Dugan and R.M. Hannan. Tolerance to rust (Puccinia allii) in seed derived garlic progenies. (Poster) 2004 ASHS, Program and Abstracts:775. Johnson, R.C., W.J. Johnston, C.T. Golob, F.B. Bertoli, M.C. Nelson, T.J. Kisha. Evaluation and Enhancement of Kentucky Bluegrass Genetic Resources for Sustainable Production. ASA-CSSA-SSSA Annual Meeting Abstracts. 2003. p. 916495. Johnson, R.C., Li Dajue, C.L. Foiles. Variation in winter hardiness among safflower accessions. CSSA 2004Abstracts p. 4647. Kisha, T.J. 2004. Comparing molecular marker types for cost and information content. In: Effective use of molecular markers in genetic conservation and improvement of horticultural crops. Sponsored by: Genetics and Germplasm Working Group (GG). American Society for Horticultural Science Abstracts. (Invited Speaker) July 17-20 Kisha, T.J., S.L. Clement, L.R. Elberson. Comparison of PCR and Atem Isolation Based Methods for Fungal Endophyte Detection in Tall Fescue. Agronomy Abstracts. 2003. Kisha, T.J., R.C. Johnson, D.Z. Skinner, G.R. Bauchan, and S.L. Greene. Variance of molecular distances among alfalfa synthetic populations by marker type. CSSA 2004 Abstracts p. 5781. Kisha, T.J., R.C. Johnson, D.Z. Skinner, G.R. Bauchan, and A.L. Greene. 2004. Variance of molecular genetic distances among alfalfa synthetic populations by marker type. Agronomy Abstracts (5781). Pentecost G., S. L. Greene, B. Hellier, Coyne C.J. and A. Raven. 2004. Using Dynamic Modeling to Locate Native Legumes. In ASA-CSSA-SSSA Annual Meetings Abstracts [CD-ROM], p. 1294. Watt C., Razai L., and C.J. Coyne. 2004. Molecular diversity of USDA pea core collection. In ASA-CSSA-SSSA Annual Meetings Abstracts [CD-ROM], p. 1092. Zewdie,Y., M.M. Jenderek, M.J. Havey, and J.P.Prince. Development of the first linkage map in garlic. (Poster) 2004 Plant and Animal Genome XII, Final Abstract Guide:257. Zewdie,Y., M.M. Jenderek, M.J. Havey, and J.P.Prince. Genetic linkage map of garlic (Allium sativum). (Poster) 2004 ASHS, Program and Abstracts:775. Book chapter and Proceedings in 2004: Bertoli, F.B., R.C. Johnson, B. Lucaroni, M. Romani, L. Russi, E. Piano, and M. Falcinelli. 2003. Agronomic and molecular evaluation of Kentucky bluegrass (Poa pratensis L.) Germplasm collected in Italy. Czech J. Genet. Plant Breed. 39 (Special Issue): 330-332. Coyne C.J., S. Murray and G.M. Timmerman-Vaughan. 2004. Utilization of a 3X HindIII pea BAC library to identify clones with low copy number genes. Proceedings of 5th European Grain Legume Conference p. 611. Coyne C.J., N. J. Grünwald, D.A. Inglis, K.E. McPhee and M.-L. Pilet-Nayel. 2004. Inheritance of Fusarium root rot resistance in pea using RILs. Proceedings of 5th European Grain Legume Conference p. 340. Dugan, F.M. 2004. Glossary, pp. 637-672 in: Biodiversity of Fungi: Inventory and Monitoring Methods, ed. by Mueller, G. M., G. F. Bills, and M. S. Foster. Elsevier Academic Press, Amsterdam. Dugan, F.M., and S.L. Lupien. 2004. Quiescent and endophytic fungi in grass family hosts: mutualists, pathogens, saprophytes and hitchhikers, pp. 11-15 in: R.C. Johnson (ed.), Proceedings of the 37th Grass Breeders Work Planning Conference, May 14-17, 2002, Moscow, Idaho. Johnson, R.C. 2004. Proceedings of the Thirty-seventh Grass Breeders Work Planning Conference. Pullman WA. 91p. (Editor) Johnson, R.C., W.J. Johnston, and Charles Golob. 2004. Characterization and Enhancement of the USDA-ARS Kentucky Bluegrass Collection. p. 45-52. In R.C. Johnson (ed) Thirty-Seventh Grass Breeders Work Planning Conference, Pullman WA. McPhee K.E. , D.A. Inglis and C.J. Coyne. 2004. Linkage map location of Fusarium wilt race 2 (Fwn) in pea. Proceedings of 5th European Grain Legume Conference p. 342. Peer-reviewed, submitted: Mimura M, C.J. Coyne and T.A. Lumpkin. 200X. Genetic Diversity of Edamame Soybean, [Glycine max (L.) Merr.] from Japan, China and the U.S. Genetic Resources and Crop Evolution (submitted Dec. 15, 2004). Infantino, A., M. Kharrat, L. Riccioni, C.J. Coyne, K.E. McPhee, N.J. Grünwald. 200X. Screening techniques and sources of resistance to root diseases in legumes. Euphytica (submitted Dec. 17, 2004). Muehlbauer F.J. , S. Cho, A. Sarker, CJ. Coyne, P.N. Rajesh and R. Ford. 200X. Application of biotechnology in breeding lentil for resistance to biotic and abiotic stress. Euphytica (submitted Dec. 31, 2004). Baranger A., Higgins, Bleve-Zacheo, M-L Pilet-Nayel, C.J. Coyne, G.M. Timmerman, T. Warkentin, Taylor, A. Maule, Roman, Torres. 200X. Dry pea breeding for resistance against major biotic stresses: from classical to MAS breeding. Euphytica (submitted Nov. 30, 2004). Pilet-Nayel M.L., F.J. Muehlbauer, J.M. Kraft, R.J. McGee, A. Baranger and C.J. Coyne. 200X. Consistent QTLs in pea for partial resistance to Aphanomyces euteiches isolates from the United States and France. Phytopathology (submitted October 15, 2004). Publications: Description / citation: Bragg, D., C. Donohue, & L. Knettle. 2004. Insect Control in Spring Dry Peas. Arthropod Management Tests. Vol. 30. Greene, S.L., T. Minoura, J.S. Steiner, 2004. Germplasm collection exploration and mapping software: something new for the NPGS Medicago and Trifolium collections. Joint Conference of the 39th North American Alfalfa Improvement Conference and the 18th Trifolium Conference, July 18-21, Quebec City, Canada. Greene, S.L. 2004. Medicago truncatula collection characterization. Invited presentation in the Workshop on New Directions for Forage Legumes, Joint Conference of the 39th North American Alfalfa Improvement Conference and the 18th Trifolium Conference, July 18, Quebec City, Canada. Greene, S.L 2004. Morphological evaluation of the USDA NPGS Medicago truncatula collection. Joint Conference of the 39th North American Alfalfa Improvement Conference and the 18th Trifolium Conference, July 18-21, Quebec City, Canada. Greene, S.L., M. Gritsenko, G. Vandemark. 2004. Relating morphologic and RAPD marker variation to collection site environment in wild populations of red clover. Genetic Resources and Crop Evolution 51:643-653. Mosjidis, J.A. S.L. Greene, K. A. Klingler, A. Afonin. 2004. Isozyme Diversity in Wild Red Clover Populations from the Caucasus. Crop Science 44:665-670. Pentacost C.G., S.L. Greene, B. Hellier, C.J. Coyne, A. Raven. 2004. Automated spatial data collection: a critical step in dynamic modeling. ASA Annual Meetings, Nov 3, Seattle, Washington.