Brief Summary of Minutes of Annual Meeting

NRSP-6 TAC 2007 MINUTES Peninsular Agricultural Research Station (PARS) U.S. Potato Genebank (USPG) Sturgeon Bay, WI June 21, 2007 Creighton Miller, Chair, NRSP-6 TAC 07. Chuck Brown, Vice Chair, NRSP-6 TAC 07 (absent) David Douches, Secretary, NRSP-6 TAC 07 (absent); Isabel Vales acted as Secretary. The meeting was called to order at 8:20 a.m. Attendance Attendance was poor this year due to budget constrains, travel cancellations and/or conflicts with other responsibilities. Present: Ed Ashworth, John Bamberg, C. Y. Hu, Molly Jahn, Max Martin, Creighton Miller, Jiwan Palta, Phil Simon, Richard Tarn, Christian Thill, Gail Wisler, Isabel Vales Absent: Chuck Brown, David Douches, Shelley Jansky, David Spooner, CSREES and APHIS not represented. Morning Session Preliminaries: 1. Welcome, introductions, announcements, distribution of documents 2. The 2007 agenda was distributed. No additions were made and the agenda was approved. 3. The 2006 minutes were distributed. Only a small modification was made: Learned that Hu is both Lead Administrative Advisor to NRSP-6 and Western Region Administrative Advisor. The minutes were approved as distributed. Isabel Vales moved acceptance and Creighton Miller seconded. 4. A Resolutions Committee was appointed by Miller, with by C.Y. Hu as Chair. Pressing Issues: - Interaction with CIP o Getting materials was resolved o APHIS/Quarantine problems have been resolved - Personnel issues  quick overview - Budgets. Where are we? What are the future plans? Tour: A tour of the USPG and PARS facilities (laboratories, greenhouse and field) started at 8:35 a.m. A handout with the names of staff members was distributed: - Bamberg, ARS Lead Scientist and NRSP-6 Project Leader - Martin, UW Academic Staff and NRSP-6 Project Assistant - Stoneman, ARS Technician - Schartner, ARS Technician - Fernandez, UW Classified - Del Rio, UW Academic Staff - Temporary Workers and Students - Associated Professional Staff: - David Spooner: ARS Taxonomist, Collector and Herbarium Manager - Shelley Jansky: ARS, germplasm evaluator and enhancer - Phil Simon: ARS Research Leader - Jiwan Palta: Formal Cooperative Agreement, research & U.WI Breeder Afternoon Session (Reports and Comments) Coordination of Genebanks/Intergene Bank - John Bamberg " In 1970s, CIP suggested cooperation. " In the 1990s, linkages between banks were established, and there was a coordination of holdings. There has been little activity during the past few years. " In 2005 there was a resurrection of global diversity. " Collaborations continue with the CIP and German/Dutch potato collections. Some projects involve in situ work  collections and surveys are important. " A cooperative agreement with CIP to have student exchanges would be good, and this is in the works. " Important aspects related to coordination of Genebanks are to avoid duplication and to foster interaction and information sharing. 5/6. Report from Lead Administrative Advisor to NRSP-6  C.Y. Hu " Hu will host the Western Regional meeting in Hawaii in March. " Four centers hold germplasm in addition to NRSP-5 and NRSP-6 - Geneva, NY - Experiment, GA - Ames, IA - Pullman, WA " Funding for the four above mentioned centers is regional. " NRSP-6 funding comes from the federal level. NRSP-6 funding is evaluated by the National Germplasm Review Committee. The recommendations are to restore funds at the 05-06 level ($150,000). Funding for NRSP6 is on the agenda for the July regional meeting. Funding depends on voting which will take place in September. Ching Yuan Hu, Ph.D. (7/20/07)  written report: The NRSP Review Committee met on June 6, 2007 in Kansas City to discuss NRSP budget proposals. Committee members present were Lee Sommers (CO), Chair and W rep; Marshall Martin (IN), NC rep; Bill Vinson (WV), NE rep; Craig Nessler (VA), S rep; Al Parks (Prairie View A&M), ARD rep; Larry Miller, CSREES rep; Eric Young, S Executive Director; Mike Harrington, W Executive Director; and , Don Latham (IA), Stakeholder rep. During the Western Directors Joint Summer Meeting (July 16-18), Lee Sommers made a presentation on NRSP budget recommendations which the committee will be submitting to the Experiment Station Section at its September annual meeting in Philadelphia. The following is an excerpt from Lees report on NRSP6: NRSP-6. Inter-Regional Potato Introduction Project. The amount requested for FY08 was $110,000. This project is an essential component of the National Plant Germplasm System, and the funding request is consistent with maintaining ongoing support from the SAES for the project. Motion by Nessler to accept budget request. Second by Latham. Motion passed. I anticipate the NRSP Review Committee recommendations will be approved by the directors attending the ESS meeting. I spoke to Lee about NRSP6 budget request this morning. He indicated it is too late to make a request to change our budget proposal; but suggested us to submit a budget next year at the level of 2006 funding ($150K). I will work with John to prepare FY09 NRSP-6 budget proposal in January. Issues to discuss: recover some costs (for example shipping and handling). Probably not necessary. Recipients pay for shipping if a fast delivery is requested. Shipping and handling costs will be difficult to manage through the USDA-ARS system. Molly Jahn, Dean of the WI College of Agriculture, should be the Lead Administrative Advisor for this group, but since she is new to the group, Hu agreed to remain as the Lead AA for two more years. 11. Budget Report and Outlook - Bamberg " From the Directors office: USDA does not have a budget for 2007. " The presidents budget does not include special grants. Special grants CSREES disappeared, which was a major setback for participating states. $160 mill. moved into formula funds (Hatch funds) " Now working on a new appropriation bill, farm bill " Congress wants to move formula funds into multi-state competitive research. " New initiative: CREATE 21 (Creative Research and Teaching) " Special grants will not go away, but the Chair of the House Committee can reduce them. For next year, theyll reduce them to half. This will be discussed in September. 9. Additional Genebank Staff and Resources Report John Bamberg and Jiwan Palta " Funding looks good. It will probably go back to the $150,000 level. " The Dept. of Horticulture will reduce FTE by 1/3. The general feeling was that the interactions and synergy between USDA-ARS researchers and the university has been excellent, and it will be sad to see it decline due to budgetary issues. " Some money was received at the end of the year and will continue at the same level. " Annual report can be downloaded from the Web. Introductions  stocks from the SW B classification  Spooner was appointed to head collection and classification Preservation  Virus PSTV Part B. Evaluation International collaborations Nutrition Tools  Germplasm, mutants Alfonso del Rio  diversity impact E. Distribution of materials: normal year F. Visitors from China, from media Useful findings  There are lots of potato varieties with exotic germplasm Section 5. Continue Section 6. Publications. Note: publications should be requested from collaborations/germplasm recipients Set up parameters, metrics will be useful to reflect damage if funding is disrupted. Formal Cooperative Agreements - Jiwan Palta " Cooperative studies resulted in 2 NRI grants funded and training of several students. " Research Topics: Cold tolerance and acclimation from biochemical and genetic perspectives " Cooperative agreement under way to study GA and tuberization, tuber acidity, P, genetics of tuber calcium and carbosypeptidase acidity. " Cooperation with CIP  trips 10. Collections and Taxonomy  John Bamberg " CIP trips  able to collect again " There are prospects about collecting in Peru. " Engaging and showing benefit sharing are important 12/13. USDA, ARS Potato NPL  Gail Wisler " Grants from USDA, ARS, and NPC resolved. " The idea of expanding membership to include more breeders and the private sector was positively received. " Potato cyst nematode discussion 8. Agriculture/Agrifood Canada. - Richard Tarn " Richard is the PI for the potato breeding program. " Participated in Canada Plant Resources Project. 132 accessions: 54 Canadian bred varieties. Working closely with seeds of diversity. They identified heirloom varieties of importance. " Standard clones used in pathology as disease resistance checks " Breeding lines of importance " An annual newsletter is prepared. Check the web page Potato Research Center GRINCA: Canadian version of GRIN 45 requests (511 units) Most materials are in vitro Personal information should not be disclosed Official minutes should contain a synthesis of the reports Technical reports are for internal communication and follow mission aspects 7. Regional and ARS Tech. Rep. Reports (posted on Genebank web site) Western Region: Isabel Vales Southern Region: Creighton Miller North Central Region: Christian Thill National Program Staff Report: Gail Wisler Canada: Richard Tarn Statistics Report status of orders/research Needs/advise 16. Review and Approve Resolutions. Chris Thill moved to approve and Tarn seconded. Motion passed. 17. Elect New Officers and Set next Meeting date Chuck Brown, Vice Chair to Chair in 2008 David Douches, Secretary to Vice Chair in 2008 Isabel Vales, Acting Secretary to Secretary in 2008 Christian Thill moved to approve and Palta seconded. Motion passed. 2008 location: Cornell. Thill moved acceptance of meeting location and Gail seconded. Motion passed. The meeting was adjourned at 6:02 p.m. Resolutions: Resolution #1. WHEREAS, Dr. Steven Slack served as North Central Region Administrative Advisor and the Lead Administrative Advisor to the NRSP-6 Potato Germplasm Project; and WHEREAS, he conscientiously provided liaison between the project and the USDA/NC Regional Association, as well as Experiment Station Section; therefore, be it RESOLVED, that NRSP-6 TAC expresses highest appreciation for Dr. Slacks leadership and many contributions to the NRSP-6 and the development and use of potato species, and be it further RESOLVED, that an original of this resolution be provided to Dr. Steven Slack, and that a copy be filed as part of the official minutes of this meeting. Resolution #2. WHEREAS, Dr. Steve Reiling served as Northeast Region Administrative Advisor to the NRSP-6 Potato Germplasm Project; and WHEREAS, he conscientiously provided liaison between the project and the USDA/NE Regional Directors Association, be it RESOLVED, that NRSP-6 TAC expresses highest appreciation for Dr. Reilings leadership and many contributions to the NRSP-6 and the development and use of potato species, and be it further RESOLVED, that an original of this resolution be provided to Dr. Steve Reiling, and that a copy be filed as part of the official minutes of this meeting. Resolution #3. WHEREAS, the NRSP-6 TAC met at the Peninsular Agricultural Research Station, Sturgeon By, WI, June 21-22, 2007; and WHEREAS, those attending were educated and stimulated by meetings and the station tour; and WHEREAS, the location for the meeting was outstanding and the accommodations were both compatible and conducive to effective interaction resulting in a successful meeting; therefore, be it RESOLVED, that the NRSP-6 TAC expresses its appreciation to Dr. John Bamberg and Mr. Max Martin for arranging the facilities and coordinating the meeting and the station tour, and be it further RESOLVED, that an original of this resolution be provided to Dr. John Bamberg and that a copy be filed as part of the official minutes of this meeting.

A. Introduction of New Stocks A total of 41 accessions were assigned PI numbers in 2006: one clone from Korea, one late blight clone from Mexico, one mapping population parent from CIP, 25 tuber moth resistant clones from CIP, and 13 accessions collected from Arizona. These accessions are now available from the NRSP-6 Solanum germplasm collection. The NRSP-6 web page (http://www.ars-grin.gov/nr6) has been updated to include all new stocks and screening information. Clientele that have ordered from NRSP-6 in the past four years are contacted three times per year informing them of new materials that are now available either as true seed, tubers, in vitro plantlets, or herbarium samples. A new service we are offering is dried ground leaf samples for DNA. For foreign requesters this is useful since there are no quarantine restrictions on dried material. B. Classification Dr. Spooner et al. have published and are working on five different areas of potato research: 1) molecular markers for genebank studies, 2) cultivated potato origins, 3) relationships in wild tomatoes and potatoes, 4) the predictive power of taxonomy relative to disease resistance data, and 5) linkage map of late blight in wild potatoes. C. Preservation and Increase of Stocks In 2006, a total of 223 accessions were increased as botanical seed populations. A total of 600 potato spindle tuber viroid (PSTVd) tests were performed on seed increase parents, seedlots and research materials. Germination tests were performed on 1,307 accessions, ploidy determinations were made on 40 accessions, and tetrazolium seed viability tests were done on 31 seedlots. D. Evaluation International collaboration. 1) with CIP, study of the impact of agrichemicals on in situ wild potato reproduction continued, and we initiated a related project to assess the impact of mining pollution on wild potato reproduction. The frost resistant breeding family developed by NRSP-6 staff was evaluated in Peru, and results appear to be promising. The elite clonal selections from this population grown in Hancock, Wisconsin had good tuber type and cold hardiness to 5oC. 2) with CIP, calcium application trials in the highlands showed impressive yield improvement, and suggest our parallel ongoing program to enhance calcium uptake efficiency via S. microdontum introgression might have impressive application in some locations in the Andes. 3) with CIP, progress was made on a joint project to study in situ germplasm capture (see below) and molecular-based assessment of allele density. 4) with PICTICPAPA: The entire collection of S. microdontum was evaluated for late blight in Toluca, Mexico (results pending). Nutritional. 1) Potato potassium is in a unique position to mitigate hypertension, which has huge health and economic impact. Potassium levels in the tubers are also correlated to the incidence of black spot bruise. We screened and found significant species differences in K uptake potential (funding anticipated from CGC). 2) Work continued with Dr. C. Millers lab screening for antioxidants in uncolored wild potato species tubers. We found an important GxE effect, demonstrating that some germplasm is only high antiox when grown artificially in the greenhouse, not in the field (two papers submitted). 3) Plans were made to preselect and screen the primitive cultivated species collection for high phenolics. 4) We began researching and planning experiments to examine anticancer potential of high tuber tomatine. 5) We characterized exotics and adapted materials for Potato Carboxypeptidase Inhibitor, a tiny heat- and digestion-resistant protein reported to inhibit some of the most pernicious human cancers (funded by the Crop Germplasm Committee, CGC). We staged parents for making and testing segregating hybrids for all of these traits. Mutants and other new traits. 1) We initiated crossing for adapted tubers in the background of the crazy sepal sterile floral mutant. BSA of crazy and flowering plants are planned for a fair test of the potential yield improvement without the sink in flowers. 2) Continued tests to relate tuber pH to other traits like calcium and antiox. S. microndontum was newly identified as a species for which some populations tubers are extremely acidic (10x most cultivated types). 3) Produced calcium introgression hybrids at 2x and 4x level and evaluated in WI and Hawaii. 4) Confirm that other GA mutants published as pito and ga2 are both likely the same as our ga1. Staged for study of spontaneous reversion to the functional allele. 5) Purple-less fendleri: Eliminated genome suppression as explanation for distorted segregation observed in some mutant testcrosses. Genebank technology and diversity management. 1) Found that long-term germination is declining more rapidly outside of local -20 and NSSL storage, so moved our active inventory to -20. 2) Found germination is not biased by density of sowing. 3) Found seedlots generated in 1986 with and without extra fertilization do not show clear germination effect due to seed quality after 20-yr storage. 4) Found seedlings are taller under sodium vs metal halide lamps. 5) Characterized 100 microdontum pops for pH, calcium, antiox, cs1 mutant and late blight pursuant to testing effectiveness of DNA-based core collection within a species. 6) Tested seed increase phenology and found later summer plantings produce more seeds per pollination. 7) With CIP cooperators continued case study of capture of diversity at easy-vs-hard collecting sites. 8) Found proximity of other species does not explain distinction of northern S. verrucosum populations in Mexico. 9) Published results showing small DNA differences between reputed duplicates in NRSP-6 and CIP collections. 10) Published conclusion that no loss of diversity happens at selection of excess small seedlings at transplant for inbred species. E. Distribution NRSP-6 distributions are shown in the table below. Stocks were sent to clientele in 25 states of the USA and 11 other countries. VOLUME AND TYPES OF STOCKS DISTRIBUTED Units1 Category S TF TS IVS DNA PL HERB TOT PIs Domestic 3,794 214 1,158 617 0 11 0 5,794 3,088 Foreign 481 0 16 20 42 0 0 559 529 NRSP-62 9,192 0 62 284 0 0 0 9,538 2,271 Total 13,467 214 1,236 921 42 11 0 15,891 5,888 1 Types of stocks sent/(number of seeds, tubers or plantlets per standard shipping unit): S= True Seeds/(50), TF= Tuber Families/(10), TS=Tuber Stocks/(3), IVS=In Vitro Stocks/(1), DNA=DNA samples(1), PL=Plants in plugs/(1), Her= Herbarium/(1). 2 Includes chromosome counts, germination tests, ID and Taxonomic check plantings, in vitro maintenance, seed increases, PSTV tests, miscellaneous plantings, and NSSL seed backup. F. Visitors From Other Countries Dr. Shaoguan Duan-China; Dr. Liping Jin-China; Dr. Shuzhi Jaing-China; Dr. Kaiyun Xie-China; Dr. Wenbo Pan-China; Dr. Conghua Xie-China; Dr. Jun Liu-China; Dr. Nune Sarukhanyan-Armenia; Dr. Klaus J. Dehmer-Germany; Dr. Glenn Bryan-Scotland; Dr. Gavin Ramsay-Scotland; Dr. Caroline Marques Castro-Brazil

Bamberg, John. 2006. Crazy Sepal: A new floral sepallata-like mutant in the wild potato Solanum microdontum Bitter. Am J Potato Res 83:433-435. Bamberg, J.B. and A. del Rio. 2006. Seedling transplant selection does not cause genetic shifts in genebank populations of inbred potato species. Crop Sci 46:424-427. Bamberg, John, Charles Fernandez and Alfonso del Rio. 2006. A new wild potato mutant in Solanum stoloniferum Schltdl. lacking purple pigment. Am J Potato Res 83:437-445. Bamberg, J., J.P. Palta and M. Martin. 2006. Using a wild species, S. microdontum, to move high calcium trait to the cultivated potatoes. In Potato Association of America/Solanaceae 2006 Annual Meeting. p. 163 (Abstract) Busse, J.S., J. Bamberg and J.P. Palta. 2006. Correlation between aerial and tuber calcium accumulation in Solanum genotypes segregating for tuber calcium uptake efficiency. In Potato Association of America/Solanaceae 2006 Annual Meeting. p. 204 (Abstract) Del Rio, A.H., J.B. Bamberg and Z. Huaman. 2006. Genetic equivalence of putative duplicate germplasm collections held at CIP and US Potato Genebanks. Am J Potato Res 83:279-285. Del Rio, Alfonso, John B. Bamberg and Charles Fernandez. 2006. Assessment of the genetic structure of in situ populations of wild potato Solanum fendleri eco-geographically dispersed in the Chiricahua mountains, Arizona. Am J Potato Res 83:108. (Abstract) Ghislain, M., D. Andrade, F. Rodriguez, R.J. Hijmans, and D.M. Spooner. 2006. Genetic analysis of the cultivated potato Solanum tuberosum L. Phureja Group using RAPDs and nuclear SSRs. Theor Appl Genet 113:1515-1527. Jansky, S.H., R. Simon and D.M. Spooner. 2006. Testing taxonomic predictivity. Crop Sci 46:2561-2570. Kuang, H.H., F.S. Wei, M.R. Marano, U. Wirtz, X.X. Wang, J. Liu, W.P. Shum, J. Zaborsky, L.J. Tallon, W. Rensink, S. Lobst, P.F. Zhang, C.E. Tornqvist, A. Tek, J. Bamberg, J. Helgeson, W. Fry, F. You, M.C. Luo, J.M. Jiang, C.R. Buell, and B. Baker. 2005. The R1 resistance gene cluster contains three groups of independently evolving, type I R1 homologues and shows substantial structural variation among haplotypes of Solanum demissum. Plant J 44(1):37-51. Lozoya-Saldana, H., O. Barrios and John Bamberg. 2006. Phytophthora infestans: races vs genotypes in the Toluca Valley, Mexico. Am J Potato Res 83:122. (Abstract) Nzaramba, Ndambe M., John Bamberg, Douglas C. Scheuring, and J. Creighton Miller Jr. 2006. Antioxidant activity in Solanum species as influenced by seed type and growing location. Am J Potato Res 83:127. (Abstract) Peralta, I.E., S. Knapp and D.M. Spooner. 2006. Nomenclature for wild and cultivated tomatoes. Rep Tomato Genet Coop 56:6-12. Spooner, D.M. and A. Salas. 2006. Structure, biosystematics, and genetic resources. In: J. Gopal and S.M. Paul Khurana (eds.) Handbook of potato production, improvement, and post-harvest management. Haworths Press, Inc., Binghampton, New York. Pgs.1-39. Vega, S.E., M. Aziz, J. Bamberg, A. Verma, and J.P. Palta. 2006. Screening potato germplasm for carboxy-peptidase inhibitor and its potential anticancer property. In Potato Association of America/Solanaceae 2006 Annual Meeting. p. 160 (Abstract) Vega, S.E., J.P. Palta and J. Bamberg. 2006. Exploiting cultivated germplasm to breed for enhanced tuber quality. In A.J. Bussan and M. Drilias (eds.). Proceedings of the Wisconsins Annual Potato Meeting. pp. 143-144. (Abstract) Vega, Sandra E., John B. Bamberg and Jiwan P. Palta. 2006. Gibberellin-deficient dwarfs in potato vary in exogenous GA3 response when the ga1 allele is in different genetic backgrounds. Am J Potato Res 83:357-363. Vega, Sandra E., Jiwan P. Palta and John B. Bamberg. 2006. Root zone calcium can modulate GA induced tuberization signal. Am J Potato Res 83:135. (Abstract) Vega, Sandra E., Jiwan P. Palta and John B. Bamberg. 2006. Exploiting cultivated germplasm to breed for enhanced tuber calcium accumulation ability. Am J Potato Res 83:136. (Abstract)