SAES-422 Multistate Research Activity Accomplishments Report

Status: Approved

Basic Information

Participants

Roy Kirkwood [kirkwood@cvm.msu.edu] Michigan; John Parrish [parrish@ansci.wisc.edu] Wisconsin; Matt Wilson [Wilson@mail.wvu.edu] West Virginia; Lloyd L Anderson [llanders@iastate.edu] Iowa; Timothy J. Safranski [SafranskiT@missouri.edu] Missouri; Joe Ford [ford@email.marc.usda.gov] USDA-MARC; David Miller [djmille@uiuc.edu] Illinois; Duane Davis [ddavis@oznet.ksu.edu] Kansas; Mark Diekman [mdiekman@purdue.edu] Indiana; Brett White [bwhit1@unlnotes.unl.edu] Nebraska; John Baker [baker@anr.msu.edu] Michigan;

The annual meeting of the NCERA-57 was called to order by John Baker, Committee Advisor, at 8:26 AM on Wednesday May 30. John introduced Jeff Armstrong, Dean of the College of Agriculture and Natural Resources. Jeff welcomed us to campus and invited a discussion about the college and USDA/HATCH issues. In particular, USDA would not have new funds but would redistribute them differently in the future. There was also discussion about how to deal with animal facilities long-term at a College of Agriculture. This included the impact of AAALAC accreditation on operation of animal facilities. Finally Jeff indicated the college was doing well with many new hires. Jeff then introduced Karen Plaut, head of Animal Sciences. She explained the Animal Science Department structure and how it worked. In particular, the reproduction area focuses on dairy but many model species are utilized. There are approximately 300 undergraduates and 100, 2 year, Ag-Tech students with approximately 40 staff. There is an increasing emphasis on transitioning classes to include companion animals as seen in other Animal Science departments across the country. John Baker next went over NCERA-57. Currently, Roy Kirkwood is president, John Parrish is vice-president and Dave Guthrie is the secretary. Minutes of the meeting will be taken by John Parrish and sent to Dave Guthrie who could not attend this year. The renewal process was then reviewed. The rewrite of the committee will need to be done by assigning a writing committee to prepare draft and issues for the 2008 meeting. The committee should be assigned in Jan 2008 with a report at the May 2008 meeting. The rewrite should focus on the success of the symposiums, NRI proposal. It was also mentioned that everyone should look at the NIMMS site and our own website for information on the committee. John Parrish also asked each member to submit some impact statements for use in the NIMMS report. Deb Hamernik, the CSREES representative could not attend and her report was emailed to all committee members. Roy Kirkwood next addressed the meeting logistics. Duane Davis went over the NRI Integrated Project Proposal that was submitted by he and Dave Guthrie entitledSeasonal Infertility in Swine: Research and educational goals to enhance the profitability and sustainability of the swine industry discussing the criticisms of the reviewers. It was decided that some of the members would agree to test of the teaching material at their university and participate in the rewrite. Duane will be heading up the teaching section and we hoped that Dave Guthrie would again head the research section. The station reports then began and continued until 3:15 PM when we traveled to the Diagnostic Center Population and Animal Health building for a tour. We were welcomed at the building by Steve Bolin who gave us a most interesting tour. Great discussion of many topics occurred at dinner. The business meeting was called to order on Thursday May 31 by John Baker who found the gavel and used it to open the business meeting. The committee welcomed Matt Wilson as the representative from West Virginia. It was then mentioned the pointer was missing and Mark Diekman will ensure it is present at the meeting next year. The tentative dates for the next meeting are June 25-26, 2008 at Purdue University. On Wednesday morning we would have station reports and then travel to JBS United, Sheridan Indiana, for a symposium. Station reports and the business meeting will then be on Thursday. Tim Safranski, Mark Diekman, Joe Ford and Matt Wilson will serve as the program committee. Although several topics were suggested, the program committee will discuss topics in consultation with JBS United. The late nature of the meeting was due to an early date for SSR and Mark Diekman's previous commitments. Matt Wilson was elected by unanimous vote to be the new secretary (was that a surprise!). John Parrish reminded everyone of the website for the committee and asked if station reports could be distributed on the website with a password protection. It was decided that we would do this so a record of station reports could be kept for future members and be located all in one spot. A password was discussed but will be distributed only to members of the committee. A further discussion of the NRI proposal was then conducted. It was suggested the focus stay the same but with some changes to the teaching section which will be addressed via email discussion among the members. A big thanks was then extended to Roy Kirkwood for hosting the meeting and making all the arrangements, following which we returned to station reports. The meeting was adjourned at 11:30 AM with the passing of the gavel to John Parrish. Thanks to John Parrish for recording the minutes for me. Respectively submitted Dave Guthrie, Secretary, NCERA-57

Accomplishments

1. A CSREES NRI integrated research proposal was submitted in November of 2006 entitled Seasonal Infertility in Swine: Research and Educational Goals to Enhance the Profitability and Sustainability of the Swine Industry. The proposal was not funded in 2007 and the co- principal investigators Dave Guthrie and Duane Davis with the help of other committee members will revise the proposal and re-submit for funding in 2008. 2. John Parrish is commended for making great progress in the development of the website for the committee http://www.ansci.wisc.edu/ /ncera57/index.html 3. Planning was started was started for the 2008 symposium with Tim Safranski, Mark Diekman, Joe Ford and Matt Wilson serving as the program committee. The tentative plan is to travel to JBS United, Sheridan Indiana, for the symposium on Wednesday. Station reports and the business meeting will then be on Thursday. The topics and title of the symposium will be developed by the program committee in consultation with JBS United. 4. Collaborations: Scientists at University of Nebraska, Lincoln and USDA-MARC have established a collaboration to examine the association between polymorphisms within the promoter for the GNRH gene and FSH production in swine. This research could lead to genetic tests for fertility as well as new methods for improvement of sperm production and litter size in swine. Dave Guthrie and Joe Ford will continue a collaboration on the genetics of the genetics of post-thaw sperm survival in swine. During the past four years we have investigated 12 boar sire lines at the USDA Meat Animal Research Center which were shipped to Beltsville for analysis of viability after cryopreservation. Two of these lines have maintained significantly greater post-thaw survival than the others. The MARC group has developed sets genetic markers spanning the entire genome have been genotyped on all sires of the BX population, a large group of sows born in 2004 and 1008 gilts born in 2005. The set of markers includes over 100 microsatellite markers and approximately 200 SNP markers. We will continue this work to link the trait of sperm cryosurvival to microsatellite markers and single nucleotide polymorphisms.

Impacts

  1. Mechanisms of Protein Secretion: Cellular protein transport and secretion is fundamental to the very existence of an organism, regulating important physiological functions such as reproduction, digestion, energy production, growth, neurotransmission, hormone release, water and ion transport, all required for the survival and maintenance of homeostasis within an organism. Only in the last 20 years, the general molecular mechanism of the process has come to light, following discovery of key proteins involved in ER-Golgi transport, and discovery of the porosome - the universal secretion machinery in cells.
  2. In vitro pig embryo production/development: Apoptosis has been demonstrated to occur during preimplantation development both in vivo and in vitro and it is believed to contribute to early embryonic loss. Nuclei of different donor cells support the development to various degrees and confirm previous reports using non-differentiated cells as nuclear donors increase the efficiency of nuclear transfer in the pig. Treatment of oocytes with cycloheximide followed by electroporation is an effective activator of porcine embryonic development. Porcine oocytes can be successfully matured in defined maturation media without gonadotropins, when the media contains fetuin, recombumin and EGF.
  3. Development of the pituitary secreting cells of the young pig: Fluorescence immunocytochemistry revealed specific spatial distribution patterns of somatotrophs, mammotrophs, and mammosomatotrophs. Results of these studies strongly suggest regional specificity of cellular transformation or interconversion to facilitate growth hormone and prolactin secretion as the need for endocrine regulation during the period of rapid growth in the young pig.
  4. Assisted reproductive technology to improve fertility: The committee has demonstrated improvements in the protocols for the use of exogenous gonadotrophins in swine breeding. These improvements allow for the maintenance of fertility of multiparous sows and 10 to 15% increases in farrowing rates of primiparous sows. Using single inseminations of fewer sperm it is possible to reduce the labor costs associated with animal breeding as well as reducing semen costs by 50 to 75%. It was shown that it is possible to mix unfamiliar sows into groups for gestation from 3-weeks after insemination with no detriment sow fertility.
  5. Boar sperm shape associated with semen quality: Analysis of sperm nuclear shape using Fourier Harmonic Analysis was shown to detect changes in sperm shape associated with reduced fertility associated with not only different males but also for individual ejaculates within a male that had reduced fertility Fourier harmonic analysis of sperm nuclear shape was applied to porcine sperm and shown to detect shape changes due to sperm storage at 16°C.

Publications

Almond, G.W., Flowers, W.L. Batista, L. and D'Allaire, S., 2006. Diseases of the reproductive system. In: Diseases of Swine, 9th edition. B. Straw. J. Zimmerman, S. DAllaire, and D. Taylor (eds.) Blackwell Publishing, Ames, IA, pp. 113-148. Wilhelms, K.W., G.A. Kraus, J.D. Schroeder, J.W. Kim, S.A. Cutler, M.A. Rasmussen, L.L. Anderson and C.G. Scanes. 2006. Evaluation of corn-furan fatty acid putative endocrine disruptors on reproductive performance in adult female chickens. Poult. Sci. 85:1795-1797. Wilhelms, K.W., S.A. Cutler, J.A. Proudman, R.V. Carsia, L.L. Anderson and C.G. Scanes. 2006. Lack of effects of atrazine on estrogen-responsive organs and circulating hormone concentrations in sexually immature female Japanese quail (Coturnix coturnix japonica). Chemosphere 65:674-681. Wilhelms, K.W., S.A. Cutler, J.A. Proudman, L.L. Anderson and C.G. Scanes. 2006. Effects of atrazine on sexual maturation in female Japanese quail induced by photostimulation or exogenous gonadotropin. Environ. Toxicol. Chem. 25:233-240. Wilhelms, K.W., K.F. Fitzpatrick, C.G. Scanes and L.L. Anderson. 2006. In ovo exposure to a triazine herbicide: effects on circulating reproductive hormones and gonadal histology in young Japanese quail. Arch. Environ. Contam. Toxicol. 51:117-122. Anderson, L.L. 2006. Invited Minireview titled: Discovery of the porosome- the universal secretory machinery in cells. J. Cell. Mol. Med. 10:126-131. Anderson, L.L. and B.P. Jena. 2006. Fusion pores in growth-hormone-secreting cells of the pituitary gland: an AFM study. In: Force Microscopy: Applications in Biology and Medicine. Edited by B.P. Jena and J.K.H. Hörber. J. Wiley & Sons, Inc., New York, [Chapter 4] pp. 49-67. Wilhelms, K.W., C.G. Scanes and L.L. Anderson. 2006. Lack of estrogenic or anti-estrogenic actions of soy isoflavones in an avian model: the Japanese quail. Poult. Sci. 85:1885-1889. Anderson, L.L. 2006. Editorial Forward for Cell Secretion Review Series  Cell Secretion  finally sees the light. J. Cell. Mol. Med. 10:271-272. Bohan, M.M., L.L. Anderson, A.H. Trenkle and D.C. Beitz. 2006. Effects of dietary macronutrients on appetite-related hormones in blood on body composition of lean and obese rats. Animal Industry Report. A.S. Leaflet R-2081. Bohan, M., L. Anderson, A. Trenkle and D. Beitz. 2007. Nutrient regulation of plasma ghrelin concentration in lean and overweight female humans. Animal Industry Report. A.S. Leaflet R-2214. Lkhagvadorj, S., L. Qu, W. Cai, O. Couture, Y. Wang, L. Anderson, J. Dekkers, D. Nettleson and C. Tuggle. 2007. Use of transcriptional profiling and assessment of blood parameters to understand biological mechanisms controlling feed intake and efficiency in pigs. Animal Industry Report. A.S. Leaflet R-2233. Tinfo, N.S. and C.M. Komar. 2007. A role for peroxisome proliferator-activated receptor in cell survival, not steroid production, in luteal tissue from the rat. Reproduction 133:187-196. Bridges, P.J., C. M. Komar and J.E. Fortune. 2006. Gonadotropin-induced expression of mRNA for cyclooxygenase-2 (COX-2) and secretion of prostaglandins E and F2a in bovine preovulatory follicles is regulated by the progesterone receptor. Endocrinology 147:4713-4722. Bott, R,C,, R.M. McFee,, D.T. Clopton, C.F. Toombs and A.S. Cupp. 2006. Vascular endothelial growth factor (VEGFA) and kinase komain region receptor are involved in both seminiferous cord formation and vascular development during testis morphogenesis in the rat. Biol. Reprod. 75:56-67. Wood, J.R., D.A. Dumesic, D.H. Abbott, and J.F. Strauss III. 2007. Molecular abnormalities in oocytes from women with polycystic ovary syndrome revealed by microarray analysis. J. Clin. Endocrinol. Metab, 92:705-713. Rachakatla RS, Marini F, Weiss ML, Tamura M, Troyer D. 2007. Development of human umbilical cord matrix stem cell-based gene therapy for experimental lung tumors. Cancer Gene Ther. 2007 Jun 29; [Epub ahead of print] Blomberg, L. A., Garrett, W. M., Guillomot, M., Miles, J. R., Sonstegard, T. S., Van Tassell, C. P. and Zuelke, K. A. 2006. Transcriptome profiling of the tubular porcine conceptus identifies the differential regulation of growth and developmentally associated genes. Mol. Reprod. Dev. 73:1491-1502. Guthrie, H.D., Welch, G.R. 2006. Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated flow cytometry. J. Anim. Sci. 84:2089-2100. Guthrie H. D. and Welch G. R. 2007. Use of fluorescence-activated flow cytometry to determine membrane lipid peroxidation during hypothermic liquid storage and freeze-thawing of viable boar sperm loaded with 4, 4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid. J. Anim. Sci. 85:1402-1411. Keefer, C. L., Pant, D., Blomberg, L. and Talbot, N. C. 2007. Challenges and prospects for the establishment of embryonic stem cell lines of domesticated ungulates. Anim. Reprod. Sci. 98:147-168. Rodriguez, K. F., Blomberg, L. A., Zuelke, K. A., Miles, J. R., Alexander, J. E. and Farin, C. E. 2006. Identification of candidate mRNAs associated with gonadotropin-induced maturation of murine cumulus oocyte complexes using serial analysis of gene expression. Physiol. Genomics. 27:318-327. Bracken CJ, Seaman-Bridges JS, Safranski TJ, Lucy MC. 2006. Ovarian follicular development, estrus, and ovulation in seasonally anestrous sows treated seven days post weaning with equine and human gonadotropins. J. Swine Health Prod. 14:207-209. Lovercamp KW, Safranski TJ, Fischer KA, Manandhar G, Sutovsky M, Herring W, Sutovsky P (2006) Arachidonate 15-lipoxygenase and ubiquitin as fertility markers in boars. Theriogenology, 67: 704-718. Mesa H, Safranski TJ, Cammack KM, Weaber RL, Lamberson WR. 2006. Genetic and phenotypic relationships of farrowing and weaning survival to birth and placental weights in pigs. J Anim Sci., 84:32-40. Li, R., L. Lai, D. Wax, Y. Hao, C. Murphy, A. Rieke, M. Samuel, M. Linville, S. Korte, R.W. Evans, J.R. Turk, J.X. Kang, W.T. Witt, Y. Dai, R.S. Prather. 2006. Cloned transgenic swine via in vitro production and cryopreservation. Biology of Reproduction 75:226-230. Hao, Y., N. Mathialagan, E. Walters, J. Mao, L. Lai, D. Becker, W. Li, J. Critser, R.S. Prather. 2006. Osteopontin reduces polyspermy during IVF of porcine oocytes. Biology of Reproduction 75:726-733. Katayama, M, Z. Zhong, L. Lai, P. Sutovsky, R.S. Prather, H. Schatten. 2006. Mitochondria distribution and microtubule organization in fertilized and cloned porcine embryos: implications for developmental potential. Developmental Biology 299:206-220. Liu, Z.H., H. Schatten ,Y. H. Hao, L.X. Lai, D. Wax, M. Samuel, Z.S. Zhong, Q.Y. Sun, R.S. Prather. 2006. The nuclear mitotic apparatus (NuMA) protein is contributed by the donor cell nucleus in cloned porcine embryos. Frontiers in Biosciences 11:1945-1957. Hao, Y., L. Lai, G.-S. Im, Z. Liu, D. Wax, M. Samuel, C.N. Murphy, P. Sutovsky, R.S. Prather. 2006. Developmental competence of porcine parthenogenetic embryos relative to embryonic chromosomal abnormalities. Mol. Reprod. Dev. 73:77-82. Yong, H.Y., Y. Hao, L. Lai, R. Li, C.N. Murphy, A. Rieke, D. Wax, M. Samuel, R.S. Prather. 2006. Production of a transgenic piglet by a new sperm injection technique in which no chemical or physical treatments are used for oocytes or sperm. Mol. Reprod. & Dev. 73:595-599. Im, G.-S., J.-S. Seo, I.-S. Hwang, D.-H. Kim, S.-W. Kim, B.-C. Yang, B.-S. Yang, L. Lai, R.S. Prather. 2006. Development and apoptosis of pre-implantation porcine nuclear transfer embryos activated with different combination of chemicals. Mol. Reprod. Dev. 73:1094-1101. Bjerregaard, B., H.G. Pedersen, A.S. Jakobsen, L.F. Rickords, L. Lai, H.T. Cheong, M. Samuel, R.S. Prather, F. Strejcek, Z. Rasmussen, J. Laurincik, K. Schellander, H. Niemann, P. Maddox-Hyttel, P.D. Thomsen. 2006. Activation of ribosomal RNA genes among cells of in vitro produced and cloned porcine embryos. Mol. Reprod. & Dev. 74:35-41. Agca, C., J.E. Ries, S.J. Kolath, J.-H. Kim, L.J. Forrester, E. Antoniou, K.M. Whitworth, N. Mathialagan, G.K. Springer, R.S. Prather, M.C. Lucy. 2006. Luteinization of porcine preovulatory follicles leads to systematic changes in follicular gene expression. Reproduction 132:133-145. Manandhar, G., D. Feng, Y.-J. Yi, L. Lai, T. Ezashi, J. Letko, J. Laurincik, M. Sutovsky, R.S. Prather, H. Schatten, P. Sutovsky. 2006. Centrosomal protein Centrin is not detectable during early pre-implantation development but reappears during the late blastocyst stage in porcine embryos. Reproduction 312:423-434. Price, E.M., R.S. Prather, C.M. Foley. 2006. Multipotent adult progenitor cell lines originating from green fluorescent protein transgenic swine. Stem Cells and Development 15:507-522. Hao, Y.H., H.Y. Yong, C.N. Murphy, D. Wax, M. Samuel, A. Rieke, L. Lai, Z. Liu, D.C. Durtschi, V.R. Welbern, E.M. Price, R.M. McAllister, J.R. Turk, M.H. Laughlin, R.S. Prather, E.B. Rucker. 2006. Production of endothelial nitric oxide synthase (eNOS) over-expressing piglets. Transgenic Research 15:739-750. Isom, S.C., R.S. Prather, E.B. Rucker III. 2007. Heat stress-induced apoptosis in porcine in vitro fertilized and parthenogenetic preimplantation-stage embryos. Mol. Reprod. Dev. 74:574-581. Green, J.A., J.-G. Kim, K.M. Whitworth, C. Agca, R.S. Prather. 2006. The use of microarrays to define functionally-related genes that are differentially expressed in the cycling pig uterus. In: Control of Pig Reproduction VII, Reproduction Supplement 62. Eds C.J. Ashworth and R. Kraeling. Nottingham University Press, Nottingham, UK. pp 163-176. Prather, R. S. and M.F. Smith. 2006. Reproductive Biotechnologies. In: Biotechnology Patent Law Technology, R. Brungs & M. Postiglione, eds. ITEST Faith Science Press, St. Louis, MO. pp 1-13. Seguin, M.J., R.M. Friendship, R.N. Kirkwood, A.J. Zanella and T.M. Widowski. 2006. Effect of boar presence on agonistic behaviour, shoulder scratches and stress response of sows at mixing. J. Anim. Sci. 84:1227-1237. Kirkwood, R.N., F. De Rensis, P. Silva, M. Gherpelli, B. Alegre and J.C. Dominguez. 2007. Effect of cloprostenol treatment at artificial insemination on sow fertility. Reprod. Dom. Anim. 42:26-28. Abad, M., D.J. Sprecher, P. Ross, R.M. Friendship and R.N. Kirkwood. 2007. Effect of sperm cryopreservation and supplementing semen doses with seminal plasma on the establishment of a sperm reservoir in gilts. Reprod. Dom. Anim. 42:149-152. Jansen, J., R.N. Kirkwood, A.J. Zanella and R.J. Tempelman. 2007. Influence of gestation housing on sow behaviour and fertility. J. Swine Health Prod. 15:132-136. Checura CM, JJ Parrish. 2006. The role of calcium in maturation and activation of horse oocytes . Animal Reprod. Sci. 94:340-342. Sagirkaya H, Misirlioglu M, Kaya A, First NL, Parrish JJ, Memili E. 2006. Developmental and molecular correlates of bovine preimplantation embryos. Reproduction.131(5):895-904. Braundmeier A. and Nowak R.A. 2006. Cytokines regulate matrix metalloproteinase production in human endometrial stromal cells. American Journal of Reproductive Immunology 56:201-214. Braundmeier A., Fazleabas A., Lessey B.A., Guo F., Toole B.P., Nowak R.A. 2006. Extracellular matrix metalloproteinase inducer (EMMPRIN) regulates metalloproteinases in the human endometrium. Journal of Clinical Endocrinology and Metabolism 91:2358-65. Breen, S.M., S. L. Rodriguez-Zas, R. V. Knox. 2006. Effect of PG600 and adjusted mating times on reproductive performance in weaned sows. Anim. Reprod. Sci. 93:157-164. Breen, S.M., S.L. Rodriguez-Zas, and R. V. Knox. 2006. Effect of altering dose of PG600 on reproductive performance responses in prepubertal gilts and weaned sows. Anim. Reprod. Sci. 93:157-164. Chen L., Nakai M., Belton Jr. R.J., and Nowak R.A. 2007. Expression of extracellular matrix metalloproteinase inducer and matrix metalloproteinases during mouse embryonic development. Reproduction 133:405-414. Collins E.D., W.L. Flowers, R.D. Shanks, and D.J. Miller. 2007. Porcine sperm-zona binding ability as an indicator of fertility. Anim Reprod Sci. doi:10.1016/j.anireprosci.2007.01.012 Ford, J.A., Clark, S.G., Walters, E.M., Wheeler, M.B., Hurley, W.L. 2006. Estrogenic effects of genistein on reproductive tissues of ovariectomized gilts. J. Anim. Sci. 84:834842. Jackson, A. L. S. M. Breen, S. L. Rodriguez-Zas, and R. V. Knox. 2006. Evaluation of methodology for administration of porcine FSH for use in estrus induction and for increasing ovulation rate in prepubertal gilts. Theriogenology 66:1042-1047. Marshall, K.M., Hurley, W.L., Shanks, R.D., Wheeler, M.B. 2006. Effects of suckling intensity on milk yield and piglet growth from lactation-enhanced gilts. J. Anim. Sci. 84:23462351. Nakai M., Chen L. and Nowak R.A. 2006. Tissue distribution of basigin and monocarboxylate transporter I in the adult male mouse: a study using the wild type and basigin gene knockout mice. The Anatomical Record Part A 288A:527-535. Vieira, A. and D.J. Miller. 2006. Gamete interaction: Is it species-specific? Mol Reprod Dev 73:1422-1429. Wheeler, M.B. 2007. Agricultural applications for transgenic livestock. Trends in Biotechnology 25:204- 210. Wheeler, M.B., Rutledge, J.J., Fischer-Brown, A., VanEtten, T., Malusky, S., Beebe, D.J. 2006. Application of sexed semen technology to in vitro embryo production in cattle. Theriogenology 65:219227. Wheeler, M.B., Walters, E.M. Beebe, D. J. 2007. Toward culture of single gametes: The development of microfluidic platforms for assisted reproduction. Theriogenology doi:10.1016/j.theriogenology.2007.04.042 Woodard, J., A. Hilldore, , M. B. Wheeler, S. Clark, S. Lan, R. Jamison, and A. J. Wagoner Johnson, 2007. The mechanical properties and osteoconductivity of hydroxyapatite bone scaffolds with multi-scale porosity. Biomaterials 28:4554. Zhao L.*, H. Burkin*, X. Shi, L. Li, K. Reim, and D.J. Miller. 2007. Complexin I is required for mammalian sperm acrosomal exocytosis. (*Both authors contributed equally to the paper). Submitted. Zhao L., X. Shi, L. Li, and D.J. Miller. 2007. Dynamin 2 associates with complexins and is found in the acrosomal region of mammalian sperm. Mol Reprod Dev 74:750-757. Bahr, JM, Dalponte M, Janssen SJ, Nakai M. 2006 Ion transporters for fluid resorption in the rooster (Gallus domesticus) epididymal region. Animal Reproduction Science 95 331-337. Boltz, DA, Zimmerman CR, Nakai M, Bunick D, Scherba G, Bahr JM. 2006 Epididymal stone formation and decreased sperm production in roosters vaccinated with a killed strain of avian infectious bronchitis virus. Avian Diseases 50:594-598. Jackson, U, Boltz DA, Nakai M, Bunick D, Scherba G, Bahr JM. 2006 Prepubertal exposure to the avian infectious bronchitis virus induces epididymal stones in the rooster after puberty. Journal of Poultry Science 43:280-285. VanEtten, T.L. and Wheeler, M.B. 2007. Evaluation of a novel electronic estrus detection device. Illinois Dairy Report: Getting it Right, pgs 74-76. Wheeler, M.B. 2006. Cloning and transgenic animals for agriculture. 2nd International Symposium of Applied Animal Reproduction, Londrina, Parana, Brazil, Pgs. 181-194. Wheeler, M.B. 2007. The use of gender-selected semen in dairy production schemes. Illinois Dairy Report: Getting it Right, pgs 72-73. Wheeler, M.B., D. Kim, E. Monaco, W.L. Hurley. 2007. Application of robotic and microfluidic technologies to stem cell culture and analysis. Tsukuba Meeting for Animal Biotechnology (TMAB)  Progress in Somatic Cell Nuclear Transfer Technology, Tsukuba City, Japan, Pgs. 31-32. Allan, M.F., R.M. Thallman, R.A. Cushman, S.E. Echternkamp, S.N. White, L.A. Kuehn, E. Casas, and T.P.L. Smith. 2007. Association of a single nucleotide polymorphism in SPP1 with growth traits and twinning in a cattle population selected for twinning rate. J. Anim. Sci. 85(2):341-347. Blomberg, L.A., W.M. Garrett, M. Guillomot, J.R. Miles, T.S. Sonstegard, C.P. Van Tassell, and K.A. Zuelke. 2006. Transcriptome profiling of the tubular porcine conceptus identifies the differential regulation of growth and developmentally associated genes. Mol. Reprod. Dev. 73(12):1491-1502. Casas, E., B.A. Freking, and K.A. Leymaster. 2005. Evaluation of Dorset, Finnsheep, Romanov, Texel, and Montadale breeds of sheep: V. Reproduction of F1 ewes in spring mating seasons. J. Anim. Sci. 83(12):2743-2751. Cepica, S., M. Masopust, A. Knoll, and G.A. Rohrer. 2005. (Brief Note) Linkage and RH mapping of the porcine adiponectin gene on chromosome 13. Anim. Genet. 36(3):276-277. Cepica, S., M. Masopust, A. Knoll, H. Bartenschlager, M. Yerle, G.A. Rohrer, and H. Geldermann. 2006. Linkage and RH mapping of 10 genes to a QTL region for fatness and muscling traits on pig chromosome X. Anim. Genet. 37(6):603-604. Chen, K., C. Knorr, K. Bornemann-Kolatzki, J. Ren, L. Huang, G.A. Rohrer, and B. Brenig. 2005. Targeted oligonucleotide-mediated microsatellite identification (TOMMI) from large-insert library clones. BMC Genetics 6:54. Chen, K., C. Knorr, K. Bornemann-Kolatzki, L. Huang, G.A. Rohrer, and B. Brenig. 2006. Characterization of the PGK2 associated microsatellite S0719 on SSC7 suitable for parentage and QTL diagnosis. Anim. Biotechnol. 17(1):43-49. Cushman, R.A., M.F. Allan, S.A. Jones, G.P. Rupp, and S.E. Echternkamp. 2007. Localization of Period 1 mRNA in the ruminant oocyte and investigations of its role in ovarian function. Anim. Reprod. Sci. 99(1-2):93-105. Echternkamp, S.E., K.A. Vonnahme, J.A. Green, and S.P. Ford. 2006. Increased vascular endothelial growth factor and pregnancy-associated glycoproteins, but not insulin-like growth factor-I, in maternal blood of cows gestating twin fetuses. J. Anim. Sci. 84(8):2057-2064. Ellis, R.W., G.P. Rupp, P.J. Chenoweth, L.V. Cundiff, and D.D. Lunstra. 2005. Fertility of yearling beef bulls during mating. Theriogenology 64(3):657-678. Freking, B.A., and K.A. Leymaster. 2006. Evaluation of the ovine callipyge locus: IV. Genotypic effects on reproductive traits. J. Anim. Sci. 84(2):311-316. Gargantini, G., L.V. Cundiff, D.D. Lunstra, and L.D. Van Vleck. 2005. Genetic relationships between male and female reproductive traits in beef cattle. Prof. Anim. Scientist 21:195-199. Genini, S., T.T. Nguyen, M. Malek, R. Talbot, S. Gebert, G. Rohrer, D. Nonneman, G. Stranzinger, and P. Vögeli. 2006. Radiation hybrid mapping of 18 positional and physiological candidate genes for arthrogryposis multiplex congenita on porcine chromosome 5. Anim. Genet. 37(3):239-244. Heaton, M.P., K.A. Leymaster, M.L. Clawson, B.A. Freking, and W.W. Laegreid. 2006. A freely-available set of reference DNAs for genotyping the major scrapie susceptibility alleles in sheep. Plant and Animal Genome Abstracts XIV:239. Imakawa, K., M. Imai, A. Sakai, M. Suzuki, K. Nagaoka, S. Sakai, S.-R. Lee, K.-T. Chang, S.E. Echternkamp, and R.K. Christenson. 2006. Regulation of conceptus adhesion by endometrial CXC chemokines during the implantation period in sheep. Mol. Reprod. Dev. 73(7):850-858. Isler, B.J., B.A. Freking, R.M. Thallman, M.P. Heaton, and K.A. Leymaster. 2006. Evaluation of associations between prion haplotypes and growth, carcass, and meat quality traits in a Dorset x Romanov sheep population. J. Anim. Sci. 84(4):783-788. Jacobs, K., G. Rohrer, M. Van Poucke, F. Piumi, M. Yerle, H. Barthenschlager, M. Mattheeuws, A. Van Zeveren, and L.J. Peelman. 2006. Porcine PPARGC1A (peroxisome proliferative activated receptor gamma coactivator 1A): coding sequence, genomic organization, polymorphisms and mapping. Cytogenet. Genome Res. 112(1-2):106-113. Jimenez-Severiano, H., M.J. D'Occhio, D.D. Lunstra, M.L. Mussard, T.L. Davis, W.J. Enright, and J.E. Kinder. 2007. Comparative response of rams and bulls to long-term treatment with gonadotropin-releasing hormone analogs. Anim. Reprod. Sci. 98(3-4):204-224. Kim, J.G., D. Nonneman, J.L. Vallet, T.H. Wise, and G.A. Rohrer. 2006. (Brief Note) Sequence variation and evaluation of inhibin a-subunit and steroidogenic acute regulatory protein for reproductive traits in swine. Anim. Genet. 37(4):424-425. Kim, J.G., and J.L. Vallet. 2007. Placental expression of the membrane form of folate binding protein during pregnancy in swine. Theriogenology 67(7):1279-1284. Klemcke, H.G., J.L. Vallet, and R.K. Christenson. 2006. Lack of effect of metyrapone and exogenous cortisol on early porcine conceptus development. Exp. Physiol. 91(3):521-530. Klindt, J., R.M. Thallman, and T. Wise. 2006. Effects of sire line, sire, and sex on plasma urea nitrogen, body weight, and backfat thickness in offspring of Duroc and Landrace boars. J. Anim. Sci. 84(6):1323-1330. Leeb, T., B. Brenig, and G.A. Rohrer. 2005. (Brief Note) Characterization and linkage mapping of four gene-associated porcine microsatellites. Anim. Genet. 36(3):279-280. Mousel, M.R., D.J. Nonneman, and G.A. Rohrer. 2006. Rearranged gene order between pig and human in a quantitative trait loci region on SSC3. Anim. Genet. 37(4):403-406. Murphy, S.K., C.M. Nolan, Z. Huang, K.S. Kucera, B.A. Freking, T.P.L. Smith, K.A. Leymaster, J.R. Weidman, and R.L. Jirtle. 2006. Callipyge mutation affects gene expression in cis: A potential role for chromatin structure. Genome Res. 16(3):340-346. Nonneman, D.J., T.H. Wise, J.J. Ford, L.A. 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Rogers, S. Humphray, A. Archibald, P. Chardon, D. Milan, G. Rohrer, and K. Eversole. 2005. Swine Genome Sequencing Consortium (SGSC): A strategic roadmap for sequencing the pig genome. Comp. Funct. Genom. 6(4):251-255. Shimogiri, T., S. Kiuchi, H. Hiraiwa, T. Hayashi, Y. Takano, Y. Maeda, G.A. Rohrer, D. Milan, and H. Yasue. 2006. Assignment of 204 genes localized on HSA17 to a porcine RH (IMpRH) map to generate a dense comparative map between pig and human/mouse. Cytogenet. Genome Res. 112(1-2):114-120. Tsai, S., J.P. Cassady, B.A. Freking, D.J. Nonneman, G.A. Rohrer, and J.A. Piedrahita. 2006. (Brief Note) Annotation of the Affymetrix porcine genome microarray. Anim. Genet. 37(4):423-424. Tsai, S., B. Mir, A.C. Martin, J.L. Estrada, S.R. Bischoff, W. Hsieh, J.P. Cassady, B.A. Freking, D.J. Nonneman, G.A. Rohrer, and J.A. Piedrahita. 2006. Detection of transcriptional difference of porcine imprinted genes using different microarray platforms. BMC Genomics 7:328. Vallet, J.L., and B.A. Freking. 2006. Changes in fetal organ weights during gestation after selection for ovulation rate and uterine capacity in swine. J. Anim. Sci. 84(9):2338-2345. Vizcarra, J.A., and J.J. Ford. 2006. Validation of the sperm mobility assay in boars and stallions. Theriogenology 66(5):1091-1097. Walker, S.C., R.K. Christenson, R.P. Ruiz, D.E. Reeves, S.L. Pratt, F. Arenivas, N.E. Williams, B.L. Bruner, and I.A. Polejaeva. 2007. Comparison of meat composition from offspring of cloned and conventionally produced boars. Theriogenology 67(1):178-184. Wiedmann, R.T., D.J. Nonneman, and J.W. Keele. 2006. Novel porcine repetitive elements. BMC Genomics 7:304. Williams, N.E., S.C. Walker, D.E. Reeves, E. Sherrer, J.M. Galvin, I. Polejaeva, G. Rampacek, L. Benyshek, R.K. Christenson, W.M. Graves, and S.L. Pratt. 2006. A comparison of reproductive characteristics of boars generated by somatic cell nuclear transfer to highly related conventionally produced boars. Cloning and Stem Cells 8(3):130-139. Herrick, JR, Behboodi, E, Memili, E, Blash, S, Echelard, Y., Krisher, R. Metabolism, protein content and in vitro embryonic development of goat cumulus-oocyte complexes matured with physiological concentrations of glucose and L-lactate. Mol. Reprod. Dev. 73:256-266. Published Online: 25 Oct 2005. Herrick, JR, Brad, AM, Krisher, R. Chemical manipulation of glucose metabolism in porcine oocytes: effects on nuclear and cytoplasmic maturation in vitro. Reprod. 131: 289-298. Krisher, RL, Brad, AM, Herrick, JR, Sparman, ML, Swain, JE. A comparative analysis of metabolism and viability in porcine oocytes during in vitro maturation. Anim. Reprod. Sci. 98:7296. doi:10.1016/j.anireprosci.2006.10.006 Herrick, JR, Bond, JB, Magarey, GM, Bateman, HL, Krisher, RL, Dunford, SA, Swanson, WF. Toward a feline optimized culture medium: impact of ions, carbohydrates, essential amino acids, vitamins, and serum on development and metabolism of in vitro fertilization-derived feline embryos relative to embryos grown in vivo. Biol Reprod published 31 January 2007; 10.1095/biolreprod.106.058065
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